AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021871.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021871.01.T01 MTR_4g478190 88.865 458 18 2 1 425 1 458 0.0 766
MsG0480021871.01.T01 MTR_5g007350 64.706 459 125 7 2 424 26 483 3.98e-178 520
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021871.01.T01 AT1G65010 49.180 427 181 4 33 425 30 454 2.64e-103 334
MsG0480021871.01.T01 AT4G27595 46.771 449 184 9 1 409 1 434 1.17e-102 331
MsG0480021871.01.T01 AT4G27595 47.248 436 181 8 8 409 5 425 8.30e-102 328
MsG0480021871.01.T01 AT5G16730 47.126 435 183 9 20 413 24 452 8.66e-89 288
MsG0480021871.01.T01 AT3G02930 48.786 453 188 10 1 417 1 445 5.20e-85 276
MsG0480021871.01.T01 AT3G02930 48.786 453 188 10 1 417 1 445 5.20e-85 276
MsG0480021871.01.T01 AT3G02930 48.661 448 188 9 6 417 2 443 8.64e-85 276

Find 91 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATTGGTTTGAAATTAT+TGG 0.226649 4:+65409943 None:intergenic
CTGCTTTCAACTTCAATATA+AGG 0.227623 4:+65407563 None:intergenic
TCAATGAATTGGAGAATTCT+AGG 0.267794 4:-65407012 MsG0480021871.01.T01:CDS
GAGAAACACAACAACAATTT+AGG 0.280321 4:+65409885 None:intergenic
GTAGTAAGTTAGAAGAATTA+AGG 0.298792 4:+65409914 None:intergenic
TTGCTAACTTTACTTACTTT+CGG 0.314225 4:+65409119 None:intergenic
TTCAGAACGCCGTCTTCTTA+TGG 0.322768 4:-65407185 MsG0480021871.01.T01:CDS
TACTTGTTACTTTGCTTCCA+TGG 0.346474 4:-65409795 MsG0480021871.01.T01:exon
TTACTTACTTTCGGTGTTGC+CGG 0.350236 4:+65409128 None:intergenic
TCTGAACCCTTTGCAGCTCT+TGG 0.352340 4:+65408100 None:intergenic
GAAAGATAATGTGGCCTGTC+TGG 0.352818 4:-65410031 MsG0480021871.01.T01:five_prime_UTR
AAACAAACTCATCAATGAAT+TGG 0.355092 4:-65407023 MsG0480021871.01.T01:CDS
AGTTAGAAGAATTAAGGTTT+TGG 0.370347 4:+65409920 None:intergenic
TGGCGACTCAGATGCTGATT+TGG 0.373526 4:+65409088 None:intergenic
AAAATTCCGAGCTGTTGAAT+TGG 0.380433 4:-65407887 MsG0480021871.01.T01:CDS
TTCCTTAACTGCCTCAAGTT+CGG 0.393340 4:+65407114 None:intergenic
TGTTGCCGGTGGTGCTTTGT+TGG 0.402178 4:+65409142 None:intergenic
CAATATAAGGTTATTATCAC+TGG 0.402454 4:+65407576 None:intergenic
GCTGATTTCCGCTCAACAGC+TGG 0.414676 4:+65409005 None:intergenic
ATCCGAACTTGAGGCAGTTA+AGG 0.419625 4:-65407116 MsG0480021871.01.T01:CDS
TGGTGCTTTGTTGGAATTGT+CGG 0.419748 4:+65409151 None:intergenic
AGCTGAATTGACGCAGTTGA+AGG 0.430970 4:-65407629 MsG0480021871.01.T01:CDS
CCGAGCTGTTGAATTGGAAC+AGG 0.431367 4:-65407881 MsG0480021871.01.T01:CDS
ACCTTCTCCTTCTCAGCTGA+AGG 0.434987 4:+65408020 None:intergenic
TCAGATGCTGATTTGGTCAC+TGG 0.435750 4:+65409095 None:intergenic
TTCATGTGGTTGTTCCAGAC+AGG 0.448414 4:+65410017 None:intergenic
GAGATCTGCATCAGAATCCT+TGG 0.462775 4:-65407338 MsG0480021871.01.T01:CDS
CTCAAGCTCCTCAGTGGTTG+AGG 0.472269 4:+65407765 None:intergenic
CCACTCCTCTAGCAGACTGT+TGG 0.480622 4:+65407414 None:intergenic
CATCAGCATGATTTAATGCC+TGG 0.490454 4:+65407701 None:intergenic
TGGCTTCAAAATCTAGTACT+AGG 0.491809 4:-65409775 MsG0480021871.01.T01:intron
