AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880045684.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045684.01.T01 MTR_8g076780 69.048 126 13 1 1 100 70 195 2.05e-46 147
MsG0880045684.01.T01 MTR_2g031760 66.129 62 21 0 39 100 129 190 5.72e-18 74.7
MsG0880045684.01.T01 MTR_2g031960 71.698 53 15 0 48 100 103 155 1.36e-17 73.2
MsG0880045684.01.T01 MTR_7g053520 64.407 59 21 0 42 100 159 217 2.01e-16 71.2
MsG0880045684.01.T01 MTR_2g064300 61.017 59 23 0 42 100 158 216 4.14e-16 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880045684.01.T01 AT4G36420 51.220 123 33 3 1 100 61 179 7.75e-29 102
MsG0880045684.01.T01 AT3G06040 75.472 53 13 0 48 100 134 186 8.41e-18 74.3
MsG0880045684.01.T01 AT3G06040 75.472 53 13 0 48 100 134 186 8.41e-18 74.3
MsG0880045684.01.T01 AT3G06040 75.472 53 13 0 48 100 134 186 8.41e-18 74.3
MsG0880045684.01.T01 AT4G37660 59.677 62 25 0 39 100 106 167 4.29e-16 69.7
MsG0880045684.01.T01 AT1G70190 64.151 53 19 0 48 100 156 208 1.48e-14 66.2
MsG0880045684.01.T01 AT1G70190 64.151 53 19 0 48 100 156 208 1.48e-14 66.2

