AlfalfaGEDB | MsG0580027899.01

M. sativa cultivar ZhongmuNo.1 / MsG0580027899.01

KEGG: -
GO: -
PFAM: PF16913

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027899.01.T01	XP_003615204.1	98.907	366	2	1	1	366	1	364	0	730

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027899.01.T01	Q9FZ95	55.49	337	143	2	17	353	1	330	6.15E-137	397

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027899.01.T01	G7KBC7	98.907	366	2	1	1	366	1	364	0.0	730

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048085.01	MsG0580027899.01	0.806454	8.162482e-50	4.432300e-47
MsG0080048505.01	MsG0580027899.01	0.802772	4.786821e-49	2.364975e-46
MsG0080048748.01	MsG0580027899.01	0.834372	3.113228e-56	3.687466e-53
MsG0080048773.01	MsG0580027899.01	0.802517	5.404529e-49	2.652623e-46
MsG0080048845.01	MsG0580027899.01	0.860123	2.571490e-63	6.954429e-60
MsG0080048849.01	MsG0580027899.01	0.802626	5.131257e-49	2.525608e-46
MsG0080048912.01	MsG0580027899.01	0.820788	5.699394e-53	4.553596e-50
MsG0080048938.01	MsG0580027899.01	0.884060	2.647064e-71	1.720869e-67
MsG0080048969.01	MsG0580027899.01	0.801119	1.046644e-48	4.958452e-46
MsG0080049029.01	MsG0580027899.01	0.806848	6.739557e-50	3.696857e-47

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PPI

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Gene1	Gene2	Type
MsG0380017035.01	MsG0580027899.01	PPI
MsG0580027899.01	MsG0380017034.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027899.01.T01	MTR_5g065080	98.907	366	2	1	1	366	1	364	0.0	730
MsG0580027899.01.T01	MTR_3g099740	63.760	367	123	4	7	365	4	368	3.58e-169	476
MsG0580027899.01.T01	MTR_3g099750	61.096	365	132	4	9	365	5	367	1.45e-160	455
MsG0580027899.01.T01	MTR_2g437590	36.517	356	200	7	12	358	65	403	3.62e-70	226
MsG0580027899.01.T01	MTR_2g015470	33.134	335	216	2	22	356	44	370	1.49e-58	194
MsG0580027899.01.T01	MTR_4g098900	33.828	337	197	5	27	360	42	355	6.97e-57	191
MsG0580027899.01.T01	MTR_3g058660	33.333	360	225	5	12	366	7	356	2.59e-56	188
MsG0580027899.01.T01	MTR_2g437580	34.891	321	188	7	41	355	72	377	9.17e-53	179
MsG0580027899.01.T01	MTR_8g024620	32.344	337	201	3	20	350	42	357	4.29e-52	177
MsG0580027899.01.T01	MTR_8g024620	32.344	337	201	3	20	350	53	368	4.75e-52	177

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580027899.01.T01	AT2G33750	58.721	344	135	2	17	360	3	339	4.00e-141	404
MsG0580027899.01.T01	AT1G28220	55.490	337	143	2	17	353	1	330	6.27e-138	397
MsG0580027899.01.T01	AT2G33750	56.901	355	135	3	17	360	3	350	1.83e-137	395
MsG0580027899.01.T01	AT1G28230	58.621	348	138	3	17	359	1	347	1.15e-135	391
MsG0580027899.01.T01	AT4G18220	35.404	322	194	5	41	361	66	374	2.03e-56	189
MsG0580027899.01.T01	AT4G18210	32.964	361	221	5	7	361	31	376	1.07e-52	179
MsG0580027899.01.T01	AT4G18205	34.891	321	192	4	41	357	61	368	2.71e-51	175
MsG0580027899.01.T01	AT4G18197	32.722	327	203	4	41	363	64	377	8.37e-46	161
MsG0580027899.01.T01	AT1G30840	33.923	339	206	6	8	342	12	336	2.58e-45	160
MsG0580027899.01.T01	AT1G30840	33.923	339	206	6	8	342	12	336	2.58e-45	160

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Find 77 sgRNAs with CRISPR-Local

