Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047285.01.T01 | XP_013458374.1 | 93.886 | 229 | 13 | 1 | 1 | 229 | 1 | 228 | 1.24E-116 | 342 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048532.01 | MsG0880047285.01 | 0.867091 | 1.766999e-65 | 6.092975e-62 |
| MsG0080048809.01 | MsG0880047285.01 | 0.810322 | 1.221048e-50 | 7.337622e-48 |
| MsG0180000116.01 | MsG0880047285.01 | -0.823238 | 1.542656e-53 | 1.320755e-50 |
| MsG0180000854.01 | MsG0880047285.01 | -0.802366 | 5.804961e-49 | 2.838471e-46 |
| MsG0180001311.01 | MsG0880047285.01 | -0.835257 | 1.864027e-56 | 2.267292e-53 |
| MsG0180002796.01 | MsG0880047285.01 | -0.820549 | 6.468955e-53 | 5.134233e-50 |
| MsG0180002799.01 | MsG0880047285.01 | -0.803397 | 3.555109e-49 | 1.784647e-46 |
| MsG0180003807.01 | MsG0880047285.01 | -0.832313 | 1.014913e-55 | 1.130049e-52 |
| MsG0180004008.01 | MsG0880047285.01 | -0.820209 | 7.741471e-53 | 6.086693e-50 |
| MsG0180004486.01 | MsG0880047285.01 | -0.819039 | 1.431894e-52 | 1.089819e-49 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047285.01.T01 | MTR_4g123593 | 93.886 | 229 | 13 | 1 | 1 | 229 | 1 | 228 | 1.51e-120 | 342 |
Find 96 sgRNAs with CRISPR-Local
Find 241 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGAAACGGAGACGATTT+TGG | 0.240726 | 8:+84174474 | MsG0880047285.01.T01:CDS |
| AAAGGCGAGGAAGGATCTGA+CGG | 0.645896 | 8:+84174549 | MsG0880047285.01.T01:CDS |
| AACAGGTCTTCCGGGTAGCT+CGG | 0.505808 | 8:-84175840 | None:intergenic |
| AAGGAGCGAGTAATAGTGAT+GGG | 0.520836 | 8:-84174309 | None:intergenic |
| AAGGATCTGACGGTGATAAG+AGG | 0.499849 | 8:+84174559 | MsG0880047285.01.T01:CDS |
| AATTCCATGCAAGTAACCAC+CGG | 0.644415 | 8:-84177139 | None:intergenic |
| AATTGGAAAGAGACAAGTAA+GGG | 0.611539 | 8:-84174353 | None:intergenic |
| ACAAGTGACACAACACCTGC+CGG | 0.564042 | 8:-84177102 | None:intergenic |
| ACCCCAAATTTCATAACAAG+AGG | 0.577194 | 8:-84177231 | None:intergenic |
| ACCTGTTCTTGCATCATCTG+TGG | 0.483230 | 8:+84175856 | MsG0880047285.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAACATTAACACATGAGAGA+AGG | - | Chr8:84176998-84177017 | None:intergenic | 30.0% | |
| AATATGAAGTGTAGAGTTCA+GGG | - | Chr8:84176200-84176219 | None:intergenic | 30.0% | |
| AATTGGAAAGAGACAAGTAA+GGG | - | Chr8:84174356-84174375 | None:intergenic | 30.0% | |
| ACAAATTGTTCAACTGGTTA+TGG | + | Chr8:84176303-84176322 | MsG0880047285.01.T01:intron | 30.0% | |
| AGATAAGTGTTTATAAGTGG+GGG | - | Chr8:84175247-84175266 | None:intergenic | 30.0% | |
| CAATGGAATGATGCATTATT+GGG | - | Chr8:84176676-84176695 | None:intergenic | 30.0% | |
