AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480020596.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020596.01.T01 MTR_4g055420 95.408 196 9 0 1 196 1 196 3.31e-140 389
MsG0480020596.01.T01 MTR_2g084805 54.857 175 67 5 15 182 8 177 2.66e-56 177
MsG0480020596.01.T01 MTR_4g102040 43.077 130 58 2 69 196 57 172 2.86e-28 104
MsG0480020596.01.T01 MTR_5g011750 51.724 87 42 0 69 155 26 112 7.41e-25 94.0
MsG0480020596.01.T01 MTR_5g011770 40.741 81 48 0 72 152 117 197 1.25e-14 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480020596.01.T01 AT2G27830 47.959 196 94 4 1 194 1 190 5.78e-50 160
MsG0480020596.01.T01 AT4G22758 38.462 91 54 1 68 158 119 207 8.82e-13 65.5
MsG0480020596.01.T01 AT1G70780 30.328 122 79 3 71 188 16 135 8.00e-12 60.8

Find 51 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCACTTTCTTCAAGCTTCTT+TGG 0.205520 4:-44367043 None:intergenic
AATGAGAAGCTTAAGGAAAT+TGG 0.265022 4:+44366998 MsG0480020596.01.T01:CDS
GCCGACGAAGTTACTCATTA+AGG 0.295975 4:+44366533 MsG0480020596.01.T01:CDS
GAGTAACTTCGTCGGCTGTC+TGG 0.327050 4:-44366526 None:intergenic
CACACTTCTGCAACACTGTC+CGG 0.327283 4:+44366414 MsG0480020596.01.T01:CDS
GCTTCCTTCGACGGCAAGAT+TGG 0.327598 4:-44366666 None:intergenic
TTGCCGCACGCTTCCTGTCC+TGG 0.374159 4:-44366476 None:intergenic
TAAACGACGCCGTTACGTTA+TGG 0.389603 4:-44367078 None:intergenic
AGATAGAAATGAGAAGCTTA+AGG 0.392238 4:+44366991 MsG0480020596.01.T01:CDS
ACAAGGTCCGGCATAGTCTT+TGG 0.414724 4:-44366453 None:intergenic
ACACTTCTGCAACACTGTCC+GGG 0.435674 4:+44366415 MsG0480020596.01.T01:CDS
GAGTTTCCACATGGCTGTTA+CGG 0.438950 4:-44366347 None:intergenic
ATTTGATAGCGGAGGCTGTA+AGG 0.439277 4:+44366619 MsG0480020596.01.T01:CDS
ACTCATTAAGGTTACGATGA+TGG 0.455220 4:+44366545 MsG0480020596.01.T01:CDS
CTCATTAAGGTTACGATGAT+GGG 0.455912 4:+44366546 MsG0480020596.01.T01:CDS
AGTAACTTCGTCGGCTGTCT+GGG 0.474432 4:-44366525 None:intergenic
CACTGTCCGGGGCAAAACTC+CGG 0.481170 4:+44366427 MsG0480020596.01.T01:CDS
CACGCTTCCTGTCCTGGACA+AGG 0.482528 4:-44366470 None:intergenic
TTCCTGTCCTGGACAAGGTC+CGG 0.485224 4:-44366465 None:intergenic
GGCAATGACTTCTTCGCCAC+CGG 0.515088 4:+44366494 MsG0480020596.01.T01:CDS
GTTACGATGATGGGCAGCCT+TGG 0.521614 4:+44366555 MsG0480020596.01.T01:CDS
