Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021737.01.T01 | XP_024636780.1 | 92.405 | 79 | 6 | 0 | 2 | 80 | 182 | 260 | 1.73E-41 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021737.01.T01 | A0A396IH69 | 92.405 | 79 | 6 | 0 | 2 | 80 | 231 | 309 | 6.43e-41 | 146 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048016.01 | MsG0480021737.01 | 0.804932 | 1.703409e-49 | 8.896127e-47 |
| MsG0180000509.01 | MsG0480021737.01 | 0.815358 | 9.632998e-52 | 6.626882e-49 |
| MsG0180001130.01 | MsG0480021737.01 | 0.832062 | 1.171358e-55 | 1.294809e-52 |
| MsG0180006104.01 | MsG0480021737.01 | 0.808738 | 2.672819e-50 | 1.540138e-47 |
| MsG0380013471.01 | MsG0480021737.01 | 0.813062 | 3.095522e-51 | 2.000870e-48 |
| MsG0380014214.01 | MsG0480021737.01 | 0.828869 | 7.071126e-55 | 7.116342e-52 |
| MsG0480021488.01 | MsG0480021737.01 | 0.912061 | 2.995654e-83 | 6.610172e-79 |
| MsG0480021737.01 | MsG0480021738.01 | 0.850969 | 1.203495e-60 | 2.398964e-57 |
| MsG0480021737.01 | MsG0480021739.01 | 0.915477 | 5.620647e-85 | 1.470830e-80 |
| MsG0480021737.01 | MsG0480022155.01 | 0.806631 | 7.488671e-50 | 4.084482e-47 |
| MsG0480021737.01 | MsG0480022239.01 | 0.805333 | 1.404383e-49 | 7.409712e-47 |
| MsG0480021737.01 | MsG0480023641.01 | 0.845646 | 3.566210e-59 | 5.983532e-56 |
| MsG0480021737.01 | MsG0580028925.01 | 0.800415 | 1.456712e-48 | 6.779143e-46 |
| MsG0480021737.01 | MsG0580029507.01 | 0.813251 | 2.813665e-51 | 1.828006e-48 |
| MsG0480021737.01 | MsG0680030549.01 | 0.860968 | 1.426446e-63 | 3.970770e-60 |
| MsG0480021737.01 | MsG0680030964.01 | 0.848653 | 5.344602e-60 | 9.876530e-57 |
| MsG0480021737.01 | MsG0680031704.01 | 0.801417 | 9.095318e-49 | 4.341214e-46 |
| MsG0480021737.01 | MsG0680034235.01 | 0.802124 | 6.510826e-49 | 3.163803e-46 |
| MsG0480021737.01 | MsG0680035307.01 | 0.804173 | 2.453303e-49 | 1.256331e-46 |
| MsG0480021737.01 | MsG0780036263.01 | 0.848231 | 6.992079e-60 | 1.274502e-56 |
| MsG0480021737.01 | MsG0780037399.01 | 0.807769 | 4.299040e-50 | 2.415199e-47 |
| MsG0480021737.01 | MsG0780037946.01 | 0.807838 | 4.156177e-50 | 2.339145e-47 |
| MsG0480021737.01 | MsG0780039994.01 | 0.837804 | 4.186388e-57 | 5.500154e-54 |
| MsG0480021737.01 | MsG0880042035.01 | 0.801255 | 9.816586e-49 | 4.666482e-46 |
| MsG0480021737.01 | MsG0880044105.01 | 0.833059 | 6.626778e-56 | 7.546149e-53 |
| MsG0480021737.01 | MsG0880044884.01 | 0.807330 | 5.328995e-50 | 2.959889e-47 |
| MsG0480021737.01 | MsG0880047152.01 | 0.814924 | 1.202245e-51 | 8.172978e-49 |
| MsG0380015745.01 | MsG0480021737.01 | 0.825083 | 5.688225e-54 | 5.132809e-51 |
| MsG0380016963.01 | MsG0480021737.01 | 0.858008 | 1.106328e-62 | 2.783219e-59 |
| MsG0380017215.01 | MsG0480021737.01 | 0.861915 | 7.333003e-64 | 2.108272e-60 |
| MsG0480018345.01 | MsG0480021737.01 | 0.807248 | 5.545748e-50 | 3.073743e-47 |
