AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020544.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020544.01.T01 MTR_1g093095 70.323 155 27 2 111 246 99 253 5.44e-79 241
MsG0480020544.01.T01 MTR_4g059870 35.821 134 71 5 121 242 61 191 8.51e-16 77.4
MsG0480020544.01.T01 MTR_2g093960 31.818 154 88 5 114 252 48 199 2.45e-15 75.9
MsG0480020544.01.T01 MTR_1g016010 33.824 136 75 5 119 242 62 194 1.11e-14 73.9
MsG0480020544.01.T01 MTR_8g017210 29.885 174 106 6 81 242 45 214 3.34e-14 72.8
MsG0480020544.01.T01 MTR_2g099990 31.646 158 92 6 97 242 45 198 7.04e-14 71.6
MsG0480020544.01.T01 MTR_1g094115 35.115 131 69 6 124 242 56 182 1.28e-13 70.9
MsG0480020544.01.T01 MTR_1g094115 33.333 141 78 6 114 242 63 199 1.46e-13 70.9
MsG0480020544.01.T01 MTR_1g112270 33.333 141 78 6 114 242 71 207 1.93e-13 70.5
MsG0480020544.01.T01 MTR_1g112270 33.333 141 78 6 114 242 60 196 2.37e-13 70.1
MsG0480020544.01.T01 MTR_2g099990 34.884 129 68 6 126 242 5 129 7.70e-13 68.2
MsG0480020544.01.T01 MTR_2g090745 34.043 141 77 7 114 242 23 159 1.08e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020544.01.T01 AT1G08290 48.788 330 92 8 9 266 12 336 3.97e-94 281
MsG0480020544.01.T01 AT3G57670 69.143 175 35 3 111 269 201 372 5.01e-85 259
MsG0480020544.01.T01 AT1G51220 47.887 284 100 6 11 246 30 313 1.60e-84 256
MsG0480020544.01.T01 AT3G20880 66.480 179 40 2 109 271 238 412 2.24e-84 259
MsG0480020544.01.T01 AT1G13290 63.542 192 44 5 73 246 55 238 5.80e-81 246
MsG0480020544.01.T01 AT1G34790 56.422 218 69 4 74 268 85 299 1.37e-77 238
MsG0480020544.01.T01 AT1G34370 33.333 174 83 7 121 269 28 193 1.86e-17 80.9
MsG0480020544.01.T01 AT1G34370 30.734 218 110 8 85 269 194 403 2.32e-17 82.0
MsG0480020544.01.T01 AT1G34370 30.734 218 110 8 85 269 194 403 2.32e-17 82.0
MsG0480020544.01.T01 AT5G22890 45.455 88 45 3 158 242 268 355 2.21e-16 78.6
MsG0480020544.01.T01 AT5G03150 34.815 135 71 6 121 242 76 206 1.13e-15 77.0
MsG0480020544.01.T01 AT5G66730 34.559 136 73 5 119 242 53 184 6.90e-15 74.7
MsG0480020544.01.T01 AT5G60470 34.328 134 73 4 121 242 58 188 1.33e-14 73.6
MsG0480020544.01.T01 AT1G55110 33.577 137 74 6 119 242 84 216 1.37e-14 73.6
MsG0480020544.01.T01 AT1G55110 33.577 137 74 6 119 242 84 216 1.37e-14 73.6
MsG0480020544.01.T01 AT1G55110 33.577 137 74 6 119 242 84 216 1.37e-14 73.6
MsG0480020544.01.T01 AT5G60470 34.328 134 73 4 121 242 63 193 1.95e-14 73.2
MsG0480020544.01.T01 AT5G60470 34.328 134 73 4 121 242 67 197 2.04e-14 73.2
MsG0480020544.01.T01 AT3G45260 34.328 134 73 5 121 242 62 192 5.12e-14 72.0
MsG0480020544.01.T01 AT3G45260 34.328 134 73 5 121 242 62 192 5.12e-14 72.0
MsG0480020544.01.T01 AT1G03840 31.210 157 94 5 97 242 40 193 9.80e-14 71.2
MsG0480020544.01.T01 AT1G14580 31.210 157 92 6 98 242 53 205 1.08e-13 71.2
MsG0480020544.01.T01 AT1G14580 31.210 157 92 6 98 242 53 205 1.08e-13 71.2
MsG0480020544.01.T01 AT1G14580 31.210 157 92 6 98 242 67 219 1.11e-13 70.9
MsG0480020544.01.T01 AT5G44160 29.545 176 106 7 81 242 18 189 1.22e-13 70.9
MsG0480020544.01.T01 AT2G02080 29.936 157 94 6 98 242 54 206 1.53e-13 70.9