Find 34 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTGATGCGAGTGAAAA+AGG 0.246427 8:-62693219 MsG0880045684.01.T01:CDS
TGGTTCCTGGAATGGGAATA+AGG 0.260154 8:-62693166 MsG0880045684.01.T01:CDS
AGGTGTAGGTGGAGGGAAAG+GGG 0.337630 8:-62693132 MsG0880045684.01.T01:CDS
TTAAGGTTATTAAAGAAGTT+AGG 0.362138 8:-62693017 MsG0880045684.01.T01:intron
GGTTGGTGCAAAAGCTTCTA+TGG 0.362512 8:-62692878 MsG0880045684.01.T01:CDS
GGTTCCTGGAATGGGAATAA+GGG 0.368867 8:-62693165 MsG0880045684.01.T01:CDS
ATTCCCCTTATTCCCATTCC+AGG 0.371516 8:+62693161 None:intergenic
ATTATGATGCTGATGGTTCC+TGG 0.396685 8:-62693179 MsG0880045684.01.T01:CDS
GGTGTAGGTGGAGGGAAAGG+GGG 0.396694 8:-62693131 MsG0880045684.01.T01:CDS
AGAGGTGTAGGTGGAGGGAA+AGG 0.431861 8:-62693134 MsG0880045684.01.T01:CDS
GTTGAAGGAAGCTAAGGATT+TGG 0.435359 8:-62692977 MsG0880045684.01.T01:CDS
GGAACCATCAGCATCATAAT+CGG 0.467185 8:+62693182 None:intergenic
GGGGATTATTGCTAAGTTGA+AGG 0.470094 8:-62692902 MsG0880045684.01.T01:CDS
GAGGTGTAGGTGGAGGGAAA+GGG 0.479166 8:-62693133 MsG0880045684.01.T01:CDS
ATGCCGAGAGGTGTAGGTGG+AGG 0.492930 8:-62693140 MsG0880045684.01.T01:CDS
ATTGCTAAGTTGAAGGAGGT+TGG 0.495872 8:-62692895 MsG0880045684.01.T01:CDS
ATGCTGATGGTTCCTGGAAT+GGG 0.503308 8:-62693173 MsG0880045684.01.T01:CDS
TTTGGGGTTGAAGGAAGCTA+AGG 0.515112 8:-62692983 MsG0880045684.01.T01:CDS
GATGCTGATGGTTCCTGGAA+TGG 0.517030 8:-62693174 MsG0880045684.01.T01:CDS
TGTGACGAAAGAAGAGGCCG+AGG 0.536944 8:-62692923 MsG0880045684.01.T01:CDS
GATTATTGCTAAGTTGAAGG+AGG 0.540833 8:-62692899 MsG0880045684.01.T01:CDS
AGGGGAATGCCGAGAGGTGT+AGG 0.541839 8:-62693146 MsG0880045684.01.T01:CDS
GAAGGAAGCTAAGGATTTGG+TGG 0.548600 8:-62692974 MsG0880045684.01.T01:CDS
CAACTTAGCAATAATCCCCT+CGG 0.570601 8:+62692906 None:intergenic
TTCCCTCCACCTACACCTCT+CGG 0.576289 8:+62693137 None:intergenic
TGCCGAGAGGTGTAGGTGGA+GGG 0.581642 8:-62693139 MsG0880045684.01.T01:CDS
GGAATGCCGAGAGGTGTAGG+TGG 0.612707 8:-62693143 MsG0880045684.01.T01:CDS
GCTTCCGATTATGATGCTGA+TGG 0.621281 8:-62693186 MsG0880045684.01.T01:CDS
ACACCTTTCTTCAAAAGCGT+AGG 0.629486 8:+62692943 None:intergenic
GTGACGAAAGAAGAGGCCGA+GGG 0.636034 8:-62692922 MsG0880045684.01.T01:CDS
GTTCCTGGAATGGGAATAAG+GGG 0.637463 8:-62693164 MsG0880045684.01.T01:CDS
GAAAGGTGTGACGAAAGAAG+AGG 0.651585 8:-62692929 MsG0880045684.01.T01:CDS
TGACGAAAGAAGAGGCCGAG+GGG 0.703464 8:-62692921 MsG0880045684.01.T01:CDS
GGAATAAGGGGAATGCCGAG+AGG 0.729602 8:-62693152 MsG0880045684.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAGGTTATTAAAGAAGTT+AGG - Chr8:62693086-62693105 MsG0880045684.01.T01:intron 20.0%
! AGTTAGGAGTTTTACTAGTT+TGG - Chr8:62693102-62693121 MsG0880045684.01.T01:intron 30.0%
!! GTTAGGAGTTTTACTAGTTT+GGG - Chr8:62693103-62693122 MsG0880045684.01.T01:intron 30.0%
!! TTAGGAGTTTTACTAGTTTG+GGG - Chr8:62693104-62693123 MsG0880045684.01.T01:intron 30.0%
! GATTATTGCTAAGTTGAAGG+AGG - Chr8:62693204-62693223 MsG0880045684.01.T01:CDS 35.0%
!! TTTTACTAGTTTGGGGTTGA+AGG - Chr8:62693111-62693130 MsG0880045684.01.T01:intron 35.0%
ATTATGATGCTGATGGTTCC+TGG - Chr8:62692924-62692943 MsG0880045684.01.T01:CDS 40.0%
CAACTTAGCAATAATCCCCT+CGG + Chr8:62693200-62693219 None:intergenic 40.0%
GAAGTGATGCGAGTGAAAAA+GGG - Chr8:62692885-62692904 MsG0880045684.01.T01:CDS 40.0%
GGAACCATCAGCATCATAAT+CGG + Chr8:62692924-62692943 None:intergenic 40.0%
GTTGAAGGAAGCTAAGGATT+TGG - Chr8:62693126-62693145 MsG0880045684.01.T01:CDS 40.0%
TGAAGTGATGCGAGTGAAAA+AGG - Chr8:62692884-62692903 MsG0880045684.01.T01:CDS 40.0%
! ACACCTTTCTTCAAAAGCGT+AGG + Chr8:62693163-62693182 None:intergenic 40.0%
! ATTGCTAAGTTGAAGGAGGT+TGG - Chr8:62693208-62693227 MsG0880045684.01.T01:CDS 40.0%
! GGGGATTATTGCTAAGTTGA+AGG - Chr8:62693201-62693220 MsG0880045684.01.T01:CDS 40.0%
!!! GAAGCTTGATGGTTTTGATG+CGG - Chr8:62693054-62693073 MsG0880045684.01.T01:intron 40.0%
ATGCTGATGGTTCCTGGAAT+GGG - Chr8:62692930-62692949 MsG0880045684.01.T01:CDS 45.0%