AGCAGATCCTTCAGCTGAGA+AGG 0.492213 4:-65408027 MsG0480021871.01.T01:CDS
CTGCTTCATTACAGATTCCA+AGG 0.493723 4:+65407321 None:intergenic
TTGAAATTATTGGAAGGAGA+AGG 0.495882 4:+65409953 None:intergenic
ACAAGCTCCAAGAGCTGCAA+AGG 0.501010 4:-65408107 MsG0480021871.01.T01:CDS
CTATTATTGCTTACATCAGG+TGG 0.516293 4:+65408966 None:intergenic
GCAAAATTCACGTCTTTCTG+TGG 0.518226 4:-65409057 MsG0480021871.01.T01:CDS
AGTTGAGGAACTAGAAATGA+GGG 0.521126 4:-65407383 MsG0480021871.01.T01:CDS
TTCTTGTGTTGAATCTAGCA+AGG 0.527152 4:+65407606 None:intergenic
GGTTGAAGAATCAAACAAGT+TGG 0.530427 4:-65407362 MsG0480021871.01.T01:CDS
CAATTCCAACAAAGCACCAC+CGG 0.535286 4:-65409147 MsG0480021871.01.T01:CDS
AACCACTGAGGAGCTTGAGC+GGG 0.538320 4:-65407761 MsG0480021871.01.T01:CDS
CTCAGTGGTTGAGGCAAGAG+TGG 0.539143 4:+65407774 None:intergenic
TGAATAAGAAGGAAGAAGAA+TGG 0.541280 4:-65407843 MsG0480021871.01.T01:CDS
AAGTTGAGGAACTAGAAATG+AGG 0.541760 4:-65407384 MsG0480021871.01.T01:CDS
AGTTAAGGAAGAGAAAGATC+AGG 0.542809 4:-65407101 MsG0480021871.01.T01:CDS
TGCTACTCTTAAAGAGAAGG+TGG 0.543694 4:-65407254 MsG0480021871.01.T01:CDS
ACGCCGTCTTCTTATGGCTA+AGG 0.545598 4:-65407179 MsG0480021871.01.T01:CDS
GCTGTTGAATTGGAACAGGC+TGG 0.548085 4:-65407877 MsG0480021871.01.T01:CDS
TGGCCACATCCAAGGCATGC+TGG 0.548388 4:+65407794 None:intergenic
GGCTTTGAATTCACAGATCG+TGG 0.550956 4:+65409026 None:intergenic
ATTGGTTTGAAATTATTGGA+AGG 0.551236 4:+65409947 None:intergenic
TGTGAGGAACCAGCATGCCT+TGG 0.551371 4:-65407803 MsG0480021871.01.T01:CDS
GCTTACATCAGGTGGGGTGG+CGG 0.552794 4:+65408974 None:intergenic
CAATGAATTGGAGAATTCTA+GGG 0.553198 4:-65407011 MsG0480021871.01.T01:CDS
TATTATTGCTTACATCAGGT+GGG 0.554905 4:+65408967 None:intergenic
AATTGAAACTGTGAATAAGA+AGG 0.556477 4:-65407854 MsG0480021871.01.T01:CDS
CAACCACTGAGGAGCTTGAG+CGG 0.561441 4:-65407762 MsG0480021871.01.T01:CDS
CTTCCTTAGCCATAAGAAGA+CGG 0.564152 4:+65407176 None:intergenic
TACTTTCTCAGCATGAACCT+CGG 0.567598 4:+65407663 None:intergenic
TGAAAAGTCAAAGAAGATTG+AGG 0.568592 4:-65407146 MsG0480021871.01.T01:CDS
GGCAAGAGTGGCCACATCCA+AGG 0.575724 4:+65407786 None:intergenic
GTTCTTAGCATCACACATCA+TGG 0.582449 4:+65407723 None:intergenic
AGCTGCTACTCTTAAAGAGA+AGG 0.582698 4:-65407257 MsG0480021871.01.T01:CDS
ATTATTGCTTACATCAGGTG+GGG 0.583904 4:+65408968 None:intergenic
GCTTTGTTGGAATTGTCGGA+TGG 0.586226 4:+65409155 None:intergenic
AACGGTTAAGAAAGATAATG+TGG 0.587116 4:-65410040 MsG0480021871.01.T01:five_prime_UTR
AGAAGATCTAAAGAAAGCAA+AGG 0.589350 4:-65408050 MsG0480021871.01.T01:CDS
ATGTAGAACTTGAAACCTCA+AGG 0.589981 4:-65407453 MsG0480021871.01.T01:CDS
GAACCAGCATGCCTTGGATG+TGG 0.590468 4:-65407797 MsG0480021871.01.T01:CDS
AGGGAATGAGTTATGATGAG+AGG 0.592178 4:-65407510 MsG0480021871.01.T01:CDS
GCGTTCTGAATCCTCAAGAT+CGG 0.596202 4:+65407198 None:intergenic
GGCTCTTGAATCCGAACTTG+AGG 0.596607 4:-65407125 MsG0480021871.01.T01:CDS
CAAGCTCCAAGAGCTGCAAA+GGG 0.600838 4:-65408106 MsG0480021871.01.T01:CDS
CTTACTTTCGGTGTTGCCGG+TGG 0.601427 4:+65409131 None:intergenic
