AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021872.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021872.01.T01 MTR_4g478210 91.176 102 5 1 1 102 1 98 3.21e-59 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021872.01.T01 AT1G65000 78.667 75 16 0 27 101 9 83 2.40e-41 131
MsG0480021872.01.T01 AT5G38060 37.662 77 45 1 28 101 58 134 7.44e-11 55.5

Find 20 sgRNAs with CRISPR-Local

Find 284 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTCTTCTCCATCTTCCTTT+CGG 0.362992 4:+65420272 None:intergenic
ACGGCAAGAAGTTGAAGAAT+TGG 0.426200 4:+65420146 None:intergenic
GAGAGCTCAAAGAGGGAGAA+AGG 0.462482 4:-65420296 MsG0480021872.01.T01:exon
GATGGAGAAGAGTGAGAAAG+AGG 0.482147 4:-65420262 MsG0480021872.01.T01:exon
AGAAAGGTCCGAAAGGAAGA+TGG 0.507572 4:-65420280 MsG0480021872.01.T01:exon
GAAGTTGAAGAATTGGAAGC+AGG 0.507959 4:+65420153 None:intergenic
CGAAGAAGGAAGGGTGGAAA+CGG 0.518614 4:+65420127 None:intergenic
GGAGTAGGAGTAGGAGTAGG+AGG 0.520101 4:-65420210 MsG0480021872.01.T01:exon
TCAATATCAAAGGTAGGAGT+AGG 0.522042 4:-65420225 MsG0480021872.01.T01:exon
TCAAATTCAATATCAAAGGT+AGG 0.575533 4:-65420231 MsG0480021872.01.T01:exon
CGGCGACATACGAAGAAGGA+AGG 0.579112 4:+65420117 None:intergenic
TCAAAGGTAGGAGTAGGAGT+AGG 0.580598 4:-65420219 MsG0480021872.01.T01:exon
TGGAGAGGAGAGCTCAAAGA+GGG 0.586393 4:-65420303 MsG0480021872.01.T01:exon
AAATTCAAATTCAATATCAA+AGG 0.588458 4:-65420235 MsG0480021872.01.T01:exon
GTAGGAGTAGGAGTAGGAGT+AGG 0.599717 4:-65420213 MsG0480021872.01.T01:exon
GTGACGGCGACATACGAAGA+AGG 0.612672 4:+65420113 None:intergenic
AAGAGGGAGAAAGGTCCGAA+AGG 0.645014 4:-65420287 MsG0480021872.01.T01:exon
TTGGAGAGGAGAGCTCAAAG+AGG 0.653710 4:-65420304 MsG0480021872.01.T01:exon
GGCGACATACGAAGAAGGAA+GGG 0.660145 4:+65420118 None:intergenic
GACATACGAAGAAGGAAGGG+TGG 0.706048 4:+65420121 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTTTTTTTATTAAACA+AGG + Chr4:65416906-65416925 None:intergenic 10.0%
!!! AATTAAAAAAGGTATAAAAA+TGG + Chr4:65416580-65416599 None:intergenic 10.0%
!! AAAACATTTATTGAAATGTA+AGG + Chr4:65418362-65418381 None:intergenic 15.0%
!! AAATTCAAATTCAATATCAA+AGG - Chr4:65416259-65416278 MsG0480021872.01.T01:exon 15.0%
!! AACAAAAGATAAAATAAAGT+AGG + Chr4:65419375-65419394 None:intergenic 15.0%
!! AATCATATTCATAAAAATAC+AGG + Chr4:65416527-65416546 None:intergenic 15.0%
!! ACAAAAGATAAAATAAAGTA+GGG + Chr4:65419374-65419393 None:intergenic 15.0%
!! ATATAATTAAGAAAAAAACC+CGG - Chr4:65417133-65417152 MsG0480021872.01.T01:intron 15.0%
!! ATCATATTCATAAAAATACA+GGG + Chr4:65416526-65416545 None:intergenic 15.0%
!! TATATTTGTTTATTTATCAG+AGG - Chr4:65417490-65417509 MsG0480021872.01.T01:intron 15.0%
!! TGATAAGTGATAATTAAAAA+AGG + Chr4:65416591-65416610 None:intergenic 15.0%
!! TTCTTATCAAAATTAAAACT+GGG - Chr4:65418491-65418510 MsG0480021872.01.T01:intron 15.0%
!! TTTCTTATCAAAATTAAAAC+TGG - Chr4:65418490-65418509 MsG0480021872.01.T01:intron 15.0%
!!! AAAAACATTTTTGAAATGTA+AGG - Chr4:65419300-65419319 MsG0480021872.01.T01:intron 15.0%
!!! GAAAACAATTTTACTTTATT+GGG - Chr4:65418288-65418307 MsG0480021872.01.T01:intron 15.0%
!!! TAAATTTTCATTGATTTGAA+TGG + Chr4:65416749-65416768 None:intergenic 15.0%
