Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032516.01.T01 | XP_003620539.1 | 83.451 | 284 | 14 | 2 | 1 | 251 | 1 | 284 | 1.92E-165 | 469 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032516.01.T01 | Q9SQR3 | 36.194 | 268 | 136 | 5 | 10 | 245 | 1 | 265 | 1.46E-52 | 174 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032516.01.T01 | G7KNK3 | 83.451 | 284 | 14 | 2 | 1 | 251 | 1 | 284 | 9.15e-166 | 469 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048802.01 | MsG0680032516.01 | 0.800270 | 1.559090e-48 | 7.228791e-46 |
| MsG0080048849.01 | MsG0680032516.01 | 0.834440 | 2.993684e-56 | 3.553010e-53 |
| MsG0080049069.01 | MsG0680032516.01 | 0.829254 | 5.706749e-55 | 5.809862e-52 |
| MsG0080049147.01 | MsG0680032516.01 | 0.805321 | 1.412592e-49 | 7.450903e-47 |
| MsG0180000509.01 | MsG0680032516.01 | 0.800189 | 1.619407e-48 | 7.492818e-46 |
| MsG0180002700.01 | MsG0680032516.01 | 0.842524 | 2.452999e-58 | 3.729550e-55 |
| MsG0180003357.01 | MsG0680032516.01 | 0.806458 | 8.147211e-50 | 4.424439e-47 |
| MsG0180003821.01 | MsG0680032516.01 | 0.826664 | 2.396261e-54 | 2.261804e-51 |
| MsG0180004025.01 | MsG0680032516.01 | 0.842458 | 2.553741e-58 | 3.874945e-55 |
| MsG0180004493.01 | MsG0680032516.01 | 0.807807 | 4.220635e-50 | 2.373481e-47 |
| MsG0180005220.01 | MsG0680032516.01 | 0.818133 | 2.298340e-52 | 1.705949e-49 |
| MsG0180005240.01 | MsG0680032516.01 | 0.825963 | 3.519134e-54 | 3.256079e-51 |
| MsG0180005303.01 | MsG0680032516.01 | -0.810340 | 1.210390e-50 | 7.276967e-48 |
| MsG0180006025.01 | MsG0680032516.01 | 0.809696 | 1.665825e-50 | 9.847896e-48 |
| MsG0180006096.01 | MsG0680032516.01 | 0.821603 | 3.698226e-53 | 3.022577e-50 |
| MsG0180006104.01 | MsG0680032516.01 | 0.849112 | 3.985765e-60 | 7.477482e-57 |
| MsG0280006306.01 | MsG0680032516.01 | -0.822926 | 1.824027e-53 | 1.548036e-50 |
| MsG0280010564.01 | MsG0680032516.01 | 0.821442 | 4.028932e-53 | 3.278137e-50 |
| MsG0280010916.01 | MsG0680032516.01 | 0.809340 | 1.986159e-50 | 1.163114e-47 |
| MsG0280011073.01 | MsG0680032516.01 | 0.892407 | 1.632683e-74 | 1.490737e-70 |
| MsG0280011255.01 | MsG0680032516.01 | 0.816466 | 5.450860e-52 | 3.865180e-49 |
| MsG0280011287.01 | MsG0680032516.01 | 0.829338 | 5.442225e-55 | 5.554575e-52 |
| MsG0280011376.01 | MsG0680032516.01 | 0.806112 | 9.633486e-50 | 5.185548e-47 |
| MsG0280011401.01 | MsG0680032516.01 | 0.827070 | 1.916574e-54 | 1.830462e-51 |
| MsG0380012005.01 | MsG0680032516.01 | 0.829025 | 6.482699e-55 | 6.554286e-52 |
| MsG0380012184.01 | MsG0680032516.01 | 0.832717 | 8.058678e-56 | 9.083090e-53 |
| MsG0380012567.01 | MsG0680032516.01 | 0.834094 | 3.656750e-56 | 4.294867e-53 |
| MsG0380012868.01 | MsG0680032516.01 | 0.822272 | 2.588708e-53 | 2.156556e-50 |
| MsG0380013050.01 | MsG0680032516.01 | -0.819357 | 1.212097e-52 | 9.305605e-50 |
| MsG0380013467.01 | MsG0680032516.01 | 0.835074 | 2.072865e-56 | 2.506998e-53 |
| MsG0380013471.01 | MsG0680032516.01 | 0.811777 | 5.908425e-51 | 3.690238e-48 |
| MsG0380013853.01 | MsG0680032516.01 | 0.817921 | 2.565390e-52 | 1.893238e-49 |
| MsG0380014727.01 | MsG0680032516.01 | 0.834211 | 3.417522e-56 | 4.027866e-53 |
| MsG0380014866.01 | MsG0680032516.01 | 0.816067 | 6.692899e-52 | 4.694805e-49 |
| MsG0680032516.01 | MsG0680032830.01 | 0.800408 | 1.461873e-48 | 6.801735e-46 |
| MsG0680032516.01 | MsG0680033465.01 | 0.803974 | 2.697799e-49 | 1.374565e-46 |
| MsG0680032516.01 | MsG0680034235.01 | 0.803682 | 3.103190e-49 | 1.569180e-46 |
| MsG0680032516.01 | MsG0680035618.01 | 0.945583 | 2.326975e-104 | 3.599412e-99 |
| MsG0680032516.01 | MsG0780035960.01 | 0.838925 | 2.152162e-57 | 2.925659e-54 |
| MsG0680032516.01 | MsG0780036803.01 | 0.806099 | 9.695710e-50 | 5.217270e-47 |
| MsG0680032516.01 | MsG0780036978.01 | 0.800216 | 1.599630e-48 | 7.406394e-46 |
