Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019686.01.T01 | PNX79084.1 | 82.407 | 108 | 17 | 1 | 1 | 106 | 1 | 108 | 7.38E-56 | 179 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019686.01.T01 | Q0WWQ1 | 93.023 | 86 | 6 | 0 | 21 | 106 | 197 | 282 | 6.29E-55 | 176 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019686.01.T01 | A0A565CIX0 | 94.186 | 86 | 5 | 0 | 21 | 106 | 197 | 282 | 1.89e-51 | 182 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048687.01 | MsG0480019686.01 | -0.814716 | 1.336939e-51 | 9.038407e-49 |
| MsG0180003793.01 | MsG0480019686.01 | -0.814707 | 1.342964e-51 | 9.076866e-49 |
| MsG0480018477.01 | MsG0480019686.01 | -0.800564 | 1.358638e-48 | 6.346315e-46 |
| MsG0480019686.01 | MsG0480021077.01 | 0.841064 | 5.959224e-58 | 8.653916e-55 |
| MsG0480019686.01 | MsG0480021876.01 | 0.800916 | 1.151175e-48 | 5.425865e-46 |
| MsG0480019686.01 | MsG0580025047.01 | 0.802096 | 6.595830e-49 | 3.202963e-46 |
| MsG0480019686.01 | MsG0580025491.01 | 0.811762 | 5.954049e-51 | 3.717186e-48 |
| MsG0480019686.01 | MsG0780038400.01 | -0.807641 | 4.578075e-50 | 2.563301e-47 |
| MsG0480019686.01 | MsG0780041223.01 | -0.803725 | 3.039514e-49 | 1.538710e-46 |
| MsG0480019686.01 | MsG0880043027.01 | 0.811850 | 5.696689e-51 | 3.564799e-48 |
| MsG0480019686.01 | MsG0880045010.01 | 0.801389 | 9.215878e-49 | 4.395634e-46 |
| MsG0480019686.01 | MsG0880045684.01 | -0.811919 | 5.502216e-51 | 3.449417e-48 |
| MsG0480019686.01 | MsG0880046177.01 | -0.808109 | 3.639565e-50 | 2.063023e-47 |
| MsG0380015118.01 | MsG0480019686.01 | -0.817747 | 2.809636e-52 | 2.063571e-49 |
| MsG0380015598.01 | MsG0480019686.01 | 0.844361 | 7.927361e-59 | 1.276650e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019686.01.T01 | MTR_4g036265 | 95.349 | 86 | 4 | 0 | 21 | 106 | 194 | 279 | 4.20e-57 | 177 |
| MsG0480019686.01.T01 | MTR_4g036265 | 95.349 | 86 | 4 | 0 | 21 | 106 | 194 | 279 | 8.38e-57 | 178 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480019686.01.T01 | AT5G61500 | 93.023 | 86 | 6 | 0 | 21 | 106 | 197 | 282 | 6.41e-56 | 176 |
| MsG0480019686.01.T01 | AT5G61500 | 88.636 | 44 | 5 | 0 | 21 | 64 | 197 | 240 | 7.59e-22 | 87.4 |
Find 22 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACACCCCGTGTGTGGCTTAC+TGG | 0.228881 | 4:+24870075 | MsG0480019686.01.T01:CDS |
| TGAAATTGATATACAATTAC+TGG | 0.330254 | 4:+24869933 | MsG0480019686.01.T01:CDS |
| AGGCTTTACTTGTCAACTTC+AGG | 0.330410 | 4:-24870784 | None:intergenic |
| CATAGAATATTATCATCATC+AGG | 0.409151 | 4:-24869900 | None:intergenic |
| GAGGACCACCCTCATTTGCC+TGG | 0.439384 | 4:+24870679 | MsG0480019686.01.T01:CDS |
| GATCTTGACTAACATCTTCA+AGG | 0.440820 | 4:-24870520 | None:intergenic |
| ACATCTTCAAGGACAAGTTC+TGG | 0.442459 | 4:-24870509 | None:intergenic |
| GCATGTTTCCCAGGCAAATG+AGG | 0.480851 | 4:-24870688 | None:intergenic |
| TGAATAGATGCATGTTTCCC+AGG | 0.493436 | 4:-24870697 | None:intergenic |
| TCAAGATCATGCACGCAAAA+CGG | 0.542906 | 4:+24870535 | MsG0480019686.01.T01:CDS |
| AGGACCACCCTCATTTGCCT+GGG | 0.550927 | 4:+24870680 | MsG0480019686.01.T01:CDS |
| AATGTTGCAGGTAACAATTG+AGG | 0.552648 | 4:+24870660 | MsG0480019686.01.T01:intron |
| ATCACTGCTCCATGTCGACA+TGG | 0.570809 | 4:-24870721 | None:intergenic |
