AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006183.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006183.01.T01 MTR_1g115475 63.000 200 1 2 19 145 279 478 1.44e-75 234
MsG0180006183.01.T01 MTR_7g023060 49.246 199 28 2 20 145 302 500 2.70e-56 184
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006183.01.T01 AT3G55260 46.734 199 33 2 20 145 285 483 2.84e-50 168
MsG0180006183.01.T01 AT1G65590 37.688 199 51 3 20 145 282 480 1.07e-34 126
MsG0180006183.01.T01 AT1G05590 33.019 106 47 2 64 145 413 518 3.99e-11 60.1
MsG0180006183.01.T01 AT1G05590 33.019 106 47 2 64 145 413 518 3.99e-11 60.1
MsG0180006183.01.T01 AT1G05590 33.019 106 47 2 64 145 413 518 3.99e-11 60.1

Find 39 sgRNAs with CRISPR-Local

Find 121 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACCTTTGATGTCCTTTC+TGG 0.128238 1:+101074712 MsG0180006183.01.T01:CDS
GGGTTGGATATCCTGATCTT+TGG 0.342390 1:+101074647 MsG0180006183.01.T01:CDS
TCTAACACTCACTCTCTTGT+TGG 0.352802 1:-101074453 None:intergenic
TGCCCGAAGGTTGTTGCAAA+AGG 0.416967 1:+101075882 MsG0180006183.01.T01:CDS
CAGATGTTCAGCAAACAATA+TGG 0.417087 1:+101076072 MsG0180006183.01.T01:CDS
GTCCTTTCTGGCATTATGAC+AGG 0.436989 1:+101074724 MsG0180006183.01.T01:CDS
TTTGTTCACCTGAGTAGTAT+TGG 0.441712 1:-101075239 None:intergenic
TAAATGGGTCCCACGTATAG+TGG 0.462213 1:+101074409 None:intergenic
TATAATGCTGAGCCACTAGA+AGG 0.465489 1:+101075975 MsG0180006183.01.T01:CDS
CCTCGCGCTGCAGCAGCAGC+AGG 0.473773 1:+101076095 MsG0180006183.01.T01:CDS
TGACCATGTGGATGTACCTT+GGG 0.487183 1:+101075944 MsG0180006183.01.T01:CDS
ATACTACTCAGGTGAACAAA+TGG 0.488009 1:+101075242 MsG0180006183.01.T01:CDS
GTGGGCGATGGCCAAAGATC+AGG 0.502649 1:-101074658 None:intergenic
CCTGCTGCTGCTGCAGCGCG+AGG 0.521725 1:-101076095 None:intergenic
GGGACCTGGTGTTTGCCCGA+AGG 0.541881 1:+101075869 MsG0180006183.01.T01:CDS
TTGACCATGTGGATGTACCT+TGG 0.557187 1:+101075943 MsG0180006183.01.T01:CDS
TTGTTGGATGAAATCAATGC+GGG 0.562142 1:-101074437 None:intergenic
ATCTAGCGGGCTCCTACACG+TGG 0.564253 1:-101074677 None:intergenic
AACCTGTCATAATGCCAGAA+AGG 0.564978 1:-101074726 None:intergenic
GGGCTCCTACACGTGGGCGA+TGG 0.575538 1:-101074670 None:intergenic
ACTTCTTTGAAACATCTAGC+GGG 0.579209 1:-101074690 None:intergenic
ACAACCTTCGGGCAAACACC+AGG 0.584654 1:-101075873 None:intergenic
TTGGAGGAGAAGTTTGCATG+TGG 0.586395 1:+101076030 MsG0180006183.01.T01:CDS
TTTGGCCATCGCCCACGTGT+AGG 0.588671 1:+101074665 MsG0180006183.01.T01:CDS
GCTTCATGTATTCCTTCTAG+TGG 0.596470 1:-101075987 None:intergenic
