Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021922.01.T01 | XP_013456685.1 | 98.148 | 54 | 1 | 0 | 1 | 54 | 131 | 184 | 1.02E-29 | 112 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021922.01.T01 | Q9SS03 | 54.545 | 55 | 23 | 2 | 1 | 54 | 136 | 189 | 5.69E-13 | 62.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021922.01.T01 | A0A072UMV6 | 98.148 | 54 | 1 | 0 | 1 | 54 | 131 | 184 | 4.89e-30 | 112 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000404.01 | MsG0480021922.01 | 0.808910 | 2.455524e-50 | 1.421440e-47 |
| MsG0180003741.01 | MsG0480021922.01 | 0.815593 | 8.537996e-52 | 5.911787e-49 |
| MsG0480021922.01 | MsG0680030784.01 | 0.804215 | 2.404278e-49 | 1.232519e-46 |
| MsG0480021922.01 | MsG0680031959.01 | 0.810566 | 1.081625e-50 | 6.542071e-48 |
| MsG0480021922.01 | MsG0780036757.01 | 0.804848 | 1.773692e-49 | 9.243120e-47 |
| MsG0480021922.01 | MsG0780037341.01 | 0.812977 | 3.231528e-51 | 2.083986e-48 |
| MsG0480021922.01 | MsG0780041259.01 | 0.816839 | 4.496808e-52 | 3.221566e-49 |
| MsG0480021922.01 | MsG0780041698.01 | 0.809716 | 1.649177e-50 | 9.754824e-48 |
| MsG0480021922.01 | MsG0780041699.01 | 0.805814 | 1.113034e-49 | 5.945842e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021922.01.T01 | MTR_4g078885 | 98.148 | 54 | 1 | 0 | 1 | 54 | 131 | 184 | 1.24e-33 | 112 |
| MsG0480021922.01.T01 | MTR_3g034030 | 96.296 | 54 | 2 | 0 | 1 | 54 | 131 | 184 | 2.10e-32 | 109 |
| MsG0480021922.01.T01 | MTR_1g056370 | 58.182 | 55 | 23 | 0 | 1 | 55 | 132 | 186 | 2.71e-17 | 71.2 |
| MsG0480021922.01.T01 | MTR_7g070390 | 51.852 | 54 | 26 | 0 | 1 | 54 | 138 | 191 | 2.88e-14 | 63.2 |
| MsG0480021922.01.T01 | MTR_4g013335 | 50.000 | 54 | 27 | 0 | 1 | 54 | 142 | 195 | 2.81e-13 | 60.8 |
| MsG0480021922.01.T01 | MTR_4g013350 | 48.148 | 54 | 28 | 0 | 1 | 54 | 143 | 196 | 1.17e-12 | 59.3 |
| MsG0480021922.01.T01 | MTR_4g013325 | 48.148 | 54 | 28 | 0 | 1 | 54 | 145 | 198 | 1.25e-12 | 59.3 |
| MsG0480021922.01.T01 | MTR_4g013330 | 48.148 | 54 | 28 | 0 | 1 | 54 | 145 | 198 | 1.30e-12 | 58.9 |
| MsG0480021922.01.T01 | MTR_1g054525 | 53.704 | 54 | 23 | 2 | 1 | 54 | 140 | 191 | 1.51e-12 | 58.9 |
| MsG0480021922.01.T01 | MTR_4g013320 | 46.296 | 54 | 29 | 0 | 1 | 54 | 145 | 198 | 8.97e-12 | 57.0 |
| MsG0480021922.01.T01 | MTR_4g013345 | 46.296 | 54 | 29 | 0 | 1 | 54 | 142 | 195 | 9.61e-12 | 56.6 |
| MsG0480021922.01.T01 | MTR_4g073950 | 44.444 | 54 | 30 | 0 | 1 | 54 | 138 | 191 | 1.35e-11 | 56.2 |
| MsG0480021922.01.T01 | MTR_4g013385 | 44.444 | 54 | 30 | 0 | 1 | 54 | 136 | 189 | 6.90e-11 | 54.3 |
| MsG0480021922.01.T01 | MTR_0433s0040 | 44.444 | 54 | 30 | 0 | 1 | 54 | 136 | 189 | 6.90e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0480021922.01.T01 | AT1G65870 | 54.545 | 55 | 23 | 2 | 1 | 54 | 136 | 189 | 5.80e-14 | 62.4 |
| MsG0480021922.01.T01 | AT3G58090 | 48.148 | 54 | 28 | 0 | 1 | 54 | 218 | 271 | 2.10e-12 | 59.3 |
| MsG0480021922.01.T01 | AT3G13650 | 46.296 | 54 | 29 | 0 | 1 | 54 | 132 | 185 | 5.34e-12 | 57.4 |
| MsG0480021922.01.T01 | AT1G58170 | 48.148 | 54 | 28 | 0 | 1 | 54 | 131 | 184 | 1.35e-11 | 56.2 |
| MsG0480021922.01.T01 | AT2G21100 | 46.296 | 54 | 29 | 0 | 1 | 54 | 134 | 187 | 1.41e-11 | 56.2 |
| MsG0480021922.01.T01 | AT2G21100 | 46.296 | 54 | 29 | 0 | 1 | 54 | 134 | 187 | 1.41e-11 | 56.2 |
| MsG0480021922.01.T01 | AT1G22900 | 44.444 | 54 | 28 | 1 | 1 | 54 | 142 | 193 | 2.17e-11 | 55.8 |