MsG0480020544.01.T01 AT2G02080 29.936 157 94 6 98 242 54 206 1.53e-13 70.9
MsG0480020544.01.T01 AT2G02080 29.936 157 94 6 98 242 54 206 1.53e-13 70.9
MsG0480020544.01.T01 AT2G02080 29.936 157 94 6 98 242 54 206 1.53e-13 70.9
MsG0480020544.01.T01 AT1G03840 32.857 140 80 5 114 242 55 191 1.56e-13 70.5
MsG0480020544.01.T01 AT2G02070 30.573 157 93 5 98 242 52 204 1.75e-13 70.5
MsG0480020544.01.T01 AT2G02070 30.573 157 93 5 98 242 52 204 1.75e-13 70.5
MsG0480020544.01.T01 AT5G44160 33.083 133 75 5 121 242 21 150 2.36e-13 70.1
MsG0480020544.01.T01 AT2G02080 34.109 129 69 6 126 242 5 129 3.82e-13 69.3
MsG0480020544.01.T01 AT2G02080 34.109 129 69 6 126 242 5 129 3.82e-13 69.3
MsG0480020544.01.T01 AT3G13810 31.915 141 81 5 114 242 87 224 4.06e-13 69.3
MsG0480020544.01.T01 AT3G13810 31.915 141 81 5 114 242 73 210 4.34e-13 69.3
MsG0480020544.01.T01 AT3G13810 29.936 157 95 5 98 242 65 218 4.63e-13 69.3
MsG0480020544.01.T01 AT3G13810 29.936 157 95 5 98 242 70 223 4.84e-13 69.3
MsG0480020544.01.T01 AT3G13810 29.936 157 95 5 98 242 70 223 4.84e-13 69.3
MsG0480020544.01.T01 AT3G13810 29.936 157 95 5 98 242 51 204 5.75e-13 68.9
MsG0480020544.01.T01 AT3G50700 34.043 141 77 6 114 242 50 186 7.43e-13 68.6
MsG0480020544.01.T01 AT4G02670 32.624 141 80 5 114 242 71 208 7.59e-13 68.6
MsG0480020544.01.T01 AT4G02670 32.624 141 80 5 114 242 69 206 8.36e-13 68.2

Find 47 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAGATCATATTAGGTCTTT+TGG 0.190747 4:+43195048 MsG0480020544.01.T01:CDS
GAAAACTTTGCACTCAAAAT+AGG 0.212077 4:-43192197 None:intergenic
AGAAAACCTTCCAGAGGTTT+TGG 0.230422 4:+43192375 MsG0480020544.01.T01:CDS
CCATGCTACTGTTGTGTTCA+AGG 0.271424 4:+43194808 MsG0480020544.01.T01:CDS
GATCTCTCAAAGATCATATT+AGG 0.336377 4:+43195040 MsG0480020544.01.T01:CDS
GAGACTGTGGCTTTGCATAT+TGG 0.341519 4:+43192272 MsG0480020544.01.T01:CDS
CATGTTGTTGTACCTATTGA+AGG 0.355291 4:-43192462 None:intergenic
AACTCAAAACCCTCAATTTG+TGG 0.388963 4:-43192242 None:intergenic
TCTTTCAAAGGCTTAGCTCT+TGG 0.403693 4:-43194853 None:intergenic
TATGAACTAGAGGAATTAGA+TGG 0.411662 4:-43192113 None:intergenic
TGTGTGCCTCTTAGTGAGTC+TGG 0.423624 4:-43194763 None:intergenic
AAAAGAATTGTGGCAAGTTA+TGG 0.431308 4:+43194983 MsG0480020544.01.T01:CDS
TATGCAACAAAACCTTCAAT+AGG 0.433146 4:+43192450 MsG0480020544.01.T01:CDS
ACTCCTGCACAGATCCTTGT+TGG 0.433190 4:+43192404 MsG0480020544.01.T01:CDS
GGTAAAGAATATGGCAATGA+TGG 0.440587 4:+43192049 None:intergenic
GATTGATGATCAAGCTTTCG+AGG 0.449639 4:+43195095 MsG0480020544.01.T01:CDS
ACGTCAAGTGCCACAAATTG+AGG 0.451497 4:+43192232 MsG0480020544.01.T01:CDS
CATTTGCTGTGAAAGGTGAT+TGG 0.459235 4:+43194950 MsG0480020544.01.T01:CDS
CCATTTATGTGTAGAAAATG+TGG 0.488125 4:+43194922 MsG0480020544.01.T01:CDS
GGTAGCCTTAGCATTGCAGC+TGG 0.491825 4:-43194787 None:intergenic
AAGTTATGGTATTGCACTTG+TGG 0.518903 4:+43194997 MsG0480020544.01.T01:CDS
TTTCTTCTTCTTCTTTAACA+TGG 0.521689 4:-43192343 None:intergenic
CGTCAAGTGCCACAAATTGA+GGG 0.531074 4:+43192233 MsG0480020544.01.T01:CDS
CACAACCAGCTGCAATGCTA+AGG 0.533499 4:+43194782 MsG0480020544.01.T01:CDS
AGAGTTCTAAAATCTTTCAA+AGG 0.533941 4:-43194865 None:intergenic
