Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020938.01.T01 | XP_013456099.1 | 79.195 | 149 | 24 | 1 | 40 | 188 | 94 | 235 | 6.63E-65 | 222 |
| MsG0480020938.01.T01 | XP_013456099.1 | 54.61 | 141 | 33 | 3 | 198 | 332 | 329 | 444 | 6.42E-30 | 129 |
| MsG0480020938.01.T02 | XP_013456099.1 | 96 | 50 | 2 | 0 | 40 | 89 | 94 | 143 | 9.90E-22 | 99.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020938.01.T01 | A0A2H5ND67 | 55.088 | 285 | 56 | 5 | 40 | 285 | 105 | 356 | 4.61e-82 | 264 |
| MsG0480020938.01.T02 | A0A072UW90 | 96.000 | 50 | 2 | 0 | 40 | 89 | 94 | 143 | 4.73e-22 | 99.4 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047910.01 | MsG0480020938.01 | 0.838285 | 3.149247e-57 | 4.197988e-54 |
| MsG0080047967.01 | MsG0480020938.01 | 0.850385 | 1.756847e-60 | 3.435474e-57 |
| MsG0080048059.01 | MsG0480020938.01 | 0.846348 | 2.298934e-59 | 3.945308e-56 |
| MsG0080048274.01 | MsG0480020938.01 | 0.805455 | 1.323673e-49 | 7.006193e-47 |
| MsG0080048486.01 | MsG0480020938.01 | -0.811407 | 7.110061e-51 | 4.397714e-48 |
| MsG0080048487.01 | MsG0480020938.01 | -0.809428 | 1.901086e-50 | 1.115922e-47 |
| MsG0080048488.01 | MsG0480020938.01 | -0.809669 | 1.687865e-50 | 9.971420e-48 |
| MsG0080048489.01 | MsG0480020938.01 | -0.808934 | 2.427004e-50 | 1.405801e-47 |
| MsG0080048611.01 | MsG0480020938.01 | 0.829631 | 4.621124e-55 | 4.756275e-52 |
| MsG0080048694.01 | MsG0480020938.01 | 0.822017 | 2.966304e-53 | 2.452976e-50 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020938.01.T01 | MTR_4g063705 | 79.195 | 149 | 24 | 1 | 40 | 188 | 94 | 235 | 8.03e-69 | 222 |
| MsG0480020938.01.T01 | MTR_4g063705 | 54.610 | 141 | 33 | 3 | 198 | 332 | 329 | 444 | 7.78e-34 | 129 |
| MsG0480020938.01.T01 | MTR_3g086250 | 56.954 | 151 | 50 | 2 | 42 | 188 | 109 | 248 | 6.83e-50 | 174 |
| MsG0480020938.01.T02 | MTR_4g063705 | 96.000 | 50 | 2 | 0 | 40 | 89 | 94 | 143 | 1.20e-25 | 99.4 |
| MsG0480020938.01.T02 | MTR_3g086250 | 59.259 | 54 | 22 | 0 | 42 | 95 | 109 | 162 | 1.60e-16 | 73.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480020938.01.T01 | AT2G28800 | 76.000 | 150 | 27 | 2 | 40 | 188 | 114 | 255 | 2.09e-66 | 213 |
| MsG0480020938.01.T01 | AT2G28800 | 76.000 | 150 | 27 | 2 | 40 | 188 | 114 | 255 | 2.56e-66 | 213 |
| MsG0480020938.01.T01 | AT2G28800 | 76.000 | 150 | 27 | 2 | 40 | 188 | 114 | 255 | 7.85e-66 | 214 |
| MsG0480020938.01.T01 | AT1G24490 | 64.238 | 151 | 39 | 2 | 42 | 188 | 100 | 239 | 2.57e-56 | 191 |
| MsG0480020938.01.T01 | AT2G28800 | 56.376 | 149 | 28 | 2 | 40 | 188 | 114 | 225 | 1.20e-38 | 142 |
| MsG0480020938.01.T01 | AT1G24490 | 58.095 | 105 | 29 | 2 | 88 | 188 | 109 | 202 | 2.90e-29 | 117 |
| MsG0480020938.01.T02 | AT2G28800 | 90.000 | 50 | 5 | 0 | 40 | 89 | 114 | 163 | 6.30e-24 | 93.6 |
| MsG0480020938.01.T02 | AT2G28800 | 90.000 | 50 | 5 | 0 | 40 | 89 | 114 | 163 | 6.47e-24 | 93.6 |
| MsG0480020938.01.T02 | AT2G28800 | 90.000 | 50 | 5 | 0 | 40 | 89 | 114 | 163 | 7.05e-24 | 94.4 |
| MsG0480020938.01.T02 | AT2G28800 | 90.000 | 50 | 5 | 0 | 40 | 89 | 114 | 163 | 8.81e-24 | 94.4 |
Find 78 sgRNAs with CRISPR-Local
Find 387 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTTCAACCTAAAATTA+AGG | 0.326230 | 4:-50793855 | MsG0480020938.01.T01:CDS |