| MsG0280006465.01 | MsG0480021737.01 | 0.851716 | 7.404475e-61 | 1.512155e-57 |
| MsG0280006669.01 | MsG0480021737.01 | 0.818693 | 1.715623e-52 | 1.293236e-49 |
| MsG0280006913.01 | MsG0480021737.01 | 0.801962 | 7.029315e-49 | 3.401675e-46 |
| MsG0280008932.01 | MsG0480021737.01 | 0.810003 | 1.430579e-50 | 8.525961e-48 |
| MsG0280009447.01 | MsG0480021737.01 | 0.847087 | 1.443830e-59 | 2.537814e-56 |
| MsG0280010198.01 | MsG0480021737.01 | 0.831237 | 1.870931e-55 | 2.018053e-52 |
| MsG0280010254.01 | MsG0480021737.01 | 0.820964 | 5.193567e-53 | 4.170265e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021737.01.T01 | MTR_4g108040 | 92.405 | 79 | 6 | 0 | 2 | 80 | 244 | 322 | 1.91e-44 | 146 |
| MsG0480021737.01.T01 | MTR_4g074370 | 92.500 | 80 | 6 | 0 | 1 | 80 | 587 | 666 | 1.14e-43 | 149 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAATCATTTATGGATGTTT+TGG | 0.240494 | 4:-63724767 | MsG0480021737.01.T01:CDS |
| GATAATTCTGAAGGTTCTTC+TGG | 0.258885 | 4:-63724601 | MsG0480021737.01.T01:CDS |
| GGCAGATAAAGAATCATTTA+TGG | 0.272115 | 4:-63724776 | MsG0480021737.01.T01:CDS |
| TTTACCAAAGGTTTGGATAA+TGG | 0.313194 | 4:-63724691 | MsG0480021737.01.T01:CDS |
| GGGATAGTAGCATCTGAAAC+AGG | 0.328126 | 4:-63724715 | MsG0480021737.01.T01:CDS |
| AATTAGAGATTCTAAGAGTA+TGG | 0.329702 | 4:+63724573 | None:intergenic |
| AACAGGGTTTACCAAAGGTT+TGG | 0.361166 | 4:-63724698 | MsG0480021737.01.T01:CDS |
| TTGGATAATGGCTCAAATAC+AGG | 0.400332 | 4:-63724679 | MsG0480021737.01.T01:CDS |
| TGAGCCATTATCCAAACCTT+TGG | 0.412143 | 4:+63724687 | None:intergenic |
| ATTGATTCCGCATTGGCAAC+AGG | 0.413301 | 4:+63724637 | None:intergenic |
| ATAAATGATTCTTTATCTGC+CGG | 0.434471 | 4:+63724778 | None:intergenic |
| TTTCAGATGCTACTATCCCT+TGG | 0.481868 | 4:+63724719 | None:intergenic |
| CGTAAGAGATTCTGCGTACC+CGG | 0.493051 | 4:-63724797 | MsG0480021737.01.T01:CDS |
| TAAATGATTCTTTATCTGCC+GGG | 0.543828 | 4:+63724779 | None:intergenic |
| AGGAGGAATTGATTCCGCAT+TGG | 0.577509 | 4:+63724630 | None:intergenic |
| CCTAGTGCTGATAATTCTGA+AGG | 0.579399 | 4:-63724610 | MsG0480021737.01.T01:CDS |
| ATTGTTAGCGAAGAATTCCA+AGG | 0.582227 | 4:-63724736 | MsG0480021737.01.T01:CDS |
| CCTTCAGAATTATCAGCACT+AGG | 0.583333 | 4:+63724610 | None:intergenic |
| TGGATAATGGCTCAAATACA+GGG | 0.600302 | 4:-63724678 | MsG0480021737.01.T01:CDS |
| TCTGAAACAGGGTTTACCAA+AGG | 0.606194 | 4:-63724703 | MsG0480021737.01.T01:CDS |
| AATTCTGAAGGTTCTTCTGG+AGG | 0.615378 | 4:-63724598 | MsG0480021737.01.T01:CDS |
| AAAATCTCCTGTTGCCAATG+CGG | 0.628618 | 4:-63724644 | MsG0480021737.01.T01:CDS |
| TTGTTAGCGAAGAATTCCAA+GGG | 0.640177 | 4:-63724735 | MsG0480021737.01.T01:CDS |
| GGATAGTAGCATCTGAAACA+GGG | 0.653128 | 4:-63724714 | MsG0480021737.01.T01:CDS |
| TCAGAATTATCAGCACTAGG+AGG | 0.678287 | 4:+63724613 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATAAATGATTCTTTATCTGC+CGG | + | Chr4:63724597-63724616 | None:intergenic | 25.0% |
