AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049033.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049033.01.T01 MTR_4g134640 95.798 357 15 0 1 357 1 357 0.0 646
MsG0080049033.01.T01 MTR_4g134640 94.909 275 14 0 83 357 3 277 3.77e-173 496
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049033.01.T01 AT5G49880 54.278 374 153 4 1 357 1 373 6.36e-124 373

Find 81 sgRNAs with CRISPR-Local

Find 190 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCGAAGGTTTGTCTCATTT+TGG 0.238880 contig621end:+24076 None:intergenic
CGATCATTCACAACTTAAAA+AGG 0.261097 contig621end:-23944 MsG0080049033.01.T01:CDS
TTCTACAGGGAGTGATGTTT+TGG 0.261658 contig621end:-23543 MsG0080049033.01.T01:CDS
AAGTTGCGCCTTTGGGGTTT+GGG 0.269381 contig621end:-24747 MsG0080049033.01.T01:five_prime_UTR
AAAGTTGCGCCTTTGGGGTT+TGG 0.300808 contig621end:-24748 MsG0080049033.01.T01:five_prime_UTR
TGAATAAAAGTTGCGCCTTT+GGG 0.302332 contig621end:-24754 MsG0080049033.01.T01:five_prime_UTR
GGAAATTATGATTGAGTGTT+TGG 0.313529 contig621end:+24357 None:intergenic
TTGAATAAAAGTTGCGCCTT+TGG 0.316012 contig621end:-24755 MsG0080049033.01.T01:five_prime_UTR
GACAAAGCAACATCTTTAGA+AGG 0.333909 contig621end:-24021 MsG0080049033.01.T01:CDS
CTAAAGATGTTGCTTTGTCT+TGG 0.341234 contig621end:+24025 None:intergenic
CATTGCAAACCTCTTTGAAT+TGG 0.351624 contig621end:+23728 None:intergenic
TCAATTAAACACTGAACTTC+AGG 0.351637 contig621end:-24106 MsG0080049033.01.T01:CDS
GCTCAGATTGTGCCCTTTCC+CGG 0.364509 contig621end:+22244 None:intergenic
CTTGTTGTAGCTTGAACAAA+TGG 0.366662 contig621end:-23818 MsG0080049033.01.T01:intron
TCCCAGGAAGTTTCTTCTAC+AGG 0.379616 contig621end:-23557 MsG0080049033.01.T01:CDS
TAAAGATGTTGCTTTGTCTT+GGG 0.400406 contig621end:+24026 None:intergenic
TCCCTGTAGAAGAAACTTCC+TGG 0.401242 contig621end:+23555 None:intergenic
CTTTGAATTGGTCCTTAAGA+TGG 0.406182 contig621end:+23740 None:intergenic
TCATCGGAACGTGGCCTCTT+CGG 0.417235 contig621end:+24646 None:intergenic
TGCTTTGTCTTGGGCGGAAG+CGG 0.418194 contig621end:+24035 None:intergenic
TTTGGTTAAACAATTGCAAC+AGG 0.422570 contig621end:-23525 MsG0080049033.01.T01:CDS
AGAGCAACTGGACCATCTTA+AGG 0.435575 contig621end:-23752 MsG0080049033.01.T01:CDS
TGCGTCGCCGTCGCCGTCAT+CGG 0.440741 contig621end:+24630 None:intergenic
GGATCCGGCGGCAGCGGTGG+AGG 0.459538 contig621end:-24590 MsG0080049033.01.T01:CDS
AGACCTGCTTAAAGAGCAAC+TGG 0.463462 contig621end:-23764 MsG0080049033.01.T01:CDS
TGCTGGAGGAAAAGAGCTGC+CGG 0.470660 contig621end:-22263 MsG0080049033.01.T01:CDS
CAAGAACTCGCTGCTTCTAA+AGG 0.472308 contig621end:-24201 MsG0080049033.01.T01:CDS
TCTTCGGTGGCGGAGGAGTA+CGG 0.473231 contig621end:+24662 None:intergenic
CGTGGCCTCTTCGGTGGCGG+AGG 0.474010 contig621end:+24655 None:intergenic
ACTCCAGGATCAACTCTTGA+AGG 0.475492 contig621end:-24166 MsG0080049033.01.T01:CDS
AGGAAAAGAGCTGCCGGGAA+AGG 0.476342 contig621end:-22257 MsG0080049033.01.T01:CDS
AAGCAGGTCTGCTTCTCTCT+CGG 0.477842 contig621end:+23777 None:intergenic
GATTCTAGAAATAGAAAAGT+TGG 0.479879 contig621end:+23918 None:intergenic
TGTAAAGCTGCAAATTTAGC+AGG 0.480888 contig621end:+22111 None:intergenic
CACATATCAGTGTCGCCAAA+TGG 0.482171 contig621end:-24545 MsG0080049033.01.T01:intron
