AlfalfaGEDB | MsG0380018015.01

M. sativa cultivar ZhongmuNo.1 / MsG0380018015.01

KEGG: K11498
GO: GO:0003777 GO:0005524 GO:0007018 GO:0008017
PFAM: PF00225

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380018015.01.T01	XP_003603990.2	68.52	1115	69	15	12	892	12	1078	0	1326

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380018015.01.T01	Q8W5R5	56.095	968	193	19	35	841	28	924	0	926

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000364.01	MsG0380018015.01	0.816162	6.374244e-52	4.482733e-49
MsG0180000741.01	MsG0380018015.01	0.802297	5.998792e-49	2.928075e-46
MsG0180001165.01	MsG0380018015.01	0.808026	3.791633e-50	2.144535e-47
MsG0180003799.01	MsG0380018015.01	0.811830	5.754527e-51	3.599071e-48
MsG0180004437.01	MsG0380018015.01	0.821071	4.905001e-53	3.950384e-50
MsG0180004796.01	MsG0380018015.01	0.808380	3.186694e-50	1.819373e-47
MsG0180005312.01	MsG0380018015.01	0.829207	5.856448e-55	5.954090e-52
MsG0180005451.01	MsG0380018015.01	0.819608	1.062157e-52	8.212176e-50
MsG0180006186.01	MsG0380018015.01	0.806831	6.796022e-50	3.726065e-47
MsG0280010001.01	MsG0380018015.01	0.805200	1.497393e-49	7.873768e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380018015.01.T01	MTR_3g117330	68.520	1115	69	15	12	892	12	1078	0.0	1326
MsG0380018015.01.T01	MTR_4g071210	67.571	885	133	13	1	794	1	822	0.0	1098
MsG0380018015.01.T01	MTR_4g071210	63.095	84	16	2	821	892	992	1072	5.30e-25	112
MsG0380018015.01.T01	MTR_4g071210	67.458	885	133	14	1	794	1	821	0.0	1092
MsG0380018015.01.T01	MTR_4g071210	63.095	84	16	2	821	892	991	1071	5.07e-25	112
MsG0380018015.01.T01	MTR_7g093720	43.121	974	285	19	90	843	64	988	0.0	646
MsG0380018015.01.T01	MTR_1g484960	37.838	481	194	15	100	530	4	429	1.57e-77	275
MsG0380018015.01.T01	MTR_1g017540	40.933	386	159	9	143	512	79	411	6.41e-70	248
MsG0380018015.01.T01	MTR_8g076190	35.135	407	168	9	100	463	20	373	1.12e-55	208
MsG0380018015.01.T01	MTR_5g021650	32.444	487	216	13	80	508	3	434	1.13e-55	208

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380018015.01.T01	AT4G39050	56.095	968	193	19	35	841	28	924	0.0	926
MsG0380018015.01.T01	AT4G39050	58.242	91	22	3	814	892	969	1055	2.16e-22	104
MsG0380018015.01.T01	AT2G21380	60.069	874	184	15	12	794	13	812	0.0	899
MsG0380018015.01.T01	AT2G21380	43.860	171	49	9	742	892	915	1058	3.96e-18	90.5
MsG0380018015.01.T01	AT2G21380	60.069	874	184	15	12	794	13	812	0.0	899
MsG0380018015.01.T01	AT2G21380	43.860	171	49	9	742	892	915	1058	3.96e-18	90.5
MsG0380018015.01.T01	AT1G21730	46.241	878	229	17	98	793	74	890	0.0	634
MsG0380018015.01.T01	AT3G12020	42.992	956	274	18	86	840	54	939	0.0	614
MsG0380018015.01.T01	AT3G12020	42.992	956	274	18	86	840	54	939	0.0	614
MsG0380018015.01.T01	AT5G06670	42.067	958	288	23	90	840	57	954	0.0	597

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Find 170 sgRNAs with CRISPR-Local

