AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380016296.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016296.01.T01 MTR_3g087250 99.666 299 1 0 1 299 1 299 0.0 603
MsG0380016296.01.T01 MTR_3g074260 59.344 305 96 7 3 299 4 288 1.19e-107 315
MsG0380016296.01.T01 MTR_0276s0040 50.321 312 115 6 5 296 4 295 3.42e-89 270
MsG0380016296.01.T01 MTR_3g074260 59.483 232 75 5 71 299 1 216 3.10e-79 239
MsG0380016296.01.T01 MTR_3g074260 60.891 202 63 5 3 195 4 198 3.68e-72 221
MsG0380016296.01.T01 MTR_3g036400 58.065 62 23 1 71 129 1 62 1.36e-14 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380016296.01.T01 AT4G39860 62.963 297 109 1 3 299 4 299 5.49e-127 364
MsG0380016296.01.T01 AT4G39860 62.963 297 109 1 3 299 4 299 5.49e-127 364
MsG0380016296.01.T01 AT4G39860 62.963 297 109 1 3 299 4 299 5.49e-127 364
MsG0380016296.01.T01 AT4G39860 62.626 297 109 2 3 299 4 298 2.72e-124 357
MsG0380016296.01.T01 AT1G78150 52.508 299 112 5 3 299 4 274 1.59e-90 271
MsG0380016296.01.T01 AT1G78150 52.508 299 112 5 3 299 4 274 1.59e-90 271
MsG0380016296.01.T01 AT1G35780 50.980 306 118 7 3 299 4 286 6.95e-87 262
MsG0380016296.01.T01 AT1G78150 47.866 328 112 6 3 299 4 303 3.24e-84 255
MsG0380016296.01.T01 AT2G22270 44.615 325 140 11 10 299 9 328 1.40e-69 219
MsG0380016296.01.T01 AT2G22270 45.423 284 116 10 51 299 10 289 2.98e-59 191
MsG0380016296.01.T01 AT1G35780 49.367 237 87 7 71 299 1 212 1.08e-58 187
MsG0380016296.01.T01 AT1G35780 49.367 237 87 7 71 299 1 212 1.08e-58 187
MsG0380016296.01.T01 AT1G35780 49.367 237 87 7 71 299 1 212 1.08e-58 187

Find 92 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCGATGTCCTCAAATTTC+TGG 0.079733 3:+81648816 None:intergenic
TTAACAGGGTCTTTGATTTC+TGG 0.136628 3:+81647572 None:intergenic
CAGAAGTTTGCAGAGCTTTC+AGG 0.210481 3:-81647701 MsG0380016296.01.T01:CDS
GGGCAAAGTAATATCTTATT+TGG 0.218414 3:-81647764 MsG0380016296.01.T01:CDS
CTCTCAGCTTTGCCCTGCTC+AGG 0.267205 3:+81647630 None:intergenic
GAAATCAAAGACCCTGTTAA+AGG 0.278225 3:-81647569 MsG0380016296.01.T01:CDS
GGTATAGAGGTAGGCTTCTT+AGG 0.279404 3:+81649042 None:intergenic
CTCACTGGAAATGATATATT+TGG 0.293153 3:-81648925 MsG0380016296.01.T01:CDS
GGTTTGCGGGCACCTTTAAC+AGG 0.293722 3:+81647557 None:intergenic
TTCTTTGATGTCTTCTCAAC+AGG 0.305067 3:+81647734 None:intergenic
ATGGAACGCGGTACAATTTC+AGG 0.309840 3:+81648895 None:intergenic
TCCAGTGAGCTCCTTGGTCT+TGG 0.319341 3:+81648939 None:intergenic
GCATCTCAGCAAGCAATAAA+TGG 0.332847 3:-81649105 MsG0380016296.01.T01:intron
AATTCCGTGTGAACATTAGT+AGG 0.335907 3:-81647448 MsG0380016296.01.T01:three_prime_UTR
CTGAAAGCTCTGCAAACTTC+TGG 0.342314 3:+81647702 None:intergenic
CTTAGGACTAACACTTTCTT+CGG 0.378680 3:+81649059 None:intergenic
AACTAGCATACGCATATATC+AGG 0.379428 3:-81649259 MsG0380016296.01.T01:intron
CATGGCAGCTGCACGAACTT+TGG 0.379756 3:-81648875 MsG0380016296.01.T01:CDS
ACCGATGTCCTCAAATTTCT+GGG 0.398231 3:+81648817 None:intergenic
ATGTTCAATTATCAGCATCT+AGG 0.418088 3:-81647419 MsG0380016296.01.T01:three_prime_UTR
TTCCTGTAATTGTAGCCTGC+TGG 0.421397 3:-81647788 MsG0380016296.01.T01:intron
ACTTTCAGCAAGCCATGCTC+AGG 0.438294 3:-81649369 MsG0380016296.01.T01:intron
TGGGACTTTGCAAACTGACT+TGG 0.452037 3:-81648992 MsG0380016296.01.T01:CDS
TTCACACTCAAAACAACAAT+GGG 0.456072 3:-81650425 MsG0380016296.01.T01:exon
TCTCAGCTTTGCCCTGCTCA+GGG 0.467139 3:+81647631 None:intergenic
GAGCTTCTTCTTCAGTGAGC+TGG 0.469415 3:+81650258 None:intergenic
CTTTGCTACCTCAGGTATAG+AGG 0.474305 3:+81649029 None:intergenic
