Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045590.01.T01 | XP_003629211.1 | 95.466 | 397 | 18 | 0 | 1 | 397 | 1 | 397 | 0 | 768 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045590.01.T01 | Q3EBZ2 | 30.37 | 405 | 235 | 13 | 23 | 395 | 1 | 390 | 1.01E-41 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045590.01.T01 | G7LA96 | 95.466 | 397 | 18 | 0 | 1 | 397 | 1 | 397 | 0.0 | 768 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000650.01 | MsG0880045590.01 | 0.804269 | 2.342545e-49 | 1.202552e-46 |
| MsG0180000653.01 | MsG0880045590.01 | 0.833637 | 4.757291e-56 | 5.510956e-53 |
| MsG0180001122.01 | MsG0880045590.01 | 0.858228 | 9.514776e-63 | 2.411128e-59 |
| MsG0180001142.01 | MsG0880045590.01 | 0.814678 | 1.363275e-51 | 9.206867e-49 |
| MsG0880045491.01 | MsG0880045590.01 | 0.817230 | 3.673109e-52 | 2.659643e-49 |
| MsG0880045590.01 | MsG0880045926.01 | 0.826388 | 2.787739e-54 | 2.610806e-51 |
| MsG0880045590.01 | MsG0880046440.01 | 0.805178 | 1.513106e-49 | 7.951875e-47 |
| MsG0180005278.01 | MsG0880045590.01 | 0.835612 | 1.516607e-56 | 1.864186e-53 |
| MsG0180005279.01 | MsG0880045590.01 | 0.837573 | 4.801047e-57 | 6.262440e-54 |
| MsG0180005505.01 | MsG0880045590.01 | 0.807228 | 5.601263e-50 | 3.102833e-47 |
| MsG0780041753.01 | MsG0880045590.01 | 0.849432 | 3.246628e-60 | 6.153132e-57 |
| MsG0880042161.01 | MsG0880045590.01 | 0.822191 | 2.703264e-53 | 2.246554e-50 |
| MsG0880042434.01 | MsG0880045590.01 | 0.813822 | 2.106877e-51 | 1.390283e-48 |
| MsG0880042656.01 | MsG0880045590.01 | 0.802293 | 6.010682e-49 | 2.933514e-46 |
| MsG0880042965.01 | MsG0880045590.01 | 0.805862 | 1.087713e-49 | 5.817843e-47 |
| MsG0880043133.01 | MsG0880045590.01 | 0.801596 | 8.356016e-49 | 4.006263e-46 |
| MsG0880043207.01 | MsG0880045590.01 | 0.801477 | 8.841505e-49 | 4.226534e-46 |
| MsG0280011163.01 | MsG0880045590.01 | 0.841907 | 3.573153e-58 | 5.327688e-55 |
| MsG0380012362.01 | MsG0880045590.01 | 0.833748 | 4.463550e-56 | 5.187989e-53 |
| MsG0380012553.01 | MsG0880045590.01 | 0.802719 | 4.908639e-49 | 2.421853e-46 |
| MsG0380013068.01 | MsG0880045590.01 | 0.814663 | 1.373658e-51 | 9.273038e-49 |
| MsG0380013436.01 | MsG0880045590.01 | 0.803944 | 2.736630e-49 | 1.393329e-46 |
| MsG0380014333.01 | MsG0880045590.01 | 0.807342 | 5.297462e-50 | 2.943238e-47 |
| MsG0380014573.01 | MsG0880045590.01 | 0.810346 | 1.206761e-50 | 7.256272e-48 |
| MsG0380014894.01 | MsG0880045590.01 | 0.840871 | 6.696861e-58 | 9.668227e-55 |
| MsG0380014902.01 | MsG0880045590.01 | 0.825654 | 4.166680e-54 | 3.821358e-51 |
| MsG0680031042.01 | MsG0880045590.01 | 0.816855 | 4.458899e-52 | 3.195822e-49 |
| MsG0680034092.01 | MsG0880045590.01 | 0.809384 | 1.943009e-50 | 1.139206e-47 |
| MsG0680034782.01 | MsG0880045590.01 | 0.801873 | 7.330534e-49 | 3.539743e-46 |
| MsG0680035460.01 | MsG0880045590.01 | 0.816050 | 6.751868e-52 | 4.734142e-49 |
| MsG0480019335.01 | MsG0880045590.01 | 0.810407 | 1.170313e-50 | 7.048522e-48 |
| MsG0480020964.01 | MsG0880045590.01 | 0.809858 | 1.536892e-50 | 9.124514e-48 |
| MsG0480021927.01 | MsG0880045590.01 | 0.824265 | 8.864266e-54 | 7.812358e-51 |
| MsG0480022066.01 | MsG0880045590.01 | 0.801517 | 8.673796e-49 | 4.150643e-46 |
| MsG0480022120.01 | MsG0880045590.01 | 0.819423 | 1.170816e-52 | 9.005936e-50 |