Find 638 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATACTTTACAAACAT+GGG	0.513022	3:+103384657	None:intergenic
AAAACATCCTTTATTGCTAA+TGG	0.305400	3:+103392382	None:intergenic
AAACTTGATGCATTTCGTGA+TGG	0.587241	3:-103388291	MsG0380018015.01.T01:CDS
AACAAAAGTCTTCTGACACT+TGG	0.491665	3:-103390537	MsG0380018015.01.T01:CDS
AACAAGAAGATCCATCTGAT+CGG	0.614427	3:+103387585	None:intergenic
AACTTATGTTGAGGGTATAA+AGG	0.372635	3:-103391353	MsG0380018015.01.T01:CDS
AAGAGAAGATTGCAAGAGGC+GGG	0.550419	3:+103390020	None:intergenic
AATATATGCGTCGCGAAATA+AGG	0.315729	3:-103389660	MsG0380018015.01.T01:intron
AATCAAGCGCTTCAGAGTGC+TGG	0.508272	3:+103387522	None:intergenic
AATCGTAACAGAAATGCTAT+CGG	0.649194	3:+103394408	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAAATGCCAAGAATCTG+AGG	+	Chr3:103394570-103394589	None:intergenic	30.0%
AAAAAACTCGATAACACTGT+AGG	+	Chr3:103391771-103391790	None:intergenic	30.0%
AAAAAAGAAGATACAATCCC+AGG	-	Chr3:103392629-103392648	MsG0380018015.01.T01:intron	30.0%
AAAAAATGCCAAGAATCTGA+GGG	+	Chr3:103394569-103394588	None:intergenic	30.0%
AAAATTATCAATGTCGACCA+AGG	+	Chr3:103392259-103392278	None:intergenic	30.0%
AAACTGAAAAATGACAGTGA+TGG	-	Chr3:103386000-103386019	MsG0380018015.01.T01:intron	30.0%
AAAGTTGCCTCCTTATAATT+AGG	-	Chr3:103390094-103390113	MsG0380018015.01.T01:intron	30.0%
AAATGACAGTGATGGTTAAA+AGG	-	Chr3:103386008-103386027	MsG0380018015.01.T01:intron	30.0%
AAATTAAAAATGGTGGTCTC+AGG	+	Chr3:103388675-103388694	None:intergenic	30.0%
AACAAAAACGAGCAAAAAGA+TGG	+	Chr3:103386964-103386983	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	103384424	103394719	103384424	ID=MsG0380018015.01;Name=MsG0380018015.01
Chr3	mRNA	103384424	103394719	103384424	ID=MsG0380018015.01.T01;Parent=MsG0380018015.01;Name=MsG0380018015.01.T01;_AED=0.38;_eAED=0.41;_QI=0\|0.47\|0.36\|1\|0.57\|0.54\|22\|0\|892
Chr3	exon	103394391	103394719	103394391	ID=MsG0380018015.01.T01:exon:18477;Parent=MsG0380018015.01.T01
Chr3	exon	103394228	103394325	103394228	ID=MsG0380018015.01.T01:exon:18476;Parent=MsG0380018015.01.T01
Chr3	exon	103393666	103393718	103393666	ID=MsG0380018015.01.T01:exon:18475;Parent=MsG0380018015.01.T01
Chr3	exon	103392366	103392431	103392366	ID=MsG0380018015.01.T01:exon:18474;Parent=MsG0380018015.01.T01
Chr3	exon	103391992	103392072	103391992	ID=MsG0380018015.01.T01:exon:18473;Parent=MsG0380018015.01.T01
Chr3	exon	103391749	103391808	103391749	ID=MsG0380018015.01.T01:exon:18472;Parent=MsG0380018015.01.T01
Chr3	exon	103391299	103391377	103391299	ID=MsG0380018015.01.T01:exon:18471;Parent=MsG0380018015.01.T01
Chr3	exon	103391110	103391177	103391110	ID=MsG0380018015.01.T01:exon:18470;Parent=MsG0380018015.01.T01
Chr3	exon	103390735	103390791	103390735	ID=MsG0380018015.01.T01:exon:18469;Parent=MsG0380018015.01.T01
Chr3	exon	103390533	103390637	103390533	ID=MsG0380018015.01.T01:exon:18468;Parent=MsG0380018015.01.T01
Chr3	exon	103389995	103390103	103389995	ID=MsG0380018015.01.T01:exon:18467;Parent=MsG0380018015.01.T01
Chr3	exon	103389661	103389878	103389661	ID=MsG0380018015.01.T01:exon:18466;Parent=MsG0380018015.01.T01
Chr3	exon	103389439	103389570	103389439	ID=MsG0380018015.01.T01:exon:18465;Parent=MsG0380018015.01.T01
Chr3	exon	103389153	103389335	103389153	ID=MsG0380018015.01.T01:exon:18464;Parent=MsG0380018015.01.T01
Chr3	exon	103388113	103388313	103388113	ID=MsG0380018015.01.T01:exon:18463;Parent=MsG0380018015.01.T01
Chr3	exon	103387471	103387623	103387471	ID=MsG0380018015.01.T01:exon:18462;Parent=MsG0380018015.01.T01
Chr3	exon	103386780	103386827	103386780	ID=MsG0380018015.01.T01:exon:18461;Parent=MsG0380018015.01.T01
Chr3	exon	103386493	103386636	103386493	ID=MsG0380018015.01.T01:exon:18460;Parent=MsG0380018015.01.T01
Chr3	exon	103385679	103385863	103385679	ID=MsG0380018015.01.T01:exon:18459;Parent=MsG0380018015.01.T01
Chr3	exon	103385339	103385468	103385339	ID=MsG0380018015.01.T01:exon:18458;Parent=MsG0380018015.01.T01
Chr3	exon	103384666	103384740	103384666	ID=MsG0380018015.01.T01:exon:18457;Parent=MsG0380018015.01.T01
Chr3	exon	103384424	103384528	103384424	ID=MsG0380018015.01.T01:exon:18456;Parent=MsG0380018015.01.T01
Chr3	CDS	103394391	103394719	103394391	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103394228	103394325	103394228	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103393666	103393718	103393666	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103392366	103392431	103392366	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103391992	103392072	103391992	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103391749	103391808	103391749	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103391299	103391377	103391299	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103391110	103391177	103391110	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103390735	103390791	103390735	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103390533	103390637	103390533	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103389995	103390103	103389995	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103389661	103389878	103389661	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103389439	103389570	103389439	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103389153	103389335	103389153	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103388113	103388313	103388113	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103387471	103387623	103387471	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103386780	103386827	103386780	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103386493	103386636	103386493	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103385679	103385863	103385679	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103385339	103385468	103385339	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103384666	103384740	103384666	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01
Chr3	CDS	103384424	103384528	103384424	ID=MsG0380018015.01.T01:cds;Parent=MsG0380018015.01.T01