!!! TGAAAACAATTTTACTTTAT+TGG - Chr4:65418287-65418306 MsG0480021872.01.T01:intron 15.0%
!! AAAATAATCGAATAGAAAAG+GGG + Chr4:65416427-65416446 None:intergenic 20.0%
!! AAATTGTTTACAGTTAATAC+AGG + Chr4:65419909-65419928 None:intergenic 20.0%
!! AATCATATCCAATATATTGT+TGG - Chr4:65417192-65417211 MsG0480021872.01.T01:intron 20.0%
!! AGAAAATAATCGAATAGAAA+AGG + Chr4:65416429-65416448 None:intergenic 20.0%
!! AGAATTTGATGAATAAATGA+TGG + Chr4:65417876-65417895 None:intergenic 20.0%
!! ATAGTTTGATTATACTAGTT+TGG - Chr4:65420056-65420075 MsG0480021872.01.T01:intron 20.0%
!! GAAAATAATCGAATAGAAAA+GGG + Chr4:65416428-65416447 None:intergenic 20.0%
!! GGATATAATTGAGAAATTTA+CGG + Chr4:65417669-65417688 None:intergenic 20.0%
!! TCATATAAAGTTTAAACTTG+TGG - Chr4:65419929-65419948 MsG0480021872.01.T01:intron 20.0%
!! TTTGTTTGTGTTAATTATGA+AGG - Chr4:65419558-65419577 MsG0480021872.01.T01:intron 20.0%
!!! AAAATTTCTAGATTAAGTTG+CGG - Chr4:65417087-65417106 MsG0480021872.01.T01:intron 20.0%
!!! AAATATAAGTGTTTTCAACA+TGG + Chr4:65417477-65417496 None:intergenic 20.0%
!!! ACACTAATTTGATAACAATT+TGG + Chr4:65417692-65417711 None:intergenic 20.0%
!!! ACAGCTAAAATTTTTAAATC+TGG - Chr4:65417961-65417980 MsG0480021872.01.T01:intron 20.0%
!!! ACTAATTTGATAACAATTTG+GGG + Chr4:65417690-65417709 None:intergenic 20.0%
!!! ATCAGCTATTAATTTGAATT+GGG - Chr4:65416607-65416626 MsG0480021872.01.T01:exon 20.0%
!!! ATTGCTATTTTGAAAATTTG+AGG - Chr4:65416829-65416848 MsG0480021872.01.T01:CDS 20.0%
!!! CAACTTAATCTAGAAATTTT+GGG + Chr4:65417087-65417106 None:intergenic 20.0%
!!! CAATTTTTCTAGTTACATTT+GGG - Chr4:65419880-65419899 MsG0480021872.01.T01:intron 20.0%
!!! CACTAATTTGATAACAATTT+GGG + Chr4:65417691-65417710 None:intergenic 20.0%
!!! TACAGATATATTTTGATAGA+GGG + Chr4:65416718-65416737 None:intergenic 20.0%
!!! TATCAGCTATTAATTTGAAT+TGG - Chr4:65416606-65416625 MsG0480021872.01.T01:exon 20.0%
!!! TATTGACTAGTAGAATAAAA+AGG + Chr4:65417586-65417605 None:intergenic 20.0%
!!! TATTGGGTTAAATATGTTTT+TGG - Chr4:65418304-65418323 MsG0480021872.01.T01:intron 20.0%
!!! TCAATTTTTCTAGTTACATT+TGG - Chr4:65419879-65419898 MsG0480021872.01.T01:intron 20.0%
!!! TTAGACATTTCATTTTCTTT+TGG + Chr4:65417942-65417961 None:intergenic 20.0%
!!! TTGCTATTTTGAAAATTTGA+GGG - Chr4:65416830-65416849 MsG0480021872.01.T01:CDS 20.0%
!!! TTTCATTTGCACTTTTATTT+TGG - Chr4:65417246-65417265 MsG0480021872.01.T01:intron 20.0%
! AACTTGTAAACCATGTAAAA+TGG + Chr4:65419770-65419789 None:intergenic 25.0%
! AGAATAAAAAGGAGAAAGTA+AGG + Chr4:65417575-65417594 None:intergenic 25.0%
! ATATCCAATATATTGTTGGA+AGG - Chr4:65417196-65417215 MsG0480021872.01.T01:intron 25.0%
! ATCAAAATTAAAACTGGGTT+TGG - Chr4:65418496-65418515 MsG0480021872.01.T01:intron 25.0%
! ATGAAAAAATGAGTCATACA+TGG + Chr4:65417736-65417755 None:intergenic 25.0%
! ATGCACTATATAAAACAGAT+AGG + Chr4:65416937-65416956 None:intergenic 25.0%
! ATGCTACTATAATTAAGATG+TGG + Chr4:65416484-65416503 None:intergenic 25.0%
! ATGTTCTTAGTCTCAAATAT+AGG - Chr4:65420121-65420140 MsG0480021872.01.T01:exon 25.0%
! ATTACTTCCTAATCATCAAA+CGG + Chr4:65419232-65419251 None:intergenic 25.0%
! CTCAAATATAGGGAAATTAT+TGG - Chr4:65420132-65420151 MsG0480021872.01.T01:exon 25.0%
