AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022331.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022331.01.T01 MTR_4g088385 84.573 363 48 6 1 360 1 358 1.05e-141 406
MsG0480022331.01.T01 MTR_2g014400 52.381 147 56 5 221 359 120 260 1.84e-17 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTGTTCTGGTTTGTTTAA+TGG 0.166365 4:-71270344 None:intergenic
GACTGTCTCTGGCATGAATT+TGG 0.206853 4:-71270247 None:intergenic
TTCGAGTTATATCCTTCTTC+TGG 0.209481 4:-71270683 None:intergenic
ATTCGGGTACTTGTTGTTAT+AGG 0.218132 4:-71270555 None:intergenic
CTTCAAACTGCTCTTGATTC+TGG 0.250818 4:-71271222 None:intergenic
TTTAATGGTGTTTCATTGTT+AGG 0.261763 4:-71270329 None:intergenic
TGTTGTTGTTGTTATTGTTC+TGG 0.280406 4:-71270820 None:intergenic
ATGAAAACTGGTTGTTGTTC+TGG 0.303072 4:-71270356 None:intergenic
CAAGTATTCTTCTAGTTCTA+AGG 0.327940 4:+71270640 MsG0480022331.01.T01:CDS
GAAAACAGTTATGGTTTATA+TGG 0.334786 4:+71270389 MsG0480022331.01.T01:CDS
GGTTTATATGGTCATGATAC+TGG 0.336006 4:+71270401 MsG0480022331.01.T01:CDS
ATTGAGTTGTAACCTTCTTC+TGG 0.340437 4:-71270887 None:intergenic
TTCATATGATTGGAAAGTGT+TGG 0.393181 4:-71270465 None:intergenic
TCGGTTTCCGGGATGAAAAC+TGG 0.395977 4:-71270368 None:intergenic
GCTGAAGAATTGACTGTCTC+TGG 0.398942 4:-71270258 None:intergenic
TTGGTGTCACTAACTTCATT+CGG 0.404949 4:-71270572 None:intergenic
TGTTGTTATAGGAATCTTGC+TGG 0.405186 4:-71270544 None:intergenic
TCATATGATTGGAAAGTGTT+GGG 0.422874 4:-71270464 None:intergenic
GTTGTCGTAGTTGTTGTTGT+TGG 0.424284 4:-71270615 None:intergenic
CCGGAAACCGAAAACAGTTA+TGG 0.426235 4:+71270380 MsG0480022331.01.T01:CDS
TCGAGTTATATCCTTCTTCT+GGG 0.432731 4:-71270682 None:intergenic
TGGTGTCACTAACTTCATTC+GGG 0.435111 4:-71270571 None:intergenic
TTGAGTTGTAACCTTCTTCT+GGG 0.440468 4:-71270886 None:intergenic
GTAAGATGTTCCCGTTAGTT+TGG 0.449964 4:-71270591 None:intergenic
AACGCTAAGTTTACTGAAGA+AGG 0.456600 4:+71270779 MsG0480022331.01.T01:CDS
TTCAATGGAGATTCATCTAA+AGG 0.457617 4:+71271103 MsG0480022331.01.T01:CDS
AATGAGTGACACAAGAGTTA+TGG 0.465302 4:+71271027 MsG0480022331.01.T01:CDS
ATGAAAGGGTTGTTAAGAAT+AGG 0.475071 4:-71270217 None:intergenic
AAATACAATCCAACATTCAA+TGG 0.477159 4:+71271088 MsG0480022331.01.T01:CDS
TTGTTTGTGTTAGTAGTACT+AGG 0.484396 4:-71270434 None:intergenic
GTTAGTGACACCAAACTAAC+GGG 0.485500 4:+71270581 MsG0480022331.01.T01:CDS
GAGTTGTGAACTCTGAGAAT+TGG 0.491359 4:+71271125 MsG0480022331.01.T01:CDS
GATGAATCTCCATTGAATGT+TGG 0.492045 4:-71271097 None:intergenic
TTGTTAAGAATAGGAAAGAA+AGG 0.493863 4:-71270208 None:intergenic
TTGTGTTAGTAGTACTAGGA+GGG 0.504780 4:-71270430 None:intergenic