AGTTGCGCCTTTGGGGTTTG+GGG 0.482750 contig621end:-24746 MsG0080049033.01.T01:five_prime_UTR
CTATGAGGATCCGGCGGCAG+CGG 0.485300 contig621end:-24596 MsG0080049033.01.T01:CDS
GGAAAAGAGCTGCCGGGAAA+GGG 0.488404 contig621end:-22256 MsG0080049033.01.T01:CDS
TGCTTCTAAAGGCCGTGAAA+AGG 0.491539 contig621end:-24190 MsG0080049033.01.T01:CDS
GGTTCCTCCACCGCTGCCGC+CGG 0.494163 contig621end:+24586 None:intergenic
ATCTGAGCTATCTAAACTAC+AGG 0.495023 contig621end:-22229 MsG0080049033.01.T01:CDS
TGAGGATCCGGCGGCAGCGG+TGG 0.495529 contig621end:-24593 MsG0080049033.01.T01:CDS
GGTCCAGTTGCTCTTTAAGC+AGG 0.500008 contig621end:+23761 None:intergenic
CCCAGGAAGTTTCTTCTACA+GGG 0.507888 contig621end:-23556 MsG0080049033.01.T01:CDS
GAACGTGGCCTCTTCGGTGG+CGG 0.508356 contig621end:+24652 None:intergenic
GCTTGTTATCTATGAGGATC+CGG 0.510386 contig621end:-24605 MsG0080049033.01.T01:CDS
AGGCCGTGAAAAGGCACTCC+AGG 0.512926 contig621end:-24181 MsG0080049033.01.T01:CDS
TCTCTATGGTCTCTGAAAGA+TGG 0.530121 contig621end:+23989 None:intergenic
GGCGGAAGCGGCATGAGACT+CGG 0.530624 contig621end:+24047 None:intergenic
CGCACAGCTTGTTATCTATG+AGG 0.533714 contig621end:-24611 MsG0080049033.01.T01:CDS
ACCTGGCATCTCATGTTTCG+AGG 0.534847 contig621end:-22139 MsG0080049033.01.T01:CDS
CCTCCTTCAAGAGTTGATCC+TGG 0.535275 contig621end:+24163 None:intergenic
ACATTGACAACTTACATTAG+CGG 0.540144 contig621end:+23894 None:intergenic
AAGAGGCCACGTTCCGATGA+CGG 0.543240 contig621end:-24643 MsG0080049033.01.T01:CDS
TCCTCGAAACATGAGATGCC+AGG 0.545495 contig621end:+22138 None:intergenic
CCCTGTAGAAGAAACTTCCT+GGG 0.564281 contig621end:+23556 None:intergenic
TAGAGGATCAAATATCATCA+TGG 0.572164 contig621end:-22182 MsG0080049033.01.T01:CDS
ACATCTTTAGAAGGGAAGCT+AGG 0.573337 contig621end:-24012 MsG0080049033.01.T01:CDS
TCGGAACGTGGCCTCTTCGG+TGG 0.574434 contig621end:+24649 None:intergenic
GAATAAAAGTTGCGCCTTTG+GGG 0.579802 contig621end:-24753 MsG0080049033.01.T01:five_prime_UTR
GTGCATAGCATGTGCTCGGA+GGG 0.581042 contig621end:+24565 None:intergenic
GTACTCCTCCGCCACCGAAG+AGG 0.584759 contig621end:-24660 MsG0080049033.01.T01:CDS
ACAAAGCAACATCTTTAGAA+GGG 0.593342 contig621end:-24020 MsG0080049033.01.T01:CDS
GCTGGAGGAAAAGAGCTGCC+GGG 0.594033 contig621end:-22262 MsG0080049033.01.T01:CDS
TCTTAAGGACCAATTCAAAG+AGG 0.599055 contig621end:-23737 MsG0080049033.01.T01:intron
CCGTCGCCGTCATCGGAACG+TGG 0.606794 contig621end:+24637 None:intergenic
GAGACTCGGCTTGTATGCGA+AGG 0.619176 contig621end:+24061 None:intergenic
CCACGTTCCGATGACGGCGA+CGG 0.626939 contig621end:-24637 MsG0080049033.01.T01:CDS
AGATGTTGCTTTGTCTTGGG+CGG 0.631632 contig621end:+24029 None:intergenic
AAATATTATATTGTGAAAGG+AGG 0.636195 contig621end:-24794 MsG0080049033.01.T01:five_prime_UTR
ATATGTGCATAGCATGTGCT+CGG 0.645443 contig621end:+24561 None:intergenic
CACTGAGAGGAAGAAATCTG+TGG 0.647981 contig621end:-24244 MsG0080049033.01.T01:CDS
TTGAAATCCCCAAACCCCAA+AGG 0.648069 contig621end:+24739 None:intergenic
TTTGAAGGAGAAACTGCTGG+AGG 0.658729 contig621end:-22277 MsG0080049033.01.T01:CDS
TGTGCATAGCATGTGCTCGG+AGG 0.667920 contig621end:+24564 None:intergenic
TCTTTCAGAGACCATAGAGA+GGG 0.673302 contig621end:-23986 MsG0080049033.01.T01:CDS
ATGACATGACAGACACTGAG+AGG 0.677214 contig621end:-24257 MsG0080049033.01.T01:intron