| MsG0480022575.01 | MsG0880045590.01 | 0.838876 | 2.216219e-57 | 3.008312e-54 |
| MsG0480023491.01 | MsG0880045590.01 | 0.800533 | 1.378213e-48 | 6.432723e-46 |
| MsG0480023898.01 | MsG0880045590.01 | 0.806813 | 6.854159e-50 | 3.756352e-47 |
| MsG0480023900.01 | MsG0880045590.01 | 0.822114 | 2.816467e-53 | 2.335604e-50 |
| MsG0580024853.01 | MsG0880045590.01 | 0.868608 | 5.752991e-66 | 2.094019e-62 |
| MsG0580025739.01 | MsG0880045590.01 | 0.806656 | 7.398085e-50 | 4.037676e-47 |
| MsG0380015001.01 | MsG0880045590.01 | 0.809129 | 2.203966e-50 | 1.283426e-47 |
| MsG0380015005.01 | MsG0880045590.01 | 0.828982 | 6.641187e-55 | 6.705642e-52 |
| MsG0380015735.01 | MsG0880045590.01 | 0.835182 | 1.947109e-56 | 2.362781e-53 |
| MsG0380015852.01 | MsG0880045590.01 | 0.801540 | 8.582610e-49 | 4.109211e-46 |
| MsG0380015877.01 | MsG0880045590.01 | 0.834686 | 2.596883e-56 | 3.104319e-53 |
| MsG0380015878.01 | MsG0880045590.01 | 0.828674 | 7.882901e-55 | 7.889092e-52 |
| MsG0380015931.01 | MsG0880045590.01 | 0.802235 | 6.177585e-49 | 3.010446e-46 |
| MsG0380016061.01 | MsG0880045590.01 | 0.829128 | 6.121250e-55 | 6.208172e-52 |
| MsG0380016708.01 | MsG0880045590.01 | 0.830389 | 3.019334e-55 | 3.176525e-52 |
| MsG0380016909.01 | MsG0880045590.01 | 0.807208 | 5.654034e-50 | 3.130507e-47 |
| MsG0380017220.01 | MsG0880045590.01 | 0.834241 | 3.357758e-56 | 3.961289e-53 |
| MsG0480018128.01 | MsG0880045590.01 | 0.819010 | 1.453954e-52 | 1.105765e-49 |
| MsG0580027661.01 | MsG0880045590.01 | 0.800616 | 1.325668e-48 | 6.200623e-46 |
| MsG0580027827.01 | MsG0880045590.01 | 0.801516 | 8.678950e-49 | 4.152948e-46 |
| MsG0580027832.01 | MsG0880045590.01 | 0.805895 | 1.070300e-49 | 5.729341e-47 |
| MsG0680030629.01 | MsG0880045590.01 | 0.841515 | 4.534830e-58 | 6.679542e-55 |
| MsG0680030694.01 | MsG0880045590.01 | 0.860838 | 1.562490e-63 | 4.330021e-60 |
| MsG0680030695.01 | MsG0880045590.01 | 0.870171 | 1.785402e-66 | 6.878923e-63 |
| MsG0280007818.01 | MsG0880045590.01 | 0.825908 | 3.627392e-54 | 3.351018e-51 |
| MsG0280007826.01 | MsG0880045590.01 | 0.824942 | 6.141792e-54 | 5.519873e-51 |
| MsG0280008073.01 | MsG0880045590.01 | 0.847826 | 9.044631e-60 | 1.627611e-56 |
| MsG0780037859.01 | MsG0880045590.01 | 0.814054 | 1.873098e-51 | 1.243782e-48 |
| MsG0780038386.01 | MsG0880045590.01 | 0.802868 | 4.572966e-49 | 2.264772e-46 |
| MsG0780038825.01 | MsG0880045590.01 | 0.812069 | 5.104538e-51 | 3.213222e-48 |
| MsG0780038833.01 | MsG0880045590.01 | 0.816173 | 6.337596e-52 | 4.458395e-49 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045590.01.T01 | MTR_8g074610 | 95.466 | 397 | 18 | 0 | 1 | 397 | 1 | 397 | 0.0 | 768 |
| MsG0880045590.01.T01 | MTR_2g041510 | 77.750 | 400 | 78 | 5 | 1 | 397 | 1 | 392 | 0.0 | 603 |
| MsG0880045590.01.T01 | MTR_2g041510 | 77.368 | 380 | 76 | 4 | 20 | 397 | 1 | 372 | 0.0 | 593 |
| MsG0880045590.01.T01 | MTR_8g074640 | 75.462 | 379 | 90 | 3 | 20 | 397 | 1 | 377 | 0.0 | 590 |
| MsG0880045590.01.T01 | MTR_8g085760 | 32.905 | 389 | 213 | 12 | 21 | 383 | 9 | 375 | 1.99e-50 | 174 |
| MsG0880045590.01.T01 | MTR_6g092570 | 33.867 | 375 | 201 | 10 | 21 | 383 | 4 | 343 | 4.31e-50 | 173 |
| MsG0880045590.01.T01 | MTR_5g079830 | 33.994 | 353 | 186 | 8 | 20 | 359 | 1 | 319 | 1.24e-49 | 172 |