CCTGTTCCAATTCAACAGCT+CGG 0.607837 4:+65407881 None:intergenic
ATTGCTTACATCAGGTGGGG+TGG 0.615277 4:+65408971 None:intergenic
TTATGCCAACAGTCTGCTAG+AGG 0.620146 4:-65407419 MsG0480021871.01.T01:CDS
TCCTTCAGCTGAGAAGGAGA+AGG 0.623888 4:-65408021 MsG0480021871.01.T01:intron
TAATAGGGTCTGCACATGAG+AGG 0.625836 4:+65407054 None:intergenic
CCAACAGTCTGCTAGAGGAG+TGG 0.633139 4:-65407414 MsG0480021871.01.T01:CDS
AACCCGCTCAAGCTCCTCAG+TGG 0.633744 4:+65407759 None:intergenic
CTCAAGAAAGAACTCGACAA+GGG 0.633999 4:-65407529 MsG0480021871.01.T01:CDS
TCTCAAGAAAGAACTCGACA+AGG 0.634380 4:-65407530 MsG0480021871.01.T01:CDS
ATAAGATTCAGCCGTCCTTG+AGG 0.636404 4:+65407438 None:intergenic
ATTCAAAGCCAGCTGTTGAG+CGG 0.660562 4:-65409013 MsG0480021871.01.T01:CDS
GCTACTCTTAAAGAGAAGGT+GGG 0.661481 4:-65407253 MsG0480021871.01.T01:CDS
TGATGCAGCTAAAGTTGCCG+AGG 0.669959 4:-65407680 MsG0480021871.01.T01:CDS
AGAACTTGAAACCTCAAGGA+CGG 0.671436 4:-65407449 MsG0480021871.01.T01:CDS
CACTCTTGCCTCAACCACTG+AGG 0.695834 4:-65407773 MsG0480021871.01.T01:CDS
GATGTGTGATGCTAAGAACC+AGG 0.701568 4:-65407719 MsG0480021871.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATAGTAATTGAAATGA+AGG + Chr4:65408190-65408209 None:intergenic 15.0%
!! AAAGTAAAAAACAACAAAAA+TGG + Chr4:65407387-65407406 None:intergenic 15.0%
!!! TAATAATTTGACATTTTTGT+GGG - Chr4:65408311-65408330 MsG0480021871.01.T01:intron 15.0%
!!! TCATTTCAATTACTATTTTT+GGG - Chr4:65408190-65408209 MsG0480021871.01.T01:intron 15.0%
!!! TTCATTTCAATTACTATTTT+TGG - Chr4:65408189-65408208 MsG0480021871.01.T01:intron 15.0%
!! ATTCTGTAATGTCTATTATT+TGG - Chr4:65408374-65408393 MsG0480021871.01.T01:intron 20.0%
!! CAACAATGTATTGTAAAATA+AGG + Chr4:65407663-65407682 None:intergenic 20.0%
!! CTATGTACAATTCAAAAATT+GGG - Chr4:65407565-65407584 MsG0480021871.01.T01:CDS 20.0%
!! GTAAAATACTGTTAAACATA+AGG + Chr4:65408434-65408453 None:intergenic 20.0%
!! TCTATGTACAATTCAAAAAT+TGG - Chr4:65407564-65407583 MsG0480021871.01.T01:CDS 20.0%
!!! AAATAAGAATTGTTTTCTTG+TGG - Chr4:65407747-65407766 MsG0480021871.01.T01:CDS 20.0%
!!! AATAATTTGACATTTTTGTG+GGG - Chr4:65408312-65408331 MsG0480021871.01.T01:intron 20.0%
!!! CTAATAATTTGACATTTTTG+TGG - Chr4:65408310-65408329 MsG0480021871.01.T01:intron 20.0%
!!! TGAAATTGGTTTGAAATTAT+TGG + Chr4:65407110-65407129 None:intergenic 20.0%
!!! TGTTGTTTTTTACTTTTTGT+CGG - Chr4:65407391-65407410 MsG0480021871.01.T01:CDS 20.0%
!!! TTTGTTTTTCTCTTCTAATA+GGG + Chr4:65410014-65410033 None:intergenic 20.0%
!!! TTTTTTTGTTGAACTATGAA+TGG + Chr4:65407218-65407237 None:intergenic 20.0%
! AAACAAACTCATCAATGAAT+TGG - Chr4:65410027-65410046 MsG0480021871.01.T01:five_prime_UTR 25.0%
! AAGAAGAAGAAAAAGCAAAA+AGG - Chr4:65410062-65410081 MsG0480021871.01.T01:five_prime_UTR 25.0%
! AATAGACATTACAGAATGAA+AGG + Chr4:65408371-65408390 None:intergenic 25.0%
! AATCTATTATTGCTTACATC+AGG + Chr4:65408090-65408109 None:intergenic 25.0%
! AATTGAAACTGTGAATAAGA+AGG - Chr4:65409196-65409215 MsG0480021871.01.T01:intron 25.0%