GAGTTGGTATCCAAAACCTC+TGG 0.540847 4:-43192385 None:intergenic
TCAAGCTTTCGAGGAGGAGA+AGG 0.557211 4:+43195104 MsG0480020544.01.T01:CDS
ACAAGGATCTGTGCAGGAGT+TGG 0.561642 4:-43192401 None:intergenic
GGTCCAACAAGGATCTGTGC+AGG 0.564499 4:-43192407 None:intergenic
AGGACAATAATTTCATTGAG+TGG 0.566958 4:+43192084 MsG0480020544.01.T01:CDS
AAAGGACCAGACTCACTAAG+AGG 0.575770 4:+43194757 MsG0480020544.01.T01:CDS
TTGCTGTGAAAGGTGATTGG+AGG 0.589362 4:+43194953 MsG0480020544.01.T01:CDS
TGAGTGGTGGAAGAAGAAGA+TGG 0.593836 4:-43192152 None:intergenic
CTTCTAAGAAAACCTTCCAG+AGG 0.598395 4:+43192369 MsG0480020544.01.T01:CDS
CATTTATGTGTAGAAAATGT+GGG 0.610048 4:+43194923 MsG0480020544.01.T01:CDS
ACTCACTACAAAAGAAAACA+TGG 0.615968 4:+43194892 MsG0480020544.01.T01:CDS
AAAGCTTGATCATCAATCGA+AGG 0.617239 4:-43195090 None:intergenic
AGGACTCATGAAAAGAATTG+TGG 0.623632 4:+43194973 MsG0480020544.01.T01:CDS
GGGAAAACATTTGCTGTGAA+AGG 0.623807 4:+43194943 MsG0480020544.01.T01:CDS
TGAAGTTAGATATGAACTAG+AGG 0.628204 4:-43192123 None:intergenic
TGGTATCCAAAACCTCTGGA+AGG 0.630382 4:-43192381 None:intergenic
TGAGTTCAAAGAAGAGACTG+TGG 0.637308 4:+43192259 MsG0480020544.01.T01:CDS
ATGGAGCAAGAAAATTGCAT+AGG 0.642845 4:-43192428 None:intergenic
AAAATTGCATAGGTCCAACA+AGG 0.669488 4:-43192418 None:intergenic
CCTTGAACACAACAGTAGCA+TGG 0.671135 4:-43194808 None:intergenic
AATGATGGAGAGTGATGTAG+AGG 0.688074 4:+43192064 MsG0480020544.01.T01:CDS
TGATGATCAAGCTTTCGAGG+AGG 0.694755 4:+43195098 MsG0480020544.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATTTATTAATTGAGT+TGG - Chr4:43194696-43194715 None:intergenic 10.0%
!! AATTAATTAGTATAATCAAA+AGG - Chr4:43194185-43194204 None:intergenic 10.0%
!! ATAACAAAAAAAAATATAGA+TGG + Chr4:43193076-43193095 MsG0480020544.01.T01:intron 10.0%
!! ATATAAATATAAATAAATGG+AGG + Chr4:43194068-43194087 MsG0480020544.01.T01:intron 10.0%
!! TAACAAAAAAAAATATAGAT+GGG + Chr4:43193077-43193096 MsG0480020544.01.T01:intron 10.0%
!! TATTGAATATAAAAACTATA+AGG + Chr4:43192758-43192777 MsG0480020544.01.T01:intron 10.0%
!!! AACTGATTTTTTTAAATAAA+AGG - Chr4:43192930-43192949 None:intergenic 10.0%
!!! ATGATAATTTATCTTTTTTT+AGG - Chr4:43194457-43194476 None:intergenic 10.0%
!!! GTTTCTTTTATATTTTATTT+TGG - Chr4:43194603-43194622 None:intergenic 10.0%
!! AAATAGCCTATAAAAAAAAA+AGG - Chr4:43193527-43193546 None:intergenic 15.0%
!! TGAAAGATTAACAAAATTAT+TGG + Chr4:43194316-43194335 MsG0480020544.01.T01:intron 15.0%
!!! AAGGCAAATTTTAATTATAA+GGG + Chr4:43192867-43192886 MsG0480020544.01.T01:intron 15.0%
!!! AATTTGAAGTAAACAAATTA+AGG - Chr4:43193391-43193410 None:intergenic 15.0%
!!! ATTTGAAGTAAACAAATTAA+GGG - Chr4:43193390-43193409 None:intergenic 15.0%
!!! ATTTTAATTATAAGGGATTT+TGG + Chr4:43192874-43192893 MsG0480020544.01.T01:intron 15.0%
!!! TATAGTTTTCCATTTTTTTA+GGG - Chr4:43192601-43192620 None:intergenic 15.0%
!!! TATTCTCCTTTTTTTTTTAT+AGG + Chr4:43193518-43193537 MsG0480020544.01.T01:intron 15.0%
!!! TCAAAAAGTTAATTTTTCAT+CGG + Chr4:43193692-43193711 MsG0480020544.01.T01:intron 15.0%
!!! TGGAAACATTTTTTTTTTTT+TGG - Chr4:43193466-43193485 None:intergenic 15.0%
!!! TTATAGTTTTCCATTTTTTT+AGG - Chr4:43192602-43192621 None:intergenic 15.0%