| MsG0880045590.01.T01 | MTR_7g096720 | 36.290 | 372 | 176 | 17 | 20 | 359 | 6 | 348 | 2.77e-47 | 166 |
| MsG0880045590.01.T01 | MTR_4g088925 | 34.316 | 373 | 189 | 12 | 33 | 383 | 34 | 372 | 6.19e-47 | 165 |
| MsG0880045590.01.T01 | MTR_3g030050 | 32.028 | 434 | 218 | 16 | 16 | 395 | 10 | 420 | 6.04e-45 | 160 |
| MsG0880045590.01.T01 | MTR_2g047180 | 28.982 | 383 | 241 | 10 | 24 | 395 | 3 | 365 | 3.36e-33 | 128 |
| MsG0880045590.01.T01 | MTR_1g016570 | 27.456 | 397 | 221 | 12 | 16 | 395 | 13 | 359 | 1.42e-31 | 123 |
| MsG0880045590.01.T01 | MTR_6g018390 | 29.609 | 358 | 194 | 14 | 23 | 359 | 3 | 323 | 1.92e-30 | 120 |
| MsG0880045590.01.T01 | MTR_1g026850 | 29.280 | 403 | 218 | 16 | 20 | 395 | 1 | 363 | 2.28e-30 | 120 |
| MsG0880045590.01.T01 | MTR_6g027100 | 28.818 | 406 | 223 | 17 | 24 | 396 | 4 | 376 | 1.78e-29 | 119 |
| MsG0880045590.01.T01 | MTR_3g029650 | 28.571 | 357 | 188 | 11 | 24 | 359 | 4 | 314 | 3.62e-29 | 120 |
| MsG0880045590.01.T01 | MTR_3g029650 | 26.343 | 391 | 238 | 12 | 16 | 376 | 364 | 734 | 1.36e-24 | 106 |
| MsG0880045590.01.T01 | MTR_1g007260 | 28.496 | 379 | 212 | 13 | 24 | 375 | 21 | 367 | 1.05e-28 | 115 |
| MsG0880045590.01.T01 | MTR_3g030360 | 28.030 | 396 | 216 | 15 | 22 | 395 | 2 | 350 | 3.18e-28 | 114 |
| MsG0880045590.01.T01 | MTR_6g027040 | 27.792 | 403 | 233 | 17 | 24 | 397 | 4 | 377 | 4.48e-28 | 114 |
| MsG0880045590.01.T01 | MTR_3g058460 | 28.974 | 390 | 229 | 14 | 25 | 395 | 15 | 375 | 6.04e-28 | 113 |
| MsG0880045590.01.T01 | MTR_3g029620 | 27.895 | 380 | 221 | 13 | 23 | 381 | 1 | 348 | 1.61e-25 | 107 |
| MsG0880045590.01.T01 | MTR_6g018360 | 27.078 | 373 | 216 | 13 | 24 | 374 | 4 | 342 | 2.66e-25 | 107 |
| MsG0880045590.01.T01 | MTR_3g030380 | 27.273 | 374 | 217 | 13 | 24 | 379 | 4 | 340 | 3.47e-25 | 105 |
| MsG0880045590.01.T01 | MTR_3g030080 | 26.316 | 361 | 201 | 10 | 25 | 361 | 7 | 326 | 5.50e-25 | 105 |
| MsG0880045590.01.T01 | MTR_1g049170 | 25.352 | 426 | 225 | 15 | 20 | 395 | 1 | 383 | 6.82e-25 | 105 |
| MsG0880045590.01.T01 | MTR_3g029640 | 27.559 | 381 | 209 | 15 | 24 | 381 | 3 | 339 | 2.02e-24 | 103 |
| MsG0880045590.01.T01 | MTR_4g125090 | 26.946 | 334 | 169 | 11 | 21 | 340 | 41 | 313 | 4.03e-24 | 102 |
| MsG0880045590.01.T01 | MTR_3g037710 | 31.418 | 261 | 129 | 8 | 140 | 376 | 9 | 243 | 2.20e-23 | 99.0 |
| MsG0880045590.01.T01 | MTR_3g030480 | 26.596 | 376 | 215 | 13 | 24 | 379 | 4 | 338 | 3.14e-23 | 100 |
| MsG0880045590.01.T01 | MTR_3g114990 | 28.291 | 357 | 219 | 12 | 24 | 363 | 22 | 358 | 4.01e-23 | 100 |
| MsG0880045590.01.T01 | MTR_3g030355 | 27.363 | 402 | 232 | 18 | 20 | 382 | 1 | 381 | 2.11e-22 | 98.2 |
| MsG0880045590.01.T01 | MTR_3g029920 | 28.643 | 398 | 216 | 15 | 24 | 394 | 8 | 364 | 2.85e-22 | 97.8 |
| MsG0880045590.01.T01 | MTR_3g091990 | 27.348 | 362 | 210 | 13 | 24 | 359 | 6 | 340 | 3.89e-22 | 97.8 |
| MsG0880045590.01.T01 | MTR_3g030440 | 26.053 | 380 | 220 | 13 | 20 | 379 | 1 | 339 | 1.04e-21 | 95.9 |
| MsG0880045590.01.T01 | MTR_3g029840 | 26.420 | 352 | 207 | 14 | 25 | 361 | 5 | 319 | 1.55e-21 | 95.1 |
| MsG0880045590.01.T01 | MTR_6g018400 | 26.257 | 358 | 203 | 10 | 22 | 357 | 2 | 320 | 3.76e-21 | 94.4 |
| MsG0880045590.01.T01 | MTR_3g029670 | 27.518 | 407 | 210 | 16 | 24 | 382 | 4 | 373 | 4.18e-21 | 94.4 |
| MsG0880045590.01.T01 | MTR_3g114970 | 26.098 | 387 | 208 | 16 | 24 | 382 | 3 | 339 | 8.99e-21 | 93.2 |