| MsG0680032516.01 | MsG0780037330.01 | 0.834993 | 2.173524e-56 | 2.622630e-53 |
| MsG0680032516.01 | MsG0780037399.01 | 0.804368 | 2.234301e-49 | 1.149952e-46 |
| MsG0680032516.01 | MsG0780037701.01 | 0.822907 | 1.842629e-53 | 1.563000e-50 |
| MsG0680032516.01 | MsG0780039438.01 | 0.813636 | 2.315318e-51 | 1.520135e-48 |
| MsG0680032516.01 | MsG0780039806.01 | 0.802827 | 4.663548e-49 | 2.307408e-46 |
| MsG0680032516.01 | MsG0780039964.01 | 0.822323 | 2.519433e-53 | 2.101893e-50 |
| MsG0680032516.01 | MsG0780040375.01 | -0.835168 | 1.963234e-56 | 2.381366e-53 |
| MsG0680032516.01 | MsG0780040794.01 | 0.873228 | 1.730524e-67 | 7.453673e-64 |
| MsG0680032516.01 | MsG0780040825.01 | -0.809153 | 2.177790e-50 | 1.268993e-47 |
| MsG0680032516.01 | MsG0780041729.01 | -0.879562 | 1.126473e-69 | 6.134933e-66 |
| MsG0680032516.01 | MsG0780041747.01 | 0.858715 | 6.810498e-63 | 1.754426e-59 |
| MsG0680032516.01 | MsG0880042086.01 | 0.827417 | 1.582429e-54 | 1.526880e-51 |
| MsG0680032516.01 | MsG0880042794.01 | 0.824358 | 8.428170e-54 | 7.448039e-51 |
| MsG0680032516.01 | MsG0880043107.01 | 0.803529 | 3.337782e-49 | 1.681330e-46 |
| MsG0680032516.01 | MsG0880043829.01 | 0.817207 | 3.716432e-52 | 2.689406e-49 |
| MsG0680032516.01 | MsG0880044524.01 | 0.802861 | 4.588555e-49 | 2.272083e-46 |
| MsG0680032516.01 | MsG0880045190.01 | 0.800029 | 1.745472e-48 | 8.043401e-46 |
| MsG0680032516.01 | MsG0880045544.01 | 0.824739 | 6.855181e-54 | 6.125644e-51 |
| MsG0680032516.01 | MsG0880046434.01 | -0.810450 | 1.146043e-50 | 6.910195e-48 |
| MsG0680032516.01 | MsG0880046599.01 | -0.800579 | 1.349023e-48 | 6.303984e-46 |
| MsG0680032516.01 | MsG0880046877.01 | -0.853290 | 2.635178e-61 | 5.665487e-58 |
| MsG0680032516.01 | MsG0880047152.01 | 0.824526 | 7.696926e-54 | 6.834982e-51 |
| MsG0680032516.01 | MsG0880047481.01 | -0.806976 | 6.332296e-50 | 3.484858e-47 |
| MsG0680032516.01 | MsG0880047696.01 | 0.813971 | 1.953644e-51 | 1.294368e-48 |
| MsG0480018519.01 | MsG0680032516.01 | 0.812326 | 4.485141e-51 | 2.843259e-48 |
| MsG0480018850.01 | MsG0680032516.01 | 0.820230 | 7.653932e-53 | 6.021299e-50 |
| MsG0480018911.01 | MsG0680032516.01 | 0.854805 | 9.639362e-62 | 2.179312e-58 |
| MsG0480019148.01 | MsG0680032516.01 | 0.816209 | 6.223103e-52 | 4.382231e-49 |
| MsG0480019384.01 | MsG0680032516.01 | 0.813589 | 2.371564e-51 | 1.555103e-48 |
| MsG0480020024.01 | MsG0680032516.01 | 0.874259 | 7.764802e-68 | 3.472161e-64 |
| MsG0480020025.01 | MsG0680032516.01 | 0.813575 | 2.388425e-51 | 1.565551e-48 |
| MsG0480021160.01 | MsG0680032516.01 | 0.815028 | 1.139985e-51 | 7.772129e-49 |
| MsG0480021776.01 | MsG0680032516.01 | -0.823004 | 1.749087e-53 | 1.487753e-50 |
| MsG0480021872.01 | MsG0680032516.01 | -0.839572 | 1.462816e-57 | 2.028299e-54 |
| MsG0480022155.01 | MsG0680032516.01 | 0.854563 | 1.132467e-61 | 2.539659e-58 |
| MsG0480022239.01 | MsG0680032516.01 | 0.873185 | 1.788285e-67 | 7.690472e-64 |
| MsG0480022240.01 | MsG0680032516.01 | 0.807032 | 6.160858e-50 | 3.395504e-47 |
| MsG0480022243.01 | MsG0680032516.01 | 0.818515 | 1.883268e-52 | 1.412715e-49 |
| MsG0480022245.01 | MsG0680032516.01 | 0.857741 | 1.327271e-62 | 3.309403e-59 |
| MsG0480022246.01 | MsG0680032516.01 | 0.809303 | 2.022386e-50 | 1.183155e-47 |
| MsG0480022248.01 | MsG0680032516.01 | 0.826447 | 2.699266e-54 | 2.532124e-51 |
| MsG0480022401.01 | MsG0680032516.01 | 0.809049 | 2.293138e-50 | 1.332432e-47 |
| MsG0480022535.01 | MsG0680032516.01 | 0.833027 | 6.750623e-56 | 7.679638e-53 |
| MsG0480022739.01 | MsG0680032516.01 | -0.800942 | 1.137407e-48 | 5.364457e-46 |
| MsG0480022763.01 | MsG0680032516.01 | 0.808775 | 2.624013e-50 | 1.513536e-47 |
| MsG0480023073.01 | MsG0680032516.01 | 0.807513 | 4.871578e-50 | 2.718809e-47 |
| MsG0480023652.01 | MsG0680032516.01 | 0.819561 | 1.088768e-52 | 8.407249e-50 |
| MsG0480023867.01 | MsG0680032516.01 | 0.810437 | 1.153234e-50 | 6.951058e-48 |
