AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020139.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020139.01.T01 MTR_4g046023 95.468 331 12 2 1 329 1 330 0.0 632
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020139.01.T01 AT4G28210 58.209 268 106 2 65 329 84 348 1.65e-105 313

Find 117 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTATTCCGGGAAGGTTTA+AGG 0.141987 4:+34866675 MsG0480020139.01.T01:CDS
CAATTCACTCGTAACCTAAT+TGG 0.154297 4:+34866574 MsG0480020139.01.T01:CDS
ATTGGCTATGCTGCTGTTAA+AGG 0.182599 4:+34868058 MsG0480020139.01.T01:CDS
CAGATATTATTTGGCCTTGC+AGG 0.217364 4:+34868607 MsG0480020139.01.T01:three_prime_UTR
ACACTTCTGGTTGGGATCTA+TGG 0.226000 4:+34868106 MsG0480020139.01.T01:CDS
GGTTGGTCGCTGATACAATT+TGG 0.233791 4:-34868010 None:intergenic
AGTGCACTCCAGATATTATT+TGG 0.261306 4:+34868598 MsG0480020139.01.T01:three_prime_UTR
TGGGAAGGGGAGTGAGAGTT+TGG 0.274877 4:-34866494 None:intergenic
GCTTACGAGTTGCTGCTAAC+TGG 0.283062 4:+34868359 MsG0480020139.01.T01:CDS
ACCACTGTTCTGGACACTTC+TGG 0.293417 4:+34868093 MsG0480020139.01.T01:CDS
TTGACTGTAACTGGTCTTAT+TGG 0.301880 4:+34868040 MsG0480020139.01.T01:CDS
GGCGCAAATCTGAATGGTTT+GGG 0.315029 4:-34866604 None:intergenic
CCAAACTCAGCTTCGTAAAA+TGG 0.329117 4:-34866306 None:intergenic
TCCATTGAGGATTTGATTTA+CGG 0.329692 4:+34867881 MsG0480020139.01.T01:CDS
TTATTTCTGAACCACTGTTC+TGG 0.335171 4:+34868083 MsG0480020139.01.T01:CDS
AACTGGAAGAACGCTGCATT+TGG 0.338126 4:+34867770 MsG0480020139.01.T01:CDS
ATATAATCCTTAAACCTTCC+CGG 0.338476 4:-34866682 None:intergenic
GCATTAGTCTATCGATTCAT+AGG 0.341248 4:+34867833 MsG0480020139.01.T01:CDS
GAAAGAGCCTAGTACTATTC+CGG 0.341796 4:+34866663 MsG0480020139.01.T01:CDS
TTTCTTGCAGCTTTAGCAAT+TGG 0.350316 4:+34868337 MsG0480020139.01.T01:CDS
CTCAGCTTCATCGTTATTTG+TGG 0.363186 4:-34866641 None:intergenic
GGCCACTTGAAAGGAGATTC+AGG 0.364131 4:-34866715 None:intergenic
TTCTATTTGAACTGTCAAAC+TGG 0.364696 4:+34867753 MsG0480020139.01.T01:CDS
TGTTGGTGGTGGCGTGGGAA+GGG 0.369562 4:-34866508 None:intergenic
CTGCAAGGCCAAATAATATC+TGG 0.370454 4:-34868606 None:intergenic
GTTGGTGGTGGCGTGGGAAG+GGG 0.376687 4:-34866507 None:intergenic
CGGCGCAAATCTGAATGGTT+TGG 0.378763 4:-34866605 None:intergenic
AATTCACTCGTAACCTAATT+GGG 0.379046 4:+34866575 MsG0480020139.01.T01:CDS
TGCTGCGGTTGGAGAGCCTT+GGG 0.383435 4:+34868192 MsG0480020139.01.T01:CDS
TACGAAGCTGAGTTTGGGTT+TGG 0.386471 4:+34866312 MsG0480020139.01.T01:five_prime_UTR
TGGTTGTTGGGAATTGTTGT+TGG 0.387214 4:-34866525 None:intergenic
TTGTTGGTGGTGGCGTGGGA+AGG 0.389058 4:-34866509 None:intergenic
GGATTTGATTTACGGCGTTC+AGG 0.392877 4:+34867889 MsG0480020139.01.T01:CDS
TAAAATGTTTAGAGAATCAA+TGG 0.396565 4:+34868645 MsG0480020139.01.T01:three_prime_UTR
GATTTGAAGGTGAGGAGGGA+TGG 0.400189 4:-34866472 None:intergenic
TGTTCTGGACACTTCTGGTT+GGG 0.405383 4:+34868098 MsG0480020139.01.T01:CDS
CTGCTAACTGGGCCGGGTAT+CGG 0.413820 4:+34868371 MsG0480020139.01.T01:CDS
ATTGTTGTTGGTGGTGGCGT+GGG 0.416849 4:-34866513 None:intergenic
AAAGAGCCTAGTACTATTCC+GGG 0.417284 4:+34866664 MsG0480020139.01.T01:CDS
GAGTGAGAGTTTGGATTTGA+AGG 0.418757 4:-34866485 None:intergenic
TTGCTGCGGTTGGAGAGCCT+TGG 0.419677 4:+34868191 MsG0480020139.01.T01:CDS
TGGTGGACATGGTTATGTTA+TGG 0.422634 4:-34866444 None:intergenic
