AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021738.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021738.01.T01 MTR_4g074370 75.532 188 4 4 38 217 358 511 3.48e-68 228
MsG0480021738.01.T01 MTR_4g074370 97.674 43 1 0 322 364 511 553 2.33e-18 87.4
MsG0480021738.01.T01 MTR_4g108040 73.770 183 6 4 43 217 19 167 6.35e-68 218
MsG0480021738.01.T01 MTR_4g108040 97.674 43 1 0 322 364 167 209 1.93e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021738.01.T01 AT1G50260 65.000 180 29 2 38 217 344 489 7.90e-65 219
MsG0480021738.01.T01 AT1G50260 68.116 69 19 2 317 385 485 550 9.40e-19 88.6
MsG0480021738.01.T01 AT3G19830 60.938 192 40 3 38 229 356 512 1.77e-64 218
MsG0480021738.01.T01 AT3G19830 70.312 64 19 0 322 385 501 564 6.42e-20 92.0
MsG0480021738.01.T01 AT3G19830 60.938 192 40 3 38 229 356 512 1.77e-64 218
MsG0480021738.01.T01 AT3G19830 70.312 64 19 0 322 385 501 564 6.42e-20 92.0
MsG0480021738.01.T01 AT3G19830 60.938 192 40 3 38 229 356 512 1.77e-64 218
MsG0480021738.01.T01 AT3G19830 70.312 64 19 0 322 385 501 564 6.42e-20 92.0
MsG0480021738.01.T01 AT3G19830 60.938 192 40 3 38 229 356 512 1.77e-64 218
MsG0480021738.01.T01 AT3G19830 70.312 64 19 0 322 385 501 564 6.42e-20 92.0
MsG0480021738.01.T01 AT1G50260 58.824 136 27 1 82 217 342 448 8.26e-41 152
MsG0480021738.01.T01 AT1G50260 68.116 69 19 2 317 385 444 509 1.04e-18 88.6

Find 77 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGCCCCTGGAATGCCAATT+TGG 0.250515 4:-63726557 MsG0480021738.01.T01:CDS
ACGGCCAAACAAAATTGTTT+TGG 0.302867 4:-63727530 MsG0480021738.01.T01:CDS
TGATTCCAAATTGGCATTCC+AGG 0.305676 4:+63726552 None:intergenic
GGATTTGCAGATTTGACTAT+TGG 0.318186 4:-63726379 MsG0480021738.01.T01:CDS
ATTTCACATTATTTCCTAAC+TGG 0.319697 4:+63726709 None:intergenic
TGGGAAGGGTGCAGGGGTTT+CGG 0.322125 4:-63725623 MsG0480021738.01.T01:intron
GAGTGTATAGGGCATGGTTT+TGG 0.322949 4:-63725831 MsG0480021738.01.T01:intron
AACAACACCTGATTCCAAAT+TGG 0.330831 4:+63726543 None:intergenic
GATGTTCATGGGAAGGGTGC+AGG 0.350361 4:-63725631 MsG0480021738.01.T01:CDS
GCCAGAAAATATTCTACCTT+TGG 0.350599 4:+63725723 None:intergenic
CAATTCTGGTGAGGCTCATT+CGG 0.355063 4:+63725963 None:intergenic
CCAGAAAATATTCTACCTTT+GGG 0.357191 4:+63725724 None:intergenic
TCATCTAAGATCACCAATTC+TGG 0.360200 4:+63725949 None:intergenic
TAAATCTACTGTAAGAAGTT+TGG 0.378740 4:+63727567 None:intergenic
ATCCCAGAGCGTCCTTAACT+TGG 0.393432 4:+63726398 None:intergenic
CGAATGAGCCTCACCAGAAT+TGG 0.402110 4:-63725962 MsG0480021738.01.T01:intron
TCGGGTTGGAAACAAGCATA+TGG 0.412541 4:+63726440 None:intergenic
GTCGTTGATGGTGTTAAATC+TGG 0.415146 4:-63727463 MsG0480021738.01.T01:CDS
TGGAATGCCAATTTGGAATC+AGG 0.417628 4:-63726550 MsG0480021738.01.T01:intron
ACTACTGTCATTGGCGCCCC+TGG 0.419613 4:-63726570 MsG0480021738.01.T01:CDS
TGTAATGAAGGGGTCCTAGT+AGG 0.433643 4:-63725664 MsG0480021738.01.T01:CDS
CCATATGTCATTCTTAGCCT+TGG 0.439251 4:-63726627 MsG0480021738.01.T01:CDS
ACTGTTGGTCCTGTCGTTGA+TGG 0.440638 4:-63727475 MsG0480021738.01.T01:CDS
TATCCAAGTTAAGGACGCTC+TGG 0.445536 4:-63726401 MsG0480021738.01.T01:CDS
GAAACTTACATCGAGAGAAC+TGG 0.447259 4:+63727953 None:intergenic
GACAGAATTGTTGCTTTACA+AGG 0.450629 4:-63724993 MsG0480021738.01.T01:CDS
ATGTTCATGGGAAGGGTGCA+GGG 0.453715 4:-63725630 MsG0480021738.01.T01:CDS
GTCTGAAACATCTATATCAA+TGG 0.453813 4:+63724866 None:intergenic
AACAGTCAAACTACTGTCAT+TGG 0.458505 4:-63726579 MsG0480021738.01.T01:CDS
CAGCTTTCAATGTGTAATGA+AGG 0.464038 4:-63725676 MsG0480021738.01.T01:CDS