AGGTTGTTGCAAAAGGCTTG+AGG 0.602167 1:+101075889 MsG0180006183.01.T01:CDS
AATGCCAGAAAGGACATCAA+AGG 0.612911 1:-101074716 None:intergenic
TTGCTGGACCAATACTACTC+AGG 0.616269 1:+101075231 MsG0180006183.01.T01:CDS
AACTTCTTTGAAACATCTAG+CGG 0.621539 1:-101074691 None:intergenic
TGGAGGAGAAGTTTGCATGT+GGG 0.624933 1:+101076031 MsG0180006183.01.T01:CDS
GCGGGTCTTACCACTATACG+TGG 0.633542 1:-101074419 None:intergenic
GGTCAAGATACCATACACCT+TGG 0.642283 1:-101075926 None:intergenic
AATTACCGTAGGTTGGGACC+TGG 0.647597 1:+101075855 MsG0180006183.01.T01:intron
GCATTATAGACAACATCCCA+AGG 0.659122 1:-101075960 None:intergenic
CATCCCAAGGTACATCCACA+TGG 0.659431 1:-101075947 None:intergenic
CTTGTTGGATGAAATCAATG+CGG 0.699759 1:-101074438 None:intergenic
TGTATGGTATCTTGACCATG+TGG 0.719486 1:+101075932 MsG0180006183.01.T01:CDS
CGGGTCTTACCACTATACGT+GGG 0.730340 1:-101074418 None:intergenic
TCTAGCGGGCTCCTACACGT+GGG 0.741821 1:-101074676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCATAATAAAAAATAGAAAT+TGG + Chr1:101075136-101075155 MsG0180006183.01.T01:intron 10.0%
!! AAAGTTGGATTTAAAAAATA+TGG - Chr1:101075688-101075707 None:intergenic 15.0%
!! AATATCTGTAAATTTAGAAA+CGG - Chr1:101074880-101074899 None:intergenic 15.0%
!! CTAATAATAAAATGATTCTT+AGG - Chr1:101075033-101075052 None:intergenic 15.0%
!!! AACTTTTTTATGTAACTTAT+CGG + Chr1:101075702-101075721 MsG0180006183.01.T01:intron 15.0%
!!! TTATATTTTGTTTCTGTTTA+GGG + Chr1:101074626-101074645 MsG0180006183.01.T01:intron 15.0%
!! ATTATTAGTACAACTTAAGT+TGG + Chr1:101075045-101075064 MsG0180006183.01.T01:intron 20.0%
!! GATAAGTTACATAAAAAAGT+TGG - Chr1:101075703-101075722 None:intergenic 20.0%
!! GTACATATTTCTTTCAATAA+AGG + Chr1:101074521-101074540 MsG0180006183.01.T01:intron 20.0%
!! TATAACAACCTTAATATTTC+TGG - Chr1:101074611-101074630 None:intergenic 20.0%
!!! ACTTTTTTATGTAACTTATC+GGG + Chr1:101075703-101075722 MsG0180006183.01.T01:intron 20.0%
!!! GTTATATTTTGTTTCTGTTT+AGG + Chr1:101074625-101074644 MsG0180006183.01.T01:intron 20.0%
!!! TATATTTTGTTTCTGTTTAG+GGG + Chr1:101074627-101074646 MsG0180006183.01.T01:intron 20.0%
! AAGAACATGAAATAGTAAAC+TGG - Chr1:101075657-101075676 None:intergenic 25.0%
! ATTGTACACCAGAAATATTA+AGG + Chr1:101074600-101074619 MsG0180006183.01.T01:intron 25.0%
! CTGTCAGTAGAAATAATAAT+TGG - Chr1:101074575-101074594 None:intergenic 25.0%
! TAAAATGATTCTTAGGACTT+AGG - Chr1:101075026-101075045 None:intergenic 25.0%
! TTTAACGAATTTACAGTTCT+AGG - Chr1:101075330-101075349 None:intergenic 25.0%
