AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480022070.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480022070.01.T01 MTR_6g039060 31.950 482 258 13 24 468 19 467 1.53e-66 223
MsG0480022070.01.T01 MTR_5g049460 32.645 484 263 12 24 456 19 490 1.96e-63 213
MsG0480022070.01.T01 MTR_3g047490 37.126 334 163 6 1 298 1 323 5.45e-61 202
MsG0480022070.01.T01 MTR_0010s0130 33.772 456 244 15 24 463 19 432 4.79e-58 202
MsG0480022070.01.T01 MTR_0195s0040 27.961 515 306 12 14 468 13 522 1.40e-52 187
MsG0480022070.01.T01 MTR_0752s0020 30.266 489 259 16 1 463 1 433 6.83e-51 182
MsG0480022070.01.T01 MTR_0132s0050 33.809 491 241 17 1 461 1 437 1.65e-50 179
MsG0480022070.01.T01 MTR_6g052660 29.666 509 268 16 34 460 6 506 1.32e-49 179
MsG0480022070.01.T01 MTR_0387s0030 30.000 510 233 14 1 468 1 428 3.83e-47 173
MsG0480022070.01.T01 MTR_4g053915 29.688 512 277 12 26 468 25 522 2.09e-44 163
MsG0480022070.01.T01 MTR_6g464050 27.176 471 265 12 14 468 13 421 2.88e-40 155
MsG0480022070.01.T01 MTR_6g047410 29.353 402 223 12 26 373 24 418 1.07e-39 153
MsG0480022070.01.T01 MTR_7g068680 29.397 398 236 11 26 393 25 407 2.06e-39 147
MsG0480022070.01.T01 MTR_1465s0010 29.268 451 234 12 26 437 13 417 6.23e-39 147
MsG0480022070.01.T01 MTR_7g009270 27.644 539 298 16 14 468 13 543 1.01e-38 150
MsG0480022070.01.T01 MTR_4g046907 29.008 393 197 11 113 468 1 348 1.07e-38 147
MsG0480022070.01.T01 MTR_0547s0010 29.008 393 197 11 113 468 1 348 1.22e-38 146
MsG0480022070.01.T01 MTR_4g119430 31.111 315 174 7 26 301 24 334 1.36e-38 149
MsG0480022070.01.T01 MTR_5g084777 36.719 256 143 8 26 268 24 273 5.51e-38 148
MsG0480022070.01.T01 MTR_3g048350 30.030 333 187 8 34 328 6 330 9.53e-38 147
MsG0480022070.01.T01 MTR_7g099763 35.458 251 153 5 26 268 24 273 1.33e-37 140
MsG0480022070.01.T01 MTR_1g053665 31.765 340 202 10 80 409 24 343 1.74e-37 140
MsG0480022070.01.T01 MTR_8g012072 35.294 255 148 6 26 268 24 273 1.80e-37 146
MsG0480022070.01.T01 MTR_6g471790 35.294 255 148 6 26 268 24 273 1.86e-37 146
MsG0480022070.01.T01 MTR_8g087757 35.294 255 148 6 26 268 24 273 1.97e-37 146
MsG0480022070.01.T01 MTR_5g459570 35.294 255 148 6 26 268 24 273 2.00e-37 145
MsG0480022070.01.T01 MTR_3g436570 35.294 255 148 6 26 268 24 273 2.08e-37 146
MsG0480022070.01.T01 MTR_0133s0060 35.294 255 148 6 26 268 24 273 2.28e-37 146
MsG0480022070.01.T01 MTR_5g056945 34.252 254 146 5 1 242 1 245 4.83e-37 139
MsG0480022070.01.T01 MTR_7g024373 34.902 255 149 6 26 268 24 273 2.22e-36 143
MsG0480022070.01.T01 MTR_6g049080 28.609 381 187 9 24 374 19 344 1.68e-35 136
MsG0480022070.01.T01 MTR_0454s0020 29.006 362 206 8 36 349 147 505 1.85e-35 139
MsG0480022070.01.T01 MTR_0015s0160 30.063 316 174 6 26 300 25 334 3.61e-34 135
MsG0480022070.01.T01 MTR_6g033905 28.758 306 142 6 26 301 25 284 3.17e-30 124
MsG0480022070.01.T01 MTR_7g027027 40.341 176 96 5 26 193 24 198 2.58e-28 111
MsG0480022070.01.T01 MTR_0002s0140 43.796 137 73 1 24 156 19 155 1.86e-26 107
MsG0480022070.01.T01 MTR_8g468090 25.705 319 174 8 68 342 2 301 1.11e-23 104