AACACAAAGTTCCCAGAAGA+AGG 0.504928 4:+71270875 MsG0480022331.01.T01:CDS
GAATTTGGAGAGAAAATGAA+AGG 0.529100 4:-71270232 None:intergenic
AATAACAAGAACTCTATGAA+TGG 0.533344 4:+71271196 MsG0480022331.01.T01:CDS
TACAACAATGGTGAGAGGCA+AGG 0.586180 4:+71271004 MsG0480022331.01.T01:CDS
TGAAGAAGGATACAAATCCA+TGG 0.589242 4:+71270793 MsG0480022331.01.T01:CDS
TTTGTGTTAGTAGTACTAGG+AGG 0.589979 4:-71270431 None:intergenic
AATTTGGAGAGAAAATGAAA+GGG 0.593601 4:-71270231 None:intergenic
AGTTAGTGACACCAAACTAA+CGG 0.603223 4:+71270580 MsG0480022331.01.T01:CDS
AGTAACAACAATTACAACAA+TGG 0.621284 4:+71270992 MsG0480022331.01.T01:CDS
GACACAAGAGTTATGGAAGG+TGG 0.628338 4:+71271034 MsG0480022331.01.T01:CDS
ACAATTACAACAATGGTGAG+AGG 0.652438 4:+71270999 MsG0480022331.01.T01:CDS
AGTGACACAAGAGTTATGGA+AGG 0.692016 4:+71271031 MsG0480022331.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATAAACCATAACTGTTTT+CGG - Chr4:71270390-71270409 None:intergenic 20.0%
! AAATACAATCCAACATTCAA+TGG + Chr4:71271088-71271107 MsG0480022331.01.T01:CDS 25.0%
! AATAACAAGAACTCTATGAA+TGG + Chr4:71271196-71271215 MsG0480022331.01.T01:CDS 25.0%
! AATTTGGAGAGAAAATGAAA+GGG - Chr4:71270234-71270253 None:intergenic 25.0%
! AGTAACAACAATTACAACAA+TGG + Chr4:71270992-71271011 MsG0480022331.01.T01:CDS 25.0%
! GAAAACAGTTATGGTTTATA+TGG + Chr4:71270389-71270408 MsG0480022331.01.T01:CDS 25.0%
! TACAACAAAAAAGGTTACTT+TGG + Chr4:71271148-71271167 MsG0480022331.01.T01:CDS 25.0%
! TTGTTAAGAATAGGAAAGAA+AGG - Chr4:71270211-71270230 None:intergenic 25.0%
! TTTAATGGTGTTTCATTGTT+AGG - Chr4:71270332-71270351 None:intergenic 25.0%
!! TTTTTATGATGTTAACTCTG+AGG + Chr4:71271063-71271082 MsG0480022331.01.T01:CDS 25.0%
!!! AAAGAAAGGAATTTTGAGAT+GGG - Chr4:71270197-71270216 None:intergenic 25.0%
!!! ACGAAGTTTTTTCATATGAT+TGG - Chr4:71270478-71270497 None:intergenic 25.0%
ATGAAAGGGTTGTTAAGAAT+AGG - Chr4:71270220-71270239 None:intergenic 30.0%
GAATTTGGAGAGAAAATGAA+AGG - Chr4:71270235-71270254 None:intergenic 30.0%
GAGAATTGGTACAACAAAAA+AGG + Chr4:71271139-71271158 MsG0480022331.01.T01:CDS 30.0%
TTCAATGGAGATTCATCTAA+AGG + Chr4:71271103-71271122 MsG0480022331.01.T01:CDS 30.0%
TTGTTTGTGTTAGTAGTACT+AGG - Chr4:71270437-71270456 None:intergenic 30.0%
TTTCTACAACAACAACAACA+AGG + Chr4:71270745-71270764 MsG0480022331.01.T01:CDS 30.0%
! TGTTGTTGTTGTTATTGTTC+TGG - Chr4:71270823-71270842 None:intergenic 30.0%
! TTCATATGATTGGAAAGTGT+TGG - Chr4:71270468-71270487 None:intergenic 30.0%
!! CAAGTATTCTTCTAGTTCTA+AGG + Chr4:71270640-71270659 MsG0480022331.01.T01:CDS 30.0%
!! GAAAGAAAGGAATTTTGAGA+TGG - Chr4:71270198-71270217 None:intergenic 30.0%