GTCCTCAAATTTCTGGGAAG+AGG 0.478841 3:+81648823 None:intergenic
TCACGCTGCTTTGCTACCTC+AGG 0.479040 3:+81649021 None:intergenic
GCAATGCTGCTGTCTCCACC+AGG 0.480965 3:+81647533 None:intergenic
ATCAGAAGGCTGACGAGAGC+GGG 0.481733 3:+81650307 None:intergenic
CTGACGAGAGCGGGTGGCGG+AGG 0.484128 3:+81650316 None:intergenic
GAATCAACAACAACGGGAGG+AGG 0.485353 3:+81650338 None:intergenic
CAAAGTTCGTGCAGCTGCCA+TGG 0.485787 3:+81648876 None:intergenic
CTTACTTAACAGTTAGAAGA+TGG 0.487759 3:-81647339 MsG0380016296.01.T01:three_prime_UTR
CGCGGTACAATTTCAGGAGG+AGG 0.490011 3:+81648901 None:intergenic
GCGGAGGAATCAACAACAAC+GGG 0.491550 3:+81650332 None:intergenic
CTGTAATTGTAGCCTGCTGG+TGG 0.494796 3:-81647785 MsG0380016296.01.T01:intron
TTCCATGGAGATGTTCCAGC+AGG 0.495508 3:-81647668 MsG0380016296.01.T01:CDS
GCAAAGCTGAGAGAAATAAC+TGG 0.502572 3:-81647620 MsG0380016296.01.T01:CDS
AATGCTGCTGTCTCCACCAG+GGG 0.506313 3:+81647535 None:intergenic
AACCTCTTCCCAGAAATTTG+AGG 0.507551 3:-81648825 MsG0380016296.01.T01:CDS
TGCACGAACTTTGGAATCAA+AGG 0.509149 3:-81648866 MsG0380016296.01.T01:CDS
AAGCTCAATCTCTCTCCAAA+AGG 0.514977 3:-81650241 MsG0380016296.01.T01:intron
GCCAAGACCAAGGAGCTCAC+TGG 0.521289 3:-81648940 MsG0380016296.01.T01:CDS
ATATTACTTTGCCCACCAGC+AGG 0.525761 3:+81647773 None:intergenic
AGGCTGACGAGAGCGGGTGG+CGG 0.529442 3:+81650313 None:intergenic
ATCATTTCCAGTGAGCTCCT+TGG 0.534478 3:+81648933 None:intergenic
TGAAATTGTACCGCGTTCCA+TGG 0.543593 3:-81648893 MsG0380016296.01.T01:CDS
TCAACAACAACGGGAGGAGG+AGG 0.546104 3:+81650341 None:intergenic
ATTCACACTCAAAACAACAA+TGG 0.547288 3:-81650426 MsG0380016296.01.T01:exon
ATCAAAGGAAAGCAAGGACA+TGG 0.548897 3:-81648851 MsG0380016296.01.T01:CDS
GTTTGCGGGCACCTTTAACA+GGG 0.549073 3:+81647558 None:intergenic
GAAGATTCTGCATCAGAAGC+CGG 0.549556 3:-81649297 MsG0380016296.01.T01:CDS
AACTATCATAATTTAGAGCA+AGG 0.555005 3:+81647511 None:intergenic
GCAAAGCAGCGTGAACTGAG+TGG 0.567625 3:-81649012 MsG0380016296.01.T01:CDS
TTGGTGTGTGTCTTTCTCAC+CGG 0.575218 3:+81650395 None:intergenic
ACACTTCCGATCTCCTCACT+TGG 0.577893 3:-81650373 MsG0380016296.01.T01:CDS
TTTGGAATCAAAGGAAAGCA+AGG 0.578160 3:-81648857 MsG0380016296.01.T01:CDS
TCCCAGAAATTTGAGGACAT+CGG 0.579400 3:-81648818 MsG0380016296.01.T01:CDS
ATCCTGCTGGAACATCTCCA+TGG 0.579980 3:+81647666 None:intergenic
GGCAGTGACATATTTGCTGA+TGG 0.581577 3:-81647599 MsG0380016296.01.T01:CDS
GCTACCTCAGGTATAGAGGT+AGG 0.582241 3:+81649033 None:intergenic
ATGAAAGAGATGAGTGGACA+TGG 0.583015 3:-81649339 MsG0380016296.01.T01:CDS
AGTGAGGAGATCGGAAGTGT+TGG 0.589898 3:+81650376 None:intergenic
ATTCCGTGTGAACATTAGTA+GGG 0.590764 3:-81647447 MsG0380016296.01.T01:three_prime_UTR
TTCCGATCTCCTCACTTGGT+CGG 0.594758 3:-81650369 MsG0380016296.01.T01:CDS
CAATGCTGCTGTCTCCACCA+GGG 0.594808 3:+81647534 None:intergenic
TTCCGACCAAGTGAGGAGAT+CGG 0.598387 3:+81650367 None:intergenic
CGTGCAGCTGCCATGGAACG+CGG 0.599947 3:+81648883 None:intergenic
AACAACAATGGGAAGCACTC+CGG 0.604363 3:-81650414 MsG0380016296.01.T01:exon
TATAAAATGAAAGAGATGAG+TGG 0.604599 3:-81649345 MsG0380016296.01.T01:CDS
CAAAGCAGCGTGAACTGAGT+GGG 0.604931 3:-81649011 MsG0380016296.01.T01:CDS
ACCTTGCTGATTCTATCAGA+AGG 0.610704 3:+81650293 None:intergenic
TTTGAGTGTGAATGAATGGA+AGG 0.615001 3:+81650436 None:intergenic
AGAAGGCTGACGAGAGCGGG+TGG 0.616356 3:+81650310 None:intergenic
TATCAGAAGGCTGACGAGAG+CGG 0.623249 3:+81650306 None:intergenic
GAGGAATCAACAACAACGGG+AGG 0.627908 3:+81650335 None:intergenic
GAGGAACTTCCGACCAAGTG+AGG 0.628730 3:+81650360 None:intergenic