MsG0480022070.01.T01 MTR_0322s0040 24.390 410 228 10 26 388 25 399 4.55e-23 101
MsG0480022070.01.T01 MTR_3g450160 30.508 177 117 2 72 242 1 177 3.22e-22 100
MsG0480022070.01.T01 MTR_7g038780 24.225 355 212 12 26 342 25 360 1.25e-20 94.7
MsG0480022070.01.T01 MTR_1g099290 27.941 272 153 7 62 321 320 560 1.73e-20 94.7
MsG0480022070.01.T01 MTR_5g056860 24.686 478 225 17 26 437 25 433 3.20e-18 88.2
MsG0480022070.01.T01 MTR_0324s0040 25.000 288 170 8 106 350 10 294 8.56e-17 82.0
MsG0480022070.01.T01 MTR_3g436840 48.980 98 47 2 1 98 1 95 1.97e-16 82.0
MsG0480022070.01.T01 MTR_7g045830 30.412 194 111 2 30 219 1 174 1.07e-13 73.2
MsG0480022070.01.T01 MTR_1g423950 43.836 73 41 0 26 98 24 96 1.24e-13 67.8
MsG0480022070.01.T01 MTR_3g040210 45.679 81 42 1 21 99 5 85 7.35e-13 70.5
MsG0480022070.01.T01 MTR_3g031890 42.683 82 43 3 26 104 25 105 1.74e-12 65.5
MsG0480022070.01.T01 MTR_3g047110 34.722 72 44 2 204 275 30 98 3.00e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 106 sgRNAs with CRISPR-Local

Find 129 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATAGTGCTTTCATTTA+TGG 0.020977 4:+67702453 None:intergenic
GGACTTTCCTCCCATCTTTA+AGG 0.153003 4:-67702204 MsG0480022070.01.T01:CDS
TGACTCTACTTGAACTATTA+AGG 0.226667 4:+67703059 None:intergenic
CTTCCTTCCTGACTATTTCT+TGG 0.228529 4:+67702707 None:intergenic
AAGCAAAGCGTATCAGTATC+TGG 0.259212 4:-67703123 MsG0480022070.01.T01:CDS
GCCTTCTTCTTCTTCAGCTT+GGG 0.265675 4:+67701792 None:intergenic
AGGTTACTACAACACTCTTT+TGG 0.268967 4:-67702671 MsG0480022070.01.T01:CDS
TGAACTTGGTCAAGGACTTT+TGG 0.305016 4:-67702930 MsG0480022070.01.T01:CDS
AATTTACTTCATGGCTATTC+TGG 0.318612 4:-67702521 MsG0480022070.01.T01:CDS
CTGCTGAAACTGAGATAATC+AGG 0.323360 4:-67702226 MsG0480022070.01.T01:CDS
TGATGCACCTAAAGGGAAAA+AGG 0.325899 4:-67702042 MsG0480022070.01.T01:CDS
GAGCTTCCTCCAGCTGCTCT+TGG 0.328304 4:+67701844 None:intergenic
ACGTCCTCAGTGATGGTTAT+AGG 0.334141 4:+67702769 None:intergenic
GAAGCTAGCAATTTACTTCA+TGG 0.334384 4:-67702530 MsG0480022070.01.T01:CDS
AAGAAAAGGTAAGGCAGATT+TGG 0.337872 4:-67701874 MsG0480022070.01.T01:CDS
CATTTGATAGCTTAGAAATA+AGG 0.347561 4:-67702810 MsG0480022070.01.T01:CDS
CTGGCCTTCTTTCCTTTAGT+AGG 0.350006 4:+67701990 None:intergenic
AGATGATCCATTACATATCT+TGG 0.352507 4:+67702484 None:intergenic
AGCCTTCTTCTTCTTCAGCT+TGG 0.352894 4:+67701791 None:intergenic
CCTGTTTCTATCAAGGAAAA+AGG 0.356420 4:-67701939 MsG0480022070.01.T01:CDS
TGCTAGCTTCTGTTGATTGT+TGG 0.363863 4:+67702543 None:intergenic
GAGTATTTCAAGATGCTAAA+TGG 0.364249 4:-67702964 MsG0480022070.01.T01:CDS
AGCTTCCTCCAGCTGCTCTT+GGG 0.372746 4:+67701845 None:intergenic
TTCTGACTCAGTACTAGTTC+TGG 0.378125 4:+67701971 None:intergenic
GCTAGCTTCTGTTGATTGTT+GGG 0.380746 4:+67702544 None:intergenic
GCTTCTGTTGATTGTTGGGT+TGG 0.399652 4:+67702548 None:intergenic
GGAGGAAGCTCTTGAAGAAA+TGG 0.399926 4:-67701832 MsG0480022070.01.T01:CDS
ACGTTATAGCTAAAGCTTGT+AGG 0.401395 4:-67702750 MsG0480022070.01.T01:CDS
AGAGTCACTTTCCTTAAAGA+TGG 0.416859 4:+67702193 None:intergenic
