AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780036757.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036757.01.T01 MTR_7g018370 96.104 77 3 0 1 77 1 77 4.56e-46 152
MsG0780036757.01.T01 MTR_7g018370 82.667 75 11 1 72 146 181 253 2.07e-33 119
MsG0780036757.01.T01 MTR_6g017240 60.870 69 27 0 9 77 11 79 7.45e-22 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780036757.01.T01 AT3G02500 56.250 64 27 1 14 77 4 66 8.00e-16 72.8
MsG0780036757.01.T01 AT3G02500 56.250 64 27 1 14 77 4 66 8.09e-16 72.8
MsG0780036757.01.T01 AT3G02500 58.065 62 25 1 14 75 4 64 9.78e-16 72.4
MsG0780036757.01.T01 AT5G16030 53.521 71 27 3 12 77 2 71 2.20e-11 60.1
MsG0780036757.01.T01 AT5G16030 55.072 69 25 3 14 77 4 71 3.27e-11 60.1
MsG0780036757.01.T01 AT5G16030 55.072 69 25 3 14 77 4 71 3.53e-11 60.1
MsG0780036757.01.T01 AT5G16030 55.072 69 25 3 14 77 4 71 3.59e-11 60.1

Find 36 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTAATGTACCTTTAAA+AGG 0.181978 7:+13280239 None:intergenic
GTGTGTGACTCTTCTGAATT+TGG 0.237338 7:+13280655 None:intergenic
TTGTCTTGAAAAGAAATTAT+AGG 0.275895 7:+13280596 None:intergenic
ATCTCTTCTTCTTGAACTAT+AGG 0.294908 7:+13280430 None:intergenic
TCTCTTCTTCTTGAACTATA+GGG 0.306597 7:+13280431 None:intergenic
TTGAGTTCATAACTCATTCA+TGG 0.306645 7:-13280474 MsG0780036757.01.T01:exon
AACAATTCTAGAAGGTATTC+AGG 0.354558 7:-13280347 MsG0780036757.01.T01:CDS
GGGATATGATTCAGCATTGT+AGG 0.390690 7:+13280451 None:intergenic
CTTTGTTGTGGAATTTATAG+AGG 0.427142 7:+13280508 None:intergenic
AGAGGTTTGAAAGTAGGTGT+TGG 0.443024 7:+13280526 None:intergenic
ACACTCTCTTCTTAGAACTT+TGG 0.443499 7:-13280628 MsG0780036757.01.T01:five_prime_UTR
ATTTATAGAGGTTTGAAAGT+AGG 0.445080 7:+13280520 None:intergenic
TCTAGTACTGCTTCTTCACC+TGG 0.451475 7:-13280266 MsG0780036757.01.T01:CDS
AGAGCAACAACAATTCTAGA+AGG 0.460564 7:-13280355 MsG0780036757.01.T01:CDS
TGAAGAAGCAGTACTAGAAA+TGG 0.463289 7:+13280271 None:intergenic
ATGATGGATGGAGTTGAAGT+AGG 0.491118 7:+13279143 None:intergenic
GGTTTGAAAGTAGGTGTTGG+AGG 0.503290 7:+13280529 None:intergenic
CATAAGTGTCGTGTCGGTGT+CGG 0.503881 7:+13278795 None:intergenic
AAAGTAATGTACCTTTAAAA+GGG 0.508301 7:+13280240 None:intergenic
AGAGGGAATGACAAGAGATG+TGG 0.508546 7:-13279210 MsG0780036757.01.T01:CDS
TTTAACCTCTCTATGATGGA+TGG 0.516217 7:+13279131 None:intergenic
CTATGCAGAAAAGAAGAAAG+AGG 0.536699 7:-13279228 MsG0780036757.01.T01:intron
TGTCGTGTCGGTGTCGGACA+CGG 0.537852 7:+13278801 None:intergenic
AAAGAGTAGAATCTTTGTTG+TGG 0.545864 7:+13280496 None:intergenic
TGATGGATGGAGTTGAAGTA+GGG 0.547063 7:+13279144 None:intergenic
