AlfalfaGEDB | MsG0480022125.01

M. sativa cultivar ZhongmuNo.1 / MsG0480022125.01

KEGG: -
GO: -
PFAM: PF14009

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022125.01.T01	XP_013456859.1	97.906	191	4	0	1	191	1	191	6.59E-131	377

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022125.01.T01	Q6NLC8	46.067	178	78	5	1	164	1	174	5.80E-41	141

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022125.01.T01	J7GXG2	97.906	191	4	0	1	191	1	191	3.15e-131	377

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0480022125.01	0.820930	5.287783e-53	4.241703e-50
MsG0080048036.01	MsG0480022125.01	0.826865	2.145122e-54	2.036457e-51
MsG0080048301.01	MsG0480022125.01	0.808257	3.384320e-50	1.926013e-47
MsG0080048302.01	MsG0480022125.01	0.845984	2.887826e-59	4.898727e-56
MsG0080048345.01	MsG0480022125.01	0.847075	1.454056e-59	2.554778e-56
MsG0080048347.01	MsG0480022125.01	0.826532	2.576248e-54	2.422470e-51
MsG0080048348.01	MsG0480022125.01	0.835258	1.863667e-56	2.266887e-53
MsG0080048349.01	MsG0480022125.01	0.818220	2.195797e-52	1.633724e-49
MsG0080048350.01	MsG0480022125.01	0.823190	1.582793e-53	1.353335e-50
MsG0080048352.01	MsG0480022125.01	0.834869	2.335813e-56	2.807887e-53

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PPI

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Gene1	Gene2	Type
MsG0380015315.01	MsG0480022125.01	PPI
MsG0380015315.01	MsG0480022125.01	PPI
MsG0380015315.01	MsG0480022125.01	PPI
MsG0480022125.01	MsG0580028988.01	PPI
MsG0480022125.01	MsG0380015315.01	PPI
MsG0480022125.01	MsG0380015315.01	PPI
MsG0480022125.01	MsG0380015315.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022125.01.T01	MTR_4g085100	97.906	191	4	0	1	191	1	191	7.98e-135	377
MsG0480022125.01.T01	MTR_1g030740	71.098	173	46	3	1	172	1	170	4.04e-76	228
MsG0480022125.01.T01	MTR_3g067730	51.445	173	72	5	1	164	1	170	6.67e-49	159
MsG0480022125.01.T01	MTR_5g079610	50.282	177	74	5	1	168	1	172	7.18e-49	159

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480022125.01.T01	AT2G01340	59.302	172	65	1	1	172	1	167	3.62e-61	190
MsG0480022125.01.T01	AT1G71015	51.497	167	69	3	1	163	1	159	8.79e-45	148
MsG0480022125.01.T01	AT1G71015	51.497	167	69	3	1	163	1	159	8.79e-45	148
MsG0480022125.01.T01	AT1G66480	46.067	178	78	5	1	164	1	174	5.91e-42	141
MsG0480022125.01.T01	AT5G37840	42.373	177	88	5	1	170	1	170	3.51e-36	126

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Find 52 sgRNAs with CRISPR-Local