! GCTTATTATGCAAAAATAGA+CGG + Chr4:65418428-65418447 None:intergenic 25.0%
! GTTAACGGAAGTAAAATATA+GGG + Chr4:65418391-65418410 None:intergenic 25.0%
! TAGCATTAACATTGATCATA+AGG - Chr4:65416498-65416517 MsG0480021872.01.T01:exon 25.0%
! TATAATTGAGAAATTTACGG+AGG + Chr4:65417666-65417685 None:intergenic 25.0%
! TCAAAATTAAAACTGGGTTT+GGG - Chr4:65418497-65418516 MsG0480021872.01.T01:intron 25.0%
! TCAAATTCAATATCAAAGGT+AGG - Chr4:65416263-65416282 MsG0480021872.01.T01:exon 25.0%
! TGATTGATGTTACTCAATTT+GGG - Chr4:65419043-65419062 MsG0480021872.01.T01:intron 25.0%
! TGGTGAAAATGATATTAACA+AGG + Chr4:65419750-65419769 None:intergenic 25.0%
! TGTTAACGGAAGTAAAATAT+AGG + Chr4:65418392-65418411 None:intergenic 25.0%
! TGTTAATTCTCTATTCTAGA+AGG - Chr4:65417801-65417820 MsG0480021872.01.T01:intron 25.0%
! TGTTCTTAGTCTCAAATATA+GGG - Chr4:65420122-65420141 MsG0480021872.01.T01:exon 25.0%
! TTAGTCTATGTTTGTTTATG+CGG - Chr4:65417270-65417289 MsG0480021872.01.T01:intron 25.0%
! TTCATAATGATACTGTGTTA+AGG + Chr4:65417532-65417551 None:intergenic 25.0%
! TTGCATAATTAAGTGACATA+AGG - Chr4:65418036-65418055 MsG0480021872.01.T01:intron 25.0%
!! ACAATTTCGAGTAAAATTGA+TGG + Chr4:65416660-65416679 None:intergenic 25.0%
!! AGTAGGGTTAAATATGTTTT+TGG + Chr4:65419358-65419377 None:intergenic 25.0%
!! ATCATTTTCACCATTTTACA+TGG - Chr4:65419757-65419776 MsG0480021872.01.T01:intron 25.0%
!! GCAACTTAATCTAGAAATTT+TGG + Chr4:65417088-65417107 None:intergenic 25.0%
!! TATCATTTTATGACCTGTTT+GGG - Chr4:65418118-65418137 MsG0480021872.01.T01:intron 25.0%
!! TTATCATTTTATGACCTGTT+TGG - Chr4:65418117-65418136 MsG0480021872.01.T01:intron 25.0%
!! TTATTGACCTTTTTATACAC+CGG - Chr4:65420268-65420287 MsG0480021872.01.T01:exon 25.0%
!! TTTTGTTAGGGTTGAATTTT+GGG - Chr4:65419499-65419518 MsG0480021872.01.T01:intron 25.0%
!! TTTTTGTTAGGGTTGAATTT+TGG - Chr4:65419498-65419517 MsG0480021872.01.T01:intron 25.0%
!!! ATTATGAAGGTATTATGAAC+TGG - Chr4:65419571-65419590 MsG0480021872.01.T01:intron 25.0%
!!! CTACAGATATATTTTGATAG+AGG + Chr4:65416719-65416738 None:intergenic 25.0%
!!! GATAACGGAATAAAAATGTA+GGG - Chr4:65419272-65419291 MsG0480021872.01.T01:intron 25.0%
!!! GTTATTTTCATAAGCTATCT+AGG + Chr4:65418249-65418268 None:intergenic 25.0%
!!! TGATAACGGAATAAAAATGT+AGG - Chr4:65419271-65419290 MsG0480021872.01.T01:intron 25.0%
!!! TGTATGACTCATTTTTTCAT+TGG - Chr4:65417736-65417755 MsG0480021872.01.T01:intron 25.0%
!!! TTAATCTAGAAATTTTGGGT+AGG + Chr4:65417083-65417102 None:intergenic 25.0%
!!! TTACGTCTTTGAAATTGTAA+AGG + Chr4:65416870-65416889 None:intergenic 25.0%
!!! TTATGAAGGTATTATGAACT+GGG - Chr4:65419572-65419591 MsG0480021872.01.T01:intron 25.0%
AAACAAACAATAACCGAACA+TGG + Chr4:65419545-65419564 None:intergenic 30.0%
AAACATCATAACTATACCCA+TGG + Chr4:65418064-65418083 None:intergenic 30.0%
AACAAACAATAACCGAACAT+GGG + Chr4:65419544-65419563 None:intergenic 30.0%
AACTTAAAATAGACAGGACT+TGG + Chr4:65417617-65417636 None:intergenic 30.0%
AATCTATCTATTCCCATGTT+CGG - Chr4:65419529-65419548 MsG0480021872.01.T01:intron 30.0%
AATTAGTCCGGTGTATAAAA+AGG + Chr4:65420278-65420297 None:intergenic 30.0%
AATTGAGTAACATCAATCAC+AGG + Chr4:65419042-65419061 None:intergenic 30.0%
