Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003767.01 | MsG0480022237.01 | 0.844423 | 7.630237e-59 | 1.231256e-55 |
| MsG0180004605.01 | MsG0480022237.01 | 0.821286 | 4.376072e-53 | 3.545105e-50 |
| MsG0180006034.01 | MsG0480022237.01 | 0.801483 | 8.814232e-49 | 4.214167e-46 |
| MsG0280011440.01 | MsG0480022237.01 | 0.815646 | 8.310748e-52 | 5.762897e-49 |
| MsG0380013794.01 | MsG0480022237.01 | 0.819113 | 1.377228e-52 | 1.050310e-49 |
| MsG0380014401.01 | MsG0480022237.01 | 0.803877 | 2.825894e-49 | 1.436272e-46 |
| MsG0480019689.01 | MsG0480022237.01 | 0.809119 | 2.214684e-50 | 1.289314e-47 |
| MsG0480021776.01 | MsG0480022237.01 | 0.813610 | 2.345537e-51 | 1.538962e-48 |
| MsG0480022237.01 | MsG0480022872.01 | 0.823287 | 1.502445e-53 | 1.288100e-50 |
| MsG0480022237.01 | MsG0480022939.01 | 0.803462 | 3.446704e-49 | 1.733100e-46 |
| MsG0480022237.01 | MsG0480023973.01 | 0.804246 | 2.368124e-49 | 1.214943e-46 |
| MsG0480022237.01 | MsG0680031333.01 | 0.831297 | 1.808059e-55 | 1.953765e-52 |
| MsG0480022237.01 | MsG0780041117.01 | 0.809217 | 2.109897e-50 | 1.231552e-47 |
| MsG0480022237.01 | MsG0780041470.01 | 0.845859 | 3.122485e-59 | 5.275383e-56 |
| MsG0480022237.01 | MsG0880045010.01 | 0.824289 | 8.747917e-54 | 7.714957e-51 |
| MsG0480022237.01 | MsG0880045548.01 | 0.848796 | 4.877938e-60 | 9.056404e-57 |
| MsG0380015066.01 | MsG0480022237.01 | 0.801686 | 8.009518e-49 | 3.848950e-46 |
| MsG0380016347.01 | MsG0480022237.01 | 0.841291 | 5.195155e-58 | 7.597805e-55 |
| MsG0380016671.01 | MsG0480022237.01 | -0.814306 | 1.647962e-51 | 1.101861e-48 |
| MsG0480018091.01 | MsG0480022237.01 | 0.859576 | 3.760413e-63 | 9.977605e-60 |
| MsG0280007193.01 | MsG0480022237.01 | 0.859683 | 3.491642e-63 | 9.299129e-60 |
| MsG0280008304.01 | MsG0480022237.01 | 0.808719 | 2.697785e-50 | 1.553766e-47 |
| MsG0280008307.01 | MsG0480022237.01 | 0.805063 | 1.599844e-49 | 8.382799e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 48 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATCGTGATCTTGACGTTT+AGG | 0.253452 | 4:+70019221 | MsG0480022237.01.T01:CDS |
| GGTTGGGGTGACGGAGAGGG+TGG | 0.297849 | 4:+70019487 | MsG0480022237.01.T01:CDS |
| CGGATTTAGAGAGGGTGGTT+GGG | 0.325848 | 4:+70019471 | MsG0480022237.01.T01:CDS |
| GATCGTGATCTTGACGTTTA+GGG | 0.353126 | 4:+70019222 | MsG0480022237.01.T01:CDS |
| TTGACGTTTAGGGTTGATTT+CGG | 0.367421 | 4:+70019232 | MsG0480022237.01.T01:CDS |