Find 55 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTGGAGAAGTGCTTA+AGG	0.461047	4:+68566821	MsG0480022125.01.T01:CDS
AAACCAAGAAGAATTCGTTC+CGG	0.593057	4:+68566975	MsG0480022125.01.T01:CDS
AAAGTAAGGAGAATGGTGGT+GGG	0.541122	4:+68567111	MsG0480022125.01.T01:CDS
AACTCCGAGAACACCACGAC+AGG	0.518893	4:+68567292	MsG0480022125.01.T01:CDS
AACTTCTCCACCTCTGCCTT+AGG	0.479167	4:-68567155	None:intergenic
AAGTAAGGAGAATGGTGGTG+GGG	0.675900	4:+68567112	MsG0480022125.01.T01:CDS
AATCGAAAACAAACCTGTCG+TGG	0.610738	4:-68567305	None:intergenic
AATGATTATGCGTTTGTATA+AGG	0.263418	4:+68567214	MsG0480022125.01.T01:CDS
ACAAAGAGTTACAACCAAAG+AGG	0.621866	4:+68566919	MsG0480022125.01.T01:CDS
ACAAAGGTCATGAAAATAGA+TGG	0.431872	4:+68566771	MsG0480022125.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACAAAGGTCATGAAAATAGA+TGG	+	Chr4:68566771-68566790	MsG0480022125.01.T01:CDS	30.0%
ACAAGAAAATAAAGCCTCTT+TGG	-	Chr4:68566936-68566955	None:intergenic	30.0%
CTAAAAACACCAATAAAAGC+TGG	+	Chr4:68566807-68566826	MsG0480022125.01.T01:CDS	30.0%
GAATGATAATAGTGGTGAAT+TGG	+	Chr4:68567088-68567107	MsG0480022125.01.T01:CDS	30.0%
AAACCAAGAAGAATTCGTTC+CGG	+	Chr4:68566975-68566994	MsG0480022125.01.T01:CDS	35.0%
ACAAAGAGTTACAACCAAAG+AGG	+	Chr4:68566919-68566938	MsG0480022125.01.T01:CDS	35.0%
CAGTTAAGCATTATGGTGTT+AGG	+	Chr4:68566877-68566896	MsG0480022125.01.T01:CDS	35.0%
GAATCTAACAAAACAAGACC+TGG	-	Chr4:68566852-68566871	None:intergenic	35.0%
GAATTGGAAAGTAAGGAGAA+TGG	+	Chr4:68567104-68567123	MsG0480022125.01.T01:CDS	35.0%
GATGAGAGATTGTAAAGATG+AGG	+	Chr4:68567178-68567197	MsG0480022125.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	68566741	68567370	68566741	ID=MsG0480022125.01;Name=MsG0480022125.01
Chr4	mRNA	68566741	68567370	68566741	ID=MsG0480022125.01.T01;Parent=MsG0480022125.01;Name=MsG0480022125.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|209
Chr4	exon	68566741	68567370	68566741	ID=MsG0480022125.01.T01:exon:856;Parent=MsG0480022125.01.T01
Chr4	CDS	68566741	68567370	68566741	ID=MsG0480022125.01.T01:cds;Parent=MsG0480022125.01.T01

Gene Sequence

>MsG0480022125.01.T01

ATGGGGAACACTTTTGGTTCAAAAAAGACCACAAAGGTCATGAAAATAGATGGTGAAACAATGAAGCTAAAAACACCAATAAAAGCTGGAGAAGTGCTTAAGGATCATCCAGGTCTTGTTTTGTTAGATTCTGAAGCAGTTAAGCATTATGGTGTTAGGGCAAAGGCAATTGAAGCACACAAAGAGTTACAACCAAAGAGGCTTTATTTTCTTGTGGAGCTTCCAAAAGAAACCAAACCAAGAAGAATTCGTTCCGGAATTCAAATGAGTGCCAAAGATAGACTTGATAATTTGGTGCTTACTAGGAGATCTGCTTCTGATTTGTCAATTTTGAAACAAAGTAAAATGAATGATAATAGTGGTGAATTGGAAAGTAAGGAGAATGGTGGTGGGGTGAGGTTGAAAGTTAGACTTCCTAAGGCAGAGGTGGAGAAGTTGATGAGAGATTGTAAAGATGAGGCTGAGGCTGCAGAAATGATTATGCGTTTGTATAAGGCCAGTGGCAAAGGGGAAAAGGAGACTATGGTTAAGGAGAGTCCGGGGACTCCGAGAACTCCGAGAACACCACGACAGGTTTGTTTTCGATTAATTTTGCATGCTAATTTTTTTGTCTTAACCCTCAAATTCTAA

Protein sequence

>MsG0480022125.01.T01

MGNTFGSKKTTKVMKIDGETMKLKTPIKAGEVLKDHPGLVLLDSEAVKHYGVRAKAIEAHKELQPKRLYFLVELPKETKPRRIRSGIQMSAKDRLDNLVLTRRSASDLSILKQSKMNDNSGELESKENGGGVRLKVRLPKAEVEKLMRDCKDEAEAAEMIMRLYKASGKGEKETMVKESPGTPRTPRTPRQVCFRLILHANFFVLTLKF*