CCAGGATCAACTCTTGAAGG+AGG 0.683215 contig621end:-24163 MsG0080049033.01.T01:CDS
TGTTATCTATGAGGATCCGG+CGG 0.704459 contig621end:-24602 MsG0080049033.01.T01:CDS
ATCTTTCAGAGACCATAGAG+AGG 0.704981 contig621end:-23987 MsG0080049033.01.T01:CDS
TTGATGATTAAAGACATACC+TGG 0.713365 contig621end:-22156 MsG0080049033.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATTATTTTTATTTTGTGTT+AGG - contig621end:23558-23577 MsG0080049033.01.T01:CDS 10.0%
!!! TTTATTTTCTTAAATCTTTT+GGG - contig621end:23656-23675 MsG0080049033.01.T01:intron 10.0%
!!! TTTTATTTTCTTAAATCTTT+TGG - contig621end:23655-23674 MsG0080049033.01.T01:intron 10.0%
!! ATAAAGTGAAAAAAAATCAA+TGG + contig621end:23810-23829 None:intergenic 15.0%
!! CAAAAATATTATATTGTGAA+AGG - contig621end:22096-22115 MsG0080049033.01.T01:CDS 15.0%
!! TAATAATAATAATAAGAGAC+AGG + contig621end:22417-22436 None:intergenic 15.0%
!!! CACATTATTGTTTTATTAAA+TGG - contig621end:23581-23600 MsG0080049033.01.T01:CDS 15.0%
!!! CTAACTTTTATTTTTTTTAG+GGG - contig621end:24415-24434 MsG0080049033.01.T01:CDS 15.0%
!!! GCTAACTTTTATTTTTTTTA+GGG - contig621end:24414-24433 MsG0080049033.01.T01:CDS 15.0%
!! AAAAATCAATGGTATCTTTA+TGG + contig621end:23799-23818 None:intergenic 20.0%
!! AAATATTATATTGTGAAAGG+AGG - contig621end:22099-22118 MsG0080049033.01.T01:CDS 20.0%
!! AATCATAATTTCCAAAAACT+CGG - contig621end:22546-22565 MsG0080049033.01.T01:intron 20.0%
!! ATATAAGTTAAATTCATCCA+TGG - contig621end:24040-24059 MsG0080049033.01.T01:CDS 20.0%
!! GAATAGCAATTATTTGTTTA+GGG - contig621end:24349-24368 MsG0080049033.01.T01:CDS 20.0%
!! TGAATAGCAATTATTTGTTT+AGG - contig621end:24348-24367 MsG0080049033.01.T01:CDS 20.0%
!!! AATCTTTTGGGATATTTTTT+TGG - contig621end:23668-23687 MsG0080049033.01.T01:intron 20.0%
!!! GGCTAACTTTTATTTTTTTT+AGG - contig621end:24413-24432 MsG0080049033.01.T01:CDS 20.0%
!!! GTTTCTATTATTATGAGTTT+TGG - contig621end:24162-24181 MsG0080049033.01.T01:CDS 20.0%
!!! TAATTTTGCAGGATTAAATT+AGG - contig621end:24392-24411 MsG0080049033.01.T01:CDS 20.0%
!!! TATTGTTTTATTAAATGGTC+TGG - contig621end:23586-23605 MsG0080049033.01.T01:CDS 20.0%
!!! TTCATGTTTCTTTTTTTAGT+GGG - contig621end:23218-23237 MsG0080049033.01.T01:intron 20.0%
!!! TTTCATGTTTCTTTTTTTAG+TGG - contig621end:23217-23236 MsG0080049033.01.T01:intron 20.0%
! AAAATAAAAAGACACGATTC+AGG + contig621end:23644-23663 None:intergenic 25.0%
! AGAATGATTGATAGATATGA+AGG + contig621end:23038-23057 None:intergenic 25.0%
! GTCAAATATGAAAAAGTTAG+AGG - contig621end:24694-24713 MsG0080049033.01.T01:five_prime_UTR 25.0%
! TAAATTTGACACCTGATATT+TGG - contig621end:23769-23788 MsG0080049033.01.T01:CDS 25.0%
! TCTCATATTAGATTGTGTTA+TGG - contig621end:24323-24342 MsG0080049033.01.T01:intron 25.0%
! TTAATCCTGCAAAATTAACT+TGG + contig621end:24389-24408 None:intergenic 25.0%
! TTTATGGTATTCCAAATATC+AGG + contig621end:23783-23802 None:intergenic 25.0%
!! AAAAACGCAGTCATATATTT+TGG + contig621end:23701-23720 None:intergenic 25.0%
!! GATTCTAGAAATAGAAAAGT+TGG + contig621end:22978-22997 None:intergenic 25.0%
!! TTCTTCTTCCTTTTCTTTTA+GGG - contig621end:23840-23859 MsG0080049033.01.T01:intron 25.0%
!! TTGATTTTCTGCTTTACTTA+AGG + contig621end:22493-22512 None:intergenic 25.0%