GATGGAGTTGAAGTAGGGCT+AGG 0.557032 7:+13279149 None:intergenic
GAGGGAATGACAAGAGATGT+GGG 0.562323 7:-13279209 MsG0780036757.01.T01:CDS
TTTGCATTTGAATTTGAACT+AGG 0.572177 7:+13279089 None:intergenic
GGACTGCTTGAACTAAGATA+AGG 0.574582 7:+13279170 None:intergenic
TATGCAGAAAAGAAGAAAGA+GGG 0.610950 7:-13279227 MsG0780036757.01.T01:intron
CTGCTTGAACTAAGATAAGG+AGG 0.612198 7:+13279173 None:intergenic
GTACCTTTAAAAGGGTAACC+AGG 0.622122 7:+13280248 None:intergenic
TGTAATCATAAGTGTCGTGT+CGG 0.626562 7:+13278789 None:intergenic
CAACTCCATCCATCATAGAG+AGG 0.653040 7:-13279136 MsG0780036757.01.T01:CDS
GAAGCTTTAGAGAAGATACA+AGG 0.659098 7:-13280313 MsG0780036757.01.T01:CDS
AGGGAATGACAAGAGATGTG+GGG 0.701802 7:-13279208 MsG0780036757.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATATTACCTTTA+AGG + Chr7:13279363-13279382 None:intergenic 10.0%
!! AAAAAAAATATTACCTTTAA+GGG + Chr7:13279362-13279381 None:intergenic 10.0%
!! AATAAGCTAAAAACAATTTA+TGG + Chr7:13280053-13280072 None:intergenic 15.0%
!! AAAAGGCAAAAGAATAATAA+AGG - Chr7:13279387-13279406 MsG0780036757.01.T01:intron 20.0%
!! AAAAGTAATGTACCTTTAAA+AGG + Chr7:13279229-13279248 None:intergenic 20.0%
!! AACAGATACTAAAATGTTTA+AGG + Chr7:13280499-13280518 None:intergenic 20.0%
!! GAAATTCATGTGAATTAAAA+TGG + Chr7:13279254-13279273 None:intergenic 20.0%
!! TTGTCTTGAAAAGAAATTAT+AGG + Chr7:13278872-13278891 None:intergenic 20.0%
!! TTTAATTCACATGAATTTCA+TGG - Chr7:13279255-13279274 MsG0780036757.01.T01:intron 20.0%
!! TTTATATTGCAGCAAAATTA+TGG - Chr7:13279474-13279493 MsG0780036757.01.T01:intron 20.0%
!!! AAAGTAATGTACCTTTAAAA+GGG + Chr7:13279228-13279247 None:intergenic 20.0%
!!! AACTTTTTGGATAAATTGAA+TGG - Chr7:13279606-13279625 MsG0780036757.01.T01:intron 20.0%
!!! AATTGTTTTTAGCTTATTTC+TGG - Chr7:13280055-13280074 MsG0780036757.01.T01:intron 20.0%
!!! ATAATTTTGCTGCAATATAA+AGG + Chr7:13279475-13279494 None:intergenic 20.0%
!!! TATTTTTGGTCTAAACTTTT+TGG - Chr7:13279593-13279612 MsG0780036757.01.T01:intron 20.0%
! AAATGCAAAAACAAAATCTG+AGG - Chr7:13280390-13280409 MsG0780036757.01.T01:CDS 25.0%
! ATTTATAGAGGTTTGAAAGT+AGG + Chr7:13278948-13278967 None:intergenic 25.0%
! CAGCAAAATTATGGAAAATA+TGG - Chr7:13279483-13279502 MsG0780036757.01.T01:intron 25.0%
! TTTGCATTTGAATTTGAACT+AGG + Chr7:13280379-13280398 None:intergenic 25.0%
!!! AAAAAGGTTTTTTAGAGTGA+AGG + Chr7:13278899-13278918 None:intergenic 25.0%
!!! AGAATCATGGTTTTAAATTG+CGG - Chr7:13279431-13279450 MsG0780036757.01.T01:intron 25.0%
!!! CAGATTTTAATTTGAGAGAA+TGG - Chr7:13279062-13279081 MsG0780036757.01.T01:intron 25.0%
AAAAACAAAATCTGAGGAAG+AGG - Chr7:13280396-13280415 MsG0780036757.01.T01:CDS 30.0%