TTCAAGTGGCCCATGTTTGT+AGG 0.427360 4:+34866727 MsG0480020139.01.T01:CDS
ATTTGAAGAAGAATGAAGTT+TGG 0.428938 4:+34868441 MsG0480020139.01.T01:three_prime_UTR
ATTTGAAGGTGAGGAGGGAT+GGG 0.432349 4:-34866471 None:intergenic
AATTGTTGTTGGTGGTGGCG+TGG 0.434278 4:-34866514 None:intergenic
TTGGGAATTGTTGTTGGTGG+TGG 0.434853 4:-34866519 None:intergenic
ATGGTAGTATGACTGCTTAT+AGG 0.436754 4:+34868406 MsG0480020139.01.T01:exon
ACGAGGGTCAGGTCTGGTAC+AGG 0.441955 4:-34867941 None:intergenic
CAGGTCTGGTACAGGGGCAC+TGG 0.444032 4:-34867933 None:intergenic
CTTACGAGTTGCTGCTAACT+GGG 0.446058 4:+34868360 MsG0480020139.01.T01:CDS
TGAACTCATGAAGAACAAAA+TGG 0.448860 4:-34866364 None:intergenic
AATAACAAGCTTGGATGAAA+AGG 0.450265 4:-34868553 None:intergenic
GCAGCAAGTACTGCTGCAAC+AGG 0.450924 4:-34868175 None:intergenic
AGAGCCTTGGGTTAGATTTG+TGG 0.451958 4:+34868204 MsG0480020139.01.T01:CDS
TTTGGATTTGAAGGTGAGGA+GGG 0.451974 4:-34866476 None:intergenic
TTGTTGGGAATTGTTGTTGG+TGG 0.452625 4:-34866522 None:intergenic
GGATGGGATTGTGGTGGACA+TGG 0.456531 4:-34866455 None:intergenic
CGAGTTGCTGCTAACTGGGC+CGG 0.458534 4:+34868364 MsG0480020139.01.T01:CDS
GGTGAGGAGGGATGGGATTG+TGG 0.458615 4:-34866464 None:intergenic
TGAATTGAGGGTTGAAAGGT+AGG 0.461827 4:-34866558 None:intergenic
AATTGCTCAAATAACAAGCT+TGG 0.469512 4:-34868562 None:intergenic
TTGAAAGGAGATTCAGGAGC+TGG 0.472670 4:-34866709 None:intergenic
CCGGGTATCGGCACTTGACA+TGG 0.476196 4:+34868383 MsG0480020139.01.T01:CDS
AGATATTATTTGGCCTTGCA+GGG 0.479346 4:+34868608 MsG0480020139.01.T01:three_prime_UTR
CGAGTGAATTGAGGGTTGAA+AGG 0.484135 4:-34866562 None:intergenic
CCAGTTACAGTCAAAACATA+AGG 0.489870 4:-34868031 None:intergenic
GAAGCTGAGTTTGGGTTTGG+AGG 0.494683 4:+34866315 MsG0480020139.01.T01:five_prime_UTR
TGAAGAAGAATGAAGTTTGG+TGG 0.494696 4:+34868444 MsG0480020139.01.T01:three_prime_UTR
ATTAGGTTACGAGTGAATTG+AGG 0.496319 4:-34866571 None:intergenic
TTAGGTTACGAGTGAATTGA+GGG 0.499074 4:-34866570 None:intergenic
CCAGGACGATAACGAGGGTC+AGG 0.505131 4:-34867952 None:intergenic
TTCACCGGCGCAAATCTGAA+TGG 0.505520 4:-34866610 None:intergenic
CCATGTCAAGTGCCGATACC+CGG 0.509042 4:-34868383 None:intergenic
AAGCTGAGTTTGGGTTTGGA+GGG 0.509844 4:+34866316 MsG0480020139.01.T01:five_prime_UTR
CTGTTCTGGACACTTCTGGT+TGG 0.511960 4:+34868097 MsG0480020139.01.T01:CDS
TTTGGAATCGTTCAATTCGT+AGG 0.513956 4:+34867788 MsG0480020139.01.T01:CDS
CGAGGGTCAGGTCTGGTACA+GGG 0.520023 4:-34867940 None:intergenic
CTCCTGAATCTCCTTTCAAG+TGG 0.520568 4:+34866713 MsG0480020139.01.T01:CDS
AGAGTTTGGATTTGAAGGTG+AGG 0.523478 4:-34866480 None:intergenic
GCCGTAAATCAAATCCTCAA+TGG 0.525248 4:-34867882 None:intergenic
AACCTTCCCGGAATAGTACT+AGG 0.525263 4:-34866670 None:intergenic
GTTTGGATTTGAAGGTGAGG+AGG 0.533506 4:-34866477 None:intergenic
GAGGGTCAGGTCTGGTACAG+GGG 0.535218 4:-34867939 None:intergenic
TTTGTGGCAAAACGCTTCAC+CGG 0.536321 4:-34866625 None:intergenic
TGAAATAGAAGAGGAAGGGA+TGG 0.538180 4:+34868670 MsG0480020139.01.T01:three_prime_UTR
ACAAACATGGGCCACTTGAA+AGG 0.544852 4:-34866724 None:intergenic
ACGATAACGAGGGTCAGGTC+TGG 0.549816 4:-34867947 None:intergenic
ACATAAATTTCAACCCTGCA+AGG 0.551291 4:-34868621 None:intergenic
CCTGACCCTCGTTATCGTCC+TGG 0.557091 4:+34867952 MsG0480020139.01.T01:CDS
GATCCGATTGTAAAAGTGAT+CGG 0.558726 4:-34867860 None:intergenic
