AlfalfaGEDB | MsG0380015804.01

M. sativa cultivar ZhongmuNo.1 / MsG0380015804.01

KEGG: K24265
GO: GO:0004594 GO:0005524 GO:0015937
PFAM: PF01937 PF03630

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015804.01.T01	RYQ85717.1	84.535	860	96	6	11	850	32	874	0	1415

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015804.01.T01	Q8L5Y9	78.875	871	113	7	25	850	29	873	0	1353

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015804.01.T01	G7J5F4	91.919	891	6	3	1	850	1	866	0.0	1645

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000100.01	MsG0380015804.01	0.805265	1.450887e-49	7.641628e-47
MsG0180000125.01	MsG0380015804.01	0.816236	6.135071e-52	4.323312e-49
MsG0180000646.01	MsG0380015804.01	0.804103	2.536800e-49	1.296772e-46
MsG0180000647.01	MsG0380015804.01	0.802041	6.771173e-49	3.283429e-46
MsG0180000854.01	MsG0380015804.01	0.821155	4.693400e-53	3.788434e-50
MsG0180001062.01	MsG0380015804.01	0.810192	1.302297e-50	7.799053e-48
MsG0180001311.01	MsG0380015804.01	0.829708	4.425118e-55	4.565319e-52
MsG0180001759.01	MsG0380015804.01	0.801430	9.036973e-49	4.314811e-46
MsG0180001937.01	MsG0380015804.01	0.801871	7.339715e-49	3.543935e-46
MsG0180004385.01	MsG0380015804.01	0.806811	6.862996e-50	3.760898e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015804.01.T01	MTR_3g077740	91.919	891	6	3	1	850	1	866	0.0	1645
MsG0380015804.01.T01	MTR_8g064850	54.878	410	135	4	44	412	1	401	6.00e-142	426
MsG0380015804.01.T01	MTR_8g064850	55.014	349	107	4	44	351	1	340	2.56e-118	362
MsG0380015804.01.T01	MTR_1g076720	36.043	369	189	10	503	854	11	349	2.63e-60	209
MsG0380015804.01.T01	MTR_6g060550	35.870	368	190	11	503	854	10	347	1.70e-59	207
MsG0380015804.01.T01	MTR_1g076730	35.695	367	193	9	497	849	158	495	1.01e-56	204
MsG0380015804.01.T01	MTR_6g060550	33.721	344	185	10	503	833	51	364	3.27e-47	173
MsG0380015804.01.T01	MTR_6g060550	33.724	341	183	10	503	830	10	320	4.33e-47	171

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380015804.01.T01	AT4G32180	78.875	871	113	7	25	850	29	873	0.0	1353
MsG0380015804.01.T01	AT4G32180	78.718	780	96	6	25	760	29	782	0.0	1224
MsG0380015804.01.T01	AT4G32180	78.718	780	96	6	25	760	29	782	0.0	1224
MsG0380015804.01.T01	AT1G60440	54.684	395	115	7	65	417	3	375	2.09e-115	357
MsG0380015804.01.T01	AT1G60440	54.545	352	134	7	71	417	6	336	1.10e-100	317
MsG0380015804.01.T01	AT1G60440	60.544	294	74	3	165	417	6	298	3.03e-97	306
MsG0380015804.01.T01	AT2G17320	37.609	343	182	9	509	849	21	333	3.51e-63	217
MsG0380015804.01.T01	AT2G17320	37.609	343	182	9	509	849	21	333	4.03e-63	216
MsG0380015804.01.T01	AT4G35360	38.824	340	173	10	520	854	38	347	1.37e-62	216
MsG0380015804.01.T01	AT4G35360	38.824	340	173	10	520	854	38	347	1.37e-62	216

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Find 218 sgRNAs with CRISPR-Local

