AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021775.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021775.01.T01 MTR_7g062720 61.728 81 31 0 1 81 54 134 2.21e-29 109
MsG0480021775.01.T01 MTR_5g061150 64.557 79 28 0 1 79 44 122 7.82e-29 107
MsG0480021775.01.T01 MTR_1g036690 62.295 61 17 2 183 243 5 59 5.42e-18 76.6
MsG0480021775.01.T01 MTR_4g037355 41.791 134 51 6 61 190 6 116 2.87e-17 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTTCAAAATAAGGATAAA+AGG 0.234592 4:-64156731 MsG0480021775.01.T01:CDS
CCAACAATCACTTCAAAATA+AGG 0.244294 4:-64156739 MsG0480021775.01.T01:CDS
ATGGTTATATTTGCGTTTAC+AGG 0.259438 4:-64157348 MsG0480021775.01.T01:CDS
AAATTGTCCCAAAGATCTTT+TGG 0.264548 4:+64157425 None:intergenic
TAGAAGATCCAAAAGATCTT+TGG 0.283634 4:-64157433 MsG0480021775.01.T01:CDS
TGTGTTGCTACATTCACTTC+TGG 0.294995 4:+64157059 None:intergenic
TATAGGTGGGGTTCTATTCC+TGG 0.331534 4:+64156580 None:intergenic
TAAAGATCAACAAGATGTTT+TGG 0.333763 4:+64156762 None:intergenic
TGAAGATGATTATGGTAATA+TGG 0.338642 4:-64156679 MsG0480021775.01.T01:CDS
AACCATTCATTACGAGCATT+AGG 0.386252 4:+64157365 None:intergenic
GAAAATGATGAAGATGATTA+TGG 0.391347 4:-64156687 MsG0480021775.01.T01:CDS
AGAAGATCCAAAAGATCTTT+GGG 0.400056 4:-64157432 MsG0480021775.01.T01:CDS
GTATGGAAGGGGACGTGGTT+TGG 0.405914 4:-64156966 MsG0480021775.01.T01:CDS
TGCTATCGTTGTGGAGGAAA+AGG 0.414829 4:-64156810 MsG0480021775.01.T01:CDS
CTTTGGGACAATTTAAAGAA+AGG 0.419598 4:-64157416 MsG0480021775.01.T01:CDS
GTGTTGCTACATTCACTTCT+GGG 0.423307 4:+64157060 None:intergenic
CCTTCGTCACCACCTTCATA+AGG 0.433246 4:-64157480 MsG0480021775.01.T01:CDS
GACGTGGACGTGGCGTGGTA+TGG 0.435978 4:-64156983 MsG0480021775.01.T01:CDS
ATTAACACTGTGTGGAGAAA+AGG 0.440407 4:-64157273 MsG0480021775.01.T01:intron
CAAAATCGTGGTCGTGGCCG+TGG 0.442209 4:-64157023 MsG0480021775.01.T01:CDS
CGTGGTCGTGGCCGTGGTCA+TGG 0.454221 4:-64157017 MsG0480021775.01.T01:CDS
TTTGGGACAATTTAAAGAAA+GGG 0.461394 4:-64157415 MsG0480021775.01.T01:CDS
AATGTTGTAAAGAATGATAT+TGG 0.471241 4:-64156861 MsG0480021775.01.T01:CDS
GCGTGGTATGGAAGGGGACG+TGG 0.474989 4:-64156971 MsG0480021775.01.T01:CDS
AAGAATCAGCAGCATCTAAG+TGG 0.476282 4:+64156649 None:intergenic
GCTACATTCACTTCTGGGAA+TGG 0.483138 4:+64157065 None:intergenic
TGAGATGATCAATCTTCATC+AGG 0.485258 4:+64156619 None:intergenic
ACTTCAAAATAAGGATAAAA+GGG 0.487291 4:-64156730 MsG0480021775.01.T01:CDS