ACATATGGATCTGTCTTGCC+TGG 0.466754 4:+63726642 None:intergenic
GAAGTTAAGTATCCAAGTTA+AGG 0.473337 4:-63726410 MsG0480021738.01.T01:CDS
GCAGATTTGACTATTGGTAC+TGG 0.474231 4:-63726373 MsG0480021738.01.T01:CDS
TATTCACAAGAATACCAGTT+AGG 0.492494 4:-63726723 MsG0480021738.01.T01:CDS
CCAAGGCTAAGAATGACATA+TGG 0.496475 4:+63726627 None:intergenic
GATTCCAAATTGGCATTCCA+GGG 0.496914 4:+63726553 None:intergenic
TTTGGCCGTACAAATAGTCT+AGG 0.514653 4:+63727544 None:intergenic
CAGAATTGTTGCTTTACAAG+GGG 0.518230 4:-63724991 MsG0480021738.01.T01:CDS
ACGACAGGACCAACAGTGAC+GGG 0.535880 4:+63727481 None:intergenic
TGTTCATGGGAAGGGTGCAG+GGG 0.535954 4:-63725629 MsG0480021738.01.T01:CDS
ATTTACCTAGACTATTTGTA+CGG 0.536468 4:-63727549 MsG0480021738.01.T01:CDS
GCTAACATATAAAGCATATG+TGG 0.540309 4:-63724922 MsG0480021738.01.T01:CDS
TAAAGCATATGTGGAGGATG+AGG 0.544623 4:-63724913 MsG0480021738.01.T01:CDS
TGAACTCTCCGTGACTCTTG+TGG 0.547422 4:-63727407 MsG0480021738.01.T01:CDS
TTCGAGAAGGGAAAAGCTGT+TGG 0.550160 4:-63727505 MsG0480021738.01.T01:CDS
GTAATGAAGGGGTCCTAGTA+GGG 0.552426 4:-63725663 MsG0480021738.01.T01:CDS
AAAGCAACAATTCTGTCTGT+TGG 0.552705 4:+63724999 None:intergenic
GCTGTTGGTCCCGTCACTGT+TGG 0.556112 4:-63727490 MsG0480021738.01.T01:CDS
TTCTTTCAGCGACTAAGCAA+AGG 0.558049 4:+63725770 None:intergenic
ATCCAAGTTAAGGACGCTCT+GGG 0.566547 4:-63726400 MsG0480021738.01.T01:CDS
AGCTTTCAATGTGTAATGAA+GGG 0.567363 4:-63725675 MsG0480021738.01.T01:CDS
AACGACAGGACCAACAGTGA+CGG 0.567745 4:+63727480 None:intergenic
AAAGTTGGATGTTCATGGGA+AGG 0.577255 4:-63725638 MsG0480021738.01.T01:CDS
CGTCTGGGGAGATATTGCTT+AGG 0.577276 4:-63724944 MsG0480021738.01.T01:CDS
TGCGTATGGTTTGATCTCCA+AGG 0.577385 4:+63726610 None:intergenic
CTGGCGTGTGCTGGGAACAA+TGG 0.582063 4:-63725705 MsG0480021738.01.T01:CDS
CATCAGCAACAGAGGAGGAA+GGG 0.585165 4:+63726003 None:intergenic
ACAGAATTGTTGCTTTACAA+GGG 0.587625 4:-63724992 MsG0480021738.01.T01:CDS
TCATCAGCAACAGAGGAGGA+AGG 0.587724 4:+63726002 None:intergenic
GAAGGAAACATGGATTCTGT+TGG 0.589437 4:-63727430 MsG0480021738.01.T01:CDS
AGAAATGCAAGAAGGAAACA+TGG 0.598978 4:-63727440 MsG0480021738.01.T01:CDS
GATTTAACACCATCAACGAC+AGG 0.604084 4:+63727466 None:intergenic
TCGAGCATCCACAAGAGTCA+CGG 0.610176 4:+63727399 None:intergenic
TTTACGAAAGGGCTCGTCTG+GGG 0.616659 4:-63724958 MsG0480021738.01.T01:CDS
AAGTTGGATGTTCATGGGAA+GGG 0.624159 4:-63725637 MsG0480021738.01.T01:CDS
AAATCTGGAGAAATGCAAGA+AGG 0.625821 4:-63727448 MsG0480021738.01.T01:CDS
GTTCAATTCATCAGCAACAG+AGG 0.626851 4:+63725995 None:intergenic
GCTTTCAATGTGTAATGAAG+GGG 0.631094 4:-63725674 MsG0480021738.01.T01:CDS
TTTGACTATTGGTACTGGAG+AGG 0.632633 4:-63726368 MsG0480021738.01.T01:CDS
AACATATAAAGCATATGTGG+AGG 0.636889 4:-63724919 MsG0480021738.01.T01:CDS
TAAGATCACCAATTCTGGTG+AGG 0.644887 4:+63725954 None:intergenic
CAATTCATCAGCAACAGAGG+AGG 0.653252 4:+63725998 None:intergenic
ATTCCAAATTGGCATTCCAG+GGG 0.656517 4:+63726554 None:intergenic
CTTAGTCGCTGAAAGAACTG+TGG 0.664598 4:-63725764 MsG0480021738.01.T01:CDS
AGAATTGTTGCTTTACAAGG+GGG 0.665731 4:-63724990 MsG0480021738.01.T01:CDS
GGAAGATGACAAGACTGAGG+AGG 0.675289 4:-63724892 MsG0480021738.01.T01:CDS
TGAGGAAGATGACAAGACTG+AGG 0.700576 4:-63724895 MsG0480021738.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAATATAAGACAAAA+TGG + Chr4:63726156-63726175 None:intergenic 15.0%
!! AAACAAATATAAGACAAAAT+GGG + Chr4:63726155-63726174 None:intergenic 15.0%