!! AAAATTATTGGCAACAATTA+TGG + Chr4:43194328-43194347 MsG0480020544.01.T01:intron 20.0%
!! AAGAAAACAAAACAAAACTT+TGG + Chr4:43194154-43194173 MsG0480020544.01.T01:intron 20.0%
!! CAGTTACTTATAAAGAAAAA+TGG + Chr4:43192567-43192586 MsG0480020544.01.T01:intron 20.0%
!!! AATTAACTTTTTGAAAGTGA+AGG - Chr4:43193686-43193705 None:intergenic 20.0%
!!! ACGTTTTTAATACTTAGTAA+TGG - Chr4:43193933-43193952 None:intergenic 20.0%
!!! ATAGTTTTCCATTTTTTTAG+GGG - Chr4:43192600-43192619 None:intergenic 20.0%
!!! ATTAACTTTTTGAAAGTGAA+GGG - Chr4:43193685-43193704 None:intergenic 20.0%
!!! CAAGGCAAATTTTAATTATA+AGG + Chr4:43192866-43192885 MsG0480020544.01.T01:intron 20.0%
!!! TATGGTCTAATTTAAAGTAA+GGG + Chr4:43194408-43194427 MsG0480020544.01.T01:intron 20.0%
!!! TGAAGATCAATTTTATTTCT+TGG + Chr4:43193959-43193978 MsG0480020544.01.T01:intron 20.0%
!!! TTATGGTCTAATTTAAAGTA+AGG + Chr4:43194407-43194426 MsG0480020544.01.T01:intron 20.0%
!!! TTGTTTTATTTCAACATTCA+TGG + Chr4:43194242-43194261 MsG0480020544.01.T01:intron 20.0%
! AATCAAAGATGACATGAATT+AGG + Chr4:43194564-43194583 MsG0480020544.01.T01:intron 25.0%
! ACAAAACAGACAATATTCTA+GGG - Chr4:43193809-43193828 None:intergenic 25.0%
! ATAAATAAATGGAGGACATT+GGG + Chr4:43194076-43194095 MsG0480020544.01.T01:intron 25.0%
! ATTCGTAAGTAAATTTAGTG+AGG - Chr4:43192681-43192700 None:intergenic 25.0%
! CAAAAAAAAATATAGATGGG+AGG + Chr4:43193080-43193099 MsG0480020544.01.T01:intron 25.0%
! CATTTATGTGTAGAAAATGT+GGG + Chr4:43194923-43194942 MsG0480020544.01.T01:CDS 25.0%
! TACAACAATTTCTTCTCTTA+AGG + Chr4:43194503-43194522 MsG0480020544.01.T01:intron 25.0%
! TATAAATAAATGGAGGACAT+TGG + Chr4:43194075-43194094 MsG0480020544.01.T01:intron 25.0%
! TTCGTAAGTAAATTTAGTGA+GGG - Chr4:43192680-43192699 None:intergenic 25.0%
! TTTCTTCTCTTAAGGTTATA+TGG + Chr4:43194511-43194530 MsG0480020544.01.T01:intron 25.0%
! TTTCTTCTTCTTCTTTAACA+TGG - Chr4:43192346-43192365 None:intergenic 25.0%
! TTTGTTAATCTTTCACTGAT+GGG - Chr4:43194311-43194330 None:intergenic 25.0%
!! AAAGATCATATTAGGTCTTT+TGG + Chr4:43195048-43195067 MsG0480020544.01.T01:CDS 25.0%
!! ATAGTAATTTGTCAAAAGGA+GGG + Chr4:43194101-43194120 MsG0480020544.01.T01:intron 25.0%
!! GTTAATTTTCAACCCAAAAA+AGG - Chr4:43193786-43193805 None:intergenic 25.0%
!! TTGTATAGTTTCATTTTGAG+TGG - Chr4:43192171-43192190 None:intergenic 25.0%
!! TTTTGTTAATCTTTCACTGA+TGG - Chr4:43194312-43194331 None:intergenic 25.0%
!!! AGAGTTCTAAAATCTTTCAA+AGG - Chr4:43194868-43194887 None:intergenic 25.0%
!!! ATCATCAAGATATGTTTTAG+TGG - Chr4:43192309-43192328 None:intergenic 25.0%
!!! ATTTGTGTTAGACCTTTTTT+GGG + Chr4:43193771-43193790 MsG0480020544.01.T01:intron 25.0%
!!! TATTTGTGTTAGACCTTTTT+TGG + Chr4:43193770-43193789 MsG0480020544.01.T01:intron 25.0%
AAAAGAATTGTGGCAAGTTA+TGG + Chr4:43194983-43195002 MsG0480020544.01.T01:CDS 30.0%
AAAGGAGAATATCAAATTGC+TGG - Chr4:43193509-43193528 None:intergenic 30.0%
AAGACAAGAAGAGAAAAGTT+AGG - Chr4:43193599-43193618 None:intergenic 30.0%
ACAATTATGGTCATATAGCT+AGG + Chr4:43194341-43194360 MsG0480020544.01.T01:intron 30.0%
ACTCACTACAAAAGAAAACA+TGG + Chr4:43194892-43194911 MsG0480020544.01.T01:CDS 30.0%