| AAATACAACTTGAGACTTCA+AGG | 0.459373 | 4:-50793679 | MsG0480020938.01.T01:CDS |
| AACACCCCTCACCTGATTGC+CGG | 0.401631 | 4:+50793815 | None:intergenic |
| AAGAAGATATGCCGGCAATC+AGG | 0.452072 | 4:-50793826 | MsG0480020938.01.T01:intron |
| AAGCCCGAAAGGATGGTGAA+AGG | 0.642072 | 4:-50790946 | MsG0480020938.01.T01:CDS |
| AATAGGTTGCGCAAATTAGG+AGG | 0.569780 | 4:-50791039 | MsG0480020938.01.T01:intron |
| AATTTACATTTGCTAACATG+TGG | 0.530000 | 4:+50789959 | None:intergenic |
| AATTTGCCATAAGTATATGA+GGG | 0.622193 | 4:+50790883 | None:intergenic |
| ACAAATAGGTTGCGCAAATT+AGG | 0.308233 | 4:-50791042 | MsG0480020938.01.T01:intron |
| ACGAGGTTATCATAATTTCA+TGG | 0.436848 | 4:+50794039 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATACAACTTGAGACTTCA+AGG | - | Chr4:50791474-50791493 | MsG0480020938.01.T01:intron | 30.0% | |
| AACATTTGCCATATCATAAC+TGG | - | Chr4:50791620-50791639 | MsG0480020938.01.T01:intron | 30.0% | |
| AATAGATGGTAGTTGGAATT+CGG | + | Chr4:50794616-50794635 | None:intergenic | 30.0% | |
| ACAAGCAAAATGATTACTAG+GGG | + | Chr4:50792927-50792946 | None:intergenic | 30.0% | |
| ACATTTGCCATATCATAACT+GGG | - | Chr4:50791621-50791640 | MsG0480020938.01.T01:intron | 30.0% | |
| ACGAGGTTATCATAATTTCA+TGG | + | Chr4:50791117-50791136 | None:intergenic | 30.0% | |
| ACTTAGATACTTCTTTCTGA+AGG | - | Chr4:50793914-50793933 | MsG0480020938.01.T01:intron | 30.0% | |
| AGAAAAACTTTGAACCTCTT+GGG | + | Chr4:50794998-50795017 | None:intergenic | 30.0% | |
| AGATTGAAATAGATGGTAGT+TGG | + | Chr4:50794623-50794642 | None:intergenic | 30.0% | |
| AGTAAATGTAACTAGGAAGT+AGG | - | Chr4:50793036-50793055 | MsG0480020938.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 50789815 | 50795360 | 50789815 | ID=MsG0480020938.01;Name=MsG0480020938.01 |
| Chr4 | mRNA | 50789815 | 50795360 | 50789815 | ID=MsG0480020938.01.T01;Parent=MsG0480020938.01;Name=MsG0480020938.01.T01;_AED=0.49;_eAED=0.54;_QI=267|0.42|0.75|1|0.71|0.62|8|298|332 |
| Chr4 | exon | 50789815 | 50790218 | 50789815 | ID=MsG0480020938.01.T01:exon:1056;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50790763 | 50791056 | 50790763 | ID=MsG0480020938.01.T01:exon:1055;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50793223 | 50793326 | 50793223 | ID=MsG0480020938.01.T01:exon:1054;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50793421 | 50793494 | 50793421 | ID=MsG0480020938.01.T01:exon:1053;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50793662 | 50793744 | 50793662 | ID=MsG0480020938.01.T01:exon:1052;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50793827 | 50793900 | 50793827 | ID=MsG0480020938.01.T01:exon:1051;Parent=MsG0480020938.01.T01 |
| Chr4 | exon | 50794105 | 50794215 | 50794105 | ID=MsG0480020938.01.T01:exon:1050;Parent=MsG0480020938.01.T01 |
