Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017053.01.T01 | XP_003602892.1 | 100 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 8.54E-155 | 438 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017053.01.T01 | G7J4K7 | 100.000 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 4.08e-155 | 438 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180004262.01 | MsG0380017053.01 | 0.802389 | 5.742838e-49 | 2.809762e-46 |
| MsG0180006255.01 | MsG0380017053.01 | 0.834449 | 2.977494e-56 | 3.534844e-53 |
| MsG0280006442.01 | MsG0380017053.01 | 0.824103 | 9.678003e-54 | 8.490609e-51 |
| MsG0380017053.01 | MsG0480021697.01 | 0.803638 | 3.167894e-49 | 1.600168e-46 |
| MsG0380017053.01 | MsG0480022638.01 | 0.801189 | 1.012331e-48 | 4.804338e-46 |
| MsG0380017053.01 | MsG0680031009.01 | 0.805909 | 1.063074e-49 | 5.692808e-47 |
| MsG0380017053.01 | MsG0780037917.01 | 0.811316 | 7.441778e-51 | 4.591495e-48 |
| MsG0280006668.01 | MsG0380017053.01 | 0.807146 | 5.829669e-50 | 3.222359e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017053.01.T01 | MTR_3g100100 | 100.000 | 217 | 0 | 0 | 1 | 217 | 1 | 217 | 1.03e-158 | 438 |
| MsG0380017053.01.T01 | MTR_8g068400 | 37.981 | 208 | 119 | 3 | 18 | 220 | 13 | 215 | 9.98e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380017053.01.T01 | AT5G65520 | 41.146 | 192 | 108 | 3 | 18 | 207 | 12 | 200 | 3.90e-40 | 137 |
Find 43 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATACGAAGCGGCGTCTTT+TGG | 0.244593 | 3:-91362673 | None:intergenic |
| CACCAAGCTTTCGCGTTCTT+CGG | 0.307624 | 3:-91362907 | None:intergenic |
| GGAAGCAAAGAAAGCTTATA+AGG | 0.314616 | 3:+91362969 | MsG0380017053.01.T01:CDS |
| GTGTTATGAAGGGAAGAAAA+TGG | 0.346267 | 3:+91362942 | MsG0380017053.01.T01:CDS |
| TTCGTTTGCGAGGTTGTTGA+TGG | 0.363091 | 3:-91362614 | None:intergenic |
| TGCCTGTAGAAGCACTTAAC+AGG | 0.416791 | 3:+91363022 | MsG0380017053.01.T01:CDS |
| GAGGTTGTTGATGGCGGATC+TGG | 0.441588 | 3:-91362605 | None:intergenic |
| GTCCGAAGAACGCGAAAGCT+TGG | 0.448178 | 3:+91362905 | MsG0380017053.01.T01:CDS |
| GCGAAAGCTTGGTGTGCGTT+GGG | 0.450050 | 3:+91362916 | MsG0380017053.01.T01:CDS |
| CGCGAAAGCTTGGTGTGCGT+TGG | 0.456259 | 3:+91362915 | MsG0380017053.01.T01:CDS |
| TTATAAGGAAGCTCATGAAT+TGG | 0.465517 | 3:+91362984 | MsG0380017053.01.T01:CDS |
| TTCACCTTGCTCAAGCGAAC+CGG | 0.466250 | 3:-91362779 | None:intergenic |
| GTAGAAGCACTTAACAGGTT+GGG | 0.475754 | 3:+91363027 | MsG0380017053.01.T01:CDS |
