AlfalfaGEDB | MsG0180000482.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000482.01

KEGG: -
GO: -
PFAM: PF07911

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000482.01.T01	XP_013465872.1	98.658	149	2	0	1	149	1	149	7.10E-104	303

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000482.01.T01	A0A072VER7	98.658	149	2	0	1	149	1	149	3.39e-104	303

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048016.01	MsG0180000482.01	0.840322	9.324895e-58	1.323385e-54
MsG0080048614.01	MsG0180000482.01	0.801547	8.554231e-49	4.096314e-46
MsG0080048802.01	MsG0180000482.01	0.815897	7.304356e-52	5.099472e-49
MsG0080048938.01	MsG0180000482.01	0.842947	1.894471e-58	2.918122e-55
MsG0080049011.01	MsG0180000482.01	0.814908	1.212304e-51	8.237781e-49
MsG0080049040.01	MsG0180000482.01	0.815108	1.094281e-51	7.476364e-49
MsG0080049068.01	MsG0180000482.01	0.815870	7.408966e-52	5.168683e-49
MsG0080049069.01	MsG0180000482.01	0.808662	2.773921e-50	1.595307e-47
MsG0180000114.01	MsG0180000482.01	0.848616	5.470819e-60	1.009734e-56
MsG0180000246.01	MsG0180000482.01	0.834465	2.950251e-56	3.504190e-53
MsG0180000481.01	MsG0180000482.01	0.831919	1.270239e-55	1.398388e-52
MsG0180000482.01	MsG0180000599.01	0.849584	2.944620e-60	5.608399e-57
MsG0180000482.01	MsG0180000784.01	0.805650	1.204942e-49	6.409649e-47
MsG0180000482.01	MsG0180000785.01	0.812057	5.133513e-51	3.230402e-48
MsG0180000482.01	MsG0180000814.01	0.804312	2.294749e-49	1.179354e-46
MsG0180000482.01	MsG0180001100.01	0.860576	1.875690e-63	5.151458e-60
MsG0180000482.01	MsG0180001105.01	0.817669	2.925591e-52	2.144130e-49
MsG0180000482.01	MsG0180001594.01	0.804924	1.710343e-49	8.930598e-47
MsG0180000482.01	MsG0180002399.01	0.826758	2.275930e-54	2.153907e-51
MsG0180000482.01	MsG0180002700.01	0.804362	2.240700e-49	1.153062e-46
MsG0180000482.01	MsG0180003189.01	0.804897	1.732573e-49	9.040648e-47
MsG0180000482.01	MsG0180003353.01	0.802996	4.303472e-49	2.138096e-46
MsG0180000482.01	MsG0180003357.01	0.862778	3.981833e-64	1.180151e-60
MsG0180000482.01	MsG0180003821.01	0.835446	1.670600e-56	2.043330e-53
MsG0180000482.01	MsG0180004025.01	0.804101	2.539604e-49	1.298135e-46
MsG0180000482.01	MsG0180004103.01	0.809489	1.845315e-50	1.084884e-47
MsG0180000482.01	MsG0180004106.01	0.803796	2.938041e-49	1.490083e-46
MsG0180000482.01	MsG0180004246.01	0.820625	6.215096e-53	4.942758e-50
MsG0180000482.01	MsG0180004448.01	0.824971	6.043145e-54	5.435858e-51
MsG0180000482.01	MsG0180004493.01	0.837493	5.031764e-57	6.547656e-54
MsG0180000482.01	MsG0180004725.01	0.801154	1.029366e-48	4.880957e-46
MsG0180000482.01	MsG0180005220.01	0.805451	1.326452e-49	7.020049e-47
MsG0180000482.01	MsG0180005240.01	0.811408	7.107977e-51	4.396488e-48