!! TACAATGATGCATGTTTTAT+AGG - Chr1:101074757-101074776 None:intergenic 25.0%
!!! TTTTTTTTCTTTGCCATATC+CGG + Chr1:101075420-101075439 MsG0180006183.01.T01:intron 25.0%
AAAAACCACATTCTATGTTG+AGG - Chr1:101075395-101075414 None:intergenic 30.0%
ATAAACCTCAACATAGAATG+TGG + Chr1:101075387-101075406 MsG0180006183.01.T01:intron 30.0%
ATTCTTAGGACTTAGGTTAA+TGG - Chr1:101075019-101075038 None:intergenic 30.0%
CTTAAAGATTTCATGTCAAG+TGG - Chr1:101074996-101075015 None:intergenic 30.0%
CTTACCAATACTTTGTATGA+AGG + Chr1:101075572-101075591 MsG0180006183.01.T01:intron 30.0%
GTAAGTTACAGAAAAAGACA+AGG - Chr1:101074821-101074840 None:intergenic 30.0%
GTACGACAATAATCAATTCA+TGG - Chr1:101075479-101075498 None:intergenic 30.0%
GTATTTCATGTAATTACCGT+AGG + Chr1:101075844-101075863 MsG0180006183.01.T01:intron 30.0%
TCATTTCCATTGATATGAAG+TGG - Chr1:101075110-101075129 None:intergenic 30.0%
TTTCATCCATGTAGTTCATT+TGG + Chr1:101075786-101075805 MsG0180006183.01.T01:intron 30.0%
! TTTTCTGTAACTTTAACCAC+CGG - Chr1:101075442-101075461 None:intergenic 30.0%
!! AACTTCTTTGAAACATCTAG+CGG - Chr1:101074694-101074713 None:intergenic 30.0%
!! ATGAAATGAATGAGTATCGA+AGG - Chr1:101075280-101075299 None:intergenic 30.0%
!!! ACTTTATTTTGACAGATTGC+TGG + Chr1:101075215-101075234 MsG0180006183.01.T01:intron 30.0%
ACCCAAGTGAAATAGTTCAA+AGG - Chr1:101074914-101074933 None:intergenic 35.0%
ATACTACTCAGGTGAACAAA+TGG + Chr1:101075242-101075261 MsG0180006183.01.T01:CDS 35.0%
CAGATGTTCAGCAAACAATA+TGG + Chr1:101076072-101076091 MsG0180006183.01.T01:CDS 35.0%
CCCAAGTGAAATAGTTCAAA+GGG - Chr1:101074913-101074932 None:intergenic 35.0%
CCCTTTGAACTATTTCACTT+GGG + Chr1:101074910-101074929 MsG0180006183.01.T01:intron 35.0%
CTTGTTGGATGAAATCAATG+CGG - Chr1:101074441-101074460 None:intergenic 35.0%
GAAATCACCATTATGCACAA+CGG - Chr1:101075770-101075789 None:intergenic 35.0%
GTAAAGATCATACCATTGAC+AGG - Chr1:101075630-101075649 None:intergenic 35.0%
TCATGTAATTACCGTAGGTT+GGG + Chr1:101075849-101075868 MsG0180006183.01.T01:intron 35.0%
TCCCTTTGAACTATTTCACT+TGG + Chr1:101074909-101074928 MsG0180006183.01.T01:intron 35.0%
TCTGAACAAAAACTTGTGCT+TGG + Chr1:101076011-101076030 MsG0180006183.01.T01:CDS 35.0%
TTCATGTAATTACCGTAGGT+TGG + Chr1:101075848-101075867 MsG0180006183.01.T01:intron 35.0%
TTGTTGGATGAAATCAATGC+GGG - Chr1:101074440-101074459 None:intergenic 35.0%
TTTACCTTTGATGTCCTTTC+TGG + Chr1:101074712-101074731 MsG0180006183.01.T01:CDS 35.0%
TTTGAACTATTTCACTTGGG+TGG + Chr1:101074913-101074932 MsG0180006183.01.T01:intron 35.0%