! ATACTGTTAAGATCTGAATT+GGG - Chr4:65407335-65407354 MsG0480021871.01.T01:CDS 25.0%
! CAATATAAGGTTATTATCAC+TGG + Chr4:65409477-65409496 None:intergenic 25.0%
! GTAGTAAGTTAGAAGAATTA+AGG + Chr4:65407139-65407158 None:intergenic 25.0%
! TAAGTAAAAACAAGTGACAT+GGG + Chr4:65408725-65408744 None:intergenic 25.0%
! TATACTGTTAAGATCTGAAT+TGG - Chr4:65407334-65407353 MsG0480021871.01.T01:CDS 25.0%
! TCAATTTCCAATCAAATAGA+TGG + Chr4:65407780-65407799 None:intergenic 25.0%
! TGTAATGTCTATTATTTGGT+TGG - Chr4:65408378-65408397 MsG0480021871.01.T01:intron 25.0%
! TGTACTTATTATGTATTGCT+TGG - Chr4:65408908-65408927 MsG0480021871.01.T01:intron 25.0%
! TTAAGTAAAAACAAGTGACA+TGG + Chr4:65408726-65408745 None:intergenic 25.0%
! TTGCTAACTTTACTTACTTT+CGG + Chr4:65407934-65407953 None:intergenic 25.0%
!! AGTTAGAAGAATTAAGGTTT+TGG + Chr4:65407133-65407152 None:intergenic 25.0%
!! ATGTTCATCTGTTGAAATTT+TGG - Chr4:65407519-65407538 MsG0480021871.01.T01:CDS 25.0%
!! TGATTTTGTACAATTAAGCT+TGG - Chr4:65407633-65407652 MsG0480021871.01.T01:CDS 25.0%
!!! AATTAAGGTTTTGGTGAAAT+TGG + Chr4:65407124-65407143 None:intergenic 25.0%
!!! ATTGGTTTGAAATTATTGGA+AGG + Chr4:65407106-65407125 None:intergenic 25.0%
!!! GTTTGTTTTTCTCTTCTAAT+AGG + Chr4:65410015-65410034 None:intergenic 25.0%
!!! TGTAATTTGTTTTTTGCTTG+AGG - Chr4:65407414-65407433 MsG0480021871.01.T01:CDS 25.0%
!!! TTGTGTTTTGTTATCTTGAA+TGG - Chr4:65407809-65407828 MsG0480021871.01.T01:CDS 25.0%
AACGGTTAAGAAAGATAATG+TGG - Chr4:65407010-65407029 MsG0480021871.01.T01:CDS 30.0%
AAGAAAAAGCAAAAAGGCAA+TGG - Chr4:65410068-65410087 MsG0480021871.01.T01:five_prime_UTR 30.0%
AATACTGTTAAACATAAGGC+AGG + Chr4:65408430-65408449 None:intergenic 30.0%
AATTAAGCATCCAGAATCAT+TGG + Chr4:65407725-65407744 None:intergenic 30.0%
AATTGACAGCAAAAAAGAAG+TGG + Chr4:65408609-65408628 None:intergenic 30.0%
AGAAGATCTAAAGAAAGCAA+AGG - Chr4:65409000-65409019 MsG0480021871.01.T01:CDS 30.0%
AGTAAAATTCGACTCTTGAT+TGG - Chr4:65408786-65408805 MsG0480021871.01.T01:intron 30.0%
ATGTGAGAGAGAAAAAAGAA+CGG - Chr4:65406992-65407011 MsG0480021871.01.T01:CDS 30.0%
CAATGAATTGGAGAATTCTA+GGG - Chr4:65410039-65410058 MsG0480021871.01.T01:five_prime_UTR 30.0%
CACTAGATATTTGAATCAAC+TGG + Chr4:65408498-65408517 None:intergenic 30.0%
CAGAAACTGAAAAATTGAGT+TGG + Chr4:65407061-65407080 None:intergenic 30.0%
CTGCTTTCAACTTCAATATA+AGG + Chr4:65409490-65409509 None:intergenic 30.0%
CTTAGATCCATCTATTTGAT+TGG - Chr4:65407770-65407789 MsG0480021871.01.T01:CDS 30.0%
GAGAAACACAACAACAATTT+AGG + Chr4:65407168-65407187 None:intergenic 30.0%
GATTCAAGTCATAAACCAAA+TGG + Chr4:65408658-65408677 None:intergenic 30.0%
GTAAAATTCGACTCTTGATT+GGG - Chr4:65408787-65408806 MsG0480021871.01.T01:intron 30.0%
TATTATTGCTTACATCAGGT+GGG + Chr4:65408086-65408105 None:intergenic 30.0%
TCAATGAATTGGAGAATTCT+AGG - Chr4:65410038-65410057 MsG0480021871.01.T01:five_prime_UTR 30.0%
TGAAAAGTCAAAGAAGATTG+AGG - Chr4:65409904-65409923 MsG0480021871.01.T01:five_prime_UTR 30.0%