| Chr4 | five_prime_UTR | 50795094 | 50795360 | 50795094 | ID=MsG0480020938.01.T01:five_prime_utr;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50794941 | 50795093 | 50794941 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50794105 | 50794215 | 50794105 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50793827 | 50793900 | 50793827 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50793662 | 50793744 | 50793662 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50793421 | 50793494 | 50793421 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50793223 | 50793326 | 50793223 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50790763 | 50791056 | 50790763 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | CDS | 50790113 | 50790218 | 50790113 | ID=MsG0480020938.01.T01:cds;Parent=MsG0480020938.01.T01 |
| Chr4 | three_prime_UTR | 50789815 | 50790112 | 50789815 | ID=MsG0480020938.01.T01:three_prime_utr;Parent=MsG0480020938.01.T01 |
| Chr4 | mRNA | 50794048 | 50795360 | 50794048 | ID=MsG0480020938.01.T02;Parent=MsG0480020938.01;Name=MsG0480020938.01.T02;_AED=0.50;_eAED=0.53;_QI=267|1|0.5|1|1|1|2|0|106 |
| Chr4 | exon | 50794048 | 50794215 | 50794048 | ID=MsG0480020938.01.T02:exon:1057;Parent=MsG0480020938.01.T02 |
| Chr4 | five_prime_UTR | 50795094 | 50795360 | 50795094 | ID=MsG0480020938.01.T02:five_prime_utr;Parent=MsG0480020938.01.T02 |
| Chr4 | CDS | 50794941 | 50795093 | 50794941 | ID=MsG0480020938.01.T02:cds;Parent=MsG0480020938.01.T02 |
| Chr4 | CDS | 50794048 | 50794215 | 50794048 | ID=MsG0480020938.01.T02:cds;Parent=MsG0480020938.01.T02 |
| Gene Sequence |
>MsG0480020938.01.T01 ATGTTGCCGGTGTCTTCGCCAGAGCTGAAGGACTTCTTTATACGCTCGCCGACGCTGCCGTTACCGCCGATGCAGTTACTTCCACGGATGTTGCTGTGCAGAAGAACGGAGTTGGTTGGTTTTATCTCCGATGCCATGGAGTTTGTTCTTAAGATATTAAAGGATGGTTTGTCTGCCGTGCATGTGCCTTATTCGTATGGATTTGCTATCATATTGCTCACTCTTATTGTTAAGGCCGCTACACTTCCTTTGACAAAGCAACAGGTTGAATCAACACTAGCTATGCAAAACCTTCAACCTAAAATTAAGGCCATTCAAGAAGATATGCCGGCAATCAGCATGGTTGTTCTAACTACTTATTCATTGCCCTCATTTCAAGGAAAATACAACTTGAGACTTCAAGGCTATATACCCAAGCTGGGTTGTTTACCGACTTTGGCTACTATTCCCGTCTGGATTGGTCTATACCAAGCTTTATCTAATGTGGCAAATGAGGGACTGTTAACAGAAGGTTTCTTATGGATCCCTTCTCTCGGTGGTCCCACTAGCATTGCTGCTAGACAAGTGGATCCGGAATTTCTTGGCTTTTTTCATTTGTGGTTGCGCAAATTAGGAGGTGCAAAGCCTGCTGTTAATGAAGATGCTGGTGGAATTATTACGGCAGGACGAGCTAAGAGATCATTTTCTAAGCCCGAAAGGATGGTGAAAGGTCAGTATTTTACGATACAGGTTGCGCCTCAGCCTTTCAATCCCTCATATACTTATGGCAAATTGGTGATTTACAGGTTTACGCAGTTAAAAGAAGAGGAGAAGAAGAAAAAGTTAAGCAAGGCACTGCCCGTGGATGAAGTTCAACTCTGGCTTCGGCTTCTGTTTCCGATGATGGCTCGGATGACAAGAAGTTACCGAAGAGGCGTATGCATCCAAGTTCCCCAAGAGGTTCAAAGTTTTTCTAGGGAAAGAAGAAGCAAGCGTTCAAAGCGAAAGCCTGTTGTATGA >MsG0480020938.01.T02 ATGTTGCCGGTGTCTTCGCCAGAGCTGAAGGACTTCTTTATACGCTCGCCGACGCTGCCGTTACCGCCGATGCAGTTACTTCCACGGATGTTGCTGTGCAGAAGAACGGAGTTGGTTGGTTTTATCTCCGATGCCATGGAGTTTGTTCTTAAGATATTAAAGGATGGTTTGTCTGCCGTGCATGTGCCTTATTCGTATGGATTTGCTATCATATTGCTCACTCTTATTGTTAAGGCCGCTACACTTCCTTTGACAAAGCAACAGTTAGTACAAATTACAGCAATTTCTATAGGCAAATTTACTGAAACCATGAAATTATGA |
| Protein sequence |
>MsG0480020938.01.T01 MLPVSSPELKDFFIRSPTLPLPPMQLLPRMLLCRRTELVGFISDAMEFVLKILKDGLSAVHVPYSYGFAIILLTLIVKAATLPLTKQQVESTLAMQNLQPKIKAIQEDMPAISMVVLTTYSLPSFQGKYNLRLQGYIPKLGCLPTLATIPVWIGLYQALSNVANEGLLTEGFLWIPSLGGPTSIAARQVDPEFLGFFHLWLRKLGGAKPAVNEDAGGIITAGRAKRSFSKPERMVKGQYFTIQVAPQPFNPSYTYGKLVIYRFTQLKEEEKKKKLSKALPVDEVQLWLRLLFPMMARMTRSYRRGVCIQVPQEVQSFSRERRSKRSKRKPVV* >MsG0480020938.01.T02 MLPVSSPELKDFFIRSPTLPLPPMQLLPRMLLCRRTELVGFISDAMEFVLKILKDGLSAVHVPYSYGFAIILLTLIVKAATLPLTKQQLVQITAISIGKFTETMKL* |