| TTTAGGGTTGATTTCGGATT+GGG | 0.376201 | 4:+70019238 | MsG0480022237.01.T01:CDS |
| GGGTGGTTGGGGTGACGGAG+AGG | 0.388755 | 4:+70019483 | MsG0480022237.01.T01:CDS |
| AGTCGAGATCGGAAGGGGAT+TGG | 0.409731 | 4:+70019417 | MsG0480022237.01.T01:CDS |
| AGAGAGGGTGGTTGGGGTGA+CGG | 0.411801 | 4:+70019478 | MsG0480022237.01.T01:CDS |
| AGTGCCGGAGCCGGATTGCC+AGG | 0.419413 | 4:+70019319 | MsG0480022237.01.T01:CDS |
| ACCGTCACCCTCCGGTGGTC+CGG | 0.422619 | 4:-70019443 | None:intergenic |
| CCGGATTTAGAGAGGGTGGT+TGG | 0.441118 | 4:+70019470 | MsG0480022237.01.T01:CDS |
| CCGGAGCCGGATTGCCAGGT+GGG | 0.445902 | 4:+70019323 | MsG0480022237.01.T01:CDS |
| GGTGGTTGGGGTGACGGAGA+GGG | 0.452623 | 4:+70019484 | MsG0480022237.01.T01:CDS |
| CCCACCTGGCAATCCGGCTC+CGG | 0.459111 | 4:-70019323 | None:intergenic |
| GTTTAGGGTTGATTTCGGAT+TGG | 0.462844 | 4:+70019237 | MsG0480022237.01.T01:CDS |
| GGATTTAGAGAGGGTGGTTG+GGG | 0.464704 | 4:+70019472 | MsG0480022237.01.T01:CDS |
| GTTGGGGTGACGGAGAGGGT+GGG | 0.468966 | 4:+70019488 | MsG0480022237.01.T01:CDS |
| GAGAGAGAGAGTGCCGGAGC+CGG | 0.482078 | 4:+70019310 | MsG0480022237.01.T01:CDS |
| ATCGGAAGGGGATTGGATAC+CGG | 0.490300 | 4:+70019424 | MsG0480022237.01.T01:CDS |
| GGAGCCGGATTGCCAGGTGG+GGG | 0.491459 | 4:+70019325 | MsG0480022237.01.T01:CDS |
| GAAGATACGGAAGGAGTAGT+TGG | 0.505181 | 4:+70019268 | MsG0480022237.01.T01:CDS |
| TGGATCGGAGGTTGATGAGA+GGG | 0.523548 | 4:+70019288 | MsG0480022237.01.T01:CDS |
| CGGAGCCGGATTGCCAGGTG+GGG | 0.525029 | 4:+70019324 | MsG0480022237.01.T01:CDS |
| CCAACCACCCTCTCTAAATC+CGG | 0.526565 | 4:-70019470 | None:intergenic |
| GGGATTGGATACCGGACCAC+CGG | 0.529993 | 4:+70019432 | MsG0480022237.01.T01:CDS |
| GATTGCGACGAAGTCGAGAT+CGG | 0.546347 | 4:+70019406 | MsG0480022237.01.T01:CDS |
| GCGACGAAGTCGAGATCGGA+AGG | 0.546449 | 4:+70019410 | MsG0480022237.01.T01:CDS |
| ATTGGGACCAGTGGAAGATA+CGG | 0.550432 | 4:+70019255 | MsG0480022237.01.T01:CDS |
| CCGGCGATACCGTCACCCTC+CGG | 0.550903 | 4:-70019451 | None:intergenic |
| GACGAAGTCGAGATCGGAAG+GGG | 0.564001 | 4:+70019412 | MsG0480022237.01.T01:CDS |
| TTGGATCGGAGGTTGATGAG+AGG | 0.574114 | 4:+70019287 | MsG0480022237.01.T01:CDS |
| GAGAGGGAGAGAGAGAGTGC+CGG | 0.575622 | 4:+70019304 | MsG0480022237.01.T01:CDS |
| GCCGGAGCCGGATTGCCAGG+TGG | 0.576026 | 4:+70019322 | MsG0480022237.01.T01:CDS |
| ACTCCTTCCGTATCTTCCAC+TGG | 0.587329 | 4:-70019262 | None:intergenic |
| CCGGAGGGTGACGGTATCGC+CGG | 0.593178 | 4:+70019451 | MsG0480022237.01.T01:CDS |
