AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000404.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000404.01.T01 MTR_1g016490 95.699 186 7 1 2 187 340 524 2.22e-128 371
MsG0180000404.01.T01 MTR_1g023050 45.570 158 74 4 4 156 275 425 2.91e-34 127
MsG0180000404.01.T01 MTR_7g106320 42.938 177 75 7 1 156 256 427 1.18e-33 126
MsG0180000404.01.T01 MTR_3g112300 47.761 134 67 2 23 156 284 414 1.34e-33 125
MsG0180000404.01.T01 MTR_3g112300 43.030 165 83 4 23 179 284 445 2.44e-33 124
MsG0180000404.01.T01 MTR_4g051532 36.816 201 99 5 1 179 300 494 9.20e-33 123
MsG0180000404.01.T01 MTR_8g078480 37.912 182 102 5 2 173 482 662 4.08e-32 122
MsG0180000404.01.T01 MTR_5g018860 39.011 182 95 6 2 174 431 605 5.34e-32 121
MsG0180000404.01.T01 MTR_1g057790 40.782 179 99 4 3 175 257 434 1.24e-31 120
MsG0180000404.01.T01 MTR_8g098815 41.139 158 83 5 2 150 276 432 3.65e-30 116
MsG0180000404.01.T01 MTR_4g019450 34.826 201 97 7 1 178 261 450 7.96e-30 115
MsG0180000404.01.T01 MTR_3g112290 41.358 162 85 4 4 156 257 417 1.36e-29 113
MsG0180000404.01.T01 MTR_7g065050 35.519 183 108 6 4 181 220 397 2.00e-29 113
MsG0180000404.01.T01 MTR_2g095050 34.343 198 110 5 4 182 339 535 2.74e-28 110
MsG0180000404.01.T01 MTR_3g112300 37.415 147 77 4 1 135 250 393 9.83e-19 82.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000404.01.T01 AT4G32980 70.186 161 41 3 2 161 292 446 4.28e-74 230
MsG0180000404.01.T01 AT4G32980 70.186 161 41 3 2 161 292 446 4.28e-74 230
MsG0180000404.01.T01 AT2G35940 45.625 160 67 6 4 148 292 446 3.06e-33 125
MsG0180000404.01.T01 AT2G35940 45.625 160 67 6 4 148 292 446 3.06e-33 125
MsG0180000404.01.T01 AT2G35940 45.625 160 67 6 4 148 292 446 3.06e-33 125
MsG0180000404.01.T01 AT2G27220 40.741 162 69 6 3 156 155 297 1.23e-32 121
MsG0180000404.01.T01 AT2G27220 40.741 162 69 6 3 156 155 297 1.23e-32 121
MsG0180000404.01.T01 AT2G27220 40.741 162 69 6 3 156 173 315 1.38e-32 121
MsG0180000404.01.T01 AT2G16400 40.000 180 98 5 4 175 195 372 8.73e-32 119
MsG0180000404.01.T01 AT4G34610 39.766 171 87 5 1 156 214 383 6.51e-31 117
MsG0180000404.01.T01 AT4G34610 39.766 171 87 5 1 156 214 383 6.51e-31 117
MsG0180000404.01.T01 AT4G34610 39.766 171 87 5 1 156 214 383 6.51e-31 117
MsG0180000404.01.T01 AT4G34610 39.766 171 87 5 1 156 214 383 6.51e-31 117
MsG0180000404.01.T01 AT4G36870 58.621 87 34 2 71 156 482 567 1.30e-29 114
MsG0180000404.01.T01 AT4G36870 58.621 87 34 2 71 156 482 567 1.30e-29 114
MsG0180000404.01.T01 AT2G23760 60.000 85 32 2 71 154 408 491 1.77e-29 114
MsG0180000404.01.T01 AT2G23760 60.000 85 32 2 71 154 408 491 1.77e-29 114
MsG0180000404.01.T01 AT2G23760 60.000 85 32 2 71 154 408 491 1.77e-29 114
MsG0180000404.01.T01 AT5G41410 42.384 151 73 5 21 159 315 463 2.09e-29 114