MsG0180000482.01	MsG0180005397.01	0.858721	6.781803e-63	1.747477e-59
MsG0180000482.01	MsG0180005408.01	0.858852	6.199117e-63	1.604603e-59
MsG0180000482.01	MsG0180005418.01	0.845690	3.469195e-59	5.829144e-56
MsG0180000482.01	MsG0180005509.01	0.833781	4.378602e-56	5.094863e-53
MsG0180000482.01	MsG0180005807.01	0.823485	1.350139e-53	1.164068e-50
MsG0180000482.01	MsG0180005913.01	0.800055	1.724869e-48	7.953679e-46
MsG0180000482.01	MsG0180006096.01	0.805404	1.357107e-49	7.173555e-47
MsG0180000482.01	MsG0180006104.01	0.805997	1.018506e-49	5.466674e-47
MsG0180000482.01	MsG0280006360.01	0.834394	3.074492e-56	3.643789e-53
MsG0180000482.01	MsG0280006634.01	0.831745	1.402797e-55	1.536370e-52
MsG0180000482.01	MsG0280006669.01	0.838571	2.657106e-57	3.573404e-54
MsG0180000482.01	MsG0280007062.01	0.831479	1.630988e-55	1.772131e-52
MsG0180000482.01	MsG0280008098.01	0.844032	9.716877e-59	1.549032e-55
MsG0180000482.01	MsG0280008113.01	0.804723	1.884223e-49	9.787408e-47
MsG0180000482.01	MsG0280008419.01	0.865690	4.917754e-65	1.614538e-61
MsG0180000482.01	MsG0280008738.01	0.828916	6.890858e-55	6.944741e-52
MsG0180000482.01	MsG0280008928.01	0.837836	4.107872e-57	5.402365e-54
MsG0180000482.01	MsG0280008929.01	0.864550	1.121451e-64	3.534719e-61
MsG0180000482.01	MsG0280008932.01	0.860837	1.563647e-63	4.332909e-60
MsG0180000482.01	MsG0280009256.01	0.812763	3.599732e-51	2.308389e-48
MsG0180000482.01	MsG0280009350.01	0.809260	2.065566e-50	1.207055e-47
MsG0180000482.01	MsG0280009507.01	0.800002	1.768199e-48	8.142402e-46
MsG0180000482.01	MsG0280009685.01	0.845075	5.089878e-59	8.384510e-56
MsG0180000482.01	MsG0280009708.01	0.824705	6.982215e-54	6.233194e-51
MsG0180000482.01	MsG0280009756.01	0.809061	2.279607e-50	1.324994e-47
MsG0180000482.01	MsG0280010158.01	0.802550	5.319403e-49	2.613118e-46
MsG0180000482.01	MsG0280010181.01	0.813837	2.091012e-51	1.380339e-48
MsG0180000482.01	MsG0280010216.01	0.805171	1.518127e-49	7.976953e-47
MsG0180000482.01	MsG0280010288.01	0.832109	1.140015e-55	1.261799e-52
MsG0180000482.01	MsG0280010517.01	0.811279	7.581800e-51	4.673256e-48
MsG0180000482.01	MsG0280010564.01	0.834800	2.431315e-56	2.916419e-53
MsG0180000482.01	MsG0280011073.01	0.856653	2.780775e-62	6.684089e-59
MsG0180000482.01	MsG0280011401.01	0.822077	2.872932e-53	2.379877e-50
MsG0180000482.01	MsG0380011669.01	0.802010	6.871864e-49	3.329629e-46
MsG0180000482.01	MsG0380012011.01	0.809077	2.261077e-50	1.314822e-47
MsG0180000482.01	MsG0380013342.01	0.831141	1.974487e-55	2.123839e-52
MsG0180000482.01	MsG0380014538.01	0.807163	5.781864e-50	3.197407e-47
MsG0180000482.01	MsG0380014584.01	0.807209	5.651656e-50	3.129275e-47
MsG0180000482.01	MsG0380015745.01	0.815370	9.572175e-52	6.587279e-49
MsG0180000482.01	MsG0380015847.01	-0.843547	1.310310e-58	2.057131e-55