!! TTTCTTCTTCCTTTTCTTTT+AGG - contig621end:23839-23858 MsG0080049033.01.T01:intron 25.0%
!!! AATTTTAGGAATCTGAAGTA+AGG - contig621end:24552-24571 MsG0080049033.01.T01:CDS 25.0%
!!! ACTTTTATTTTTTTTAGGGG+AGG - contig621end:24418-24437 MsG0080049033.01.T01:CDS 25.0%
!!! ATCTTTTGATTGTTTGTGTA+AGG - contig621end:23448-23467 MsG0080049033.01.T01:intron 25.0%
!!! ATTTTAGGAATCTGAAGTAA+GGG - contig621end:24553-24572 MsG0080049033.01.T01:CDS 25.0%
!!! CTAACTTTTTCATATTTGAC+TGG + contig621end:24694-24713 None:intergenic 25.0%
!!! CTTTTATTTTTTTTAGGGGA+GGG - contig621end:24419-24438 MsG0080049033.01.T01:CDS 25.0%
!!! TTTTATTTTTTTTAGGGGAG+GGG - contig621end:24420-24439 MsG0080049033.01.T01:CDS 25.0%
!!! TTTTCTAAAACTACATGCTT+GGG - contig621end:24017-24036 MsG0080049033.01.T01:CDS 25.0%
!!! TTTTTCTAAAACTACATGCT+TGG - contig621end:24016-24035 MsG0080049033.01.T01:CDS 25.0%
AAACAAACAAACCAACCATT+TGG + contig621end:22366-22385 None:intergenic 30.0%
ACATTGACAACTTACATTAG+CGG + contig621end:23002-23021 None:intergenic 30.0%
ATATAATAGATGCAACGTTC+AGG + contig621end:24084-24103 None:intergenic 30.0%
CAAACAATCAAAAGATCAAG+AGG + contig621end:23444-23463 None:intergenic 30.0%
CCAAAAGTGAATTTAACGTT+TGG + contig621end:24188-24207 None:intergenic 30.0%
CCTAAAATTGCAAAGTTAAC+TGG + contig621end:24541-24560 None:intergenic 30.0%
CGATCATTCACAACTTAAAA+AGG - contig621end:22949-22968 MsG0080049033.01.T01:intron 30.0%
GGAAATTATGATTGAGTGTT+TGG + contig621end:22539-22558 None:intergenic 30.0%
GGATATATAAGATGAGGTTT+GGG - contig621end:23607-23626 MsG0080049033.01.T01:CDS 30.0%
TACATTACTCAGATTACTAC+AGG + contig621end:24517-24536 None:intergenic 30.0%
TAGAGGATCAAATATCATCA+TGG - contig621end:24711-24730 MsG0080049033.01.T01:five_prime_UTR 30.0%
TCAAGTGACATGATCATAAA+TGG - contig621end:23517-23536 MsG0080049033.01.T01:CDS 30.0%
TCAATTAAACACTGAACTTC+AGG - contig621end:22787-22806 MsG0080049033.01.T01:intron 30.0%
TGGATATATAAGATGAGGTT+TGG - contig621end:23606-23625 MsG0080049033.01.T01:CDS 30.0%
TTTGGTTAAACAATTGCAAC+AGG - contig621end:23368-23387 MsG0080049033.01.T01:intron 30.0%
! ACAAAGCAACATCTTTAGAA+GGG - contig621end:22873-22892 MsG0080049033.01.T01:intron 30.0%
! CCAAACGTTAAATTCACTTT+TGG - contig621end:24185-24204 MsG0080049033.01.T01:CDS 30.0%
! CCAGTTAACTTTGCAATTTT+AGG - contig621end:24538-24557 MsG0080049033.01.T01:intron 30.0%
! TCTTCTTCCTTTTCTTTTAG+GGG - contig621end:23841-23860 MsG0080049033.01.T01:intron 30.0%
! TTGATGATTAAAGACATACC+TGG - contig621end:24737-24756 MsG0080049033.01.T01:five_prime_UTR 30.0%
! TTTGCTTTGTCTTTTATCCT+GGG - contig621end:23410-23429 MsG0080049033.01.T01:intron 30.0%
!! GTTAAATTCACTTTTGGACT+TGG - contig621end:24191-24210 MsG0080049033.01.T01:CDS 30.0%
!! TAAAGATGTTGCTTTGTCTT+GGG + contig621end:22870-22889 None:intergenic 30.0%
!! TTACCAAAGTATTGTTGTGA+AGG - contig621end:23956-23975 MsG0080049033.01.T01:CDS 30.0%
ATCTGAGCTATCTAAACTAC+AGG - contig621end:24664-24683 MsG0080049033.01.T01:CDS 35.0%
CATGGACCTAGAGATAAAAT+AGG - contig621end:24058-24077 MsG0080049033.01.T01:CDS 35.0%
CATTGCAAACCTCTTTGAAT+TGG + contig621end:23168-23187 None:intergenic 35.0%
CTTTGAATTGGTCCTTAAGA+TGG + contig621end:23156-23175 None:intergenic 35.0%