MsG0180000404.01.T01 AT1G19700 41.718 163 84 5 4 156 259 420 2.61e-29 113
MsG0180000404.01.T01 AT1G19700 41.718 163 84 5 4 156 259 420 2.61e-29 113
MsG0180000404.01.T01 AT1G19700 41.718 163 84 5 4 156 259 420 2.61e-29 113
MsG0180000404.01.T01 AT1G19700 41.718 163 84 5 4 156 259 420 2.61e-29 113
MsG0180000404.01.T01 AT1G19700 41.718 163 84 5 4 156 259 420 2.61e-29 113
MsG0180000404.01.T01 AT2G27990 39.869 153 87 4 4 155 344 492 4.87e-29 112
MsG0180000404.01.T01 AT1G75410 41.875 160 85 5 4 156 257 415 2.18e-28 110
MsG0180000404.01.T01 AT1G75410 41.875 160 85 5 4 156 257 415 2.18e-28 110
MsG0180000404.01.T01 AT1G75430 37.725 167 88 4 3 156 108 271 2.59e-28 107
MsG0180000404.01.T01 AT5G02030 59.420 69 27 1 86 154 348 415 1.75e-24 100
MsG0180000404.01.T01 AT2G23760 39.706 136 30 3 71 154 408 543 4.23e-23 96.3
MsG0180000404.01.T01 AT4G36870 51.515 66 30 2 71 135 482 546 8.50e-15 72.0
MsG0180000404.01.T01 AT4G36870 50.000 64 30 2 71 133 482 544 1.17e-13 68.9
MsG0180000404.01.T01 AT1G75410 34.783 138 82 5 4 134 257 393 6.35e-13 66.6

Find 35 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTTTCTGGCAAGCCCCTT+TGG 0.227378 1:+5566872 None:intergenic
TCTTTCTGGCAAGCCCCTTT+GGG 0.243439 1:+5566873 None:intergenic
AAATGCTTCTCTGCATCTTT+AGG 0.274049 1:+5566569 None:intergenic
AACGGTTTCTCGACTATATA+AGG 0.297571 1:-5567052 MsG0180000404.01.T01:CDS
CTGTTTCCGTTCTACGTGAT+TGG 0.305984 1:-5566849 MsG0180000404.01.T01:CDS
GGTTTATAAATGCGCGTGTT+CGG 0.404462 1:-5566183 MsG0180000404.01.T01:CDS
AGAACGGAAACAGATCTTTC+TGG 0.415143 1:+5566859 None:intergenic
TAAATGCGCGTGTTCGGCTA+TGG 0.423300 1:-5566177 MsG0180000404.01.T01:CDS
TTCATTCATTCAAAAGCAAT+GGG 0.425095 1:-5566935 MsG0180000404.01.T01:CDS
GCATACATTTCCTCAATCAA+TGG 0.431572 1:+5566151 None:intergenic
AAATGTTTAATATGAATTGA+AGG 0.435021 1:-5566048 None:intergenic
TAGCAAACATATTCTTTCCA+TGG 0.443732 1:-5567013 MsG0180000404.01.T01:CDS
GGGCTCTTCAACAGTTGAAA+AGG 0.453231 1:-5566915 MsG0180000404.01.T01:CDS
TGCACACTTTGCTGTCAAAA+CGG 0.454758 1:-5567070 MsG0180000404.01.T01:CDS
TGAAAAGGAAAGATCAGTTA+TGG 0.458634 1:-5566900 MsG0180000404.01.T01:CDS
CTTCATTCATTCAAAAGCAA+TGG 0.463764 1:-5566936 MsG0180000404.01.T01:CDS
GCAAAGTGTGCATGTATTTG+TGG 0.466187 1:+5567081 None:intergenic
TTCATGGTCAGAAGAGGATA+AGG 0.491594 1:-5566974 MsG0180000404.01.T01:CDS
AGCAAACATATTCTTTCCAT+GGG 0.494717 1:-5567012 MsG0180000404.01.T01:CDS
ACTGTTAAAATTTGATCCCA+TGG 0.497477 1:+5566996 None:intergenic
ATGAGAGCAGTGAAAGAAGC+AGG 0.499804 1:-5566087 MsG0180000404.01.T01:CDS
TGGTTGTGTAGGTATCAAAC+TGG 0.504145 1:-5566204 MsG0180000404.01.T01:intron
AAGTGGGTTGACTAGAAGTC+AGG 0.504398 1:-5566534 MsG0180000404.01.T01:intron
AGAAAAGCTTGTCGAAATGA+AGG 0.508701 1:-5566115 MsG0180000404.01.T01:CDS