| MsG0580024491.01 | MsG0680032516.01 | -0.814642 | 1.388803e-51 | 9.370313e-49 |
| MsG0580024597.01 | MsG0680032516.01 | 0.862085 | 6.506611e-64 | 1.881870e-60 |
| MsG0580024598.01 | MsG0680032516.01 | 0.837782 | 4.241637e-57 | 5.569125e-54 |
| MsG0580024600.01 | MsG0680032516.01 | 0.840131 | 1.045712e-57 | 1.475559e-54 |
| MsG0580024601.01 | MsG0680032516.01 | 0.837122 | 6.263051e-57 | 8.056528e-54 |
| MsG0580024790.01 | MsG0680032516.01 | -0.812504 | 4.100257e-51 | 2.611624e-48 |
| MsG0580024930.01 | MsG0680032516.01 | -0.844385 | 7.811373e-59 | 1.258896e-55 |
| MsG0380015215.01 | MsG0680032516.01 | -0.817124 | 3.880548e-52 | 2.801736e-49 |
| MsG0380015382.01 | MsG0680032516.01 | 0.831015 | 2.121001e-55 | 2.272966e-52 |
| MsG0380015533.01 | MsG0680032516.01 | 0.820192 | 7.808184e-53 | 6.136704e-50 |
| MsG0380015745.01 | MsG0680032516.01 | 0.839272 | 1.750310e-57 | 2.404751e-54 |
| MsG0380015847.01 | MsG0680032516.01 | -0.828444 | 8.959445e-55 | 8.907200e-52 |
| MsG0380016118.01 | MsG0680032516.01 | -0.812096 | 5.034867e-51 | 3.171797e-48 |
| MsG0380016191.01 | MsG0680032516.01 | -0.807001 | 6.254993e-50 | 3.444591e-47 |
| MsG0380016347.01 | MsG0680032516.01 | -0.817000 | 4.136713e-52 | 2.976879e-49 |
| MsG0380016353.01 | MsG0680032516.01 | 0.832041 | 1.185533e-55 | 1.309681e-52 |
| MsG0380016740.01 | MsG0680032516.01 | 0.868128 | 8.219748e-66 | 2.940640e-62 |
| MsG0380017023.01 | MsG0680032516.01 | 0.809464 | 1.868451e-50 | 1.097757e-47 |
| MsG0380017085.01 | MsG0680032516.01 | 0.830768 | 2.438640e-55 | 2.594395e-52 |
| MsG0380017322.01 | MsG0680032516.01 | 0.823859 | 1.104035e-53 | 9.620443e-51 |
| MsG0380017338.01 | MsG0680032516.01 | 0.817072 | 3.986290e-52 | 2.874098e-49 |
| MsG0380017437.01 | MsG0680032516.01 | -0.811434 | 7.014973e-51 | 4.341938e-48 |
| MsG0380017931.01 | MsG0680032516.01 | 0.828368 | 9.346791e-55 | 9.271204e-52 |
| MsG0480018402.01 | MsG0680032516.01 | 0.802096 | 6.598760e-49 | 3.204329e-46 |
| MsG0480018426.01 | MsG0680032516.01 | 0.804602 | 1.996496e-49 | 1.033762e-46 |
| MsG0580026488.01 | MsG0680032516.01 | -0.847403 | 1.181981e-59 | 2.098577e-56 |
| MsG0580027103.01 | MsG0680032516.01 | 0.826829 | 2.187957e-54 | 2.074972e-51 |
| MsG0580029734.01 | MsG0680032516.01 | 0.822349 | 2.483831e-53 | 2.073825e-50 |
| MsG0580029912.01 | MsG0680032516.01 | 0.814560 | 1.447460e-51 | 9.744802e-49 |
| MsG0580029914.01 | MsG0680032516.01 | 0.849059 | 4.122783e-60 | 7.720969e-57 |
| MsG0580029919.01 | MsG0680032516.01 | 0.817169 | 3.790437e-52 | 2.740131e-49 |
| MsG0680030555.01 | MsG0680032516.01 | 0.815385 | 9.499765e-52 | 6.540096e-49 |
| MsG0680030636.01 | MsG0680032516.01 | 0.824589 | 7.438328e-54 | 6.617973e-51 |
| MsG0280006634.01 | MsG0680032516.01 | 0.805454 | 1.324472e-49 | 7.010192e-47 |
| MsG0280006669.01 | MsG0680032516.01 | 0.833377 | 5.521553e-56 | 6.347556e-53 |
| MsG0280006753.01 | MsG0680032516.01 | -0.800343 | 1.506792e-48 | 6.999326e-46 |
| MsG0280007165.01 | MsG0680032516.01 | -0.895227 | 1.171066e-75 | 1.204221e-71 |
| MsG0280007513.01 | MsG0680032516.01 | -0.806659 | 7.386748e-50 | 4.031835e-47 |
| MsG0280007743.01 | MsG0680032516.01 | -0.841958 | 3.464314e-58 | 5.173803e-55 |
| MsG0280007756.01 | MsG0680032516.01 | 0.828030 | 1.127476e-54 | 1.107481e-51 |
| MsG0280008928.01 | MsG0680032516.01 | 0.859174 | 4.964261e-63 | 1.299215e-59 |
| MsG0280008929.01 | MsG0680032516.01 | 0.883775 | 3.372023e-71 | 2.167441e-67 |
| MsG0280008932.01 | MsG0680032516.01 | 0.804125 | 2.509522e-49 | 1.283565e-46 |
| MsG0280009256.01 | MsG0680032516.01 | 0.811686 | 6.184899e-51 | 3.853425e-48 |
| MsG0280009685.01 | MsG0680032516.01 | 0.825761 | 3.929877e-54 | 3.615173e-51 |