TTCTGACACAGCAACTGCTT+TGG 0.419983 4:+67702076 None:intergenic
CTTCTTCTTCAGCTTGGGAT+CGG 0.423630 4:+67701797 None:intergenic
AGATGCAAAGCCTTCTGAAA+TGG 0.433259 4:-67702638 MsG0480022070.01.T01:CDS
GAAGATTCCAAGAAATAGTC+AGG 0.435830 4:-67702714 MsG0480022070.01.T01:CDS
AATCTACAAAGAGAAGACTA+TGG 0.442901 4:-67703097 MsG0480022070.01.T01:CDS
CCTTCAACTTGTGAGTCCTA+TGG 0.444502 4:-67701766 MsG0480022070.01.T01:CDS
TGGTTGAGATGACTCCAATA+AGG 0.447143 4:-67701746 MsG0480022070.01.T01:CDS
GATGATCCATTACATATCTT+GGG 0.455203 4:+67702485 None:intergenic
GGAGGAAGATGCGAGGTCTC+AGG 0.460578 4:-67702135 MsG0480022070.01.T01:CDS
GAGGAAGCTGCTCTTGCTAA+AGG 0.460692 4:-67702113 MsG0480022070.01.T01:CDS
ATGTAATGGATCATCTCTGC+TGG 0.475466 4:-67702477 MsG0480022070.01.T01:CDS
AGAAATAAGGTCTGAAGTGA+TGG 0.475662 4:-67702797 MsG0480022070.01.T01:CDS
TGCTAAACCTGTTTCTATCA+AGG 0.477409 4:-67701946 MsG0480022070.01.T01:CDS
AAACTTCCCAAGATATGTAA+TGG 0.477975 4:-67702491 MsG0480022070.01.T01:CDS
AGGAAGCTGCTCTTGCTAAA+GGG 0.478652 4:-67702112 MsG0480022070.01.T01:CDS
ATCAGATTCAGAACTAGTTC+TGG 0.484699 4:+67702019 None:intergenic
GATTGCTTCTTTCAAAGAGC+AGG 0.488635 4:-67702580 MsG0480022070.01.T01:CDS
GGTAATCACCCAAGAGCAGC+TGG 0.489510 4:-67701853 MsG0480022070.01.T01:CDS
CTGTTTCTGATGCACCTAAA+GGG 0.489553 4:-67702049 MsG0480022070.01.T01:CDS
TGGACCAACATATGTGAACT+TGG 0.489582 4:-67702944 MsG0480022070.01.T01:CDS
TCTAAGCTATCAAATGATGT+AGG 0.490834 4:+67702817 None:intergenic
CTGAAATGGAGATTCACAAC+AGG 0.491040 4:-67702624 MsG0480022070.01.T01:CDS
AGATGACTCCAATAAGGAAA+AGG 0.494448 4:-67701740 MsG0480022070.01.T01:CDS
TCTGATGCTGCTCCTACTAA+AGG 0.495320 4:-67702002 MsG0480022070.01.T01:CDS
AGAAGACTATGGATATTCCA+TGG 0.500726 4:-67703086 MsG0480022070.01.T01:CDS
AAACAAGCTGGGTCAAAGAA+AGG 0.502080 4:-67702862 MsG0480022070.01.T01:CDS
AAAGAAAGGGAAAATGATCA+TGG 0.505028 4:-67702848 MsG0480022070.01.T01:CDS
GCTGTTTCTGATGCACCTAA+AGG 0.505921 4:-67702050 MsG0480022070.01.T01:CDS
AACAAGCTGGGTCAAAGAAA+GGG 0.513083 4:-67702861 MsG0480022070.01.T01:CDS
TGCTCCTACTAAAGGAAAGA+AGG 0.516416 4:-67701994 MsG0480022070.01.T01:CDS
TTGTGAATCTCCATTTCAGA+AGG 0.526263 4:+67702628 None:intergenic
ATCAGTAGCTGAGTACGTCT+TGG 0.528893 4:-67702156 MsG0480022070.01.T01:CDS
CATAGGACTCACAAGTTGAA+GGG 0.535256 4:+67701767 None:intergenic
AGGAAGAAACAAGTACCCTA+TGG 0.538949 4:-67702431 MsG0480022070.01.T01:CDS
GAGTCACTTTCCTTAAAGAT+GGG 0.539345 4:+67702194 None:intergenic
AGCAAAGCGTATCAGTATCT+GGG 0.540332 4:-67703122 MsG0480022070.01.T01:CDS
TTGACCAAGTTCACATATGT+TGG 0.542685 4:+67702940 None:intergenic
AAAGTCGTAAGCAAACAAGC+TGG 0.548529 4:-67702874 MsG0480022070.01.T01:CDS
TGTTGTAGTAACCTTGTAGC+AGG 0.549844 4:+67702680 None:intergenic
AGCTATAACGTCCTCAGTGA+TGG 0.550655 4:+67702762 None:intergenic
TTCAGACAGCAATCTTCCAT+AGG 0.552612 4:+67702415 None:intergenic
CCATAGGACTCACAAGTTGA+AGG 0.557869 4:+67701766 None:intergenic
AAGCCCCTAAAGATGAAAAG+TGG 0.558761 4:-67702262 MsG0480022070.01.T01:CDS
GAAATAAGGTCTGAAGTGAT+GGG 0.560325 4:-67702796 MsG0480022070.01.T01:CDS