| !! | AATTAGAGATTCTAAGAGTA+TGG | + | Chr4:63724802-63724821 | None:intergenic | 25.0% |
| !! | AGAATCATTTATGGATGTTT+TGG | - | Chr4:63724605-63724624 | MsG0480021737.01.T01:CDS | 25.0% |
| GGCAGATAAAGAATCATTTA+TGG | - | Chr4:63724596-63724615 | MsG0480021737.01.T01:CDS | 30.0% | |
| TAAATGATTCTTTATCTGCC+GGG | + | Chr4:63724596-63724615 | None:intergenic | 30.0% | |
| TTTACCAAAGGTTTGGATAA+TGG | - | Chr4:63724681-63724700 | MsG0480021737.01.T01:CDS | 30.0% | |
| ATTGTTAGCGAAGAATTCCA+AGG | - | Chr4:63724636-63724655 | MsG0480021737.01.T01:CDS | 35.0% | |
| TTGTTAGCGAAGAATTCCAA+GGG | - | Chr4:63724637-63724656 | MsG0480021737.01.T01:CDS | 35.0% | |
| ! | GATAATTCTGAAGGTTCTTC+TGG | - | Chr4:63724771-63724790 | MsG0480021737.01.T01:CDS | 35.0% |
| ! | TGGATAATGGCTCAAATACA+GGG | - | Chr4:63724694-63724713 | MsG0480021737.01.T01:CDS | 35.0% |
| ! | TTGGATAATGGCTCAAATAC+AGG | - | Chr4:63724693-63724712 | MsG0480021737.01.T01:CDS | 35.0% |
| AAAATCTCCTGTTGCCAATG+CGG | - | Chr4:63724728-63724747 | MsG0480021737.01.T01:CDS | 40.0% | |
| AACAGGGTTTACCAAAGGTT+TGG | - | Chr4:63724674-63724693 | MsG0480021737.01.T01:CDS | 40.0% | |
| CCTTCAGAATTATCAGCACT+AGG | + | Chr4:63724765-63724784 | None:intergenic | 40.0% | |
| TCAGAATTATCAGCACTAGG+AGG | + | Chr4:63724762-63724781 | None:intergenic | 40.0% | |
| TGAGCCATTATCCAAACCTT+TGG | + | Chr4:63724688-63724707 | None:intergenic | 40.0% | |
| TTTCAGATGCTACTATCCCT+TGG | + | Chr4:63724656-63724675 | None:intergenic | 40.0% | |
| ! | AATTCTGAAGGTTCTTCTGG+AGG | - | Chr4:63724774-63724793 | MsG0480021737.01.T01:CDS | 40.0% |
| ! | GGATAGTAGCATCTGAAACA+GGG | - | Chr4:63724658-63724677 | MsG0480021737.01.T01:CDS | 40.0% |
| ! | TCTGAAACAGGGTTTACCAA+AGG | - | Chr4:63724669-63724688 | MsG0480021737.01.T01:CDS | 40.0% |
| !! | CCTAGTGCTGATAATTCTGA+AGG | - | Chr4:63724762-63724781 | MsG0480021737.01.T01:CDS | 40.0% |
| ! | AGGAGGAATTGATTCCGCAT+TGG | + | Chr4:63724745-63724764 | None:intergenic | 45.0% |
| ! | ATTGATTCCGCATTGGCAAC+AGG | + | Chr4:63724738-63724757 | None:intergenic | 45.0% |
| ! | GGGATAGTAGCATCTGAAAC+AGG | - | Chr4:63724657-63724676 | MsG0480021737.01.T01:CDS | 45.0% |
| CGTAAGAGATTCTGCGTACC+CGG | - | Chr4:63724575-63724594 | MsG0480021737.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 63724570 | 63724824 | 63724570 | ID=MsG0480021737.01;Name=MsG0480021737.01 |
| Chr4 | mRNA | 63724570 | 63724824 | 63724570 | ID=MsG0480021737.01.T01;Parent=MsG0480021737.01;Name=MsG0480021737.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|84 |
| Chr4 | exon | 63724570 | 63724824 | 63724570 | ID=MsG0480021737.01.T01:exon:3525;Parent=MsG0480021737.01.T01 |
| Chr4 | CDS | 63724570 | 63724824 | 63724570 | ID=MsG0480021737.01.T01:cds;Parent=MsG0480021737.01.T01 |
| Gene Sequence |
| Protein sequence |