ACAGAAAAACAAAGACCAAT+TGG + Chr4:65420209-65420228 None:intergenic 30.0%
AGTAAGCTGAAAACAGTATA+TGG + Chr4:65418214-65418233 None:intergenic 30.0%
ATGTTTATTGTTCTGCAAAG+GGG - Chr4:65418078-65418097 MsG0480021872.01.T01:intron 30.0%
GATGTTTATTGTTCTGCAAA+GGG - Chr4:65418077-65418096 MsG0480021872.01.T01:intron 30.0%
GGAAACAACTTAAAATAGAC+AGG + Chr4:65417623-65417642 None:intergenic 30.0%
GTATAAAGCAAACACTGTAT+TGG - Chr4:65420304-65420323 MsG0480021872.01.T01:exon 30.0%
GTCATAAAATGATAAGCAAC+AGG + Chr4:65418112-65418131 None:intergenic 30.0%
GTGATTGATGTTACTCAATT+TGG - Chr4:65419042-65419061 MsG0480021872.01.T01:intron 30.0%
TAAAGTTTAAACTTGTGGTG+TGG - Chr4:65419934-65419953 MsG0480021872.01.T01:intron 30.0%
TAGAGTTTACAATGAGTTCA+TGG - Chr4:65417037-65417056 MsG0480021872.01.T01:intron 30.0%
TAGTGTAAGATTAAGATGTC+AGG - Chr4:65417706-65417725 MsG0480021872.01.T01:intron 30.0%
TCAAAGTAGCGCAAAATATT+GGG + Chr4:65418331-65418350 None:intergenic 30.0%
TCAAAGTAGCGTAAAATGTA+GGG - Chr4:65419331-65419350 MsG0480021872.01.T01:intron 30.0%
TGATGTTTATTGTTCTGCAA+AGG - Chr4:65418076-65418095 MsG0480021872.01.T01:intron 30.0%
TTATACTCCTTCAATTAGTC+CGG + Chr4:65420290-65420309 None:intergenic 30.0%
TTCAAAGTAGCGCAAAATAT+TGG + Chr4:65418332-65418351 None:intergenic 30.0%
TTCAAAGTAGCGTAAAATGT+AGG - Chr4:65419330-65419349 MsG0480021872.01.T01:intron 30.0%
TTGACACTACTAAATTGACT+TGG - Chr4:65416963-65416982 MsG0480021872.01.T01:intron 30.0%
TTGACTTAAATAATCTCCTG+TGG - Chr4:65416773-65416792 MsG0480021872.01.T01:exon 30.0%
TTGCTTCTAACGAATTTAGA+AGG - Chr4:65419087-65419106 MsG0480021872.01.T01:intron 30.0%
TTTACTTCCGTTAACACAAA+TGG - Chr4:65418396-65418415 MsG0480021872.01.T01:intron 30.0%
TTTATCAAATTTAGCACCAC+AGG + Chr4:65416792-65416811 None:intergenic 30.0%
! AAAACACTTGTGAGTCTTTT+TGG - Chr4:65418171-65418190 MsG0480021872.01.T01:intron 30.0%
! AAACACTTGTGAGTCTTTTT+GGG - Chr4:65418172-65418191 MsG0480021872.01.T01:intron 30.0%
! GAGCTAGAAACTTTTTGTTA+GGG - Chr4:65419487-65419506 MsG0480021872.01.T01:intron 30.0%
! GATTTTACGGTTGTGAATTT+TGG + Chr4:65418612-65418631 None:intergenic 30.0%
! GTGATAAGGTTACATTTTGT+TGG - Chr4:65417163-65417182 MsG0480021872.01.T01:intron 30.0%
! TGAGCTAGAAACTTTTTGTT+AGG - Chr4:65419486-65419505 MsG0480021872.01.T01:intron 30.0%
! TTTTCAGCTTCTATTTTCCA+CGG - Chr4:65417325-65417344 MsG0480021872.01.T01:intron 30.0%
! TTTTCATAAGCTATCTAGGT+AGG + Chr4:65418245-65418264 None:intergenic 30.0%
!! CATGGTGGAAAATGATTTTA+CGG + Chr4:65418625-65418644 None:intergenic 30.0%
!! GTCTTTGAAATTGTAAAGGT+CGG + Chr4:65416866-65416885 None:intergenic 30.0%
!!! TCATGCGTGAAATTTTTTGT+TGG - Chr4:65417999-65418018 MsG0480021872.01.T01:intron 30.0%
!!! TTGGTTTGCTGAAACATTTT+AGG - Chr4:65420075-65420094 MsG0480021872.01.T01:intron 30.0%
AATTGCATCACATCTCACAT+GGG + Chr4:65419989-65420008 None:intergenic 35.0%
AGATGTGATGCAATTGAGAT+CGG - Chr4:65419994-65420013 MsG0480021872.01.T01:intron 35.0%
ATCATAGAATTTCGCAGCAT+AGG + Chr4:65419612-65419631 None:intergenic 35.0%
ATTAGGAAGTAATTGACAGC+AGG - Chr4:65419239-65419258 MsG0480021872.01.T01:intron 35.0%
ATTATGCAAAAATAGACGGC+AGG + Chr4:65418424-65418443 None:intergenic 35.0%
ATTGTCCAGAGCTAAGTAAA+GGG + Chr4:65419721-65419740 None:intergenic 35.0%