TTTGTTCACCTGAGTAGTAT+TGG - Chr1:101075242-101075261 None:intergenic 35.0%
! AATCAATTCATGGACTGTGA+TGG - Chr1:101075469-101075488 None:intergenic 35.0%
! AGGTGTATTTTCAGTAACCA+AGG + Chr1:101075909-101075928 MsG0180006183.01.T01:CDS 35.0%
! TTTGAGTTTCCTTTGCTTGA+CGG - Chr1:101075354-101075373 None:intergenic 35.0%
! TTTTCAGTAACCAAGGTGTA+TGG + Chr1:101075916-101075935 MsG0180006183.01.T01:CDS 35.0%
! TTTTCGAAAGAGCCATGTTT+GGG - Chr1:101075598-101075617 None:intergenic 35.0%
!! ACTTCTTTGAAACATCTAGC+GGG - Chr1:101074693-101074712 None:intergenic 35.0%
!! GTTTTGTTGGAAACGTATGA+AGG - Chr1:101075735-101075754 None:intergenic 35.0%
!! TTTTTCGAAAGAGCCATGTT+TGG - Chr1:101075599-101075618 None:intergenic 35.0%
!!! TTTTGTTTCTGTTTAGGGGT+TGG + Chr1:101074631-101074650 MsG0180006183.01.T01:intron 35.0%
AAAAACTGGAGTCCTGTCAA+TGG + Chr1:101075615-101075634 MsG0180006183.01.T01:intron 40.0%
AAATATGTACCCTCCCAAAG+AGG - Chr1:101074511-101074530 None:intergenic 40.0%
AACCTGTCATAATGCCAGAA+AGG - Chr1:101074729-101074748 None:intergenic 40.0%
AATGCCAGAAAGGACATCAA+AGG - Chr1:101074719-101074738 None:intergenic 40.0%
AGTGCATAAGATGAAGAAGC+AGG - Chr1:101074797-101074816 None:intergenic 40.0%
CATGGCTCTTTCGAAAAAAC+TGG + Chr1:101075601-101075620 MsG0180006183.01.T01:intron 40.0%
GAACAAAAACTTGTGCTTGG+AGG + Chr1:101076014-101076033 MsG0180006183.01.T01:CDS 40.0%
GCATTATAGACAACATCCCA+AGG - Chr1:101075963-101075982 None:intergenic 40.0%
GCTTCATGTATTCCTTCTAG+TGG - Chr1:101075990-101076009 None:intergenic 40.0%
GTAACTTTAACCACCGGATA+TGG - Chr1:101075436-101075455 None:intergenic 40.0%
GTGCATAAGATGAAGAAGCA+GGG - Chr1:101074796-101074815 None:intergenic 40.0%
TATAATGCTGAGCCACTAGA+AGG + Chr1:101075975-101075994 MsG0180006183.01.T01:CDS 40.0%
TCGTTAAATCCGTCAAGCAA+AGG + Chr1:101075342-101075361 MsG0180006183.01.T01:intron 40.0%
TCTAACACTCACTCTCTTGT+TGG - Chr1:101074456-101074475 None:intergenic 40.0%
TGGGCCTTCATACAAAGTAT+TGG - Chr1:101075579-101075598 None:intergenic 40.0%
TGTATGGTATCTTGACCATG+TGG + Chr1:101075932-101075951 MsG0180006183.01.T01:CDS 40.0%
TGTTCTTAGCACTCCTCTTT+GGG + Chr1:101074495-101074514 MsG0180006183.01.T01:intron 40.0%
TTCCATTGATATGAAGTGGC+AGG - Chr1:101075106-101075125 None:intergenic 40.0%
TTCGTGCCAAATGAACTACA+TGG - Chr1:101075795-101075814 None:intergenic 40.0%
TTTGTATGAAGGCCCAAACA+TGG + Chr1:101075583-101075602 MsG0180006183.01.T01:intron 40.0%
! AAGCCTTTTGCAACAACCTT+CGG - Chr1:101075888-101075907 None:intergenic 40.0%
! TGGAAGACTCGACTTTTCTA+TGG - Chr1:101074555-101074574 None:intergenic 40.0%