TGCCGGACCTTGTCCAGGAC+AGG 0.523940 4:+44366463 MsG0480020596.01.T01:CDS
GACTATGCCGGACCTTGTCC+AGG 0.528186 4:+44366458 MsG0480020596.01.T01:CDS
CTCTCCGGTGAGTTTCCACA+TGG 0.533759 4:-44366356 None:intergenic
TCTCTGTTAATCTTCCTCTC+CGG 0.534835 4:-44366371 None:intergenic
ATGACGATGGAGTCGACGGT+TGG 0.536486 4:+44366594 MsG0480020596.01.T01:CDS
CCGGCATAGTCTTTGGCCGC+CGG 0.545096 4:-44366446 None:intergenic
AAGCAAGAAGCTTCCTTCGA+CGG 0.546345 4:-44366675 None:intergenic
GTAAGGTTGTATGTTAAAGA+AGG 0.550051 4:+44366636 MsG0480020596.01.T01:CDS
TTTCCACATGGCTGTTACGG+TGG 0.552551 4:-44366344 None:intergenic
CCGTCCAATCTTGCCGTCGA+AGG 0.553306 4:+44366662 MsG0480020596.01.T01:CDS
CATCGTCATTAACACTTGCA+CGG 0.554830 4:-44366580 None:intergenic
ATCGTCATTAACACTTGCAC+GGG 0.561873 4:-44366579 None:intergenic
AGCTTAAGGAAATTGGATCA+AGG 0.569028 4:+44367005 MsG0480020596.01.T01:CDS
CCTTCGACGGCAAGATTGGA+CGG 0.570300 4:-44366662 None:intergenic
CACTTCTGCAACACTGTCCG+GGG 0.582028 4:+44366416 MsG0480020596.01.T01:CDS
GTTAATGACGATGGAGTCGA+CGG 0.597176 4:+44366590 MsG0480020596.01.T01:CDS
CCGGCGGCCAAAGACTATGC+CGG 0.600487 4:+44366446 MsG0480020596.01.T01:CDS
TTAACACTTGCACGGGACCA+AGG 0.603739 4:-44366572 None:intergenic
ACAGCCATGTGGAAACTCAC+CGG 0.607844 4:+44366352 MsG0480020596.01.T01:CDS
CGTGCAAGTGTTAATGACGA+TGG 0.614536 4:+44366581 MsG0480020596.01.T01:CDS
AAGCTTGAAGAAAGTGACAA+TGG 0.627431 4:+44367049 MsG0480020596.01.T01:CDS
ACCTTAATGAGTAACTTCGT+CGG 0.631958 4:-44366534 None:intergenic
GGTTGGAGATTTGATAGCGG+AGG 0.649039 4:+44366611 MsG0480020596.01.T01:CDS
TGTCCAGGACAGGAAGCGTG+CGG 0.659036 4:+44366473 MsG0480020596.01.T01:CDS
CTTGAAGAAAGTGACAATGG+TGG 0.660090 4:+44367052 MsG0480020596.01.T01:CDS
TGGTGGAGACCATAACGTAA+CGG 0.684404 4:+44367069 MsG0480020596.01.T01:CDS
TGTCCGGGGCAAAACTCCGG+CGG 0.695006 4:+44366430 MsG0480020596.01.T01:CDS
CATGTGGAAACTCACCGGAG+AGG 0.696044 4:+44366357 MsG0480020596.01.T01:CDS
AAACCACCGTAACAGCCATG+TGG 0.698169 4:+44366341 MsG0480020596.01.T01:CDS
GACGGTTGGAGATTTGATAG+CGG 0.725699 4:+44366608 MsG0480020596.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGTAGTTATTATTTTTTGT+TGG + Chr4:44366935-44366954 MsG0480020596.01.T01:intron 15.0%
!!! TATTAGGTCTTTATTTTTTT+TGG + Chr4:44366865-44366884 MsG0480020596.01.T01:intron 15.0%