!! TCATATGATTGGAAAGTGTT+GGG - Chr4:71270467-71270486 None:intergenic 30.0%
!! TGTTGTTCTGGTTTGTTTAA+TGG - Chr4:71270347-71270366 None:intergenic 30.0%
AACAAAAAGTTCCCAGAAGA+AGG + Chr4:71270671-71270690 MsG0480022331.01.T01:CDS 35.0%
AACGCTAAGTTTACTGAAGA+AGG + Chr4:71270779-71270798 MsG0480022331.01.T01:CDS 35.0%
AATGAGTGACACAAGAGTTA+TGG + Chr4:71271027-71271046 MsG0480022331.01.T01:CDS 35.0%
ACAATTACAACAATGGTGAG+AGG + Chr4:71270999-71271018 MsG0480022331.01.T01:CDS 35.0%
AGTTAGTGACACCAAACTAA+CGG + Chr4:71270580-71270599 MsG0480022331.01.T01:CDS 35.0%
GATGAATCTCCATTGAATGT+TGG - Chr4:71271100-71271119 None:intergenic 35.0%
GGTTTATATGGTCATGATAC+TGG + Chr4:71270401-71270420 MsG0480022331.01.T01:CDS 35.0%
TCGAGTTATATCCTTCTTCT+GGG - Chr4:71270685-71270704 None:intergenic 35.0%
TGAAGAAGGATACAAATCCA+TGG + Chr4:71270793-71270812 MsG0480022331.01.T01:CDS 35.0%
TGTTGTTATAGGAATCTTGC+TGG - Chr4:71270547-71270566 None:intergenic 35.0%
TTGTGTTAGTAGTACTAGGA+GGG - Chr4:71270433-71270452 None:intergenic 35.0%
TTTGTGTTAGTAGTACTAGG+AGG - Chr4:71270434-71270453 None:intergenic 35.0%
! ATGAAAACTGGTTGTTGTTC+TGG - Chr4:71270359-71270378 None:intergenic 35.0%
! ATTGAGTTGTAACCTTCTTC+TGG - Chr4:71270890-71270909 None:intergenic 35.0%
! GTTGTTGTTGTTGTTGACAT+GGG - Chr4:71270291-71270310 None:intergenic 35.0%
! TGTTGTTGTTGTTGTTGACA+TGG - Chr4:71270292-71270311 None:intergenic 35.0%
! TTCGAGTTATATCCTTCTTC+TGG - Chr4:71270686-71270705 None:intergenic 35.0%
! TTGAGTTGTAACCTTCTTCT+GGG - Chr4:71270889-71270908 None:intergenic 35.0%
!! ATTCGGGTACTTGTTGTTAT+AGG - Chr4:71270558-71270577 None:intergenic 35.0%
!! TTGGTGTCACTAACTTCATT+CGG - Chr4:71270575-71270594 None:intergenic 35.0%
!!! GTTATTGTTCTGGTTTTCCA+TGG - Chr4:71270813-71270832 None:intergenic 35.0%
AACACAAAGTTCCCAGAAGA+AGG + Chr4:71270875-71270894 MsG0480022331.01.T01:CDS 40.0%
AGTGACACAAGAGTTATGGA+AGG + Chr4:71271031-71271050 MsG0480022331.01.T01:CDS 40.0%
CTTCAAACTGCTCTTGATTC+TGG - Chr4:71271225-71271244 None:intergenic 40.0%
GTAAGATGTTCCCGTTAGTT+TGG - Chr4:71270594-71270613 None:intergenic 40.0%
GTTAGTGACACCAAACTAAC+GGG + Chr4:71270581-71270600 MsG0480022331.01.T01:CDS 40.0%
! ACAACAACCAGTTTTCATCC+CGG + Chr4:71270361-71270380 MsG0480022331.01.T01:CDS 40.0%
! CATAACTGTTTTCGGTTTCC+GGG - Chr4:71270382-71270401 None:intergenic 40.0%
! CCATAACTGTTTTCGGTTTC+CGG - Chr4:71270383-71270402 None:intergenic 40.0%
! CGTAGTTGTTGTTGTTGTTC+TGG - Chr4:71270919-71270938 None:intergenic 40.0%
! GAGTTGTGAACTCTGAGAAT+TGG + Chr4:71271125-71271144 MsG0480022331.01.T01:CDS 40.0%