GAAGGAGCTTGATATCAATT+GGG - contig621end:23974-23993 MsG0080049033.01.T01:CDS 35.0%
GACAAAGCAACATCTTTAGA+AGG - contig621end:22872-22891 MsG0080049033.01.T01:intron 35.0%
GATGGTGTTCGGTATAAATT+GGG - contig621end:24119-24138 MsG0080049033.01.T01:CDS 35.0%
GTTCATGCATAATATGCGTA+GGG - contig621end:24289-24308 MsG0080049033.01.T01:intron 35.0%
GTTTATGATGAGAATGCATC+TGG + contig621end:24484-24503 None:intergenic 35.0%
TCTTAAGGACCAATTCAAAG+AGG - contig621end:23156-23175 MsG0080049033.01.T01:intron 35.0%
TGAAGGAGCTTGATATCAAT+TGG - contig621end:23973-23992 MsG0080049033.01.T01:CDS 35.0%
TGAATAAAAGTTGCGCCTTT+GGG - contig621end:22139-22158 MsG0080049033.01.T01:CDS 35.0%
TGACATGATCATAAATGGAG+AGG - contig621end:23522-23541 MsG0080049033.01.T01:CDS 35.0%
TGGTCTGGATATATAAGATG+AGG - contig621end:23601-23620 MsG0080049033.01.T01:CDS 35.0%
TGTAAAGCTGCAAATTTAGC+AGG + contig621end:24785-24804 None:intergenic 35.0%
TGTTCATGCATAATATGCGT+AGG - contig621end:24288-24307 MsG0080049033.01.T01:intron 35.0%
TTGAATAAAAGTTGCGCCTT+TGG - contig621end:22138-22157 MsG0080049033.01.T01:CDS 35.0%
! AAAATTAACTTGGACGGTGA+GGG + contig621end:24379-24398 None:intergenic 35.0%
! AAAGAGAAGGGTACAAACAA+GGG + contig621end:23885-23904 None:intergenic 35.0%
! AAGGCGCAACTTTTATTCAA+CGG + contig621end:22138-22157 None:intergenic 35.0%
! CTATTTTATCTCTAGGTCCA+TGG + contig621end:24060-24079 None:intergenic 35.0%
! CTTGTTGTAGCTTGAACAAA+TGG - contig621end:23075-23094 MsG0080049033.01.T01:intron 35.0%
! GTTTGCTTTGTCTTTTATCC+TGG - contig621end:23409-23428 MsG0080049033.01.T01:intron 35.0%
! TATATGTCGTACCGAGTTTT+TGG + contig621end:22560-22579 None:intergenic 35.0%
! TCCTGCAAAATTAACTTGGA+CGG + contig621end:24385-24404 None:intergenic 35.0%
! TGATGGTGTTCGGTATAAAT+TGG - contig621end:24118-24137 MsG0080049033.01.T01:CDS 35.0%
! TTCAGGCCTATTTTATCTCT+AGG + contig621end:24067-24086 None:intergenic 35.0%
!! CTAAAGATGTTGCTTTGTCT+TGG + contig621end:22871-22890 None:intergenic 35.0%
!!! TCTCTTTTTTTCCCTCTCTA+TGG + contig621end:22921-22940 None:intergenic 35.0%
AAGCACACCCCTAAAAGAAA+AGG + contig621end:23851-23870 None:intergenic 40.0%
AGTACAAACCAAAAAACCCG+AGG - contig621end:24242-24261 MsG0080049033.01.T01:CDS 40.0%
ATATAAGATGAGGTTTGGGC+AGG - contig621end:23611-23630 MsG0080049033.01.T01:intron 40.0%
ATATGTGCATAGCATGTGCT+CGG + contig621end:22335-22354 None:intergenic 40.0%
ATCTTTCAGAGACCATAGAG+AGG - contig621end:22906-22925 MsG0080049033.01.T01:intron 40.0%
CAAAACACCAGCAAAGAGAA+GGG + contig621end:23897-23916 None:intergenic 40.0%
GAATAAAAGTTGCGCCTTTG+GGG - contig621end:22140-22159 MsG0080049033.01.T01:CDS 40.0%
GCTCCTTCACAACAATACTT+TGG + contig621end:23962-23981 None:intergenic 40.0%
TATCCATAGCCATGTTGTGA+TGG - contig621end:24101-24120 MsG0080049033.01.T01:CDS 40.0%
TCAAAACACCAGCAAAGAGA+AGG + contig621end:23898-23917 None:intergenic 40.0%
TCTTTCAGAGACCATAGAGA+GGG - contig621end:22907-22926 MsG0080049033.01.T01:intron 40.0%
TTATTATTCGAACGCAACGC+AGG - contig621end:22446-22465 MsG0080049033.01.T01:intron 40.0%
! AAATTAACTTGGACGGTGAG+GGG + contig621end:24378-24397 None:intergenic 40.0%
! AAGAGAAGGGTACAAACAAG+GGG + contig621end:23884-23903 None:intergenic 40.0%
