Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014401.01.T01 | KEH33980.1 | 98.678 | 227 | 3 | 0 | 6 | 232 | 65 | 291 | 5.47E-164 | 471 |
| MsG0380014401.01.T01 | KEH33980.1 | 46.154 | 91 | 41 | 3 | 40 | 122 | 271 | 361 | 1.17E-11 | 75.9 |
| MsG0380014401.01.T02 | XP_003600260.2 | 97.945 | 146 | 3 | 0 | 1 | 146 | 1 | 146 | 1.09E-92 | 290 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014401.01.T01 | A0A072V751 | 98.678 | 227 | 3 | 0 | 6 | 232 | 65 | 291 | 2.61e-164 | 471 |
| MsG0380014401.01.T01 | G7J209 | 98.678 | 227 | 3 | 0 | 6 | 232 | 209 | 435 | 4.47e-163 | 473 |
| MsG0380014401.01.T02 | G7J210 | 97.945 | 146 | 3 | 0 | 1 | 146 | 1 | 146 | 5.18e-93 | 290 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080049069.01 | MsG0380014401.01 | -0.819604 | 1.064303e-52 | 8.227859e-50 |
| MsG0180000010.01 | MsG0380014401.01 | 0.814332 | 1.625933e-51 | 1.087874e-48 |
| MsG0180000240.01 | MsG0380014401.01 | 0.826415 | 2.746699e-54 | 2.574357e-51 |
| MsG0180000544.01 | MsG0380014401.01 | 0.831520 | 1.593498e-55 | 1.733480e-52 |
| MsG0180001248.01 | MsG0380014401.01 | 0.802935 | 4.429511e-49 | 2.197387e-46 |
| MsG0180003246.01 | MsG0380014401.01 | 0.831007 | 2.130007e-55 | 2.282106e-52 |
| MsG0180003767.01 | MsG0380014401.01 | 0.852500 | 4.432397e-61 | 9.280439e-58 |
| MsG0180005515.01 | MsG0380014401.01 | 0.817231 | 3.670282e-52 | 2.657712e-49 |
| MsG0180006034.01 | MsG0380014401.01 | 0.802192 | 6.303469e-49 | 3.068360e-46 |
| MsG0280006306.01 | MsG0380014401.01 | 0.803693 | 3.085600e-49 | 1.560792e-46 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014401.01.T01 | MTR_3g056100 | 98.678 | 227 | 3 | 0 | 6 | 232 | 65 | 291 | 6.62e-168 | 471 |
| MsG0380014401.01.T01 | MTR_3g056100 | 46.154 | 91 | 41 | 3 | 40 | 122 | 271 | 361 | 1.42e-15 | 75.9 |
| MsG0380014401.01.T01 | MTR_3g056100 | 98.678 | 227 | 3 | 0 | 6 | 232 | 209 | 435 | 1.13e-166 | 473 |
| MsG0380014401.01.T01 | MTR_3g056100 | 46.154 | 91 | 41 | 3 | 40 | 122 | 415 | 505 | 1.37e-15 | 76.3 |
| MsG0380014401.01.T01 | MTR_3g056100 | 93.833 | 227 | 1 | 1 | 6 | 232 | 65 | 278 | 4.51e-155 | 438 |
| MsG0380014401.01.T01 | MTR_3g056100 | 46.154 | 91 | 41 | 3 | 40 | 122 | 258 | 348 | 1.34e-15 | 75.9 |
| MsG0380014401.01.T01 | MTR_3g056100 | 93.833 | 227 | 1 | 1 | 6 | 232 | 209 | 422 | 8.20e-154 | 440 |
| MsG0380014401.01.T01 | MTR_3g056100 | 46.154 | 91 | 41 | 3 | 40 | 122 | 402 | 492 | 1.32e-15 | 76.3 |