Gene Sequence

>MsG0380018015.01.T01

ATGGCGTCTTCTTCTCGAGCACGAAGTAGCTCACCGTTTTCTCACCGGAAACCATCAACACCTTACTCTTCAACTTCATCCTCTTCTTCTCTCACTAACGGAAGAATAATACCTCGTTCTTCATCTTCCACTACGTCCTCGTTTTTCAACGCTCGATCCACCACTCCGAATCGCGGTCGGAGCGAGTCAACGTGTTACGGAGGTTCGCGCGGTGGTTATAGAGATCGTTCTCCGGTTGCGTTTGGTGGCGAGGAGCTGAGTGTGGATCCTGTTGAGACGTCCACGTCAGCCGATAGCATTTCTGTTACGATTCGGTTTCGTCCGTTAAGTGAAAGGGAATATAATAAAGGTGATGAAATCGCGTGGTATGCTGATGGTGATAAGATTGTTAGAAATGAGTATAATCCAGCAACTGCTTATGCATTTGGAACTGTATTTGCCTATGGTGTGACGAGTAGTGGAAAGACACATACTATGCATGGTGATCAAAATTCTCCCGGTATAATACCATTAGCAATAAAGGATGTTTTCAGCATGATCCAAGATCTATTTCTAATTGAACTTTTGCTGCAGACTCCAGGAAGAGAATTCTTACTCCGAGTGTCATATCTAGAAATATACAACGAGGTGATAAATGATTTGCTTGACCCAACTGGTCAAAATTTACGAGTCAGAGAAGATTCACAGGGAACTTATGTTGAGGGTATAAAGGAAGAAGTTGTTCTATCTCCTGGACACGCCCTTTCTTTTATTGCTGCTGGAGAAGAGCATCGCCATGTCGGATCAAATAATTTTAATCTTTTTAGCAGCAGGAGTCACACAATATTCACACTGATGATTGAAAGCAGTGCCCATGGTGATGAGTATGATGGAGTGATCTTCTCCCAGCTTAACTTGATTGATTTGGCTGGATCTGAAAGTTCGAAAACTGAAACAACTGGATTAAGAAGAAAAGAAGGATCTTACATCAACAAAAGTCTTCTGACACTTGGAACAGTTATAGGAAAGTTGAGTGAGGGAAAGGCATCTCATGTTCCGTATCGAGATTCAAAGCTAACCCGCCTCTTGCAATCTTCTCTTAGTGGGCATGGCCATGTTTCAGTAAGCATTACTATAGCTATTCAGTTTCTCATGTATGGATTTATTGGAACAGAATGTGTTAGGGTGTCTGCACATGAGTTTCTTATTAACATTTGCTGTAACATGAAAATTTCAGCTTATATGCACTATAACTCCAGCGTCCAGCAAATGGAGGAAACTCATAATACTTTGAAGTTTGCCAGCAGGGCGAAACGAGTAGAAATATATGCGTCGCGAAATAAGATTATTGATGAAAAGTCTCTAATTAAGAAATACCAGAGAGAAATTTCTGTTCTAAAACTAGAACTTGATCAAGTAAAGAAGGGAATGCTAGTTGGTGTCAGTCATGAGGAGATTATGACCTTAAAGCAGAAGTTGGAAGAAGGTCAAGTGAAAATGCAATCAAGATTGGAAGAAGAGGAAGATGCCAAGGCTGCTCTTATGAGTAGGATTCAGAGGCTAACCAAACTCATTCTGGTTTCCTCAAAGAATACAATTCCTGGATATCTTACTGATGTTCCCAACCACCAGCGAAGTCACTCTTTTGGCGAGGAAGAAAAACTTGATGCATTTCGTGATGGTATGTTGGTTGAAAATGAGAGTAAAAAAGACGCCTCTGCAGTGTCATCACACCTGTTTCATGATGGTAGACATAAAAGATCATCAAGTAGATGGAACGATGAATTCTCCCCAACTAGCAGTAGTGTTACTGAATCAACACAAGCTGGTGAACTGATAAGCAAAGCAAAACTGGCATCAGGTGGGGTTTCAATATCCGATCAGATGGATCTTCTTGTTGAGCAAGTCAAGATGCTGGCTGGAGACATTGCCTTCAGTACCAGCACTCTGAAGCGCTTGATTGAGCAGTCTGTAAATGACCCTGATGGATCCAAAAGTCAGGTATTCTGCCTCATTAAATCTGCAGACAACCGTGTCCTTCAGGAGCAGCTAAATGATAAGTGTTCTGAAAACAGAGAGTTGCAAGAAAAGTTGAAGCAACTGGAGCAGCAACTAACAGCAAGTTCTAGTGGTACATCATTATCTTCTGAACAATGTGCATCCGGAGAACACATTAATGAGTTAAAAAAGAAGATACAATCCCAGGAAATTGAAAATGAAAAACTTAAGCTAGAGCAAGTTCACTTGTCAGAAGACAATAGTGGGTTGCGTGTCCAGAATCAGAAACTATCGGAGGAAGCTTCTTATGCCAAAGAATTGGCCTCTGCTGCTGCTGTAGAACTGAAGAATTTGGCTGGGGAAGTGACAAAACTTTCATTGCAAAATGCAAAGAAACAGCGAGAGGCTGCTCTGGAGTCTGCGTTGTCTGAGAAAGAATTTGTGGAAGAGGAGTTCAGGAAAAAGGCTGAAGAAGCCAAGAAAAGAGAAGAAGCCTTGGAGAACGATTTAGCTAATATGTGGGCACGTATGCAAGAGATGAAGGAAAAAGAACTCAAGTACCTTGGAAACGGTGATGCCAATTCCCATGTTTGTAAACTCTTGTTTGCTCTTGCAGTGTGTAAATCTTGTTCACTAGCTTGTTCTGAGTGTCCTATATGTCGCACAAATATTACAGATAGACTTTTTGCTTTTACATCTTGA