!! AACACAATAAATATTTAATC+TGG + Chr4:63725173-63725192 None:intergenic 15.0%
!! GCTTAAATTATTTAATCTTA+CGG - Chr4:63727018-63727037 MsG0480021738.01.T01:intron 15.0%
!! TTGTAAATGCATATTTAAAA+TGG - Chr4:63725045-63725064 MsG0480021738.01.T01:intron 15.0%
!!! AAATATGTATTTTAAATGCA+AGG + Chr4:63724867-63724886 None:intergenic 15.0%
!!! ATAGATTTTACCAAAAAATA+AGG + Chr4:63725551-63725570 None:intergenic 15.0%
!!! ATATCTATAACATGTATTTT+TGG - Chr4:63726780-63726799 MsG0480021738.01.T01:intron 15.0%
!!! TTGCTGTTTTTTTTTTTTTT+TGG - Chr4:63725596-63725615 MsG0480021738.01.T01:intron 15.0%
!!! TTTTATCCTCTAATATTATT+TGG - Chr4:63725726-63725745 MsG0480021738.01.T01:CDS 15.0%
!! AACAAATATAAGACAAAATG+GGG + Chr4:63726154-63726173 None:intergenic 20.0%
!! ATAGCAAATAAAAAATTGAG+TGG + Chr4:63726252-63726271 None:intergenic 20.0%
!! CGAGAAAATATTTGAAAAAT+TGG + Chr4:63726806-63726825 None:intergenic 20.0%
!! TAAATTATTTAATCTTACGG+AGG - Chr4:63727021-63727040 MsG0480021738.01.T01:intron 20.0%
!! TAGCAAATAAAAAATTGAGT+GGG + Chr4:63726251-63726270 None:intergenic 20.0%
!! TTTATTGTGTTAATCGTAAA+TGG - Chr4:63725182-63725201 MsG0480021738.01.T01:intron 20.0%
!!! AAATCCAAAACAATTTTGTT+TGG + Chr4:63725395-63725414 None:intergenic 20.0%
!!! TAATGATTTGTTCGTTTTAT+TGG - Chr4:63726080-63726099 MsG0480021738.01.T01:intron 20.0%
!!! TATATTAGTATCGATTAACA+TGG - Chr4:63725775-63725794 MsG0480021738.01.T01:CDS 20.0%
!!! TCTTATTTGATAATGTTTTC+AGG - Chr4:63727867-63727886 MsG0480021738.01.T01:intron 20.0%
!!! TTTAGTACTTTTATGTTATG+TGG - Chr4:63725679-63725698 MsG0480021738.01.T01:CDS 20.0%
!!! TTTTTATTTGCTATTTTTCC+AGG - Chr4:63726258-63726277 MsG0480021738.01.T01:intron 20.0%
! AAAACACACAATATTCTGAA+TGG - Chr4:63727649-63727668 MsG0480021738.01.T01:intron 25.0%
! AACACAAATTTAATGGCAAA+AGG + Chr4:63727421-63727440 None:intergenic 25.0%
! AAGACTATACTACATCTAAA+TGG + Chr4:63727590-63727609 None:intergenic 25.0%
! AGCAAATAAAAAATTGAGTG+GGG + Chr4:63726250-63726269 None:intergenic 25.0%
! AGTATAAACAAAGATGAATC+AGG + Chr4:63726410-63726429 None:intergenic 25.0%
! ATGAATATTCTCTATAGAAG+AGG - Chr4:63725970-63725989 MsG0480021738.01.T01:CDS 25.0%
! ATTTACCTAGACTATTTGTA+CGG - Chr4:63725369-63725388 MsG0480021738.01.T01:intron 25.0%
! ATTTCACATTATTTCCTAAC+TGG + Chr4:63726212-63726231 None:intergenic 25.0%
! CTAACTGTATGTAATTATTC+TGG - Chr4:63725306-63725325 MsG0480021738.01.T01:intron 25.0%
! TAAATCTACTGTAAGAAGTT+TGG + Chr4:63725354-63725373 None:intergenic 25.0%
! TGGATTATTTGTAGTAGATT+GGG - Chr4:63725616-63725635 MsG0480021738.01.T01:intron 25.0%
! TGTGAACCAAATAATATTAG+AGG + Chr4:63725735-63725754 None:intergenic 25.0%
! TGTTTGCAATTGATTAAGAA+TGG - Chr4:63727819-63727838 MsG0480021738.01.T01:intron 25.0%
! TTGGATTATTTGTAGTAGAT+TGG - Chr4:63725615-63725634 MsG0480021738.01.T01:intron 25.0%
! TTTAAAAATAAGAAGCCCAA+AGG - Chr4:63727179-63727198 MsG0480021738.01.T01:intron 25.0%
!! AATATTGTGTGTTTTGCAAT+GGG + Chr4:63727644-63727663 None:intergenic 25.0%
!! ATATTTTCTCGTTAAAAGTC+TGG - Chr4:63726815-63726834 MsG0480021738.01.T01:intron 25.0%
!! TCATAACTAAAACGTAGTTT+TGG - Chr4:63726111-63726130 MsG0480021738.01.T01:intron 25.0%
!! TTTCTTCTTTTTGCATCATT+GGG - Chr4:63724928-63724947 MsG0480021738.01.T01:CDS 25.0%
!!! ATAATGTTTTCAGGTTGATT+TGG - Chr4:63727876-63727895 MsG0480021738.01.T01:intron 25.0%
!!! ATACTTAACTTCTGCTTTTT+CGG + Chr4:63726500-63726519 None:intergenic 25.0%
!!! GTTATTAGTATCGATTAACA+TGG - Chr4:63725837-63725856 MsG0480021738.01.T01:intron 25.0%