| MsG0880045590.01.T01 | MTR_6g018315 | 25.888 | 394 | 229 | 14 | 37 | 396 | 2 | 366 | 1.76e-20 | 92.8 |
| MsG0880045590.01.T01 | MTR_3g029950 | 27.838 | 370 | 188 | 16 | 25 | 365 | 5 | 324 | 3.02e-20 | 91.7 |
| MsG0880045590.01.T01 | MTR_3g092000 | 25.679 | 405 | 216 | 16 | 24 | 381 | 6 | 372 | 3.48e-20 | 92.0 |
| MsG0880045590.01.T01 | MTR_3g030560 | 26.290 | 407 | 228 | 18 | 24 | 395 | 3 | 372 | 1.48e-19 | 89.7 |
| MsG0880045590.01.T01 | MTR_3g029750 | 25.326 | 383 | 216 | 12 | 24 | 370 | 4 | 352 | 1.47e-18 | 87.0 |
| MsG0880045590.01.T01 | MTR_3g047980 | 28.191 | 376 | 219 | 14 | 25 | 395 | 30 | 359 | 4.29e-17 | 82.4 |
| MsG0880045590.01.T01 | MTR_3g029900 | 31.214 | 173 | 83 | 5 | 218 | 387 | 297 | 436 | 6.02e-15 | 76.6 |
| MsG0880045590.01.T01 | MTR_1g016540 | 29.630 | 189 | 103 | 6 | 225 | 395 | 2 | 178 | 4.91e-13 | 67.8 |
| MsG0880045590.01.T01 | MTR_6g018370 | 34.884 | 129 | 74 | 4 | 210 | 337 | 132 | 251 | 6.24e-12 | 65.9 |
| MsG0880045590.01.T01 | MTR_1g007250 | 34.722 | 144 | 77 | 5 | 227 | 367 | 4 | 133 | 1.49e-11 | 62.8 |
| MsG0880045590.01.T01 | MTR_5g042230 | 32.895 | 152 | 88 | 5 | 210 | 359 | 13 | 152 | 1.76e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880045590.01.T01 | AT2G17030 | 30.370 | 405 | 235 | 13 | 23 | 395 | 1 | 390 | 1.03e-42 | 154 |
| MsG0880045590.01.T01 | AT2G17036 | 27.764 | 407 | 237 | 14 | 23 | 395 | 1 | 384 | 2.08e-36 | 137 |
| MsG0880045590.01.T01 | AT2G17036 | 27.833 | 406 | 236 | 14 | 24 | 395 | 1 | 383 | 3.44e-36 | 137 |
| MsG0880045590.01.T01 | AT1G65770 | 27.869 | 366 | 216 | 10 | 23 | 372 | 1 | 334 | 8.75e-35 | 132 |
| MsG0880045590.01.T01 | AT4G35733 | 29.049 | 389 | 213 | 16 | 20 | 384 | 1 | 350 | 2.69e-32 | 125 |
| MsG0880045590.01.T01 | AT2G26160 | 29.429 | 350 | 200 | 13 | 25 | 361 | 5 | 320 | 5.55e-29 | 115 |
| MsG0880045590.01.T01 | AT1G44080 | 28.261 | 368 | 211 | 13 | 24 | 381 | 4 | 328 | 6.77e-29 | 115 |
| MsG0880045590.01.T01 | AT3G25750 | 28.150 | 373 | 217 | 13 | 22 | 376 | 2 | 341 | 4.76e-27 | 110 |
| MsG0880045590.01.T01 | AT3G25750 | 27.763 | 371 | 207 | 13 | 22 | 376 | 2 | 327 | 2.70e-25 | 105 |
| MsG0880045590.01.T01 | AT2G17690 | 28.436 | 422 | 217 | 15 | 25 | 379 | 3 | 406 | 3.07e-25 | 106 |
| MsG0880045590.01.T01 | AT1G65740 | 27.660 | 376 | 216 | 14 | 23 | 372 | 1 | 346 | 8.28e-23 | 99.4 |
| MsG0880045590.01.T01 | AT1G65760 | 27.824 | 363 | 216 | 13 | 25 | 372 | 5 | 336 | 2.45e-22 | 97.8 |
| MsG0880045590.01.T01 | AT2G16290 | 27.160 | 405 | 230 | 18 | 23 | 383 | 1 | 384 | 3.86e-20 | 92.0 |
| MsG0880045590.01.T01 | AT5G24040 | 23.797 | 374 | 206 | 14 | 23 | 363 | 1 | 328 | 6.66e-15 | 75.9 |
| MsG0880045590.01.T01 | AT1G64840 | 24.789 | 355 | 214 | 13 | 25 | 359 | 4 | 325 | 2.82e-13 | 71.2 |
| MsG0880045590.01.T01 | AT2G16300 | 23.881 | 335 | 195 | 14 | 23 | 322 | 1 | 310 | 9.89e-12 | 65.9 |
Find 72 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATGTTCGTATGTATAATT+TGG | 0.212475 | 8:-61431653 | MsG0880045590.01.T01:CDS |
| TCACCTCCTTTGTGTGGATT+TGG | 0.230521 | 8:-61431961 | MsG0880045590.01.T01:CDS |
| AAGGGAACTGCATCTACTTT+AGG | 0.247416 | 8:-61431684 | MsG0880045590.01.T01:CDS |
| ATCTTTCAAGAGGGTAAATT+AGG | 0.254641 | 8:-61432138 | MsG0880045590.01.T01:CDS |