| GGAATTTCATATGTATCAGA+AGG | + | Chr8:84176021-84176040 | MsG0880047285.01.T01:intron | 30.0% | |
| GTAAAAGAGTTTATTCAGAC+AGG | - | Chr8:84175943-84175962 | None:intergenic | 30.0% | |
| TAATATGAAGTGTAGAGTTC+AGG | - | Chr8:84176201-84176220 | None:intergenic | 30.0% | |
| TCGCACTAGAATGTAAATAA+CGG | + | Chr8:84177171-84177190 | MsG0880047285.01.T01:exon | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 84174171 | 84177441 | 84174171 | ID=MsG0880047285.01;Name=MsG0880047285.01 |
| Chr8 | mRNA | 84174171 | 84177441 | 84174171 | ID=MsG0880047285.01.T01;Parent=MsG0880047285.01;Name=MsG0880047285.01.T01;_AED=0.47;_eAED=0.47;_QI=54|1|1|1|1|1|3|262|229 |
| Chr8 | exon | 84174171 | 84174639 | 84174171 | ID=MsG0880047285.01.T01:exon:20938;Parent=MsG0880047285.01.T01 |
| Chr8 | exon | 84175750 | 84175884 | 84175750 | ID=MsG0880047285.01.T01:exon:20939;Parent=MsG0880047285.01.T01 |
| Chr8 | exon | 84177040 | 84177441 | 84177040 | ID=MsG0880047285.01.T01:exon:20940;Parent=MsG0880047285.01.T01 |
| Chr8 | five_prime_UTR | 84174171 | 84174224 | 84174171 | ID=MsG0880047285.01.T01:five_prime_utr;Parent=MsG0880047285.01.T01 |
| Chr8 | CDS | 84174225 | 84174639 | 84174225 | ID=MsG0880047285.01.T01:cds;Parent=MsG0880047285.01.T01 |
| Chr8 | CDS | 84175750 | 84175884 | 84175750 | ID=MsG0880047285.01.T01:cds;Parent=MsG0880047285.01.T01 |
| Chr8 | CDS | 84177040 | 84177179 | 84177040 | ID=MsG0880047285.01.T01:cds;Parent=MsG0880047285.01.T01 |
| Chr8 | three_prime_UTR | 84177180 | 84177441 | 84177180 | ID=MsG0880047285.01.T01:three_prime_utr;Parent=MsG0880047285.01.T01 |
| Gene Sequence |
>MsG0880047285.01.T01 ATGGCAGAAACTGCAGCGGTTTTCGGTGTTTCCAGTTCAACACTTCCTTCCCTCTCTCTTCGCCCTACAACCCGATCCCACCCTCCCATCACTATTACTCGCTCCTTCATCGCTTCTCCTCCTTCTTTCCCTTACTTGTCTCTTTCCAATTCACATTCTCAACTCGCTGTTGTTTCTTCTGATTCCAAAACCAGTCAATTTGACCTAAGTGCCCCCGATTTAGACATTACCGGCGGCGGCGGTGATATCAAAGGAAACGGAGACGATTTTGGCGGAGGAGGTGGTGGTGGTGGTGAAGGAGGAGGCGGAGGAGACGATAGTAACAAAGGCGAGGAAGGATCTGACGGTGATAAGAGGAAGATGGCTTTGTCAATGTCGCAGAAATTAACTCTAGGTTATGCTATTCTCGTTGGAGCGGGTGGTGTAATGGGCTTTCTGAAGAGTGGCAGCCAGAAGTCACTTTTGGCTGGGGGTTTGTCTTCTGCACTGCTGTTTTATGTTTTTACCGAGCTACCCGGAAGACCTGTTCTTGCATCATCTGTGGGCCTTGGCATATCTGCTGCACTTCTTGTGGTGATGGGTTCTCGTTTCAAGAAATCAGGGAAGGTCTTTCCGGCAGGTGTTGTGTCACTTGTGTCGTTCATAATGACCGGTGGTTACTTGCATGGAATTATGCGTAGTTCGCACTAG |
| Protein sequence |
>MsG0880047285.01.T01 MAETAAVFGVSSSTLPSLSLRPTTRSHPPITITRSFIASPPSFPYLSLSNSHSQLAVVSSDSKTSQFDLSAPDLDITGGGGDIKGNGDDFGGGGGGGGEGGGGGDDSNKGEEGSDGDKRKMALSMSQKLTLGYAILVGAGGVMGFLKSGSQKSLLAGGLSSALLFYVFTELPGRPVLASSVGLGISAALLVVMGSRFKKSGKVFPAGVVSLVSFIMTGGYLHGIMRSSH* |