| MsG0280009756.01 | MsG0680032516.01 | 0.816038 | 6.795382e-52 | 4.762893e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880045960.01 | MsG0680032516.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032516.01.T01 | MTR_6g086560 | 83.451 | 284 | 14 | 2 | 1 | 251 | 1 | 284 | 2.32e-169 | 469 |
| MsG0680032516.01.T01 | MTR_3g045440 | 50.741 | 270 | 100 | 2 | 9 | 246 | 1 | 269 | 4.38e-92 | 272 |
| MsG0680032516.01.T01 | MTR_1g018320 | 36.328 | 256 | 128 | 4 | 20 | 242 | 10 | 263 | 1.35e-53 | 174 |
| MsG0680032516.01.T01 | MTR_4g095310 | 32.389 | 247 | 132 | 5 | 21 | 234 | 9 | 253 | 2.71e-42 | 145 |
| MsG0680032516.01.T01 | MTR_5g016150 | 32.283 | 254 | 137 | 4 | 21 | 241 | 9 | 260 | 1.20e-41 | 144 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680032516.01.T01 | AT3G24420 | 44.074 | 270 | 117 | 3 | 11 | 247 | 5 | 273 | 2.24e-79 | 240 |
| MsG0680032516.01.T01 | AT3G03990 | 36.194 | 268 | 136 | 5 | 10 | 245 | 1 | 265 | 1.49e-53 | 174 |
| MsG0680032516.01.T01 | AT4G37470 | 31.890 | 254 | 138 | 4 | 21 | 241 | 9 | 260 | 1.25e-41 | 144 |
Find 39 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGAATCCAACTATGAAAAT+TGG | 0.176582 | 6:+43559034 | MsG0680032516.01.T01:CDS |
| TCAAAGGGTCATAAAGATTT+TGG | 0.191944 | 6:-43556304 | None:intergenic |
| AGATGACTTGATCAGTTTAT+TGG | 0.223698 | 6:+43556352 | MsG0680032516.01.T01:CDS |
| TACATGGAGAAGAAAATTAA+AGG | 0.257111 | 6:+43559273 | MsG0680032516.01.T01:CDS |
| TTCACTGACGGTTCATCATT+TGG | 0.283045 | 6:-43559087 | None:intergenic |
| TTGGAAGTAATTGACACTTT+TGG | 0.304639 | 6:+43559306 | MsG0680032516.01.T01:CDS |
| GTCATTGCAAAACACTGTTT+TGG | 0.306816 | 6:-43559163 | None:intergenic |
| TCTTTAACAGCACCAGAAAA+AGG | 0.312331 | 6:-43556281 | None:intergenic |
| TCAGCTAGTTGATGTGCTTA+AGG | 0.333933 | 6:+43559356 | MsG0680032516.01.T01:CDS |
| ATGGAACAGACCAATCAATA+TGG | 0.335818 | 6:+43556201 | MsG0680032516.01.T01:CDS |
| ATAGAGTTGTTCTATTTGAT+TGG | 0.343427 | 6:+43556258 | MsG0680032516.01.T01:CDS |
| AACATGTTTGGTTTGGTGTT+TGG | 0.345702 | 6:-43556095 | None:intergenic |
| ATGTAGAGAGCTACACTATA+TGG | 0.346840 | 6:-43559255 | None:intergenic |
| CAGCTAGTTGATGTGCTTAA+GGG | 0.371050 | 6:+43559357 | MsG0680032516.01.T01:CDS |
| TTATGCTGTTCTAACATGTT+TGG | 0.379798 | 6:-43556107 | None:intergenic |
| GATCAGTTTATTGGATCAAA+TGG | 0.398901 | 6:+43556361 | MsG0680032516.01.T01:CDS |
| AGAATCCAACTATGAAAATT+GGG | 0.411548 | 6:+43559035 | MsG0680032516.01.T01:CDS |
| ATTTGGATCCACTACATTTG+TGG | 0.429468 | 6:-43559070 | None:intergenic |
| CTGTTCTAACATGTTTGGTT+TGG | 0.431637 | 6:-43556102 | None:intergenic |
| GCTTAAAGTTGTTAACTTTG+TGG | 0.445334 | 6:+43556385 | MsG0680032516.01.T01:CDS |
| CTTCTGAATGCTAGAATCCT+AGG | 0.449257 | 6:+43556143 | MsG0680032516.01.T01:CDS |
| AATGCTAGAATCCTAGGAAC+AGG | 0.457236 | 6:+43556149 | MsG0680032516.01.T01:CDS |
| TAGTTTCTGTTCCTGTTCCT+AGG | 0.461589 | 6:-43556160 | None:intergenic |
| TATAAATACAGATGACTATG+AGG | 0.467980 | 6:+43558969 | MsG0680032516.01.T01:intron |
| CTAAAGAAGAATACTTCAAA+GGG | 0.474943 | 6:-43556319 | None:intergenic |
| ACATGGAGAAGAAAATTAAA+GGG | 0.480634 | 6:+43559274 | MsG0680032516.01.T01:CDS |
| AATTAAAGGGAAAGTTACAT+TGG | 0.494880 | 6:+43559287 | MsG0680032516.01.T01:CDS |
| TTATTTCTCCACAAATGTAG+TGG | 0.511610 | 6:+43559062 | MsG0680032516.01.T01:CDS |
| TCTAAAGAAGAATACTTCAA+AGG | 0.526059 | 6:-43556320 | None:intergenic |
| AGCACATCAACTAGCTGAAG+TGG | 0.527501 | 6:-43559351 | None:intergenic |
| TGGAACAGACCAATCAATAT+GGG | 0.528463 | 6:+43556202 | MsG0680032516.01.T01:CDS |
| GAGGTTTGAATAATTGTGCA+TGG | 0.540705 | 6:-43559216 | None:intergenic |
| TCTCTGAATTTGTTCACTGA+CGG | 0.550900 | 6:-43559099 | None:intergenic |
| ATATAGTGTAGCTCTCTACA+TGG | 0.592180 | 6:+43559257 | MsG0680032516.01.T01:CDS |
| TGAAGTGGAGCAGTTAGTTG+AGG | 0.612568 | 6:-43559336 | None:intergenic |
| ATAAATACAGATGACTATGA+GGG | 0.625313 | 6:+43558970 | MsG0680032516.01.T01:intron |
| TATTCAAACCTCTAGTGACA+TGG | 0.628354 | 6:+43559227 | MsG0680032516.01.T01:CDS |
