AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001311.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001311.01.T01 MTR_1g035300 98.182 55 1 0 12 66 1 55 6.99e-34 110
MsG0180001311.01.T01 MTR_1g094115 61.667 60 19 1 12 67 1 60 2.21e-13 63.5
MsG0180001311.01.T01 MTR_1g094115 64.286 56 16 1 12 63 1 56 4.84e-12 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 7 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCATCATCTACACCTTTCT+TGG 0.248087 1:-19237625 MsG0180001311.01.T01:CDS
AGGTACTTGCATGGTGTTCC+AGG 0.328858 1:+19237492 None:intergenic
TCTTGTCTGATTTCCAAGAA+AGG 0.471370 1:+19237612 None:intergenic
AAATAATGAAGAATGCATAA+AGG 0.481712 1:+19237472 None:intergenic
AATGCATAAAGGTACTTGCA+TGG 0.607082 1:+19237483 None:intergenic
TTTGCATCAAGCAGAAAACA+TGG 0.629077 1:-19237655 MsG0180001311.01.T01:CDS
AGCAGTTGATGAAGCATCTG+TGG 0.636040 1:+19237554 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTACCAAAAATTATTA+TGG + Chr1:19237572-19237591 None:intergenic 10.0%
!! CTTGGAAAATAAATAAAAAA+AGG + Chr1:19237926-19237945 None:intergenic 15.0%
!! AAATAATGAAGAATGCATAA+AGG + Chr1:19238235-19238254 None:intergenic 20.0%
!! TTACCAAAAATTATTATGGA+GGG + Chr1:19237568-19237587 None:intergenic 20.0%
!! TTTACCAAAAATTATTATGG+AGG + Chr1:19237569-19237588 None:intergenic 20.0%
! AAAAAAAGAAGAAATCAACC+TGG - Chr1:19238194-19238213 MsG0180001311.01.T01:intron 25.0%
! AGAAAAACATCAAATGATTG+AGG - Chr1:19237705-19237724 MsG0180001311.01.T01:intron 25.0%
! TTGCAAATGTATAGTATCAA+AGG - Chr1:19237602-19237621 MsG0180001311.01.T01:CDS 25.0%
!! GTTTTCTTCTCTTTTATCTA+AGG - Chr1:19237772-19237791 MsG0180001311.01.T01:intron 25.0%
!! TTACCCTCCATAATAATTTT+TGG - Chr1:19237562-19237581 MsG0180001311.01.T01:CDS 25.0%
!!! TGATTTCTTCTTTTTTTCTG+AGG + Chr1:19238191-19238210 None:intergenic 25.0%
AAGAGATATGGGTATTTGTA+TGG - Chr1:19237649-19237668 MsG0180001311.01.T01:CDS 30.0%
AGAGATATGGGTATTTGTAT+GGG - Chr1:19237650-19237669 MsG0180001311.01.T01:CDS 30.0%
AGTGTAAAGAGAAAGAGATA+TGG - Chr1:19237637-19237656 MsG0180001311.01.T01:CDS 30.0%
CCAAGAAGAATTTGTATCTA+AGG + Chr1:19237811-19237830 None:intergenic 30.0%
CCTTAGATACAAATTCTTCT+TGG - Chr1:19237808-19237827 MsG0180001311.01.T01:intron 30.0%
GTGTAAAGAGAAAGAGATAT+GGG - Chr1:19237638-19237657 MsG0180001311.01.T01:CDS 30.0%
TGATTGAGGAATAAAAAGAG+AGG - Chr1:19237719-19237738 MsG0180001311.01.T01:intron 30.0%
!!! AAATTCTTCTTGGTGTTTTC+TGG - Chr1:19237818-19237837 MsG0180001311.01.T01:intron 30.0%
!!! AATTCTTCTTGGTGTTTTCT+GGG - Chr1:19237819-19237838 MsG0180001311.01.T01:intron 30.0%
!!! CTTTTTTTCTGAGGAACTAA+AGG + Chr1:19238182-19238201 None:intergenic 30.0%
