AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780038907.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038907.01.T01 MTR_7g065080 92.063 63 1 1 1 59 434 496 3.97e-32 115
MsG0780038907.01.T01 MTR_7g065080 92.063 63 1 1 1 59 521 583 9.90e-32 114
MsG0780038907.01.T01 MTR_4g019580 69.643 56 16 1 1 56 532 586 4.47e-19 79.0
MsG0780038907.01.T01 MTR_3g069500 54.545 55 25 0 2 56 524 578 1.46e-16 71.6
MsG0780038907.01.T01 MTR_3g069440 52.632 57 25 1 1 57 531 585 5.64e-14 64.3
MsG0780038907.01.T01 MTR_3g069420 58.537 41 17 0 2 42 533 573 4.70e-13 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780038907.01.T01 AT3G53960 65.306 49 16 1 1 48 536 584 5.62e-15 67.4
MsG0780038907.01.T01 AT2G37900 60.976 41 16 0 1 41 450 490 3.14e-12 59.3
MsG0780038907.01.T01 AT2G37900 60.976 41 16 0 1 41 529 569 3.76e-12 59.3

Find 6 sgRNAs with CRISPR-Local

Find 8 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTGTGTTCTTTGCTAGA+AGG 0.310521 7:-55212680 MsG0780038907.01.T01:CDS
GGATTTGAATAGTAGTAGAT+TGG 0.408762 7:-55212751 MsG0780038907.01.T01:CDS
TGGGAAGAGTTGGTTTGGTA+AGG 0.432135 7:-55212772 None:intergenic
AGGCAAAAGTGTAGACAATT+CGG 0.457366 7:-55212607 MsG0780038907.01.T01:CDS
GCTATTGGCAGCTATAACTA+CGG 0.504079 7:-55212715 MsG0780038907.01.T01:CDS
GTTGTTGATGATAGTTGTAA+AGG 0.549754 7:-55212627 MsG0780038907.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TTCATACAAAAATGTGCAAA+AGG - Chr7:55212706-55212725 MsG0780038907.01.T01:CDS 25.0%
!! GTAGATTGGACAAATTTTAT+TGG - Chr7:55212624-55212643 MsG0780038907.01.T01:CDS 25.0%
GGATTTGAATAGTAGTAGAT+TGG - Chr7:55212610-55212629 MsG0780038907.01.T01:CDS 30.0%
!! GGACAAATTTTATTGGCTAT+TGG - Chr7:55212631-55212650 MsG0780038907.01.T01:CDS 30.0%
!! GTTGTTGATGATAGTTGTAA+AGG - Chr7:55212734-55212753 MsG0780038907.01.T01:CDS 30.0%
TGTTTGTGTTCTTTGCTAGA+AGG - Chr7:55212681-55212700 MsG0780038907.01.T01:CDS 35.0%
! AGGCAAAAGTGTAGACAATT+CGG - Chr7:55212754-55212773 MsG0780038907.01.T01:CDS 35.0%
GCTATTGGCAGCTATAACTA+CGG - Chr7:55212646-55212665 MsG0780038907.01.T01:CDS 40.0%
Chromosome Type Strat End Strand Name
Chr7 gene 55212602 55212781 55212602 ID=MsG0780038907.01;Name=MsG0780038907.01
Chr7 mRNA 55212602 55212781 55212602 ID=MsG0780038907.01.T01;Parent=MsG0780038907.01;Name=MsG0780038907.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|59
Chr7 exon 55212602 55212781 55212602 ID=MsG0780038907.01.T01:exon:23940;Parent=MsG0780038907.01.T01
Chr7 CDS 55212602 55212781 55212602 ID=MsG0780038907.01.T01:cds;Parent=MsG0780038907.01.T01
Gene Sequence

>MsG0780038907.01.T01

TTTGGTAAGGATTTGAATAGTAGTAGATTGGACAAATTTTATTGGCTATTGGCAGCTATAACTACGGTGAATTTGTTCTTGTTTGTGTTCTTTGCTAGAAGGTATTCATACAAAAATGTGCAAAAGGTTGCAGTTGTTGATGATAGTTGTAAAGGCAAAAGTGTAGACAATTCGGTTTGA

Protein sequence

>MsG0780038907.01.T01

FGKDLNSSRLDKFYWLLAAITTVNLFLFVFFARRYSYKNVQKVAVVDDSCKGKSVDNSV*