! ACATCTTTAGAAGGGAAGCT+AGG - contig621end:22881-22900 MsG0080049033.01.T01:intron 40.0%
! ACCGTCCAAGTTAATTTTGC+AGG - contig621end:24381-24400 MsG0080049033.01.T01:CDS 40.0%
! CAAAATTAACTTGGACGGTG+AGG + contig621end:24380-24399 None:intergenic 40.0%
! CAAAGAGAAGGGTACAAACA+AGG + contig621end:23886-23905 None:intergenic 40.0%
! CGAGAACATTGAGCTTTTGA+AGG - contig621end:24601-24620 MsG0080049033.01.T01:CDS 40.0%
! TCTCTATGGTCTCTGAAAGA+TGG + contig621end:22907-22926 None:intergenic 40.0%
! TCTTTTCAGCTTCAGTCTTC+TGG + contig621end:23299-23318 None:intergenic 40.0%
! TGCGAAGGTTTGTCTCATTT+TGG + contig621end:22820-22839 None:intergenic 40.0%
! TTCTACAGGGAGTGATGTTT+TGG - contig621end:23350-23369 MsG0080049033.01.T01:intron 40.0%
!! GCTTGTTATCTATGAGGATC+CGG - contig621end:22288-22307 MsG0080049033.01.T01:CDS 40.0%
!!! ATTGGGCTGGTCTTTTTACT+TGG - contig621end:23991-24010 MsG0080049033.01.T01:CDS 40.0%
!!! TGGTATTTCCTCGGGTTTTT+TGG + contig621end:24253-24272 None:intergenic 40.0%
ACACCATCACAACATGGCTA+TGG + contig621end:24107-24126 None:intergenic 45.0%
ACTCCAGGATCAACTCTTGA+AGG - contig621end:22727-22746 MsG0080049033.01.T01:intron 45.0%
AGACCTGCTTAAAGAGCAAC+TGG - contig621end:23129-23148 MsG0080049033.01.T01:intron 45.0%
AGAGCAACTGGACCATCTTA+AGG - contig621end:23141-23160 MsG0080049033.01.T01:intron 45.0%
ATGACATGACAGACACTGAG+AGG - contig621end:22636-22655 MsG0080049033.01.T01:intron 45.0%
CAAGAACTCGCTGCTTCTAA+AGG - contig621end:22692-22711 MsG0080049033.01.T01:intron 45.0%
CACATATCAGTGTCGCCAAA+TGG - contig621end:22348-22367 MsG0080049033.01.T01:intron 45.0%
CACTGAGAGGAAGAAATCTG+TGG - contig621end:22649-22668 MsG0080049033.01.T01:intron 45.0%
CCCAGGAAGTTTCTTCTACA+GGG - contig621end:23337-23356 MsG0080049033.01.T01:intron 45.0%
CCCTGTAGAAGAAACTTCCT+GGG + contig621end:23340-23359 None:intergenic 45.0%
CTGGTGCAAACACTGGAAAT+AGG + contig621end:23280-23299 None:intergenic 45.0%
GAGCTTGATATCAATTGGGC+TGG - contig621end:23978-23997 MsG0080049033.01.T01:CDS 45.0%
GTTTGTACCCTTCTCTTTGC+TGG - contig621end:23887-23906 MsG0080049033.01.T01:intron 45.0%
TACCGAACACCATCACAACA+TGG + contig621end:24113-24132 None:intergenic 45.0%
TATCAGTGTCGCCAAATGGT+TGG - contig621end:22352-22371 MsG0080049033.01.T01:intron 45.0%
TCCCAGGAAGTTTCTTCTAC+AGG - contig621end:23336-23355 MsG0080049033.01.T01:intron 45.0%
TCCCTGTAGAAGAAACTTCC+TGG + contig621end:23341-23360 None:intergenic 45.0%
TGTTATCTATGAGGATCCGG+CGG - contig621end:22291-22310 MsG0080049033.01.T01:CDS 45.0%
TTGAAATCCCCAAACCCCAA+AGG + contig621end:22157-22176 None:intergenic 45.0%
TTTGAAGGAGAAACTGCTGG+AGG - contig621end:24616-24635 MsG0080049033.01.T01:CDS 45.0%
! AGCCATGTTGTGATGGTGTT+CGG - contig621end:24108-24127 MsG0080049033.01.T01:CDS 45.0%
! TGCTTCTAAAGGCCGTGAAA+AGG - contig621end:22703-22722 MsG0080049033.01.T01:intron 45.0%
!! AGATGTTGCTTTGTCTTGGG+CGG + contig621end:22867-22886 None:intergenic 45.0%
!! CGCACAGCTTGTTATCTATG+AGG - contig621end:22282-22301 MsG0080049033.01.T01:CDS 45.0%
!! GCTTTTGAAGGAGAAACTGC+TGG - contig621end:24613-24632 MsG0080049033.01.T01:CDS 45.0%
!!! GCTAGCAACTTTTTCATCCC+AGG - contig621end:23320-23339 MsG0080049033.01.T01:intron 45.0%
AAGCAGGTCTGCTTCTCTCT+CGG + contig621end:23119-23138 None:intergenic 50.0%