MsG0180000482.01	MsG0380015849.01	-0.819365	1.206650e-52	9.265972e-50
MsG0180000482.01	MsG0380015891.01	0.802466	5.536979e-49	2.714227e-46
MsG0180000482.01	MsG0380015896.01	0.822589	2.184515e-53	1.836561e-50
MsG0180000482.01	MsG0380016182.01	0.809210	2.117995e-50	1.236026e-47
MsG0180000482.01	MsG0380016194.01	0.849915	2.379689e-60	4.582603e-57
MsG0180000482.01	MsG0380016284.01	0.812003	5.273960e-51	3.314104e-48
MsG0180000482.01	MsG0380016546.01	0.808990	2.360043e-50	1.369132e-47
MsG0180000482.01	MsG0380016740.01	0.837561	4.833319e-57	6.302263e-54
MsG0180000482.01	MsG0380017087.01	0.809777	1.599925e-50	9.479137e-48
MsG0180000482.01	MsG0380017338.01	0.833359	5.579932e-56	6.411036e-53
MsG0180000482.01	MsG0380017931.01	0.824020	1.012018e-53	8.857607e-51
MsG0180000482.01	MsG0380018046.01	-0.821338	4.257575e-53	3.454226e-50
MsG0180000482.01	MsG0480018402.01	0.815516	8.880393e-52	6.136079e-49
MsG0180000482.01	MsG0480018847.01	0.837769	4.275453e-57	5.611368e-54
MsG0180000482.01	MsG0480018848.01	0.840837	6.836001e-58	9.858760e-55
MsG0180000482.01	MsG0480018911.01	0.825420	4.733290e-54	4.311987e-51
MsG0180000482.01	MsG0480020024.01	0.812161	4.872152e-51	3.074853e-48
MsG0180000482.01	MsG0480020690.01	0.818053	2.396164e-52	1.774608e-49
MsG0180000482.01	MsG0480020783.01	0.817692	2.889930e-52	2.119302e-49
MsG0180000482.01	MsG0480021468.01	0.800031	1.744233e-48	8.037986e-46
MsG0180000482.01	MsG0480021564.01	0.830672	2.574540e-55	2.731269e-52
MsG0180000482.01	MsG0480022166.01	0.822036	2.936543e-53	2.429688e-50
MsG0180000482.01	MsG0480022178.01	0.805687	1.183638e-49	6.302538e-47
MsG0180000482.01	MsG0480022239.01	0.839102	1.937011e-57	2.647438e-54
MsG0180000482.01	MsG0480022537.01	0.844175	8.894441e-59	1.424221e-55
MsG0180000482.01	MsG0480022624.01	0.831664	1.468639e-55	1.604488e-52
MsG0180000482.01	MsG0480022643.01	0.829246	5.729430e-55	5.831617e-52
MsG0180000482.01	MsG0480022918.01	0.827117	1.867723e-54	1.786219e-51
MsG0180000482.01	MsG0480023261.01	0.803082	4.131648e-49	2.057233e-46
MsG0180000482.01	MsG0480023381.01	0.821480	3.948840e-53	3.216343e-50
MsG0180000482.01	MsG0480023384.01	0.826764	2.268657e-54	2.147392e-51
MsG0180000482.01	MsG0480023867.01	0.815231	1.027591e-51	7.044420e-49
MsG0180000482.01	MsG0480023994.01	0.802075	6.661776e-49	3.233271e-46
MsG0180000482.01	MsG0580024535.01	-0.822622	2.146932e-53	1.806596e-50
MsG0180000482.01	MsG0580024717.01	0.807195	5.690252e-50	3.149452e-47
MsG0180000482.01	MsG0580024775.01	0.839984	1.142725e-57	1.605137e-54
MsG0180000482.01	MsG0580024788.01	0.800419	1.453843e-48	6.766589e-46
MsG0180000482.01	MsG0580024829.01	0.808241	3.412435e-50	1.941138e-47
MsG0180000482.01	MsG0580024873.01	0.803653	3.146278e-49	1.589789e-46