! TTTTTCTTTGCCATATCCGG+TGG + Chr1:101075423-101075442 MsG0180006183.01.T01:intron 40.0%
!! AGCATCGATTTGACACAAAG+TGG - Chr1:101075180-101075199 None:intergenic 40.0%
AGGTTGTTGCAAAAGGCTTG+AGG + Chr1:101075889-101075908 MsG0180006183.01.T01:CDS 45.0%
CGCGATTATATCTCCAATGC+AGG + Chr1:101075518-101075537 MsG0180006183.01.T01:intron 45.0%
GATGAAGAAGCAGGGTAAGT+AGG - Chr1:101074788-101074807 None:intergenic 45.0%
GGGTTGGATATCCTGATCTT+TGG + Chr1:101074647-101074666 MsG0180006183.01.T01:CDS 45.0%
GGTCAAGATACCATACACCT+TGG - Chr1:101075929-101075948 None:intergenic 45.0%
GTGTTCTTAGCACTCCTCTT+TGG + Chr1:101074494-101074513 MsG0180006183.01.T01:intron 45.0%
TGACCATGTGGATGTACCTT+GGG + Chr1:101075944-101075963 MsG0180006183.01.T01:CDS 45.0%
TGGAGGAGAAGTTTGCATGT+GGG + Chr1:101076031-101076050 MsG0180006183.01.T01:CDS 45.0%
TTCCAACAAAACTCCCATCG+CGG + Chr1:101075743-101075762 MsG0180006183.01.T01:intron 45.0%
TTGACCATGTGGATGTACCT+TGG + Chr1:101075943-101075962 MsG0180006183.01.T01:CDS 45.0%
TTGCTGGACCAATACTACTC+AGG + Chr1:101075231-101075250 MsG0180006183.01.T01:CDS 45.0%
TTGGAGGAGAAGTTTGCATG+TGG + Chr1:101076030-101076049 MsG0180006183.01.T01:CDS 45.0%
! AGCCTTTTGCAACAACCTTC+GGG - Chr1:101075887-101075906 None:intergenic 45.0%
! GGTGGTGATGAAGTGAATAC+AGG + Chr1:101074931-101074950 MsG0180006183.01.T01:intron 45.0%
! GTCCTTTCTGGCATTATGAC+AGG + Chr1:101074724-101074743 MsG0180006183.01.T01:CDS 45.0%
! TGCCTGCCACTTCATATCAA+TGG + Chr1:101075101-101075120 MsG0180006183.01.T01:intron 45.0%
!! TGTGATTCTGAAGCCTGCAT+TGG - Chr1:101075534-101075553 None:intergenic 45.0%
AAACACCAGGTCCCAACCTA+CGG - Chr1:101075863-101075882 None:intergenic 50.0%
CATCCCAAGGTACATCCACA+TGG - Chr1:101075950-101075969 None:intergenic 50.0%
CGGGTCTTACCACTATACGT+GGG - Chr1:101074421-101074440 None:intergenic 50.0%
TCTTAGCACTCCTCTTTGGG+AGG + Chr1:101074498-101074517 MsG0180006183.01.T01:intron 50.0%
TGCCCGAAGGTTGTTGCAAA+AGG + Chr1:101075882-101075901 MsG0180006183.01.T01:CDS 50.0%
! AATTACCGTAGGTTGGGACC+TGG + Chr1:101075855-101075874 MsG0180006183.01.T01:intron 50.0%
! CTTAGCACTCCTCTTTGGGA+GGG + Chr1:101074499-101074518 MsG0180006183.01.T01:intron 50.0%
ACAACCTTCGGGCAAACACC+AGG - Chr1:101075876-101075895 None:intergenic 55.0%
GCGGGTCTTACCACTATACG+TGG - Chr1:101074422-101074441 None:intergenic 55.0%
TATGCACAACGGTCCGCGAT+GGG - Chr1:101075759-101075778 None:intergenic 55.0%
TCGCGGACCGTTGTGCATAA+TGG + Chr1:101075760-101075779 MsG0180006183.01.T01:intron 55.0%