| GTCTCAACTAAAGACTTAAT+TGG | 0.265830 | 8:-61431712 | MsG0880045590.01.T01:CDS |
| TGGATGAAGAATGGGGAAAA+TGG | 0.270862 | 8:-61431795 | MsG0880045590.01.T01:CDS |
| TTCCAAAGGTTGGCTGATTA+AGG | 0.291497 | 8:-61432403 | MsG0880045590.01.T01:CDS |
| AATGGAAAAGAAGGAGAGTT+TGG | 0.332443 | 8:+61432546 | None:intergenic |
| AGGTGCTAAAGGTTCTAAAT+TGG | 0.350268 | 8:+61432431 | None:intergenic |
| TATGGAACTTGTTGGATCAT+AGG | 0.360148 | 8:-61432284 | MsG0880045590.01.T01:CDS |
| ATAGGACTCAACAAACTTAC+AGG | 0.370665 | 8:+61432360 | None:intergenic |
| ATAAATGGGGAACTATTTCA+TGG | 0.373052 | 8:-61432017 | MsG0880045590.01.T01:CDS |
| AGGGAACTGCATCTACTTTA+GGG | 0.379511 | 8:-61431683 | MsG0880045590.01.T01:CDS |
| GAAGGAGAGTTTGGATGAGA+TGG | 0.382409 | 8:+61432555 | None:intergenic |
| CTCAACTTCCTATAGAACTA+TGG | 0.385206 | 8:-61432662 | MsG0880045590.01.T01:CDS |
| CAATGATATAAACGGTTGAT+TGG | 0.387856 | 8:+61432478 | None:intergenic |
| ACACTAGTCCAAGGAGAGTT+AGG | 0.390020 | 8:+61432183 | None:intergenic |
| TTATAAGTTGGATGAAGAAT+GGG | 0.394790 | 8:-61431803 | MsG0880045590.01.T01:CDS |
| TACCTTAATCAGCCAACCTT+TGG | 0.397147 | 8:+61432401 | None:intergenic |
| TTTATAAGTTGGATGAAGAA+TGG | 0.397683 | 8:-61431804 | MsG0880045590.01.T01:CDS |
| CACCTTCTTCTTCCAAAGGT+TGG | 0.399560 | 8:-61432413 | MsG0880045590.01.T01:CDS |
| AAAGACTTAATTGGATATCA+AGG | 0.406036 | 8:-61431703 | MsG0880045590.01.T01:CDS |
| GGATGATCATAGAATCACTA+TGG | 0.406868 | 8:-61431632 | MsG0880045590.01.T01:CDS |
| TGGAAACAAGAAGCATTTGA+TGG | 0.415140 | 8:-61431941 | MsG0880045590.01.T01:CDS |
| TGTTCTATCCTAACTCTCCT+TGG | 0.421475 | 8:-61432191 | MsG0880045590.01.T01:CDS |
| ATCTTAGGCCATAGTTCTAT+AGG | 0.430698 | 8:+61432654 | None:intergenic |
| AGTTGGTTCAATGATATAAA+CGG | 0.439030 | 8:+61432470 | None:intergenic |
| TACCATGCTATGGAACTTGT+TGG | 0.443560 | 8:-61432292 | MsG0880045590.01.T01:CDS |
| ATCTGATTCTTCTTCATCCA+TGG | 0.450631 | 8:-61432703 | MsG0880045590.01.T01:CDS |
| TGACGATGTTATTGTGTTCA+AGG | 0.452162 | 8:-61432058 | MsG0880045590.01.T01:CDS |
| ATAGAACTATGGCCTAAGAT+AGG | 0.452927 | 8:-61432651 | MsG0880045590.01.T01:CDS |
| TTGTTTAGCGAGTCGTAGAC+AGG | 0.454181 | 8:+61432510 | None:intergenic |
| CTTGTATTGACAAGACTTTG+TGG | 0.456773 | 8:-61431597 | MsG0880045590.01.T01:CDS |
| ATGTTTCAAAGTTTATAAGT+TGG | 0.473392 | 8:-61431815 | MsG0880045590.01.T01:CDS |
| GGATGAAGAATGGGGAAAAT+GGG | 0.473828 | 8:-61431794 | MsG0880045590.01.T01:CDS |
| GGAGAGTTTGGATGAGATGG+AGG | 0.480906 | 8:+61432558 | None:intergenic |
| CTTTGTGGTCTCGTTCACCT+TGG | 0.488154 | 8:-61431582 | MsG0880045590.01.T01:CDS |
| ATGGAACTTGTTGGATCATA+GGG | 0.493145 | 8:-61432283 | MsG0880045590.01.T01:CDS |
| TTGGAAGAAGAAGGTGCTAA+AGG | 0.501406 | 8:+61432420 | None:intergenic |
| ACAGGGTGAGGTATTTGTAA+TGG | 0.505888 | 8:+61432528 | None:intergenic |
| CACAAAGTCTTGTCAATACA+AGG | 0.513043 | 8:+61431598 | None:intergenic |
| GGTATTTGTAATGGAAAAGA+AGG | 0.516150 | 8:+61432537 | None:intergenic |
| TAAAGGTTCTAAATTGGAGT+TGG | 0.521984 | 8:+61432437 | None:intergenic |
| GAAGTGTTGTTACTGCTAAG+AGG | 0.538395 | 8:+61432315 | None:intergenic |
| CAATTCTCACCTCCTTTGTG+TGG | 0.538436 | 8:-61431967 | MsG0880045590.01.T01:CDS |
| GTTTGGATGAGATGGAGGAA+TGG | 0.540037 | 8:+61432563 | None:intergenic |
| CTATGAAAGTAGTGAAGACA+TGG | 0.542033 | 8:-61431884 | MsG0880045590.01.T01:CDS |