GGACAATTTAAAGAAAGGGT+TGG 0.493431 4:-64157411 MsG0480021775.01.T01:CDS
TGGACGTGGCGTGGTATGGA+AGG 0.493864 4:-64156979 MsG0480021775.01.T01:CDS
GTAGCAACACATGATCACTA+TGG 0.504040 4:-64157047 MsG0480021775.01.T01:CDS
GTTGTGGAGGAAAAGGTCAT+TGG 0.504176 4:-64156803 MsG0480021775.01.T01:CDS
CGTGGCCGTGGTCATGGTCG+TGG 0.506333 4:-64157011 MsG0480021775.01.T01:CDS
TTCCTAATGCTCGTAATGAA+TGG 0.506622 4:-64157367 MsG0480021775.01.T01:CDS
GGACGTGGCGTGGTATGGAA+GGG 0.514845 4:-64156978 MsG0480021775.01.T01:CDS
CGTGGACGTGGACGTGGACG+TGG 0.519603 4:-64156993 MsG0480021775.01.T01:CDS
CATGGTCGTGGACGTGGACG+TGG 0.524744 4:-64156999 MsG0480021775.01.T01:CDS
CATTAGAGATGAAAATATCC+AGG 0.532865 4:-64156598 MsG0480021775.01.T01:CDS
GACGTGGCGTGGTATGGAAG+GGG 0.539952 4:-64156977 MsG0480021775.01.T01:CDS
CGTGGTCATGGTCGTGGACG+TGG 0.540833 4:-64157005 MsG0480021775.01.T01:CDS
TGGAGCACTACCAGTGGGAC+GGG 0.544221 4:+64157085 None:intergenic
TCACTCGGCTCCCGTCCCAC+TGG 0.549401 4:-64157095 MsG0480021775.01.T01:CDS
ATGGAGCACTACCAGTGGGA+CGG 0.569862 4:+64157084 None:intergenic
TATGGACAAAATCGTGGTCG+TGG 0.573891 4:-64157029 MsG0480021775.01.T01:CDS
GTCTGTAATCGAATATAACT+CGG 0.574961 4:-64157318 MsG0480021775.01.T01:CDS
GAGAGTATTTGCTATCGTTG+TGG 0.583351 4:-64156819 MsG0480021775.01.T01:CDS
ATTCATTACGAGCATTAGGA+AGG 0.589984 4:+64157369 None:intergenic
CCTTATGAAGGTGGTGACGA+AGG 0.598722 4:+64157480 None:intergenic
AGTATTTGCTATCGTTGTGG+AGG 0.599695 4:-64156816 MsG0480021775.01.T01:CDS
ACGTGGACGTGGACGTGGCG+TGG 0.619346 4:-64156988 MsG0480021775.01.T01:CDS
AGAATCAGCAGCATCTAAGT+GGG 0.645955 4:+64156650 None:intergenic
CACGTCCACGACCATGACCA+CGG 0.661673 4:+64157006 None:intergenic
GTGGTGACGAAGGAATATCA+TGG 0.663314 4:+64157490 None:intergenic
ACTTCTCTATTAACACTGTG+TGG 0.668278 4:-64157281 MsG0480021775.01.T01:CDS
TGGGAATGGAGCACTACCAG+TGG 0.672955 4:+64157079 None:intergenic
GGGAATGGAGCACTACCAGT+GGG 0.676344 4:+64157080 None:intergenic
GATCACTATGGACAAAATCG+TGG 0.728183 4:-64157035 MsG0480021775.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTTGTAAAGAATGATAT+TGG - Chr4:64157220-64157239 MsG0480021775.01.T01:intron 20.0%
!! ACTTCAAAATAAGGATAAAA+GGG - Chr4:64157351-64157370 MsG0480021775.01.T01:CDS 20.0%
! CACTTCAAAATAAGGATAAA+AGG - Chr4:64157350-64157369 MsG0480021775.01.T01:CDS 25.0%
! GAAAATGATGAAGATGATTA+TGG - Chr4:64157394-64157413 MsG0480021775.01.T01:CDS 25.0%
! TGAAGATGATTATGGTAATA+TGG - Chr4:64157402-64157421 MsG0480021775.01.T01:CDS 25.0%