| CATATCCAGTAAGCCACACA+CGG | 0.592594 | 4:-24870080 | None:intergenic |
| CATGTTTCCCAGGCAAATGA+GGG | 0.592997 | 4:-24870687 | None:intergenic |
| ATTATCAAACACCCCGTGTG+TGG | 0.615955 | 4:+24870067 | MsG0480019686.01.T01:CDS |
| TCTATTCATCCATGTCGACA+TGG | 0.639646 | 4:+24870712 | MsG0480019686.01.T01:CDS |
| GTTTCCCAGGCAAATGAGGG+TGG | 0.653245 | 4:-24870684 | None:intergenic |
| ATATCCAGTAAGCCACACAC+GGG | 0.653755 | 4:-24870079 | None:intergenic |
| ATTGATGTGTTGATGTCACG+TGG | 0.716814 | 4:+24870754 | MsG0480019686.01.T01:CDS |
| GTGGCTTACTGGATATGACG+AGG | 0.733325 | 4:+24870086 | MsG0480019686.01.T01:CDS |
| TATCCAGTAAGCCACACACG+GGG | 0.761908 | 4:-24870078 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATTCTCAATTATTATGTT+TGG | - | Chr4:24870359-24870378 | None:intergenic | 15.0% |
| !!! | CTTTTATAATTTAGGTTAAA+TGG | + | Chr4:24870394-24870413 | MsG0480019686.01.T01:intron | 15.0% |
| !! | ATTCTAAATTAAAATGTTGC+AGG | + | Chr4:24870648-24870667 | MsG0480019686.01.T01:intron | 20.0% |
| !! | TAATACATATGTTATGAATC+CGG | - | Chr4:24870257-24870276 | None:intergenic | 20.0% |
| !! | TATAGCATATCAATTGATTA+TGG | + | Chr4:24870563-24870582 | MsG0480019686.01.T01:intron | 20.0% |
| !! | TGAAATTGATATACAATTAC+TGG | + | Chr4:24869933-24869952 | MsG0480019686.01.T01:CDS | 20.0% |
| !!! | ATTCTTATTTTGATGAATAC+CGG | + | Chr4:24870276-24870295 | MsG0480019686.01.T01:intron | 20.0% |
| !!! | GTCAGAAACTTTTTTTTTTT+TGG | - | Chr4:24870329-24870348 | None:intergenic | 20.0% |
| ! | AAAGACTGATTATGATTACA+AGG | - | Chr4:24870131-24870150 | None:intergenic | 25.0% |
| ! | CATAGAATATTATCATCATC+AGG | - | Chr4:24869903-24869922 | None:intergenic | 25.0% |
| ! | TAGAGTTTCAAATAACTGAA+GGG | - | Chr4:24870436-24870455 | None:intergenic | 25.0% |
| ! | TCATATCTGCATAAAAAACA+AGG | - | Chr4:24870042-24870061 | None:intergenic | 25.0% |
| !! | ACTCGATGCTTTTATAATTT+AGG | + | Chr4:24870386-24870405 | MsG0480019686.01.T01:intron | 25.0% |
| !! | TTGATAAGCAATTATTTCTC+TGG | + | Chr4:24869996-24870015 | MsG0480019686.01.T01:intron | 25.0% |
| GTAGAGTTTCAAATAACTGA+AGG | - | Chr4:24870437-24870456 | None:intergenic | 30.0% | |
| TCTCTGGCAATGAATTTAAA+AGG | + | Chr4:24870012-24870031 | MsG0480019686.01.T01:intron | 30.0% | |
| TCTTTCTTGACTCACTTATA+TGG | + | Chr4:24870146-24870165 | MsG0480019686.01.T01:intron | 30.0% | |
| ! | AATTTTCCAGCTAGATACAT+TGG | - | Chr4:24870233-24870252 | None:intergenic | 30.0% |
| ! | ATTTTCCAGCTAGATACATT+GGG | - | Chr4:24870232-24870251 | None:intergenic | 30.0% |
| ! | TCAGTTATTTGAAACTCTAC+TGG | + | Chr4:24870437-24870456 | MsG0480019686.01.T01:intron | 30.0% |
| AATGTTGCAGGTAACAATTG+AGG | + | Chr4:24870660-24870679 | MsG0480019686.01.T01:intron | 35.0% | |
| CTGGCAATGAATTTAAAAGG+TGG | + | Chr4:24870015-24870034 | MsG0480019686.01.T01:intron | 35.0% | |
| GATCTTGACTAACATCTTCA+AGG | - | Chr4:24870523-24870542 | None:intergenic | 35.0% | |
| GTATCTAGCTGGAAAATTAC+CGG | + | Chr4:24870235-24870254 | MsG0480019686.01.T01:intron | 35.0% | |
| TGATTTCATCTCTTGTCTTG+TGG | + | Chr4:24870465-24870484 | MsG0480019686.01.T01:intron | 35.0% | |
| ACATCTTCAAGGACAAGTTC+TGG | - | Chr4:24870512-24870531 | None:intergenic | 40.0% | |
| CATAACCCAATGTATCTAGC+TGG | + | Chr4:24870224-24870243 | MsG0480019686.01.T01:intron | 40.0% | |