| TGGAACAACCATGTCACTAG+AGG | 0.653480 | 6:-43559235 | None:intergenic |
| AATACAGATGACTATGAGGG+AGG | 0.668351 | 6:+43558973 | MsG0680032516.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAGTAATTAATATTCTTAT+AGG | - | Chr6:43557191-43557210 | None:intergenic | 10.0% |
| !! | TAAAGAATAAAATAACTATA+TGG | + | Chr6:43557800-43557819 | MsG0680032516.01.T01:intron | 10.0% |
| !! | TAGTAATTAATATTCTTATA+GGG | - | Chr6:43557190-43557209 | None:intergenic | 10.0% |
| !!! | AAAAGTCATAAATTTTTTTT+TGG | + | Chr6:43557354-43557373 | MsG0680032516.01.T01:intron | 10.0% |
| !!! | CATAAAATAGTTTAAATTTT+AGG | + | Chr6:43557120-43557139 | MsG0680032516.01.T01:intron | 10.0% |
| !!! | TATCTTTTTTTTTTTTTTGA+GGG | - | Chr6:43558908-43558927 | None:intergenic | 10.0% |
| !!! | TTATCTTTTTTTTTTTTTTG+AGG | - | Chr6:43558909-43558928 | None:intergenic | 10.0% |
| !! | AATAGATAATTATAAACATG+TGG | - | Chr6:43558315-43558334 | None:intergenic | 15.0% |
| !! | AATTTAGATCTTCAAATTAT+AGG | + | Chr6:43557392-43557411 | MsG0680032516.01.T01:intron | 15.0% |
| !!! | AAAATCTTTCTTTTATATGT+GGG | + | Chr6:43557899-43557918 | MsG0680032516.01.T01:intron | 15.0% |
| !!! | AATTTAGAATTGAAATGAAA+TGG | + | Chr6:43557059-43557078 | MsG0680032516.01.T01:intron | 15.0% |
| !!! | ACTCTATATTTATTTTTTGT+CGG | - | Chr6:43558101-43558120 | None:intergenic | 15.0% |
| !!! | TAGTTTTTTTCAGTAAAATA+AGG | - | Chr6:43556239-43556258 | None:intergenic | 15.0% |
| !!! | TATTTTTATTTGTTAGTTAG+AGG | - | Chr6:43558134-43558153 | None:intergenic | 15.0% |
| !!! | TTATATACTTTTTTTTGTGT+CGG | - | Chr6:43557955-43557974 | None:intergenic | 15.0% |
| !! | AAAATGACAAATGTTAAGAA+AGG | - | Chr6:43556981-43557000 | None:intergenic | 20.0% |
| !! | AAACTAGAGATTTAGTAAAA+AGG | - | Chr6:43557322-43557341 | None:intergenic | 20.0% |
| !! | AAATGACAAATGTTAAGAAA+GGG | - | Chr6:43556980-43556999 | None:intergenic | 20.0% |
| !! | AATAATTATAGTGATGTAAG+TGG | - | Chr6:43558179-43558198 | None:intergenic | 20.0% |
| !! | AATTAATATTCTTATAGGGA+TGG | - | Chr6:43557186-43557205 | None:intergenic | 20.0% |
| !! | ACAATAACTTTACATTTCAA+AGG | - | Chr6:43556838-43556857 | None:intergenic | 20.0% |
| !! | AGAATAAAATAACTATATGG+TGG | + | Chr6:43557803-43557822 | MsG0680032516.01.T01:intron | 20.0% |
| !! | AGTATATAACAATATACCTT+GGG | - | Chr6:43556495-43556514 | None:intergenic | 20.0% |
| !! | ATAATTATAGTGATGTAAGT+GGG | - | Chr6:43558178-43558197 | None:intergenic | 20.0% |
| !! | TAATTACTATATAGATGTGA+TGG | + | Chr6:43557201-43557220 | MsG0680032516.01.T01:intron | 20.0% |
| !! | TAGTAAAAAGGAAAATACTT+TGG | - | Chr6:43557310-43557329 | None:intergenic | 20.0% |
| !! | TATTAAAAGATAAACGTCTT+AGG | - | Chr6:43558842-43558861 | None:intergenic | 20.0% |
| !!! | AACTATTTTATGATACTACA+AGG | - | Chr6:43557112-43557131 | None:intergenic | 20.0% |
| !!! | AACTTTTAATAAAGTGTGAT+TGG | + | Chr6:43558207-43558226 | MsG0680032516.01.T01:intron | 20.0% |
| !!! | ATGTAAAGTTATTGTTTTCT+AGG | + | Chr6:43556843-43556862 | MsG0680032516.01.T01:intron | 20.0% |
| !!! | CAAAATCTTTCTTTTATATG+TGG | + | Chr6:43557898-43557917 | MsG0680032516.01.T01:intron | 20.0% |
| !!! | CACTGAAATATTTTTTTGAA+AGG | + | Chr6:43557490-43557509 | MsG0680032516.01.T01:intron | 20.0% |
| !!! | GTCATAAATTTTTTTTTGGA+AGG | + | Chr6:43557358-43557377 | MsG0680032516.01.T01:intron | 20.0% |
| !!! | TAGTTTAAATTTTAGGGAAT+TGG | + | Chr6:43557127-43557146 | MsG0680032516.01.T01:intron | 20.0% |
| ! | AAAAAGGAAAATACTTTGGT+TGG | - | Chr6:43557306-43557325 | None:intergenic | 25.0% |
| ! | AAAAGGAAAATACTTTGGTT+GGG | - | Chr6:43557305-43557324 | None:intergenic | 25.0% |
| ! | AAATCTTAAGAACAGATACT+TGG | - | Chr6:43557606-43557625 | None:intergenic | 25.0% |
| ! | AAGTATGTATACTCATTTGT+AGG | + | Chr6:43558544-43558563 | MsG0680032516.01.T01:intron | 25.0% |
| ! | AATCTCTTTGGATTTATACA+AGG | - | Chr6:43558288-43558307 | None:intergenic | 25.0% |
