Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016546.01.T01 | XP_013461431.1 | 55.769 | 104 | 18 | 3 | 1 | 103 | 1 | 77 | 9.81E-24 | 97.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016546.01.T01 | A0A072V1A4 | 55.769 | 104 | 18 | 3 | 1 | 103 | 1 | 77 | 4.69e-24 | 97.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000482.01 | MsG0380016546.01 | 0.808990 | 2.360043e-50 | 1.369132e-47 |
| MsG0180005220.01 | MsG0380016546.01 | 0.840515 | 8.300100e-58 | 1.185166e-54 |
| MsG0180005240.01 | MsG0380016546.01 | 0.839284 | 1.736850e-57 | 2.387188e-54 |
| MsG0280010599.01 | MsG0380016546.01 | 0.811637 | 6.337926e-51 | 3.943541e-48 |
| MsG0380016546.01 | MsG0680031815.01 | 0.813549 | 2.420170e-51 | 1.585284e-48 |
| MsG0380016546.01 | MsG0780038907.01 | 0.825428 | 4.712851e-54 | 4.294243e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380016546.01.T01 | MTR_3g491850 | 55.769 | 104 | 18 | 3 | 1 | 103 | 1 | 77 | 1.19e-27 | 97.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 39 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCCAGGTTTCTTTGAATGCT+TGG | 0.243739 | 3:+84993246 | None:intergenic |
| CCAGGTTTCTTTGAATGCTT+GGG | 0.302783 | 3:+84993247 | None:intergenic |
| ATTTGTTTACCTGAAGATAA+AGG | 0.340472 | 3:+84993799 | None:intergenic |
| AAACCAAGAGGATGGCTAAA+AGG | 0.364847 | 3:+84993833 | None:intergenic |
| TTTCTTTGAATGCTTGGGTT+GGG | 0.390693 | 3:+84993252 | None:intergenic |
| TTGTAAAGAAAGTTATTAGT+AGG | 0.421064 | 3:+84993666 | None:intergenic |
| CAAATCTTACTTGATATAAG+TGG | 0.429669 | 3:-84993781 | MsG0380016546.01.T01:intron |
| GTTTCTTTGAATGCTTGGGT+TGG | 0.434858 | 3:+84993251 | None:intergenic |
| TACTAATAACTTTCTTTACA+AGG | 0.464774 | 3:-84993664 | MsG0380016546.01.T01:CDS |
| TATGATTAGAATTAGTGTTA+AGG | 0.465816 | 3:+84993205 | None:intergenic |
| GTTTAATAACGACCCTTGCC+AGG | 0.478973 | 3:+84993229 | None:intergenic |
| AGTACTTTGGTATTGAATGA+AGG | 0.483995 | 3:-84993342 | MsG0380016546.01.T01:CDS |
| CCCAAGCATTCAAAGAAACC+TGG | 0.538447 | 3:-84993247 | MsG0380016546.01.T01:CDS |
| ATGAATGAAAACCAAGAGGA+TGG | 0.580545 | 3:+84993825 | None:intergenic |
| GTGTTGGATTATGATGAAGC+AGG | 0.583814 | 3:-84993276 | MsG0380016546.01.T01:CDS |
| GCATTCAAAGAAACCTGGCA+AGG | 0.600825 | 3:-84993242 | MsG0380016546.01.T01:CDS |
| CTTTGGTATTGAATGAAGGA+TGG | 0.604697 | 3:-84993338 | MsG0380016546.01.T01:CDS |
| CATTCAAAGAAACCTGGCAA+GGG | 0.630511 | 3:-84993241 | MsG0380016546.01.T01:CDS |
| GAAAATGAATGAAAACCAAG+AGG | 0.671422 | 3:+84993821 | None:intergenic |
| ATGGTGTAAAGATGAACTCG+AGG | 0.691234 | 3:-84993319 | MsG0380016546.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATTACAATTTTTTTTAT+AGG | + | Chr3:84993624-84993643 | None:intergenic | 10.0% |
| !!! | TTATTTGTAAACATTATTTT+GGG | + | Chr3:84993589-84993608 | None:intergenic | 10.0% |
| !!! | TTTATTTGTAAACATTATTT+TGG | + | Chr3:84993590-84993609 | None:intergenic | 10.0% |
| !!! | TTTTTTCATATAGTTTTAAA+TGG | - | Chr3:84993415-84993434 | MsG0380016546.01.T01:intron | 10.0% |
| !!! | AAAAAATTGTAATCTTTTTG+TGG | - | Chr3:84993628-84993647 | MsG0380016546.01.T01:intron | 15.0% |
| !!! | AATTTATTTTTTGCTCTTTT+AGG | - | Chr3:84993324-84993343 | MsG0380016546.01.T01:CDS | 15.0% |
| !! | AAAATTAAGATGAATACAGA+TGG | - | Chr3:84993498-84993517 | MsG0380016546.01.T01:intron | 20.0% |
| !! | CTATATGAAAAAAGATAGTA+AGG | + | Chr3:84993408-84993427 | None:intergenic | 20.0% |
| !! | TACTAATAACTTTCTTTACA+AGG | - | Chr3:84993380-84993399 | MsG0380016546.01.T01:intron | 20.0% |
| !! | TATGATTAGAATTAGTGTTA+AGG | + | Chr3:84993842-84993861 | None:intergenic | 20.0% |
| !! | TTGTAAAGAAAGTTATTAGT+AGG | + | Chr3:84993381-84993400 | None:intergenic | 20.0% |