ATGCTGCTGTCTCCACCAGG+GGG 0.629578 3:+81647536 None:intergenic
TTACCCTACTAATGTTCACA+CGG 0.631075 3:+81647444 None:intergenic
GCGGTACAATTTCAGGAGGA+GGG 0.631603 3:+81648902 None:intergenic
GCCTTCTGATAGAATCAGCA+AGG 0.638816 3:-81650294 MsG0380016296.01.T01:CDS
TGTAATTGTAGCCTGCTGGT+GGG 0.644942 3:-81647784 MsG0380016296.01.T01:intron
CTTTCAGCAAGCCATGCTCA+GGG 0.644956 3:-81649368 MsG0380016296.01.T01:intron
AATATCAAATGCCAAGACCA+AGG 0.645109 3:-81648950 MsG0380016296.01.T01:CDS
GGCGGAGGAATCAACAACAA+CGG 0.655351 3:+81650331 None:intergenic
TCAAAGGAAAGCAAGGACAT+GGG 0.665351 3:-81648850 MsG0380016296.01.T01:CDS
GAAGCCTACCTCTATACCTG+AGG 0.688793 3:-81649037 MsG0380016296.01.T01:CDS
TGTGAATGAATGGAAGGTGA+AGG 0.689920 3:+81650442 None:intergenic
GAACGCGGTACAATTTCAGG+AGG 0.704954 3:+81648898 None:intergenic
TGGAAGGTGAAGGAACAACG+TGG 0.784347 3:+81650452 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTCAAATTGAAAATAAA+CGG + Chr3:81647851-81647870 None:intergenic 15.0%
!!! ACTCTTCATTTTTATTTTTT+TGG - Chr3:81649313-81649332 MsG0380016296.01.T01:CDS 15.0%
!!! CTCTTCATTTTTATTTTTTT+GGG - Chr3:81649314-81649333 MsG0380016296.01.T01:CDS 15.0%
!!! TAAAATTTAAGTCTGTTTTT+GGG - Chr3:81649623-81649642 MsG0380016296.01.T01:intron 15.0%
!!! TATAATATTTTCTTATGACA+TGG - Chr3:81649145-81649164 MsG0380016296.01.T01:intron 15.0%
!!! TCAAAATCTACTTTTAAATT+TGG - Chr3:81647968-81647987 MsG0380016296.01.T01:intron 15.0%
!!! TCTTCATTTTTATTTTTTTG+GGG - Chr3:81649315-81649334 MsG0380016296.01.T01:CDS 15.0%
!! AAACACATAAAAGAAACAAA+TGG + Chr3:81649860-81649879 None:intergenic 20.0%
!! ATAAAAATCCTACATCAAAA+GGG + Chr3:81650370-81650389 None:intergenic 20.0%
!! TATAAAAATCCTACATCAAA+AGG + Chr3:81650371-81650390 None:intergenic 20.0%
!! TCAATTTGATGTTTGAAAAA+TGG + Chr3:81648656-81648675 None:intergenic 20.0%
!! TCATATTTAAGTGTTTGTTT+AGG + Chr3:81648343-81648362 None:intergenic 20.0%
!!! AAAAAAATCACTTTTTTGTG+TGG + Chr3:81648192-81648211 None:intergenic 20.0%
!!! AGAAAAAAATCTGAAACAAA+CGG - Chr3:81648380-81648399 MsG0380016296.01.T01:intron 20.0%
!!! ATATTTAAATTTTGCATTCC+AGG + Chr3:81649287-81649306 None:intergenic 20.0%
!!! ATCTGTTTCTTAATTTGAAA+TGG - Chr3:81647738-81647757 MsG0380016296.01.T01:CDS 20.0%
!!! ATTAGCTTTTTTCAAATACA+AGG + Chr3:81648256-81648275 None:intergenic 20.0%
!!! ATTCACAATTTTCATAACAA+AGG + Chr3:81648408-81648427 None:intergenic 20.0%
!!! GTAAAATTTAAGTCTGTTTT+TGG - Chr3:81649622-81649641 MsG0380016296.01.T01:intron 20.0%
! AAAAGAAACAAATGGACAAA+TGG + Chr3:81649852-81649871 None:intergenic 25.0%
! AAAGAAACAAATGGACAAAT+GGG + Chr3:81649851-81649870 None:intergenic 25.0%
! AACTCGTAAGTCATAAATAT+GGG + Chr3:81650007-81650026 None:intergenic 25.0%
! AATTAAGAAACAGATCTATG+CGG + Chr3:81647733-81647752 None:intergenic 25.0%
! ATGTTTGAAAAATGGCTATA+AGG + Chr3:81648648-81648667 None:intergenic 25.0%
! GCATATATCAGGTTTATATT+TGG - Chr3:81648590-81648609 MsG0380016296.01.T01:intron 25.0%
! TAAAAATCCTACATCAAAAG+GGG + Chr3:81650369-81650388 None:intergenic 25.0%
! TAACTCGTAAGTCATAAATA+TGG + Chr3:81650008-81650027 None:intergenic 25.0%
! TATAAAATGAAAGAGATGAG+TGG - Chr3:81648493-81648512 MsG0380016296.01.T01:intron 25.0%
! TTCAAACATCAAATTGAACT+CGG - Chr3:81648659-81648678 MsG0380016296.01.T01:intron 25.0%
! TTTAGTTGAATCAAGATTAG+TGG - Chr3:81647895-81647914 MsG0380016296.01.T01:intron 25.0%
!! AAAAAAATCTGAAACAAACG+GGG - Chr3:81648382-81648401 MsG0380016296.01.T01:intron 25.0%
!! AAAACTATTTTCTCACAAAC+TGG + Chr3:81649965-81649984 None:intergenic 25.0%
