AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020547.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020547.01.T01 MTR_4g053440 94.118 170 6 2 1 166 1 170 7.93e-115 323
MsG0480020547.01.T01 MTR_2g086190 57.229 166 55 3 1 166 1 150 8.97e-59 180
MsG0480020547.01.T01 MTR_7g104320 51.136 176 68 4 1 166 1 168 2.58e-57 177
MsG0480020547.01.T01 MTR_1g060380 50.292 171 65 4 1 166 1 156 4.40e-47 150
MsG0480020547.01.T01 MTR_2g098160 41.520 171 83 5 7 166 8 172 1.42e-40 134
MsG0480020547.01.T01 MTR_0249s0070 42.012 169 89 5 3 166 5 169 3.95e-39 131
MsG0480020547.01.T01 MTR_0249s0070 42.012 169 89 5 3 166 13 177 4.98e-39 131
MsG0480020547.01.T01 MTR_7g092400 39.011 182 79 5 3 166 93 260 1.37e-37 129
MsG0480020547.01.T01 MTR_7g114920 40.789 152 63 5 13 163 5 130 2.54e-31 110
MsG0480020547.01.T01 MTR_4g065570 37.931 145 75 3 28 166 2 137 9.14e-31 108
MsG0480020547.01.T01 MTR_1g100773 39.073 151 74 4 13 163 5 137 5.99e-30 107
MsG0480020547.01.T01 MTR_3g028010 33.766 154 84 2 13 166 28 163 1.97e-24 93.2
MsG0480020547.01.T01 MTR_8g036980 56.452 62 27 0 105 166 90 151 1.74e-22 88.2
MsG0480020547.01.T01 MTR_2g054650 38.158 152 60 7 13 163 6 124 1.03e-19 80.1
MsG0480020547.01.T01 MTR_3g025570 54.545 44 20 0 101 144 4 47 6.71e-12 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020547.01.T01 AT2G27580 56.627 166 69 2 1 166 1 163 1.01e-55 173
MsG0480020547.01.T01 AT2G27580 56.627 166 69 2 1 166 1 163 1.01e-55 173
MsG0480020547.01.T01 AT2G36320 49.405 168 76 2 1 166 1 161 9.90e-52 162
MsG0480020547.01.T01 AT1G12440 45.806 155 81 1 12 166 17 168 1.55e-45 147
MsG0480020547.01.T01 AT1G12440 45.806 155 81 1 12 166 17 168 1.55e-45 147
MsG0480020547.01.T01 AT1G12440 45.806 155 81 1 12 166 17 168 1.55e-45 147
MsG0480020547.01.T01 AT3G52800 48.276 174 78 3 1 166 1 170 3.17e-43 141
MsG0480020547.01.T01 AT3G52800 48.276 174 78 3 1 166 1 170 3.17e-43 141
MsG0480020547.01.T01 AT4G12040 43.529 170 70 4 12 166 17 175 5.34e-42 138
MsG0480020547.01.T01 AT4G12040 43.529 170 70 4 12 166 17 175 5.34e-42 138
MsG0480020547.01.T01 AT4G22820 44.099 161 80 4 12 166 20 176 1.02e-41 138
MsG0480020547.01.T01 AT4G22820 44.099 161 80 4 12 166 20 176 1.02e-41 138
MsG0480020547.01.T01 AT1G51200 44.099 161 81 4 12 166 16 173 1.20e-37 127
MsG0480020547.01.T01 AT1G51200 44.099 161 81 4 12 166 16 173 1.20e-37 127
MsG0480020547.01.T01 AT1G51200 44.099 161 81 4 12 166 16 173 1.20e-37 127
MsG0480020547.01.T01 AT1G51200 44.099 161 81 4 12 166 16 173 1.20e-37 127
MsG0480020547.01.T01 AT3G12630 34.211 152 74 4 19 166 31 160 1.66e-24 93.6