MsG0180000482.01	MsG0580024930.01	-0.833840	4.232362e-56	4.933778e-53
MsG0180000482.01	MsG0580025625.01	0.818932	1.514291e-52	1.149231e-49
MsG0180000482.01	MsG0580025686.01	0.820219	7.700716e-53	6.056134e-50
MsG0180000482.01	MsG0580025906.01	0.832831	7.550427e-56	8.538585e-53
MsG0180000482.01	MsG0580026032.01	0.826842	2.173083e-54	2.061591e-51
MsG0180000482.01	MsG0580026206.01	0.803594	3.235364e-49	1.632460e-46
MsG0180000482.01	MsG0580027725.01	0.802620	5.146428e-49	2.532659e-46
MsG0180000482.01	MsG0580028024.01	0.823828	1.122496e-53	9.772765e-51
MsG0180000482.01	MsG0580028033.01	0.808388	3.173714e-50	1.812367e-47
MsG0180000482.01	MsG0580028059.01	0.804380	2.220663e-49	1.143301e-46
MsG0180000482.01	MsG0580028316.01	0.807598	4.674398e-50	2.614275e-47
MsG0180000482.01	MsG0580028551.01	0.818528	1.870269e-52	1.403489e-49
MsG0180000482.01	MsG0580028918.01	0.801367	9.309113e-49	4.437874e-46
MsG0180000482.01	MsG0580029312.01	0.808989	2.360979e-50	1.369641e-47
MsG0180000482.01	MsG0580029376.01	0.805355	1.389483e-49	7.335543e-47
MsG0180000482.01	MsG0580029414.01	0.860207	2.426138e-63	6.579840e-60
MsG0180000482.01	MsG0580029514.01	-0.816275	6.014180e-52	4.242801e-49
MsG0180000482.01	MsG0580029529.01	0.807670	4.512979e-50	2.528797e-47
MsG0180000482.01	MsG0580029879.01	0.831867	1.308528e-55	1.438327e-52
MsG0180000482.01	MsG0580029912.01	0.828440	8.977833e-55	8.924646e-52
MsG0180000482.01	MsG0680030464.01	0.836174	1.092208e-56	1.365700e-53
MsG0180000482.01	MsG0680030515.01	0.827242	1.743519e-54	1.673661e-51
MsG0180000482.01	MsG0680030680.01	0.813633	2.318953e-51	1.522400e-48
MsG0180000482.01	MsG0680030764.01	0.810700	1.011557e-50	6.140528e-48
MsG0180000482.01	MsG0680031468.01	0.844437	7.566515e-59	1.221432e-55
MsG0180000482.01	MsG0680031469.01	0.852284	5.105430e-61	1.061699e-57
MsG0180000482.01	MsG0680031815.01	0.840672	7.553008e-58	1.083684e-54
MsG0180000482.01	MsG0680032006.01	0.828946	6.775438e-55	6.834354e-52
MsG0180000482.01	MsG0680032413.01	0.803712	3.058916e-49	1.547959e-46
MsG0180000482.01	MsG0680032827.01	0.805104	1.568387e-49	8.226668e-47
MsG0180000482.01	MsG0680032830.01	0.832509	9.077471e-56	1.016770e-52
MsG0180000482.01	MsG0680033377.01	0.819237	1.290583e-52	9.875844e-50
MsG0180000482.01	MsG0680033459.01	0.828642	8.026575e-55	8.025483e-52
MsG0180000482.01	MsG0680034064.01	0.830891	2.274915e-55	2.429055e-52
MsG0180000482.01	MsG0680034235.01	0.845241	4.591535e-59	7.603523e-56
MsG0180000482.01	MsG0680034766.01	0.835020	2.139207e-56	2.583249e-53
MsG0180000482.01	MsG0680034767.01	0.851551	8.243645e-61	1.674682e-57
MsG0180000482.01	MsG0680035307.01	0.871429	6.878596e-67	2.774355e-63
MsG0180000482.01	MsG0680035707.01	0.821199	4.583367e-53	3.704407e-50