GATTGTCTCATACACAGCCT+TGG 0.562416 4:+34868228 MsG0480020139.01.T01:CDS
TGGCATGAAATAGAAGAGGA+AGG 0.571219 4:+34868665 MsG0480020139.01.T01:three_prime_UTR
CAAACCATTCAGATTTGCGC+CGG 0.574577 4:+34866606 MsG0480020139.01.T01:CDS
GAATTGAGGGTTGAAAGGTA+GGG 0.583430 4:-34866557 None:intergenic
GCAGCAGTACTTGCTGCGGT+TGG 0.584378 4:+34868181 MsG0480020139.01.T01:CDS
GAGTTGCTGCTAACTGGGCC+GGG 0.585340 4:+34868365 MsG0480020139.01.T01:CDS
AAGAAACTGACCTACAAACA+TGG 0.591277 4:-34866737 None:intergenic
CTGATGCCAGGACGATAACG+AGG 0.598256 4:-34867958 None:intergenic
GGCATGAAATAGAAGAGGAA+GGG 0.598793 4:+34868666 MsG0480020139.01.T01:three_prime_UTR
ACTGGAAGCTTCCTCCTATG+TGG 0.599614 4:-34868307 None:intergenic
CAGCTAAAACAACTGATGCC+AGG 0.602434 4:-34867970 None:intergenic
TTACAGTCAAAACATAAGGT+TGG 0.605207 4:-34868027 None:intergenic
TGAATGCTGATAAATAGCCA+AGG 0.615256 4:-34868245 None:intergenic
GAGGAGGGATGGGATTGTGG+TGG 0.617809 4:-34866461 None:intergenic
GCGCAAATCTGAATGGTTTG+GGG 0.618087 4:-34866603 None:intergenic
GTATCGGCACTTGACATGGA+TGG 0.621788 4:+34868387 MsG0480020139.01.T01:CDS
AAGATATTGAACCACATAGG+AGG 0.621819 4:+34868296 MsG0480020139.01.T01:CDS
AGAAACTGACCTACAAACAT+GGG 0.633090 4:-34866736 None:intergenic
TCAATGGCATGAAATAGAAG+AGG 0.633408 4:+34868661 MsG0480020139.01.T01:three_prime_UTR
TGTTGCAGCAGTACTTGCTG+CGG 0.639554 4:+34868177 MsG0480020139.01.T01:CDS
ATCACCACAAATCTAACCCA+AGG 0.640703 4:-34868208 None:intergenic
AGCCTAGTACTATTCCGGGA+AGG 0.645406 4:+34866668 MsG0480020139.01.T01:CDS
ACCAGAAGTGTCCAGAACAG+TGG 0.647784 4:-34868094 None:intergenic
CGCAAATCTGAATGGTTTGG+GGG 0.655298 4:-34866602 None:intergenic
TTGAAGATATTGAACCACAT+AGG 0.669977 4:+34868293 MsG0480020139.01.T01:CDS
TGATGCCAGGACGATAACGA+GGG 0.754330 4:-34867957 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAAGTAAACATTCATTT+TGG - Chr4:34867252-34867271 None:intergenic 15.0%
!! ATACTACAAAAATTACTCTA+TGG - Chr4:34867540-34867559 None:intergenic 20.0%
!! ATAGAAAAGTTTAGATGAAT+CGG + Chr4:34866812-34866831 MsG0480020139.01.T01:intron 20.0%
!! ATTCAAAAATGATTTCAGAA+GGG + Chr4:34868263-34868282 MsG0480020139.01.T01:CDS 20.0%
!! TAAAATGTTTAGAGAATCAA+TGG + Chr4:34868645-34868664 MsG0480020139.01.T01:three_prime_UTR 20.0%
!! TGTAATCAAATATTGATGTT+CGG + Chr4:34866786-34866805 MsG0480020139.01.T01:intron 20.0%
!!! ATATGGAACTGAATTTTAAT+AGG - Chr4:34867453-34867472 None:intergenic 20.0%
!!! ATCTTCTTTTAATTACTTCT+TGG - Chr4:34867157-34867176 None:intergenic 20.0%
!!! CAAATATGTATTTGTTAGTT+TGG + Chr4:34867094-34867113 MsG0480020139.01.T01:intron 20.0%
!!! GCTTTTCAAAATTTATCAAA+AGG + Chr4:34868128-34868147 MsG0480020139.01.T01:CDS 20.0%
!!! TTGTTGTTTTTTTTATCACA+AGG + Chr4:34868525-34868544 MsG0480020139.01.T01:three_prime_UTR 20.0%
! ATTTGAAGAAGAATGAAGTT+TGG + Chr4:34868441-34868460 MsG0480020139.01.T01:three_prime_UTR 25.0%
! CATTCAAAAATGATTTCAGA+AGG + Chr4:34868262-34868281 MsG0480020139.01.T01:CDS 25.0%
! CTTACAAAACACTACAAATA+TGG - Chr4:34867470-34867489 None:intergenic 25.0%
! TAGAAAAGTTTAGATGAATC+GGG + Chr4:34866813-34866832 MsG0480020139.01.T01:intron 25.0%
! TATCTCATTCACTTGTTTAA+AGG + Chr4:34867286-34867305 MsG0480020139.01.T01:intron 25.0%
! TGCATAATATATGCAAGATT+CGG + Chr4:34866924-34866943 MsG0480020139.01.T01:intron 25.0%