Find 695 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATTGAAGGAGCGATT+AGG	0.380222	3:+75250728	MsG0380015804.01.T01:CDS
AAAAGCATCACCCCTTCTTT+TGG	0.318392	3:-75256082	None:intergenic
AAACATTGAAGGAGCGATTA+GGG	0.351661	3:+75250729	MsG0380015804.01.T01:CDS
AAAGAGAGAATGTTAGGGAC+CGG	0.506027	3:+75257919	MsG0380015804.01.T01:CDS
AAATGAGGGAAGAGTGCTTG+AGG	0.549228	3:+75256908	MsG0380015804.01.T01:CDS
AACAACAACATGGCTCCTAC+AGG	0.525280	3:+75249617	MsG0380015804.01.T01:CDS
AACAGTAAAGCTCTTCTATG+AGG	0.474599	3:-75257958	None:intergenic
AACATCCAATTATCTTGAGC+AGG	0.503521	3:-75253649	None:intergenic
AACGGAAACACTTCCAAAGT+TGG	0.505796	3:-75254535	None:intergenic
AACTCACTTCAGTTCCACGA+CGG	0.704303	3:-75258041	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATAGGATCATGGTGTT+TGG	-	Chr3:75256363-75256382	None:intergenic	30.0%
AAATGCTCTTTACCTTACAA+GGG	-	Chr3:75251779-75251798	None:intergenic	30.0%
AAATGTCCATCATATAGAGA+AGG	+	Chr3:75252661-75252680	MsG0380015804.01.T01:intron	30.0%
AAGATTTATAGTGCGTTTCA+TGG	+	Chr3:75256283-75256302	MsG0380015804.01.T01:intron	30.0%
AATAAATACAGTTTGGTTGG+TGG	+	Chr3:75249498-75249517	MsG0380015804.01.T01:five_prime_UTR	30.0%
AATACATTATGTATGTCCAG+AGG	-	Chr3:75255699-75255718	None:intergenic	30.0%
AATGTATGACAAAAGCTAAC+AGG	+	Chr3:75252303-75252322	MsG0380015804.01.T01:intron	30.0%
AATGTCCATCATATAGAGAA+GGG	+	Chr3:75252662-75252681	MsG0380015804.01.T01:intron	30.0%
AATTATCTTGAGCAGGTAAT+CGG	-	Chr3:75253645-75253664	None:intergenic	30.0%
ACAAGAATATGAGATAGTGA+AGG	-	Chr3:75259279-75259298	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	75249069	75260059	75249069	ID=MsG0380015804.01;Name=MsG0380015804.01
Chr3	mRNA	75249069	75260059	75249069	ID=MsG0380015804.01.T01;Parent=MsG0380015804.01;Name=MsG0380015804.01.T01;_AED=0.40;_eAED=0.42;_QI=134\|0.9\|0.90\|1\|0.95\|0.90\|21\|0\|857
Chr3	exon	75249069	75249127	75249069	ID=MsG0380015804.01.T01:exon:24620;Parent=MsG0380015804.01.T01
Chr3	exon	75249470	75249782	75249470	ID=MsG0380015804.01.T01:exon:24621;Parent=MsG0380015804.01.T01
Chr3	exon	75250663	75250872	75250663	ID=MsG0380015804.01.T01:exon:24622;Parent=MsG0380015804.01.T01
Chr3	exon	75251026	75251078	75251026	ID=MsG0380015804.01.T01:exon:24623;Parent=MsG0380015804.01.T01
Chr3	exon	75251463	75251579	75251463	ID=MsG0380015804.01.T01:exon:24624;Parent=MsG0380015804.01.T01
Chr3	exon	75252355	75252449	75252355	ID=MsG0380015804.01.T01:exon:24625;Parent=MsG0380015804.01.T01
Chr3	exon	75252534	75252627	75252534	ID=MsG0380015804.01.T01:exon:24626;Parent=MsG0380015804.01.T01
Chr3	exon	75252788	75252919	75252788	ID=MsG0380015804.01.T01:exon:24627;Parent=MsG0380015804.01.T01
Chr3	exon	75253202	75253314	75253202	ID=MsG0380015804.01.T01:exon:24628;Parent=MsG0380015804.01.T01
Chr3	exon	75253415	75253682	75253415	ID=MsG0380015804.01.T01:exon:24629;Parent=MsG0380015804.01.T01
Chr3	exon	75254364	75254575	75254364	ID=MsG0380015804.01.T01:exon:24630;Parent=MsG0380015804.01.T01
Chr3	exon	75255011	75255055	75255011	ID=MsG0380015804.01.T01:exon:24631;Parent=MsG0380015804.01.T01
Chr3	exon	75255146	75255194	75255146	ID=MsG0380015804.01.T01:exon:24632;Parent=MsG0380015804.01.T01
Chr3	exon	75256051	75256133	75256051	ID=MsG0380015804.01.T01:exon:24633;Parent=MsG0380015804.01.T01
Chr3	exon	75256855	75256971	75256855	ID=MsG0380015804.01.T01:exon:24634;Parent=MsG0380015804.01.T01
Chr3	exon	75257078	75257141	75257078	ID=MsG0380015804.01.T01:exon:24635;Parent=MsG0380015804.01.T01
Chr3	exon	75257650	75257796	75257650	ID=MsG0380015804.01.T01:exon:24636;Parent=MsG0380015804.01.T01
Chr3	exon	75257898	75258056	75257898	ID=MsG0380015804.01.T01:exon:24637;Parent=MsG0380015804.01.T01
Chr3	exon	75258648	75258731	75258648	ID=MsG0380015804.01.T01:exon:24638;Parent=MsG0380015804.01.T01
Chr3	exon	75259670	75259861	75259670	ID=MsG0380015804.01.T01:exon:24639;Parent=MsG0380015804.01.T01
Chr3	exon	75259958	75260059	75259958	ID=MsG0380015804.01.T01:exon:24640;Parent=MsG0380015804.01.T01
Chr3	five_prime_UTR	75249069	75249127	75249069	ID=MsG0380015804.01.T01:five_prime_utr;Parent=MsG0380015804.01.T01
Chr3	five_prime_UTR	75249470	75249544	75249470	ID=MsG0380015804.01.T01:five_prime_utr;Parent=MsG0380015804.01.T01
Chr3	CDS	75249545	75249782	75249545	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75250663	75250872	75250663	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75251026	75251078	75251026	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75251463	75251579	75251463	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75252355	75252449	75252355	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75252534	75252627	75252534	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75252788	75252919	75252788	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75253202	75253314	75253202	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75253415	75253682	75253415	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75254364	75254575	75254364	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75255011	75255055	75255011	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75255146	75255194	75255146	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75256051	75256133	75256051	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75256855	75256971	75256855	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75257078	75257141	75257078	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75257650	75257796	75257650	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75257898	75258056	75257898	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75258648	75258731	75258648	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75259670	75259861	75259670	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01
Chr3	CDS	75259958	75260059	75259958	ID=MsG0380015804.01.T01:cds;Parent=MsG0380015804.01.T01