| GCTATGATTCTCGATCTTCA+AGG | 0.478244 | 3:+91362703 | MsG0380017053.01.T01:CDS |
| ACGAGTTGACTCGGCGTTAG+CGG | 0.514403 | 3:+91362858 | MsG0380017053.01.T01:CDS |
| CGCCTAAGGCTTCGTTTGCG+AGG | 0.524088 | 3:-91362624 | None:intergenic |
| TGTAGAAGCACTTAACAGGT+TGG | 0.551808 | 3:+91363026 | MsG0380017053.01.T01:CDS |
| TTAATCTTCCTCAGCATGCA+CGG | 0.553368 | 3:+91362463 | MsG0380017053.01.T01:CDS |
| GCGTTGTCTCCTCTCGCCGC+CGG | 0.558332 | 3:+91362760 | MsG0380017053.01.T01:CDS |
| GCGTTGGGAGAGTGTTATGA+AGG | 0.558916 | 3:+91362931 | MsG0380017053.01.T01:CDS |
| TGAACGCGGACGAGTTGACT+CGG | 0.566518 | 3:+91362849 | MsG0380017053.01.T01:CDS |
| CAAGCGATGTACCGCGACAT+CGG | 0.569247 | 3:+91362409 | MsG0380017053.01.T01:CDS |
| TGAAGGGAAGAAAATGGATG+AGG | 0.575977 | 3:+91362948 | MsG0380017053.01.T01:CDS |
| AACCTGTTAAGTGCTTCTAC+AGG | 0.580757 | 3:-91363024 | None:intergenic |
| GCCGCCGGTTCGCTTGAGCA+AGG | 0.596105 | 3:+91362775 | MsG0380017053.01.T01:CDS |
| TTTGGAATCGGAGTTTAGCG+AGG | 0.596238 | 3:-91362501 | None:intergenic |
| GTTTGCGAGGTTGTTGATGG+CGG | 0.604157 | 3:-91362611 | None:intergenic |
| TCAAGCGAACCGGCGGCGAG+AGG | 0.609088 | 3:-91362769 | None:intergenic |
| ACCTTGCTCAAGCGAACCGG+CGG | 0.627328 | 3:-91362776 | None:intergenic |
| AGTCATCATGATCCTCGCAA+TGG | 0.640456 | 3:+91362438 | MsG0380017053.01.T01:CDS |
| GCAAAGCGTCACTTCGTACG+TGG | 0.647924 | 3:+91362541 | MsG0380017053.01.T01:CDS |
| TCGCTTGAGCAAGGTGAACG+TGG | 0.649304 | 3:+91362784 | MsG0380017053.01.T01:CDS |
| CGTTGGGAGAGTGTTATGAA+GGG | 0.659064 | 3:+91362932 | MsG0380017053.01.T01:CDS |
| GTGGAATTCCGTGCATGCTG+AGG | 0.659142 | 3:-91362471 | None:intergenic |
| AACCTCGCAAACGAAGCCTT+AGG | 0.661102 | 3:+91362622 | MsG0380017053.01.T01:CDS |
| GTTAGCGGATTTGACTGAGT+CGG | 0.668658 | 3:+91362873 | MsG0380017053.01.T01:CDS |
| AGCTTTGAGAAGATACGAAG+CGG | 0.671113 | 3:-91362683 | None:intergenic |
| AAACTCGCAACGAGTGAACG+CGG | 0.681953 | 3:+91362835 | MsG0380017053.01.T01:CDS |
| GGAAGATTAAAGCCATTGCG+AGG | 0.704504 | 3:-91362450 | None:intergenic |
| TGACTTGTAAACCGATGTCG+CGG | 0.717200 | 3:-91362420 | None:intergenic |
| GGCGTCGAGTGATTCAAGAG+CGG | 0.721407 | 3:-91362737 | None:intergenic |
| TCTACAGGCACAGAATACTG+TGG | 0.738815 | 3:-91363009 | None:intergenic |
| GGCGGCGAGAGGAGACAACG+CGG | 0.761382 | 3:-91362758 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TTATAAGGAAGCTCATGAAT+TGG | + | Chr3:91362984-91363003 | MsG0380017053.01.T01:CDS | 30.0% | |
| GGAAGCAAAGAAAGCTTATA+AGG | + | Chr3:91362969-91362988 | MsG0380017053.01.T01:CDS | 35.0% | |