!! TGAACTATGAAAACTAAAAT+TGG - Chr4:44366746-44366765 None:intergenic 20.0%
!!! AGTTATTATTTTTTGTTGGA+GGG + Chr4:44366939-44366958 MsG0480020596.01.T01:intron 20.0%
!!! TAGTTATTATTTTTTGTTGG+AGG + Chr4:44366938-44366957 MsG0480020596.01.T01:intron 20.0%
! ACTAGAACAACGTAAATATT+AGG + Chr4:44366849-44366868 MsG0480020596.01.T01:intron 25.0%
AATGAGAAGCTTAAGGAAAT+TGG + Chr4:44366998-44367017 MsG0480020596.01.T01:CDS 30.0%
AGATAGAAATGAGAAGCTTA+AGG + Chr4:44366991-44367010 MsG0480020596.01.T01:CDS 30.0%
GTAAGGTTGTATGTTAAAGA+AGG + Chr4:44366636-44366655 MsG0480020596.01.T01:CDS 30.0%
TATGACGTATCTAATTTCTC+TGG + Chr4:44366810-44366829 MsG0480020596.01.T01:intron 30.0%
! GAGAAATCATCATCTTTTCA+TGG + Chr4:44366390-44366409 MsG0480020596.01.T01:CDS 30.0%
!!! TTGATTTGTTTTATGAGTGC+AGG + Chr4:44366965-44366984 MsG0480020596.01.T01:intron 30.0%
AAGCTTGAAGAAAGTGACAA+TGG + Chr4:44367049-44367068 MsG0480020596.01.T01:CDS 35.0%
ACCTTAATGAGTAACTTCGT+CGG - Chr4:44366537-44366556 None:intergenic 35.0%
ACTCATTAAGGTTACGATGA+TGG + Chr4:44366545-44366564 MsG0480020596.01.T01:CDS 35.0%
AGCTTAAGGAAATTGGATCA+AGG + Chr4:44367005-44367024 MsG0480020596.01.T01:CDS 35.0%
CTCATTAAGGTTACGATGAT+GGG + Chr4:44366546-44366565 MsG0480020596.01.T01:CDS 35.0%
TCACTTTCTTCAAGCTTCTT+TGG - Chr4:44367046-44367065 None:intergenic 35.0%
! CAAGTTGAAAAAGTGAGCAA+AGG + Chr4:44367115-44367134 MsG0480020596.01.T01:CDS 35.0%
! GAAAAAGTGAGCAAAGGTTT+TGG + Chr4:44367121-44367140 MsG0480020596.01.T01:CDS 35.0%
!! GCAAAGGTTTTGGTCTTTTA+AGG + Chr4:44367131-44367150 MsG0480020596.01.T01:CDS 35.0%
ATCGTCATTAACACTTGCAC+GGG - Chr4:44366582-44366601 None:intergenic 40.0%
CATCGTCATTAACACTTGCA+CGG - Chr4:44366583-44366602 None:intergenic 40.0%
CTTGAAGAAAGTGACAATGG+TGG + Chr4:44367052-44367071 MsG0480020596.01.T01:CDS 40.0%
GTACCATTGTAGATGCTCTT+AGG + Chr4:44366890-44366909 MsG0480020596.01.T01:intron 40.0%
TCTCTGTTAATCTTCCTCTC+CGG - Chr4:44366374-44366393 None:intergenic 40.0%
!! AGACCTAAGAGCATCTACAA+TGG - Chr4:44366896-44366915 None:intergenic 40.0%
!!! GCTCACTTTTTCAACTTGTC+TGG - Chr4:44367113-44367132 None:intergenic 40.0%
AAGCAAGAAGCTTCCTTCGA+CGG - Chr4:44366678-44366697 None:intergenic 45.0%
CGTGCAAGTGTTAATGACGA+TGG + Chr4:44366581-44366600 MsG0480020596.01.T01:CDS 45.0%