| !!! | AACATTATTTTGGGATTATA+TGG | + | Chr3:84993580-84993599 | None:intergenic | 20.0% |
| !!! | ACATTATTTTGGGATTATAT+GGG | + | Chr3:84993579-84993598 | None:intergenic | 20.0% |
| !!! | TTTTTAACTAACAAGTACTT+TGG | - | Chr3:84993689-84993708 | MsG0380016546.01.T01:CDS | 20.0% |
| ! | ATTTGTTTACCTGAAGATAA+AGG | + | Chr3:84993248-84993267 | None:intergenic | 25.0% |
| ! | CAAATCTTACTTGATATAAG+TGG | - | Chr3:84993263-84993282 | MsG0380016546.01.T01:CDS | 25.0% |
| !! | ATTCATTTTCCTTTATCTTC+AGG | - | Chr3:84993236-84993255 | MsG0380016546.01.T01:CDS | 25.0% |
| !! | TATGTTCATCTTAAGTATTG+TGG | + | Chr3:84993467-84993486 | None:intergenic | 25.0% |
| !!! | ATTTTTTGCTCTTTTAGGAT+TGG | - | Chr3:84993329-84993348 | MsG0380016546.01.T01:CDS | 25.0% |
| GAAAATGAATGAAAACCAAG+AGG | + | Chr3:84993226-84993245 | None:intergenic | 30.0% | |
| ! | AGTACTTTGGTATTGAATGA+AGG | - | Chr3:84993702-84993721 | MsG0380016546.01.T01:intron | 30.0% |
| ATGAATGAAAACCAAGAGGA+TGG | + | Chr3:84993222-84993241 | None:intergenic | 35.0% | |
| GGCAAATGCTAATATTTGCT+TGG | - | Chr3:84993541-84993560 | MsG0380016546.01.T01:intron | 35.0% | |
| TTTCTTTGAATGCTTGGGTT+GGG | + | Chr3:84993795-84993814 | None:intergenic | 35.0% | |
| ! | ATTGTCTCATGCTTTTGTGT+TGG | - | Chr3:84993752-84993771 | MsG0380016546.01.T01:intron | 35.0% |
| ! | CTTTGGTATTGAATGAAGGA+TGG | - | Chr3:84993706-84993725 | MsG0380016546.01.T01:intron | 35.0% |
| AAACCAAGAGGATGGCTAAA+AGG | + | Chr3:84993214-84993233 | None:intergenic | 40.0% | |
| ATGGTGTAAAGATGAACTCG+AGG | - | Chr3:84993725-84993744 | MsG0380016546.01.T01:intron | 40.0% | |
| CATTCAAAGAAACCTGGCAA+GGG | - | Chr3:84993803-84993822 | MsG0380016546.01.T01:CDS | 40.0% | |
| CCAGGTTTCTTTGAATGCTT+GGG | + | Chr3:84993800-84993819 | None:intergenic | 40.0% | |
| GAGCTACTACATTCACATTG+AGG | - | Chr3:84993520-84993539 | MsG0380016546.01.T01:intron | 40.0% | |
| GTGTTGGATTATGATGAAGC+AGG | - | Chr3:84993768-84993787 | MsG0380016546.01.T01:intron | 40.0% | |
| GTTTCTTTGAATGCTTGGGT+TGG | + | Chr3:84993796-84993815 | None:intergenic | 40.0% | |
| ! | TTTCCTTTTAGCCATCCTCT+TGG | - | Chr3:84993208-84993227 | MsG0380016546.01.T01:CDS | 40.0% |
| CCCAAGCATTCAAAGAAACC+TGG | - | Chr3:84993797-84993816 | MsG0380016546.01.T01:CDS | 45.0% | |
| GCATTCAAAGAAACCTGGCA+AGG | - | Chr3:84993802-84993821 | MsG0380016546.01.T01:CDS | 45.0% | |
| GCCAGGTTTCTTTGAATGCT+TGG | + | Chr3:84993801-84993820 | None:intergenic | 45.0% | |
| GTTTAATAACGACCCTTGCC+AGG | + | Chr3:84993818-84993837 | None:intergenic | 45.0% | |
| !!! | GCTCTTTTAGGATTGGCTGA+AGG | - | Chr3:84993336-84993355 | MsG0380016546.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 84993188 | 84993878 | 84993188 | ID=MsG0380016546.01;Name=MsG0380016546.01 |
| Chr3 | mRNA | 84993188 | 84993878 | 84993188 | ID=MsG0380016546.01.T01;Parent=MsG0380016546.01;Name=MsG0380016546.01.T01;_AED=0.28;_eAED=0.50;_QI=0|0|0|1|0|0|3|0|120 |
| Chr3 | exon | 84993782 | 84993878 | 84993782 | ID=MsG0380016546.01.T01:exon:14419;Parent=MsG0380016546.01.T01 |
| Chr3 | exon | 84993630 | 84993720 | 84993630 | ID=MsG0380016546.01.T01:exon:14418;Parent=MsG0380016546.01.T01 |
| Chr3 | exon | 84993188 | 84993362 | 84993188 | ID=MsG0380016546.01.T01:exon:14417;Parent=MsG0380016546.01.T01 |
| Chr3 | CDS | 84993782 | 84993878 | 84993782 | ID=MsG0380016546.01.T01:cds;Parent=MsG0380016546.01.T01 |
| Chr3 | CDS | 84993630 | 84993720 | 84993630 | ID=MsG0380016546.01.T01:cds;Parent=MsG0380016546.01.T01 |
| Chr3 | CDS | 84993188 | 84993362 | 84993188 | ID=MsG0380016546.01.T01:cds;Parent=MsG0380016546.01.T01 |
| Gene Sequence |
| Protein sequence |