AAAGAGTAGAATCTTTGTTG+TGG + Chr7:13278972-13278991 None:intergenic 30.0%
ATCTCTTCTTCTTGAACTAT+AGG + Chr7:13279038-13279057 None:intergenic 30.0%
CACATTGTTAACTAGAATCA+TGG - Chr7:13279418-13279437 MsG0780036757.01.T01:intron 30.0%
GCAAATAGTGACTAGAAAAA+AGG + Chr7:13278915-13278934 None:intergenic 30.0%
GGTTTGCATTAAATTCAAAG+TGG - Chr7:13279693-13279712 MsG0780036757.01.T01:intron 30.0%
TATATATGCAATCCACTTTC+AGG - Chr7:13279731-13279750 MsG0780036757.01.T01:intron 30.0%
TATGATCTAAATTTCCGTTG+CGG - Chr7:13279510-13279529 MsG0780036757.01.T01:intron 30.0%
TATGCAGAAAAGAAGAAAGA+GGG - Chr7:13280238-13280257 MsG0780036757.01.T01:intron 30.0%
TCTCTTAAATTTCCAATTCC+AGG - Chr7:13279902-13279921 MsG0780036757.01.T01:intron 30.0%
TCTCTTCTTCTTGAACTATA+GGG + Chr7:13279037-13279056 None:intergenic 30.0%
TCTTCATGTGTGTAAATTGA+TGG + Chr7:13279878-13279897 None:intergenic 30.0%
TTATCAGCAAAAACTCTTAG+TGG - Chr7:13279773-13279792 MsG0780036757.01.T01:intron 30.0%
TTGAGTTCATAACTCATTCA+TGG - Chr7:13278991-13279010 MsG0780036757.01.T01:intron 30.0%
TTTGATTCCCAATTCTACTT+CGG - Chr7:13279935-13279954 MsG0780036757.01.T01:intron 30.0%
TTTGCTGATAAACATTCCAT+TGG + Chr7:13279764-13279783 None:intergenic 30.0%
! AGTTTTTAACCTCTCTATGA+TGG + Chr7:13280341-13280360 None:intergenic 30.0%
! CATGATTCTAGTTAACAATG+TGG + Chr7:13279420-13279439 None:intergenic 30.0%
! CTTTGTTGTGGAATTTATAG+AGG + Chr7:13278960-13278979 None:intergenic 30.0%
!! AACAATTCTAGAAGGTATTC+AGG - Chr7:13279118-13279137 MsG0780036757.01.T01:CDS 30.0%
AAAGCATATGAACCTGGAAT+TGG + Chr7:13279917-13279936 None:intergenic 35.0%
AACATAGCCGAAGTAGAATT+GGG + Chr7:13279945-13279964 None:intergenic 35.0%
AAGGGTAAGCTATAACAGTT+GGG - Chr7:13279652-13279671 MsG0780036757.01.T01:intron 35.0%
AATGAAGCTCAGATACTCTT+TGG - Chr7:13280628-13280647 MsG0780036757.01.T01:five_prime_UTR 35.0%
AATTCTACTTCGGCTATGTT+CGG - Chr7:13279945-13279964 MsG0780036757.01.T01:intron 35.0%
ACACTCTCTTCTTAGAACTT+TGG - Chr7:13278837-13278856 MsG0780036757.01.T01:intron 35.0%
AGAGCAACAACAATTCTAGA+AGG - Chr7:13279110-13279129 MsG0780036757.01.T01:CDS 35.0%
AGTGGTGAAAGAATATGTTG+AGG + Chr7:13279338-13279357 None:intergenic 35.0%
CTATGCAGAAAAGAAGAAAG+AGG - Chr7:13280237-13280256 MsG0780036757.01.T01:intron 35.0%
GAATTTAATGCAAACCCTTC+TGG + Chr7:13279689-13279708 None:intergenic 35.0%
GTGGTGAAAGAATATGTTGA+GGG + Chr7:13279337-13279356 None:intergenic 35.0%
TGAAGAAGCAGTACTAGAAA+TGG + Chr7:13279197-13279216 None:intergenic 35.0%
TGATACAGCATTACAAGCAA+GGG - Chr7:13279634-13279653 MsG0780036757.01.T01:intron 35.0%