Protein sequence

>MsG0380018015.01.T01

MASSSRARSSSPFSHRKPSTPYSSTSSSSSLTNGRIIPRSSSSTTSSFFNARSTTPNRGRSESTCYGGSRGGYRDRSPVAFGGEELSVDPVETSTSADSISVTIRFRPLSEREYNKGDEIAWYADGDKIVRNEYNPATAYAFGTVFAYGVTSSGKTHTMHGDQNSPGIIPLAIKDVFSMIQDLFLIELLLQTPGREFLLRVSYLEIYNEVINDLLDPTGQNLRVREDSQGTYVEGIKEEVVLSPGHALSFIAAGEEHRHVGSNNFNLFSSRSHTIFTLMIESSAHGDEYDGVIFSQLNLIDLAGSESSKTETTGLRRKEGSYINKSLLTLGTVIGKLSEGKASHVPYRDSKLTRLLQSSLSGHGHVSVSITIAIQFLMYGFIGTECVRVSAHEFLINICCNMKISAYMHYNSSVQQMEETHNTLKFASRAKRVEIYASRNKIIDEKSLIKKYQREISVLKLELDQVKKGMLVGVSHEEIMTLKQKLEEGQVKMQSRLEEEEDAKAALMSRIQRLTKLILVSSKNTIPGYLTDVPNHQRSHSFGEEEKLDAFRDGMLVENESKKDASAVSSHLFHDGRHKRSSSRWNDEFSPTSSSVTESTQAGELISKAKLASGGVSISDQMDLLVEQVKMLAGDIAFSTSTLKRLIEQSVNDPDGSKSQVFCLIKSADNRVLQEQLNDKCSENRELQEKLKQLEQQLTASSSGTSLSSEQCASGEHINELKKKIQSQEIENEKLKLEQVHLSEDNSGLRVQNQKLSEEASYAKELASAAAVELKNLAGEVTKLSLQNAKKQREAALESALSEKEFVEEEFRKKAEEAKKREEALENDLANMWARMQEMKEKELKYLGNGDANSHVCKLLFALAVCKSCSLACSECPICRTNITDRLFAFTS*