Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016412.01.T01 | XP_013461330.1 | 94.69 | 113 | 6 | 0 | 1 | 113 | 162 | 274 | 1.56E-65 | 209 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016412.01.T01 | A0A072VB49 | 94.690 | 113 | 6 | 0 | 1 | 113 | 162 | 274 | 7.44e-66 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002421.01 | MsG0380016412.01 | 0.837911 | 3.931092e-57 | 5.181163e-54 |
| MsG0180003427.01 | MsG0380016412.01 | 0.808316 | 3.288361e-50 | 1.874251e-47 |
| MsG0380016412.01 | MsG0380016703.01 | 0.802635 | 5.109797e-49 | 2.515593e-46 |
| MsG0380016412.01 | MsG0480020965.01 | 0.803245 | 3.822315e-49 | 1.911269e-46 |
| MsG0380016412.01 | MsG0480021013.01 | 0.804694 | 1.910000e-49 | 9.913477e-47 |
| MsG0380016412.01 | MsG0480022331.01 | 0.801770 | 7.699003e-49 | 3.707582e-46 |
| MsG0380016412.01 | MsG0580026186.01 | 0.800693 | 1.278454e-48 | 5.991348e-46 |
| MsG0380016412.01 | MsG0580029298.01 | 0.802255 | 6.117723e-49 | 2.982888e-46 |
| MsG0380016412.01 | MsG0780039861.01 | 0.819240 | 1.288224e-52 | 9.859032e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016412.01.T01 | MTR_3g088735 | 94.690 | 113 | 6 | 0 | 1 | 113 | 162 | 274 | 1.89e-69 | 209 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016412.01.T01 | AT1G21510 | 37.607 | 117 | 56 | 4 | 1 | 113 | 220 | 323 | 1.10e-12 | 63.2 |
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGTTAATTCGTCTTGTATA+AGG | 0.242757 | 3:+83090122 | MsG0380016412.01.T01:CDS |
| TTGCGGCGGGTTCAATCTTC+TGG | 0.269238 | 3:+83090187 | MsG0380016412.01.T01:CDS |
| TCATAATATTGACTCAATTA+TGG | 0.284715 | 3:+83090264 | MsG0380016412.01.T01:CDS |
| AATCTTCTGGTTTGAGTTTG+AGG | 0.310998 | 3:+83090200 | MsG0380016412.01.T01:CDS |
| ACCCGCCGCAAACTGTTGTT+TGG | 0.336354 | 3:-83090175 | None:intergenic |
| TGTTGTATGTACCACTATTT+CGG | 0.353636 | 3:+83090036 | MsG0380016412.01.T01:CDS |
| TGATAATCCTCTTATCTCAT+TGG | 0.365633 | 3:+83090294 | MsG0380016412.01.T01:CDS |
| GAATGAGCTACACCAGCCTT+AGG | 0.413781 | 3:-83090091 | None:intergenic |
| GCATAGAGAAACTGTGGTTA+AGG | 0.426925 | 3:+83090222 | MsG0380016412.01.T01:CDS |
| CTGAGGCCTAATAGAATGTT+GGG | 0.453271 | 3:+83089980 | None:intergenic |
| AACACTATTATCCGAAATAG+TGG | 0.462971 | 3:-83090047 | None:intergenic |
| AAACAGTCCAATGAGATAAG+AGG | 0.472907 | 3:-83090301 | None:intergenic |
| TCTCCGAGTCGTAGATTCAA+TGG | 0.488828 | 3:+83090010 | MsG0380016412.01.T01:CDS |
| AATGAGCTACACCAGCCTTA+GGG | 0.541293 | 3:-83090090 | None:intergenic |
| TCCAAACAACAGTTTGCGGC+GGG | 0.548089 | 3:+83090174 | MsG0380016412.01.T01:CDS |
| TTGAATCTACGACTCGGAGA+AGG | 0.589210 | 3:-83090007 | None:intergenic |
| GCGAATGAGTACTAGCCCTA+AGG | 0.600789 | 3:+83090075 | MsG0380016412.01.T01:CDS |
| ATGAGTACTAGCCCTAAGGC+TGG | 0.612037 | 3:+83090079 | MsG0380016412.01.T01:CDS |