| CTCTCCTTGGACTAGTGTTG+AGG | 0.545956 | 8:-61432178 | MsG0880045590.01.T01:CDS |
| CCAATCAACTATCTCATCCA+TGG | 0.548037 | 8:+61432686 | None:intergenic |
| CCATGGATGAGATAGTTGAT+TGG | 0.548664 | 8:-61432686 | MsG0880045590.01.T01:CDS |
| ACAACACTTCTACCATGCTA+TGG | 0.549319 | 8:-61432302 | MsG0880045590.01.T01:CDS |
| TTAGCACCTTCTTCTTCCAA+AGG | 0.559083 | 8:-61432417 | MsG0880045590.01.T01:CDS |
| AGCCAACCTTTGGAAGAAGA+AGG | 0.565959 | 8:+61432411 | None:intergenic |
| ATCCAACAAGTTCCATAGCA+TGG | 0.567906 | 8:+61432290 | None:intergenic |
| GACGATGTTATTGTGTTCAA+GGG | 0.569592 | 8:-61432057 | MsG0880045590.01.T01:CDS |
| AAGTGTTGTTACTGCTAAGA+GGG | 0.570047 | 8:+61432316 | None:intergenic |
| AGCGAGTCGTAGACAGGGTG+AGG | 0.572671 | 8:+61432516 | None:intergenic |
| TATAAGTTGGATGAAGAATG+GGG | 0.581944 | 8:-61431802 | MsG0880045590.01.T01:CDS |
| GCCATCTAACATCTCATCCA+AGG | 0.582881 | 8:+61431565 | None:intergenic |
| GAGTTGGAACTTGCATCAGT+TGG | 0.584443 | 8:+61432453 | None:intergenic |
| AGCTTGTTGTATCTTTCAAG+AGG | 0.595503 | 8:-61432148 | MsG0880045590.01.T01:CDS |
| AAAGTAGTGAAGACATGGGA+AGG | 0.596556 | 8:-61431879 | MsG0880045590.01.T01:CDS |
| AAGACTTAATTGGATATCAA+GGG | 0.598333 | 8:-61431702 | MsG0880045590.01.T01:CDS |
| GCTTGTTGTATCTTTCAAGA+GGG | 0.601791 | 8:-61432147 | MsG0880045590.01.T01:CDS |
| TGTTTAGCGAGTCGTAGACA+GGG | 0.623455 | 8:+61432511 | None:intergenic |
| AAATTAGGATTCATGAAACA+TGG | 0.629944 | 8:-61432123 | MsG0880045590.01.T01:CDS |
| TTTCCAAATCCACACAAAGG+AGG | 0.638096 | 8:+61431958 | None:intergenic |
| CAATCCTCAACACTAGTCCA+AGG | 0.639391 | 8:+61432174 | None:intergenic |
| CTACGATCGAGACACTGATG+TGG | 0.644374 | 8:-61431839 | MsG0880045590.01.T01:CDS |
| TTGTTTCCAAATCCACACAA+AGG | 0.650065 | 8:+61431955 | None:intergenic |
| AAGCATTTGATGGAGTCATG+TGG | 0.661502 | 8:-61431931 | MsG0880045590.01.T01:CDS |
| TATGAAAGTAGTGAAGACAT+GGG | 0.714849 | 8:-61431883 | MsG0880045590.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTTTCAAAGTTTATAAGT+TGG | - | Chr8:61432496-61432515 | MsG0880045590.01.T01:CDS | 20.0% |
| !!! | AGTTTTATGTTACTGATAAA+TGG | - | Chr8:61432279-61432298 | MsG0880045590.01.T01:CDS | 20.0% |
| !!! | GTTTTATGTTACTGATAAAT+GGG | - | Chr8:61432280-61432299 | MsG0880045590.01.T01:CDS | 20.0% |
| !!! | TTTTATGTTACTGATAAATG+GGG | - | Chr8:61432281-61432300 | MsG0880045590.01.T01:CDS | 20.0% |
| ! | AAAGACTTAATTGGATATCA+AGG | - | Chr8:61432608-61432627 | MsG0880045590.01.T01:CDS | 25.0% |
| ! | AAATTAGGATTCATGAAACA+TGG | - | Chr8:61432188-61432207 | MsG0880045590.01.T01:CDS | 25.0% |
| ! | AAGACTTAATTGGATATCAA+GGG | - | Chr8:61432609-61432628 | MsG0880045590.01.T01:CDS | 25.0% |
| ! | TGATGTTCGTATGTATAATT+TGG | - | Chr8:61432658-61432677 | MsG0880045590.01.T01:CDS | 25.0% |
| !! | AGTTGGTTCAATGATATAAA+CGG | + | Chr8:61431844-61431863 | None:intergenic | 25.0% |
| !! | TCAAGATATTTTCCTATCTT+AGG | + | Chr8:61431675-61431694 | None:intergenic | 25.0% |
| !! | TTATAAGTTGGATGAAGAAT+GGG | - | Chr8:61432508-61432527 | MsG0880045590.01.T01:CDS | 25.0% |
| !! | TTTATAAGTTGGATGAAGAA+TGG | - | Chr8:61432507-61432526 | MsG0880045590.01.T01:CDS | 25.0% |
| !!! | TAAGAGGGTATGATAATTTT+CGG | + | Chr8:61431983-61432002 | None:intergenic | 25.0% |
| ATAAATGGGGAACTATTTCA+TGG | - | Chr8:61432294-61432313 | MsG0880045590.01.T01:CDS | 30.0% | |