!! CAGCACATAATGTTTAAAAT+TGG - Chr4:34867389-34867408 None:intergenic 25.0%
!! TGTTTTGTAAGAATGTTTAG+AGG + Chr4:34867479-34867498 MsG0480020139.01.T01:intron 25.0%
AATAACAAGCTTGGATGAAA+AGG - Chr4:34868556-34868575 None:intergenic 30.0%
AATTCACTCGTAACCTAATT+GGG + Chr4:34866575-34866594 MsG0480020139.01.T01:CDS 30.0%
AATTGCTCAAATAACAAGCT+TGG - Chr4:34868565-34868584 None:intergenic 30.0%
ATATAATCCTTAAACCTTCC+CGG - Chr4:34866685-34866704 None:intergenic 30.0%
ATGACAGAAATAAAACAGTC+TGG - Chr4:34867663-34867682 None:intergenic 30.0%
CATAATATATGCAAGATTCG+GGG + Chr4:34866926-34866945 MsG0480020139.01.T01:intron 30.0%
CATTCACTTGTTTAAAGGAT+GGG + Chr4:34867291-34867310 MsG0480020139.01.T01:intron 30.0%
GCATAATATATGCAAGATTC+GGG + Chr4:34866925-34866944 MsG0480020139.01.T01:intron 30.0%
TAACAAATACCAACGAATGT+TGG - Chr4:34867060-34867079 None:intergenic 30.0%
TCATTCACTTGTTTAAAGGA+TGG + Chr4:34867290-34867309 MsG0480020139.01.T01:intron 30.0%
TGAACTCATGAAGAACAAAA+TGG - Chr4:34866367-34866386 None:intergenic 30.0%
TTACAGTCAAAACATAAGGT+TGG - Chr4:34868030-34868049 None:intergenic 30.0%
TTCAAAGACTATAGTGAAAC+CGG + Chr4:34867210-34867229 MsG0480020139.01.T01:intron 30.0%
TTCTATTTGAACTGTCAAAC+TGG + Chr4:34867753-34867772 MsG0480020139.01.T01:CDS 30.0%
TTGAAGATATTGAACCACAT+AGG + Chr4:34868293-34868312 MsG0480020139.01.T01:CDS 30.0%
! AACATGTAAGTACCTCTTTT+TGG - Chr4:34866970-34866989 None:intergenic 30.0%
! TCCATTGAGGATTTGATTTA+CGG + Chr4:34867881-34867900 MsG0480020139.01.T01:CDS 30.0%
!! AATCCGATCACTTTTACAAT+CGG + Chr4:34867857-34867876 MsG0480020139.01.T01:CDS 30.0%
!! TATGGTTACAGTTTTGTCTT+TGG - Chr4:34866429-34866448 None:intergenic 30.0%
!!! AGACTGTTTTATTTCTGTCA+TGG + Chr4:34867662-34867681 MsG0480020139.01.T01:intron 30.0%
AAACAAAAGGGTTTCCACAA+TGG - Chr4:34867638-34867657 None:intergenic 35.0%
AAAGTTTAGATGAATCGGGT+CGG + Chr4:34866817-34866836 MsG0480020139.01.T01:intron 35.0%
AACAAAAGGGTTTCCACAAT+GGG - Chr4:34867637-34867656 None:intergenic 35.0%
AAGAAACTGACCTACAAACA+TGG - Chr4:34866740-34866759 None:intergenic 35.0%
AAGATATTGAACCACATAGG+AGG + Chr4:34868296-34868315 MsG0480020139.01.T01:CDS 35.0%
AATTACTTCTTGGACCAATG+TGG - Chr4:34867147-34867166 None:intergenic 35.0%
ACATAAATTTCAACCCTGCA+AGG - Chr4:34868624-34868643 None:intergenic 35.0%
AGAAACTGACCTACAAACAT+GGG - Chr4:34866739-34866758 None:intergenic 35.0%
AGTGCACTCCAGATATTATT+TGG + Chr4:34868598-34868617 MsG0480020139.01.T01:three_prime_UTR 35.0%
ATGGTAGTATGACTGCTTAT+AGG + Chr4:34868406-34868425 MsG0480020139.01.T01:exon 35.0%
ATTAGGTTACGAGTGAATTG+AGG - Chr4:34866574-34866593 None:intergenic 35.0%
CAATTCACTCGTAACCTAAT+TGG + Chr4:34866574-34866593 MsG0480020139.01.T01:CDS 35.0%
CCAGTTACAGTCAAAACATA+AGG - Chr4:34868034-34868053 None:intergenic 35.0%
GATCCGATTGTAAAAGTGAT+CGG - Chr4:34867863-34867882 None:intergenic 35.0%
TCAATGGCATGAAATAGAAG+AGG + Chr4:34868661-34868680 MsG0480020139.01.T01:three_prime_UTR 35.0%
TGAAGAAGAATGAAGTTTGG+TGG + Chr4:34868444-34868463 MsG0480020139.01.T01:three_prime_UTR 35.0%
TGAATGCTGATAAATAGCCA+AGG - Chr4:34868248-34868267 None:intergenic 35.0%
TTAGGTTACGAGTGAATTGA+GGG - Chr4:34866573-34866592 None:intergenic 35.0%
! ATGTAAGTACCTCTTTTTGG+TGG - Chr4:34866967-34866986 None:intergenic 35.0%