!!! TAATGTTTTCAGGTTGATTT+GGG - Chr4:63727877-63727896 MsG0480021738.01.T01:intron 25.0%
!!! TTTGGGCTTCTTATTTTTAA+AGG + Chr4:63727180-63727199 None:intergenic 25.0%
AAACGAAGAGAAATTACTCA+AGG + Chr4:63726058-63726077 None:intergenic 30.0%
AACATATAAAGCATATGTGG+AGG - Chr4:63727999-63728018 MsG0480021738.01.T01:CDS 30.0%
AATGGCAAAAGGATAAAAGA+AGG + Chr4:63727410-63727429 None:intergenic 30.0%
ACAGAGGAACACAAATTTAA+TGG + Chr4:63727428-63727447 None:intergenic 30.0%
ACTAATAAGTAGAGATCACA+AGG + Chr4:63726629-63726648 None:intergenic 30.0%
AGCTTTCAATGTGTAATGAA+GGG - Chr4:63727243-63727262 MsG0480021738.01.T01:intron 30.0%
ATTAGAAAGTAACTGCACTT+TGG - Chr4:63725133-63725152 MsG0480021738.01.T01:intron 30.0%
ATTCTTGTGAATACTGAAAC+TGG + Chr4:63726188-63726207 None:intergenic 30.0%
ATTTGTGTTCCTCTGTTATA+AGG - Chr4:63727432-63727451 MsG0480021738.01.T01:CDS 30.0%
CCAGAAAATATTCTACCTTT+GGG + Chr4:63727197-63727216 None:intergenic 30.0%
GAAAAGTTATGCAGCAAAAA+GGG + Chr4:63727467-63727486 None:intergenic 30.0%
GAAGTTAAGTATCCAAGTTA+AGG - Chr4:63726508-63726527 MsG0480021738.01.T01:intron 30.0%
GCTAACATATAAAGCATATG+TGG - Chr4:63727996-63728015 MsG0480021738.01.T01:CDS 30.0%
GTCAAAAACCTACAAGAAAA+AGG + Chr4:63725332-63725351 None:intergenic 30.0%
TAAGCAAAGATGTGCTTATT+AGG - Chr4:63726888-63726907 MsG0480021738.01.T01:intron 30.0%
TAATTACATACAGTTAGTGC+AGG + Chr4:63725303-63725322 None:intergenic 30.0%
TATTCACAAGAATACCAGTT+AGG - Chr4:63726195-63726214 MsG0480021738.01.T01:intron 30.0%
TGACTCATTATTATTGCATG+TGG - Chr4:63726854-63726873 MsG0480021738.01.T01:intron 30.0%
TGGCATGAAAATAGTTAACA+TGG + Chr4:63727570-63727589 None:intergenic 30.0%
TGTGAATACAACGGAAAATA+TGG + Chr4:63726437-63726456 None:intergenic 30.0%
! ATATTGTGTGTTTTGCAATG+GGG + Chr4:63727643-63727662 None:intergenic 30.0%
! GAATATTGTGTGTTTTGCAA+TGG + Chr4:63727645-63727664 None:intergenic 30.0%
! GTCTGAAACATCTATATCAA+TGG + Chr4:63728055-63728074 None:intergenic 30.0%
! GTTATGGATTATCTCTAGAT+TGG - Chr4:63725257-63725276 MsG0480021738.01.T01:intron 30.0%
! GTTTCTTCTTTTTGCATCAT+TGG - Chr4:63724927-63724946 MsG0480021738.01.T01:CDS 30.0%
!! ACAGAATTGTTGCTTTACAA+GGG - Chr4:63727926-63727945 MsG0480021738.01.T01:intron 30.0%
!! GTGCAATACCAAAAGTATTT+GGG - Chr4:63726585-63726604 MsG0480021738.01.T01:CDS 30.0%
!! TGTGCAATACCAAAAGTATT+TGG - Chr4:63726584-63726603 MsG0480021738.01.T01:CDS 30.0%
!!! AATTGTTTTGGATTTCGAGA+AGG - Chr4:63725400-63725419 MsG0480021738.01.T01:intron 30.0%
!!! ATTGTTTTGGATTTCGAGAA+GGG - Chr4:63725401-63725420 MsG0480021738.01.T01:intron 30.0%
!!! TACCAAAAGTATTTGGGTTT+TGG - Chr4:63726591-63726610 MsG0480021738.01.T01:CDS 30.0%
!!! TACTTAACTTCTGCTTTTTC+GGG + Chr4:63726499-63726518 None:intergenic 30.0%
!!! TCGAAAGCTTCCTTATTTTT+TGG - Chr4:63725538-63725557 MsG0480021738.01.T01:intron 30.0%
AAAGCAACAATTCTGTCTGT+TGG + Chr4:63727922-63727941 None:intergenic 35.0%
AAATCTGGAGAAATGCAAGA+AGG - Chr4:63725470-63725489 MsG0480021738.01.T01:intron 35.0%
AACAACACCTGATTCCAAAT+TGG + Chr4:63726378-63726397 None:intergenic 35.0%
AACAGTCAAACTACTGTCAT+TGG - Chr4:63726339-63726358 MsG0480021738.01.T01:intron 35.0%
AACATCACTACTTTGATGCA+AGG + Chr4:63727743-63727762 None:intergenic 35.0%
ACATCACTACTTTGATGCAA+GGG + Chr4:63727742-63727761 None:intergenic 35.0%
AGAAATGCAAGAAGGAAACA+TGG - Chr4:63725478-63725497 MsG0480021738.01.T01:intron 35.0%
CAAATAAGAAACATACACCC+AGG + Chr4:63727856-63727875 None:intergenic 35.0%
CAGCTTTCAATGTGTAATGA+AGG - Chr4:63727242-63727261 MsG0480021738.01.T01:intron 35.0%