| ATCTTTCAAGAGGGTAAATT+AGG | - | Chr8:61432173-61432192 | MsG0880045590.01.T01:CDS | 30.0% | |
| CAATGATATAAACGGTTGAT+TGG | + | Chr8:61431836-61431855 | None:intergenic | 30.0% | |
| GTCTCAACTAAAGACTTAAT+TGG | - | Chr8:61432599-61432618 | MsG0880045590.01.T01:CDS | 30.0% | |
| TATGAAAGTAGTGAAGACAT+GGG | - | Chr8:61432428-61432447 | MsG0880045590.01.T01:CDS | 30.0% | |
| ! | GGTATTTGTAATGGAAAAGA+AGG | + | Chr8:61431777-61431796 | None:intergenic | 30.0% |
| ! | TATAAGTTGGATGAAGAATG+GGG | - | Chr8:61432509-61432528 | MsG0880045590.01.T01:CDS | 30.0% |
| ! | TGAAACATGGTGATGAAAAA+TGG | - | Chr8:61432201-61432220 | MsG0880045590.01.T01:CDS | 30.0% |
| !! | TAAAGGTTCTAAATTGGAGT+TGG | + | Chr8:61431877-61431896 | None:intergenic | 30.0% |
| AATGGAAAAGAAGGAGAGTT+TGG | + | Chr8:61431768-61431787 | None:intergenic | 35.0% | |
| ATAGAACTATGGCCTAAGAT+AGG | - | Chr8:61431660-61431679 | MsG0880045590.01.T01:CDS | 35.0% | |
| ATAGGACTCAACAAACTTAC+AGG | + | Chr8:61431954-61431973 | None:intergenic | 35.0% | |
| ATCTGATTCTTCTTCATCCA+TGG | - | Chr8:61431608-61431627 | MsG0880045590.01.T01:CDS | 35.0% | |
| CTATGAAAGTAGTGAAGACA+TGG | - | Chr8:61432427-61432446 | MsG0880045590.01.T01:CDS | 35.0% | |
| CTCAACTTCCTATAGAACTA+TGG | - | Chr8:61431649-61431668 | MsG0880045590.01.T01:CDS | 35.0% | |
| GACGATGTTATTGTGTTCAA+GGG | - | Chr8:61432254-61432273 | MsG0880045590.01.T01:CDS | 35.0% | |
| GGATGATCATAGAATCACTA+TGG | - | Chr8:61432679-61432698 | MsG0880045590.01.T01:CDS | 35.0% | |
| TGACGATGTTATTGTGTTCA+AGG | - | Chr8:61432253-61432272 | MsG0880045590.01.T01:CDS | 35.0% | |
| TGATGAAAAATGGACACTTG+TGG | - | Chr8:61432211-61432230 | MsG0880045590.01.T01:CDS | 35.0% | |
| TTGTTTCCAAATCCACACAA+AGG | + | Chr8:61432359-61432378 | None:intergenic | 35.0% | |
| ! | AAGTGTTGTTACTGCTAAGA+GGG | + | Chr8:61431998-61432017 | None:intergenic | 35.0% |
| ! | ATCTTAGGCCATAGTTCTAT+AGG | + | Chr8:61431660-61431679 | None:intergenic | 35.0% |
| ! | CACAAAGTCTTGTCAATACA+AGG | + | Chr8:61432716-61432735 | None:intergenic | 35.0% |
| ! | CTTGTATTGACAAGACTTTG+TGG | - | Chr8:61432714-61432733 | MsG0880045590.01.T01:CDS | 35.0% |
| ! | GATAATTTTCGGTCAGAGAT+AGG | + | Chr8:61431972-61431991 | None:intergenic | 35.0% |
| !! | AGCTTGTTGTATCTTTCAAG+AGG | - | Chr8:61432163-61432182 | MsG0880045590.01.T01:CDS | 35.0% |
| !! | AGGTGCTAAAGGTTCTAAAT+TGG | + | Chr8:61431883-61431902 | None:intergenic | 35.0% |
| !! | ATGGAACTTGTTGGATCATA+GGG | - | Chr8:61432028-61432047 | MsG0880045590.01.T01:CDS | 35.0% |
| !! | GCTTGTTGTATCTTTCAAGA+GGG | - | Chr8:61432164-61432183 | MsG0880045590.01.T01:CDS | 35.0% |
| !! | TATGGAACTTGTTGGATCAT+AGG | - | Chr8:61432027-61432046 | MsG0880045590.01.T01:CDS | 35.0% |
| !! | TGGAAACAAGAAGCATTTGA+TGG | - | Chr8:61432370-61432389 | MsG0880045590.01.T01:CDS | 35.0% |
| AAAGTAGTGAAGACATGGGA+AGG | - | Chr8:61432432-61432451 | MsG0880045590.01.T01:CDS | 40.0% | |
| AAGCATTTGATGGAGTCATG+TGG | - | Chr8:61432380-61432399 | MsG0880045590.01.T01:CDS | 40.0% | |
| AAGGGAACTGCATCTACTTT+AGG | - | Chr8:61432627-61432646 | MsG0880045590.01.T01:CDS | 40.0% | |
| ACAACACTTCTACCATGCTA+TGG | - | Chr8:61432009-61432028 | MsG0880045590.01.T01:CDS | 40.0% | |
| AGGGAACTGCATCTACTTTA+GGG | - | Chr8:61432628-61432647 | MsG0880045590.01.T01:CDS | 40.0% | |