!! AACTATCATAATTTAGAGCA+AGG + Chr3:81650330-81650349 None:intergenic 25.0%
!! AGTTTTTGAATTAACAGAAC+CGG + Chr3:81648563-81648582 None:intergenic 25.0%
!! GAAAAAAATCTGAAACAAAC+GGG - Chr3:81648381-81648400 MsG0380016296.01.T01:intron 25.0%
!!! ATTTTTATTTTTTTGGGGTG+GGG - Chr3:81649320-81649339 MsG0380016296.01.T01:CDS 25.0%
!!! CATTTTTATTTTTTTGGGGT+GGG - Chr3:81649319-81649338 MsG0380016296.01.T01:CDS 25.0%
!!! CTCTAAAATGAAAAGCTATA+AGG + Chr3:81648279-81648298 None:intergenic 25.0%
!!! GAATTAAAGAAAGTGTTTTC+AGG - Chr3:81647820-81647839 MsG0380016296.01.T01:intron 25.0%
!!! TCATTTTTATTTTTTTGGGG+TGG - Chr3:81649318-81649337 MsG0380016296.01.T01:CDS 25.0%
!!! TGTCTTTGAAATTTCTATGA+TGG - Chr3:81649578-81649597 MsG0380016296.01.T01:intron 25.0%
AAGACAATGTAACAAACTGA+TGG + Chr3:81649564-81649583 None:intergenic 30.0%
AAGATTCAGGTAGTTGTTAA+TGG + Chr3:81649213-81649232 None:intergenic 30.0%
ACTCGTAAGTCATAAATATG+GGG + Chr3:81650006-81650025 None:intergenic 30.0%
ATGTTCAATTATCAGCATCT+AGG - Chr3:81650419-81650438 MsG0380016296.01.T01:exon 30.0%
ATTCACACTCAAAACAACAA+TGG - Chr3:81647412-81647431 MsG0380016296.01.T01:three_prime_UTR 30.0%
CTCACTGGAAATGATATATT+TGG - Chr3:81648913-81648932 MsG0380016296.01.T01:CDS 30.0%
CTTACTTAACAGTTAGAAGA+TGG - Chr3:81650499-81650518 MsG0380016296.01.T01:five_prime_UTR 30.0%
GAAAATTGTGAATCTAAGCT+AGG - Chr3:81648415-81648434 MsG0380016296.01.T01:intron 30.0%
GGGCAAAGTAATATCTTATT+TGG - Chr3:81650074-81650093 MsG0380016296.01.T01:intron 30.0%
GTAAAAGATCTGATCTGAAA+AGG + Chr3:81647649-81647668 None:intergenic 30.0%
TAAGATGTGTGTGTTATGTT+TGG - Chr3:81649692-81649711 MsG0380016296.01.T01:intron 30.0%
TTATCGATATGAATTGAGCT+AGG - Chr3:81649168-81649187 MsG0380016296.01.T01:intron 30.0%
TTCACACTCAAAACAACAAT+GGG - Chr3:81647413-81647432 MsG0380016296.01.T01:three_prime_UTR 30.0%
TTCTAATGTAAGTGTCTTCT+AGG - Chr3:81649050-81649069 MsG0380016296.01.T01:CDS 30.0%
! ATTTGGCAAAAGCTACATTT+TGG + Chr3:81649770-81649789 None:intergenic 30.0%
! TCAGGCAATAATATTTTCCA+TGG - Chr3:81650155-81650174 MsG0380016296.01.T01:intron 30.0%
!! AAATGAAAGTACGTACCTTT+TGG + Chr3:81647615-81647634 None:intergenic 30.0%
!! TGGCTTCAATTTAATTTGAC+AGG + Chr3:81649513-81649532 None:intergenic 30.0%
!!! AGCTTTTTTCAAATACAAGG+AGG + Chr3:81648253-81648272 None:intergenic 30.0%
!!! TTGTTTTGAGTGTGAATGAA+TGG + Chr3:81647409-81647428 None:intergenic 30.0%
!!! TTTTTATTTTTTTGGGGTGG+GGG - Chr3:81649321-81649340 MsG0380016296.01.T01:CDS 30.0%
AACAACTACCTGAATCTTAG+TGG - Chr3:81649215-81649234 MsG0380016296.01.T01:intron 35.0%
AACTAGCATACGCATATATC+AGG - Chr3:81648579-81648598 MsG0380016296.01.T01:intron 35.0%
AAGATCTGATCTGAAAAGGA+AGG + Chr3:81647645-81647664 None:intergenic 35.0%
AATATCAAATGCCAAGACCA+AGG - Chr3:81648888-81648907 MsG0380016296.01.T01:CDS 35.0%
AATTCCGTGTGAACATTAGT+AGG - Chr3:81650390-81650409 MsG0380016296.01.T01:CDS 35.0%
ACACATTCACGTAGTTAACA+TGG + Chr3:81648023-81648042 None:intergenic 35.0%
ATAAGCAGTAGGTACTTTGT+AGG + Chr3:81649909-81649928 None:intergenic 35.0%
ATGCAATCTGCAATCTTATG+TGG - Chr3:81647872-81647891 MsG0380016296.01.T01:intron 35.0%
ATTCCGTGTGAACATTAGTA+GGG - Chr3:81650391-81650410 MsG0380016296.01.T01:CDS 35.0%
CTACAATTACAGGAAAAGGA+AGG + Chr3:81650045-81650064 None:intergenic 35.0%
CTTAGGACTAACACTTTCTT+CGG + Chr3:81648782-81648801 None:intergenic 35.0%
GAAATCAAAGACCCTGTTAA+AGG - Chr3:81650269-81650288 MsG0380016296.01.T01:CDS 35.0%
GAGATTGACGAAATCACAAT+TGG + Chr3:81649802-81649821 None:intergenic 35.0%
GATCGAAGAACGATCAAAAA+AGG + Chr3:81647685-81647704 None:intergenic 35.0%