ATCAGTTATGGAGGCCCCAA+AGG 0.509828 1:-5566888 MsG0180000404.01.T01:CDS
CTTTCTGGCAAGCCCCTTTG+GGG 0.514861 1:+5566874 None:intergenic
TCAGTTATGGAGGCCCCAAA+GGG 0.515348 1:-5566887 MsG0180000404.01.T01:CDS
GCTATGGAAACCATTGATTG+AGG 0.555071 1:-5566161 MsG0180000404.01.T01:CDS
TAACAGTTCATGGTCAGAAG+AGG 0.576067 1:-5566980 MsG0180000404.01.T01:CDS
GAACATCCAATCACGTAGAA+CGG 0.600137 1:+5566843 None:intergenic
TAGATAATACTTACGGATGG+AGG 0.617974 1:+5566813 None:intergenic
TATATAAGGACTTGAGAGAG+AGG 0.620065 1:-5567038 MsG0180000404.01.T01:CDS
TTATAGATAATACTTACGGA+TGG 0.646426 1:+5566810 None:intergenic
AAAGGAAAGATCAGTTATGG+AGG 0.667219 1:-5566897 MsG0180000404.01.T01:CDS
CAGTTATGGAGGCCCCAAAG+GGG 0.731077 1:-5566886 MsG0180000404.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAACCAAAGTTTAATATAA+AGG - Chr1:5566410-5566429 MsG0180000404.01.T01:intron 15.0%
!!! TGATCTTTTATTATAGATAA+AGG + Chr1:5566552-5566571 None:intergenic 15.0%
!!! TTTAATAACTTTTACGATAA+GGG + Chr1:5566925-5566944 None:intergenic 15.0%
!! AATAATCTTGGTCATTAATT+TGG + Chr1:5566811-5566830 None:intergenic 20.0%
!! AGTGATTAGAGTTATATTTA+TGG - Chr1:5566961-5566980 MsG0180000404.01.T01:CDS 20.0%
!! ATTAGAGTTATATTTATGGT+TGG - Chr1:5566965-5566984 MsG0180000404.01.T01:CDS 20.0%
!! ATTTACTTGCAATAAAAAGT+GGG - Chr1:5566639-5566658 MsG0180000404.01.T01:intron 20.0%
!! TCGATTATAGATAATACTTA+CGG + Chr1:5566386-5566405 None:intergenic 20.0%
!! TTTCAACTGATAAATAATCT+TGG + Chr1:5566823-5566842 None:intergenic 20.0%
!!! GTTTAATAACTTTTACGATA+AGG + Chr1:5566926-5566945 None:intergenic 20.0%
!!! TTAAAACTGATTTTGGTATT+TGG - Chr1:5566767-5566786 MsG0180000404.01.T01:intron 20.0%
! ATTTATCAGTTGAAAACATG+TGG - Chr1:5566828-5566847 MsG0180000404.01.T01:CDS 25.0%
! CATTTACTTGCAATAAAAAG+TGG - Chr1:5566638-5566657 MsG0180000404.01.T01:intron 25.0%
! GAGACCTTTATATTAAACTT+TGG + Chr1:5566417-5566436 None:intergenic 25.0%
! TTATAGATAATACTTACGGA+TGG + Chr1:5566382-5566401 None:intergenic 25.0%
! TTCATTCATTCAAAAGCAAT+GGG - Chr1:5566254-5566273 MsG0180000404.01.T01:intron 25.0%
!!! GATCAAATTTTAACAGTTCA+TGG - Chr1:5566199-5566218 MsG0180000404.01.T01:intron 25.0%
!!! GTCACATACATATTTTGATT+TGG - Chr1:5566687-5566706 MsG0180000404.01.T01:intron 25.0%
ACTGTTAAAATTTGATCCCA+TGG + Chr1:5566196-5566215 None:intergenic 30.0%
AGCAAACATATTCTTTCCAT+GGG - Chr1:5566177-5566196 MsG0180000404.01.T01:CDS 30.0%
CAAGGATTAAGTAATGTCTA+CGG + Chr1:5566864-5566883 None:intergenic 30.0%
CTTCATTCATTCAAAAGCAA+TGG - Chr1:5566253-5566272 MsG0180000404.01.T01:intron 30.0%
GATAAAAAACCTTGATACTG+TGG + Chr1:5566454-5566473 None:intergenic 30.0%
TAGCAAACATATTCTTTCCA+TGG - Chr1:5566176-5566195 MsG0180000404.01.T01:CDS 30.0%