TGGTGAAAGAATATGTTGAG+GGG + Chr7:13279336-13279355 None:intergenic 35.0%
TGTCTTGCAGAATAAACAAG+AGG + Chr7:13279842-13279861 None:intergenic 35.0%
TTGATACAGCATTACAAGCA+AGG - Chr7:13279633-13279652 MsG0780036757.01.T01:intron 35.0%
! AATATTACCTTTAAGGGCAG+TGG + Chr7:13279356-13279375 None:intergenic 35.0%
! GAATCAAAAGCATATGAACC+TGG + Chr7:13279923-13279942 None:intergenic 35.0%
! GTTTTTACTGTGTATGTGAG+TGG + Chr7:13280547-13280566 None:intergenic 35.0%
! TGTAATCATAAGTGTCGTGT+CGG + Chr7:13280679-13280698 None:intergenic 35.0%
! TTTAACCTCTCTATGATGGA+TGG + Chr7:13280337-13280356 None:intergenic 35.0%
!! GAAGCTTTAGAGAAGATACA+AGG - Chr7:13279152-13279171 MsG0780036757.01.T01:CDS 35.0%
!! TTTAGCTTATTTCTGGTAGC+CGG - Chr7:13280062-13280081 MsG0780036757.01.T01:intron 35.0%
AGAGGTTTGAAAGTAGGTGT+TGG + Chr7:13278942-13278961 None:intergenic 40.0%
CAAGGGTAAGCTATAACAGT+TGG - Chr7:13279651-13279670 MsG0780036757.01.T01:intron 40.0%
CTGCTTGAACTAAGATAAGG+AGG + Chr7:13280295-13280314 None:intergenic 40.0%
GAACATAGCCGAAGTAGAAT+TGG + Chr7:13279946-13279965 None:intergenic 40.0%
GCTCAGATACTCTTTGGATT+AGG - Chr7:13280634-13280653 MsG0780036757.01.T01:five_prime_UTR 40.0%
GGACTGCTTGAACTAAGATA+AGG + Chr7:13280298-13280317 None:intergenic 40.0%
GGGATATGATTCAGCATTGT+AGG + Chr7:13279017-13279036 None:intergenic 40.0%
GTGTGTGACTCTTCTGAATT+TGG + Chr7:13278813-13278832 None:intergenic 40.0%
TAAGCATGCTACTCTAGTCT+TGG + Chr7:13279556-13279575 None:intergenic 40.0%
! GTACCTTTAAAAGGGTAACC+AGG + Chr7:13279220-13279239 None:intergenic 40.0%
! TCACCTGGTTACCCTTTTAA+AGG - Chr7:13279214-13279233 MsG0780036757.01.T01:CDS 40.0%
! TGATGGATGGAGTTGAAGTA+GGG + Chr7:13280324-13280343 None:intergenic 40.0%
!! ATGATGGATGGAGTTGAAGT+AGG + Chr7:13280325-13280344 None:intergenic 40.0%
!! GTTGTTTTCGTAAGCTATCC+TGG + Chr7:13280095-13280114 None:intergenic 40.0%
!!! ATGGTTTTAAATTGCGGTCG+CGG - Chr7:13279437-13279456 MsG0780036757.01.T01:intron 40.0%
AGAGGGAATGACAAGAGATG+TGG - Chr7:13280255-13280274 MsG0780036757.01.T01:CDS 45.0%
AGGGAATGACAAGAGATGTG+GGG - Chr7:13280257-13280276 MsG0780036757.01.T01:CDS 45.0%
ATGCAATCCACTTTCAGGAG+AGG - Chr7:13279736-13279755 MsG0780036757.01.T01:intron 45.0%
ATTGGTACCTCTCCTGAAAG+TGG + Chr7:13279746-13279765 None:intergenic 45.0%
CAACTCCATCCATCATAGAG+AGG - Chr7:13280329-13280348 MsG0780036757.01.T01:CDS 45.0%
GAGGGAATGACAAGAGATGT+GGG - Chr7:13280256-13280275 MsG0780036757.01.T01:CDS 45.0%
TCTAGTACTGCTTCTTCACC+TGG - Chr7:13279199-13279218 MsG0780036757.01.T01:CDS 45.0%
TCTTTCACCACTGCCCTTAA+AGG - Chr7:13279346-13279365 MsG0780036757.01.T01:intron 45.0%