| GTGTTATGAAGGGAAGAAAA+TGG | + | Chr3:91362942-91362961 | MsG0380017053.01.T01:CDS | 35.0% | |
| ! | AGTTTAGCGAGGAATTTTTG+TGG | - | Chr3:91362493-91362512 | None:intergenic | 35.0% |
| !! | CTTTGATTGCTTTTCGGTTT+TGG | - | Chr3:91362522-91362541 | None:intergenic | 35.0% |
| !! | GCTTTGCTTTGATTGCTTTT+CGG | - | Chr3:91362528-91362547 | None:intergenic | 35.0% |
| !!! | TTGCTTTTCGGTTTTGGAAT+CGG | - | Chr3:91362516-91362535 | None:intergenic | 35.0% |
| AACCTGTTAAGTGCTTCTAC+AGG | - | Chr3:91363027-91363046 | None:intergenic | 40.0% | |
| GCTATGATTCTCGATCTTCA+AGG | + | Chr3:91362703-91362722 | MsG0380017053.01.T01:CDS | 40.0% | |
| TGAAGGGAAGAAAATGGATG+AGG | + | Chr3:91362948-91362967 | MsG0380017053.01.T01:CDS | 40.0% | |
| TTAATCTTCCTCAGCATGCA+CGG | + | Chr3:91362463-91362482 | MsG0380017053.01.T01:CDS | 40.0% | |
| !! | AGCTTTGAGAAGATACGAAG+CGG | - | Chr3:91362686-91362705 | None:intergenic | 40.0% |
| !! | GTAGAAGCACTTAACAGGTT+GGG | + | Chr3:91363027-91363046 | MsG0380017053.01.T01:CDS | 40.0% |
| !! | TGTAGAAGCACTTAACAGGT+TGG | + | Chr3:91363026-91363045 | MsG0380017053.01.T01:CDS | 40.0% |
| !!! | ATCGAGTTCGATCGCTTTTT+CGG | - | Chr3:91362653-91362672 | None:intergenic | 40.0% |
| AGTCATCATGATCCTCGCAA+TGG | + | Chr3:91362438-91362457 | MsG0380017053.01.T01:CDS | 45.0% | |
| GGAAGATTAAAGCCATTGCG+AGG | - | Chr3:91362453-91362472 | None:intergenic | 45.0% | |
| TCTACAGGCACAGAATACTG+TGG | - | Chr3:91363012-91363031 | None:intergenic | 45.0% | |
| TTCGTTTGCGAGGTTGTTGA+TGG | - | Chr3:91362617-91362636 | None:intergenic | 45.0% | |
| ! | TGACTTGTAAACCGATGTCG+CGG | - | Chr3:91362423-91362442 | None:intergenic | 45.0% |
| ! | TTTGGAATCGGAGTTTAGCG+AGG | - | Chr3:91362504-91362523 | None:intergenic | 45.0% |
| !! | CGTTGGGAGAGTGTTATGAA+GGG | + | Chr3:91362932-91362951 | MsG0380017053.01.T01:CDS | 45.0% |
| !! | GGCGGATCTGGATTTGTTTT+TGG | - | Chr3:91362596-91362615 | None:intergenic | 45.0% |
| !! | GTTAGCGGATTTGACTGAGT+CGG | + | Chr3:91362873-91362892 | MsG0380017053.01.T01:CDS | 45.0% |
| !! | TGCCTGTAGAAGCACTTAAC+AGG | + | Chr3:91363022-91363041 | MsG0380017053.01.T01:CDS | 45.0% |
| AAACTCGCAACGAGTGAACG+CGG | + | Chr3:91362835-91362854 | MsG0380017053.01.T01:CDS | 50.0% | |
| AACCTCGCAAACGAAGCCTT+AGG | + | Chr3:91362622-91362641 | MsG0380017053.01.T01:CDS | 50.0% | |
| CACCAAGCTTTCGCGTTCTT+CGG | - | Chr3:91362910-91362929 | None:intergenic | 50.0% | |
| TTCACCTTGCTCAAGCGAAC+CGG | - | Chr3:91362782-91362801 | None:intergenic | 50.0% | |