MsG0180000482.01	MsG0780036117.01	0.826259	2.992983e-54	2.792583e-51
MsG0180000482.01	MsG0780036263.01	0.810275	1.250048e-50	7.502569e-48
MsG0180000482.01	MsG0780036800.01	0.802049	6.744430e-49	3.271210e-46
MsG0180000482.01	MsG0780036803.01	0.815915	7.238278e-52	5.055837e-49
MsG0180000482.01	MsG0780036880.01	0.820731	5.874658e-53	4.686074e-50
MsG0180000482.01	MsG0780036978.01	0.832377	9.785925e-56	1.091811e-52
MsG0180000482.01	MsG0780037330.01	0.871650	5.814890e-67	2.363855e-63
MsG0180000482.01	MsG0780037331.01	0.804050	2.602018e-49	1.328322e-46
MsG0180000482.01	MsG0780037399.01	0.885053	1.132211e-71	7.654902e-68
MsG0180000482.01	MsG0780037662.01	0.837675	4.518990e-57	5.913875e-54
MsG0180000482.01	MsG0780037701.01	0.828300	9.707888e-55	9.610084e-52
MsG0180000482.01	MsG0780039233.01	0.801389	9.215382e-49	4.395444e-46
MsG0180000482.01	MsG0780039435.01	0.801287	9.670505e-49	4.600719e-46
MsG0180000482.01	MsG0780039439.01	0.805779	1.132053e-49	6.042093e-47
MsG0180000482.01	MsG0780039964.01	0.800902	1.159255e-48	5.461976e-46
MsG0180000482.01	MsG0780039975.01	0.829498	4.976928e-55	5.102523e-52
MsG0180000482.01	MsG0780040374.01	0.829445	5.128530e-55	5.249797e-52
MsG0180000482.01	MsG0780040430.01	0.806108	9.655066e-50	5.196601e-47
MsG0180000482.01	MsG0780041159.01	0.804336	2.268231e-49	1.166438e-46
MsG0180000482.01	MsG0780041187.01	0.803825	2.897532e-49	1.470642e-46
MsG0180000482.01	MsG0780041301.01	0.807790	4.255658e-50	2.392167e-47
MsG0180000482.01	MsG0780041387.01	0.801322	9.510721e-49	4.528689e-46
MsG0180000482.01	MsG0780041482.01	0.822015	2.969806e-53	2.455729e-50
MsG0180000482.01	MsG0780041729.01	-0.847155	1.383153e-59	2.436385e-56
MsG0180000482.01	MsG0780041747.01	0.830788	2.411069e-55	2.566553e-52
MsG0180000482.01	MsG0880042622.01	0.829122	6.140105e-55	6.226276e-52
MsG0180000482.01	MsG0880042857.01	0.828378	9.294932e-55	9.222941e-52
MsG0180000482.01	MsG0880042987.01	-0.815280	1.002159e-51	6.879644e-49
MsG0180000482.01	MsG0880043829.01	0.821658	3.590871e-53	2.939508e-50
MsG0180000482.01	MsG0880044224.01	-0.812728	3.663491e-51	2.347201e-48
MsG0180000482.01	MsG0880044487.01	0.800160	1.641398e-48	7.588802e-46
MsG0180000482.01	MsG0880044538.01	0.810346	1.206416e-50	7.254390e-48
MsG0180000482.01	MsG0880045285.01	0.811666	6.244685e-51	3.888589e-48
MsG0180000482.01	MsG0880045654.01	0.823179	1.592042e-53	1.360819e-50
MsG0180000482.01	MsG0880045992.01	0.815466	9.110864e-52	6.286753e-49
MsG0180000482.01	MsG0880046517.01	0.833702	4.581172e-56	5.317274e-53
MsG0180000482.01	MsG0880046723.01	0.826763	2.269907e-54	2.148494e-51
MsG0180000482.01	MsG0880046736.01	0.805213	1.487635e-49	7.825023e-47
MsG0180000482.01	MsG0880046765.01	0.802406	5.695358e-49	2.787714e-46