| TTAGTCCAAACAACAGTTTG+CGG | 0.618157 | 3:+83090170 | MsG0380016412.01.T01:CDS |
| GTCCAAACAACAGTTTGCGG+CGG | 0.622915 | 3:+83090173 | MsG0380016412.01.T01:CDS |
| CTTCCATTGAATCTACGACT+CGG | 0.643674 | 3:-83090013 | None:intergenic |
| TTTGAGGCATAGAGAAACTG+TGG | 0.645417 | 3:+83090216 | MsG0380016412.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCATAATATTGACTCAATTA+TGG | + | Chr3:83090264-83090283 | MsG0380016412.01.T01:CDS | 20.0% |
| AACACTATTATCCGAAATAG+TGG | - | Chr3:83090050-83090069 | None:intergenic | 30.0% | |
| CTGTTAATTCGTCTTGTATA+AGG | + | Chr3:83090122-83090141 | MsG0380016412.01.T01:CDS | 30.0% | |
| TGATAATCCTCTTATCTCAT+TGG | + | Chr3:83090294-83090313 | MsG0380016412.01.T01:CDS | 30.0% | |
| TGTTGTATGTACCACTATTT+CGG | + | Chr3:83090036-83090055 | MsG0380016412.01.T01:CDS | 30.0% | |
| AAACAGTCCAATGAGATAAG+AGG | - | Chr3:83090304-83090323 | None:intergenic | 35.0% | |
| TTAGTCCAAACAACAGTTTG+CGG | + | Chr3:83090170-83090189 | MsG0380016412.01.T01:CDS | 35.0% | |
| !! | AATCTTCTGGTTTGAGTTTG+AGG | + | Chr3:83090200-83090219 | MsG0380016412.01.T01:CDS | 35.0% |
| CTTCCATTGAATCTACGACT+CGG | - | Chr3:83090016-83090035 | None:intergenic | 40.0% | |
| GCATAGAGAAACTGTGGTTA+AGG | + | Chr3:83090222-83090241 | MsG0380016412.01.T01:CDS | 40.0% | |
| ! | TTTGAGGCATAGAGAAACTG+TGG | + | Chr3:83090216-83090235 | MsG0380016412.01.T01:CDS | 40.0% |
| AATGAGCTACACCAGCCTTA+GGG | - | Chr3:83090093-83090112 | None:intergenic | 45.0% | |
| TCTCCGAGTCGTAGATTCAA+TGG | + | Chr3:83090010-83090029 | MsG0380016412.01.T01:CDS | 45.0% | |
| ! | TTGAATCTACGACTCGGAGA+AGG | - | Chr3:83090010-83090029 | None:intergenic | 45.0% |
| ATGAGTACTAGCCCTAAGGC+TGG | + | Chr3:83090079-83090098 | MsG0380016412.01.T01:CDS | 50.0% | |
| GAATGAGCTACACCAGCCTT+AGG | - | Chr3:83090094-83090113 | None:intergenic | 50.0% | |
| GCGAATGAGTACTAGCCCTA+AGG | + | Chr3:83090075-83090094 | MsG0380016412.01.T01:CDS | 50.0% | |
| GTCCAAACAACAGTTTGCGG+CGG | + | Chr3:83090173-83090192 | MsG0380016412.01.T01:CDS | 50.0% | |
| TCCAAACAACAGTTTGCGGC+GGG | + | Chr3:83090174-83090193 | MsG0380016412.01.T01:CDS | 50.0% | |
| ACCCGCCGCAAACTGTTGTT+TGG | - | Chr3:83090178-83090197 | None:intergenic | 55.0% | |
| ! | TTGCGGCGGGTTCAATCTTC+TGG | + | Chr3:83090187-83090206 | MsG0380016412.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 83089995 | 83090336 | 83089995 | ID=MsG0380016412.01;Name=MsG0380016412.01 |
| Chr3 | mRNA | 83089995 | 83090336 | 83089995 | ID=MsG0380016412.01.T01;Parent=MsG0380016412.01;Name=MsG0380016412.01.T01;_AED=0.37;_eAED=0.37;_QI=0|-1|0|1|-1|1|1|0|113 |
| Chr3 | exon | 83089995 | 83090336 | 83089995 | ID=MsG0380016412.01.T01:exon:13971;Parent=MsG0380016412.01.T01 |
| Chr3 | CDS | 83089995 | 83090336 | 83089995 | ID=MsG0380016412.01.T01:cds;Parent=MsG0380016412.01.T01 |
| Gene Sequence |
| Protein sequence |