GTGTGTTATGTTTGGATTGA+CGG - Chr3:81649700-81649719 MsG0380016296.01.T01:intron 35.0%
TTACCCTACTAATGTTCACA+CGG + Chr3:81650397-81650416 None:intergenic 35.0%
TTCTTTGATGTCTTCTCAAC+AGG + Chr3:81650107-81650126 None:intergenic 35.0%
TTTGAGTGTGAATGAATGGA+AGG + Chr3:81647405-81647424 None:intergenic 35.0%
! CATGGTATATTTTCGGACAA+TGG - Chr3:81648517-81648536 MsG0380016296.01.T01:intron 35.0%
! CTATTTTCTCACAAACTGGA+CGG + Chr3:81649961-81649980 None:intergenic 35.0%
! GAGTGGACATGGTATATTTT+CGG - Chr3:81648510-81648529 MsG0380016296.01.T01:intron 35.0%
! TGTAGCTTTTGCCAAATTCT+TGG - Chr3:81649773-81649792 MsG0380016296.01.T01:intron 35.0%
! TTAACAGGGTCTTTGATTTC+TGG + Chr3:81650269-81650288 None:intergenic 35.0%
! TTCATTTTATACCCTGAGCA+TGG + Chr3:81648484-81648503 None:intergenic 35.0%
! TTTGGAATCAAAGGAAAGCA+AGG - Chr3:81648981-81649000 MsG0380016296.01.T01:CDS 35.0%
!!! AAGATTTTAGTTTGGGGGTT+GGG - Chr3:81649353-81649372 MsG0380016296.01.T01:CDS 35.0%
!!! AGGGGGTAAGATTTTAGTTT+GGG - Chr3:81649346-81649365 MsG0380016296.01.T01:CDS 35.0%
!!! TAAGATTTTAGTTTGGGGGT+TGG - Chr3:81649352-81649371 MsG0380016296.01.T01:CDS 35.0%
!!! TAAGTCTGTTTTTGGGTGTT+GGG - Chr3:81649630-81649649 MsG0380016296.01.T01:intron 35.0%
!!! TAGGGGGTAAGATTTTAGTT+TGG - Chr3:81649345-81649364 MsG0380016296.01.T01:CDS 35.0%
!!! TTAAGTCTGTTTTTGGGTGT+TGG - Chr3:81649629-81649648 MsG0380016296.01.T01:intron 35.0%
AACCTCTTCCCAGAAATTTG+AGG - Chr3:81649013-81649032 MsG0380016296.01.T01:CDS 40.0%
AAGCTCAATCTCTCTCCAAA+AGG - Chr3:81647597-81647616 MsG0380016296.01.T01:CDS 40.0%
AATGGGACTGAGAACTTGAT+TGG + Chr3:81649834-81649853 None:intergenic 40.0%
ACAATTGGCCACCAAGAATT+TGG + Chr3:81649787-81649806 None:intergenic 40.0%
ACCGATGTCCTCAAATTTCT+GGG + Chr3:81649024-81649043 None:intergenic 40.0%
ATCAAAGGAAAGCAAGGACA+TGG - Chr3:81648987-81649006 MsG0380016296.01.T01:CDS 40.0%
ATCGTTGGATTGAGATCAGA+CGG + Chr3:81647995-81648014 None:intergenic 40.0%
ATGAAAGAGATGAGTGGACA+TGG - Chr3:81648499-81648518 MsG0380016296.01.T01:intron 40.0%
ATGGCTATAAGGTCTCAAAC+TGG + Chr3:81648637-81648656 None:intergenic 40.0%
ATTCATCGCCACTAAGATTC+AGG + Chr3:81649226-81649245 None:intergenic 40.0%
CAGGCTACAATTACAGGAAA+AGG + Chr3:81650049-81650068 None:intergenic 40.0%
CAGTTTACCTTATGCTTTCC+TGG - Chr3:81649266-81649285 MsG0380016296.01.T01:CDS 40.0%
CGGTGAGTTTGGTAAAATCA+CGG - Chr3:81649720-81649739 MsG0380016296.01.T01:intron 40.0%
GCAAAGCTGAGAGAAATAAC+TGG - Chr3:81650218-81650237 MsG0380016296.01.T01:intron 40.0%
GCATCTCAGCAAGCAATAAA+TGG - Chr3:81648733-81648752 MsG0380016296.01.T01:intron 40.0%
GTGTAAGATGTCCACAAGAT+TGG + Chr3:81649453-81649472 None:intergenic 40.0%
GTTAACATGGATGTGATCGT+TGG + Chr3:81648010-81648029 None:intergenic 40.0%
TAATACTGTTGCAGCACATG+AGG - Chr3:81649426-81649445 MsG0380016296.01.T01:intron 40.0%
TCAAACTGGACTCAAGATCA+AGG + Chr3:81648623-81648642 None:intergenic 40.0%
TCAAAGGAAAGCAAGGACAT+GGG - Chr3:81648988-81649007 MsG0380016296.01.T01:CDS 40.0%
TCCCAGAAATTTGAGGACAT+CGG - Chr3:81649020-81649039 MsG0380016296.01.T01:CDS 40.0%
TGTCGAGCTAAATAAGCAGT+AGG + Chr3:81649920-81649939 None:intergenic 40.0%
TGTGAATCTAAGCTAGGAGA+TGG - Chr3:81648421-81648440 MsG0380016296.01.T01:intron 40.0%
TGTGAATGAATGGAAGGTGA+AGG + Chr3:81647399-81647418 None:intergenic 40.0%
! AAGCTTACCCCTTTTGATGT+AGG - Chr3:81650359-81650378 MsG0380016296.01.T01:CDS 40.0%
! AGCTTTTGCCAAATTCTTGG+TGG - Chr3:81649776-81649795 MsG0380016296.01.T01:intron 40.0%