MsG0480020547.01.T01 AT4G25380 34.641 153 68 6 14 166 10 130 4.19e-23 89.0
MsG0480020547.01.T01 AT4G14225 31.613 155 69 2 13 166 7 125 2.91e-20 81.6

Find 55 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACGTTCTTCATCTGCAAAAT+TGG 0.253435 4:-43225517 MsG0480020547.01.T01:CDS
CTGCATGTCCCACACCTATT+TGG 0.276997 4:+43225333 None:intergenic
GCTAACAACTGCGGTTTCTT+TGG 0.331458 4:-43225600 MsG0480020547.01.T01:CDS
CACCTATTTGGCTGGACCTT+CGG 0.347652 4:+43225345 None:intergenic
TATCCGGAGCAGCACGGTTG+TGG 0.350405 4:-43225246 MsG0480020547.01.T01:CDS
CTGCAAATCACGGTAACATT+TGG 0.369389 4:+43225551 None:intergenic
AGCTTCTCTCCCTTCACCAC+TGG 0.384998 4:+43225177 None:intergenic
GTGGAGTTGGTAGCTGCTGC+TGG 0.406487 4:-43225387 MsG0480020547.01.T01:CDS
CACCGAAGGTCCAGCCAAAT+AGG 0.408383 4:-43225347 MsG0480020547.01.T01:CDS
TGGGACATGCAGACGGCGCG+TGG 0.435856 4:-43225322 MsG0480020547.01.T01:CDS
GTGTGGGACCCACAGGTATC+CGG 0.436288 4:-43225262 MsG0480020547.01.T01:CDS
ACTGTTGAGGAATCAACGTT+TGG 0.447132 4:+43225487 None:intergenic
GAGCAAAGATCTTGCATGGC+AGG 0.449620 4:+43225573 None:intergenic
AACCCACAACCGTGCTGCTC+CGG 0.450007 4:+43225243 None:intergenic
GGTAGCTGCTGCTGGACCTT+CGG 0.453896 4:-43225379 MsG0480020547.01.T01:CDS
AGACGGCGCGTGGGGCTGAC+AGG 0.473736 4:-43225312 MsG0480020547.01.T01:CDS
ATGTCCCACACCTATTTGGC+TGG 0.481879 4:+43225337 None:intergenic
ATCCGGAGCAGCACGGTTGT+GGG 0.489485 4:-43225245 MsG0480020547.01.T01:CDS
CACAAAGACGTTGAGCAGCT+TGG 0.496369 4:+43225622 None:intergenic
TTACCGTGATTTGCAGATGA+AGG 0.514621 4:-43225544 MsG0480020547.01.T01:CDS
ACTACAACCGACGGTGGAGA+TGG 0.535288 4:+43225429 None:intergenic
CGTGCTGCTCCGGATACCTG+TGG 0.543879 4:+43225253 None:intergenic
GAAGGCGAATCCAGTGGTGA+AGG 0.546816 4:-43225187 MsG0480020547.01.T01:CDS
ATCTACACCGACGGTGGAGT+TGG 0.547793 4:-43225400 MsG0480020547.01.T01:CDS
TGACGTTGTGTGGGACCCAC+AGG 0.547943 4:-43225269 MsG0480020547.01.T01:CDS
CAGGATTTAAGTGTCGGTGC+GGG 0.548307 4:-43225293 MsG0480020547.01.T01:CDS
CGGTGCGGGTTGACGTTGTG+TGG 0.556113 4:-43225279 MsG0480020547.01.T01:CDS
TTCGGTGGTTCAGCCTCCGA+AGG 0.562954 4:+43225363 None:intergenic
CAAGAAGAATTAGACTGTTG+AGG 0.566912 4:+43225474 None:intergenic
GGCTGACAGGATTTAAGTGT+CGG 0.567077 4:-43225299 MsG0480020547.01.T01:CDS
CTATTTGGCTGGACCTTCGG+TGG 0.568326 4:+43225348 None:intergenic
AGCTGCTGCTGGACCTTCGG+AGG 0.570808 4:-43225376 MsG0480020547.01.T01:CDS
TTTGGAGCAAAGATCTTGCA+TGG 0.576644 4:+43225569 None:intergenic
GGGACATGCAGACGGCGCGT+GGG 0.582538 4:-43225321 MsG0480020547.01.T01:CDS
GGACATGCAGACGGCGCGTG+GGG 0.594994 4:-43225320 MsG0480020547.01.T01:CDS