AGACAAGAAGAGAAAAGTTA+GGG - Chr4:43193598-43193617 None:intergenic 30.0%
AGGACAATAATTTCATTGAG+TGG + Chr4:43192084-43192103 MsG0480020544.01.T01:CDS 30.0%
ATAGATTAGCACCTACATTT+AGG + Chr4:43193414-43193433 MsG0480020544.01.T01:intron 30.0%
ATAGCTAGGTCAAATAATTC+AGG + Chr4:43194355-43194374 MsG0480020544.01.T01:intron 30.0%
ATCGGTTATGATCATTTCAA+AGG + Chr4:43193710-43193729 MsG0480020544.01.T01:intron 30.0%
CAAAACAGACAATATTCTAG+GGG - Chr4:43193808-43193827 None:intergenic 30.0%
CACAAAACAGACAATATTCT+AGG - Chr4:43193810-43193829 None:intergenic 30.0%
CCATTTATGTGTAGAAAATG+TGG + Chr4:43194922-43194941 MsG0480020544.01.T01:CDS 30.0%
CTTTGTGTTTGTCAAATTCA+TGG - Chr4:43193486-43193505 None:intergenic 30.0%
GAAAAACTCCCCTAAAAAAA+TGG + Chr4:43192589-43192608 MsG0480020544.01.T01:intron 30.0%
GAAAACTTTGCACTCAAAAT+AGG - Chr4:43192200-43192219 None:intergenic 30.0%
GATCTCTCAAAGATCATATT+AGG + Chr4:43195040-43195059 MsG0480020544.01.T01:CDS 30.0%
TAAAAACTATAAGGTCTAGC+AGG + Chr4:43192767-43192786 MsG0480020544.01.T01:intron 30.0%
TAAATAAATGGAGGACATTG+GGG + Chr4:43194077-43194096 MsG0480020544.01.T01:intron 30.0%
TATGAACTAGAGGAATTAGA+TGG - Chr4:43192116-43192135 None:intergenic 30.0%
TATGCAACAAAACCTTCAAT+AGG + Chr4:43192450-43192469 MsG0480020544.01.T01:CDS 30.0%
TCGGTTATGATCATTTCAAA+GGG + Chr4:43193711-43193730 MsG0480020544.01.T01:intron 30.0%
TGAAGTTAGATATGAACTAG+AGG - Chr4:43192126-43192145 None:intergenic 30.0%
TTGTTAATCTTTCACTGATG+GGG - Chr4:43194310-43194329 None:intergenic 30.0%
TTTCCATTAGATGCATATGT+GGG + Chr4:43194714-43194733 MsG0480020544.01.T01:intron 30.0%
! ATATTGTCTGTTTTGTGTGT+TGG + Chr4:43193813-43193832 MsG0480020544.01.T01:intron 30.0%
! CATAGTAATTTGTCAAAAGG+AGG + Chr4:43194100-43194119 MsG0480020544.01.T01:intron 30.0%
! CCACATTTTCTACACATAAA+TGG - Chr4:43194925-43194944 None:intergenic 30.0%
! GTTTTCTTTTGTAGTGAGTT+TGG - Chr4:43194891-43194910 None:intergenic 30.0%
! TTTTCCATTAGATGCATATG+TGG + Chr4:43194713-43194732 MsG0480020544.01.T01:intron 30.0%
!! CATATTAGGTCTTTTGGAAA+AGG + Chr4:43195054-43195073 MsG0480020544.01.T01:CDS 30.0%
!! TATAGTTTCATTTTGAGTGG+TGG - Chr4:43192168-43192187 None:intergenic 30.0%
!! TTTGTGTGTTGGTTAGTTAA+GGG + Chr4:43193824-43193843 MsG0480020544.01.T01:intron 30.0%
!!! AAGTTCCTTCATTTTTTGAG+TGG + Chr4:43192843-43192862 MsG0480020544.01.T01:intron 30.0%
!!! ATTGAAGGTTTTGTTGCATA+TGG - Chr4:43192450-43192469 None:intergenic 30.0%
!!! TAAAAGGAGCATCAGTTTTT+AGG + Chr4:43193319-43193338 MsG0480020544.01.T01:intron 30.0%
!!! TTTTGTGTGTTGGTTAGTTA+AGG + Chr4:43193823-43193842 MsG0480020544.01.T01:intron 30.0%
AAAATTGCATAGGTCCAACA+AGG - Chr4:43192421-43192440 None:intergenic 35.0%
AAAGCTTGATCATCAATCGA+AGG - Chr4:43195093-43195112 None:intergenic 35.0%
AACTCAAAACCCTCAATTTG+TGG - Chr4:43192245-43192264 None:intergenic 35.0%
AAGAAGAGAAAAGTTAGGGA+AGG - Chr4:43193594-43193613 None:intergenic 35.0%
AAGTTATGGTATTGCACTTG+TGG + Chr4:43194997-43195016 MsG0480020544.01.T01:CDS 35.0%
AGGACTCATGAAAAGAATTG+TGG + Chr4:43194973-43194992 MsG0480020544.01.T01:CDS 35.0%
ATGGAGCAAGAAAATTGCAT+AGG - Chr4:43192431-43192450 None:intergenic 35.0%