! CATTTTACGAAGCTGAGTTT+GGG + Chr4:34866307-34866326 MsG0480020139.01.T01:five_prime_UTR 35.0%
! TGGCTTTTCTTATCTATGCT+TGG + Chr4:34867723-34867742 MsG0480020139.01.T01:CDS 35.0%
! TTTCTTGCAGCTTTAGCAAT+TGG + Chr4:34868337-34868356 MsG0480020139.01.T01:CDS 35.0%
! TTTGGAATCGTTCAATTCGT+AGG + Chr4:34867788-34867807 MsG0480020139.01.T01:CDS 35.0%
! TTTTACAATCGGATCCATTG+AGG + Chr4:34867868-34867887 MsG0480020139.01.T01:CDS 35.0%
!! AGATATTATTTGGCCTTGCA+GGG + Chr4:34868608-34868627 MsG0480020139.01.T01:three_prime_UTR 35.0%
!! GCATTAGTCTATCGATTCAT+AGG + Chr4:34867833-34867852 MsG0480020139.01.T01:CDS 35.0%
!! TTATTTCTGAACCACTGTTC+TGG + Chr4:34868083-34868102 MsG0480020139.01.T01:CDS 35.0%
!! TTGACTGTAACTGGTCTTAT+TGG + Chr4:34868040-34868059 MsG0480020139.01.T01:CDS 35.0%
!!! CCTTATGTTTTGACTGTAAC+TGG + Chr4:34868031-34868050 MsG0480020139.01.T01:CDS 35.0%
AAACAGTCTGGCGAAACAAA+AGG - Chr4:34867651-34867670 None:intergenic 40.0%
AAAGAGCCTAGTACTATTCC+GGG + Chr4:34866664-34866683 MsG0480020139.01.T01:CDS 40.0%
AACAGTCTGGCGAAACAAAA+GGG - Chr4:34867650-34867669 None:intergenic 40.0%
AGAGTTTGGATTTGAAGGTG+AGG - Chr4:34866483-34866502 None:intergenic 40.0%
ATCACCACAAATCTAACCCA+AGG - Chr4:34868211-34868230 None:intergenic 40.0%
ATTGGCTATGCTGCTGTTAA+AGG + Chr4:34868058-34868077 MsG0480020139.01.T01:CDS 40.0%
CCAAACTCAGCTTCGTAAAA+TGG - Chr4:34866309-34866328 None:intergenic 40.0%
CTCAGCTTCATCGTTATTTG+TGG - Chr4:34866644-34866663 None:intergenic 40.0%
CTGCAAGGCCAAATAATATC+TGG - Chr4:34868609-34868628 None:intergenic 40.0%
GAAAGAGCCTAGTACTATTC+CGG + Chr4:34866663-34866682 MsG0480020139.01.T01:CDS 40.0%
GAATGTTGGTCCAGTTAGTA+CGG - Chr4:34867046-34867065 None:intergenic 40.0%
GAATTGAGGGTTGAAAGGTA+GGG - Chr4:34866560-34866579 None:intergenic 40.0%
GAGTGAGAGTTTGGATTTGA+AGG - Chr4:34866488-34866507 None:intergenic 40.0%
GCCGTAAATCAAATCCTCAA+TGG - Chr4:34867885-34867904 None:intergenic 40.0%
GCTAAAGCTGCAAGAAAAAG+AGG - Chr4:34868334-34868353 None:intergenic 40.0%
GGCATGAAATAGAAGAGGAA+GGG + Chr4:34868666-34868685 MsG0480020139.01.T01:three_prime_UTR 40.0%
TACTATTCCGGGAAGGTTTA+AGG + Chr4:34866675-34866694 MsG0480020139.01.T01:CDS 40.0%
TCAACTAAGCTAAGCTCACA+AGG - Chr4:34866895-34866914 None:intergenic 40.0%
TCTTTGTCAATGTACAGCAC+TGG + Chr4:34867703-34867722 MsG0480020139.01.T01:intron 40.0%
TGAATTGAGGGTTGAAAGGT+AGG - Chr4:34866561-34866580 None:intergenic 40.0%
TGGCATGAAATAGAAGAGGA+AGG + Chr4:34868665-34868684 MsG0480020139.01.T01:three_prime_UTR 40.0%
TGGTCCATAAATATGCAAGC+CGG - Chr4:34867232-34867251 None:intergenic 40.0%
TGGTTGTTGGGAATTGTTGT+TGG - Chr4:34866528-34866547 None:intergenic 40.0%
TTTGGATTTGAAGGTGAGGA+GGG - Chr4:34866479-34866498 None:intergenic 40.0%
! AGTTGACTCATACCACACAA+AGG - Chr4:34867023-34867042 None:intergenic 40.0%
! ATGTTACTTACTGACGAACG+AGG + Chr4:34866985-34867004 MsG0480020139.01.T01:intron 40.0%
! CCATTTTACGAAGCTGAGTT+TGG + Chr4:34866306-34866325 MsG0480020139.01.T01:five_prime_UTR 40.0%
! GTTGAAAGGTAGGGATTTTG+TGG - Chr4:34866551-34866570 None:intergenic 40.0%
! TGGTGGACATGGTTATGTTA+TGG - Chr4:34866447-34866466 None:intergenic 40.0%
! TGGTGGTCTGTAAAGTTTTG+AGG + Chr4:34868461-34868480 MsG0480020139.01.T01:three_prime_UTR 40.0%
! TTGTTGGGAATTGTTGTTGG+TGG - Chr4:34866525-34866544 None:intergenic 40.0%