!! TAAAGATCAACAAGATGTTT+TGG + Chr4:64157322-64157341 None:intergenic 25.0%
!! TTACAACATTTTTCATCTTC+TGG + Chr4:64157210-64157229 None:intergenic 25.0%
!! TTTGGGACAATTTAAAGAAA+GGG - Chr4:64156666-64156685 MsG0480021775.01.T01:CDS 25.0%
!!! CATTTTTTAGATCCTTATGA+AGG + Chr4:64156616-64156635 None:intergenic 25.0%
!!! GAGAAAATGTGTTTTTGTTT+TGG + Chr4:64157186-64157205 None:intergenic 25.0%
AGAAGATCCAAAAGATCTTT+GGG - Chr4:64156649-64156668 MsG0480021775.01.T01:CDS 30.0%
ATGGTTATATTTGCGTTTAC+AGG - Chr4:64156733-64156752 MsG0480021775.01.T01:CDS 30.0%
CATTAGAGATGAAAATATCC+AGG - Chr4:64157483-64157502 MsG0480021775.01.T01:CDS 30.0%
CCAACAATCACTTCAAAATA+AGG - Chr4:64157342-64157361 MsG0480021775.01.T01:CDS 30.0%
GTCTGTAATCGAATATAACT+CGG - Chr4:64156763-64156782 MsG0480021775.01.T01:CDS 30.0%
TACATTAGATGCATGAAAAG+TGG + Chr4:64156866-64156885 None:intergenic 30.0%
TAGAAGATCCAAAAGATCTT+TGG - Chr4:64156648-64156667 MsG0480021775.01.T01:CDS 30.0%
! AAATTGTCCCAAAGATCTTT+TGG + Chr4:64156659-64156678 None:intergenic 30.0%
! AAGATCTTTTGGATCTTCTA+TGG + Chr4:64156648-64156667 None:intergenic 30.0%
! AGCTTTGATGAAAAATCACT+CGG - Chr4:64156971-64156990 MsG0480021775.01.T01:CDS 30.0%
! TTTTTAGATCCTTATGAAGG+TGG + Chr4:64156613-64156632 None:intergenic 30.0%
!! CTTTGGGACAATTTAAAGAA+AGG - Chr4:64156665-64156684 MsG0480021775.01.T01:CDS 30.0%
!! GGTTTTAATCGTAATCACAA+TGG - Chr4:64157136-64157155 MsG0480021775.01.T01:intron 30.0%
!!! ACCTTTTAAAACCCTTTTCA+CGG + Chr4:64156906-64156925 None:intergenic 30.0%
!!! CCTTATTTTGAAGTGATTGT+TGG + Chr4:64157345-64157364 None:intergenic 30.0%
AACCATTCATTACGAGCATT+AGG + Chr4:64156719-64156738 None:intergenic 35.0%
ACTTCTCTATTAACACTGTG+TGG - Chr4:64156800-64156819 MsG0480021775.01.T01:CDS 35.0%
ATTAACACTGTGTGGAGAAA+AGG - Chr4:64156808-64156827 MsG0480021775.01.T01:CDS 35.0%
ATTCATTACGAGCATTAGGA+AGG + Chr4:64156715-64156734 None:intergenic 35.0%
GGACAATTTAAAGAAAGGGT+TGG - Chr4:64156670-64156689 MsG0480021775.01.T01:CDS 35.0%
TGAGATGATCAATCTTCATC+AGG + Chr4:64157465-64157484 None:intergenic 35.0%
TTCCTAATGCTCGTAATGAA+TGG - Chr4:64156714-64156733 MsG0480021775.01.T01:CDS 35.0%
! CTTTTGGATCTTCTATGGTA+AGG + Chr4:64156643-64156662 None:intergenic 35.0%
!!! ACCGTGAAAAGGGTTTTAAA+AGG - Chr4:64156902-64156921 MsG0480021775.01.T01:CDS 35.0%
AAGAATCAGCAGCATCTAAG+TGG + Chr4:64157435-64157454 None:intergenic 40.0%
AGAATCAGCAGCATCTAAGT+GGG + Chr4:64157434-64157453 None:intergenic 40.0%