CATGGATCTGAATTTAGGAA+AGG + Chr4:43194739-43194758 MsG0480020544.01.T01:intron 35.0%
CATGTTGTTGTACCTATTGA+AGG - Chr4:43192465-43192484 None:intergenic 35.0%
CTAAAAATAGAGCTAGCAAC+AGG + Chr4:43193875-43193894 MsG0480020544.01.T01:intron 35.0%
TATGGTGATCTCTCGTTATT+GGG - Chr4:43193128-43193147 None:intergenic 35.0%
TTATGGTGATCTCTCGTTAT+TGG - Chr4:43193129-43193148 None:intergenic 35.0%
TTCCATTAGATGCATATGTG+GGG + Chr4:43194715-43194734 MsG0480020544.01.T01:intron 35.0%
! GGGCATAGTAATTTGTCAAA+AGG + Chr4:43194097-43194116 MsG0480020544.01.T01:intron 35.0%
! TAATTTGTCAAAAGGAGGGT+AGG + Chr4:43194105-43194124 MsG0480020544.01.T01:intron 35.0%
!!! AGATATGTTTTAGTGGCATG+AGG - Chr4:43192302-43192321 None:intergenic 35.0%
AAAGTGAAGGGAATGCTAGA+GGG - Chr4:43193673-43193692 None:intergenic 40.0%
AGAGATAGATGGAGAGAAGT+CGG + Chr4:43193183-43193202 MsG0480020544.01.T01:intron 40.0%
AGCACCTACATTTAGGCATA+CGG + Chr4:43193421-43193440 MsG0480020544.01.T01:intron 40.0%
CAATAGGTACAACAACATGC+AGG + Chr4:43192466-43192485 MsG0480020544.01.T01:intron 40.0%
CCTTGCCACTCAAAAAATGA+AGG - Chr4:43192851-43192870 None:intergenic 40.0%
TAATCTTTCACTGATGGGGT+AGG - Chr4:43194306-43194325 None:intergenic 40.0%
TCAAACTGATAGGGCATACA+GGG - Chr4:43193271-43193290 None:intergenic 40.0%
TCCATTAGATGCATATGTGG+GGG + Chr4:43194716-43194735 MsG0480020544.01.T01:intron 40.0%
TGAGTTCAAAGAAGAGACTG+TGG + Chr4:43192259-43192278 MsG0480020544.01.T01:CDS 40.0%
TTCCAAGACGAGAACTTCAT+TGG + Chr4:43193361-43193380 MsG0480020544.01.T01:intron 40.0%
TTCTACTTAACTGCGAGAGA+GGG + Chr4:43194011-43194030 MsG0480020544.01.T01:intron 40.0%
TTCTCTCCATCTATCTCTCT+GGG - Chr4:43193181-43193200 None:intergenic 40.0%
TTGTCCGTATGCCTAAATGT+AGG - Chr4:43193428-43193447 None:intergenic 40.0%
TTTCTACTTAACTGCGAGAG+AGG + Chr4:43194010-43194029 MsG0480020544.01.T01:intron 40.0%
! AATGATGGAGAGTGATGTAG+AGG + Chr4:43192064-43192083 MsG0480020544.01.T01:CDS 40.0%
! ACTGATGCTCCTTTTATGAG+TGG - Chr4:43193315-43193334 None:intergenic 40.0%
! AGAAAACCTTCCAGAGGTTT+TGG + Chr4:43192375-43192394 MsG0480020544.01.T01:CDS 40.0%
! CTCGTTATTGGGACTCTTTT+GGG - Chr4:43193117-43193136 None:intergenic 40.0%
! CTGATGCTCCTTTTATGAGT+GGG - Chr4:43193314-43193333 None:intergenic 40.0%
! CTTCTAAGAAAACCTTCCAG+AGG + Chr4:43192369-43192388 MsG0480020544.01.T01:CDS 40.0%
! GATTGATGATCAAGCTTTCG+AGG + Chr4:43195095-43195114 MsG0480020544.01.T01:CDS 40.0%
! TCTCGTTATTGGGACTCTTT+TGG - Chr4:43193118-43193137 None:intergenic 40.0%
! TCTTTCAAAGGCTTAGCTCT+TGG - Chr4:43194856-43194875 None:intergenic 40.0%
! TTTAGAGGCGGTGTTTGTTA+TGG - Chr4:43193146-43193165 None:intergenic 40.0%
!! CATTTGCTGTGAAAGGTGAT+TGG + Chr4:43194950-43194969 MsG0480020544.01.T01:CDS 40.0%
!! CTCTGGAAGGTTTTCTTAGA+AGG - Chr4:43192371-43192390 None:intergenic 40.0%
!! GGGAAAACATTTGCTGTGAA+AGG + Chr4:43194943-43194962 MsG0480020544.01.T01:CDS 40.0%
!!! CCTTCATTTTTTGAGTGGCA+AGG + Chr4:43192848-43192867 MsG0480020544.01.T01:intron 40.0%
AAAGGACCAGACTCACTAAG+AGG + Chr4:43194757-43194776 MsG0480020544.01.T01:CDS 45.0%
ACGTCAAGTGCCACAAATTG+AGG + Chr4:43192232-43192251 MsG0480020544.01.T01:CDS 45.0%