| ! | AATTAAAGGGAAAGTTACAT+TGG | + | Chr6:43559287-43559306 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | ACATGGAGAAGAAAATTAAA+GGG | + | Chr6:43559274-43559293 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | AGAATCCAACTATGAAAATT+GGG | + | Chr6:43559035-43559054 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | ATAAATACAGATGACTATGA+GGG | + | Chr6:43558970-43558989 | MsG0680032516.01.T01:intron | 25.0% |
| ! | ATAGAGTTGTTCTATTTGAT+TGG | + | Chr6:43556258-43556277 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | ATAGATAATGCTAAGATGTT+AGG | + | Chr6:43557842-43557861 | MsG0680032516.01.T01:intron | 25.0% |
| ! | ATTTATACAAGGAAATGTCT+TGG | - | Chr6:43558277-43558296 | None:intergenic | 25.0% |
| ! | CACAATATAAGATGATTCAA+TGG | - | Chr6:43558665-43558684 | None:intergenic | 25.0% |
| ! | CTAAAGAAGAATACTTCAAA+GGG | - | Chr6:43556322-43556341 | None:intergenic | 25.0% |
| ! | GAGTATATAACAATATACCT+TGG | - | Chr6:43556496-43556515 | None:intergenic | 25.0% |
| ! | GTAATAGACTATATGAGATA+AGG | + | Chr6:43557264-43557283 | MsG0680032516.01.T01:intron | 25.0% |
| ! | GTATATAACAATATACCTTG+GGG | - | Chr6:43556494-43556513 | None:intergenic | 25.0% |
| ! | GTTAAAACAAAATGATGTGA+TGG | + | Chr6:43558782-43558801 | MsG0680032516.01.T01:intron | 25.0% |
| ! | TAATTATAGTGATGTAAGTG+GGG | - | Chr6:43558177-43558196 | None:intergenic | 25.0% |
| ! | TACATGGAGAAGAAAATTAA+AGG | + | Chr6:43559273-43559292 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | TAGAATCCAACTATGAAAAT+TGG | + | Chr6:43559034-43559053 | MsG0680032516.01.T01:CDS | 25.0% |
| ! | TATAAATACAGATGACTATG+AGG | + | Chr6:43558969-43558988 | MsG0680032516.01.T01:intron | 25.0% |
| ! | TCTAAAGAAGAATACTTCAA+AGG | - | Chr6:43556323-43556342 | None:intergenic | 25.0% |
| ! | TGGTTCATTCAAATTTCAAA+AGG | - | Chr6:43558242-43558261 | None:intergenic | 25.0% |
| ! | TTCATTCAAATTTCAAAAGG+AGG | - | Chr6:43558239-43558258 | None:intergenic | 25.0% |
| !! | AAAATAGAGATGTCAACTTT+TGG | + | Chr6:43558414-43558433 | MsG0680032516.01.T01:intron | 25.0% |
| !! | ACCTAAAAGTTTCTTTTTGT+TGG | - | Chr6:43558347-43558366 | None:intergenic | 25.0% |
| !! | ATCTGTTCTTAAGATTTTAG+TGG | + | Chr6:43557609-43557628 | MsG0680032516.01.T01:intron | 25.0% |
| !! | CCTAAAAGTTTCTTTTTGTT+GGG | - | Chr6:43558346-43558365 | None:intergenic | 25.0% |
| !! | TATGTTGTGATTCCTTTTTA+AGG | + | Chr6:43557741-43557760 | MsG0680032516.01.T01:intron | 25.0% |
| !!! | ATTGGTTTTAATAACACCAA+AGG | - | Chr6:43558452-43558471 | None:intergenic | 25.0% |
| !!! | CTTTGTTTGTTATTGTTACT+TGG | + | Chr6:43556739-43556758 | MsG0680032516.01.T01:intron | 25.0% |
| !!! | TTTTTAAGGTGAATTTGATG+CGG | + | Chr6:43557755-43557774 | MsG0680032516.01.T01:intron | 25.0% |
| AAAGGAAAATACTTTGGTTG+GGG | - | Chr6:43557304-43557323 | None:intergenic | 30.0% | |
| AATGAACCAACCAAAACATA+TGG | + | Chr6:43557666-43557685 | MsG0680032516.01.T01:intron | 30.0% | |
| AGATGACTTGATCAGTTTAT+TGG | + | Chr6:43556352-43556371 | MsG0680032516.01.T01:CDS | 30.0% | |
| CAAACAAAGAACATAGTTAC+AGG | - | Chr6:43556728-43556747 | None:intergenic | 30.0% | |
| CTCATATAGTCTATTACAAG+TGG | - | Chr6:43557261-43557280 | None:intergenic | 30.0% | |
| GAGAACAACAAAGGAAAAAA+AGG | - | Chr6:43556939-43556958 | None:intergenic | 30.0% | |
| GATCAGTTTATTGGATCAAA+TGG | + | Chr6:43556361-43556380 | MsG0680032516.01.T01:CDS | 30.0% | |
| GCATCAAATTCACCTTAAAA+AGG | - | Chr6:43557756-43557775 | None:intergenic | 30.0% | |
| GCTTAAAGTTGTTAACTTTG+TGG | + | Chr6:43556385-43556404 | MsG0680032516.01.T01:CDS | 30.0% | |
| TGTGTCTATCTTAGAGAAAT+TGG | - | Chr6:43558470-43558489 | None:intergenic | 30.0% | |
| TTATGCTGTTCTAACATGTT+TGG | - | Chr6:43556110-43556129 | None:intergenic | 30.0% | |
| ! | AGAAACCCAATTTTCATAGT+TGG | - | Chr6:43559043-43559062 | None:intergenic | 30.0% |