CAGCAACAATACCGTGAAAA+GGG - Chr4:64156892-64156911 MsG0480021775.01.T01:CDS 40.0%
CTTAATATCATGCCTGCTTG+TGG - Chr4:64156933-64156952 MsG0480021775.01.T01:CDS 40.0%
GATCACTATGGACAAAATCG+TGG - Chr4:64157046-64157065 MsG0480021775.01.T01:CDS 40.0%
GTAGCAACACATGATCACTA+TGG - Chr4:64157034-64157053 MsG0480021775.01.T01:CDS 40.0%
! GAAGGATGACAGTTTTCTGA+TGG + Chr4:64156697-64156716 None:intergenic 40.0%
! GTGTTGCTACATTCACTTCT+GGG + Chr4:64157024-64157043 None:intergenic 40.0%
! TATTTTGTTCTGCCACAAGC+AGG + Chr4:64156948-64156967 None:intergenic 40.0%
! TGTGTTGCTACATTCACTTC+TGG + Chr4:64157025-64157044 None:intergenic 40.0%
!! AGTATTTGCTATCGTTGTGG+AGG - Chr4:64157265-64157284 MsG0480021775.01.T01:intron 40.0%
!! GAGAGTATTTGCTATCGTTG+TGG - Chr4:64157262-64157281 MsG0480021775.01.T01:intron 40.0%
GCAGCAACAATACCGTGAAA+AGG - Chr4:64156891-64156910 MsG0480021775.01.T01:CDS 45.0%
GTTGTGGAGGAAAAGGTCAT+TGG - Chr4:64157278-64157297 MsG0480021775.01.T01:CDS 45.0%
TATGGACAAAATCGTGGTCG+TGG - Chr4:64157052-64157071 MsG0480021775.01.T01:CDS 45.0%
TGCTATCGTTGTGGAGGAAA+AGG - Chr4:64157271-64157290 MsG0480021775.01.T01:intron 45.0%
TTTCACGGTATTGTTGCTGC+AGG + Chr4:64156891-64156910 None:intergenic 45.0%
! GCTACATTCACTTCTGGGAA+TGG + Chr4:64157019-64157038 None:intergenic 45.0%
!!! AAGATGTTTTGGCGTACGAC+AGG + Chr4:64157311-64157330 None:intergenic 45.0%
CCTTCGTCACCACCTTCATA+AGG - Chr4:64156601-64156620 MsG0480021775.01.T01:CDS 50.0%
! CCTTATGAAGGTGGTGACGA+AGG + Chr4:64156604-64156623 None:intergenic 50.0%
GTATGGAAGGGGACGTGGTT+TGG - Chr4:64157115-64157134 MsG0480021775.01.T01:CDS 55.0%
! ATGGAGCACTACCAGTGGGA+CGG + Chr4:64157000-64157019 None:intergenic 55.0%
! GGGAATGGAGCACTACCAGT+GGG + Chr4:64157004-64157023 None:intergenic 55.0%
! TGGGAATGGAGCACTACCAG+TGG + Chr4:64157005-64157024 None:intergenic 55.0%
CAAAATCGTGGTCGTGGCCG+TGG - Chr4:64157058-64157077 MsG0480021775.01.T01:CDS 60.0%
CACGTCCACGACCATGACCA+CGG + Chr4:64157078-64157097 None:intergenic 60.0%
GACGTGGCGTGGTATGGAAG+GGG - Chr4:64157104-64157123 MsG0480021775.01.T01:CDS 60.0%
GGACGTGGCGTGGTATGGAA+GGG - Chr4:64157103-64157122 MsG0480021775.01.T01:CDS 60.0%
TGGACGTGGCGTGGTATGGA+AGG - Chr4:64157102-64157121 MsG0480021775.01.T01:CDS 60.0%
! TGGAGCACTACCAGTGGGAC+GGG + Chr4:64156999-64157018 None:intergenic 60.0%
CATGGTCGTGGACGTGGACG+TGG - Chr4:64157082-64157101 MsG0480021775.01.T01:CDS 65.0%