| ATCCAACAAGTTCCATAGCA+TGG | + | Chr8:61432024-61432043 | None:intergenic | 40.0% | |
| CCAATCAACTATCTCATCCA+TGG | + | Chr8:61431628-61431647 | None:intergenic | 40.0% | |
| CCATGGATGAGATAGTTGAT+TGG | - | Chr8:61431625-61431644 | MsG0880045590.01.T01:CDS | 40.0% | |
| GGATGAAGAATGGGGAAAAT+GGG | - | Chr8:61432517-61432536 | MsG0880045590.01.T01:CDS | 40.0% | |
| TACCATGCTATGGAACTTGT+TGG | - | Chr8:61432019-61432038 | MsG0880045590.01.T01:CDS | 40.0% | |
| TACCTTAATCAGCCAACCTT+TGG | + | Chr8:61431913-61431932 | None:intergenic | 40.0% | |
| TGGATGAAGAATGGGGAAAA+TGG | - | Chr8:61432516-61432535 | MsG0880045590.01.T01:CDS | 40.0% | |
| TGTTCTATCCTAACTCTCCT+TGG | - | Chr8:61432120-61432139 | MsG0880045590.01.T01:CDS | 40.0% | |
| TTAGCACCTTCTTCTTCCAA+AGG | - | Chr8:61431894-61431913 | MsG0880045590.01.T01:CDS | 40.0% | |
| TTTCCAAATCCACACAAAGG+AGG | + | Chr8:61432356-61432375 | None:intergenic | 40.0% | |
| ! | ACAGGGTGAGGTATTTGTAA+TGG | + | Chr8:61431786-61431805 | None:intergenic | 40.0% |
| ! | TTCCAAAGGTTGGCTGATTA+AGG | - | Chr8:61431908-61431927 | MsG0880045590.01.T01:CDS | 40.0% |
| !! | GAAGTGTTGTTACTGCTAAG+AGG | + | Chr8:61431999-61432018 | None:intergenic | 40.0% |
| !! | TTGGAAGAAGAAGGTGCTAA+AGG | + | Chr8:61431894-61431913 | None:intergenic | 40.0% |
| ACACTAGTCCAAGGAGAGTT+AGG | + | Chr8:61432131-61432150 | None:intergenic | 45.0% | |
| CAATCCTCAACACTAGTCCA+AGG | + | Chr8:61432140-61432159 | None:intergenic | 45.0% | |
| CAATTCTCACCTCCTTTGTG+TGG | - | Chr8:61432344-61432363 | MsG0880045590.01.T01:CDS | 45.0% | |
| CACCTTCTTCTTCCAAAGGT+TGG | - | Chr8:61431898-61431917 | MsG0880045590.01.T01:CDS | 45.0% | |
| GAAGGAGAGTTTGGATGAGA+TGG | + | Chr8:61431759-61431778 | None:intergenic | 45.0% | |
| GAGTTGGAACTTGCATCAGT+TGG | + | Chr8:61431861-61431880 | None:intergenic | 45.0% | |
| GTTTGGATGAGATGGAGGAA+TGG | + | Chr8:61431751-61431770 | None:intergenic | 45.0% | |
| TCACCTCCTTTGTGTGGATT+TGG | - | Chr8:61432350-61432369 | MsG0880045590.01.T01:CDS | 45.0% | |
| TGTTTAGCGAGTCGTAGACA+GGG | + | Chr8:61431803-61431822 | None:intergenic | 45.0% | |
| TTGTTTAGCGAGTCGTAGAC+AGG | + | Chr8:61431804-61431823 | None:intergenic | 45.0% | |
| ! | AGCCAACCTTTGGAAGAAGA+AGG | + | Chr8:61431903-61431922 | None:intergenic | 45.0% |
| CTACGATCGAGACACTGATG+TGG | - | Chr8:61432472-61432491 | MsG0880045590.01.T01:CDS | 50.0% | |
| CTTTGTGGTCTCGTTCACCT+TGG | - | Chr8:61432729-61432748 | MsG0880045590.01.T01:CDS | 50.0% | |
| GGAGAGTTTGGATGAGATGG+AGG | + | Chr8:61431756-61431775 | None:intergenic | 50.0% | |
| !! | CTCTCCTTGGACTAGTGTTG+AGG | - | Chr8:61432133-61432152 | MsG0880045590.01.T01:CDS | 50.0% |
| AGCGAGTCGTAGACAGGGTG+AGG | + | Chr8:61431798-61431817 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 61431570 | 61432763 | 61431570 | ID=MsG0880045590.01;Name=MsG0880045590.01 |
| Chr8 | mRNA | 61431570 | 61432763 | 61431570 | ID=MsG0880045590.01.T01;Parent=MsG0880045590.01;Name=MsG0880045590.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|397 |
| Chr8 | exon | 61431570 | 61432763 | 61431570 | ID=MsG0880045590.01.T01:exon:23486;Parent=MsG0880045590.01.T01 |
| Chr8 | CDS | 61431570 | 61432763 | 61431570 | ID=MsG0880045590.01.T01:cds;Parent=MsG0880045590.01.T01 |
| Gene Sequence |
| Protein sequence |