GACGGTTGGAGATTTGATAG+CGG + Chr4:44366608-44366627 MsG0480020596.01.T01:CDS 45.0%
GAGTTTCCACATGGCTGTTA+CGG - Chr4:44366350-44366369 None:intergenic 45.0%
GCCGACGAAGTTACTCATTA+AGG + Chr4:44366533-44366552 MsG0480020596.01.T01:CDS 45.0%
GTTAATGACGATGGAGTCGA+CGG + Chr4:44366590-44366609 MsG0480020596.01.T01:CDS 45.0%
TAAACGACGCCGTTACGTTA+TGG - Chr4:44367081-44367100 None:intergenic 45.0%
TGGTGGAGACCATAACGTAA+CGG + Chr4:44367069-44367088 MsG0480020596.01.T01:CDS 45.0%
!! ATTTGATAGCGGAGGCTGTA+AGG + Chr4:44366619-44366638 MsG0480020596.01.T01:CDS 45.0%
AAACCACCGTAACAGCCATG+TGG + Chr4:44366341-44366360 MsG0480020596.01.T01:CDS 50.0%
ACAAGGTCCGGCATAGTCTT+TGG - Chr4:44366456-44366475 None:intergenic 50.0%
ACACTTCTGCAACACTGTCC+GGG + Chr4:44366415-44366434 MsG0480020596.01.T01:CDS 50.0%
ACAGCCATGTGGAAACTCAC+CGG + Chr4:44366352-44366371 MsG0480020596.01.T01:CDS 50.0%
CACACTTCTGCAACACTGTC+CGG + Chr4:44366414-44366433 MsG0480020596.01.T01:CDS 50.0%
GGCTGTCTGGGAAAAAACTC+CGG - Chr4:44366516-44366535 None:intergenic 50.0%
TGTCTGGGAAAAAACTCCGG+TGG - Chr4:44366513-44366532 None:intergenic 50.0%
TTAACACTTGCACGGGACCA+AGG - Chr4:44366575-44366594 None:intergenic 50.0%
TTTCCACATGGCTGTTACGG+TGG - Chr4:44366347-44366366 None:intergenic 50.0%
! AGTAACTTCGTCGGCTGTCT+GGG - Chr4:44366528-44366547 None:intergenic 50.0%
!! GGTTGGAGATTTGATAGCGG+AGG + Chr4:44366611-44366630 MsG0480020596.01.T01:CDS 50.0%
ATGACGATGGAGTCGACGGT+TGG + Chr4:44366594-44366613 MsG0480020596.01.T01:CDS 55.0%
CACTTCTGCAACACTGTCCG+GGG + Chr4:44366416-44366435 MsG0480020596.01.T01:CDS 55.0%
CATGTGGAAACTCACCGGAG+AGG + Chr4:44366357-44366376 MsG0480020596.01.T01:CDS 55.0%
CCTTCGACGGCAAGATTGGA+CGG - Chr4:44366665-44366684 None:intergenic 55.0%
CTCTCCGGTGAGTTTCCACA+TGG - Chr4:44366359-44366378 None:intergenic 55.0%
GCTTCCTTCGACGGCAAGAT+TGG - Chr4:44366669-44366688 None:intergenic 55.0%
GGCAATGACTTCTTCGCCAC+CGG + Chr4:44366494-44366513 MsG0480020596.01.T01:CDS 55.0%
TTCCTGTCCTGGACAAGGTC+CGG - Chr4:44366468-44366487 None:intergenic 55.0%
! GAGTAACTTCGTCGGCTGTC+TGG - Chr4:44366529-44366548 None:intergenic 55.0%
!! GTTACGATGATGGGCAGCCT+TGG + Chr4:44366555-44366574 MsG0480020596.01.T01:CDS 55.0%
CACGCTTCCTGTCCTGGACA+AGG - Chr4:44366473-44366492 None:intergenic 60.0%
CACTGTCCGGGGCAAAACTC+CGG + Chr4:44366427-44366446 MsG0480020596.01.T01:CDS 60.0%