CATAACGATGAAGAGAAACA+AGG + Chr4:63725243-63725262 None:intergenic 35.0%
CGAAAAGTTATGCAGCAAAA+AGG + Chr4:63727468-63727487 None:intergenic 35.0%
GCCAGAAAATATTCTACCTT+TGG + Chr4:63727198-63727217 None:intergenic 35.0%
GCTAGAACATGTGAATACAA+CGG + Chr4:63726446-63726465 None:intergenic 35.0%
GCTTTCAATGTGTAATGAAG+GGG - Chr4:63727244-63727263 MsG0480021738.01.T01:intron 35.0%
GGAAAAAGTTGGATGTTCAT+GGG - Chr4:63727276-63727295 MsG0480021738.01.T01:intron 35.0%
GGATTTGCAGATTTGACTAT+TGG - Chr4:63726539-63726558 MsG0480021738.01.T01:intron 35.0%
GTTATGTGGACAATGTTATG+TGG - Chr4:63725693-63725712 MsG0480021738.01.T01:CDS 35.0%
TAAAAATCCCAGCACGTTAA+AGG + Chr4:63727543-63727562 None:intergenic 35.0%
TGTCAACTACTCTCTTTGAT+GGG + Chr4:63727505-63727524 None:intergenic 35.0%
TTGTCAACTACTCTCTTTGA+TGG + Chr4:63727506-63727525 None:intergenic 35.0%
! ACGGCCAAACAAAATTGTTT+TGG - Chr4:63725388-63725407 MsG0480021738.01.T01:intron 35.0%
! CACCAAAACCCAAATACTTT+TGG + Chr4:63726596-63726615 None:intergenic 35.0%
! CCCAAAGGTAGAATATTTTC+TGG - Chr4:63727194-63727213 MsG0480021738.01.T01:intron 35.0%
! CTTGTTTCTCTTCATCGTTA+TGG - Chr4:63725241-63725260 MsG0480021738.01.T01:intron 35.0%
! TCATCTAAGATCACCAATTC+TGG + Chr4:63726972-63726991 None:intergenic 35.0%
!! AGAATTGTTGCTTTACAAGG+GGG - Chr4:63727928-63727947 MsG0480021738.01.T01:intron 35.0%
!! CAGAATTGTTGCTTTACAAG+GGG - Chr4:63727927-63727946 MsG0480021738.01.T01:intron 35.0%
!! GACAGAATTGTTGCTTTACA+AGG - Chr4:63727925-63727944 MsG0480021738.01.T01:intron 35.0%
!! TATTCTGGCCTTTTTCTTGT+AGG - Chr4:63725321-63725340 MsG0480021738.01.T01:intron 35.0%
!! TGAATGCTTTGGAGTGTATA+GGG - Chr4:63727076-63727095 MsG0480021738.01.T01:intron 35.0%
!!! AGAATGGAGCTATTTTTTCC+TGG - Chr4:63727835-63727854 MsG0480021738.01.T01:intron 35.0%
!!! GAATGGAGCTATTTTTTCCT+GGG - Chr4:63727836-63727855 MsG0480021738.01.T01:intron 35.0%
AAAGTTGGATGTTCATGGGA+AGG - Chr4:63727280-63727299 MsG0480021738.01.T01:intron 40.0%
AAGTTGGATGTTCATGGGAA+GGG - Chr4:63727281-63727300 MsG0480021738.01.T01:intron 40.0%
ACTACTTTGATGCAAGGGAT+TGG + Chr4:63727737-63727756 None:intergenic 40.0%
AGAGGCAGAAGAAAGGAAAA+AGG - Chr4:63725988-63726007 MsG0480021738.01.T01:CDS 40.0%
AGCTCAACTCCTTATAACAG+AGG + Chr4:63727444-63727463 None:intergenic 40.0%
AGGCAGAAGAAAGGAAAAAG+GGG - Chr4:63725990-63726009 MsG0480021738.01.T01:CDS 40.0%
ATTCCAAATTGGCATTCCAG+GGG + Chr4:63726367-63726386 None:intergenic 40.0%
CCAAGGCTAAGAATGACATA+TGG + Chr4:63726294-63726313 None:intergenic 40.0%
CCATATGTCATTCTTAGCCT+TGG - Chr4:63726291-63726310 MsG0480021738.01.T01:intron 40.0%
CTACTTTGATGCAAGGGATT+GGG + Chr4:63727736-63727755 None:intergenic 40.0%
CTATAGAAGAGGCAGAAGAA+AGG - Chr4:63725981-63726000 MsG0480021738.01.T01:CDS 40.0%
CTTAGTGTCTTGTCTGTCTA+TGG - Chr4:63726724-63726743 MsG0480021738.01.T01:CDS 40.0%
GAAACTTACATCGAGAGAAC+TGG + Chr4:63724968-63724987 None:intergenic 40.0%
GAAGGAAACATGGATTCTGT+TGG - Chr4:63725488-63725507 MsG0480021738.01.T01:intron 40.0%
GAGGCAGAAGAAAGGAAAAA+GGG - Chr4:63725989-63726008 MsG0480021738.01.T01:CDS 40.0%
GATTAACATGGTAGACTCCA+AGG - Chr4:63725787-63725806 MsG0480021738.01.T01:CDS 40.0%
GATTCCAAATTGGCATTCCA+GGG + Chr4:63726368-63726387 None:intergenic 40.0%
GATTTAACACCATCAACGAC+AGG + Chr4:63725455-63725474 None:intergenic 40.0%
GCAATTAGCATGCAGCATTA+TGG + Chr4:63726751-63726770 None:intergenic 40.0%
GCACATCTTTGCTTAAGACA+AGG + Chr4:63726883-63726902 None:intergenic 40.0%