!! CAGATATTATTTGGCCTTGC+AGG + Chr4:34868607-34868626 MsG0480020139.01.T01:three_prime_UTR 40.0%
!! GAAACCGGCTTGCATATTTA+TGG + Chr4:34867225-34867244 MsG0480020139.01.T01:intron 40.0%
!! TAAGTACCTCTTTTTGGTGG+TGG - Chr4:34866964-34866983 None:intergenic 40.0%
!!! GGTCTGTAAAGTTTTGAGGA+AGG + Chr4:34868465-34868484 MsG0480020139.01.T01:three_prime_UTR 40.0%
AACCTTCCCGGAATAGTACT+AGG - Chr4:34866673-34866692 None:intergenic 45.0%
AACTGGAAGAACGCTGCATT+TGG + Chr4:34867770-34867789 MsG0480020139.01.T01:CDS 45.0%
AATACCCTCAGTTCCCATTG+TGG + Chr4:34867621-34867640 MsG0480020139.01.T01:intron 45.0%
ACAAACATGGGCCACTTGAA+AGG - Chr4:34866727-34866746 None:intergenic 45.0%
ACGGACATAAGACGCAATTG+AGG - Chr4:34867121-34867140 None:intergenic 45.0%
ATTTGAAGGTGAGGAGGGAT+GGG - Chr4:34866474-34866493 None:intergenic 45.0%
CAAACCATTCAGATTTGCGC+CGG + Chr4:34866606-34866625 MsG0480020139.01.T01:CDS 45.0%
CAGCTAAAACAACTGATGCC+AGG - Chr4:34867973-34867992 None:intergenic 45.0%
CGAGTGAATTGAGGGTTGAA+AGG - Chr4:34866565-34866584 None:intergenic 45.0%
CTAACTGGACCAACATTCGT+TGG + Chr4:34867048-34867067 MsG0480020139.01.T01:intron 45.0%
CTCCTGAATCTCCTTTCAAG+TGG + Chr4:34866713-34866732 MsG0480020139.01.T01:CDS 45.0%
CTTACGAGTTGCTGCTAACT+GGG + Chr4:34868360-34868379 MsG0480020139.01.T01:CDS 45.0%
CTTATGTCCGTTAGCCACAT+TGG + Chr4:34867130-34867149 MsG0480020139.01.T01:intron 45.0%
GATTGTCTCATACACAGCCT+TGG + Chr4:34868228-34868247 MsG0480020139.01.T01:CDS 45.0%
GCGCAAATCTGAATGGTTTG+GGG - Chr4:34866606-34866625 None:intergenic 45.0%
GCTGCAAGAAAAAGAGGAAC+TGG - Chr4:34868328-34868347 None:intergenic 45.0%
GGCGCAAATCTGAATGGTTT+GGG - Chr4:34866607-34866626 None:intergenic 45.0%
GTTTCCACAATGGGAACTGA+GGG - Chr4:34867628-34867647 None:intergenic 45.0%
GTTTGGATTTGAAGGTGAGG+AGG - Chr4:34866480-34866499 None:intergenic 45.0%
GTTTGGGGGAAAACCCAATT+AGG - Chr4:34866591-34866610 None:intergenic 45.0%
TCTTGGACCAATGTGGCTAA+CGG - Chr4:34867140-34867159 None:intergenic 45.0%
TGAGTCAACTCCGTACTAAC+TGG + Chr4:34867033-34867052 MsG0480020139.01.T01:intron 45.0%
TTCAAGTGGCCCATGTTTGT+AGG + Chr4:34866727-34866746 MsG0480020139.01.T01:CDS 45.0%
TTGAAAGGAGATTCAGGAGC+TGG - Chr4:34866712-34866731 None:intergenic 45.0%
TTTGTGGCAAAACGCTTCAC+CGG - Chr4:34866628-34866647 None:intergenic 45.0%
! AAGCTGAGTTTGGGTTTGGA+GGG + Chr4:34866316-34866335 MsG0480020139.01.T01:five_prime_UTR 45.0%
! ACACTTCTGGTTGGGATCTA+TGG + Chr4:34868106-34868125 MsG0480020139.01.T01:CDS 45.0%
! AGAGCCTTGGGTTAGATTTG+TGG + Chr4:34868204-34868223 MsG0480020139.01.T01:CDS 45.0%
! AGGGATTTTGTGGTGGTTGT+TGG - Chr4:34866541-34866560 None:intergenic 45.0%
! CGCAAATCTGAATGGTTTGG+GGG - Chr4:34866605-34866624 None:intergenic 45.0%
! GAAAGGTAGGGATTTTGTGG+TGG - Chr4:34866548-34866567 None:intergenic 45.0%
! GCGTTCAGGCTTTTTACTCT+TGG + Chr4:34867903-34867922 MsG0480020139.01.T01:CDS 45.0%
! GGATTTGATTTACGGCGTTC+AGG + Chr4:34867889-34867908 MsG0480020139.01.T01:CDS 45.0%
! GGGATTTTGTGGTGGTTGTT+GGG - Chr4:34866540-34866559 None:intergenic 45.0%
! GGTTGGTCGCTGATACAATT+TGG - Chr4:34868013-34868032 None:intergenic 45.0%
! TACGAAGCTGAGTTTGGGTT+TGG + Chr4:34866312-34866331 MsG0480020139.01.T01:five_prime_UTR 45.0%
! TGTTCTGGACACTTCTGGTT+GGG + Chr4:34868098-34868117 MsG0480020139.01.T01:CDS 45.0%