ACCTGGCATCTCATGTTTCG+AGG - contig621end:24754-24773 MsG0080049033.01.T01:five_prime_UTR 50.0%
CAGTCTTCTGGTGCAAACAC+TGG + contig621end:23287-23306 None:intergenic 50.0%
CCAGGATCAACTCTTGAAGG+AGG - contig621end:22730-22749 MsG0080049033.01.T01:intron 50.0%
GGTCCAGTTGCTCTTTAAGC+AGG + contig621end:23135-23154 None:intergenic 50.0%
TCCTCGAAACATGAGATGCC+AGG + contig621end:24758-24777 None:intergenic 50.0%
! CCTCCTTCAAGAGTTGATCC+TGG + contig621end:22733-22752 None:intergenic 50.0%
! TGATCGCGCTGGTATTTCCT+CGG + contig621end:24262-24281 None:intergenic 50.0%
!! AAAGTTGCGCCTTTGGGGTT+TGG - contig621end:22145-22164 MsG0080049033.01.T01:CDS 50.0%
!! AAGTTGCGCCTTTGGGGTTT+GGG - contig621end:22146-22165 MsG0080049033.01.T01:CDS 50.0%
!! GATCCTGGAGTGCCTTTTCA+CGG + contig621end:22718-22737 None:intergenic 50.0%
AAGAGGCCACGTTCCGATGA+CGG - contig621end:22250-22269 MsG0080049033.01.T01:CDS 55.0%
AGATGAGGTTTGGGCAGGCT+TGG - contig621end:23616-23635 MsG0080049033.01.T01:intron 55.0%
AGGAAAAGAGCTGCCGGGAA+AGG - contig621end:24636-24655 MsG0080049033.01.T01:CDS 55.0%
GAACAGAAGCTTGATCGCGC+TGG + contig621end:24273-24292 None:intergenic 55.0%
GCTCAGATTGTGCCCTTTCC+CGG + contig621end:24652-24671 None:intergenic 55.0%
GGAAAAGAGCTGCCGGGAAA+GGG - contig621end:24637-24656 MsG0080049033.01.T01:CDS 55.0%
GTGCATAGCATGTGCTCGGA+GGG + contig621end:22331-22350 None:intergenic 55.0%
TCATCGGAACGTGGCCTCTT+CGG + contig621end:22250-22269 None:intergenic 55.0%
TGCTGGAGGAAAAGAGCTGC+CGG - contig621end:24630-24649 MsG0080049033.01.T01:CDS 55.0%
TGCTTTGTCTTGGGCGGAAG+CGG + contig621end:22861-22880 None:intergenic 55.0%
TGTGCATAGCATGTGCTCGG+AGG + contig621end:22332-22351 None:intergenic 55.0%
! GAGACTCGGCTTGTATGCGA+AGG + contig621end:22835-22854 None:intergenic 55.0%
! GATCGCGCTGGTATTTCCTC+GGG + contig621end:24261-24280 None:intergenic 55.0%
!! AGGGGAGGGGTGTCAATACA+TGG - contig621end:24433-24452 MsG0080049033.01.T01:intron 55.0%
!! AGTTGCGCCTTTGGGGTTTG+GGG - contig621end:22147-22166 MsG0080049033.01.T01:CDS 55.0%
!! ATCAAGAGGCACTGAGACCC+AGG + contig621end:23430-23449 None:intergenic 55.0%
GCTGGAGGAAAAGAGCTGCC+GGG - contig621end:24631-24650 MsG0080049033.01.T01:CDS 60.0%
!! AGGCCGTGAAAAGGCACTCC+AGG - contig621end:22712-22731 MsG0080049033.01.T01:intron 60.0%
!! TCTTCGGTGGCGGAGGAGTA+CGG + contig621end:22234-22253 None:intergenic 60.0%
CTATGAGGATCCGGCGGCAG+CGG - contig621end:22297-22316 MsG0080049033.01.T01:CDS 65.0%
GAACGTGGCCTCTTCGGTGG+CGG + contig621end:22244-22263 None:intergenic 65.0%
GGCGGAAGCGGCATGAGACT+CGG + contig621end:22849-22868 None:intergenic 65.0%
GTACTCCTCCGCCACCGAAG+AGG - contig621end:22233-22252 MsG0080049033.01.T01:CDS 65.0%
TCGGAACGTGGCCTCTTCGG+TGG + contig621end:22247-22266 None:intergenic 65.0%
! CCACGTTCCGATGACGGCGA+CGG - contig621end:22256-22275 MsG0080049033.01.T01:CDS 65.0%
CCGTCGCCGTCATCGGAACG+TGG + contig621end:22259-22278 None:intergenic 70.0%
TGCGTCGCCGTCGCCGTCAT+CGG + contig621end:22266-22285 None:intergenic 70.0%
GGTTCCTCCACCGCTGCCGC+CGG + contig621end:22310-22329 None:intergenic 75.0%
TGAGGATCCGGCGGCAGCGG+TGG - contig621end:22300-22319 MsG0080049033.01.T01:CDS 75.0%