MsG0180000482.01	MsG0880047045.01	0.854004	1.642366e-61	3.616183e-58
MsG0180000482.01	MsG0880047046.01	0.876497	1.332462e-68	6.474895e-65
MsG0180000482.01	MsG0880047152.01	0.872413	3.241273e-67	1.355137e-63
MsG0180000482.01	MsG0880047394.01	0.828804	7.331826e-55	7.365319e-52
MsG0180000482.01	MsG0880047519.01	0.803796	2.938041e-49	1.490083e-46
MsG0180000482.01	MsG0880047614.01	0.828278	9.824251e-55	9.719476e-52
MsG0180000482.01	MsG0880047696.01	0.845243	4.587167e-59	7.596678e-56
MsG0180000482.01	MsG0880047703.01	0.815197	1.046105e-51	7.164396e-49

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000482.01.T01	MTR_1g014930	98.658	149	2	0	1	149	1	149	8.60e-108	303
MsG0180000482.01.T01	MTR_3g105340	54.839	124	45	4	13	135	11	124	2.53e-37	124
MsG0180000482.01.T01	MTR_8g021190	46.400	125	54	5	20	136	34	153	4.36e-30	107
MsG0180000482.01.T01	MTR_4g125030	52.727	110	44	3	6	113	15	118	5.64e-30	105
MsG0180000482.01.T01	MTR_4g125010	48.649	111	48	3	6	113	17	121	2.22e-28	102
MsG0180000482.01.T01	MTR_3g109860	37.374	99	58	2	13	110	33	128	1.68e-17	74.3
MsG0180000482.01.T01	MTR_1g103550	45.070	71	37	1	19	89	5	73	2.74e-17	73.2
MsG0180000482.01.T01	MTR_5g006430	39.560	91	49	2	17	107	3	87	3.66e-17	72.8
MsG0180000482.01.T01	MTR_8g013990	31.724	145	82	5	1	136	47	183	6.77e-16	71.2
MsG0180000482.01.T01	MTR_2g101140	45.455	77	31	2	17	86	63	135	1.81e-15	70.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000482.01.T01	AT1G72510	43.939	132	57	5	19	136	36	164	8.25e-30	106
MsG0180000482.01.T01	AT1G72510	43.939	132	57	5	19	136	36	164	8.25e-30	106
MsG0180000482.01.T01	AT5G20670	43.478	138	63	3	11	136	17	151	1.31e-28	103
MsG0180000482.01.T01	AT2G09970	37.931	145	71	6	6	136	26	165	4.33e-24	92.0
MsG0180000482.01.T01	AT1G54095	44.037	109	59	2	1	109	1	107	1.68e-20	82.4
MsG0180000482.01.T01	AT3G22540	37.500	120	59	5	13	131	2	106	6.43e-19	77.0
MsG0180000482.01.T01	AT4G14819	36.134	119	59	4	13	131	2	103	1.24e-17	73.6
MsG0180000482.01.T01	AT2G25780	35.537	121	70	4	20	138	39	153	3.21e-14	66.2
MsG0180000482.01.T01	AT1G79770	70.000	40	12	0	17	56	52	91	1.18e-13	65.1
MsG0180000482.01.T01	AT5G25840	37.008	127	60	6	20	136	46	162	3.14e-13	63.9

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTGAACTGCTGGCGAAATT+TGG	0.269881	1:-6686915	None:intergenic
CCGCAAAATGCAGCTGTTGC+GGG	0.402544	1:+6686732	MsG0180000482.01.T01:CDS
CACGCAGAATGTTCCTTACT+CGG	0.438740	1:-6687022	None:intergenic
TTAGGACTTTGGATTCTCCT+AGG	0.457929	1:+6686993	MsG0180000482.01.T01:CDS
TCCTAGGAGCAAGCCGAGTA+AGG	0.469773	1:+6687009	MsG0180000482.01.T01:CDS
GGGAGAGATTCGGTGGAAGA+TGG	0.469879	1:+6686795	MsG0180000482.01.T01:CDS
GTATGTGTCTCTGTCGTCCT+TGG	0.478341	1:-6687097	None:intergenic
ACCGCAAAATGCAGCTGTTG+CGG	0.482069	1:+6686731	MsG0180000482.01.T01:CDS