| ! | ATAGTTTTTGCTCATGGTTA+TGG | + | Chr6:43556182-43556201 | MsG0680032516.01.T01:CDS | 30.0% |
| ! | CCCAACAAAAAGAAACTTTT+AGG | + | Chr6:43558343-43558362 | MsG0680032516.01.T01:intron | 30.0% |
| ! | GAAAAGTTGAGGTCTTTTAA+TGG | - | Chr6:43556446-43556465 | None:intergenic | 30.0% |
| ! | GAAACTATAGTTTTTGCTCA+TGG | + | Chr6:43556176-43556195 | MsG0680032516.01.T01:CDS | 30.0% |
| ! | TCAAAGGGTCATAAAGATTT+TGG | - | Chr6:43556307-43556326 | None:intergenic | 30.0% |
| ! | TGATTTTGTCCCATATTGAT+TGG | - | Chr6:43556214-43556233 | None:intergenic | 30.0% |
| ! | TTATTTCTCCACAAATGTAG+TGG | + | Chr6:43559062-43559081 | MsG0680032516.01.T01:CDS | 30.0% |
| ! | TTGGAAGTAATTGACACTTT+TGG | + | Chr6:43559306-43559325 | MsG0680032516.01.T01:CDS | 30.0% |
| !! | TTGGTGTCATTAAAAACCTT+TGG | + | Chr6:43558433-43558452 | MsG0680032516.01.T01:intron | 30.0% |
| ACATGTGGAAGAAATCTCTT+TGG | - | Chr6:43558300-43558319 | None:intergenic | 35.0% | |
| AGGGAATATATGCATGTTAG+AGG | - | Chr6:43556961-43556980 | None:intergenic | 35.0% | |
| ATAAGCCTCTTGAAAAGTTG+AGG | - | Chr6:43556457-43556476 | None:intergenic | 35.0% | |
| ATATAGTGTAGCTCTCTACA+TGG | + | Chr6:43559257-43559276 | MsG0680032516.01.T01:CDS | 35.0% | |
| ATGGAACAGACCAATCAATA+TGG | + | Chr6:43556201-43556220 | MsG0680032516.01.T01:CDS | 35.0% | |
| ATGTAGAGAGCTACACTATA+TGG | - | Chr6:43559258-43559277 | None:intergenic | 35.0% | |
| ATGTTAGAGGAGAACAACAA+AGG | - | Chr6:43556948-43556967 | None:intergenic | 35.0% | |
| ATTTGGATCCACTACATTTG+TGG | - | Chr6:43559073-43559092 | None:intergenic | 35.0% | |
| CATTTCAAAGGAGAAAGAAG+AGG | - | Chr6:43556826-43556845 | None:intergenic | 35.0% | |
| CTGTTCTAACATGTTTGGTT+TGG | - | Chr6:43556105-43556124 | None:intergenic | 35.0% | |
| GAGGTTTGAATAATTGTGCA+TGG | - | Chr6:43559219-43559238 | None:intergenic | 35.0% | |
| TATTCAAACCTCTAGTGACA+TGG | + | Chr6:43559227-43559246 | MsG0680032516.01.T01:CDS | 35.0% | |
| TCTTTAACAGCACCAGAAAA+AGG | - | Chr6:43556284-43556303 | None:intergenic | 35.0% | |
| TGGAACAGACCAATCAATAT+GGG | + | Chr6:43556202-43556221 | MsG0680032516.01.T01:CDS | 35.0% | |
| TGTATACTCATTTGTAGGTC+AGG | + | Chr6:43558549-43558568 | MsG0680032516.01.T01:intron | 35.0% | |
| TTCAAGAGGCTTATTCTTGT+TGG | + | Chr6:43556463-43556482 | MsG0680032516.01.T01:intron | 35.0% | |
| TTGACAATGCTAAGATCGTT+AGG | + | Chr6:43557987-43558006 | MsG0680032516.01.T01:intron | 35.0% | |
| ! | ATTTGTTAGTTAGAGGCATG+TGG | - | Chr6:43558127-43558146 | None:intergenic | 35.0% |
| ! | GTCATTGCAAAACACTGTTT+TGG | - | Chr6:43559166-43559185 | None:intergenic | 35.0% |
| ! | TCTCTGAATTTGTTCACTGA+CGG | - | Chr6:43559102-43559121 | None:intergenic | 35.0% |
| !! | AAAGACCTCAACTTTTCAAG+AGG | + | Chr6:43556449-43556468 | MsG0680032516.01.T01:intron | 35.0% |
| !! | AACATGTTTGGTTTGGTGTT+TGG | - | Chr6:43556098-43556117 | None:intergenic | 35.0% |
| !! | AGAAACTTTTAGGTACAGCT+AGG | + | Chr6:43558353-43558372 | MsG0680032516.01.T01:intron | 35.0% |
| !!! | ATGTGCTTAAGGGTGTTTTA+GGG | + | Chr6:43559367-43559386 | MsG0680032516.01.T01:CDS | 35.0% |
| !!! | CTATTTGATTGGCCTTTTTC+TGG | + | Chr6:43556269-43556288 | MsG0680032516.01.T01:CDS | 35.0% |
| AATACAGATGACTATGAGGG+AGG | + | Chr6:43558973-43558992 | MsG0680032516.01.T01:intron | 40.0% | |
| AATGCTAGAATCCTAGGAAC+AGG | + | Chr6:43556149-43556168 | MsG0680032516.01.T01:CDS | 40.0% | |
| ACAACCAATCATACCAGACA+TGG | - | Chr6:43556416-43556435 | None:intergenic | 40.0% | |
| ATACAAGGAAATGTCTTGGC+CGG | - | Chr6:43558273-43558292 | None:intergenic | 40.0% | |
| CATCTGATGTCAAGTGTGAA+CGG | - | Chr6:43558577-43558596 | None:intergenic | 40.0% | |
| CATTCCATGTCTGGTATGAT+TGG | + | Chr6:43556409-43556428 | MsG0680032516.01.T01:intron | 40.0% | |
| CTTCTGAATGCTAGAATCCT+AGG | + | Chr6:43556143-43556162 | MsG0680032516.01.T01:CDS | 40.0% | |
| TACAAGTGGCCTAGTTCATT+TGG | - | Chr6:43557247-43557266 | None:intergenic | 40.0% | |