GTGCTGCTCCGGATACCTGT+GGG 0.602557 4:+43225254 None:intergenic
GTTCCTTCATCTGCAAATCA+CGG 0.602813 4:+43225541 None:intergenic
AAGGTCCAGCCAAATAGGTG+TGG 0.606672 4:-43225342 MsG0480020547.01.T01:CDS
TATTCATCCATCTCCACCGT+CGG 0.610000 4:-43225436 MsG0480020547.01.T01:CDS
AAGGCGAATCCAGTGGTGAA+GGG 0.610190 4:-43225186 MsG0480020547.01.T01:CDS
AGGTCCAGCCAAATAGGTGT+GGG 0.616831 4:-43225341 MsG0480020547.01.T01:CDS
ACAGGATTTAAGTGTCGGTG+CGG 0.618927 4:-43225294 MsG0480020547.01.T01:CDS
TTCGGAGGCTGAACCACCGA+AGG 0.620244 4:-43225361 MsG0480020547.01.T01:CDS
TAATTCTTCTTGTCTAGACA+CGG 0.623144 4:-43225463 MsG0480020547.01.T01:CDS
GGTGCGGGTTGACGTTGTGT+GGG 0.623964 4:-43225278 MsG0480020547.01.T01:CDS
CGTCTTTGTGCTAACAACTG+CGG 0.629379 4:-43225609 MsG0480020547.01.T01:CDS
GCAGCTACCAACTCCACCGT+CGG 0.632431 4:+43225393 None:intergenic
ATAGGTGTGGGACATGCAGA+CGG 0.635820 4:-43225329 MsG0480020547.01.T01:CDS
AGTTTCTTCATCTACACCGA+CGG 0.637393 4:-43225409 MsG0480020547.01.T01:CDS
TTCTTCATCTACACCGACGG+TGG 0.645268 4:-43225406 MsG0480020547.01.T01:CDS
GGGAAGGGAGGAGATCAAGA+AGG 0.649649 4:-43225205 MsG0480020547.01.T01:CDS
CACAGGTATCCGGAGCAGCA+CGG 0.653832 4:-43225252 MsG0480020547.01.T01:CDS
GATCAAGAAGGCGAATCCAG+TGG 0.675101 4:-43225193 MsG0480020547.01.T01:CDS
GATGAAGAAACTACAACCGA+CGG 0.679990 4:+43225420 None:intergenic
GAAGAAACTACAACCGACGG+TGG 0.716652 4:+43225423 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TAATTCTTCTTGTCTAGACA+CGG - Chr4:43225343-43225362 MsG0480020547.01.T01:CDS 30.0%
ACGTTCTTCATCTGCAAAAT+TGG - Chr4:43225289-43225308 MsG0480020547.01.T01:CDS 35.0%
CAAGAAGAATTAGACTGTTG+AGG + Chr4:43225335-43225354 None:intergenic 35.0%
!! GGTTGTGGGTTTGATTTTAA+GGG - Chr4:43225575-43225594 MsG0480020547.01.T01:CDS 35.0%
!! GTTGTGGGTTTGATTTTAAG+GGG - Chr4:43225576-43225595 MsG0480020547.01.T01:CDS 35.0%
ACTGTTGAGGAATCAACGTT+TGG + Chr4:43225322-43225341 None:intergenic 40.0%
AGTTTCTTCATCTACACCGA+CGG - Chr4:43225397-43225416 MsG0480020547.01.T01:CDS 40.0%
CTGCAAATCACGGTAACATT+TGG + Chr4:43225258-43225277 None:intergenic 40.0%
GATGAAGAAACTACAACCGA+CGG + Chr4:43225389-43225408 None:intergenic 40.0%
GTTCCTTCATCTGCAAATCA+CGG + Chr4:43225268-43225287 None:intergenic 40.0%
! TTACCGTGATTTGCAGATGA+AGG - Chr4:43225262-43225281 MsG0480020547.01.T01:CDS 40.0%
! TTTGGAGCAAAGATCTTGCA+TGG + Chr4:43225240-43225259 None:intergenic 40.0%
!! CGGTTGTGGGTTTGATTTTA+AGG - Chr4:43225574-43225593 MsG0480020547.01.T01:CDS 40.0%
!! GGGTTTGATTTTAAGGGGAT+GGG - Chr4:43225581-43225600 MsG0480020547.01.T01:CDS 40.0%