| TACGGAAGGAGTAGTTGGAT+CGG | 0.602673 | 4:+70019273 | MsG0480022237.01.T01:CDS |
| ATTGGATACCGGACCACCGG+AGG | 0.604498 | 4:+70019435 | MsG0480022237.01.T01:CDS |
| ACCGGACCACCGGAGGGTGA+CGG | 0.607343 | 4:+70019442 | MsG0480022237.01.T01:CDS |
| TTGGATACCGGACCACCGGA+GGG | 0.616726 | 4:+70019436 | MsG0480022237.01.T01:CDS |
| CGGTATCGCCGGATTTAGAG+AGG | 0.617002 | 4:+70019462 | MsG0480022237.01.T01:CDS |
| TGATTTCGGATTGGGACCAG+TGG | 0.617513 | 4:+70019246 | MsG0480022237.01.T01:CDS |
| CGACGAAGTCGAGATCGGAA+GGG | 0.638950 | 4:+70019411 | MsG0480022237.01.T01:CDS |
| ATCGCCGGATTTAGAGAGGG+TGG | 0.642421 | 4:+70019466 | MsG0480022237.01.T01:CDS |
| GGAAGGAGTAGTTGGATCGG+AGG | 0.659730 | 4:+70019276 | MsG0480022237.01.T01:CDS |
| GCGATACCGTCACCCTCCGG+TGG | 0.659789 | 4:-70019448 | None:intergenic |
| GGACCAGTGGAAGATACGGA+AGG | 0.668679 | 4:+70019259 | MsG0480022237.01.T01:CDS |
| GGTATCGCCGGATTTAGAGA+GGG | 0.705156 | 4:+70019463 | MsG0480022237.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTGACGTTTAGGGTTGATTT+CGG | + | Chr4:70019232-70019251 | MsG0480022237.01.T01:CDS | 35.0% |
| ! | TTTAGGGTTGATTTCGGATT+GGG | + | Chr4:70019238-70019257 | MsG0480022237.01.T01:CDS | 35.0% |
| GATCGTGATCTTGACGTTTA+GGG | + | Chr4:70019222-70019241 | MsG0480022237.01.T01:CDS | 40.0% | |
| TGATCGTGATCTTGACGTTT+AGG | + | Chr4:70019221-70019240 | MsG0480022237.01.T01:CDS | 40.0% | |
| ! | GTAGCCGATGTAGTTTTTGT+GGG | + | Chr4:70019364-70019383 | MsG0480022237.01.T01:CDS | 40.0% |
| ! | GTTTAGGGTTGATTTCGGAT+TGG | + | Chr4:70019237-70019256 | MsG0480022237.01.T01:CDS | 40.0% |
| ATTGGGACCAGTGGAAGATA+CGG | + | Chr4:70019255-70019274 | MsG0480022237.01.T01:CDS | 45.0% | |
| GAAGATACGGAAGGAGTAGT+TGG | + | Chr4:70019268-70019287 | MsG0480022237.01.T01:CDS | 45.0% | |
| TACGGAAGGAGTAGTTGGAT+CGG | + | Chr4:70019273-70019292 | MsG0480022237.01.T01:CDS | 45.0% | |
| TCCGCCCACAAAAACTACAT+CGG | - | Chr4:70019371-70019390 | None:intergenic | 45.0% | |
| ! | GGTAGCCGATGTAGTTTTTG+TGG | + | Chr4:70019363-70019382 | MsG0480022237.01.T01:CDS | 45.0% |
| ACTCCTTCCGTATCTTCCAC+TGG | - | Chr4:70019265-70019284 | None:intergenic | 50.0% | |
| ATCGGAAGGGGATTGGATAC+CGG | + | Chr4:70019424-70019443 | MsG0480022237.01.T01:CDS | 50.0% | |
| CCAACCACCCTCTCTAAATC+CGG | - | Chr4:70019473-70019492 | None:intergenic | 50.0% | |
| CGGATTTAGAGAGGGTGGTT+GGG | + | Chr4:70019471-70019490 | MsG0480022237.01.T01:CDS | 50.0% | |