! CAGGAAAGCATAAGGTAAAC+TGG + Chr3:81649268-81649287 None:intergenic 40.0%
! GAAAGCATAAGGTAAACTGG+TGG + Chr3:81649265-81649284 None:intergenic 40.0%
! TAACAAACTGATGGACCTTC+CGG + Chr3:81649555-81649574 None:intergenic 40.0%
! TTGCATTCCAGGAAAGCATA+AGG + Chr3:81649276-81649295 None:intergenic 40.0%
!! ACCTTGCTGATTCTATCAGA+AGG + Chr3:81647548-81647567 None:intergenic 40.0%
!! TGCACGAACTTTGGAATCAA+AGG - Chr3:81648972-81648991 MsG0380016296.01.T01:CDS 40.0%
!!! AAGTCTGTTTTTGGGTGTTG+GGG - Chr3:81649631-81649650 MsG0380016296.01.T01:intron 40.0%
!!! AGATTTTAGTTTGGGGGTTG+GGG - Chr3:81649354-81649373 MsG0380016296.01.T01:CDS 40.0%
!!! GGGGGTAAGATTTTAGTTTG+GGG - Chr3:81649347-81649366 MsG0380016296.01.T01:CDS 40.0%
!!! GGGGTAAGATTTTAGTTTGG+GGG - Chr3:81649348-81649367 MsG0380016296.01.T01:CDS 40.0%
AACTGCACATGCATTGCATC+CGG - Chr3:81649533-81649552 MsG0380016296.01.T01:intron 45.0%
AGCTGAAAAATCCCTGAGCA+GGG - Chr3:81650196-81650215 MsG0380016296.01.T01:intron 45.0%
ATATTACTTTGCCCACCAGC+AGG + Chr3:81650068-81650087 None:intergenic 45.0%
ATCATTTCCAGTGAGCTCCT+TGG + Chr3:81648908-81648927 None:intergenic 45.0%
ATGGAACGCGGTACAATTTC+AGG + Chr3:81648946-81648965 None:intergenic 45.0%
CACCGATGTCCTCAAATTTC+TGG + Chr3:81649025-81649044 None:intergenic 45.0%
CAGAAGTTTGCAGAGCTTTC+AGG - Chr3:81650137-81650156 MsG0380016296.01.T01:intron 45.0%
CAGGAAAAGGAAGGTTAACC+TGG + Chr3:81650036-81650055 None:intergenic 45.0%
CTCACAAACTGGACGGTTTA+AGG + Chr3:81649954-81649973 None:intergenic 45.0%
CTGAAAGCTCTGCAAACTTC+TGG + Chr3:81650139-81650158 None:intergenic 45.0%
CTTTGCTACCTCAGGTATAG+AGG + Chr3:81648812-81648831 None:intergenic 45.0%
GAAGATTCTGCATCAGAAGC+CGG - Chr3:81648541-81648560 MsG0380016296.01.T01:intron 45.0%
GACAGGCACATGTTAAGAGA+AGG + Chr3:81649496-81649515 None:intergenic 45.0%
GATGCAATGCATGTGCAGTT+TGG + Chr3:81649533-81649552 None:intergenic 45.0%
GCATAAGGTAAACTGGTGGA+TGG + Chr3:81649261-81649280 None:intergenic 45.0%
GCCTTCTGATAGAATCAGCA+AGG - Chr3:81647544-81647563 MsG0380016296.01.T01:CDS 45.0%
GGTATAGAGGTAGGCTTCTT+AGG + Chr3:81648799-81648818 None:intergenic 45.0%
GTTTGGATTGACGGTGAGTT+TGG - Chr3:81649709-81649728 MsG0380016296.01.T01:intron 45.0%
TGAAATTGTACCGCGTTCCA+TGG - Chr3:81648945-81648964 MsG0380016296.01.T01:CDS 45.0%
TGGGACTTTGCAAACTGACT+TGG - Chr3:81648846-81648865 MsG0380016296.01.T01:CDS 45.0%
TGTAATTGTAGCCTGCTGGT+GGG - Chr3:81650054-81650073 MsG0380016296.01.T01:intron 45.0%
TTCCTGTAATTGTAGCCTGC+TGG - Chr3:81650050-81650069 MsG0380016296.01.T01:intron 45.0%
TTGGTGTGTGTCTTTCTCAC+CGG + Chr3:81647446-81647465 None:intergenic 45.0%
! GGCAGTGACATATTTGCTGA+TGG - Chr3:81650239-81650258 MsG0380016296.01.T01:intron 45.0%
! GTAAACTGGTGGATGGTCAA+GGG + Chr3:81649254-81649273 None:intergenic 45.0%
! GTCCTCAAATTTCTGGGAAG+AGG + Chr3:81649018-81649037 None:intergenic 45.0%
!! AACAACAATGGGAAGCACTC+CGG - Chr3:81647424-81647443 MsG0380016296.01.T01:three_prime_UTR 45.0%
!! GATTTTTCAGCTGATCCTGC+TGG + Chr3:81650188-81650207 None:intergenic 45.0%
!!! AGTCTGTTTTTGGGTGTTGG+GGG - Chr3:81649632-81649651 MsG0380016296.01.T01:intron 45.0%
!!! ATTTTAGTTTGGGGGTTGGG+GGG - Chr3:81649356-81649375 MsG0380016296.01.T01:CDS 45.0%
!!! ATTTTTTTGGGGTGGGGGTT+AGG - Chr3:81649326-81649345 MsG0380016296.01.T01:CDS 45.0%
!!! GATTTTAGTTTGGGGGTTGG+GGG - Chr3:81649355-81649374 MsG0380016296.01.T01:CDS 45.0%
!!! TTTTTTTGGGGTGGGGGTTA+GGG - Chr3:81649327-81649346 MsG0380016296.01.T01:CDS 45.0%
ACACTTCCGATCTCCTCACT+TGG - Chr3:81647465-81647484 MsG0380016296.01.T01:three_prime_UTR 50.0%