CGTGGTCATGGTCGTGGACG+TGG - Chr4:64157076-64157095 MsG0480021775.01.T01:CDS 65.0%
GACGTGGACGTGGCGTGGTA+TGG - Chr4:64157098-64157117 MsG0480021775.01.T01:CDS 65.0%
GCGTGGTATGGAAGGGGACG+TGG - Chr4:64157110-64157129 MsG0480021775.01.T01:CDS 65.0%
ACGTGGACGTGGACGTGGCG+TGG - Chr4:64157093-64157112 MsG0480021775.01.T01:CDS 70.0%
CGTGGACGTGGACGTGGACG+TGG - Chr4:64157088-64157107 MsG0480021775.01.T01:CDS 70.0%
CGTGGCCGTGGTCATGGTCG+TGG - Chr4:64157070-64157089 MsG0480021775.01.T01:CDS 70.0%
CGTGGTCGTGGCCGTGGTCA+TGG - Chr4:64157064-64157083 MsG0480021775.01.T01:CDS 70.0%
TCACTCGGCTCCCGTCCCAC+TGG - Chr4:64156986-64157005 MsG0480021775.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 64156593 64157510 64156593 ID=MsG0480021775.01;Name=MsG0480021775.01
Chr4 mRNA 64156593 64157510 64156593 ID=MsG0480021775.01.T01;Parent=MsG0480021775.01;Name=MsG0480021775.01.T01;_AED=0.33;_eAED=0.48;_QI=0|0|0|1|1|1|2|0|262
Chr4 exon 64157274 64157510 64157274 ID=MsG0480021775.01.T01:exon:20393;Parent=MsG0480021775.01.T01
Chr4 exon 64156593 64157144 64156593 ID=MsG0480021775.01.T01:exon:20392;Parent=MsG0480021775.01.T01
Chr4 CDS 64157274 64157510 64157274 ID=MsG0480021775.01.T01:cds;Parent=MsG0480021775.01.T01
Chr4 CDS 64156593 64157144 64156593 ID=MsG0480021775.01.T01:cds;Parent=MsG0480021775.01.T01
Gene Sequence

>MsG0480021775.01.T01

ATGATATTCCTTCGTCACCACCTTCATAAGGATCTAAAAAATGAATACCTTACCATAGAAGATCCAAAAGATCTTTGGGACAATTTAAAGAAAGGGTTGGACCATCAGAAAACTGTCATCCTTCCTAATGCTCGTAATGAATGGTTATATTTGCGTTTACAGGACTTTAAGTCTGTAATCGAATATAACTCGGCTGTATTTAAGATTACTTCTCTATTAACACTGTGTGGAGAAAAGAACAAAATAACGAGCTTTGATGAAAAATCACTCGGCTCCCGTCCCACTGGTAGTGCTCCATTCCCAGAAGTGAATGTAGCAACACATGATCACTATGGACAAAATCGTGGTCGTGGCCGTGGTCATGGTCGTGGACGTGGACGTGGACGTGGCGTGGTATGGAAGGGGACGTGGTTTGGTTTTAATCGTAATCACAATGGAGATTACAAGAACACATACTCTCACCAAAACAAAAACACATTTTCTCACCAGAAGATGAAAAATGTTGTAAAGAATGATATTGGTCAAAGCAGCAAAAGTAATGAGAGTATTTGCTATCGTTGTGGAGGAAAAGGTCATTGGAGTCGTACCTGTCGTACGCCAAAACATCTTGTTGATCTTTACCAACAATCACTTCAAAATAAGGATAAAAGGGCTGAAACGCACTTTGCATGCGAAAATGATGAAGATGATTATGGTAATATGGAAAGTACCCACTTAGATGCTGCTGATTCTTTGTTGATCCTGATGAAGATTGATCATCTCATTAGAGATGAAAATATCCAGGAATAG

Protein sequence

>MsG0480021775.01.T01

MIFLRHHLHKDLKNEYLTIEDPKDLWDNLKKGLDHQKTVILPNARNEWLYLRLQDFKSVIEYNSAVFKITSLLTLCGEKNKITSFDEKSLGSRPTGSAPFPEVNVATHDHYGQNRGRGRGHGRGRGRGRGVVWKGTWFGFNRNHNGDYKNTYSHQNKNTFSHQKMKNVVKNDIGQSSKSNESICYRCGGKGHWSRTCRTPKHLVDLYQQSLQNKDKRAETHFACENDEDDYGNMESTHLDAADSLLILMKIDHLIRDENIQE*