ATTCCAAGAAATAGTCAGGA+AGG 0.562379 4:-67702710 MsG0480022070.01.T01:CDS
TGCTTCTTTCAAAGAGCAGG+AGG 0.563512 4:-67702577 MsG0480022070.01.T01:CDS
ATTGGAGTCATCTCAACCAT+AGG 0.564208 4:+67701750 None:intergenic
CAAGAAGTATGAAGTAAACA+TGG 0.567275 4:-67703016 MsG0480022070.01.T01:CDS
AAGGAAGATGTCCTGCTACA+AGG 0.568237 4:-67702691 MsG0480022070.01.T01:CDS
AGTAGCTGAGTACGTCTTGG+AGG 0.574153 4:-67702153 MsG0480022070.01.T01:CDS
TGAACTATTAAGGATTCCCA+TGG 0.576043 4:+67703069 None:intergenic
GCTGCTCTTGCTAAAGGGAA+AGG 0.581656 4:-67702107 MsG0480022070.01.T01:CDS
ACTCAGCTACTGATTGAACT+AGG 0.585948 4:+67702165 None:intergenic
TCAGACAGCAATCTTCCATA+GGG 0.588685 4:+67702416 None:intergenic
GGAGATTCACAACAGGATGT+TGG 0.588861 4:-67702617 MsG0480022070.01.T01:CDS
TGAAGAAATGGAGAAAGAAG+AGG 0.590995 4:-67701820 MsG0480022070.01.T01:CDS
AAGTCGTAAGCAAACAAGCT+GGG 0.591113 4:-67702873 MsG0480022070.01.T01:CDS
AAAGGAAAGATGCAGAAGGT+TGG 0.591290 4:-67701921 MsG0480022070.01.T01:CDS
AATAGTTCAAGTAGAGTCAC+CGG 0.592431 4:-67703055 MsG0480022070.01.T01:CDS
GCTTGTAGGATGAATGCAGA+GGG 0.594140 4:-67702736 MsG0480022070.01.T01:CDS
GACTCCAATAAGGAAAAGGA+TGG 0.600286 4:-67701736 MsG0480022070.01.T01:CDS
GAAGACTATGGATATTCCAT+GGG 0.608051 4:-67703085 MsG0480022070.01.T01:CDS
TCACTTTCCTTAAAGATGGG+AGG 0.608137 4:+67702197 None:intergenic
ATAAATGAAAGCACTATCAG+AGG 0.610449 4:-67702451 MsG0480022070.01.T01:CDS
AAGTATGAAGTAAACATGGA+AGG 0.610824 4:-67703012 MsG0480022070.01.T01:CDS
TGTAATGGATCATCTCTGCT+GGG 0.612052 4:-67702476 MsG0480022070.01.T01:CDS
TCCCAAGCTGAAGAAGAAGA+AGG 0.619996 4:-67701793 MsG0480022070.01.T01:CDS
TGCTGAAACTGAGATAATCA+GGG 0.625436 4:-67702225 MsG0480022070.01.T01:CDS
TTGAGTGAAGCAAAATCCAC+CGG 0.625573 4:+67703036 None:intergenic
GGAAGATGCGAGGTCTCAGG+AGG 0.628698 4:-67702132 MsG0480022070.01.T01:CDS
AACATATGTGAACTTGGTCA+AGG 0.631522 4:-67702938 MsG0480022070.01.T01:CDS
ATAGGACTCACAAGTTGAAG+GGG 0.642424 4:+67701768 None:intergenic
AATCACCCAAGAGCAGCTGG+AGG 0.697673 4:-67701850 MsG0480022070.01.T01:CDS
AGTTCAAGTAGAGTCACCGG+TGG 0.701435 4:-67703052 MsG0480022070.01.T01:CDS
ACGTCTTGGAGGAAGATGCG+AGG 0.711757 4:-67702142 MsG0480022070.01.T01:CDS
AGCTTGTAGGATGAATGCAG+AGG 0.721361 4:-67702737 MsG0480022070.01.T01:CDS
CATTCCTATAACCATCACTG+AGG 0.778544 4:-67702773 MsG0480022070.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTCCTCAAAAAAGAAGAAA+AGG - Chr4:67702978-67702997 MsG0480022070.01.T01:CDS 25.0%
! TCAAAAAAGAAGAAAAGGTA+AGG - Chr4:67702983-67703002 MsG0480022070.01.T01:CDS 25.0%
!! CATTTGATAGCTTAGAAATA+AGG - Chr4:67702056-67702075 MsG0480022070.01.T01:CDS 25.0%
!! TGAAATCTTTTATCAAGGAA+GGG - Chr4:67702470-67702489 MsG0480022070.01.T01:CDS 25.0%
!!! TTACCTTTTCTTCTTTTTTG+AGG + Chr4:67702984-67703003 None:intergenic 25.0%
AAACTTCCCAAGATATGTAA+TGG - Chr4:67702375-67702394 MsG0480022070.01.T01:intron 30.0%
AAAGAAAGGGAAAATGATCA+TGG - Chr4:67702018-67702037 MsG0480022070.01.T01:CDS 30.0%
AAGTATGAAGTAAACATGGA+AGG - Chr4:67701854-67701873 MsG0480022070.01.T01:CDS 30.0%
AATCTACAAAGAGAAGACTA+TGG - Chr4:67701769-67701788 MsG0480022070.01.T01:CDS 30.0%