TGAAAAGGAAAGATCAGTTA+TGG - Chr1:5566289-5566308 MsG0180000404.01.T01:intron 30.0%
! ATATTTATGGTTGGTTGTGT+AGG - Chr1:5566974-5566993 MsG0180000404.01.T01:CDS 30.0%
! CCTGACTATTAATTTGGAAT+CGG + Chr1:5566737-5566756 None:intergenic 30.0%
! GATAAGGGCTACATTTTTAA+TGG + Chr1:5566910-5566929 None:intergenic 30.0%
AAAGGAAAGATCAGTTATGG+AGG - Chr1:5566292-5566311 MsG0180000404.01.T01:intron 35.0%
AAATGCTTCTCTGCATCTTT+AGG + Chr1:5566623-5566642 None:intergenic 35.0%
AACGGTTTCTCGACTATATA+AGG - Chr1:5566137-5566156 MsG0180000404.01.T01:CDS 35.0%
ACAATGATGTGATGCATTTC+AGG - Chr1:5566594-5566613 MsG0180000404.01.T01:intron 35.0%
AGAAAAGCTTGTCGAAATGA+AGG - Chr1:5567074-5567093 MsG0180000404.01.T01:CDS 35.0%
AGATCATTGATGAATGCATG+TGG - Chr1:5566566-5566585 MsG0180000404.01.T01:CDS 35.0%
ATTAATAGTCAGGTCAGGAT+CGG - Chr1:5566744-5566763 MsG0180000404.01.T01:intron 35.0%
CCGATTCCAAATTAATAGTC+AGG - Chr1:5566734-5566753 MsG0180000404.01.T01:intron 35.0%
GCATACATTTCCTCAATCAA+TGG + Chr1:5567041-5567060 None:intergenic 35.0%
TAGATAATACTTACGGATGG+AGG + Chr1:5566379-5566398 None:intergenic 35.0%
TATATAAGGACTTGAGAGAG+AGG - Chr1:5566151-5566170 MsG0180000404.01.T01:CDS 35.0%
TCCAAATTAATAGTCAGGTC+AGG - Chr1:5566739-5566758 MsG0180000404.01.T01:intron 35.0%
TCCTGACCTGACTATTAATT+TGG + Chr1:5566743-5566762 None:intergenic 35.0%
TTAAGTAATGTCTACGGATC+CGG + Chr1:5566858-5566877 None:intergenic 35.0%
! GGTCATTAATTTGGATCGAA+TGG + Chr1:5566802-5566821 None:intergenic 35.0%
! GTTGAAAACATGTGGTTTTC+CGG - Chr1:5566836-5566855 MsG0180000404.01.T01:CDS 35.0%
!! GGATCGGTTAAAACTGATTT+TGG - Chr1:5566760-5566779 MsG0180000404.01.T01:intron 35.0%
!!! AGGTTTTTTATCCCCAATTC+CGG - Chr1:5566462-5566481 MsG0180000404.01.T01:intron 35.0%
!!! TAATCCTTGCTTGTTTTGAG+AGG - Chr1:5566875-5566894 MsG0180000404.01.T01:CDS 35.0%
AGAACGGAAACAGATCTTTC+TGG + Chr1:5566333-5566352 None:intergenic 40.0%
AGTTCCTCTCAAAACAAGCA+AGG + Chr1:5566882-5566901 None:intergenic 40.0%
GAACATCCAATCACGTAGAA+CGG + Chr1:5566349-5566368 None:intergenic 40.0%
GCAAAGTGTGCATGTATTTG+TGG + Chr1:5566111-5566130 None:intergenic 40.0%
GGTTTATAAATGCGCGTGTT+CGG - Chr1:5567006-5567025 MsG0180000404.01.T01:CDS 40.0%
TAACAGTTCATGGTCAGAAG+AGG - Chr1:5566209-5566228 MsG0180000404.01.T01:intron 40.0%
TGGTTGTGTAGGTATCAAAC+TGG - Chr1:5566985-5567004 MsG0180000404.01.T01:CDS 40.0%
TTCATGGTCAGAAGAGGATA+AGG - Chr1:5566215-5566234 MsG0180000404.01.T01:intron 40.0%
! GCTATGGAAACCATTGATTG+AGG - Chr1:5567028-5567047 MsG0180000404.01.T01:CDS 40.0%
! TGCACACTTTGCTGTCAAAA+CGG - Chr1:5566119-5566138 MsG0180000404.01.T01:CDS 40.0%
ATGAGAGCAGTGAAAGAAGC+AGG - Chr1:5567102-5567121 MsG0180000404.01.T01:CDS 45.0%
CTGTTTCCGTTCTACGTGAT+TGG - Chr1:5566340-5566359 MsG0180000404.01.T01:intron 45.0%