TGAATAAGAAGGAAGAAGAA+TGG - Chr4:65409207-65409226 MsG0480021871.01.T01:intron 30.0%
TGTCTATTATTTGGTTGGAT+TGG - Chr4:65408383-65408402 MsG0480021871.01.T01:intron 30.0%
TTGAAATTATTGGAAGGAGA+AGG + Chr4:65407100-65407119 None:intergenic 30.0%
! TTATCATGCTCTCTTTTGAA+AGG - Chr4:65408870-65408889 MsG0480021871.01.T01:intron 30.0%
! TTTTGTTGAACTATGAATGG+TGG + Chr4:65407215-65407234 None:intergenic 30.0%
!! TATTATTTGGTTGGATTGGT+TGG - Chr4:65408387-65408406 MsG0480021871.01.T01:intron 30.0%
!!! AAATTGAGTTGGTTTTCATG+TGG + Chr4:65407050-65407069 None:intergenic 30.0%
!!! AATTTGTTTTTTGCTTGAGG+AGG - Chr4:65407417-65407436 MsG0480021871.01.T01:CDS 30.0%
!!! GAATGGTGTGTTACTTTTAA+TGG - Chr4:65407826-65407845 MsG0480021871.01.T01:CDS 30.0%
AAAATTCCGAGCTGTTGAAT+TGG - Chr4:65409163-65409182 MsG0480021871.01.T01:CDS 35.0%
AAGTTGAGGAACTAGAAATG+AGG - Chr4:65409666-65409685 MsG0480021871.01.T01:intron 35.0%
AGTTAAGGAAGAGAAAGATC+AGG - Chr4:65409949-65409968 MsG0480021871.01.T01:five_prime_UTR 35.0%
AGTTGAGGAACTAGAAATGA+GGG - Chr4:65409667-65409686 MsG0480021871.01.T01:intron 35.0%
ATGTAGAACTTGAAACCTCA+AGG - Chr4:65409597-65409616 MsG0480021871.01.T01:intron 35.0%
ATTATTGCTTACATCAGGTG+GGG + Chr4:65408085-65408104 None:intergenic 35.0%
CTATTATTGCTTACATCAGG+TGG + Chr4:65408087-65408106 None:intergenic 35.0%
GGTTGAAGAATCAAACAAGT+TGG - Chr4:65409688-65409707 MsG0480021871.01.T01:intron 35.0%
TATCGAATCGAGATATGACT+CGG + Chr4:65408464-65408483 None:intergenic 35.0%
TGGCTTCAAAATCTAGTACT+AGG - Chr4:65407275-65407294 MsG0480021871.01.T01:CDS 35.0%
TTCAGTAGACTTGAGATGTT+AGG + Chr4:65408233-65408252 None:intergenic 35.0%
TTCTTGTGTTGAATCTAGCA+AGG + Chr4:65409447-65409466 None:intergenic 35.0%
! AAAAAGAGCTTGAAAGTGTG+AGG - Chr4:65409231-65409250 MsG0480021871.01.T01:intron 35.0%
! AGTACTAGGTTAGTCAAGTT+TGG - Chr4:65407289-65407308 MsG0480021871.01.T01:CDS 35.0%
! GAAAGACGTGAATTTTGCAA+CGG + Chr4:65407991-65408010 None:intergenic 35.0%
! GTCTACTGAAAAGTGTGATA+AGG - Chr4:65408243-65408262 MsG0480021871.01.T01:intron 35.0%
!! TACTTGTTACTTTGCTTCCA+TGG - Chr4:65407255-65407274 MsG0480021871.01.T01:CDS 35.0%
!!! AGTACTAGATTTTGAAGCCA+TGG + Chr4:65407275-65407294 None:intergenic 35.0%
AGAACTTGAAACCTCAAGGA+CGG - Chr4:65409601-65409620 MsG0480021871.01.T01:intron 40.0%
AGCTGCTACTCTTAAAGAGA+AGG - Chr4:65409793-65409812 MsG0480021871.01.T01:exon 40.0%
AGGGAATGAGTTATGATGAG+AGG - Chr4:65409540-65409559 MsG0480021871.01.T01:intron 40.0%
CATCAGCATGATTTAATGCC+TGG + Chr4:65409352-65409371 None:intergenic 40.0%
CTCAAGAAAGAACTCGACAA+GGG - Chr4:65409521-65409540 MsG0480021871.01.T01:intron 40.0%
CTGCTTCATTACAGATTCCA+AGG + Chr4:65409732-65409751 None:intergenic 40.0%
CTTCCTTAGCCATAAGAAGA+CGG + Chr4:65409877-65409896 None:intergenic 40.0%
GAACCATTCTAACGAATCTC+TGG - Chr4:65408564-65408583 MsG0480021871.01.T01:intron 40.0%
GACAGCAAAAAAGAAGTGGT+TGG + Chr4:65408605-65408624 None:intergenic 40.0%
GCAAAATTCACGTCTTTCTG+TGG - Chr4:65407993-65408012 MsG0480021871.01.T01:intron 40.0%