AATTTACTTCATGGCTATTC+TGG - Chr4:67702345-67702364 MsG0480022070.01.T01:intron 30.0%
AGATGATCCATTACATATCT+TGG + Chr4:67702385-67702404 None:intergenic 30.0%
GAGTATTTCAAGATGCTAAA+TGG - Chr4:67701902-67701921 MsG0480022070.01.T01:CDS 30.0%
GATGATCCATTACATATCTT+GGG + Chr4:67702384-67702403 None:intergenic 30.0%
TCTAAGCTATCAAATGATGT+AGG + Chr4:67702052-67702071 None:intergenic 30.0%
TGACTCTACTTGAACTATTA+AGG + Chr4:67701810-67701829 None:intergenic 30.0%
! CAAGAAGTATGAAGTAAACA+TGG - Chr4:67701850-67701869 MsG0480022070.01.T01:CDS 30.0%
! CTGAAATCTTTTATCAAGGA+AGG - Chr4:67702469-67702488 MsG0480022070.01.T01:CDS 30.0%
! TCTGATAGTGCTTTCATTTA+TGG + Chr4:67702416-67702435 None:intergenic 30.0%
!! ATAAATGAAAGCACTATCAG+AGG - Chr4:67702415-67702434 MsG0480022070.01.T01:CDS 30.0%
!! CTAACCACTTTTCATCTTTA+GGG + Chr4:67702611-67702630 None:intergenic 30.0%
!! CTGTCTGAAATCTTTTATCA+AGG - Chr4:67702465-67702484 MsG0480022070.01.T01:CDS 30.0%
!! TAACCACTTTTCATCTTTAG+GGG + Chr4:67702610-67702629 None:intergenic 30.0%
!! TCTAACCACTTTTCATCTTT+AGG + Chr4:67702612-67702631 None:intergenic 30.0%
!!! TCTTCTTTTTTGAGGAATAG+AGG + Chr4:67702976-67702995 None:intergenic 30.0%
AACATATGTGAACTTGGTCA+AGG - Chr4:67701928-67701947 MsG0480022070.01.T01:CDS 35.0%
AAGAAAAGGTAAGGCAGATT+TGG - Chr4:67702992-67703011 MsG0480022070.01.T01:CDS 35.0%
AATAGTTCAAGTAGAGTCAC+CGG - Chr4:67701811-67701830 MsG0480022070.01.T01:CDS 35.0%
ACGTTATAGCTAAAGCTTGT+AGG - Chr4:67702116-67702135 MsG0480022070.01.T01:CDS 35.0%
AGAAGACTATGGATATTCCA+TGG - Chr4:67701780-67701799 MsG0480022070.01.T01:CDS 35.0%
AGATGACTCCAATAAGGAAA+AGG - Chr4:67703126-67703145 MsG0480022070.01.T01:CDS 35.0%
ATCAGATTCAGAACTAGTTC+TGG + Chr4:67702850-67702869 None:intergenic 35.0%
ATTCCAAGAAATAGTCAGGA+AGG - Chr4:67702156-67702175 MsG0480022070.01.T01:CDS 35.0%
GAAGACTATGGATATTCCAT+GGG - Chr4:67701781-67701800 MsG0480022070.01.T01:CDS 35.0%
GAAGATTCCAAGAAATAGTC+AGG - Chr4:67702152-67702171 MsG0480022070.01.T01:CDS 35.0%
GAAGCTAGCAATTTACTTCA+TGG - Chr4:67702336-67702355 MsG0480022070.01.T01:intron 35.0%
TGAACTATTAAGGATTCCCA+TGG + Chr4:67701800-67701819 None:intergenic 35.0%
TGAAGAAATGGAGAAAGAAG+AGG - Chr4:67703046-67703065 MsG0480022070.01.T01:CDS 35.0%
TGCTGAAACTGAGATAATCA+GGG - Chr4:67702641-67702660 MsG0480022070.01.T01:CDS 35.0%
TTGACCAAGTTCACATATGT+TGG + Chr4:67701929-67701948 None:intergenic 35.0%
TTGTGAATCTCCATTTCAGA+AGG + Chr4:67702241-67702260 None:intergenic 35.0%
! AGAAATAAGGTCTGAAGTGA+TGG - Chr4:67702069-67702088 MsG0480022070.01.T01:CDS 35.0%
! AGAGTCACTTTCCTTAAAGA+TGG + Chr4:67702676-67702695 None:intergenic 35.0%
! AGGTTACTACAACACTCTTT+TGG - Chr4:67702195-67702214 MsG0480022070.01.T01:CDS 35.0%
! CCTTTTTCCTTGATAGAAAC+AGG + Chr4:67702930-67702949 None:intergenic 35.0%
! GAAATAAGGTCTGAAGTGAT+GGG - Chr4:67702070-67702089 MsG0480022070.01.T01:CDS 35.0%
! GAGTCACTTTCCTTAAAGAT+GGG + Chr4:67702675-67702694 None:intergenic 35.0%
! GGTTACTACAACACTCTTTT+GGG - Chr4:67702196-67702215 MsG0480022070.01.T01:CDS 35.0%