CAAGAAAGCAAGGAAAATTC+AGG - Chr1:19237871-19237890 MsG0180001311.01.T01:intron 35.0%
TCTTGTCTGATTTCCAAGAA+AGG + Chr1:19238095-19238114 None:intergenic 35.0%
TTCAAAGAGACAAGAAAGCA+AGG - Chr1:19237861-19237880 MsG0180001311.01.T01:intron 35.0%
TTCATCATCTACACCTTTCT+TGG - Chr1:19238079-19238098 MsG0180001311.01.T01:intron 35.0%
TTTGCATCAAGCAGAAAACA+TGG - Chr1:19238049-19238068 MsG0180001311.01.T01:intron 35.0%
!! AATGCATAAAGGTACTTGCA+TGG + Chr1:19238224-19238243 None:intergenic 35.0%
GATATGGGTATTTGTATGGG+TGG - Chr1:19237653-19237672 MsG0180001311.01.T01:CDS 40.0%
TTCCAAGAGACAAGAAAGCA+TGG - Chr1:19237939-19237958 MsG0180001311.01.T01:intron 40.0%
TTCCATGCTTTCTTGTCTCT+TGG + Chr1:19237944-19237963 None:intergenic 40.0%
!!! ACAGGCTACTGTGGTTTTTT+GGG + Chr1:19237505-19237524 None:intergenic 40.0%
!! AGCAGTTGATGAAGCATCTG+TGG + Chr1:19238153-19238172 None:intergenic 45.0%
!!! CACAGGCTACTGTGGTTTTT+TGG + Chr1:19237506-19237525 None:intergenic 45.0%
!!! CAGGCTACTGTGGTTTTTTG+GGG + Chr1:19237504-19237523 None:intergenic 45.0%
AGAGTGTAAGGCCTACACAC+AGG - Chr1:19237469-19237488 MsG0180001311.01.T01:CDS 50.0%
AGGTACTTGCATGGTGTTCC+AGG + Chr1:19238215-19238234 None:intergenic 50.0%
GCAACTCTTGAACAGCACAC+AGG + Chr1:19237523-19237542 None:intergenic 50.0%
TGTAAGGCCTACACACAGGT+CGG - Chr1:19237473-19237492 MsG0180001311.01.T01:CDS 50.0%
GAACAGCACACAGGCTACTG+TGG + Chr1:19237514-19237533 None:intergenic 55.0%
GGCCACACCGACCTGTGTGT+AGG + Chr1:19237483-19237502 None:intergenic 65.0%
GGCCTACACACAGGTCGGTG+TGG - Chr1:19237478-19237497 MsG0180001311.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 19237461 19238265 19237461 ID=MsG0180001311.01;Name=MsG0180001311.01
Chr1 mRNA 19237461 19238265 19237461 ID=MsG0180001311.01.T01;Parent=MsG0180001311.01;Name=MsG0180001311.01.T01;_AED=0.50;_eAED=0.66;_QI=0|0|0|1|0|0|2|0|76
Chr1 exon 19238253 19238265 19238253 ID=MsG0180001311.01.T01:exon:6638;Parent=MsG0180001311.01.T01
Chr1 exon 19237461 19237678 19237461 ID=MsG0180001311.01.T01:exon:6637;Parent=MsG0180001311.01.T01
Chr1 CDS 19238253 19238265 19238253 ID=MsG0180001311.01.T01:cds;Parent=MsG0180001311.01.T01
Chr1 CDS 19237461 19237678 19237461 ID=MsG0180001311.01.T01:cds;Parent=MsG0180001311.01.T01
Gene Sequence

>MsG0180001311.01.T01

ATGAAACAAGAGTATTTGCATCAAGCAGAAAACATGGCTGCTGCTTCATCATCTACACCTTTCTTGGAAATCAGACAAGAAAATCAAAGTCAGATTACACAACAACATCAATCTTCCACAGATGCTTCATCAACTGCTGCTCAACCTTTAGTTCCTCAGAAAAAAAGAAGAAATCAACCTGGAACACCATGCAAGTACCTTTATGCATTCTTCATTATTTCAATACTTTAA

Protein sequence

>MsG0180001311.01.T01

MKQEYLHQAENMAAASSSTPFLEIRQENQSQITQQHQSSTDASSTAAQPLVPQKKRRNQPGTPCKYLYAFFIISIL*