CCGTCCAATCTTGCCGTCGA+AGG + Chr4:44366662-44366681 MsG0480020596.01.T01:CDS 60.0%
GACTATGCCGGACCTTGTCC+AGG + Chr4:44366458-44366477 MsG0480020596.01.T01:CDS 60.0%
! TGTCCAGGACAGGAAGCGTG+CGG + Chr4:44366473-44366492 MsG0480020596.01.T01:CDS 60.0%
CCGGCATAGTCTTTGGCCGC+CGG - Chr4:44366449-44366468 None:intergenic 65.0%
CCGGCGGCCAAAGACTATGC+CGG + Chr4:44366446-44366465 MsG0480020596.01.T01:CDS 65.0%
TGCCGGACCTTGTCCAGGAC+AGG + Chr4:44366463-44366482 MsG0480020596.01.T01:CDS 65.0%
TGTCCGGGGCAAAACTCCGG+CGG + Chr4:44366430-44366449 MsG0480020596.01.T01:CDS 65.0%
TTGCCGCACGCTTCCTGTCC+TGG - Chr4:44366479-44366498 None:intergenic 65.0%
! TGGCCGCCGGAGTTTTGCCC+CGG - Chr4:44366436-44366455 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 44366327 44367174 44366327 ID=MsG0480020596.01;Name=MsG0480020596.01
Chr4 mRNA 44366327 44367174 44366327 ID=MsG0480020596.01.T01;Parent=MsG0480020596.01;Name=MsG0480020596.01.T01;_AED=0.49;_eAED=0.49;_QI=0|0|0|1|1|1|2|0|196
Chr4 exon 44366327 44366729 44366327 ID=MsG0480020596.01.T01:exon:13865;Parent=MsG0480020596.01.T01
Chr4 exon 44366987 44367174 44366987 ID=MsG0480020596.01.T01:exon:13866;Parent=MsG0480020596.01.T01
Chr4 CDS 44366327 44366729 44366327 ID=MsG0480020596.01.T01:cds;Parent=MsG0480020596.01.T01
Chr4 CDS 44366987 44367174 44366987 ID=MsG0480020596.01.T01:cds;Parent=MsG0480020596.01.T01
Gene Sequence

>MsG0480020596.01.T01

ATGTCAAACGTCAAAAACCACCGTAACAGCCATGTGGAAACTCACCGGAGAGGAAGATTAACAGAGAAATCATCATCTTTTCATGGTCACACTTCTGCAACACTGTCCGGGGCAAAACTCCGGCGGCCAAAGACTATGCCGGACCTTGTCCAGGACAGGAAGCGTGCGGCAATGACTTCTTCGCCACCGGAGTTTTTTCCCAGACAGCCGACGAAGTTACTCATTAAGGTTACGATGATGGGCAGCCTTGGTCCCGTGCAAGTGTTAATGACGATGGAGTCGACGGTTGGAGATTTGATAGCGGAGGCTGTAAGGTTGTATGTTAAAGAAGGTCGCCGTCCAATCTTGCCGTCGAAGGAAGCTTCTTGCTTTGATCTTCATTACTCTCAGTTCAGCTTAGAAAGTTTAGATAGAAATGAGAAGCTTAAGGAAATTGGATCAAGGAACTTTTTTATGTGTCCAAAGAAGCTTGAAGAAAGTGACAATGGTGGAGACCATAACGTAACGGCGTCGTTTAGTTCATGTTCCAGACAAGTTGAAAAAGTGAGCAAAGGTTTTGGTCTTTTAAGGTGTATAGATTTCTTGATATGA

Protein sequence

>MsG0480020596.01.T01

MSNVKNHRNSHVETHRRGRLTEKSSSFHGHTSATLSGAKLRRPKTMPDLVQDRKRAAMTSSPPEFFPRQPTKLLIKVTMMGSLGPVQVLMTMESTVGDLIAEAVRLYVKEGRRPILPSKEASCFDLHYSQFSLESLDRNEKLKEIGSRNFFMCPKKLEESDNGGDHNVTASFSSCSRQVEKVSKGFGLLRCIDFLI*