CAAAATGGTTGTATTTGACC+CGG - Chr4:65418521-65418540 MsG0480021872.01.T01:intron 35.0%
CGAAATTCTATGATTGCTGT+GGG - Chr4:65419618-65419637 MsG0480021872.01.T01:intron 35.0%
CGTAAAACATAAGACATCCT+CGG - Chr4:65420163-65420182 MsG0480021872.01.T01:exon 35.0%
CTGTCCTTCCAACAATATAT+TGG + Chr4:65417203-65417222 None:intergenic 35.0%
GAAATTCTATGATTGCTGTG+GGG - Chr4:65419619-65419638 MsG0480021872.01.T01:intron 35.0%
GAGAAAGATGAAGGTCAATT+TGG - Chr4:65419117-65419136 MsG0480021872.01.T01:intron 35.0%
GAGAATTGAAGCGTAGAATT+AGG + Chr4:65416460-65416479 None:intergenic 35.0%
GCAGGGACTATTAATGATAA+CGG - Chr4:65419257-65419276 MsG0480021872.01.T01:intron 35.0%
GGAAAAGATGAGAAAGATGA+AGG - Chr4:65419108-65419127 MsG0480021872.01.T01:intron 35.0%
GGTGGAAAAAAGATGAACAT+GGG + Chr4:65418562-65418581 None:intergenic 35.0%
TAACCTTATCACAGTAAAGC+CGG + Chr4:65417155-65417174 None:intergenic 35.0%
TAAGCCTGATTTAAGTGACA+CGG + Chr4:65417916-65417935 None:intergenic 35.0%
TAAGTGACATAAGGAACCAT+GGG - Chr4:65418045-65418064 MsG0480021872.01.T01:intron 35.0%
TATTCTACTAGTCAATACCG+AGG - Chr4:65417589-65417608 MsG0480021872.01.T01:intron 35.0%
TCAAATATGTCAACCCAAAC+AGG + Chr4:65418134-65418153 None:intergenic 35.0%
TCAATATCAAAGGTAGGAGT+AGG - Chr4:65416269-65416288 MsG0480021872.01.T01:exon 35.0%
TCAGAGGTAATAAACTACCA+AGG - Chr4:65417506-65417525 MsG0480021872.01.T01:intron 35.0%
TCATAGAATTTCGCAGCATA+GGG + Chr4:65419611-65419630 None:intergenic 35.0%
TCTCATTCCAAAAGATGTGA+AGG - Chr4:65419005-65419024 MsG0480021872.01.T01:intron 35.0%
TGAAATGATTGCACTCCAAT+TGG - Chr4:65420191-65420210 MsG0480021872.01.T01:exon 35.0%
TGAGAAATTTACGGAGGTTA+CGG + Chr4:65417660-65417679 None:intergenic 35.0%
TGCTACGTAAGACACAAAAT+GGG - Chr4:65419180-65419199 MsG0480021872.01.T01:intron 35.0%
TGGGTCAATTTCATATGAAC+CGG + Chr4:65418543-65418562 None:intergenic 35.0%
TGGTGGAAAAAAGATGAACA+TGG + Chr4:65418563-65418582 None:intergenic 35.0%
TTAAGTGACATAAGGAACCA+TGG - Chr4:65418044-65418063 MsG0480021872.01.T01:intron 35.0%
TTAGGAAGTAATTGACAGCA+GGG - Chr4:65419240-65419259 MsG0480021872.01.T01:intron 35.0%
TTATACACCGGACTAATTGA+AGG - Chr4:65420280-65420299 MsG0480021872.01.T01:exon 35.0%
TTATGCAAAAATAGACGGCA+GGG + Chr4:65418423-65418442 None:intergenic 35.0%
TTTGAATAGCATGTTGATGC+TGG - Chr4:65417424-65417443 MsG0480021872.01.T01:intron 35.0%
! AAGCATTCCGTTTGATGATT+AGG - Chr4:65419222-65419241 MsG0480021872.01.T01:intron 35.0%
! CGAGGATGTCTTATGTTTTA+CGG + Chr4:65420165-65420184 None:intergenic 35.0%
! TGTCACTTAAATCAGGCTTA+TGG - Chr4:65417916-65417935 MsG0480021872.01.T01:intron 35.0%
! TGTTTTCAACATGGCATCAA+TGG + Chr4:65417468-65417487 None:intergenic 35.0%
!! ACTCACAAGTGTTTTTATGC+TGG + Chr4:65418166-65418185 None:intergenic 35.0%
!! TACGGTTGTGAATTTTGGAA+TGG + Chr4:65418607-65418626 None:intergenic 35.0%
!! TTGACTTGGAGAAATCTCAT+AGG - Chr4:65416977-65416996 MsG0480021872.01.T01:intron 35.0%
!!! AAAAGGGGAGTTACCTTTTT+TGG + Chr4:65416412-65416431 None:intergenic 35.0%
!!! TTCAAAGTCTTTTTTCACCG+TGG + Chr4:65417345-65417364 None:intergenic 35.0%
AACCTTATCACAGTAAAGCC+GGG + Chr4:65417154-65417173 None:intergenic 40.0%
AAGAAGAACAGGAAGAAGAG+AGG - Chr4:65418848-65418867 MsG0480021872.01.T01:intron 40.0%