| MsG0380014401.01.T01 | MTR_5g094430 | 67.100 | 231 | 41 | 4 | 7 | 232 | 50 | 250 | 8.82e-103 | 304 |
| MsG0380014401.01.T01 | MTR_5g094430 | 47.500 | 80 | 39 | 2 | 40 | 116 | 230 | 309 | 2.05e-15 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014401.01.T01 | AT4G26640 | 69.027 | 226 | 57 | 7 | 11 | 232 | 9 | 225 | 1.55e-100 | 298 |
| MsG0380014401.01.T01 | AT4G26640 | 58.333 | 60 | 24 | 1 | 40 | 98 | 205 | 264 | 4.86e-15 | 73.9 |
| MsG0380014401.01.T01 | AT4G26640 | 69.027 | 226 | 57 | 7 | 11 | 232 | 9 | 225 | 1.55e-100 | 298 |
| MsG0380014401.01.T01 | AT4G26640 | 58.333 | 60 | 24 | 1 | 40 | 98 | 205 | 264 | 4.86e-15 | 73.9 |
| MsG0380014401.01.T01 | AT4G26640 | 69.027 | 226 | 57 | 7 | 11 | 232 | 9 | 225 | 1.55e-100 | 298 |
| MsG0380014401.01.T01 | AT4G26640 | 58.333 | 60 | 24 | 1 | 40 | 98 | 205 | 264 | 4.86e-15 | 73.9 |
| MsG0380014401.01.T01 | AT4G26640 | 69.027 | 226 | 57 | 7 | 11 | 232 | 9 | 225 | 1.55e-100 | 298 |
| MsG0380014401.01.T01 | AT4G26640 | 58.333 | 60 | 24 | 1 | 40 | 98 | 205 | 264 | 4.86e-15 | 73.9 |
| MsG0380014401.01.T01 | AT4G26640 | 69.027 | 226 | 57 | 7 | 11 | 232 | 113 | 329 | 2.12e-99 | 298 |
| MsG0380014401.01.T01 | AT4G26640 | 58.333 | 60 | 24 | 1 | 40 | 98 | 309 | 368 | 6.02e-15 | 73.9 |
Find 307 sgRNAs with CRISPR-Local
Find 536 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCAATTCAATCTTTA+TGG | 0.080597 | 3:-54191205 | None:intergenic |
| AAAAGTTTGATCCTATGAAA+TGG | 0.424835 | 3:+54190056 | MsG0380014401.01.T01:three_prime_UTR |
| AAATAACTAATTACATCTAA+TGG | 0.333350 | 3:+54189948 | MsG0380014401.01.T01:three_prime_UTR |
| AAATACAGCGCAAATCCATA+TGG | 0.507523 | 3:+54192348 | MsG0380014401.01.T01:three_prime_UTR |
| AAATATTGTAGTTCTTCTCA+AGG | 0.588497 | 3:+54190907 | MsG0380014401.01.T01:three_prime_UTR |
| AAATGTACTTCTCACTTATT+TGG | 0.080263 | 3:+54189383 | MsG0380014401.01.T01:three_prime_UTR |
| AAATTTACTTCAATGCTCTC+AGG | 0.503252 | 3:+54190461 | MsG0380014401.01.T01:three_prime_UTR |
| AACATGGGAAGAATAATGAT+GGG | 0.637504 | 3:+54192433 | MsG0380014401.01.T01:three_prime_UTR |
| AACCCTCGGTTCACCGGTTT+AGG | 0.376451 | 3:+54189749 | MsG0380014401.01.T01:three_prime_UTR |
| AACTACAATATTTGGCTTAC+GGG | 0.340707 | 3:-54190897 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATCACAAAGTCAGTAG+AGG | - | Chr3:54189682-54189701 | None:intergenic | 30.0% | |