! CGTGGCCTCTTCGGTGGCGG+AGG + contig621end:22241-22260 None:intergenic 75.0%
! GGATCCGGCGGCAGCGGTGG+AGG - contig621end:22303-22322 MsG0080049033.01.T01:CDS 80.0%
Chromosome Type Strat End Strand Name
contig621end gene 22092 24823 22092 ID=MsG0080049033.01;Name=MsG0080049033.01
contig621end mRNA 22092 24823 22092 ID=MsG0080049033.01.T01;Parent=MsG0080049033.01;Name=MsG0080049033.01.T01;_AED=0.49;_eAED=0.50;_QI=131|1|0.83|1|1|1|6|0|359
contig621end exon 22092 22355 22092 ID=MsG0080049033.01.T01:exon:1998;Parent=MsG0080049033.01.T01
contig621end exon 23511 23627 23511 ID=MsG0080049033.01.T01:exon:1997;Parent=MsG0080049033.01.T01
contig621end exon 23738 23830 23738 ID=MsG0080049033.01.T01:exon:1996;Parent=MsG0080049033.01.T01
contig621end exon 23909 24267 23909 ID=MsG0080049033.01.T01:exon:1995;Parent=MsG0080049033.01.T01
contig621end exon 24348 24447 24348 ID=MsG0080049033.01.T01:exon:1994;Parent=MsG0080049033.01.T01
contig621end exon 24546 24823 24546 ID=MsG0080049033.01.T01:exon:1993;Parent=MsG0080049033.01.T01
contig621end five_prime_UTR 24693 24823 24693 ID=MsG0080049033.01.T01:five_prime_utr;Parent=MsG0080049033.01.T01
contig621end CDS 24546 24692 24546 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
contig621end CDS 24348 24447 24348 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
contig621end CDS 23909 24267 23909 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
contig621end CDS 23738 23830 23738 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
contig621end CDS 23511 23627 23511 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
contig621end CDS 22092 22355 22092 ID=MsG0080049033.01.T01:cds;Parent=MsG0080049033.01.T01
Gene Sequence

>MsG0080049033.01.T01

ATGATACTCCGTACTCCTCCGCCACCGAAGAGGCCACGTTCCGATGACGGCGACGGCGACGCACAGCTTGTTATCTATGAGGATCCGGCGGCAGCGGTGGAGGAACCCTCCGAGCACATGCTATGCACATATCAGTGTCGCCAAATGGTTAAATCTGATTTTCTAGATGCCTTAAGTAAAGCAGAAAATCAAGTTCGTGATTATCAATCTCAATTCCAAACACTCAATCATAATTTCCAAAAACTCGACACTGAGAGGAAGAAATCTGTGGAGCAATTGTTATACACTGAACAAGAACTCGCTGCTTCTAAAGGCCGTGAAAAGGCACTCCAGGATCAACTCTTGAAGGAGGTTACTGATTCTCAAGATAGACTTAGAAAATATATTCAATTAAACACTGAACTTCAGGCTAAGCTCCAAAATGAGACAAACCTTCGCATACAAGCCGAGTCTCATGCCGCTTCCGCCCAAGACAAAGCAACATCTTTAGAAGGGAAGCTAGGCCATCTTTCAGAGACCATAGAGAGGGAAAAAAAGAGACTTCACGACGATCATTCACAACTTAAAAAGGACTCCAACTTTTCTATTTCTAGAATCACCGCTAATCTTGAACAAATGGAATGCAGAGCTAATAATGCCGAGAGAGAAGCAGACCTGCTTAAAGAGCAACTGGACCATCTTAAGGACCAATTCAAAGAGTGTTTGCACCAGAAGACTGAAGCTGAAAAGAAGCTAGCAACTTTTTCATCCCAGGAAGTTTCTTCTACAGGGAGTGATGTTTTGGTTAAACAATTGCAACAGGAACTTCAACATTATGAATCTGAAGTAAGGGAAGCACGAAAACTAAGATCAAATCACGAGAACATTGAGCTTTTGAAGGAGAAACTGCTGGAGGAAAAGAGCTGCCGGGAAAGGGCACAATCTGAGCTATCTAAACTACAGGATGTCCAGTCAAATATGAAAAAGTTAGAGGATCAAATATCATCATGGAGATTGATGATTAAAGACATACCTGGCATCTCATGTTTCGAGGACATGCCTGCTAAATTTGCAGCTTTACAGAAGTACGTTAGTAGCTAA
Protein sequence

>MsG0080049033.01.T01

MILRTPPPPKRPRSDDGDGDAQLVIYEDPAAAVEEPSEHMLCTYQCRQMVKSDFLDALSKAENQVRDYQSQFQTLNHNFQKLDTERKKSVEQLLYTEQELAASKGREKALQDQLLKEVTDSQDRLRKYIQLNTELQAKLQNETNLRIQAESHAASAQDKATSLEGKLGHLSETIEREKKRLHDDHSQLKKDSNFSISRITANLEQMECRANNAEREADLLKEQLDHLKDQFKECLHQKTEAEKKLATFSSQEVSSTGSDVLVKQLQQELQHYESEVREARKLRSNHENIELLKEKLLEEKSCRERAQSELSKLQDVQSNMKKLEDQISSWRLMIKDIPGISCFEDMPAKFAALQKYVSS*