AAGCAAACAGAATGCTGATG+TGG - Chr4:65418444-65418463 MsG0480021872.01.T01:intron 40.0%
AAGCCTGATTTAAGTGACAC+GGG + Chr4:65417915-65417934 None:intergenic 40.0%
AAGCTATCTAGGTAGGCTTA+TGG + Chr4:65418238-65418257 None:intergenic 40.0%
ACGGCAAGAAGTTGAAGAAT+TGG + Chr4:65416351-65416370 None:intergenic 40.0%
ACTCTTCTCCATCTTCCTTT+CGG + Chr4:65416225-65416244 None:intergenic 40.0%
ACTTGCAGCAAAATTGAGCA+TGG + Chr4:65418583-65418602 None:intergenic 40.0%
ATGATACTGTGTTAAGGCCT+TGG + Chr4:65417526-65417545 None:intergenic 40.0%
ATGGATGATACTTGTCGAAG+AGG + Chr4:65418963-65418982 None:intergenic 40.0%
CAATTGCATCACATCTCACA+TGG + Chr4:65419990-65420009 None:intergenic 40.0%
CTCTCTAAACATGCATCAAG+TGG + Chr4:65417303-65417322 None:intergenic 40.0%
CTGCTACGTAAGACACAAAA+TGG - Chr4:65419179-65419198 MsG0480021872.01.T01:intron 40.0%
GAAGTTGAAGAATTGGAAGC+AGG + Chr4:65416344-65416363 None:intergenic 40.0%
GATACTTGTCGAAGAGGATA+CGG + Chr4:65418957-65418976 None:intergenic 40.0%
GATTGTCCAGAGCTAAGTAA+AGG + Chr4:65419722-65419741 None:intergenic 40.0%
GCAATCATTTCAAAACACCG+AGG + Chr4:65420183-65420202 None:intergenic 40.0%
GCGAAATTCTATGATTGCTG+TGG - Chr4:65419617-65419636 MsG0480021872.01.T01:intron 40.0%
GGGTCAATTTCATATGAACC+GGG + Chr4:65418542-65418561 None:intergenic 40.0%
TCTTCCTGTTCTTCTTGAAG+CGG + Chr4:65418844-65418863 None:intergenic 40.0%
TCTTGAGTACGTGTTTGCTT+TGG + Chr4:65417391-65417410 None:intergenic 40.0%
TGTTTGCTTTGGCGTTTAAG+AGG + Chr4:65417380-65417399 None:intergenic 40.0%
! ATCTTCGTGAGGTTTTGCTT+TGG + Chr4:65418815-65418834 None:intergenic 40.0%
! CATTTTGTGTCTTACGTAGC+AGG + Chr4:65419181-65419200 None:intergenic 40.0%
! CGGGTCAAATACAACCATTT+TGG + Chr4:65418523-65418542 None:intergenic 40.0%
! GCTTTCTCCTTCACATCTTT+TGG + Chr4:65419015-65419034 None:intergenic 40.0%
!! GTTACGGATTTTGACAAGTG+AGG + Chr4:65417644-65417663 None:intergenic 40.0%
AAACTGGGTTTGGGCCAAAA+TGG - Chr4:65418506-65418525 MsG0480021872.01.T01:intron 45.0%
AACCCGGCTTTACTGTGATA+AGG - Chr4:65417149-65417168 MsG0480021872.01.T01:intron 45.0%
AAGACTTTGAACCGTGCGAA+TGG - Chr4:65417354-65417373 MsG0480021872.01.T01:intron 45.0%
AGAAAGGTCCGAAAGGAAGA+TGG - Chr4:65416214-65416233 MsG0480021872.01.T01:exon 45.0%
ATAGACAGGACTTGGAACCT+CGG + Chr4:65417609-65417628 None:intergenic 45.0%
ATGCTTGTGTGGCATAAGAG+TGG + Chr4:65419208-65419227 None:intergenic 45.0%
CATCAAACGGAATGCTTGTG+TGG + Chr4:65419219-65419238 None:intergenic 45.0%
CTTCCTGTTCTTCTTGAAGC+GGG + Chr4:65418843-65418862 None:intergenic 45.0%
GATGGAGAAGAGTGAGAAAG+AGG - Chr4:65416232-65416251 MsG0480021872.01.T01:exon 45.0%
GCAGGGACCATTTGTGTTAA+CGG + Chr4:65418406-65418425 None:intergenic 45.0%
GCGATCTTGTCGATGTAACA+TGG + Chr4:65418982-65419001 None:intergenic 45.0%
TAGAATTTCGCAGCATAGGG+TGG + Chr4:65419608-65419627 None:intergenic 45.0%
TCAAAGGTAGGAGTAGGAGT+AGG - Chr4:65416275-65416294 MsG0480021872.01.T01:exon 45.0%
TCACACCCTTTACTTAGCTC+TGG - Chr4:65419713-65419732 MsG0480021872.01.T01:intron 45.0%
TGCAGCAAAATTGAGCATGG+TGG + Chr4:65418580-65418599 None:intergenic 45.0%
TGTTGGAAGGACAGAACATC+AGG - Chr4:65417209-65417228 MsG0480021872.01.T01:intron 45.0%