| AAATTTACTTCAATGCTCTC+AGG | + | Chr3:54190461-54190480 | MsG0380014401.01.T01:three_prime_UTR | 30.0% | |
| AACATGGGAAGAATAATGAT+GGG | + | Chr3:54192433-54192452 | MsG0380014401.01.T01:three_prime_UTR | 30.0% | |
| ACACTAAATATGAATGTGCA+TGG | + | Chr3:54191834-54191853 | MsG0380014401.01.T01:three_prime_UTR | 30.0% | |
| ACTATGAACAAACTTGAAAC+TGG | - | Chr3:54192297-54192316 | None:intergenic | 30.0% | |
| AGAGAAAGTCATATTCCAAT+AGG | - | Chr3:54186988-54187007 | None:intergenic | 30.0% | |
| AGTGTTGTCAGATCAAATAT+AGG | + | Chr3:54191746-54191765 | MsG0380014401.01.T01:three_prime_UTR | 30.0% | |
| AGTTGTACTTCAATTCACTT+AGG | - | Chr3:54186366-54186385 | None:intergenic | 30.0% | |
| AGTTTCTTTACTTCACAGTT+AGG | - | Chr3:54188597-54188616 | None:intergenic | 30.0% | |
| ATTGTATAGTCATCAATGTG+AGG | - | Chr3:54189283-54189302 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 54185553 | 54192880 | 54185553 | ID=MsG0380014401.01;Name=MsG0380014401.01 |
| Chr3 | mRNA | 54185553 | 54192880 | 54185553 | ID=MsG0380014401.01.T01;Parent=MsG0380014401.01;Name=MsG0380014401.01.T01;_AED=0.15;_eAED=0.15;_QI=835|1|1|1|0.75|0.8|5|3564|245 |
| Chr3 | exon | 54187791 | 54187967 | 54187791 | ID=MsG0380014401.01.T01:exon:10891;Parent=MsG0380014401.01.T01 |
| Chr3 | exon | 54188219 | 54188771 | 54188219 | ID=MsG0380014401.01.T01:exon:10892;Parent=MsG0380014401.01.T01 |
| Chr3 | exon | 54188871 | 54189036 | 54188871 | ID=MsG0380014401.01.T01:exon:10893;Parent=MsG0380014401.01.T01 |
| Chr3 | exon | 54189124 | 54192880 | 54189124 | ID=MsG0380014401.01.T01:exon:10894;Parent=MsG0380014401.01.T01 |
| Chr3 | five_prime_UTR | 54185553 | 54186036 | 54185553 | ID=MsG0380014401.01.T01:five_prime_utr;Parent=MsG0380014401.01.T01 |
| Chr3 | five_prime_UTR | 54187791 | 54187967 | 54187791 | ID=MsG0380014401.01.T01:five_prime_utr;Parent=MsG0380014401.01.T01 |
| Chr3 | five_prime_UTR | 54188219 | 54188392 | 54188219 | ID=MsG0380014401.01.T01:five_prime_utr;Parent=MsG0380014401.01.T01 |
| Chr3 | CDS | 54188393 | 54188771 | 54188393 | ID=MsG0380014401.01.T01:cds;Parent=MsG0380014401.01.T01 |
| Chr3 | CDS | 54188871 | 54189036 | 54188871 | ID=MsG0380014401.01.T01:cds;Parent=MsG0380014401.01.T01 |
| Chr3 | CDS | 54189124 | 54189316 | 54189124 | ID=MsG0380014401.01.T01:cds;Parent=MsG0380014401.01.T01 |
| Chr3 | three_prime_UTR | 54189317 | 54192880 | 54189317 | ID=MsG0380014401.01.T01:three_prime_utr;Parent=MsG0380014401.01.T01 |