!! TGATTTTAAGGGGATGGGAA+GGG - Chr4:43225586-43225605 MsG0480020547.01.T01:CDS 40.0%
!! TGGGTTTGATTTTAAGGGGA+TGG - Chr4:43225580-43225599 MsG0480020547.01.T01:CDS 40.0%
!! TTGATTTTAAGGGGATGGGA+AGG - Chr4:43225585-43225604 MsG0480020547.01.T01:CDS 40.0%
CGTCTTTGTGCTAACAACTG+CGG - Chr4:43225197-43225216 MsG0480020547.01.T01:CDS 45.0%
GCTAACAACTGCGGTTTCTT+TGG - Chr4:43225206-43225225 MsG0480020547.01.T01:CDS 45.0%
GGCTGACAGGATTTAAGTGT+CGG - Chr4:43225507-43225526 MsG0480020547.01.T01:CDS 45.0%
TATTCATCCATCTCCACCGT+CGG - Chr4:43225370-43225389 MsG0480020547.01.T01:CDS 45.0%
!! ACAGGATTTAAGTGTCGGTG+CGG - Chr4:43225512-43225531 MsG0480020547.01.T01:CDS 45.0%
AAGGCGAATCCAGTGGTGAA+GGG - Chr4:43225620-43225639 MsG0480020547.01.T01:CDS 50.0%
AAGGTCCAGCCAAATAGGTG+TGG - Chr4:43225464-43225483 MsG0480020547.01.T01:CDS 50.0%
AGGTCCAGCCAAATAGGTGT+GGG - Chr4:43225465-43225484 MsG0480020547.01.T01:CDS 50.0%
ATAGGTGTGGGACATGCAGA+CGG - Chr4:43225477-43225496 MsG0480020547.01.T01:CDS 50.0%
ATGTCCCACACCTATTTGGC+TGG + Chr4:43225472-43225491 None:intergenic 50.0%
CACAAAGACGTTGAGCAGCT+TGG + Chr4:43225187-43225206 None:intergenic 50.0%
CACCTATTTGGCTGGACCTT+CGG + Chr4:43225464-43225483 None:intergenic 50.0%
CTGCATGTCCCACACCTATT+TGG + Chr4:43225476-43225495 None:intergenic 50.0%
GAAGAAACTACAACCGACGG+TGG + Chr4:43225386-43225405 None:intergenic 50.0%
GAGCAAAGATCTTGCATGGC+AGG + Chr4:43225236-43225255 None:intergenic 50.0%
GATCAAGAAGGCGAATCCAG+TGG - Chr4:43225613-43225632 MsG0480020547.01.T01:CDS 50.0%
TTCTTCATCTACACCGACGG+TGG - Chr4:43225400-43225419 MsG0480020547.01.T01:CDS 50.0%
!! CAGGATTTAAGTGTCGGTGC+GGG - Chr4:43225513-43225532 MsG0480020547.01.T01:CDS 50.0%
!! TTTTAAGGGGATGGGAAGGG+AGG - Chr4:43225589-43225608 MsG0480020547.01.T01:CDS 50.0%
ACTACAACCGACGGTGGAGA+TGG + Chr4:43225380-43225399 None:intergenic 55.0%
AGCTTCTCTCCCTTCACCAC+TGG + Chr4:43225632-43225651 None:intergenic 55.0%
ATCTACACCGACGGTGGAGT+TGG - Chr4:43225406-43225425 MsG0480020547.01.T01:CDS 55.0%
CACCGAAGGTCCAGCCAAAT+AGG - Chr4:43225459-43225478 MsG0480020547.01.T01:CDS 55.0%
CTATTTGGCTGGACCTTCGG+TGG + Chr4:43225461-43225480 None:intergenic 55.0%
GAAGGCGAATCCAGTGGTGA+AGG - Chr4:43225619-43225638 MsG0480020547.01.T01:CDS 55.0%
GGGAAGGGAGGAGATCAAGA+AGG - Chr4:43225601-43225620 MsG0480020547.01.T01:CDS 55.0%
ATCCGGAGCAGCACGGTTGT+GGG - Chr4:43225561-43225580 MsG0480020547.01.T01:CDS 60.0%
CACAGGTATCCGGAGCAGCA+CGG - Chr4:43225554-43225573 MsG0480020547.01.T01:CDS 60.0%
GCAGCTACCAACTCCACCGT+CGG + Chr4:43225416-43225435 None:intergenic 60.0%