GCTACTCTTAAAGAGAAGGT+GGG - Chr4:65409797-65409816 MsG0480021871.01.T01:exon 40.0%
GGAGTGGAAAAAGAAAGTTG+AGG - Chr4:65409652-65409671 MsG0480021871.01.T01:intron 40.0%
GTTCTTAGCATCACACATCA+TGG + Chr4:65409330-65409349 None:intergenic 40.0%
TACTTTCTCAGCATGAACCT+CGG + Chr4:65409390-65409409 None:intergenic 40.0%
TAGCCAGAGATTCGTTAGAA+TGG + Chr4:65408570-65408589 None:intergenic 40.0%
TCTCAAGAAAGAACTCGACA+AGG - Chr4:65409520-65409539 MsG0480021871.01.T01:intron 40.0%
TGCTACTCTTAAAGAGAAGG+TGG - Chr4:65409796-65409815 MsG0480021871.01.T01:exon 40.0%
TTCCTTAACTGCCTCAAGTT+CGG + Chr4:65409939-65409958 None:intergenic 40.0%
TTGAACTCGAGATGAGATTC+AGG + Chr4:65408536-65408555 None:intergenic 40.0%
! CAGCTCGGAATTTTTCATCT+CGG + Chr4:65409157-65409176 None:intergenic 40.0%
! GACACTTCTCTTTTGCCATT+TGG - Chr4:65408640-65408659 MsG0480021871.01.T01:intron 40.0%
! GTTGAAAGAAGCACAAAGAG+TGG - Chr4:65409068-65409087 MsG0480021871.01.T01:CDS 40.0%
! TGGTGCTTTGTTGGAATTGT+CGG + Chr4:65407902-65407921 None:intergenic 40.0%
!! GCTGTTGTTTCCAATGATTC+TGG - Chr4:65407712-65407731 MsG0480021871.01.T01:CDS 40.0%
!! TTACTTACTTTCGGTGTTGC+CGG + Chr4:65407925-65407944 None:intergenic 40.0%
AGCTGAATTGACGCAGTTGA+AGG - Chr4:65409421-65409440 MsG0480021871.01.T01:intron 45.0%
ATAAGATTCAGCCGTCCTTG+AGG + Chr4:65409615-65409634 None:intergenic 45.0%
ATCCGAACTTGAGGCAGTTA+AGG - Chr4:65409934-65409953 MsG0480021871.01.T01:five_prime_UTR 45.0%
ATTCAAAGCCAGCTGTTGAG+CGG - Chr4:65408037-65408056 MsG0480021871.01.T01:CDS 45.0%
CCTGTTCCAATTCAACAGCT+CGG + Chr4:65409172-65409191 None:intergenic 45.0%
GAAAGATAATGTGGCCTGTC+TGG - Chr4:65407019-65407038 MsG0480021871.01.T01:CDS 45.0%
GAGACAAAAAGCCGATCTTG+AGG - Chr4:65409841-65409860 MsG0480021871.01.T01:five_prime_UTR 45.0%
GAGATCTGCATCAGAATCCT+TGG - Chr4:65409712-65409731 MsG0480021871.01.T01:intron 45.0%
GAGCTTCTCATTTGCTTCCT+CGG + Chr4:65409094-65409113 None:intergenic 45.0%
GATGTGTGATGCTAAGAACC+AGG - Chr4:65409331-65409350 MsG0480021871.01.T01:intron 45.0%
TAAGGCAGGAACATCTTCAG+CGG + Chr4:65408416-65408435 None:intergenic 45.0%
TAATAGGGTCTGCACATGAG+AGG + Chr4:65409999-65410018 None:intergenic 45.0%
TTATGCCAACAGTCTGCTAG+AGG - Chr4:65409631-65409650 MsG0480021871.01.T01:intron 45.0%
TTCAGAACGCCGTCTTCTTA+TGG - Chr4:65409865-65409884 MsG0480021871.01.T01:five_prime_UTR 45.0%
TTCATGTGGTTGTTCCAGAC+AGG + Chr4:65407036-65407055 None:intergenic 45.0%
! CAATTCCAACAAAGCACCAC+CGG - Chr4:65407903-65407922 MsG0480021871.01.T01:CDS 45.0%
! CGTGAATTTTGCAACGGAGA+TGG + Chr4:65407985-65408004 None:intergenic 45.0%
! GCGTTCTGAATCCTCAAGAT+CGG + Chr4:65409855-65409874 None:intergenic 45.0%
! GCTTTGTTGGAATTGTCGGA+TGG + Chr4:65407898-65407917 None:intergenic 45.0%
! GGAAACATCTGAGATGTGCA+AGG - Chr4:65408264-65408283 MsG0480021871.01.T01:intron 45.0%
!! GGCTTTGAATTCACAGATCG+TGG + Chr4:65408027-65408046 None:intergenic 45.0%
!! TCAGATGCTGATTTGGTCAC+TGG + Chr4:65407958-65407977 None:intergenic 45.0%