TTATGCACAACGGTCCGCGA+TGG - Chr1:101075760-101075779 None:intergenic 55.0%
!! GTCCGCGATGGGAGTTTTGT+TGG - Chr1:101075748-101075767 None:intergenic 55.0%
GTGGGCGATGGCCAAAGATC+AGG - Chr1:101074661-101074680 None:intergenic 60.0%
TTTGGCCATCGCCCACGTGT+AGG + Chr1:101074665-101074684 MsG0180006183.01.T01:CDS 60.0%
!! ATCTAGCGGGCTCCTACACG+TGG - Chr1:101074680-101074699 None:intergenic 60.0%
!! TCTAGCGGGCTCCTACACGT+GGG - Chr1:101074679-101074698 None:intergenic 60.0%
GGGACCTGGTGTTTGCCCGA+AGG + Chr1:101075869-101075888 MsG0180006183.01.T01:CDS 65.0%
GGGCTCCTACACGTGGGCGA+TGG - Chr1:101074673-101074692 None:intergenic 70.0%
CCTCGCGCTGCAGCAGCAGC+AGG + Chr1:101076095-101076114 MsG0180006183.01.T01:CDS 75.0%
CCTGCTGCTGCTGCAGCGCG+AGG - Chr1:101076098-101076117 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr1 gene 101074412 101076121 101074412 ID=MsG0180006183.01;Name=MsG0180006183.01
Chr1 mRNA 101074412 101076121 101074412 ID=MsG0180006183.01.T01;Parent=MsG0180006183.01;Name=MsG0180006183.01.T01;_AED=0.46;_eAED=0.53;_QI=0|0|0.5|1|0.33|0.5|4|0|146
Chr1 exon 101074412 101074463 101074412 ID=MsG0180006183.01.T01:exon:12793;Parent=MsG0180006183.01.T01
Chr1 exon 101074647 101074745 101074647 ID=MsG0180006183.01.T01:exon:12794;Parent=MsG0180006183.01.T01
Chr1 exon 101075230 101075263 101075230 ID=MsG0180006183.01.T01:exon:12795;Parent=MsG0180006183.01.T01
Chr1 exon 101075866 101076121 101075866 ID=MsG0180006183.01.T01:exon:12796;Parent=MsG0180006183.01.T01
Chr1 CDS 101074412 101074463 101074412 ID=MsG0180006183.01.T01:cds;Parent=MsG0180006183.01.T01
Chr1 CDS 101074647 101074745 101074647 ID=MsG0180006183.01.T01:cds;Parent=MsG0180006183.01.T01
Chr1 CDS 101075230 101075263 101075230 ID=MsG0180006183.01.T01:cds;Parent=MsG0180006183.01.T01
Chr1 CDS 101075866 101076121 101075866 ID=MsG0180006183.01.T01:cds;Parent=MsG0180006183.01.T01
Gene Sequence

>MsG0180006183.01.T01

ATGGGTCCCACGTATAGTGGTAAGACCCGCATTGATTTCATCCAACAAGAGAGGGTTGGATATCCTGATCTTTGGCCATCGCCCACGTGTAGGAGCCCGCTAGATGTTTCAAAGAAGTTTACCTTTGATGTCCTTTCTGGCATTATGACAGATTGCTGGACCAATACTACTCAGGTGAACAAATGGTTGGGACCTGGTGTTTGCCCGAAGGTTGTTGCAAAAGGCTTGAGGTGTATTTTCAGTAACCAAGGTGTATGGTATCTTGACCATGTGGATGTACCTTGGGATGTTGTCTATAATGCTGAGCCACTAGAAGGAATACATGAAGCTTCTGAACAAAAACTTGTGCTTGGAGGAGAAGTTTGCATGTGGGCTGAGAGAGCTGATACATCAGATGTTCAGCAAACAATATGGCCTCGCGCTGCAGCAGCAGCAGGTTAG

Protein sequence

>MsG0180006183.01.T01

MGPTYSGKTRIDFIQQERVGYPDLWPSPTCRSPLDVSKKFTFDVLSGIMTDCWTNTTQVNKWLGPGVCPKVVAKGLRCIFSNQGVWYLDHVDVPWDVVYNAEPLEGIHEASEQKLVLGGEVCMWAERADTSDVQQTIWPRAAAAAG*