GCAGATTTGACTATTGGTAC+TGG - Chr4:63726545-63726564 MsG0480021738.01.T01:intron 40.0%
GGGAAAAAGTTGGATGTTCA+TGG - Chr4:63727275-63727294 MsG0480021738.01.T01:intron 40.0%
GTTCAATTCATCAGCAACAG+AGG + Chr4:63726926-63726945 None:intergenic 40.0%
TAAAGCATATGTGGAGGATG+AGG - Chr4:63728005-63728024 MsG0480021738.01.T01:CDS 40.0%
TAAGATCACCAATTCTGGTG+AGG + Chr4:63726967-63726986 None:intergenic 40.0%
TGATTCCAAATTGGCATTCC+AGG + Chr4:63726369-63726388 None:intergenic 40.0%
TTCACATGTTCTAGCACTAC+AGG - Chr4:63726451-63726470 MsG0480021738.01.T01:intron 40.0%
TTCTTTCAGCGACTAAGCAA+AGG + Chr4:63727151-63727170 None:intergenic 40.0%
TTTGGCCGTACAAATAGTCT+AGG + Chr4:63725377-63725396 None:intergenic 40.0%
! AATACCACACTTTTCGACCA+CGG - Chr4:63727614-63727633 MsG0480021738.01.T01:intron 40.0%
! ACTGTTCTTTTTGCTGCGTA+TGG + Chr4:63726325-63726344 None:intergenic 40.0%
! GATTATCTCTAGATTGGCTG+TGG - Chr4:63725263-63725282 MsG0480021738.01.T01:intron 40.0%
! TCTAGATTGGCTGTGGAAAT+TGG - Chr4:63725270-63725289 MsG0480021738.01.T01:intron 40.0%
! TCTTTTTGCATCATTGGGCA+TGG - Chr4:63724933-63724952 MsG0480021738.01.T01:CDS 40.0%
! TGGAATGCCAATTTGGAATC+AGG - Chr4:63726368-63726387 MsG0480021738.01.T01:CDS 40.0%
! TTTGACTATTGGTACTGGAG+AGG - Chr4:63726550-63726569 MsG0480021738.01.T01:intron 40.0%
!! AATATTTTCTGGCGTGTGCT+GGG - Chr4:63727205-63727224 MsG0480021738.01.T01:intron 40.0%
!! AGAGAGTTTTGTGGTGATGT+TGG - Chr4:63727335-63727354 MsG0480021738.01.T01:intron 40.0%
!! AGTTTTGTGGTGATGTTGGT+GGG - Chr4:63727339-63727358 MsG0480021738.01.T01:intron 40.0%
!! CATCCAACTTTTTCCCTACT+AGG + Chr4:63727271-63727290 None:intergenic 40.0%
!! CTGAATGCTTTGGAGTGTAT+AGG - Chr4:63727075-63727094 MsG0480021738.01.T01:intron 40.0%
!! GTCGTTGATGGTGTTAAATC+TGG - Chr4:63725455-63725474 MsG0480021738.01.T01:intron 40.0%
!!! GGTTGGGGTTTTTTACGAAA+GGG - Chr4:63727949-63727968 MsG0480021738.01.T01:intron 40.0%
!!! TAACTTCTGCTTTTTCGGGT+TGG + Chr4:63726495-63726514 None:intergenic 40.0%
AAATCCCAGCACGTTAAAGG+AGG + Chr4:63727540-63727559 None:intergenic 45.0%
ACATATGGATCTGTCTTGCC+TGG + Chr4:63726279-63726298 None:intergenic 45.0%
ATCCAAGTTAAGGACGCTCT+GGG - Chr4:63726518-63726537 MsG0480021738.01.T01:intron 45.0%
CAATTCATCAGCAACAGAGG+AGG + Chr4:63726923-63726942 None:intergenic 45.0%
CAATTCTGGTGAGGCTCATT+CGG + Chr4:63726958-63726977 None:intergenic 45.0%
CATCTGAAGACTGACTACCT+TGG + Chr4:63725807-63725826 None:intergenic 45.0%
CTTAGTCGCTGAAAGAACTG+TGG - Chr4:63727154-63727173 MsG0480021738.01.T01:intron 45.0%
GGTCCTAGTAGGGAAAAAGT+TGG - Chr4:63727265-63727284 MsG0480021738.01.T01:intron 45.0%
GTAATGAAGGGGTCCTAGTA+GGG - Chr4:63727255-63727274 MsG0480021738.01.T01:intron 45.0%
TATCCAAGTTAAGGACGCTC+TGG - Chr4:63726517-63726536 MsG0480021738.01.T01:intron 45.0%
TGAGGAAGATGACAAGACTG+AGG - Chr4:63728023-63728042 MsG0480021738.01.T01:CDS 45.0%
TGCCACTACACTGAATGCTT+TGG - Chr4:63727065-63727084 MsG0480021738.01.T01:intron 45.0%
TGCGTATGGTTTGATCTCCA+AGG + Chr4:63726311-63726330 None:intergenic 45.0%
TGTAATGAAGGGGTCCTAGT+AGG - Chr4:63727254-63727273 MsG0480021738.01.T01:intron 45.0%
TTCGAGAAGGGAAAAGCTGT+TGG - Chr4:63725413-63725432 MsG0480021738.01.T01:intron 45.0%
TTGTCCTCCTTTAACGTGCT+GGG - Chr4:63727533-63727552 MsG0480021738.01.T01:CDS 45.0%
! CTCCAAAGCATTCAGTGTAG+TGG + Chr4:63727070-63727089 None:intergenic 45.0%
! GAGTGTATAGGGCATGGTTT+TGG - Chr4:63727087-63727106 MsG0480021738.01.T01:intron 45.0%