! AAGGTATTATGAACTGGGCC+CGG - Chr4:65419577-65419596 MsG0480021872.01.T01:intron 45.0%
! ATGGGAGTTCCGATTTGTGA+TGG + Chr4:65418771-65418790 None:intergenic 45.0%
! TGGGAGTTCCGATTTGTGAT+GGG + Chr4:65418770-65418789 None:intergenic 45.0%
!! AGGTTTTGCTTTGGATGCGA+TGG + Chr4:65418806-65418825 None:intergenic 45.0%
!!! ACCTTTTTTGGTCGTCGTGA+CGG + Chr4:65416400-65416419 None:intergenic 45.0%
!! AAAAAAATAAAAAAATCTAA+TGG + Chr4:65419144-65419163 None:intergenic 5.0%
!! AATTTGATAAAAAAAAAAAA+AGG - Chr4:65416801-65416820 MsG0480021872.01.T01:exon 5.0%
AAGAGGGAGAAAGGTCCGAA+AGG - Chr4:65416207-65416226 MsG0480021872.01.T01:exon 50.0%
ATCACATCTCACATGGGAGG+GGG + Chr4:65419983-65420002 None:intergenic 50.0%
CATCACATCTCACATGGGAG+GGG + Chr4:65419984-65420003 None:intergenic 50.0%
CGAAGAAGGAAGGGTGGAAA+CGG + Chr4:65416370-65416389 None:intergenic 50.0%
CGTTTAAGAGGCCATTCGCA+CGG + Chr4:65417368-65417387 None:intergenic 50.0%
GACATACGAAGAAGGAAGGG+TGG + Chr4:65416376-65416395 None:intergenic 50.0%
GAGAGCTCAAAGAGGGAGAA+AGG - Chr4:65416198-65416217 MsG0480021872.01.T01:exon 50.0%
GCATCACATCTCACATGGGA+GGG + Chr4:65419985-65420004 None:intergenic 50.0%
GGCGACATACGAAGAAGGAA+GGG + Chr4:65416379-65416398 None:intergenic 50.0%
GTAGGAGTAGGAGTAGGAGT+AGG - Chr4:65416281-65416300 MsG0480021872.01.T01:exon 50.0%
TCAGGAGGTCGTAGTAACGA+CGG - Chr4:65418916-65418935 MsG0480021872.01.T01:intron 50.0%
TGAGAGAGCTGAAGAGAGCA+TGG + Chr4:65418643-65418662 None:intergenic 50.0%
TGCATCACATCTCACATGGG+AGG + Chr4:65419986-65420005 None:intergenic 50.0%
TGTCGAAGAGGATACGGTGA+TGG + Chr4:65418951-65418970 None:intergenic 50.0%
! AGTTCCGATTTGTGATGGGG+CGG + Chr4:65418766-65418785 None:intergenic 50.0%
! GGGAGTTCCGATTTGTGATG+GGG + Chr4:65418769-65418788 None:intergenic 50.0%
!! AAAGCATCGCAAGCATCAGG+AGG - Chr4:65418901-65418920 MsG0480021872.01.T01:intron 50.0%
!! GAGCCCGTGTCACTTAAATC+AGG - Chr4:65417909-65417928 MsG0480021872.01.T01:intron 50.0%
ACAAGGGTCGTGTAAGAGGG+AGG + Chr4:65418684-65418703 None:intergenic 55.0%
AGGGACAAGGGTCGTGTAAG+AGG + Chr4:65418688-65418707 None:intergenic 55.0%
CAAGGGTCGTGTAAGAGGGA+GGG + Chr4:65418683-65418702 None:intergenic 55.0%
CAGCATAGGGTGGATCATCC+GGG + Chr4:65419598-65419617 None:intergenic 55.0%
CGACCCGCTTCAAGAAGAAC+AGG - Chr4:65418837-65418856 MsG0480021872.01.T01:intron 55.0%
CGGCGACATACGAAGAAGGA+AGG + Chr4:65416380-65416399 None:intergenic 55.0%
CTATGATTGCTGTGGGGCTG+AGG - Chr4:65419625-65419644 MsG0480021872.01.T01:intron 55.0%
CTTTCCGATACGACCGTGAC+CGG + Chr4:65418885-65418904 None:intergenic 55.0%
GAGGGGGTCAGAGTGATCTA+TGG + Chr4:65419967-65419986 None:intergenic 55.0%
GCAGCATAGGGTGGATCATC+CGG + Chr4:65419599-65419618 None:intergenic 55.0%
GCCGTCACGACGACCAAAAA+AGG - Chr4:65416396-65416415 MsG0480021872.01.T01:exon 55.0%
GGAGTAGGAGTAGGAGTAGG+AGG - Chr4:65416284-65416303 MsG0480021872.01.T01:exon 55.0%
GGGACAAGGGTCGTGTAAGA+GGG + Chr4:65418687-65418706 None:intergenic 55.0%
GTGACGGCGACATACGAAGA+AGG + Chr4:65416384-65416403 None:intergenic 55.0%
TAGTGCAGCCAGATGCCTCA+GGG + Chr4:65419652-65419671 None:intergenic 55.0%
! GAGAGCTGAAGAGAGCATGG+TGG + Chr4:65418640-65418659 None:intergenic 55.0%
! GAGAGTGACGAAGATGGTGG+TGG + Chr4:65418730-65418749 None:intergenic 55.0%