!! CCTGTTTCTATCAAGGAAAA+AGG - Chr4:67702927-67702946 MsG0480022070.01.T01:CDS 35.0%
!! TGCTAAACCTGTTTCTATCA+AGG - Chr4:67702920-67702939 MsG0480022070.01.T01:CDS 35.0%
AAACAAGCTGGGTCAAAGAA+AGG - Chr4:67702004-67702023 MsG0480022070.01.T01:CDS 40.0%
AAAGGAAAGATGCAGAAGGT+TGG - Chr4:67702945-67702964 MsG0480022070.01.T01:CDS 40.0%
AAAGTCGTAAGCAAACAAGC+TGG - Chr4:67701992-67702011 MsG0480022070.01.T01:CDS 40.0%
AACAAGCTGGGTCAAAGAAA+GGG - Chr4:67702005-67702024 MsG0480022070.01.T01:CDS 40.0%
AAGCAAAGCGTATCAGTATC+TGG - Chr4:67701743-67701762 MsG0480022070.01.T01:CDS 40.0%
AAGCCCCTAAAGATGAAAAG+TGG - Chr4:67702604-67702623 MsG0480022070.01.T01:CDS 40.0%
AAGTCGTAAGCAAACAAGCT+GGG - Chr4:67701993-67702012 MsG0480022070.01.T01:CDS 40.0%
AGCAAAGCGTATCAGTATCT+GGG - Chr4:67701744-67701763 MsG0480022070.01.T01:CDS 40.0%
AGGAAGAAACAAGTACCCTA+TGG - Chr4:67702435-67702454 MsG0480022070.01.T01:CDS 40.0%
ATTGGAGTCATCTCAACCAT+AGG + Chr4:67703119-67703138 None:intergenic 40.0%
CATTCCTATAACCATCACTG+AGG - Chr4:67702093-67702112 MsG0480022070.01.T01:CDS 40.0%
CTGAAATGGAGATTCACAAC+AGG - Chr4:67702242-67702261 MsG0480022070.01.T01:CDS 40.0%
CTGCTGAAACTGAGATAATC+AGG - Chr4:67702640-67702659 MsG0480022070.01.T01:CDS 40.0%
GACTCCAATAAGGAAAAGGA+TGG - Chr4:67703130-67703149 MsG0480022070.01.T01:CDS 40.0%
GATTGCTTCTTTCAAAGAGC+AGG - Chr4:67702286-67702305 MsG0480022070.01.T01:CDS 40.0%
GGAAAAAGGAAAGATGCAGA+AGG - Chr4:67702941-67702960 MsG0480022070.01.T01:CDS 40.0%
TCACTTTCCTTAAAGATGGG+AGG + Chr4:67702672-67702691 None:intergenic 40.0%
TCAGACAGCAATCTTCCATA+GGG + Chr4:67702453-67702472 None:intergenic 40.0%
TGATGCACCTAAAGGGAAAA+AGG - Chr4:67702824-67702843 MsG0480022070.01.T01:CDS 40.0%
TGCTCCTACTAAAGGAAAGA+AGG - Chr4:67702872-67702891 MsG0480022070.01.T01:CDS 40.0%
TGGACCAACATATGTGAACT+TGG - Chr4:67701922-67701941 MsG0480022070.01.T01:CDS 40.0%
TGGAGTTCTTAAGAAGGACA+AGG - Chr4:67702496-67702515 MsG0480022070.01.T01:CDS 40.0%
TGGTTGAGATGACTCCAATA+AGG - Chr4:67703120-67703139 MsG0480022070.01.T01:CDS 40.0%
TGTTGTAGTAACCTTGTAGC+AGG + Chr4:67702189-67702208 None:intergenic 40.0%
TTCAGACAGCAATCTTCCAT+AGG + Chr4:67702454-67702473 None:intergenic 40.0%
TTCTGACTCAGTACTAGTTC+TGG + Chr4:67702898-67702917 None:intergenic 40.0%
TTGAGTGAAGCAAAATCCAC+CGG + Chr4:67701833-67701852 None:intergenic 40.0%
! ACTCAGCTACTGATTGAACT+AGG + Chr4:67702704-67702723 None:intergenic 40.0%
! AGATGCAAAGCCTTCTGAAA+TGG - Chr4:67702228-67702247 MsG0480022070.01.T01:CDS 40.0%
! ATAGGACTCACAAGTTGAAG+GGG + Chr4:67703101-67703120 None:intergenic 40.0%
! CATAGGACTCACAAGTTGAA+GGG + Chr4:67703102-67703121 None:intergenic 40.0%
! CTTCCTTCCTGACTATTTCT+TGG + Chr4:67702162-67702181 None:intergenic 40.0%
! GCTAGCTTCTGTTGATTGTT+GGG + Chr4:67702325-67702344 None:intergenic 40.0%
! TACTACAACACTCTTTTGGG+TGG - Chr4:67702199-67702218 MsG0480022070.01.T01:CDS 40.0%
! TCTGCCATCCTTTTCCTTAT+TGG + Chr4:67703137-67703156 None:intergenic 40.0%
! TGAACTTGGTCAAGGACTTT+TGG - Chr4:67701936-67701955 MsG0480022070.01.T01:CDS 40.0%
! TGCTAGCTTCTGTTGATTGT+TGG + Chr4:67702326-67702345 None:intergenic 40.0%