ATAGATGGAGAGAAGTCGGA+TGG + Chr4:43193187-43193206 MsG0480020544.01.T01:intron 45.0%
ATCCCGTGCTCAAACTGATA+GGG - Chr4:43193280-43193299 None:intergenic 45.0%
ATGCCCTATCAGTTTGAGCA+CGG + Chr4:43193274-43193293 MsG0480020544.01.T01:intron 45.0%
CCATGCTACTGTTGTGTTCA+AGG + Chr4:43194808-43194827 MsG0480020544.01.T01:CDS 45.0%
CCTTGAACACAACAGTAGCA+TGG - Chr4:43194811-43194830 None:intergenic 45.0%
CGTCAAGTGCCACAAATTGA+GGG + Chr4:43192233-43192252 MsG0480020544.01.T01:CDS 45.0%
CTATAAGGTCTAGCAGGAGA+AGG + Chr4:43192773-43192792 MsG0480020544.01.T01:intron 45.0%
CTCAAACTGATAGGGCATAC+AGG - Chr4:43193272-43193291 None:intergenic 45.0%
CTTCTCTCCATCTATCTCTC+TGG - Chr4:43193182-43193201 None:intergenic 45.0%
GAAAGTGAAGGGAATGCTAG+AGG - Chr4:43193674-43193693 None:intergenic 45.0%
GTGTAGGACATGTAACATCG+AGG + Chr4:43194127-43194146 MsG0480020544.01.T01:intron 45.0%
TATCAGTTTGAGCACGGGAT+AGG + Chr4:43193280-43193299 MsG0480020544.01.T01:intron 45.0%
TATCCCGTGCTCAAACTGAT+AGG - Chr4:43193281-43193300 None:intergenic 45.0%
TCGAGCTAGCTATAGCTAGA+TGG + Chr4:43193637-43193656 MsG0480020544.01.T01:intron 45.0%
TCGTCTTGGAAACGACTCAT+TGG - Chr4:43193352-43193371 None:intergenic 45.0%
TGATGATCAAGCTTTCGAGG+AGG + Chr4:43195098-43195117 MsG0480020544.01.T01:CDS 45.0%
TGCCAATGAAGTTCTCGTCT+TGG - Chr4:43193366-43193385 None:intergenic 45.0%
TGGTATCCAAAACCTCTGGA+AGG - Chr4:43192384-43192403 None:intergenic 45.0%
TTGCACCTCCCACTCATAAA+AGG + Chr4:43193303-43193322 MsG0480020544.01.T01:intron 45.0%
TTGCTGTGAAAGGTGATTGG+AGG + Chr4:43194953-43194972 MsG0480020544.01.T01:CDS 45.0%
! ATGCTCCTTTTATGAGTGGG+AGG - Chr4:43193311-43193330 None:intergenic 45.0%
! GAGACTGTGGCTTTGCATAT+TGG + Chr4:43192272-43192291 MsG0480020544.01.T01:CDS 45.0%
! TGAGTGGTGGAAGAAGAAGA+TGG - Chr4:43192155-43192174 None:intergenic 45.0%
!! GAGTTGGTATCCAAAACCTC+TGG - Chr4:43192388-43192407 None:intergenic 45.0%
!!! TGGGTTGGTGATTTTAGAGG+CGG - Chr4:43193158-43193177 None:intergenic 45.0%
!! ACTATATAAATATAAATAAA+TGG + Chr4:43194065-43194084 MsG0480020544.01.T01:intron 5.0%
!!! GATTATTATTTATTTTTTTA+TGG + Chr4:43194390-43194409 MsG0480020544.01.T01:intron 5.0%
!!! TATATTTTTTTTTGTTATAT+CGG - Chr4:43193074-43193093 None:intergenic 5.0%
!!! TTTCTTTTATATTTTATTTT+GGG - Chr4:43194602-43194621 None:intergenic 5.0%
AACCCACCCAGAGAGATAGA+TGG + Chr4:43193172-43193191 MsG0480020544.01.T01:intron 50.0%
ACAAGGATCTGTGCAGGAGT+TGG - Chr4:43192404-43192423 None:intergenic 50.0%
ACTCCTGCACAGATCCTTGT+TGG + Chr4:43192404-43192423 MsG0480020544.01.T01:CDS 50.0%
ATCTATCTCTCTGGGTGGGT+TGG - Chr4:43193173-43193192 None:intergenic 50.0%
ATGGAGAGAAGTCGGATGGA+GGG + Chr4:43193191-43193210 MsG0480020544.01.T01:intron 50.0%
CACAACCAGCTGCAATGCTA+AGG + Chr4:43194782-43194801 MsG0480020544.01.T01:CDS 50.0%
CTCCATCTATCTCTCTGGGT+GGG - Chr4:43193177-43193196 None:intergenic 50.0%
GAATGCTAGAGGGACACAAG+AGG - Chr4:43193663-43193682 None:intergenic 50.0%
GATAGGGCATACAGGGTTGT+TGG - Chr4:43193264-43193283 None:intergenic 50.0%
GCATACAGGGTTGTTGGAGA+TGG - Chr4:43193258-43193277 None:intergenic 50.0%
GCCCCCACATATGCATCTAA+TGG - Chr4:43194720-43194739 None:intergenic 50.0%