CTGAGGCAGTGAAGGAAGAA+AGG	0.486207	1:+6686834	MsG0180000482.01.T01:CDS
AGAAGCTGAAGCGACGGAGA+TGG	0.501388	1:+6686697	MsG0180000482.01.T01:CDS
CATTCTGCGTGCAGACCTCT+TGG	0.504691	1:+6687034	MsG0180000482.01.T01:CDS
AAGCAGAAGATCTGAACTGC+TGG	0.512505	1:-6686925	None:intergenic
GAAGGAAGAAAGGGCGAGGT+CGG	0.514158	1:+6686844	MsG0180000482.01.T01:CDS
ATGCAGCTGTTGCGGGTTGG+TGG	0.517846	1:+6686739	MsG0180000482.01.T01:CDS
TCCTTACTCGGCTTGCTCCT+AGG	0.526193	1:-6687010	None:intergenic
TGTGTCTCTGTCGTCCTTGG+CGG	0.528871	1:-6687094	None:intergenic
GCGCGCGTGAGGGAGAGATT+CGG	0.542153	1:+6686785	MsG0180000482.01.T01:CDS
ATGCGTACATTGCGCGCGTG+AGG	0.557254	1:+6686774	MsG0180000482.01.T01:CDS
TCATAGTTGCTTCTCAACAA+TGG	0.575206	1:+6687063	MsG0180000482.01.T01:CDS
TGCGTACATTGCGCGCGTGA+GGG	0.578384	1:+6686775	MsG0180000482.01.T01:CDS
TGAGGCAGTGAAGGAAGAAA+GGG	0.590842	1:+6686835	MsG0180000482.01.T01:CDS
CAGTGAAGGAAGAAAGGGCG+AGG	0.593016	1:+6686840	MsG0180000482.01.T01:CDS
TTTATGTGCTGAGGCAGTGA+AGG	0.603888	1:+6686826	MsG0180000482.01.T01:CDS
CGCGTGAGGGAGAGATTCGG+TGG	0.607365	1:+6686788	MsG0180000482.01.T01:CDS
TGTGAAAGAAGCTGAAGCGA+CGG	0.619197	1:+6686691	MsG0180000482.01.T01:CDS
AAAATGCAGCTGTTGCGGGT+TGG	0.633011	1:+6686736	MsG0180000482.01.T01:CDS
CAATGGAAACAACTCCGCCA+AGG	0.641855	1:+6687080	MsG0180000482.01.T01:CDS
TTCGGTGGAAGATGGATATG+CGG	0.649354	1:+6686803	MsG0180000482.01.T01:CDS
CAACTATGAGATCTACCAAG+AGG	0.689504	1:-6687049	None:intergenic
CAATTGATGAAGCTTTAATG+CGG	0.691551	1:+6686888	MsG0180000482.01.T01:CDS
GATATGCGGTTTATGTGCTG+AGG	0.715926	1:+6686817	MsG0180000482.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CAATTGATGAAGCTTTAATG+CGG	+	Chr1:6686888-6686907	MsG0180000482.01.T01:CDS	30.0%
!	CTGTGAAACAGATTCTTTTT+AGG	+	Chr1:6686975-6686994	MsG0180000482.01.T01:CDS	30.0%
!!!	ACAGATTCTTTTTAGGACTT+TGG	+	Chr1:6686982-6687001	MsG0180000482.01.T01:CDS	30.0%
	TCATAGTTGCTTCTCAACAA+TGG	+	Chr1:6687063-6687082	MsG0180000482.01.T01:CDS	35.0%
	CAACTATGAGATCTACCAAG+AGG	-	Chr1:6687052-6687071	None:intergenic	40.0%
	TTAGGACTTTGGATTCTCCT+AGG	+	Chr1:6686993-6687012	MsG0180000482.01.T01:CDS	40.0%
	AAGCAGAAGATCTGAACTGC+TGG	-	Chr1:6686928-6686947	None:intergenic	45.0%
	CACGCAGAATGTTCCTTACT+CGG	-	Chr1:6687025-6687044	None:intergenic	45.0%
	GATATGCGGTTTATGTGCTG+AGG	+	Chr1:6686817-6686836	MsG0180000482.01.T01:CDS	45.0%
	TCTGAACTGCTGGCGAAATT+TGG	-	Chr1:6686918-6686937	None:intergenic	45.0%
	TGAGGCAGTGAAGGAAGAAA+GGG	+	Chr1:6686835-6686854	MsG0180000482.01.T01:CDS	45.0%
	TGTGAAAGAAGCTGAAGCGA+CGG	+	Chr1:6686691-6686710	MsG0180000482.01.T01:CDS	45.0%
	TTCGGTGGAAGATGGATATG+CGG	+	Chr1:6686803-6686822	MsG0180000482.01.T01:CDS	45.0%
	TTTATGTGCTGAGGCAGTGA+AGG	+	Chr1:6686826-6686845	MsG0180000482.01.T01:CDS	45.0%