| TAGTTTCTGTTCCTGTTCCT+AGG | - | Chr6:43556163-43556182 | None:intergenic | 40.0% | |
| TGAATGAACCACCAATTAGC+CGG | + | Chr6:43558251-43558270 | MsG0680032516.01.T01:intron | 40.0% | |
| TTCACTGACGGTTCATCATT+TGG | - | Chr6:43559090-43559109 | None:intergenic | 40.0% | |
| TTCTTCCCAAAATACTGCCT+AGG | + | Chr6:43557156-43557175 | MsG0680032516.01.T01:intron | 40.0% | |
| ! | ACATATGGTGTCAGTAGGAT+TGG | + | Chr6:43557681-43557700 | MsG0680032516.01.T01:intron | 40.0% |
| ! | CAGCTAGTTGATGTGCTTAA+GGG | + | Chr6:43559357-43559376 | MsG0680032516.01.T01:CDS | 40.0% |
| ! | CATATGGTGTCAGTAGGATT+GGG | + | Chr6:43557682-43557701 | MsG0680032516.01.T01:intron | 40.0% |
| ! | CCAAAACATATGGTGTCAGT+AGG | + | Chr6:43557676-43557695 | MsG0680032516.01.T01:intron | 40.0% |
| ! | CCTACTGACACCATATGTTT+TGG | - | Chr6:43557679-43557698 | None:intergenic | 40.0% |
| ! | GAAGTCCTAGGCAGTATTTT+GGG | - | Chr6:43557164-43557183 | None:intergenic | 40.0% |
| ! | TCAGCTAGTTGATGTGCTTA+AGG | + | Chr6:43559356-43559375 | MsG0680032516.01.T01:CDS | 40.0% |
| !!! | ACTGTTTTGGCTAAGGACAA+TGG | - | Chr6:43559153-43559172 | None:intergenic | 40.0% |
| !!! | CTGACACCATATGTTTTGGT+TGG | - | Chr6:43557675-43557694 | None:intergenic | 40.0% |
| !!! | GATGTGCTTAAGGGTGTTTT+AGG | + | Chr6:43559366-43559385 | MsG0680032516.01.T01:CDS | 40.0% |
| !!! | GCAAAACACTGTTTTGGCTA+AGG | - | Chr6:43559160-43559179 | None:intergenic | 40.0% |
| AAATGTCTTGGCCGGCTAAT+TGG | - | Chr6:43558265-43558284 | None:intergenic | 45.0% | |
| AGCACATCAACTAGCTGAAG+TGG | - | Chr6:43559354-43559373 | None:intergenic | 45.0% | |
| CACCTCATGCCAAATGAACT+AGG | + | Chr6:43557235-43557254 | MsG0680032516.01.T01:intron | 45.0% | |
| CTTATAGGGATGGAAGTCCT+AGG | - | Chr6:43557176-43557195 | None:intergenic | 45.0% | |
| CTTTGTGGTCATTCCATGTC+TGG | + | Chr6:43556400-43556419 | MsG0680032516.01.T01:intron | 45.0% | |
| TAAGGTGAATTTGATGCGGC+TGG | + | Chr6:43557759-43557778 | MsG0680032516.01.T01:intron | 45.0% | |
| TGAAGTGGAGCAGTTAGTTG+AGG | - | Chr6:43559339-43559358 | None:intergenic | 45.0% | |
| TGGAACAACCATGTCACTAG+AGG | - | Chr6:43559238-43559257 | None:intergenic | 45.0% | |
| ! | GGAAGTCCTAGGCAGTATTT+TGG | - | Chr6:43557165-43557184 | None:intergenic | 45.0% |
| !!! | ATAAAATAGTTTAAATTTTA+GGG | + | Chr6:43557121-43557140 | MsG0680032516.01.T01:intron | 5.0% |
| !!! | TATTTTATTCTTTAATTTTA+TGG | - | Chr6:43557794-43557813 | None:intergenic | 5.0% |
| !!! | TTTAAATTTTATTTAATTTC+AGG | - | Chr6:43557436-43557455 | None:intergenic | 5.0% |
| GGCCTAGTTCATTTGGCATG+AGG | - | Chr6:43557240-43557259 | None:intergenic | 50.0% | |
| GTCAGTAGGATTGGGTGAGA+GGG | + | Chr6:43557690-43557709 | MsG0680032516.01.T01:intron | 50.0% | |
| TCAGTAGGATTGGGTGAGAG+GGG | + | Chr6:43557691-43557710 | MsG0680032516.01.T01:intron | 50.0% | |
| TGTCAGTAGGATTGGGTGAG+AGG | + | Chr6:43557689-43557708 | MsG0680032516.01.T01:intron | 50.0% | |
| ! | TTCTTGTTGGTGCTTCCCCA+AGG | + | Chr6:43556476-43556495 | MsG0680032516.01.T01:intron | 50.0% |
| TGTCTTGGCCGGCTAATTGG+TGG | - | Chr6:43558262-43558281 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 43556089 | 43559407 | 43556089 | ID=MsG0680032516.01;Name=MsG0680032516.01 |
| Chr6 | mRNA | 43556089 | 43559407 | 43556089 | ID=MsG0680032516.01.T01;Parent=MsG0680032516.01;Name=MsG0680032516.01.T01;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|2|0|251 |
| Chr6 | exon | 43556089 | 43556416 | 43556089 | ID=MsG0680032516.01.T01:exon:11698;Parent=MsG0680032516.01.T01 |
| Chr6 | exon | 43558980 | 43559407 | 43558980 | ID=MsG0680032516.01.T01:exon:11697;Parent=MsG0680032516.01.T01 |
| Chr6 | CDS | 43556089 | 43556416 | 43556089 | ID=MsG0680032516.01.T01:cds;Parent=MsG0680032516.01.T01 |
| Chr6 | CDS | 43558980 | 43559407 | 43558980 | ID=MsG0680032516.01.T01:cds;Parent=MsG0680032516.01.T01 |
| Gene Sequence |
| Protein sequence |