| Chr3 | mRNA | 54185553 | 54188015 | 54185553 | ID=MsG0380014401.01.T02;Parent=MsG0380014401.01;Name=MsG0380014401.01.T02;_AED=0.45;_eAED=0.49;_QI=226|1|0.5|1|1|1|2|0|160 |
| Chr3 | exon | 54187791 | 54188015 | 54187791 | ID=MsG0380014401.01.T02:exon:10895;Parent=MsG0380014401.01.T02 |
| Chr3 | five_prime_UTR | 54185553 | 54185778 | 54185553 | ID=MsG0380014401.01.T02:five_prime_utr;Parent=MsG0380014401.01.T02 |
| Chr3 | CDS | 54185779 | 54186036 | 54185779 | ID=MsG0380014401.01.T02:cds;Parent=MsG0380014401.01.T02 |
| Chr3 | CDS | 54187791 | 54188015 | 54187791 | ID=MsG0380014401.01.T02:cds;Parent=MsG0380014401.01.T02 |
| Gene Sequence |
>MsG0380014401.01.T02 ATGGACGCCGCCGGCGATACCCAATTCCGGCGACAATCTCCCGCCGGCGAAATGGAACATCATTCTGAAGATCCAAATCGAACCGGGTCAGCTTCACGACCCGATTCTGTTTCTGACGGACCTACAACCTCCGGAGCTAGATACAAGCTGATGTCGCCGGCGAAGCTTCCGATCTCCCGGTCGCCGGTTTTGACGATTCCGCCGGGACTTAGTCCGACTTCGTTTCTTGAATCACCTGTTCTTCTCTCAAATATGAAGGTAGAACCTTCACCTACAACAGGCTCCCTTCCTAGGCTTCAACAAACAGTACATGGTTTTGTAACTTCTTCTACGTCCGCTGCGTTTCCTGTAACCTCTGCGTGCTTCAATTCTAATTCGGTTGACGACGGAAAATCCAGCTTCTTTGAGTTCAAACCGCACAGTAGATCAAATATGGTAATGGACTTGAGTTTGATTTTTTCACCCTTCTTTAATTTTACCTGA >MsG0380014401.01.T01 ATGTTGAAGTCGATTTTGGACGAATCAAATCCCAGTGGCAGCAAAGCAACTGGGCTTCAAGTATCACAAGTTGATGGTAGGGGCAGTGGACTTTCAGTTGCTGCCGATAAGGCATCTGATGATGGATACAATTGGCGAAAATATGGGCAGAAGCTTGTTAAAGGATGTGAATTTCCACGCAGTTATTACAAATGCACACATCCTAACTGTGAAGTAAAGAAACTCTTCGAACGCTCTCATGACGGCCAAATTACTGAGATAGTTTATAAGGGAACACATGATCATCCTAAACCTCAACCAAGCCGTCGATTCTCTGGTGGAAATATGATGTCTGTGCAAGAAGAGAGGTCTGATAGGGCTTCTTTAACTAGCCGAGACGACAAAGGCTTTAATAATTATGGTCAGGTGTCTCATGCAGCTGAGCGTGACAGTACTCCTGAGCTATCGCCTATAGCAGCAAATGATGGTAGTCCAGAGGGTGCAGGGTTTTTGTCAAACCAGAATAATGATGAGGTTGATGAAGATGATCCTTTCTCAAAGAGAAGAAAGATGGATCTTGACATCACACCGGTAGTTAAGCCTATCCGGGAGCCACGGGTCGTTGTACAAACTCTGAGTGAGGTTGACATATTGGATGATGGTTATCGCTGGCGTAAATATGGGCAGAAGGTGGTAAGAGGCAATCCTAACCCTAGGTATGTCCTCACATTGATGACTATACAATATAAACTAAATTGA |
| Protein sequence |
>MsG0380014401.01.T02 MDAAGDTQFRRQSPAGEMEHHSEDPNRTGSASRPDSVSDGPTTSGARYKLMSPAKLPISRSPVLTIPPGLSPTSFLESPVLLSNMKVEPSPTTGSLPRLQQTVHGFVTSSTSAAFPVTSACFNSNSVDDGKSSFFEFKPHSRSNMVMDLSLIFSPFFNFT* >MsG0380014401.01.T01 MLKSILDESNPSGSKATGLQVSQVDGRGSGLSVAADKASDDGYNWRKYGQKLVKGCEFPRSYYKCTHPNCEVKKLFERSHDGQITEIVYKGTHDHPKPQPSRRFSGGNMMSVQEERSDRASLTSRDDKGFNNYGQVSHAAERDSTPELSPIAANDGSPEGAGFLSNQNNDEVDEDDPFSKRRKMDLDITPVVKPIREPRVVVQTLSEVDILDDGYRWRKYGQKVVRGNPNPRYVLTLMTIQYKLN* |