| TCAAGATCATGCACGCAAAA+CGG | + | Chr4:24870535-24870554 | MsG0480019686.01.T01:CDS | 40.0% | |
| TCTATTCATCCATGTCGACA+TGG | + | Chr4:24870712-24870731 | MsG0480019686.01.T01:CDS | 40.0% | |
| TGAATAGATGCATGTTTCCC+AGG | - | Chr4:24870700-24870719 | None:intergenic | 40.0% | |
| TTATATGGCTATCGCAAAGC+TGG | + | Chr4:24870161-24870180 | MsG0480019686.01.T01:intron | 40.0% | |
| ! | ACCCATTGATGAAAAGCACA+GGG | + | Chr4:24870299-24870318 | MsG0480019686.01.T01:intron | 40.0% |
| ! | ATTGATGTGTTGATGTCACG+TGG | + | Chr4:24870754-24870773 | MsG0480019686.01.T01:CDS | 40.0% |
| ! | TCCCTGTGCTTTTCATCAAT+GGG | - | Chr4:24870303-24870322 | None:intergenic | 40.0% |
| ATATCCAGTAAGCCACACAC+GGG | - | Chr4:24870082-24870101 | None:intergenic | 45.0% | |
| ATTATCAAACACCCCGTGTG+TGG | + | Chr4:24870067-24870086 | MsG0480019686.01.T01:CDS | 45.0% | |
| CATATCCAGTAAGCCACACA+CGG | - | Chr4:24870083-24870102 | None:intergenic | 45.0% | |
| CATGTTTCCCAGGCAAATGA+GGG | - | Chr4:24870690-24870709 | None:intergenic | 45.0% | |
| ! | GACCCATTGATGAAAAGCAC+AGG | + | Chr4:24870298-24870317 | MsG0480019686.01.T01:intron | 45.0% |
| ! | GTCCCTGTGCTTTTCATCAA+TGG | - | Chr4:24870304-24870323 | None:intergenic | 45.0% |
| ! | GTGCTTTTCATCAATGGGTC+CGG | - | Chr4:24870298-24870317 | None:intergenic | 45.0% |
| ATCACTGCTCCATGTCGACA+TGG | - | Chr4:24870724-24870743 | None:intergenic | 50.0% | |
| GCATGTTTCCCAGGCAAATG+AGG | - | Chr4:24870691-24870710 | None:intergenic | 50.0% | |
| GTGGCTTACTGGATATGACG+AGG | + | Chr4:24870086-24870105 | MsG0480019686.01.T01:CDS | 50.0% | |
| TATCCAGTAAGCCACACACG+GGG | - | Chr4:24870081-24870100 | None:intergenic | 50.0% | |
| AGGACCACCCTCATTTGCCT+GGG | + | Chr4:24870680-24870699 | MsG0480019686.01.T01:CDS | 55.0% | |
| GTTTCCCAGGCAAATGAGGG+TGG | - | Chr4:24870687-24870706 | None:intergenic | 55.0% | |
| ACACCCCGTGTGTGGCTTAC+TGG | + | Chr4:24870075-24870094 | MsG0480019686.01.T01:CDS | 60.0% | |
| GAGGACCACCCTCATTTGCC+TGG | + | Chr4:24870679-24870698 | MsG0480019686.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 24869895 | 24870801 | 24869895 | ID=MsG0480019686.01;Name=MsG0480019686.01 |
| Chr4 | mRNA | 24869895 | 24870801 | 24869895 | ID=MsG0480019686.01.T01;Parent=MsG0480019686.01;Name=MsG0480019686.01.T01;_AED=0.50;_eAED=0.62;_QI=0|0.66|0.5|1|1|1|4|0|106 |
| Chr4 | exon | 24869895 | 24869965 | 24869895 | ID=MsG0480019686.01.T01:exon:4677;Parent=MsG0480019686.01.T01 |
| Chr4 | exon | 24870056 | 24870107 | 24870056 | ID=MsG0480019686.01.T01:exon:4678;Parent=MsG0480019686.01.T01 |
| Chr4 | exon | 24870491 | 24870556 | 24870491 | ID=MsG0480019686.01.T01:exon:4679;Parent=MsG0480019686.01.T01 |
| Chr4 | exon | 24870670 | 24870801 | 24870670 | ID=MsG0480019686.01.T01:exon:4680;Parent=MsG0480019686.01.T01 |
| Chr4 | CDS | 24869895 | 24869965 | 24869895 | ID=MsG0480019686.01.T01:cds;Parent=MsG0480019686.01.T01 |
| Chr4 | CDS | 24870056 | 24870107 | 24870056 | ID=MsG0480019686.01.T01:cds;Parent=MsG0480019686.01.T01 |
| Chr4 | CDS | 24870491 | 24870556 | 24870491 | ID=MsG0480019686.01.T01:cds;Parent=MsG0480019686.01.T01 |
| Chr4 | CDS | 24870670 | 24870801 | 24870670 | ID=MsG0480019686.01.T01:cds;Parent=MsG0480019686.01.T01 |
| Gene Sequence |
| Protein sequence |