ATTCCCCTTATTCCCATTCC+AGG + Chr8:62692945-62692964 None:intergenic 45.0%
GAAGGAAGCTAAGGATTTGG+TGG - Chr8:62693129-62693148 MsG0880045684.01.T01:CDS 45.0%
GCGTTTGATGTGAAGCTTGA+TGG - Chr8:62693043-62693062 MsG0880045684.01.T01:intron 45.0%
GCTTCCGATTATGATGCTGA+TGG - Chr8:62692917-62692936 MsG0880045684.01.T01:CDS 45.0%
GGTTCCTGGAATGGGAATAA+GGG - Chr8:62692938-62692957 MsG0880045684.01.T01:CDS 45.0%
GTTCCTGGAATGGGAATAAG+GGG - Chr8:62692939-62692958 MsG0880045684.01.T01:CDS 45.0%
TGATGCGGCGTCAAAGATTA+AGG - Chr8:62693069-62693088 MsG0880045684.01.T01:intron 45.0%
TGGTTCCTGGAATGGGAATA+AGG - Chr8:62692937-62692956 MsG0880045684.01.T01:CDS 45.0%
! GAAAGGTGTGACGAAAGAAG+AGG - Chr8:62693174-62693193 MsG0880045684.01.T01:CDS 45.0%
! GGTTGGTGCAAAAGCTTCTA+TGG - Chr8:62693225-62693244 MsG0880045684.01.T01:CDS 45.0%
! TTTGGGGTTGAAGGAAGCTA+AGG - Chr8:62693120-62693139 MsG0880045684.01.T01:CDS 45.0%
!! TCGCCTACGCTTTTGAAGAA+AGG - Chr8:62693157-62693176 MsG0880045684.01.T01:CDS 45.0%
GATGCTGATGGTTCCTGGAA+TGG - Chr8:62692929-62692948 MsG0880045684.01.T01:CDS 50.0%
GGGTGAGGTGAAGAAAGTTG+AGG - Chr8:62693012-62693031 MsG0880045684.01.T01:CDS 50.0%
! GGGGTGGTGGTAGTGAAAAA+GGG - Chr8:62692991-62693010 MsG0880045684.01.T01:CDS 50.0%
! GGGTGGTGGTAGTGAAAAAG+GGG - Chr8:62692992-62693011 MsG0880045684.01.T01:CDS 50.0%
! GTGGTAGTGAAAAAGGGGTG+AGG - Chr8:62692997-62693016 MsG0880045684.01.T01:CDS 50.0%
AGGTGTAGGTGGAGGGAAAG+GGG - Chr8:62692971-62692990 MsG0880045684.01.T01:CDS 55.0%
GAGGTGTAGGTGGAGGGAAA+GGG - Chr8:62692970-62692989 MsG0880045684.01.T01:CDS 55.0%
GGAATAAGGGGAATGCCGAG+AGG - Chr8:62692951-62692970 MsG0880045684.01.T01:CDS 55.0%
GTGACGAAAGAAGAGGCCGA+GGG - Chr8:62693181-62693200 MsG0880045684.01.T01:CDS 55.0%
TGACGAAAGAAGAGGCCGAG+GGG - Chr8:62693182-62693201 MsG0880045684.01.T01:CDS 55.0%
TGTGACGAAAGAAGAGGCCG+AGG - Chr8:62693180-62693199 MsG0880045684.01.T01:CDS 55.0%
TTCCCTCCACCTACACCTCT+CGG + Chr8:62692969-62692988 None:intergenic 55.0%
! AGAGGTGTAGGTGGAGGGAA+AGG - Chr8:62692969-62692988 MsG0880045684.01.T01:CDS 55.0%
! GGGGGTGGTGGTAGTGAAAA+AGG - Chr8:62692990-62693009 MsG0880045684.01.T01:CDS 55.0%
GGTGTAGGTGGAGGGAAAGG+GGG - Chr8:62692972-62692991 MsG0880045684.01.T01:CDS 60.0%
! AGGGGAATGCCGAGAGGTGT+AGG - Chr8:62692957-62692976 MsG0880045684.01.T01:CDS 60.0%
! ATGCCGAGAGGTGTAGGTGG+AGG - Chr8:62692963-62692982 MsG0880045684.01.T01:CDS 60.0%
! GGAATGCCGAGAGGTGTAGG+TGG - Chr8:62692960-62692979 MsG0880045684.01.T01:CDS 60.0%
! TGCCGAGAGGTGTAGGTGGA+GGG - Chr8:62692964-62692983 MsG0880045684.01.T01:CDS 60.0%
GTAGGTGGAGGGAAAGGGGG+TGG - Chr8:62692975-62692994 MsG0880045684.01.T01:CDS 65.0%
GGTGGAGGGAAAGGGGGTGG+TGG - Chr8:62692978-62692997 MsG0880045684.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr8 gene 62692873 62693252 62692873 ID=MsG0880045684.01;Name=MsG0880045684.01
Chr8 mRNA 62692873 62693252 62692873 ID=MsG0880045684.01.T01;Parent=MsG0880045684.01;Name=MsG0880045684.01.T01;_AED=0.44;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|100
Chr8 exon 62693112 62693252 62693112 ID=MsG0880045684.01.T01:exon:23988;Parent=MsG0880045684.01.T01
Chr8 exon 62692873 62693034 62692873 ID=MsG0880045684.01.T01:exon:23987;Parent=MsG0880045684.01.T01
Chr8 CDS 62693112 62693252 62693112 ID=MsG0880045684.01.T01:cds;Parent=MsG0880045684.01.T01
Chr8 CDS 62692873 62693034 62692873 ID=MsG0880045684.01.T01:cds;Parent=MsG0880045684.01.T01
Gene Sequence

>MsG0880045684.01.T01

ATGGATCTCGTTGAAGTGATGCGAGTGAAAAAGGGAATCAACGAGCTTCCGATTATGATGCTGATGGTTCCTGGAATGGGAATAAGGGGAATGCCGAGAGGTGTAGGTGGAGGGAAAGGGGGTGGTGGTAGTGAAAAAGGGGTTATTAAAGAAGTTAGGAGTTTTACTAGTTTGGGGTTGAAGGAAGCTAAGGATTTGGTGGAGAAATCGCCTACGCTTTTGAAGAAAGGTGTGACGAAAGAAGAGGCCGAGGGGATTATTGCTAAGTTGAAGGAGGTTGGTGCAAAAGCTTCTATGGAATAA

Protein sequence

>MsG0880045684.01.T01

MDLVEVMRVKKGINELPIMMLMVPGMGIRGMPRGVGGGKGGGGSEKGVIKEVRSFTSLGLKEAKDLVEKSPTLLKKGVTKEEAEGIIAKLKEVGAKASME*