| GATTGCGACGAAGTCGAGAT+CGG | + | Chr4:70019406-70019425 | MsG0480022237.01.T01:CDS | 50.0% | |
| GGATTTAGAGAGGGTGGTTG+GGG | + | Chr4:70019472-70019491 | MsG0480022237.01.T01:CDS | 50.0% | |
| GGTATCGCCGGATTTAGAGA+GGG | + | Chr4:70019463-70019482 | MsG0480022237.01.T01:CDS | 50.0% | |
| ! | GATGTAGTTTTTGTGGGCGG+AGG | + | Chr4:70019370-70019389 | MsG0480022237.01.T01:CDS | 50.0% |
| ! | GCCGATGTAGTTTTTGTGGG+CGG | + | Chr4:70019367-70019386 | MsG0480022237.01.T01:CDS | 50.0% |
| ! | TAGTTTTTGTGGGCGGAGGT+GGG | + | Chr4:70019374-70019393 | MsG0480022237.01.T01:CDS | 50.0% |
| ! | TGATTTCGGATTGGGACCAG+TGG | + | Chr4:70019246-70019265 | MsG0480022237.01.T01:CDS | 50.0% |
| !! | TGGATCGGAGGTTGATGAGA+GGG | + | Chr4:70019288-70019307 | MsG0480022237.01.T01:CDS | 50.0% |
| !! | TTGGATCGGAGGTTGATGAG+AGG | + | Chr4:70019287-70019306 | MsG0480022237.01.T01:CDS | 50.0% |
| AAATCCCCCACCTGGCAATC+CGG | - | Chr4:70019332-70019351 | None:intergenic | 55.0% | |
| AGTCGAGATCGGAAGGGGAT+TGG | + | Chr4:70019417-70019436 | MsG0480022237.01.T01:CDS | 55.0% | |
| ATCGCCGGATTTAGAGAGGG+TGG | + | Chr4:70019466-70019485 | MsG0480022237.01.T01:CDS | 55.0% | |
| CCGGATTTAGAGAGGGTGGT+TGG | + | Chr4:70019470-70019489 | MsG0480022237.01.T01:CDS | 55.0% | |
| CGACGAAGTCGAGATCGGAA+GGG | + | Chr4:70019411-70019430 | MsG0480022237.01.T01:CDS | 55.0% | |
| CGGTATCGCCGGATTTAGAG+AGG | + | Chr4:70019462-70019481 | MsG0480022237.01.T01:CDS | 55.0% | |
| GACGAAGTCGAGATCGGAAG+GGG | + | Chr4:70019412-70019431 | MsG0480022237.01.T01:CDS | 55.0% | |
| GGAAGGAGTAGTTGGATCGG+AGG | + | Chr4:70019276-70019295 | MsG0480022237.01.T01:CDS | 55.0% | |
| GGACCAGTGGAAGATACGGA+AGG | + | Chr4:70019259-70019278 | MsG0480022237.01.T01:CDS | 55.0% | |
| TGGGGGATTTGGAGAGGAAG+AGG | + | Chr4:70019342-70019361 | MsG0480022237.01.T01:CDS | 55.0% | |
| ! | GTAGTTTTTGTGGGCGGAGG+TGG | + | Chr4:70019373-70019392 | MsG0480022237.01.T01:CDS | 55.0% |
| AGAGAGGGTGGTTGGGGTGA+CGG | + | Chr4:70019478-70019497 | MsG0480022237.01.T01:CDS | 60.0% | |
| ATTGGATACCGGACCACCGG+AGG | + | Chr4:70019435-70019454 | MsG0480022237.01.T01:CDS | 60.0% | |
| GCGACGAAGTCGAGATCGGA+AGG | + | Chr4:70019410-70019429 | MsG0480022237.01.T01:CDS | 60.0% | |
| GGATTGCCAGGTGGGGGATT+TGG | + | Chr4:70019331-70019350 | MsG0480022237.01.T01:CDS | 60.0% | |
| GGGATTGGATACCGGACCAC+CGG | + | Chr4:70019432-70019451 | MsG0480022237.01.T01:CDS | 60.0% | |
| TCCTCTCCAAATCCCCCACC+TGG | - | Chr4:70019340-70019359 | None:intergenic | 60.0% | |