! TGTTTGCTTACGACTTTTGC+AGG + Chr4:67701989-67702008 None:intergenic 40.0%
!! ACTACAACACTCTTTTGGGT+GGG - Chr4:67702200-67702219 MsG0480022070.01.T01:CDS 40.0%
!! ATGTAATGGATCATCTCTGC+TGG - Chr4:67702389-67702408 MsG0480022070.01.T01:intron 40.0%
!! CTGTTTCTGATGCACCTAAA+GGG - Chr4:67702817-67702836 MsG0480022070.01.T01:CDS 40.0%
!! CTTTTATCAAGGAAGGGTAC+TGG - Chr4:67702476-67702495 MsG0480022070.01.T01:CDS 40.0%
!! TGTAATGGATCATCTCTGCT+GGG - Chr4:67702390-67702409 MsG0480022070.01.T01:intron 40.0%
AAGGAAGATGTCCTGCTACA+AGG - Chr4:67702175-67702194 MsG0480022070.01.T01:CDS 45.0%
ACGTCCTCAGTGATGGTTAT+AGG + Chr4:67702100-67702119 None:intergenic 45.0%
AGCCTTCTTCTTCTTCAGCT+TGG + Chr4:67703078-67703097 None:intergenic 45.0%
AGCTATAACGTCCTCAGTGA+TGG + Chr4:67702107-67702126 None:intergenic 45.0%
AGCTTGTAGGATGAATGCAG+AGG - Chr4:67702129-67702148 MsG0480022070.01.T01:CDS 45.0%
AGGAAGCTGCTCTTGCTAAA+GGG - Chr4:67702754-67702773 MsG0480022070.01.T01:CDS 45.0%
ATCAGTAGCTGAGTACGTCT+TGG - Chr4:67702710-67702729 MsG0480022070.01.T01:CDS 45.0%
CCTTCAACTTGTGAGTCCTA+TGG - Chr4:67703100-67703119 MsG0480022070.01.T01:CDS 45.0%
CTGAAAGGACTATCAGTAGC+AGG - Chr4:67702553-67702572 MsG0480022070.01.T01:CDS 45.0%
CTTCTTCTTCAGCTTGGGAT+CGG + Chr4:67703072-67703091 None:intergenic 45.0%
GCCTTCTTCTTCTTCAGCTT+GGG + Chr4:67703077-67703096 None:intergenic 45.0%
GCTTGTAGGATGAATGCAGA+GGG - Chr4:67702130-67702149 MsG0480022070.01.T01:CDS 45.0%
GGACTTTCCTCCCATCTTTA+AGG - Chr4:67702662-67702681 MsG0480022070.01.T01:CDS 45.0%
GGAGATTCACAACAGGATGT+TGG - Chr4:67702249-67702268 MsG0480022070.01.T01:CDS 45.0%
GGAGGAAGCTCTTGAAGAAA+TGG - Chr4:67703034-67703053 MsG0480022070.01.T01:CDS 45.0%
TCCCAAGCTGAAGAAGAAGA+AGG - Chr4:67703073-67703092 MsG0480022070.01.T01:CDS 45.0%
TCGAAGTCTCTACTGCTGAA+AGG - Chr4:67702538-67702557 MsG0480022070.01.T01:CDS 45.0%
TCTGATGCTGCTCCTACTAA+AGG - Chr4:67702864-67702883 MsG0480022070.01.T01:CDS 45.0%
TGCTTCTTTCAAAGAGCAGG+AGG - Chr4:67702289-67702308 MsG0480022070.01.T01:CDS 45.0%
TTCTGACACAGCAACTGCTT+TGG + Chr4:67702793-67702812 None:intergenic 45.0%
! CCATAGGACTCACAAGTTGA+AGG + Chr4:67703103-67703122 None:intergenic 45.0%
! CTGGCCTTCTTTCCTTTAGT+AGG + Chr4:67702879-67702898 None:intergenic 45.0%
! GCTTCTGTTGATTGTTGGGT+TGG + Chr4:67702321-67702340 None:intergenic 45.0%
!! GCTGTTTCTGATGCACCTAA+AGG - Chr4:67702816-67702835 MsG0480022070.01.T01:CDS 45.0%
!! GGGTACTGGAGTTCTTAAGA+AGG - Chr4:67702490-67702509 MsG0480022070.01.T01:CDS 45.0%
!! GTTCTGGCCTTTTTCCCTTT+AGG + Chr4:67702834-67702853 None:intergenic 45.0%
AGTAGCTGAGTACGTCTTGG+AGG - Chr4:67702713-67702732 MsG0480022070.01.T01:CDS 50.0%
AGTTCAAGTAGAGTCACCGG+TGG - Chr4:67701814-67701833 MsG0480022070.01.T01:CDS 50.0%
GAGGAAGCTGCTCTTGCTAA+AGG - Chr4:67702753-67702772 MsG0480022070.01.T01:CDS 50.0%
GCTGCTCTTGCTAAAGGGAA+AGG - Chr4:67702759-67702778 MsG0480022070.01.T01:CDS 50.0%
AATCACCCAAGAGCAGCTGG+AGG - Chr4:67703016-67703035 MsG0480022070.01.T01:CDS 55.0%
ACGTCTTGGAGGAAGATGCG+AGG - Chr4:67702724-67702743 MsG0480022070.01.T01:CDS 55.0%