GTCAAAAGGAGGGTAGGTGT+AGG + Chr4:43194111-43194130 MsG0480020544.01.T01:intron 50.0%
TAGATGCATATGTGGGGGCA+TGG + Chr4:43194721-43194740 MsG0480020544.01.T01:intron 50.0%
TCAAGCTTTCGAGGAGGAGA+AGG + Chr4:43195104-43195123 MsG0480020544.01.T01:CDS 50.0%
TCTCCATCTATCTCTCTGGG+TGG - Chr4:43193178-43193197 None:intergenic 50.0%
TGCCCTATCAGTTTGAGCAC+GGG + Chr4:43193275-43193294 MsG0480020544.01.T01:intron 50.0%
TGTGTGCCTCTTAGTGAGTC+TGG - Chr4:43194766-43194785 None:intergenic 50.0%
! GGGGGCATGGATCTGAATTT+AGG + Chr4:43194734-43194753 MsG0480020544.01.T01:intron 50.0%
!!! GGGTGGGTTGGTGATTTTAG+AGG - Chr4:43193161-43193180 None:intergenic 50.0%
ATGGATCACGATCGCCTGGT+TGG - Chr4:43193239-43193258 None:intergenic 55.0%
GAGCAGCCATAAGACCAACC+AGG + Chr4:43193222-43193241 MsG0480020544.01.T01:intron 55.0%
GATGGAGAGAAGTCGGATGG+AGG + Chr4:43193190-43193209 MsG0480020544.01.T01:intron 55.0%
GGTCCAACAAGGATCTGTGC+AGG - Chr4:43192410-43192429 None:intergenic 55.0%
TGGAGAGAAGTCGGATGGAG+GGG + Chr4:43193192-43193211 MsG0480020544.01.T01:intron 55.0%
! GGTAGCCTTAGCATTGCAGC+TGG - Chr4:43194790-43194809 None:intergenic 55.0%
!! CGATCGCCTGGTTGGTCTTA+TGG - Chr4:43193231-43193250 None:intergenic 55.0%
GGAGATGGATCACGATCGCC+TGG - Chr4:43193243-43193262 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 43192059 43195170 43192059 ID=MsG0480020544.01;Name=MsG0480020544.01
Chr4 mRNA 43192059 43195170 43192059 ID=MsG0480020544.01.T01;Parent=MsG0480020544.01;Name=MsG0480020544.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|275
Chr4 exon 43192059 43192471 43192059 ID=MsG0480020544.01.T01:exon:15603;Parent=MsG0480020544.01.T01
Chr4 exon 43194756 43195170 43194756 ID=MsG0480020544.01.T01:exon:15604;Parent=MsG0480020544.01.T01
Chr4 CDS 43192059 43192471 43192059 ID=MsG0480020544.01.T01:cds;Parent=MsG0480020544.01.T01
Chr4 CDS 43194756 43195170 43194756 ID=MsG0480020544.01.T01:cds;Parent=MsG0480020544.01.T01
Gene Sequence

>MsG0480020544.01.T01

ATGGCAATGATGGAGAGTGATGTAGAGGACAATAATTTCATTGAGTGGCTCAAACCATCTAATTCCTCTAGTTCATATCTAACTTCATCATCACCATCTTCTTCTTCCACCACTCAAAATGAAACTATACAATTCTTTCCTATTTTGAGTGCAAAGTTTTCAAAAGATGAAGAACGTCAAGTGCCACAAATTGAGGGTTTTGAGTTCAAAGAAGAGACTGTGGCTTTGCATATTGGATTACCTCATGCCACTAAAACATATCTTGATGATGAGAAGAAGTTTTTCCATGTTAAAGAAGAAGAAGAAAAGCCTTCTAAGAAAACCTTCCAGAGGTTTTGGATACCAACTCCTGCACAGATCCTTGTTGGACCTATGCAATTTTCTTGCTCCATATGCAACAAAACCTTCAATAGGAAAGGACCAGACTCACTAAGAGGCACACAACCAGCTGCAATGCTAAGGCTACCATGCTACTGTTGTGTTCAAGGTTGCAAGAACAACATCAATCATCCAAGAGCTAAGCCTTTGAAAGATTTTAGAACTCTCCAAACTCACTACAAAAGAAAACATGGAACAAAACCATTTATGTGTAGAAAATGTGGGAAAACATTTGCTGTGAAAGGTGATTGGAGGACTCATGAAAAGAATTGTGGCAAGTTATGGTATTGCACTTGTGGTTCAGATTTTAAACACAAAAGATCTCTCAAAGATCATATTAGGTCTTTTGGAAAAGGTCATCGTCGTCTTCCTTCGATTGATGATCAAGCTTTCGAGGAGGAGAAGGAATGTGTTACTGTCTCTGAAGAAGATGAATTTGCTCGTACATGA
Protein sequence

>MsG0480020544.01.T01

MAMMESDVEDNNFIEWLKPSNSSSSYLTSSSPSSSSTTQNETIQFFPILSAKFSKDEERQVPQIEGFEFKEETVALHIGLPHATKTYLDDEKKFFHVKEEEEKPSKKTFQRFWIPTPAQILVGPMQFSCSICNKTFNRKGPDSLRGTQPAAMLRLPCYCCVQGCKNNINHPRAKPLKDFRTLQTHYKRKHGTKPFMCRKCGKTFAVKGDWRTHEKNCGKLWYCTCGSDFKHKRSLKDHIRSFGKGHRRLPSIDDQAFEEEKECVTVSEEDEFART*