GGTAGCTGCTGCTGGACCTT+CGG - Chr4:43225427-43225446 MsG0480020547.01.T01:CDS 60.0%
GTGTGGGACCCACAGGTATC+CGG - Chr4:43225544-43225563 MsG0480020547.01.T01:CDS 60.0%
TATCCGGAGCAGCACGGTTG+TGG - Chr4:43225560-43225579 MsG0480020547.01.T01:CDS 60.0%
TGACGTTGTGTGGGACCCAC+AGG - Chr4:43225537-43225556 MsG0480020547.01.T01:CDS 60.0%
TTCGGAGGCTGAACCACCGA+AGG - Chr4:43225445-43225464 MsG0480020547.01.T01:CDS 60.0%
TTCGGTGGTTCAGCCTCCGA+AGG + Chr4:43225446-43225465 None:intergenic 60.0%
! AACCCACAACCGTGCTGCTC+CGG + Chr4:43225566-43225585 None:intergenic 60.0%
! GGTGCGGGTTGACGTTGTGT+GGG - Chr4:43225528-43225547 MsG0480020547.01.T01:CDS 60.0%
! GTGCTGCTCCGGATACCTGT+GGG + Chr4:43225555-43225574 None:intergenic 60.0%
!! GTGGAGTTGGTAGCTGCTGC+TGG - Chr4:43225419-43225438 MsG0480020547.01.T01:CDS 60.0%
AGCTGCTGCTGGACCTTCGG+AGG - Chr4:43225430-43225449 MsG0480020547.01.T01:CDS 65.0%
! CGGTGCGGGTTGACGTTGTG+TGG - Chr4:43225527-43225546 MsG0480020547.01.T01:CDS 65.0%
! CGTGCTGCTCCGGATACCTG+TGG + Chr4:43225556-43225575 None:intergenic 65.0%
GGACATGCAGACGGCGCGTG+GGG - Chr4:43225486-43225505 MsG0480020547.01.T01:CDS 70.0%
GGGACATGCAGACGGCGCGT+GGG - Chr4:43225485-43225504 MsG0480020547.01.T01:CDS 70.0%
TGGGACATGCAGACGGCGCG+TGG - Chr4:43225484-43225503 MsG0480020547.01.T01:CDS 70.0%
AGACGGCGCGTGGGGCTGAC+AGG - Chr4:43225494-43225513 MsG0480020547.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 43225164 43225664 43225164 ID=MsG0480020547.01;Name=MsG0480020547.01
Chr4 mRNA 43225164 43225664 43225164 ID=MsG0480020547.01.T01;Parent=MsG0480020547.01;Name=MsG0480020547.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|166
Chr4 exon 43225164 43225664 43225164 ID=MsG0480020547.01.T01:exon:15645;Parent=MsG0480020547.01.T01
Chr4 CDS 43225164 43225664 43225164 ID=MsG0480020547.01.T01:cds;Parent=MsG0480020547.01.T01
Gene Sequence

>MsG0480020547.01.T01

ATGGCTGAAGAACATAGATGCCAAGCTGCTCAACGTCTTTGTGCTAACAACTGCGGTTTCTTTGGAAGTCCTGCCATGCAAGATCTTTGCTCCAAATGTTACCGTGATTTGCAGATGAAGGAACAACGTTCTTCATCTGCAAAATTGGTTTTAAACCAAACGTTGATTCCTCAACAGTCTAATTCTTCTTGTCTAGACACGGAGATTATTCATCCATCTCCACCGTCGGTTGTAGTTTCTTCATCTACACCGACGGTGGAGTTGGTAGCTGCTGCTGGACCTTCGGAGGCTGAACCACCGAAGGTCCAGCCAAATAGGTGTGGGACATGCAGACGGCGCGTGGGGCTGACAGGATTTAAGTGTCGGTGCGGGTTGACGTTGTGTGGGACCCACAGGTATCCGGAGCAGCACGGTTGTGGGTTTGATTTTAAGGGGATGGGAAGGGAGGAGATCAAGAAGGCGAATCCAGTGGTGAAGGGAGAGAAGCTGAATAAGATCTGA

Protein sequence

>MsG0480020547.01.T01

MAEEHRCQAAQRLCANNCGFFGSPAMQDLCSKCYRDLQMKEQRSSSAKLVLNQTLIPQQSNSSCLDTEIIHPSPPSVVVSSSTPTVELVAAAGPSEAEPPKVQPNRCGTCRRRVGLTGFKCRCGLTLCGTHRYPEQHGCGFDFKGMGREEIKKANPVVKGEKLNKI*