!! CGGAAAGCATCGCAAGCATC+AGG - Chr4:65418898-65418917 MsG0480021872.01.T01:intron 55.0%
!! TGACGAAGATGGTGGTGGTG+CGG + Chr4:65418725-65418744 None:intergenic 55.0%
AAGAGAGGTCACGTGAGCGC+CGG - Chr4:65418863-65418882 MsG0480021872.01.T01:intron 60.0%
AGCGCCGCCCCATCACAAAT+CGG - Chr4:65418759-65418778 MsG0480021872.01.T01:intron 60.0%
AGCGGGTCGTCATCTTCGTG+AGG + Chr4:65418826-65418845 None:intergenic 60.0%
CGATGGAGCGCAGAGAGGAT+GGG + Chr4:65418789-65418808 None:intergenic 60.0%
GGAGGTCGTAGTAACGACGG+AGG - Chr4:65418919-65418938 MsG0480021872.01.T01:intron 60.0%
GGCGAGAGTGACGAAGATGG+TGG + Chr4:65418733-65418752 None:intergenic 60.0%
GTAGTGCAGCCAGATGCCTC+AGG + Chr4:65419653-65419672 None:intergenic 60.0%
! TTGTGATGGGGCGGCGCTTA+CGG + Chr4:65418757-65418776 None:intergenic 60.0%
!! GCTGGATGGTGGAGGGACAA+GGG + Chr4:65418700-65418719 None:intergenic 60.0%
GCGATGGAGCGCAGAGAGGA+TGG + Chr4:65418790-65418809 None:intergenic 65.0%
GGATGCGATGGAGCGCAGAG+AGG + Chr4:65418794-65418813 None:intergenic 65.0%
GGCGGCGAGAGTGACGAAGA+TGG + Chr4:65418736-65418755 None:intergenic 65.0%
GGGTCGTGTAAGAGGGAGGG+TGG + Chr4:65418680-65418699 None:intergenic 65.0%
! GCTGAGGACCCTGAGGCATC+TGG - Chr4:65419641-65419660 MsG0480021872.01.T01:intron 65.0%
!! AGCGCCGGTCACGGTCGTAT+CGG - Chr4:65418878-65418897 MsG0480021872.01.T01:intron 65.0%
!! CGAAGATGGTGGTGGTGCGG+TGG + Chr4:65418722-65418741 None:intergenic 65.0%
!! GGCTGGATGGTGGAGGGACA+AGG + Chr4:65418701-65418720 None:intergenic 65.0%
!! TGCGGTGGCTGGATGGTGGA+GGG + Chr4:65418707-65418726 None:intergenic 65.0%
CTGTGGGGCTGAGGACCCTG+AGG - Chr4:65419634-65419653 MsG0480021872.01.T01:intron 70.0%
GTGGTGCGGTGGCTGGATGG+TGG + Chr4:65418711-65418730 None:intergenic 70.0%
TGATGGGGCGGCGCTTACGG+CGG + Chr4:65418754-65418773 None:intergenic 70.0%
! GTGCGGTGGCTGGATGGTGG+AGG + Chr4:65418708-65418727 None:intergenic 70.0%
! GTGGTGGTGCGGTGGCTGGA+TGG + Chr4:65418714-65418733 None:intergenic 70.0%
!! GATGGTGGTGGTGCGGTGGC+TGG + Chr4:65418718-65418737 None:intergenic 70.0%
!! GGTCACGTGAGCGCCGGTCA+CGG - Chr4:65418869-65418888 MsG0480021872.01.T01:intron 70.0%
Chromosome Type Strat End Strand Name
Chr4 gene 65416192 65420324 65416192 ID=MsG0480021872.01;
Chr4 mRNA 65416192 65420324 65416192 ID=MsG0480021872.01.T01;Parent=MsG0480021872.01
Chr4 exon 65416192 65416936 65416192 ID=MsG0480021872.01.T01.exon;Parent=MsG0480021872.01.T01
Chr4 CDS 65416813 65416936 65416813 ID=MsG0480021872.01.T01.cds;Parent=MsG0480021872.01.T01
Chr4 CDS 65420099 65420283 65420099 ID=MsG0480021872.01.T01.cds;Parent=MsG0480021872.01.T01
Chr4 exon 65420099 65420324 65420099 ID=MsG0480021872.01.T01.exon;Parent=MsG0480021872.01.T01
Gene Sequence

>MsG0480021872.01.T01

ATGGAGAAGAGTGAGAAAGAGGTGTCAAATTCAAATTCAATATCAAAGGTAGGAGTAGGAGTAGGAGTAGGAGGAGTATTATGCAAGAGATGCAAGCAAACGTATAATCCTGCTTCCAATTCTTCAACTTCTTGCCGTTTCCACCCTTCCTTCTTCGTATGTCGCCGTCACGACGACCAAAAAAGGTATTATGAACTGGGCCCGGATGATCCACCCTATGCTGCGAAATTCTATGATTGCTGTGGGGCTGAGGACCCTGAGGCATCTGGCTGCACTACATACTTTCATGTCTCTTATGATGAAGACTGA
Protein sequence

>MsG0480021872.01.T01

MEKSEKEVSNSNSISKVGVGVGVGGVLCKRCKQTYNPASNSSTSCRFHPSFFVCRRHDDQKRYYELGPDDPPYAAKFYDCCGAEDPEASGCTTYFHVSYDED*