| TTGGATACCGGACCACCGGA+GGG | + | Chr4:70019436-70019455 | MsG0480022237.01.T01:CDS | 60.0% | |
| ! | GAGAGGGAGAGAGAGAGTGC+CGG | + | Chr4:70019304-70019323 | MsG0480022237.01.T01:CDS | 60.0% |
| ! | TTTTTGTGGGCGGAGGTGGG+TGG | + | Chr4:70019377-70019396 | MsG0480022237.01.T01:CDS | 60.0% |
| GCCAGGTGGGGGATTTGGAG+AGG | + | Chr4:70019336-70019355 | MsG0480022237.01.T01:CDS | 65.0% | |
| !! | GAGAGAGAGAGTGCCGGAGC+CGG | + | Chr4:70019310-70019329 | MsG0480022237.01.T01:CDS | 65.0% |
| !! | GGTGGTTGGGGTGACGGAGA+GGG | + | Chr4:70019484-70019503 | MsG0480022237.01.T01:CDS | 65.0% |
| !! | GTTGGGGTGACGGAGAGGGT+GGG | + | Chr4:70019488-70019507 | MsG0480022237.01.T01:CDS | 65.0% |
| ACCGGACCACCGGAGGGTGA+CGG | + | Chr4:70019442-70019461 | MsG0480022237.01.T01:CDS | 70.0% | |
| ACCGTCACCCTCCGGTGGTC+CGG | - | Chr4:70019446-70019465 | None:intergenic | 70.0% | |
| CCCACCTGGCAATCCGGCTC+CGG | - | Chr4:70019326-70019345 | None:intergenic | 70.0% | |
| CCGGAGCCGGATTGCCAGGT+GGG | + | Chr4:70019323-70019342 | MsG0480022237.01.T01:CDS | 70.0% | |
| CCGGCGATACCGTCACCCTC+CGG | - | Chr4:70019454-70019473 | None:intergenic | 70.0% | |
| CGGAGCCGGATTGCCAGGTG+GGG | + | Chr4:70019324-70019343 | MsG0480022237.01.T01:CDS | 70.0% | |
| GCGATACCGTCACCCTCCGG+TGG | - | Chr4:70019451-70019470 | None:intergenic | 70.0% | |
| GGAGCCGGATTGCCAGGTGG+GGG | + | Chr4:70019325-70019344 | MsG0480022237.01.T01:CDS | 70.0% | |
| ! | AGTGCCGGAGCCGGATTGCC+AGG | + | Chr4:70019319-70019338 | MsG0480022237.01.T01:CDS | 70.0% |
| ! | CCGGAGGGTGACGGTATCGC+CGG | + | Chr4:70019451-70019470 | MsG0480022237.01.T01:CDS | 70.0% |
| !! | GGGTGGTTGGGGTGACGGAG+AGG | + | Chr4:70019483-70019502 | MsG0480022237.01.T01:CDS | 70.0% |
| !! | GGTTGGGGTGACGGAGAGGG+TGG | + | Chr4:70019487-70019506 | MsG0480022237.01.T01:CDS | 70.0% |
| GCCGGAGCCGGATTGCCAGG+TGG | + | Chr4:70019322-70019341 | MsG0480022237.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr4 | gene | 70019220 | 70019516 | 70019220 | ID=MsG0480022237.01;Name=MsG0480022237.01 |
| Chr4 | mRNA | 70019220 | 70019516 | 70019220 | ID=MsG0480022237.01.T01;Parent=MsG0480022237.01;Name=MsG0480022237.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr4 | exon | 70019220 | 70019516 | 70019220 | ID=MsG0480022237.01.T01:exon:55460;Parent=MsG0480022237.01.T01 |
| Chr4 | CDS | 70019220 | 70019516 | 70019220 | ID=MsG0480022237.01.T01:cds;Parent=MsG0480022237.01.T01 |
| Gene Sequence |
| Protein sequence |