ACTTTCAGCAAGCCATGCTC+AGG - Chr3:81648469-81648488 MsG0380016296.01.T01:intron 50.0%
ATCCTGCTGGAACATCTCCA+TGG + Chr3:81650175-81650194 None:intergenic 50.0%
CAAAGCAGCGTGAACTGAGT+GGG - Chr3:81648827-81648846 MsG0380016296.01.T01:CDS 50.0%
CACCAGCAGGCTACAATTAC+AGG + Chr3:81650055-81650074 None:intergenic 50.0%
CAGCTGAAAAATCCCTGAGC+AGG - Chr3:81650195-81650214 MsG0380016296.01.T01:intron 50.0%
CTGTAATTGTAGCCTGCTGG+TGG - Chr3:81650053-81650072 MsG0380016296.01.T01:intron 50.0%
CTTTCAGCAAGCCATGCTCA+GGG - Chr3:81648470-81648489 MsG0380016296.01.T01:intron 50.0%
GAACGCGGTACAATTTCAGG+AGG + Chr3:81648943-81648962 None:intergenic 50.0%
GAAGCCTACCTCTATACCTG+AGG - Chr3:81648801-81648820 MsG0380016296.01.T01:intron 50.0%
GAATCAACAACAACGGGAGG+AGG + Chr3:81647503-81647522 None:intergenic 50.0%
GACTTACGAGTTACGACTCC+AGG - Chr3:81650015-81650034 MsG0380016296.01.T01:intron 50.0%
GAGCTTCTTCTTCAGTGAGC+TGG + Chr3:81647583-81647602 None:intergenic 50.0%
GAGGAATCAACAACAACGGG+AGG + Chr3:81647506-81647525 None:intergenic 50.0%
GCACATGAGGACCAATCTTG+TGG - Chr3:81649439-81649458 MsG0380016296.01.T01:intron 50.0%
GCGGAGGAATCAACAACAAC+GGG + Chr3:81647509-81647528 None:intergenic 50.0%
GCGGTACAATTTCAGGAGGA+GGG + Chr3:81648939-81648958 None:intergenic 50.0%
GCTACCTCAGGTATAGAGGT+AGG + Chr3:81648808-81648827 None:intergenic 50.0%
GGCGGAGGAATCAACAACAA+CGG + Chr3:81647510-81647529 None:intergenic 50.0%
GTTTGCGGGCACCTTTAACA+GGG + Chr3:81650283-81650302 None:intergenic 50.0%
TATCAGAAGGCTGACGAGAG+CGG + Chr3:81647535-81647554 None:intergenic 50.0%
TGGAAGGTGAAGGAACAACG+TGG + Chr3:81647389-81647408 None:intergenic 50.0%
TTCCATGGAGATGTTCCAGC+AGG - Chr3:81650170-81650189 MsG0380016296.01.T01:intron 50.0%
TTCCGACCAAGTGAGGAGAT+CGG + Chr3:81647474-81647493 None:intergenic 50.0%
TTCCGATCTCCTCACTTGGT+CGG - Chr3:81647469-81647488 MsG0380016296.01.T01:three_prime_UTR 50.0%
! AGTGAGGAGATCGGAAGTGT+TGG + Chr3:81647465-81647484 None:intergenic 50.0%
! GGTAAACTGGTGGATGGTCA+AGG + Chr3:81649255-81649274 None:intergenic 50.0%
!!! TGTTTTTGGGTGTTGGGGGT+GGG - Chr3:81649636-81649655 MsG0380016296.01.T01:intron 50.0%
!!! TTTTTTGGGGTGGGGGTTAG+GGG - Chr3:81649328-81649347 MsG0380016296.01.T01:CDS 50.0%
AATGCTGCTGTCTCCACCAG+GGG + Chr3:81650306-81650325 None:intergenic 55.0%
ATCAGAAGGCTGACGAGAGC+GGG + Chr3:81647534-81647553 None:intergenic 55.0%
CAAAGTTCGTGCAGCTGCCA+TGG + Chr3:81648965-81648984 None:intergenic 55.0%
CAATGCTGCTGTCTCCACCA+GGG + Chr3:81650307-81650326 None:intergenic 55.0%
CATGGCAGCTGCACGAACTT+TGG - Chr3:81648963-81648982 MsG0380016296.01.T01:CDS 55.0%
CGCGGTACAATTTCAGGAGG+AGG + Chr3:81648940-81648959 None:intergenic 55.0%
GAGGAACTTCCGACCAAGTG+AGG + Chr3:81647481-81647500 None:intergenic 55.0%
GCAAAGCAGCGTGAACTGAG+TGG - Chr3:81648826-81648845 MsG0380016296.01.T01:CDS 55.0%
GCACATGCATTGCATCCGGA+AGG - Chr3:81649537-81649556 MsG0380016296.01.T01:intron 55.0%
GGTTTGCGGGCACCTTTAAC+AGG + Chr3:81650284-81650303 None:intergenic 55.0%
TCAACAACAACGGGAGGAGG+AGG + Chr3:81647500-81647519 None:intergenic 55.0%
TCCAGTGAGCTCCTTGGTCT+TGG + Chr3:81648902-81648921 None:intergenic 55.0%
TCTCAGCTTTGCCCTGCTCA+GGG + Chr3:81650210-81650229 None:intergenic 55.0%
! TCACGCTGCTTTGCTACCTC+AGG + Chr3:81648820-81648839 None:intergenic 55.0%
!!! CTGTTTTTGGGTGTTGGGGG+TGG - Chr3:81649635-81649654 MsG0380016296.01.T01:intron 55.0%
!!! GTTTTTGGGTGTTGGGGGTG+GGG - Chr3:81649637-81649656 MsG0380016296.01.T01:intron 55.0%
!!! TTTTTGGGGTGGGGGTTAGG+GGG - Chr3:81649329-81649348 MsG0380016296.01.T01:CDS 55.0%