GGGCTCTTCAACAGTTGAAA+AGG - Chr1:5566274-5566293 MsG0180000404.01.T01:intron 45.0%
!! AAGTGGGTTGACTAGAAGTC+AGG - Chr1:5566655-5566674 MsG0180000404.01.T01:intron 45.0%
ATCAGTTATGGAGGCCCCAA+AGG - Chr1:5566301-5566320 MsG0180000404.01.T01:intron 50.0%
CGATTGCAGCCACAGTATCA+AGG - Chr1:5566442-5566461 MsG0180000404.01.T01:intron 50.0%
TAAATGCGCGTGTTCGGCTA+TGG - Chr1:5567012-5567031 MsG0180000404.01.T01:CDS 50.0%
TCAGTTATGGAGGCCCCAAA+GGG - Chr1:5566302-5566321 MsG0180000404.01.T01:intron 50.0%
! ATCTTTCTGGCAAGCCCCTT+TGG + Chr1:5566320-5566339 None:intergenic 50.0%
! TCTTTCTGGCAAGCCCCTTT+GGG + Chr1:5566319-5566338 None:intergenic 50.0%
AGCTGATGCAGCCGGAATTG+GGG + Chr1:5566476-5566495 None:intergenic 55.0%
CAGCTGATGCAGCCGGAATT+GGG + Chr1:5566477-5566496 None:intergenic 55.0%
CAGTTATGGAGGCCCCAAAG+GGG - Chr1:5566303-5566322 MsG0180000404.01.T01:intron 55.0%
CGAAATGCAGCTGATGCAGC+CGG + Chr1:5566484-5566503 None:intergenic 55.0%
! CTTTCTGGCAAGCCCCTTTG+GGG + Chr1:5566318-5566337 None:intergenic 55.0%
GCAGCTGATGCAGCCGGAAT+TGG + Chr1:5566478-5566497 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5566051 5567160 5566051 ID=MsG0180000404.01;Name=MsG0180000404.01
Chr1 mRNA 5566051 5567160 5566051 ID=MsG0180000404.01.T01;Parent=MsG0180000404.01;Name=MsG0180000404.01.T01;_AED=0.39;_eAED=0.41;_QI=0|0|0|1|1|1|3|0|187
Chr1 exon 5566823 5567160 5566823 ID=MsG0180000404.01.T01:exon:2699;Parent=MsG0180000404.01.T01
Chr1 exon 5566535 5566595 5566535 ID=MsG0180000404.01.T01:exon:2700;Parent=MsG0180000404.01.T01
Chr1 exon 5566051 5566215 5566051 ID=MsG0180000404.01.T01:exon:2701;Parent=MsG0180000404.01.T01
Chr1 CDS 5566823 5567160 5566823 ID=MsG0180000404.01.T01:cds;Parent=MsG0180000404.01.T01
Chr1 CDS 5566535 5566595 5566535 ID=MsG0180000404.01.T01:cds;Parent=MsG0180000404.01.T01
Chr1 CDS 5566051 5566215 5566051 ID=MsG0180000404.01.T01:cds;Parent=MsG0180000404.01.T01
Gene Sequence

>MsG0180000404.01.T01

ATGTTGGATGAGATTCACACTGTCGTATCAGCATTTCATGCTGCTACTGAGCTCGATCCACAAATACATGCACACTTTGCTGTCAAAACGGTTTCTCGACTATATAAGGACTTGAGAGAGAGGATTAGCAAACATATTCTTTCCATGGGATCAAATTTTAACAGTTCATGGTCAGAAGAGGATAAGGAATTGTCTGTTGAAACTTCATTCATTCAAAAGCAATGGGCTCTTCAACAGTTGAAAAGGAAAGATCAGTTATGGAGGCCCCAAAGGGGCTTGCCAGAAAGATCTGTTTCCGTTCTACGTGATTGGATGTTCAGAATTTCCTCCATCCGTAAGTATCCTAAAGATGCAGAGAAGCATTTACTTGCAATAAAAAGTGGGTTGACTAGAAGTCAGGTATCAAACTGGTTTATAAATGCGCGTGTTCGGCTATGGAAACCATTGATTGAGGAAATGTATGCTGAAATGAATAGAAGAAAAGCTTGTCGAAATGAAGGAGAAAATGAGAGCAGTGAAAGAAGCAGGATAAGCATTAACAATCAAATGTTTAATATGAATTGA

Protein sequence

>MsG0180000404.01.T01

MLDEIHTVVSAFHAATELDPQIHAHFAVKTVSRLYKDLRERISKHILSMGSNFNSSWSEEDKELSVETSFIQKQWALQQLKRKDQLWRPQRGLPERSVSVLRDWMFRISSIRKYPKDAEKHLLAIKSGLTRSQVSNWFINARVRLWKPLIEEMYAEMNRRKACRNEGENESSERSRISINNQMFNMN*