Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027361.01.T01 | XP_003614571.1 | 99.225 | 129 | 1 | 0 | 1 | 129 | 1 | 129 | 3.96E-83 | 249 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048113.01 | MsG0580027361.01 | 0.801998 | 6.909772e-49 | 3.346990e-46 |
| MsG0080048152.01 | MsG0580027361.01 | 0.803173 | 3.955943e-49 | 1.974479e-46 |
| MsG0080048383.01 | MsG0580027361.01 | 0.810617 | 1.054611e-50 | 6.387441e-48 |
| MsG0080048404.01 | MsG0580027361.01 | 0.827169 | 1.815248e-54 | 1.738726e-51 |
| MsG0080048462.01 | MsG0580027361.01 | 0.809979 | 1.447500e-50 | 8.621343e-48 |
| MsG0080048618.01 | MsG0580027361.01 | 0.839068 | 1.976599e-57 | 2.698708e-54 |
| MsG0080048774.01 | MsG0580027361.01 | 0.833606 | 4.840864e-56 | 5.602928e-53 |
| MsG0080048784.01 | MsG0580027361.01 | 0.857251 | 1.854234e-62 | 4.547504e-59 |
| MsG0080048817.01 | MsG0580027361.01 | 0.819534 | 1.104022e-52 | 8.519011e-50 |
| MsG0080048856.01 | MsG0580027361.01 | 0.844778 | 6.121658e-59 | 9.989922e-56 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580027361.01.T01 | MTR_5g055650 | 99.225 | 129 | 1 | 0 | 1 | 129 | 1 | 129 | 4.79e-87 | 249 |
| MsG0580027361.01.T01 | MTR_3g462870 | 84.211 | 38 | 6 | 0 | 92 | 129 | 92 | 129 | 7.21e-13 | 61.2 |
Find 37 sgRNAs with CRISPR-Local
Find 410 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATCTTGCTTAACAAGTAC+AGG | 0.520028 | 5:-59109108 | None:intergenic |
| AACTAGAGTGGTTTCTGTCT+TGG | 0.406531 | 5:-59113943 | None:intergenic |
| AAGAAGTACACTATTCAACT+TGG | 0.603063 | 5:+59108600 | MsG0580027361.01.T01:CDS |
| AAGATGCAATCTCTTCAGGC+TGG | 0.480196 | 5:+59113782 | MsG0580027361.01.T01:CDS |
| AATCGAATTTGTGAAAGCAA+AGG | 0.600362 | 5:+59108347 | MsG0580027361.01.T01:five_prime_UTR |
| AATCTCTTCAGGCTGGGAAA+GGG | 0.515494 | 5:+59113789 | MsG0580027361.01.T01:CDS |
| ACAAAAGATGCAATCTCTTC+AGG | 0.356299 | 5:+59113778 | MsG0580027361.01.T01:CDS |
| ACTCATTCACCACCTATGAA+CGG | 0.679282 | 5:-59113855 | None:intergenic |
| ACTGTAACTTGACCGTTCAT+AGG | 0.485267 | 5:+59113843 | MsG0580027361.01.T01:three_prime_UTR |
| AGATGCAATCTCTTCAGGCT+GGG | 0.569375 | 5:+59113783 | MsG0580027361.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAATCTTGCTTAACAAGTAC+AGG | - | Chr5:59109111-59109130 | None:intergenic | 30.0% | |
| AACCACTTGGAATGTTTATT+AGG | + | Chr5:59109305-59109324 | MsG0580027361.01.T01:intron | 30.0% | |
| AATCGAATTTGTGAAAGCAA+AGG | + | Chr5:59108347-59108366 | MsG0580027361.01.T01:five_prime_UTR | 30.0% | |
| AATGCACAATAACTGTTCTA+AGG | - | Chr5:59112316-59112335 | None:intergenic | 30.0% | |
| ACAAAGAAACAAAGATCAAG+AGG | - | Chr5:59111667-59111686 | None:intergenic | 30.0% | |