Gene Sequence

>MsG0380015804.01.T01

ATGGCTGATTTGGTTGAAGACGAACAACAACAACAACAAGTTCTTGGTATTGACGAATCTGAGGGACACAACAACAACAACATGGCTCCTACAGGCAATCCAATACATAGATCAAGTTCAAGGCCTCAACTTGATGTTAGCAAAGCTGAAATTCAATCTAATGTTGAGGACAAGTATCCCACCATTTTGTTGCCTAATCAATCTGATGATTTGTCTCATCTCGCTCTTGATATTGGAGGATCTCTGATAAAGTTGGTGTACTTTTCAAGACACGAAGGCCAATCAGACGATGATAAAAGGAAGAAAACATTGAAGGAGCGATTAGGGCTTTCTAATGGTAACAGGAGAAGCTTTCCTATTCTTGGTGGAAGGCTTCACTTTGTCAAATTTGAAACGGGAAAGATCAACGAATGCTTAGATTTCATTCATTCAAAGCAGCTTCACCGTGGGTTGGAGTCACGTTACTCAGATACTGAGGCTGATCGAAATGCCATAATTAAGGCTACTGGAGGTGGAGCATACAAGTTTACCGACCTTTTCAAAGAAAGACTTGGTGTTAGTCTTGACAAAGAAGATGAAATGAATTGTCTTGTGGCAGGAGCAAATTTCTTGCTTAAGGTTGATGGGGATGGGAAGTTTGAGAGGGTTAGTGGGACAAATGTTGGTGGTGGTACTTATTGGGGACTGGGAAGACTGTTAACAAAGTGCAAGAGCTTTGATGAGTTGCTTGAGCTGAGCCAGAAAGGAGATAATAGAGCTATCGACATGCTTGTTGGGGACATTTATGGTGGTTTGGATTATTCTAAGATTGGCCTATCAGCTTCCACTATTGCTTCAAGTTTTGGAAAAACTATATCAGAAAAAAAGGAACTTGAAGACTATAGACCTGAAGACATATCACTCTCTCTTCTACGGATGATTTCATACAACATCGGTCAGATAGCTTACTTAAATGCATTGCGATTTAAGTTGAAGAGAATATTTTTTGGAGGATTTTTTATCAGGGGTCATGCTTATACCATGGACACTCTTTCATTTGCTGTTCAATACTGGTCCAATGGAGAAGCCCAGGCGATGTTTTTGCGACATGAAGGATTTCTGGGAGCTTTAGGTGCATTTATGAGTTACGAAAAGCATGGTTTAGATGACCTCATGGTGCATCAGTTAGTTGAAAGGTTTCCGATGGGTGCTCCATATACTGGTGGCAAGATACATGGCCCACCACTTGGCGATTTGAATGAGAAGGCAAGTGATAGTAATTCAAAATATCTAATTTGTGCCGATTACCTGCTCAAGATAATTGGATGTTCTTGTTCTTTAATTTCGTGGATGGAAAAGTTTTTACAAAAAGGAACAGAAATTACTGCACCTGTGCCGATGACTCCTGTTGCTGGAACTACTGGACTTGGGGGTTTTGAAGTCCCTCTGTCAAAAGGAAGTGCTCTGCGATCTGATGCGAGTGCTCTAAATGTTGGTGTTCTCCATCTAGTACCAACTTTGGAAGTGTTTCCGTTGTTGGCAGACCCAAAATTGTATGAGCCCAATACAATCGATCTTGCAGATCCCAGTGAATTAGAATACTGGCTCACTATTTTGTCAGAGCACTTACCAGATCTTGTGGACAAGGCTGTTGCCAGTGAAGGTGGAACTGATGATGCCAAAAGAAGGGGTGATGCTTTTGCTCGTGCGTTTTCTGCCCACTTGTCAAGGTTGATGGAGGAGCCTTCTGCATACGGCAAGTTAGGCTTGGCCAATCTATTGGAAATGAGGGAAGAGTGCTTGAGGGAATTCCAGTTTGTTGATGCCTATAGAAGTATAAAGCAGAGGGAAAATGAGGCATCACTCGCTGTTTTACCTGACTTGTTTGTAGAACTTGATAGTATGGATGAGGAAACGAGACTGCTTACTCTAATTGAAGGTGTTCTCGCTGCAAACATTTTTGACTGGGGATCCCGTGCATGTGTTGATCTCTATCATAAAGGAACAATTATTGAAATTTACAGAATGAGTCGCAATAAAATGCGCAGACCTTGGCGGGTTGATGATTTTGATCTCTTCAAAGAGAGAATGTTAGGGACCGGGGACAAGAAGAAGCCCCCTCATAGAAGAGCTTTACTGTTTGTTGATAATTCAGGTGCTGACATTGTTCTAGGGATGCTTCCTCTGGCTAGGGAGCTCCTCCGTCGTGGAACTGAAGTTGTTCTCGTTGCAAACTCGCTTCCTGCTTTGAATGATGTGACTGCAATGGAGCTTCCTGATATTGTAGCTGAGGCTGCCAAGCATTGTGACATTCTAAGGCGAGCTGCTGAAGCTGGAGGCTTGCTTGTCGATGCAATGATTAATACAGATAGCTCAAAAGAAAATTCATCTTCGGTCCCCTTGATGGTTGTTGAGAATGGGTGCGGTAGTCCATGTATAGACTTAAGACAGGTCAGCTCGGAATTGGCTGCTGCTGCAAAAGACGCCGATTTGATAATTTTGGAAGGGATGGGTAGATCTCTGCATACTAACCTCTATGCTCAGTTTAAGTGTGATGCTTTGAAGGTAACACATCCTTTGGTTAATGGCTTATGA