	AAAATGCAGCTGTTGCGGGT+TGG	+	Chr1:6686736-6686755	MsG0180000482.01.T01:CDS	50.0%
	ACCGCAAAATGCAGCTGTTG+CGG	+	Chr1:6686731-6686750	MsG0180000482.01.T01:CDS	50.0%
	CAATGGAAACAACTCCGCCA+AGG	+	Chr1:6687080-6687099	MsG0180000482.01.T01:CDS	50.0%
	CTGAGGCAGTGAAGGAAGAA+AGG	+	Chr1:6686834-6686853	MsG0180000482.01.T01:CDS	50.0%
	GTATGTGTCTCTGTCGTCCT+TGG	-	Chr1:6687100-6687119	None:intergenic	50.0%
	AGAAGCTGAAGCGACGGAGA+TGG	+	Chr1:6686697-6686716	MsG0180000482.01.T01:CDS	55.0%
	CAGTGAAGGAAGAAAGGGCG+AGG	+	Chr1:6686840-6686859	MsG0180000482.01.T01:CDS	55.0%
	CATTCTGCGTGCAGACCTCT+TGG	+	Chr1:6687034-6687053	MsG0180000482.01.T01:CDS	55.0%
	CCGCAAAATGCAGCTGTTGC+GGG	+	Chr1:6686732-6686751	MsG0180000482.01.T01:CDS	55.0%
	GAAGGAAGAAAGGGCGAGGT+CGG	+	Chr1:6686844-6686863	MsG0180000482.01.T01:CDS	55.0%
	TCCTAGGAGCAAGCCGAGTA+AGG	+	Chr1:6687009-6687028	MsG0180000482.01.T01:CDS	55.0%
	TGTGTCTCTGTCGTCCTTGG+CGG	-	Chr1:6687097-6687116	None:intergenic	55.0%
!	CCCGCAACAGCTGCATTTTG+CGG	-	Chr1:6686735-6686754	None:intergenic	55.0%
!	TCCTTACTCGGCTTGCTCCT+AGG	-	Chr1:6687013-6687032	None:intergenic	55.0%
!!	GGGAGAGATTCGGTGGAAGA+TGG	+	Chr1:6686795-6686814	MsG0180000482.01.T01:CDS	55.0%
	ATGCGTACATTGCGCGCGTG+AGG	+	Chr1:6686774-6686793	MsG0180000482.01.T01:CDS	60.0%
	TGCGTACATTGCGCGCGTGA+GGG	+	Chr1:6686775-6686794	MsG0180000482.01.T01:CDS	60.0%
!!	ATGCAGCTGTTGCGGGTTGG+TGG	+	Chr1:6686739-6686758	MsG0180000482.01.T01:CDS	60.0%
	CGCGTGAGGGAGAGATTCGG+TGG	+	Chr1:6686788-6686807	MsG0180000482.01.T01:CDS	65.0%
	GCGCGCGTGAGGGAGAGATT+CGG	+	Chr1:6686785-6686804	MsG0180000482.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	6686677	6687126	6686677	ID=MsG0180000482.01;Name=MsG0180000482.01
Chr1	mRNA	6686677	6687126	6686677	ID=MsG0180000482.01.T01;Parent=MsG0180000482.01;Name=MsG0180000482.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|149
Chr1	exon	6686677	6687126	6686677	ID=MsG0180000482.01.T01:exon:2465;Parent=MsG0180000482.01.T01
Chr1	CDS	6686677	6687126	6686677	ID=MsG0180000482.01.T01:cds;Parent=MsG0180000482.01.T01

Gene Sequence

>MsG0180000482.01.T01

ATGGCTGCGAATGTTGTGAAAGAAGCTGAAGCGACGGAGATGGAAGCTGTTTTGACCGCAAAATGCAGCTGTTGCGGGTTGGTGGAAGAGTGCACACATGCGTACATTGCGCGCGTGAGGGAGAGATTCGGTGGAAGATGGATATGCGGTTTATGTGCTGAGGCAGTGAAGGAAGAAAGGGCGAGGTCGGAGAGTGATGAGAAGATGATCACAATTGATGAAGCTTTAATGCGGCACACCAAATTTCGCCAGCAGTTCAGATCTTCTGCTTCTGATCATAACAAAGATTTCATAGTTGCTGTGAAACAGATTCTTTTTAGGACTTTGGATTCTCCTAGGAGCAAGCCGAGTAAGGAACATTCTGCGTGCAGACCTCTTGGTAGATCTCATAGTTGCTTCTCAACAATGGAAACAACTCCGCCAAGGACGACAGAGACACATACAGAGTGA

Protein sequence

>MsG0180000482.01.T01

MAANVVKEAEATEMEAVLTAKCSCCGLVEECTHAYIARVRERFGGRWICGLCAEAVKEERARSESDEKMITIDEALMRHTKFRQQFRSSASDHNKDFIVAVKQILFRTLDSPRSKPSKEHSACRPLGRSHSCFSTMETTPPRTTETHTE*