!! ACTTTCAGGAGAGGTACCAA+TGG - Chr7:13279745-13279764 MsG0780036757.01.T01:intron 45.0%
!! GGGAGTGTTTATCTCCAGAA+GGG - Chr7:13279672-13279691 MsG0780036757.01.T01:intron 45.0%
!! GGTTTGAAAGTAGGTGTTGG+AGG + Chr7:13278939-13278958 None:intergenic 45.0%
!! TGGGAGTGTTTATCTCCAGA+AGG - Chr7:13279671-13279690 MsG0780036757.01.T01:intron 45.0%
!!! AATATTTTTTTTTTAGTAAA+AGG - Chr7:13279370-13279389 MsG0780036757.01.T01:intron 5.0%
!!! TTTCATATATTTTATATTTT+TGG - Chr7:13279579-13279598 MsG0780036757.01.T01:intron 5.0%
AAGCTATCCTGGAGAGCTAC+CGG + Chr7:13280084-13280103 None:intergenic 50.0%
ATTGCAGTCTGCAACCGCAA+CGG + Chr7:13279527-13279546 None:intergenic 50.0%
GATGGAGTTGAAGTAGGGCT+AGG + Chr7:13280319-13280338 None:intergenic 50.0%
! CATAAGTGTCGTGTCGGTGT+CGG + Chr7:13280673-13280692 None:intergenic 50.0%
!! TGTCGTGTCGGTGTCGGACA+CGG + Chr7:13280667-13280686 None:intergenic 60.0%
CTGGTAGCCGGTAGCTCTCC+AGG - Chr7:13280074-13280093 MsG0780036757.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr7 gene 13278778 13280709 13278778 ID=MsG0780036757.01;Name=MsG0780036757.01
Chr7 mRNA 13278778 13280709 13278778 ID=MsG0780036757.01.T01;Parent=MsG0780036757.01;Name=MsG0780036757.01.T01;_AED=0.47;_eAED=0.51;_QI=232|0|0.66|1|1|1|3|0|150
Chr7 exon 13278778 13278831 13278778 ID=MsG0780036757.01.T01:exon:2741;Parent=MsG0780036757.01.T01
Chr7 exon 13279070 13279242 13279070 ID=MsG0780036757.01.T01:exon:2740;Parent=MsG0780036757.01.T01
Chr7 exon 13280252 13280709 13280252 ID=MsG0780036757.01.T01:exon:2739;Parent=MsG0780036757.01.T01
Chr7 five_prime_UTR 13280478 13280709 13280478 ID=MsG0780036757.01.T01:five_prime_utr;Parent=MsG0780036757.01.T01
Chr7 CDS 13280252 13280477 13280252 ID=MsG0780036757.01.T01:cds;Parent=MsG0780036757.01.T01
Chr7 CDS 13279070 13279242 13279070 ID=MsG0780036757.01.T01:cds;Parent=MsG0780036757.01.T01
Chr7 CDS 13278778 13278831 13278778 ID=MsG0780036757.01.T01:cds;Parent=MsG0780036757.01.T01
Gene Sequence

>MsG0780036757.01.T01

ATGGCCTACAATGCTGAATCATATCCCTATAGTTCAAGAAGAAGAGATGAGTCAGATTTTAATTTGAGAGAATGGTCAGTGAAAGCAAGAATCACTAGAGAGAGCAACAACAATTCTAGAAGGTATTCAGGTTCTTATATGAGAAGCTTTAGAGAAGATACAAGGTCTCAAAGAGCAAATATAACCATTTCTAGTACTGCTTCTTCACCTGGTTACCCTTTTAAAGAAAAGAAGAAAGAGGGAATGACAAGAGATGTGGGGACTCAAAGCACACCTCCTTATCTTAGTTCAAGCAGTCCTAGCCCTACTTCAACTCCATCCATCATAGAGAGGTTAAAAACTAGAGCTGTAGACTCACCTAGTTCAAATTCAAATGCAAAAACAAAATCTGAGGAAGAGGTGTGTCGCCGTGTCCGACACCGACACGACACTTATGATTACACTGATTTTTAG

Protein sequence

>MsG0780036757.01.T01

MAYNAESYPYSSRRRDESDFNLREWSVKARITRESNNNSRRYSGSYMRSFREDTRSQRANITISSTASSPGYPFKEKKKEGMTRDVGTQSTPPYLSSSSPSPTSTPSIIERLKTRAVDSPSSNSNAKTKSEEEVCRRVRHRHDTYDYTDF*