| ! | AGATACGAAGCGGCGTCTTT+TGG | - | Chr3:91362676-91362695 | None:intergenic | 50.0% |
| ! | GTTTGCGAGGTTGTTGATGG+CGG | - | Chr3:91362614-91362633 | None:intergenic | 50.0% |
| !! | GCGTTGGGAGAGTGTTATGA+AGG | + | Chr3:91362931-91362950 | MsG0380017053.01.T01:CDS | 50.0% |
| CAAGCGATGTACCGCGACAT+CGG | + | Chr3:91362409-91362428 | MsG0380017053.01.T01:CDS | 55.0% | |
| GTCCGAAGAACGCGAAAGCT+TGG | + | Chr3:91362905-91362924 | MsG0380017053.01.T01:CDS | 55.0% | |
| GTGGAATTCCGTGCATGCTG+AGG | - | Chr3:91362474-91362493 | None:intergenic | 55.0% | |
| TCGCTTGAGCAAGGTGAACG+TGG | + | Chr3:91362784-91362803 | MsG0380017053.01.T01:CDS | 55.0% | |
| ! | GCAAAGCGTCACTTCGTACG+TGG | + | Chr3:91362541-91362560 | MsG0380017053.01.T01:CDS | 55.0% |
| ! | GGCGTCGAGTGATTCAAGAG+CGG | - | Chr3:91362740-91362759 | None:intergenic | 55.0% |
| ! | TGAACGCGGACGAGTTGACT+CGG | + | Chr3:91362849-91362868 | MsG0380017053.01.T01:CDS | 55.0% |
| !! | ACGAGTTGACTCGGCGTTAG+CGG | + | Chr3:91362858-91362877 | MsG0380017053.01.T01:CDS | 55.0% |
| !! | GAGGTTGTTGATGGCGGATC+TGG | - | Chr3:91362608-91362627 | None:intergenic | 55.0% |
| !! | GCGAAAGCTTGGTGTGCGTT+GGG | + | Chr3:91362916-91362935 | MsG0380017053.01.T01:CDS | 55.0% |
| !!! | CGCTTTTTCGGCTTCGCCTA+AGG | - | Chr3:91362641-91362660 | None:intergenic | 55.0% |
| ACCTTGCTCAAGCGAACCGG+CGG | - | Chr3:91362779-91362798 | None:intergenic | 60.0% | |
| CGCCTAAGGCTTCGTTTGCG+AGG | - | Chr3:91362627-91362646 | None:intergenic | 60.0% | |
| !! | CGCGAAAGCTTGGTGTGCGT+TGG | + | Chr3:91362915-91362934 | MsG0380017053.01.T01:CDS | 60.0% |
| GCGTTGTCTCCTCTCGCCGC+CGG | + | Chr3:91362760-91362779 | MsG0380017053.01.T01:CDS | 70.0% | |
| GGCGGCGAGAGGAGACAACG+CGG | - | Chr3:91362761-91362780 | None:intergenic | 70.0% | |
| TCAAGCGAACCGGCGGCGAG+AGG | - | Chr3:91362772-91362791 | None:intergenic | 70.0% | |
| !! | GCCGCCGGTTCGCTTGAGCA+AGG | + | Chr3:91362775-91362794 | MsG0380017053.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 91362391 | 91363056 | 91362391 | ID=MsG0380017053.01;Name=MsG0380017053.01 |
| Chr3 | mRNA | 91362391 | 91363056 | 91362391 | ID=MsG0380017053.01.T01;Parent=MsG0380017053.01;Name=MsG0380017053.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|221 |
| Chr3 | exon | 91362391 | 91363056 | 91362391 | ID=MsG0380017053.01.T01:exon:12642;Parent=MsG0380017053.01.T01 |
| Chr3 | CDS | 91362391 | 91363056 | 91362391 | ID=MsG0380017053.01.T01:cds;Parent=MsG0380017053.01.T01 |
| Gene Sequence |
| Protein sequence |