| ACCACTTGGAATGTTTATTA+GGG | + | Chr5:59109306-59109325 | MsG0580027361.01.T01:intron | 30.0% | |
| AGAGGAGAACTCTAATATAA+GGG | - | Chr5:59111106-59111125 | None:intergenic | 30.0% | |
| AGCAGACGATTAAACTTTAT+CGG | + | Chr5:59110095-59110114 | MsG0580027361.01.T01:intron | 30.0% | |
| AGGTATCTTGAAGAATCATT+TGG | - | Chr5:59111065-59111084 | None:intergenic | 30.0% | |
| ATATTTATCTCAGGATGTCT+TGG | + | Chr5:59111561-59111580 | MsG0580027361.01.T01:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 59108340 | 59114095 | 59108340 | ID=MsG0580027361.01;Name=MsG0580027361.01 |
| Chr5 | mRNA | 59108340 | 59114095 | 59108340 | ID=MsG0580027361.01.T01;Parent=MsG0580027361.01;Name=MsG0580027361.01.T01;_AED=0.44;_eAED=0.44;_QI=54|1|1|1|1|1|5|275|129 |
| Chr5 | exon | 59108340 | 59108469 | 59108340 | ID=MsG0580027361.01.T01:exon:13279;Parent=MsG0580027361.01.T01 |
| Chr5 | exon | 59108574 | 59108644 | 59108574 | ID=MsG0580027361.01.T01:exon:13280;Parent=MsG0580027361.01.T01 |
| Chr5 | exon | 59109060 | 59109184 | 59109060 | ID=MsG0580027361.01.T01:exon:13281;Parent=MsG0580027361.01.T01 |
| Chr5 | exon | 59112782 | 59112842 | 59112782 | ID=MsG0580027361.01.T01:exon:13282;Parent=MsG0580027361.01.T01 |
| Chr5 | exon | 59113764 | 59114095 | 59113764 | ID=MsG0580027361.01.T01:exon:13283;Parent=MsG0580027361.01.T01 |
| Chr5 | five_prime_UTR | 59108340 | 59108393 | 59108340 | ID=MsG0580027361.01.T01:five_prime_utr;Parent=MsG0580027361.01.T01 |
| Chr5 | CDS | 59108394 | 59108469 | 59108394 | ID=MsG0580027361.01.T01:cds;Parent=MsG0580027361.01.T01 |
| Chr5 | CDS | 59108574 | 59108644 | 59108574 | ID=MsG0580027361.01.T01:cds;Parent=MsG0580027361.01.T01 |
| Chr5 | CDS | 59109060 | 59109184 | 59109060 | ID=MsG0580027361.01.T01:cds;Parent=MsG0580027361.01.T01 |
| Chr5 | CDS | 59112782 | 59112842 | 59112782 | ID=MsG0580027361.01.T01:cds;Parent=MsG0580027361.01.T01 |
| Chr5 | CDS | 59113764 | 59113820 | 59113764 | ID=MsG0580027361.01.T01:cds;Parent=MsG0580027361.01.T01 |
| Chr5 | three_prime_UTR | 59113821 | 59114095 | 59113821 | ID=MsG0580027361.01.T01:three_prime_utr;Parent=MsG0580027361.01.T01 |
| Gene Sequence |
>MsG0580027361.01.T01 ATGAGTTCATCCAACATTAGAGATCTTCAACGCGAATTGGAGAACAAAGCTAACGATCTCAACAAGCTTCAAAAGGATATTGCGAAGAATCATCAAGTGAGAAAGAAGTACACTATTCAACTTGGCGAAAATGAGCTCGTTCTCAAGGAGTTGGATTTATTGCAAGAAGACGCAAATGTTTACAAGTTGATTGGACCTGTACTTGTTAAGCAAGATTTGGCTGAGGCCAATGCAAATGTTCGCAAGAGAATTGAATACATTTCAGCTGAACTGAAGCGTCTTGATGCCACGGTTCAAGATTTGGAAGAGAAGCAGAATAGCAAGAAAGAAACGATAATGAAATTGCAACAAAAGATGCAATCTCTTCAGGCTGGGAAAGGGAAGGCGTAG |
| Protein sequence |
>MsG0580027361.01.T01 MSSSNIRDLQRELENKANDLNKLQKDIAKNHQVRKKYTIQLGENELVLKELDLLQEDANVYKLIGPVLVKQDLAEANANVRKRIEYISAELKRLDATVQDLEEKQNSKKETIMKLQQKMQSLQAGKGKA* |