ATGCTGCTGTCTCCACCAGG+GGG + Chr3:81650305-81650324 None:intergenic 60.0%
CTCTCAGCTTTGCCCTGCTC+AGG + Chr3:81650211-81650230 None:intergenic 60.0%
GCAATGCTGCTGTCTCCACC+AGG + Chr3:81650308-81650327 None:intergenic 60.0%
GCCAAGACCAAGGAGCTCAC+TGG - Chr3:81648898-81648917 MsG0380016296.01.T01:CDS 60.0%
TGTCTCCACCAGGGGGTTTG+CGG + Chr3:81650298-81650317 None:intergenic 60.0%
AGAAGGCTGACGAGAGCGGG+TGG + Chr3:81647531-81647550 None:intergenic 65.0%
CGTGCAGCTGCCATGGAACG+CGG + Chr3:81648958-81648977 None:intergenic 65.0%
GTCTCCACCAGGGGGTTTGC+GGG + Chr3:81650297-81650316 None:intergenic 65.0%
!! AAAGGTGCCCGCAAACCCCC+TGG - Chr3:81650287-81650306 MsG0380016296.01.T01:CDS 65.0%
AGGCTGACGAGAGCGGGTGG+CGG + Chr3:81647528-81647547 None:intergenic 70.0%
GGTGCCCGCAAACCCCCTGG+TGG - Chr3:81650290-81650309 MsG0380016296.01.T01:CDS 75.0%
! CTGACGAGAGCGGGTGGCGG+AGG + Chr3:81647525-81647544 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr3 gene 81647332 81650528 81647332 ID=MsG0380016296.01;Name=MsG0380016296.01
Chr3 mRNA 81647332 81650528 81647332 ID=MsG0380016296.01.T01;Parent=MsG0380016296.01;Name=MsG0380016296.01.T01;_AED=0.32;_eAED=0.32;_QI=99|1|1|1|1|1|4|191|299
Chr3 exon 81647332 81647795 81647332 ID=MsG0380016296.01.T01:exon:33051;Parent=MsG0380016296.01.T01
Chr3 exon 81648804 81649118 81648804 ID=MsG0380016296.01.T01:exon:33050;Parent=MsG0380016296.01.T01
Chr3 exon 81649260 81649383 81649260 ID=MsG0380016296.01.T01:exon:33049;Parent=MsG0380016296.01.T01
Chr3 exon 81650242 81650528 81650242 ID=MsG0380016296.01.T01:exon:33048;Parent=MsG0380016296.01.T01
Chr3 five_prime_UTR 81650430 81650528 81650430 ID=MsG0380016296.01.T01:five_prime_utr;Parent=MsG0380016296.01.T01
Chr3 CDS 81650242 81650429 81650242 ID=MsG0380016296.01.T01:cds;Parent=MsG0380016296.01.T01
Chr3 CDS 81649260 81649383 81649260 ID=MsG0380016296.01.T01:cds;Parent=MsG0380016296.01.T01
Chr3 CDS 81648804 81649118 81648804 ID=MsG0380016296.01.T01:cds;Parent=MsG0380016296.01.T01
Chr3 CDS 81647523 81647795 81647523 ID=MsG0380016296.01.T01:cds;Parent=MsG0380016296.01.T01
Chr3 three_prime_UTR 81647332 81647522 81647332 ID=MsG0380016296.01.T01:three_prime_utr;Parent=MsG0380016296.01.T01
Gene Sequence

>MsG0380016296.01.T01

ATGGGAAGCACTCCGGTGAGAAAGACACACACCAACACTTCCGATCTCCTCACTTGGTCGGAAGTTCCTCCTCCTCCCGTTGTTGTTGATTCCTCCGCCACCCGCTCTCGTCAGCCTTCTGATAGAATCAGCAAGGTTCTCAATAGTGACCAGCTCACTGAAGAAGAAGCTCAATCTCTCTCCAAAAGCAAGCCATGCTCAGGGTATAAAATGAAAGAGATGAGTGGACATGGTATATTTTCGGACAATGGTGAAGATTCTGCATCAGAAGCCGGTTCTGTTAATTCAAAAACTAGCATACGCATATATCAGCAAGCAATAAATGGAGTTAGTCAGATATCATTCAGCACCGAAGAAAGTGTTAGTCCTAAGAAGCCTACCTCTATACCTGAGGTAGCAAAGCAGCGTGAACTGAGTGGGACTTTGCAAACTGACTTGGACGCAAAGACTCAGAAGCAAATATCAAATGCCAAGACCAAGGAGCTCACTGGAAATGATATATTTGGCCCTCCTCCTGAAATTGTACCGCGTTCCATGGCAGCTGCACGAACTTTGGAATCAAAGGAAAGCAAGGACATGGGAGAACCTCTTCCCAGAAATTTGAGGACATCGGTGAAAGTTTCTAATCCTGCTGGTGGGCAAAGTAATATCTTATTTGGCGACGCACCTGTTGAGAAGACATCAAAGAAGATACATGACCAGAAGTTTGCAGAGCTTTCAGGCAATAATATTTTCCATGGAGATGTTCCAGCAGGATCAGCTGAAAAATCCCTGAGCAGGGCAAAGCTGAGAGAAATAACTGGCAGTGACATATTTGCTGATGGAAAACCAGAAATCAAAGACCCTGTTAAAGGTGCCCGCAAACCCCCTGGTGGAGACAGCAGCATTGCCTTGCTCTAA
Protein sequence

>MsG0380016296.01.T01

MGSTPVRKTHTNTSDLLTWSEVPPPPVVVDSSATRSRQPSDRISKVLNSDQLTEEEAQSLSKSKPCSGYKMKEMSGHGIFSDNGEDSASEAGSVNSKTSIRIYQQAINGVSQISFSTEESVSPKKPTSIPEVAKQRELSGTLQTDLDAKTQKQISNAKTKELTGNDIFGPPPEIVPRSMAAARTLESKESKDMGEPLPRNLRTSVKVSNPAGGQSNILFGDAPVEKTSKKIHDQKFAELSGNNIFHGDVPAGSAEKSLSRAKLREITGSDIFADGKPEIKDPVKGARKPPGGDSSIALL*