! TTTTACGAAAGGGCTCGTCT+GGG - Chr4:63727959-63727978 MsG0480021738.01.T01:intron 45.0%
! TTTTTACGAAAGGGCTCGTC+TGG - Chr4:63727958-63727977 MsG0480021738.01.T01:intron 45.0%
!! GAATATTTTCTGGCGTGTGC+TGG - Chr4:63727204-63727223 MsG0480021738.01.T01:intron 45.0%
!! GAGTTTTGTGGTGATGTTGG+TGG - Chr4:63727338-63727357 MsG0480021738.01.T01:intron 45.0%
!! TCGGGTTGGAAACAAGCATA+TGG + Chr4:63726481-63726500 None:intergenic 45.0%
!! TGTTGCTTTACAAGGGGGTT+GGG - Chr4:63727933-63727952 MsG0480021738.01.T01:intron 45.0%
!! TTGTTGCTTTACAAGGGGGT+TGG - Chr4:63727932-63727951 MsG0480021738.01.T01:intron 45.0%
!!! GGGTTGGGGTTTTTTACGAA+AGG - Chr4:63727948-63727967 MsG0480021738.01.T01:intron 45.0%
AACGACAGGACCAACAGTGA+CGG + Chr4:63725441-63725460 None:intergenic 50.0%
ACTGTTGGTCCTGTCGTTGA+TGG - Chr4:63725443-63725462 MsG0480021738.01.T01:intron 50.0%
CATCAGCAACAGAGGAGGAA+GGG + Chr4:63726918-63726937 None:intergenic 50.0%
CGAATGAGCCTCACCAGAAT+TGG - Chr4:63726956-63726975 MsG0480021738.01.T01:intron 50.0%
CGTCTGGGGAGATATTGCTT+AGG - Chr4:63727974-63727993 MsG0480021738.01.T01:CDS 50.0%
GGAAGATGACAAGACTGAGG+AGG - Chr4:63728026-63728045 MsG0480021738.01.T01:CDS 50.0%
GGTTTCGGTAAGGGAAGAGA+GGG - Chr4:63727310-63727329 MsG0480021738.01.T01:intron 50.0%
GTTGTCCTCCTTTAACGTGC+TGG - Chr4:63727532-63727551 MsG0480021738.01.T01:CDS 50.0%
GTTTCGGTAAGGGAAGAGAG+GGG - Chr4:63727311-63727330 MsG0480021738.01.T01:intron 50.0%
TCATCAGCAACAGAGGAGGA+AGG + Chr4:63726919-63726938 None:intergenic 50.0%
TCGAGCATCCACAAGAGTCA+CGG + Chr4:63725522-63725541 None:intergenic 50.0%
TGAACTCTCCGTGACTCTTG+TGG - Chr4:63725511-63725530 MsG0480021738.01.T01:intron 50.0%
TTTACGAAAGGGCTCGTCTG+GGG - Chr4:63727960-63727979 MsG0480021738.01.T01:intron 50.0%
! ATCCCAGAGCGTCCTTAACT+TGG + Chr4:63726523-63726542 None:intergenic 50.0%
! ATGTTCATGGGAAGGGTGCA+GGG - Chr4:63727288-63727307 MsG0480021738.01.T01:intron 50.0%
! GAGAGGGGAAGAGAGTTTTG+TGG - Chr4:63727326-63727345 MsG0480021738.01.T01:intron 50.0%
! GTTGCTTTACAAGGGGGTTG+GGG - Chr4:63727934-63727953 MsG0480021738.01.T01:intron 50.0%
!! GCTTTGGAGTGTATAGGGCA+TGG - Chr4:63727081-63727100 MsG0480021738.01.T01:intron 50.0%
ACGACAGGACCAACAGTGAC+GGG + Chr4:63725440-63725459 None:intergenic 55.0%
GGGTTTCGGTAAGGGAAGAG+AGG - Chr4:63727309-63727328 MsG0480021738.01.T01:intron 55.0%
! ACCACACTTTTCGACCACGG+TGG - Chr4:63727617-63727636 MsG0480021738.01.T01:intron 55.0%
! ATGTTGGTGGGCCATTGGCT+TGG - Chr4:63727351-63727370 MsG0480021738.01.T01:intron 55.0%
! GATGTTCATGGGAAGGGTGC+AGG - Chr4:63727287-63727306 MsG0480021738.01.T01:intron 55.0%
! TGGTGATGTTGGTGGGCCAT+TGG - Chr4:63727346-63727365 MsG0480021738.01.T01:intron 55.0%
! TGTTCATGGGAAGGGTGCAG+GGG - Chr4:63727289-63727308 MsG0480021738.01.T01:intron 55.0%
!! TGTTGGTGGGCCATTGGCTT+GGG - Chr4:63727352-63727371 MsG0480021738.01.T01:intron 55.0%
ACTACTGTCATTGGCGCCCC+TGG - Chr4:63726348-63726367 MsG0480021738.01.T01:CDS 60.0%
AGGGTGCAGGGGTTTCGGTA+AGG - Chr4:63727300-63727319 MsG0480021738.01.T01:intron 60.0%
CTGGCGTGTGCTGGGAACAA+TGG - Chr4:63727213-63727232 MsG0480021738.01.T01:intron 60.0%
GCAATCTCCGCCCAAGCCAA+TGG + Chr4:63727365-63727384 None:intergenic 60.0%
GCCACCGTGGTCGAAAAGTG+TGG + Chr4:63727621-63727640 None:intergenic 60.0%
GCGCCCCTGGAATGCCAATT+TGG - Chr4:63726361-63726380 MsG0480021738.01.T01:CDS 60.0%
GCTGTTGGTCCCGTCACTGT+TGG - Chr4:63725428-63725447 MsG0480021738.01.T01:intron 60.0%
GGGTGCAGGGGTTTCGGTAA+GGG - Chr4:63727301-63727320 MsG0480021738.01.T01:intron 60.0%