| MsG0480021922.01.T01 | AT5G42500 | 48.148 | 54 | 26 | 1 | 1 | 54 | 134 | 185 | 5.33e-11 | 54.7 |
| MsG0480021922.01.T01 | AT3G13660 | 48.148 | 54 | 28 | 0 | 1 | 54 | 71 | 124 | 8.70e-11 | 53.1 |
Find 16 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAGCACGCTTAGCATGTT+AGG | 0.285369 | 4:+66023789 | None:intergenic |
| TTGGTGGGACTGGAATATTC+AGG | 0.343391 | 4:+66023853 | MsG0480021922.01.T01:CDS |
| ATTCCAGTCCCACCAACTAT+TGG | 0.424318 | 4:-66023846 | None:intergenic |
| TACTCCATAACAAAATTCTC+AGG | 0.432205 | 4:-66023924 | None:intergenic |
| ATGCCAATAGTTGGTGGGAC+TGG | 0.457329 | 4:+66023843 | MsG0480021922.01.T01:CDS |
| AATAGAGAAATGCCAATAGT+TGG | 0.500781 | 4:+66023834 | MsG0480021922.01.T01:CDS |
| TGGAATATTCAGGTTTGCTA+GGG | 0.512294 | 4:+66023863 | MsG0480021922.01.T01:CDS |
| AGAGAAATGCCAATAGTTGG+TGG | 0.536074 | 4:+66023837 | MsG0480021922.01.T01:CDS |
| GAAGCACGCTTAGCATGTTA+GGG | 0.537025 | 4:+66023790 | None:intergenic |
| CTGGAATATTCAGGTTTGCT+AGG | 0.541339 | 4:+66023862 | MsG0480021922.01.T01:CDS |
| ACTCCATAACAAAATTCTCA+GGG | 0.574234 | 4:-66023923 | None:intergenic |
| GAGAAATGCCAATAGTTGGT+GGG | 0.610676 | 4:+66023838 | MsG0480021922.01.T01:CDS |
| GGAATATTCAGGTTTGCTAG+GGG | 0.627793 | 4:+66023864 | MsG0480021922.01.T01:CDS |
| GAATATTCAGGTTTGCTAGG+GGG | 0.649233 | 4:+66023865 | MsG0480021922.01.T01:CDS |
| GCACGCTTAGCATGTTAGGG+AGG | 0.650738 | 4:+66023793 | None:intergenic |
| CTCCATAACAAAATTCTCAG+GGG | 0.687282 | 4:-66023922 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATAGAGAAATGCCAATAGT+TGG | + | Chr4:66023834-66023853 | MsG0480021922.01.T01:CDS | 30.0% | |
| ACTCCATAACAAAATTCTCA+GGG | - | Chr4:66023926-66023945 | None:intergenic | 30.0% | |
| TACTCCATAACAAAATTCTC+AGG | - | Chr4:66023927-66023946 | None:intergenic | 30.0% | |
| CTCCATAACAAAATTCTCAG+GGG | - | Chr4:66023925-66023944 | None:intergenic | 35.0% | |
| ! | TCTCTATTTTGTTCAGCAAC+AGG | - | Chr4:66023822-66023841 | None:intergenic | 35.0% |
| ! | TTCCCCTGAGAATTTTGTTA+TGG | + | Chr4:66023920-66023939 | MsG0480021922.01.T01:CDS | 35.0% |
| !! | TGGAATATTCAGGTTTGCTA+GGG | + | Chr4:66023863-66023882 | MsG0480021922.01.T01:CDS | 35.0% |
| AGAGAAATGCCAATAGTTGG+TGG | + | Chr4:66023837-66023856 | MsG0480021922.01.T01:CDS | 40.0% | |
| GAGAAATGCCAATAGTTGGT+GGG | + | Chr4:66023838-66023857 | MsG0480021922.01.T01:CDS | 40.0% | |
| ! | CTGGAATATTCAGGTTTGCT+AGG | + | Chr4:66023862-66023881 | MsG0480021922.01.T01:CDS | 40.0% |
| !! | GAATATTCAGGTTTGCTAGG+GGG | + | Chr4:66023865-66023884 | MsG0480021922.01.T01:CDS | 40.0% |
| !! | GGAATATTCAGGTTTGCTAG+GGG | + | Chr4:66023864-66023883 | MsG0480021922.01.T01:CDS | 40.0% |
| ATTCCAGTCCCACCAACTAT+TGG | - | Chr4:66023849-66023868 | None:intergenic | 45.0% | |
| TTGGTGGGACTGGAATATTC+AGG | + | Chr4:66023853-66023872 | MsG0480021922.01.T01:CDS | 45.0% | |
| !! | ATGCCAATAGTTGGTGGGAC+TGG | + | Chr4:66023843-66023862 | MsG0480021922.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 66023804 | 66023971 | 66023804 | ID=MsG0480021922.01;Name=MsG0480021922.01 |
| Chr4 | mRNA | 66023804 | 66023971 | 66023804 | ID=MsG0480021922.01.T01;Parent=MsG0480021922.01;Name=MsG0480021922.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|55 |
| Chr4 | exon | 66023804 | 66023971 | 66023804 | ID=MsG0480021922.01.T01:exon:20827;Parent=MsG0480021922.01.T01 |
| Chr4 | CDS | 66023804 | 66023971 | 66023804 | ID=MsG0480021922.01.T01:cds;Parent=MsG0480021922.01.T01 |
| Gene Sequence |
| Protein sequence |