AGCTTCCTCCAGCTGCTCTT+GGG + Chr4:67703024-67703043 None:intergenic 55.0%
GGTAATCACCCAAGAGCAGC+TGG - Chr4:67703013-67703032 MsG0480022070.01.T01:CDS 55.0%
GAGCTTCCTCCAGCTGCTCT+TGG + Chr4:67703025-67703044 None:intergenic 60.0%
GGAAGATGCGAGGTCTCAGG+AGG - Chr4:67702734-67702753 MsG0480022070.01.T01:CDS 60.0%
GGAGGAAGATGCGAGGTCTC+AGG - Chr4:67702731-67702750 MsG0480022070.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr4 gene 67701701 67703187 67701701 ID=MsG0480022070.01;Name=MsG0480022070.01
Chr4 mRNA 67701701 67703187 67701701 ID=MsG0480022070.01.T01;Parent=MsG0480022070.01;Name=MsG0480022070.01.T01;_AED=0.53;_eAED=1.00;_QI=0|0|0|1|0|0|2|0|468
Chr4 exon 67702397 67703187 67702397 ID=MsG0480022070.01.T01:exon:18384;Parent=MsG0480022070.01.T01
Chr4 exon 67701701 67702313 67701701 ID=MsG0480022070.01.T01:exon:18383;Parent=MsG0480022070.01.T01
Chr4 CDS 67702397 67703187 67702397 ID=MsG0480022070.01.T01:cds;Parent=MsG0480022070.01.T01
Chr4 CDS 67701701 67702313 67701701 ID=MsG0480022070.01.T01:cds;Parent=MsG0480022070.01.T01
Gene Sequence

>MsG0480022070.01.T01

ATGGCATCATCAAGCTCAAAAGTTTCAGAATCTACATCAAACAAGCAAAGCGTATCAGTATCTGGGTCAATCTACAAAGAGAAGACTATGGATATTCCATGGGAATCCTTAATAGTTCAAGTAGAGTCACCGGTGGATTTTGCTTCACTCAAGAAGTATGAAGTAAACATGGAAGGATTGATAGAGACTCAGATGCTGTCTGAGTATTTCAAGATGCTAAATGGACCAACATATGTGAACTTGGTCAAGGACTTTTGGTTAAAAGCTGAAGTGTACAATGAAAAGCCTGCAAAAGTCGTAAGCAAACAAGCTGGGTCAAAGAAAGGGAAAATGATCATGGATATGAATCCTACATCATTTGATAGCTTAGAAATAAGGTCTGAAGTGATGGGCATTCCTATAACCATCACTGAGGACGTTATAGCTAAAGCTTGTAGGATGAATGCAGAGGGAAGATTCCAAGAAATAGTCAGGAAGGAAGATGTCCTGCTACAAGGTTACTACAACACTCTTTTGGGTGGGAAGAAAGATGCAAAGCCTTCTGAAATGGAGATTCACAACAGGATGTTGGTGAAATTCAGTAATGATTGCTTCTTTCAAAGAGCAGGAGGATCTGACCAACCCAACAATCAACAGAAGCTAGCAATTTACTTCATGGCTATTCTGGAAAAGATAAACTTCCCAAGATATGTAATGGATCATCTCTGCTGGGCCATAAATGAAAGCACTATCAGAGGAAGAAACAAGTACCCTATGGAAGATTGCTGTCTGAAATCTTTTATCAAGGAAGGGAGCTTATGCTCTATGAAGTTCATCAAAGAAGCCCCTAAAGATGAAAAGTGGTTAGAGATGACAACTGCTGAAACTGAGATAATCAGGGACTTTCCTCCCATCTTTAAGGAAAGTGACTCTGATTTCCTAGTTCAATCAGTAGCTGAGTACGTCTTGGAGGAAGATGCGAGGTCTCAGGAGGAAGCTGCTCTTGCTAAAGGGAAAGGCAAGAAGACCAAAGCAGTTGCTGTGTCAGAAGCTGCTGTTTCTGATGCACCTAAAGGGAAAAAGGCCAGAACTAGTTCTGAATCTGATGCTGCTCCTACTAAAGGAAAGAAGGCCAGAACTAGTACTGAGTCAGAAGCTGCTAAACCTGTTTCTATCAAGGAAAAAGGAAAGATGCAGAAGGTTGGTGCTGCCTCTATTCCTCAAAAAAGAAGAAAAGGTAAGGCAGATTTGGTAATCACCCAAGAGCAGCTGGAGGAAGCTCTTGAAGAAATGGAGAAAGAAGAGGCCGATCCCAAGCTGAAGAAGAAGAAGGCTCCCCTTCAACTTGTGAGTCCTATGGTTGAGATGACTCCAATAAGGAAAAGGATGGCAGATGAACGTGCTTCTGATCTTATATCAGACAGA
Protein sequence

>MsG0480022070.01.T01

MASSSSKVSESTSNKQSVSVSGSIYKEKTMDIPWESLIVQVESPVDFASLKKYEVNMEGLIETQMLSEYFKMLNGPTYVNLVKDFWLKAEVYNEKPAKVVSKQAGSKKGKMIMDMNPTSFDSLEIRSEVMGIPITITEDVIAKACRMNAEGRFQEIVRKEDVLLQGYYNTLLGGKKDAKPSEMEIHNRMLVKFSNDCFFQRAGGSDQPNNQQKLAIYFMAILEKINFPRYVMDHLCWAINESTIRGRNKYPMEDCCLKSFIKEGSLCSMKFIKEAPKDEKWLEMTTAETEIIRDFPPIFKESDSDFLVQSVAEYVLEEDARSQEEAALAKGKGKKTKAVAVSEAAVSDAPKGKKARTSSESDAAPTKGKKARTSTESEAAKPVSIKEKGKMQKVGAASIPQKRRKGKADLVITQEQLEEALEEMEKEEADPKLKKKKAPLQLVSPMVEMTPIRKRMADERASDLISDR