! GTTTTGCAATGGGGCCACCG+TGG + Chr4:63727634-63727653 None:intergenic 60.0%
! TGGGAAGGGTGCAGGGGTTT+CGG - Chr4:63727295-63727314 MsG0480021738.01.T01:intron 60.0%
!! TGGTGGGCCATTGGCTTGGG+CGG - Chr4:63727355-63727374 MsG0480021738.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 63724848 63728092 63724848 ID=MsG0480021738.01;Name=MsG0480021738.01
Chr4 mRNA 63724848 63728092 63724848 ID=MsG0480021738.01.T01;Parent=MsG0480021738.01;Name=MsG0480021738.01.T01;_AED=0.50;_eAED=0.62;_QI=0|0.5|0.44|1|0.5|0.44|9|0|388
Chr4 exon 63727962 63728092 63727962 ID=MsG0480021738.01.T01:exon:3518;Parent=MsG0480021738.01.T01
Chr4 exon 63727381 63727597 63727381 ID=MsG0480021738.01.T01:exon:3519;Parent=MsG0480021738.01.T01
Chr4 exon 63726551 63726751 63726551 ID=MsG0480021738.01.T01:exon:3520;Parent=MsG0480021738.01.T01
Chr4 exon 63726342 63726467 63726342 ID=MsG0480021738.01.T01:exon:3521;Parent=MsG0480021738.01.T01
Chr4 exon 63725963 63726030 63725963 ID=MsG0480021738.01.T01:exon:3522;Parent=MsG0480021738.01.T01
Chr4 exon 63725624 63725843 63725624 ID=MsG0480021738.01.T01:exon:3523;Parent=MsG0480021738.01.T01
Chr4 exon 63724848 63725051 63724848 ID=MsG0480021738.01.T01:exon:3524;Parent=MsG0480021738.01.T01
Chr4 CDS 63727962 63728092 63727962 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63727381 63727597 63727381 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63726551 63726751 63726551 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63726342 63726467 63726342 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63725963 63726030 63725963 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63725624 63725843 63725624 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Chr4 CDS 63724848 63725051 63724848 ID=MsG0480021738.01.T01:cds;Parent=MsG0480021738.01.T01
Gene Sequence

>MsG0480021738.01.T01

ATGTTACATATCATTTCCTTGCATTTAAAATACATATTTTTGAAGAATATCGAATTCATTGTTGCTGACATTTCTGTTTGTTTCTTCTTTTTGCATCATTGGGCATGGTCAGCAATTCCAGTTCTCTCGATGTTTTTGACCAAACTTCTTACAGTAGATTTACCTAGACTATTTGTACGGCCAAACAAAATTGTTTTGGATTTCGAGAAGGGAAAAGCTGTTGGTCCCGTCACTGTTGGTCCTGTCGTTGATGGTGTTAAATCTGGAGAAATGCAAGAAGGAAACATGGATTCTGTTGGTGAACTCTCCGTGACTCTTGTGGATGCTCGAAAGCTTCCTTATTTTTTGTTTCAGTATTCACAAGAATACCAGTTAGGAAATAATGTGAAATATGTTGCATCATCTGCCCCACTCAATTTTTTATTTGCTATTTTTCCAGGCAAGACAGATCCATATGTCATTCTTAGCCTTGGAGATCAAACCATACGCAGCAAAAAGAACAGTCAAACTACTGTCATTGGCGCCCCTGGAATGCCAATTTGGAATCAGGATTTCCATATGCTTGTTTCCAACCCGAAAAAGCAGAAGTTAAGTATCCAAGTTAAGGACGCTCTGGGATTTGCAGATTTGACTATTGGTACTGGAGAGGTATATATTATGTGTGCAATACCAAAAGTTCTCCCTTCCTCCTCTGTTGCTGATGAATTGAACAGAGTTAAGCCGAATGAGCCTCACCAGAATTGGGCATGGTTTTGGACTGTATCGAGTATGAATATGACTTCTTCTAATTCTTTCCTTTGCTTAGTCGCTGAAAGAACTGTGGCCTTTAAAAATAAGAAGCCCAAAGGTAGAATATTTTCTGGCGTGTGCTGGGAACAATGGTGTAGACAGCTTTCAATGTGTAATGAAGGGGTCCTAGTAGGGAAAAAGTTGGATGTTCATGGGAAGGGTGCAGGGGTTTCGGTTGATTTGGGATCTCTTCAAGACACTGTACCAACAGACAGAATTGTTGCTTTACAAGGGGGTTGGGGTTTTTTACGAAAGGGCTCGTCTGGGGAGATATTGCTTAGGCTAACATATAAAGCATATGTGGAGGATGAGGAAGATGACAAGACTGAGGAGGATTCCATTGATATAGATGTTTCAGACGATGATTGTCTGATATAG
Protein sequence

>MsG0480021738.01.T01

MLHIISLHLKYIFLKNIEFIVADISVCFFFLHHWAWSAIPVLSMFLTKLLTVDLPRLFVRPNKIVLDFEKGKAVGPVTVGPVVDGVKSGEMQEGNMDSVGELSVTLVDARKLPYFLFQYSQEYQLGNNVKYVASSAPLNFLFAIFPGKTDPYVILSLGDQTIRSKKNSQTTVIGAPGMPIWNQDFHMLVSNPKKQKLSIQVKDALGFADLTIGTGEVYIMCAIPKVLPSSSVADELNRVKPNEPHQNWAWFWTVSSMNMTSSNSFLCLVAERTVAFKNKKPKGRIFSGVCWEQWCRQLSMCNEGVLVGKKLDVHGKGAGVSVDLGSLQDTVPTDRIVALQGGWGFLRKGSSGEILLRLTYKAYVEDEEDDKTEEDSIDIDVSDDDCLI*