! TTGGGAATTGTTGTTGGTGG+TGG - Chr4:34866522-34866541 None:intergenic 45.0%
ACTGGAAGCTTCCTCCTATG+TGG - Chr4:34868310-34868329 None:intergenic 50.0%
AGCCTAGTACTATTCCGGGA+AGG + Chr4:34866668-34866687 MsG0480020139.01.T01:CDS 50.0%
CGGCGCAAATCTGAATGGTT+TGG - Chr4:34866608-34866627 None:intergenic 50.0%
GATTTGAAGGTGAGGAGGGA+TGG - Chr4:34866475-34866494 None:intergenic 50.0%
GCTTACGAGTTGCTGCTAAC+TGG + Chr4:34868359-34868378 MsG0480020139.01.T01:CDS 50.0%
GGCCACTTGAAAGGAGATTC+AGG - Chr4:34866718-34866737 None:intergenic 50.0%
GGTTTCCACAATGGGAACTG+AGG - Chr4:34867629-34867648 None:intergenic 50.0%
TAGATGAATCGGGTCGGTCA+GGG + Chr4:34866823-34866842 MsG0480020139.01.T01:intron 50.0%
TCTTAACGCAGGCCTTTGTG+TGG + Chr4:34867008-34867027 MsG0480020139.01.T01:intron 50.0%
TGATGCCAGGACGATAACGA+GGG - Chr4:34867960-34867979 None:intergenic 50.0%
TTAGATGAATCGGGTCGGTC+AGG + Chr4:34866822-34866841 MsG0480020139.01.T01:intron 50.0%
TTCACCGGCGCAAATCTGAA+TGG - Chr4:34866613-34866632 None:intergenic 50.0%
! ACCACTGTTCTGGACACTTC+TGG + Chr4:34868093-34868112 MsG0480020139.01.T01:CDS 50.0%
! CTGTTCTGGACACTTCTGGT+TGG + Chr4:34868097-34868116 MsG0480020139.01.T01:CDS 50.0%
! GAAGCTGAGTTTGGGTTTGG+AGG + Chr4:34866315-34866334 MsG0480020139.01.T01:five_prime_UTR 50.0%
! GTATCGGCACTTGACATGGA+TGG + Chr4:34868387-34868406 MsG0480020139.01.T01:CDS 50.0%
! TGTTGCAGCAGTACTTGCTG+CGG + Chr4:34868177-34868196 MsG0480020139.01.T01:CDS 50.0%
!! AATTGTTGTTGGTGGTGGCG+TGG - Chr4:34866517-34866536 None:intergenic 50.0%
!! ACCAGAAGTGTCCAGAACAG+TGG - Chr4:34868097-34868116 None:intergenic 50.0%
!! ATTGTTGTTGGTGGTGGCGT+GGG - Chr4:34866516-34866535 None:intergenic 50.0%
!! GGCTAAGAGGGTGTTGATAG+AGG + Chr4:34866856-34866875 MsG0480020139.01.T01:intron 50.0%
!! TCAACACCCTCTTAGCCTTG+AGG - Chr4:34866853-34866872 None:intergenic 50.0%
!!! ACCTCTTTTTGGTGGTGGTC+AGG - Chr4:34866959-34866978 None:intergenic 50.0%
!!! CCTCTTTTTGGTGGTGGTCA+GGG - Chr4:34866958-34866977 None:intergenic 50.0%
ACGATAACGAGGGTCAGGTC+TGG - Chr4:34867950-34867969 None:intergenic 55.0%
CCCTGACCACCACCAAAAAG+AGG + Chr4:34866955-34866974 MsG0480020139.01.T01:intron 55.0%
CTGATGCCAGGACGATAACG+AGG - Chr4:34867961-34867980 None:intergenic 55.0%
GACGAACGAGGTCTTAACGC+AGG + Chr4:34866997-34867016 MsG0480020139.01.T01:intron 55.0%
GGCAAGCCTCAAGGCTAAGA+GGG + Chr4:34866844-34866863 MsG0480020139.01.T01:intron 55.0%
TGGGAAGGGGAGTGAGAGTT+TGG - Chr4:34866497-34866516 None:intergenic 55.0%
! GCAGCAAGTACTGCTGCAAC+AGG - Chr4:34868178-34868197 None:intergenic 55.0%
!! CCATGTCAAGTGCCGATACC+CGG - Chr4:34868386-34868405 None:intergenic 55.0%
!! GGATGGGATTGTGGTGGACA+TGG - Chr4:34866458-34866477 None:intergenic 55.0%
ACGAGGGTCAGGTCTGGTAC+AGG - Chr4:34867944-34867963 None:intergenic 60.0%
CCAGGACGATAACGAGGGTC+AGG - Chr4:34867955-34867974 None:intergenic 60.0%
CCTGACCCTCGTTATCGTCC+TGG + Chr4:34867952-34867971 MsG0480020139.01.T01:CDS 60.0%
CGAGGGTCAGGTCTGGTACA+GGG - Chr4:34867943-34867962 None:intergenic 60.0%
CGAGTTGCTGCTAACTGGGC+CGG + Chr4:34868364-34868383 MsG0480020139.01.T01:CDS 60.0%
CTGCTAACTGGGCCGGGTAT+CGG + Chr4:34868371-34868390 MsG0480020139.01.T01:CDS 60.0%
GAGGAGGGATGGGATTGTGG+TGG - Chr4:34866464-34866483 None:intergenic 60.0%
GAGGGTCAGGTCTGGTACAG+GGG - Chr4:34867942-34867961 None:intergenic 60.0%