ACAAGCTCCAAGAGCTGCAA+AGG - Chr4:65408943-65408962 MsG0480021871.01.T01:intron 50.0%
ACCTTCTCCTTCTCAGCTGA+AGG + Chr4:65409033-65409052 None:intergenic 50.0%
ACGCCGTCTTCTTATGGCTA+AGG - Chr4:65409871-65409890 MsG0480021871.01.T01:five_prime_UTR 50.0%
AGCAGATCCTTCAGCTGAGA+AGG - Chr4:65409023-65409042 MsG0480021871.01.T01:CDS 50.0%
ATTGCTTACATCAGGTGGGG+TGG + Chr4:65408082-65408101 None:intergenic 50.0%
CAAGCTCCAAGAGCTGCAAA+GGG - Chr4:65408944-65408963 MsG0480021871.01.T01:intron 50.0%
CCGAGCTGTTGAATTGGAAC+AGG - Chr4:65409169-65409188 MsG0480021871.01.T01:intron 50.0%
CGACTCTTGATTGGGAAAGC+AGG - Chr4:65408795-65408814 MsG0480021871.01.T01:intron 50.0%
GCTGTTGAATTGGAACAGGC+TGG - Chr4:65409173-65409192 MsG0480021871.01.T01:intron 50.0%
GGAAGCAAATGAGAAGCTCC+AGG - Chr4:65409095-65409114 MsG0480021871.01.T01:CDS 50.0%
GGCTCAAAAGCAGGCTAAAG+AGG - Chr4:65409128-65409147 MsG0480021871.01.T01:CDS 50.0%
GGCTCTTGAATCCGAACTTG+AGG - Chr4:65409925-65409944 MsG0480021871.01.T01:five_prime_UTR 50.0%
TCCTTCAGCTGAGAAGGAGA+AGG - Chr4:65409029-65409048 MsG0480021871.01.T01:CDS 50.0%
TCTGAACCCTTTGCAGCTCT+TGG + Chr4:65408953-65408972 None:intergenic 50.0%
TGATGCAGCTAAAGTTGCCG+AGG - Chr4:65409370-65409389 MsG0480021871.01.T01:intron 50.0%
! TGGCGACTCAGATGCTGATT+TGG + Chr4:65407965-65407984 None:intergenic 50.0%
!! AGCATTGGTGGCTCAAAAGC+AGG - Chr4:65409119-65409138 MsG0480021871.01.T01:CDS 50.0%
AACCACTGAGGAGCTTGAGC+GGG - Chr4:65409289-65409308 MsG0480021871.01.T01:intron 55.0%
CAACCACTGAGGAGCTTGAG+CGG - Chr4:65409288-65409307 MsG0480021871.01.T01:intron 55.0%
CACTCTTGCCTCAACCACTG+AGG - Chr4:65409277-65409296 MsG0480021871.01.T01:intron 55.0%
CCAACAGTCTGCTAGAGGAG+TGG - Chr4:65409636-65409655 MsG0480021871.01.T01:intron 55.0%
CCACTCCTCTAGCAGACTGT+TGG + Chr4:65409639-65409658 None:intergenic 55.0%
CTCAAGCTCCTCAGTGGTTG+AGG + Chr4:65409288-65409307 None:intergenic 55.0%
CTCAGTGGTTGAGGCAAGAG+TGG + Chr4:65409279-65409298 None:intergenic 55.0%
GAACCAGCATGCCTTGGATG+TGG - Chr4:65409253-65409272 MsG0480021871.01.T01:intron 55.0%
TGAGAAGCTCCAGGCAGCAT+TGG - Chr4:65409104-65409123 MsG0480021871.01.T01:CDS 55.0%
TGTGAGGAACCAGCATGCCT+TGG - Chr4:65409247-65409266 MsG0480021871.01.T01:intron 55.0%
TGTTGCCGGTGGTGCTTTGT+TGG + Chr4:65407911-65407930 None:intergenic 55.0%
TTTGAGCCACCAATGCTGCC+TGG + Chr4:65409116-65409135 None:intergenic 55.0%
! AGAAGCACAAAGAGTGGCCG+AGG - Chr4:65409074-65409093 MsG0480021871.01.T01:CDS 55.0%
! GCTGATTTCCGCTCAACAGC+TGG + Chr4:65408048-65408067 None:intergenic 55.0%
!! CTTACTTTCGGTGTTGCCGG+TGG + Chr4:65407922-65407941 None:intergenic 55.0%
AACCCGCTCAAGCTCCTCAG+TGG + Chr4:65409294-65409313 None:intergenic 60.0%
GCTTACATCAGGTGGGGTGG+CGG + Chr4:65408079-65408098 None:intergenic 60.0%
GGCAAGAGTGGCCACATCCA+AGG + Chr4:65409267-65409286 None:intergenic 60.0%
TGGCCACATCCAAGGCATGC+TGG + Chr4:65409259-65409278 None:intergenic 60.0%
! GAAGCTCCAGGCAGCATTGG+TGG - Chr4:65409107-65409126 MsG0480021871.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65406973 65410099 65406973 ID=MsG0480021871.01;Name=MsG0480021871.01