Protein sequence

>MsG0380015804.01.T01

MADLVEDEQQQQQVLGIDESEGHNNNNMAPTGNPIHRSSSRPQLDVSKAEIQSNVEDKYPTILLPNQSDDLSHLALDIGGSLIKLVYFSRHEGQSDDDKRKKTLKERLGLSNGNRRSFPILGGRLHFVKFETGKINECLDFIHSKQLHRGLESRYSDTEADRNAIIKATGGGAYKFTDLFKERLGVSLDKEDEMNCLVAGANFLLKVDGDGKFERVSGTNVGGGTYWGLGRLLTKCKSFDELLELSQKGDNRAIDMLVGDIYGGLDYSKIGLSASTIASSFGKTISEKKELEDYRPEDISLSLLRMISYNIGQIAYLNALRFKLKRIFFGGFFIRGHAYTMDTLSFAVQYWSNGEAQAMFLRHEGFLGALGAFMSYEKHGLDDLMVHQLVERFPMGAPYTGGKIHGPPLGDLNEKASDSNSKYLICADYLLKIIGCSCSLISWMEKFLQKGTEITAPVPMTPVAGTTGLGGFEVPLSKGSALRSDASALNVGVLHLVPTLEVFPLLADPKLYEPNTIDLADPSELEYWLTILSEHLPDLVDKAVASEGGTDDAKRRGDAFARAFSAHLSRLMEEPSAYGKLGLANLLEMREECLREFQFVDAYRSIKQRENEASLAVLPDLFVELDSMDEETRLLTLIEGVLAANIFDWGSRACVDLYHKGTIIEIYRMSRNKMRRPWRVDDFDLFKERMLGTGDKKKPPHRRALLFVDNSGADIVLGMLPLARELLRRGTEVVLVANSLPALNDVTAMELPDIVAEAAKHCDILRRAAEAGGLLVDAMINTDSSKENSSSVPLMVVENGCGSPCIDLRQVSSELAAAAKDADLIILEGMGRSLHTNLYAQFKCDALKVTHPLVNGL*