GAGTTGCTGCTAACTGGGCC+GGG + Chr4:34868365-34868384 MsG0480020139.01.T01:CDS 60.0%
GGGCAAGCCTCAAGGCTAAG+AGG + Chr4:34866843-34866862 MsG0480020139.01.T01:intron 60.0%
GGTGAGGAGGGATGGGATTG+TGG - Chr4:34866467-34866486 None:intergenic 60.0%
TGCTGCGGTTGGAGAGCCTT+GGG + Chr4:34868192-34868211 MsG0480020139.01.T01:CDS 60.0%
TTGCTGCGGTTGGAGAGCCT+TGG + Chr4:34868191-34868210 MsG0480020139.01.T01:CDS 60.0%
! GCAGCAGTACTTGCTGCGGT+TGG + Chr4:34868181-34868200 MsG0480020139.01.T01:CDS 60.0%
!! CCGGGTATCGGCACTTGACA+TGG + Chr4:34868383-34868402 MsG0480020139.01.T01:CDS 60.0%
!! TGTTGGTGGTGGCGTGGGAA+GGG - Chr4:34866511-34866530 None:intergenic 60.0%
!! TTGTTGGTGGTGGCGTGGGA+AGG - Chr4:34866512-34866531 None:intergenic 60.0%
GTCGGTCAGGGCAAGCCTCA+AGG + Chr4:34866835-34866854 MsG0480020139.01.T01:intron 65.0%
! CAGGTCTGGTACAGGGGCAC+TGG - Chr4:34867936-34867955 None:intergenic 65.0%
!! GTTGGTGGTGGCGTGGGAAG+GGG - Chr4:34866510-34866529 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 34866298 34868690 34866298 ID=MsG0480020139.01;Name=MsG0480020139.01
Chr4 mRNA 34866298 34868690 34866298 ID=MsG0480020139.01.T01;Parent=MsG0480020139.01;Name=MsG0480020139.01.T01;_AED=0.42;_eAED=0.42;_QI=159|1|1|1|1|1|2|273|329
Chr4 exon 34867720 34868690 34867720 ID=MsG0480020139.01.T01:exon:15437;Parent=MsG0480020139.01.T01
Chr4 exon 34866298 34866748 34866298 ID=MsG0480020139.01.T01:exon:15436;Parent=MsG0480020139.01.T01
Chr4 five_prime_UTR 34866298 34866456 34866298 ID=MsG0480020139.01.T01:five_prime_utr;Parent=MsG0480020139.01.T01
Chr4 CDS 34866457 34866748 34866457 ID=MsG0480020139.01.T01:cds;Parent=MsG0480020139.01.T01
Chr4 CDS 34867720 34868417 34867720 ID=MsG0480020139.01.T01:cds;Parent=MsG0480020139.01.T01
Chr4 three_prime_UTR 34868418 34868690 34868418 ID=MsG0480020139.01.T01:three_prime_utr;Parent=MsG0480020139.01.T01
Gene Sequence

>MsG0480020139.01.T01

ATGTCCACCACAATCCCATCCCTCCTCACCTTCAAATCCAAACTCTCACTCCCCTTCCCACGCCACCACCAACAACAATTCCCAACAACCACCACAAAATCCCTACCTTTCAACCCTCAATTCACTCGTAACCTAATTGGGTTTTCCCCCAAACCATTCAGATTTGCGCCGGTGAAGCGTTTTGCCACAAATAACGATGAAGCTGAGAAAGAGCCTAGTACTATTCCGGGAAGGTTTAAGGATTATATTAAACCAGCTCCTGAATCTCCTTTCAAGTGGCCCATGTTTGTAGCACTGGCTTTTCTTATCTATGCTTGGAGAGCTGTTCTATTTGAACTGTCAAACTGGAAGAACGCTGCATTTGGAATCGTTCAATTCGTAGGATATGTCTTGAAATATGCTTTTGCATTAGTCTATCGATTCATAGGAAATCCGATCACTTTTACAATCGGATCCATTGAGGATTTGATTTACGGCGTTCAGGCTTTTTACTCTTGGATAATTACCAGTGCCCCTGTACCAGACCTGACCCTCGTTATCGTCCTGGCATCAGTTGTTTTAGCTGTTGCTGAAACTACTAATCCAAATTGTATCAGCGACCAACCTTATGTTTTGACTGTAACTGGTCTTATTGGCTATGCTGCTGTTAAAGGCATTATTTCTGAACCACTGTTCTGGACACTTCTGGTTGGGATCTATGGCTTTTCAAAATTTATCAAAAGGAGAGACGATGCTTCATCTGCAATGCCTGTTGCAGCAGTACTTGCTGCGGTTGGAGAGCCTTGGGTTAGATTTGTGGTGATTGTCTCATACACAGCCTTGGCTATTTATCAGCATTCAAAAATGATTTCAGAAGGGAAAGAAGTTGAAGATATTGAACCACATAGGAGGAAGCTTCCAGTTCCTCTTTTTCTTGCAGCTTTAGCAATTGGCTTACGAGTTGCTGCTAACTGGGCCGGGTATCGGCACTTGACATGGATGGTAGTATGA
Protein sequence

>MsG0480020139.01.T01

MSTTIPSLLTFKSKLSLPFPRHHQQQFPTTTTKSLPFNPQFTRNLIGFSPKPFRFAPVKRFATNNDEAEKEPSTIPGRFKDYIKPAPESPFKWPMFVALAFLIYAWRAVLFELSNWKNAAFGIVQFVGYVLKYAFALVYRFIGNPITFTIGSIEDLIYGVQAFYSWIITSAPVPDLTLVIVLASVVLAVAETTNPNCISDQPYVLTVTGLIGYAAVKGIISEPLFWTLLVGIYGFSKFIKRRDDASSAMPVAAVLAAVGEPWVRFVVIVSYTALAIYQHSKMISEGKEVEDIEPHRRKLPVPLFLAALAIGLRVAANWAGYRHLTWMVV*