Chr4 mRNA 65406973 65410099 65406973 ID=MsG0480021871.01.T01;Parent=MsG0480021871.01;Name=MsG0480021871.01.T01;_AED=0.48;_eAED=0.48;_QI=301|0.66|0.5|1|0.66|0.75|4|0|429
Chr4 exon 65406973 65407924 65406973 ID=MsG0480021871.01.T01:exon:18074;Parent=MsG0480021871.01.T01
Chr4 exon 65408022 65408131 65408022 ID=MsG0480021871.01.T01:exon:18073;Parent=MsG0480021871.01.T01
Chr4 exon 65408980 65409184 65408980 ID=MsG0480021871.01.T01:exon:18072;Parent=MsG0480021871.01.T01
Chr4 exon 65409776 65410099 65409776 ID=MsG0480021871.01.T01:exon:18071;Parent=MsG0480021871.01.T01
Chr4 five_prime_UTR 65409799 65410099 65409799 ID=MsG0480021871.01.T01:five_prime_utr;Parent=MsG0480021871.01.T01
Chr4 CDS 65409776 65409798 65409776 ID=MsG0480021871.01.T01:cds;Parent=MsG0480021871.01.T01
Chr4 CDS 65408980 65409184 65408980 ID=MsG0480021871.01.T01:cds;Parent=MsG0480021871.01.T01
Chr4 CDS 65408022 65408131 65408022 ID=MsG0480021871.01.T01:cds;Parent=MsG0480021871.01.T01
Chr4 CDS 65406973 65407924 65406973 ID=MsG0480021871.01.T01:cds;Parent=MsG0480021871.01.T01
Gene Sequence

>MsG0480021871.01.T01

ATGGCTTCAAAATCTAGTACTAGATCGAAACCATCCGACAATTCCAACAAAGCACCACCGGCAACACCGAAAGTAAGTAAAGTTAGCAAACCAGTGACCAAATCAGCATCTGAGTCGCCATCTCCGTTGCAAAATTCACGTCTTTCTGTGGAAAAATCGCCACGATCTGTGAATTCAAAGCCAGCTGTTGAGCGGAAATCAGCAAAAGCTACCGCCACCCCACCTGATAAACAAGCTCCAAGAGCTGCAAAGGGTTCAGAGTTGCAGAATCAGTTGAATGTAGCTCAAGAAGATCTAAAGAAAGCAAAGGAGCAGATCCTTCAGCTGAGAAGGAGAAGAGGAATCCGAGATGAAAAATTCCGAGCTGTTGAATTGGAACAGGCTGGAATTGAAACTGTGAATAAGAAGGAAGAAGAATGGCAAAAAGAGCTTGAAAGTGTGAGGAACCAGCATGCCTTGGATGTGGCCACTCTTGCCTCAACCACTGAGGAGCTTGAGCGGGTTAAACAAGAACTCACCATGATGTGTGATGCTAAGAACCAGGCATTAAATCATGCTGATGATGCAGCTAAAGTTGCCGAGGTTCATGCTGAGAAAGTAGAAATTTATGCAGCTGAATTGACGCAGTTGAAGGCCTTGCTAGATTCAACACAAGAAACAAAAGCCAGTGATAATAACCTTATATTGAAGTTGAAAGCAGAGATAGAAGCTCTCAAGAAAGAACTCGACAAGGGAATGAGTTATGATGAGAGGTTGACAGAGAAAGAAACTAAGATTGAACAGCTTAATGTAGAACTTGAAACCTCAAGGACGGCTGAATCTTATGCCAACAGTCTGCTAGAGGAGTGGAAAAAGAAAGTTGAGGAACTAGAAATGAGGGTTGAAGAATCAAACAAGTTGGAGAGATCTGCATCAGAATCCTTGGAATCTGTAATGAAGCAGCTAGAAGAAAGCAATGATCTGTTGCATGACGCAGAATCTGTAGCTGCTACTCTTAAAGAGAAGGTGGGATTGTTAGAAATGACAATTGTGAGACAAAAAGCCGATCTTGAGGATTCAGAACGCCGTCTTCTTATGGCTAAGGAAGAAAATCTTGAAAAGTCAAAGAAGATTGAGGCTCTTGAATCCGAACTTGAGGCAGTTAAGGAAGAGAAAGATCAGGCTTTGAACAATGAGCAGCTTGCAGCCTCTCATGTGCAGACCCTATTAGAAGAGAAAAACAAACTCATCAATGAATTGGAGAATTCTAGGGAAGAAGAAGAAAAAGCAAAAAGGCAATGGAAAGCTTAG
Protein sequence

>MsG0480021871.01.T01

MASKSSTRSKPSDNSNKAPPATPKVSKVSKPVTKSASESPSPLQNSRLSVEKSPRSVNSKPAVERKSAKATATPPDKQAPRAAKGSELQNQLNVAQEDLKKAKEQILQLRRRRGIRDEKFRAVELEQAGIETVNKKEEEWQKELESVRNQHALDVATLASTTEELERVKQELTMMCDAKNQALNHADDAAKVAEVHAEKVEIYAAELTQLKALLDSTQETKASDNNLILKLKAEIEALKKELDKGMSYDERLTEKETKIEQLNVELETSRTAESYANSLLEEWKKKVEELEMRVEESNKLERSASESLESVMKQLEESNDLLHDAESVAATLKEKVGLLEMTIVRQKADLEDSERRLLMAKEENLEKSKKIEALESELEAVKEEKDQALNNEQLAASHVQTLLEEKNKLINELENSREEEEKAKRQWKA*