! GTTGTCGTAGTTGTTGTTGT+TGG - Chr4:71270618-71270637 None:intergenic 40.0%
!! TGGTGTCACTAACTTCATTC+GGG - Chr4:71270574-71270593 None:intergenic 40.0%
CCGGAAACCGAAAACAGTTA+TGG + Chr4:71270380-71270399 MsG0480022331.01.T01:CDS 45.0%
GACACAAGAGTTATGGAAGG+TGG + Chr4:71271034-71271053 MsG0480022331.01.T01:CDS 45.0%
GACTGTCTCTGGCATGAATT+TGG - Chr4:71270250-71270269 None:intergenic 45.0%
GCTGAAGAATTGACTGTCTC+TGG - Chr4:71270261-71270280 None:intergenic 45.0%
TACAACAATGGTGAGAGGCA+AGG + Chr4:71271004-71271023 MsG0480022331.01.T01:CDS 45.0%
TCGGTTTCCGGGATGAAAAC+TGG - Chr4:71270371-71270390 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr4 gene 71270188 71271270 71270188 ID=MsG0480022331.01;Name=MsG0480022331.01
Chr4 mRNA 71270188 71271270 71270188 ID=MsG0480022331.01.T01;Parent=MsG0480022331.01;Name=MsG0480022331.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|360
Chr4 exon 71270188 71271270 71270188 ID=MsG0480022331.01.T01:exon:4837;Parent=MsG0480022331.01.T01
Chr4 CDS 71270188 71271270 71270188 ID=MsG0480022331.01.T01:cds;Parent=MsG0480022331.01.T01
Gene Sequence

>MsG0480022331.01.T01

ATGGCTCCCATCTCAAAATTCCTTTCTTTCCTATTCTTAACAACCCTTTCATTTTCTCTCCAAATTCATGCCAGAGACAGTCAATTCTTCAGCAAAGTCACCCATGTCAACAACAACAACAACAACAATGTTAGAGAAATTCCTAACAATGAAACACCATTAAACAAACCAGAACAACAACCAGTTTTCATCCCGGAAACCGAAAACAGTTATGGTTTATATGGTCATGATACTGGTCTACACCCTCCTAGTACTACTAACACAAACAATGTTGATCCCAACACTTTCCAATCATATGAAAAAACTTCGTCACACGATGAAATCAACGACAATAAAGTTAACAATTACTTCAACTACCAGCAAGATTCCTATAACAACAAGTACCCGAATGAAGTTAGTGACACCAAACTAACGGGAACATCTTACACCAACAACAACAACTACGACAACAACAAGTATTCTTCTAGTTCTAAGGACTTTAGCAACAAAAAGTTCCCAGAAGAAGGATATAACTCGAATGAAAATCAGAACAACAACAACAACAACGATAACAAGTATTTCTACAACAACAACAACAAGGATTCTTCTAGCAACGCTAAGTTTACTGAAGAAGGATACAAATCCATGGAAAACCAGAACAATAACAACAACAACAAGTATTTCTACAACAACAAAGATGCTTCTAGCAACACAAAGTTCCCAGAAGAAGGTTACAACTCAATGCAAAACCAGAACAACAACAACAACTACGAGAAGTATAACTACAACAACAAAGTTGCTGTTAATGACAAATACAGCTTCAAAAGTAACAACAATTACAACAATGGTGAGAGGCAAGGAATGAGTGACACAAGAGTTATGGAAGGTGGAAAATATTTTTATGATGTTAACTCTGAGGAGAAATACAATCCAACATTCAATGGAGATTCATCTAAAGGAGTTGTGAACTCTGAGAATTGGTACAACAAAAAAGGTTACTTTGGTAACAACAATGTGAACAATTATGAGAATAACAAGAACTCTATGAATGGTTACCAGAATCAAGAGCAGTTTGAAGATGATCAAGAAGAATTTGAGCCTTGA

Protein sequence

>MsG0480022331.01.T01

MAPISKFLSFLFLTTLSFSLQIHARDSQFFSKVTHVNNNNNNNVREIPNNETPLNKPEQQPVFIPETENSYGLYGHDTGLHPPSTTNTNNVDPNTFQSYEKTSSHDEINDNKVNNYFNYQQDSYNNKYPNEVSDTKLTGTSYTNNNNYDNNKYSSSSKDFSNKKFPEEGYNSNENQNNNNNNDNKYFYNNNNKDSSSNAKFTEEGYKSMENQNNNNNNKYFYNNKDASSNTKFPEEGYNSMQNQNNNNNYEKYNYNNKVAVNDKYSFKSNNNYNNGERQGMSDTRVMEGGKYFYDVNSEEKYNPTFNGDSSKGVVNSENWYNKKGYFGNNNVNNYENNKNSMNGYQNQEQFEDDQEEFEP*