Find 140 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATAATTAACCAGCCATGG+TGG	0.679882	5:+70655844	MsG0580027899.01.T01:exon
AACAGCTAAGATTTCAGTTA+TGG	0.309424	5:-70657840	None:intergenic
AACGGTAACGGTAACGGTTG+TGG	0.403687	5:-70656081	None:intergenic
AACTCAATCCTCTTAGCCAT+AGG	0.604730	5:+70655932	MsG0580027899.01.T01:CDS
AAGCAAGGGATTTCAAGCTT+GGG	0.461079	5:+70657690	MsG0580027899.01.T01:CDS
AAGTATAGACGCATTACTAG+TGG	0.634156	5:-70655968	None:intergenic
AATGCCAGATAGCAATGAAG+AGG	0.600270	5:-70657798	None:intergenic
ACAGCTAAGATTTCAGTTAT+GGG	0.401537	5:-70657839	None:intergenic
ACCACCTTTGTTTATCGCTT+CGG	0.421218	5:+70656153	MsG0580027899.01.T01:CDS
ACGGTTGTGGATGTGAGAAA+TGG	0.530168	5:-70656068	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACAGCTAAGATTTCAGTTA+TGG	-	Chr5:70657843-70657862	None:intergenic	30.0%
AATTGTGAGTGTACAAAGTA+TGG	-	Chr5:70656664-70656683	None:intergenic	30.0%
ACAGCTAAGATTTCAGTTAT+GGG	-	Chr5:70657842-70657861	None:intergenic	30.0%
AGAAATTTCAAGCAGAGAAA+GGG	+	Chr5:70657876-70657895	MsG0580027899.01.T01:CDS	30.0%
CTATTCTTAGATTCAATACG+TGG	-	Chr5:70657218-70657237	None:intergenic	30.0%
GGATTGAGTTTAGTATTAGA+AGG	-	Chr5:70655922-70655941	None:intergenic	30.0%
GTTAAGGGAATGAGAATTAT+TGG	-	Chr5:70656049-70656068	None:intergenic	30.0%
TAAAAAGAGAGCAATATTCG+GGG	-	Chr5:70657127-70657146	None:intergenic	30.0%
TCAACTAAGGATTATGTGAT+TGG	+	Chr5:70656415-70656434	MsG0580027899.01.T01:CDS	30.0%
TGTTTATAAGAGTGATTGGA+GGG	-	Chr5:70656802-70656821	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	70655812	70658140	70655812	ID=MsG0580027899.01;Name=MsG0580027899.01
Chr5	mRNA	70655812	70658140	70655812	ID=MsG0580027899.01.T01;Parent=MsG0580027899.01;Name=MsG0580027899.01.T01;_AED=0.33;_eAED=0.33;_QI=48\|1\|1\|1\|1\|1\|2\|122\|366
Chr5	exon	70655812	70656618	70655812	ID=MsG0580027899.01.T01:exon:5353;Parent=MsG0580027899.01.T01
Chr5	exon	70657677	70658140	70657677	ID=MsG0580027899.01.T01:exon:5354;Parent=MsG0580027899.01.T01
Chr5	five_prime_UTR	70655812	70655859	70655812	ID=MsG0580027899.01.T01:five_prime_utr;Parent=MsG0580027899.01.T01
Chr5	CDS	70655860	70656618	70655860	ID=MsG0580027899.01.T01:cds;Parent=MsG0580027899.01.T01
Chr5	CDS	70657677	70658018	70657677	ID=MsG0580027899.01.T01:cds;Parent=MsG0580027899.01.T01
Chr5	three_prime_UTR	70658019	70658140	70658019	ID=MsG0580027899.01.T01:three_prime_utr;Parent=MsG0580027899.01.T01

Gene Sequence

>MsG0580027899.01.T01

ATGGTGGAAAGAATAATTAGCAAAGAAGAAGAAAGCAAAAAAAGAAGCATGAAGAAACTCCTTCTAATACTAAACTCAATCCTCTTAGCCATAGGAACATGTGGTGGACCACTAGTAATGCGTCTATACTTCATCCATGGCGGCAAACGTGTATGGCTCTCAAGCTTCCTTGAAACATCTGCCTTCCCAATAATTCTCATTCCCTTAACCATTTCTCACATCCACAACCGTTACCGTTACCGTTACCAAAACCCTAACGGCAACAACAATAATAACAACTTTGTCTCAATGAAACCACCTTTGTTTATCGCTTCGGCAGTCATCGGAGTTCTCACCGGTTTAGACGACTACCTCTACGCCTGCGGCATAAAGCGTCTACCGGTGTCAACTTCTTCGTTAATTCAATCCTCACACTTAGCTTTCACCGCGGTTTTCGCGTTTTTTCTTGTGAAGCATAAGTTCACAGCTTATTCTGTTAACTCTATTGTTTTGTTGACCCTTGGGAGTGTTGTTTTGGCACTGAATTCTGATGGTGGAGATAGACTTGTTGGTGAGTCAACTAAGGATTATGTGATTGGTTTTGTTATGATATTGGCTTCTGCTGCTTTGTATGGATTTGTTTTACCGTTGTTGGAGTTGGTTTATAAAAAGAGTAAACAAGTTATTACTTATTCTCTTGTTATGGAGATTCAGCTTGTTATGTGCTTCTTTGCTACTTTGTTTTGCGTTGTTGGGATGATCATAGACAATGATTTTAAGGTGATTCCAAGGGAAGCAAGGGATTTCAAGCTTGGGGAAACAAAGTACTATGTTGTGCTAGTGTGGAGTGCTATAATGTGGCAATTTTTCTTCTTAGGAGCGATTGGAGTTATCTTTTGTGCCTCTTCATTGCTATCTGGCATTATAATTGCTGCATTTCTTCCCATAACTGAAATCTTAGCTGTTATTGTCTACAAAGAGAAATTTCAAGCAGAGAAAGGGGTTGCTTTGGTGCTCTCACTTTGGGGCTTTGTGTCTTACTTTTATGATGAGATTAAAGAAGCAAAAAAAATGAAAAATAGAGAAATGGAACTGCCTCAAAGTCTTCATTCAATCACATGA

Protein sequence

>MsG0580027899.01.T01

MVERIISKEEESKKRSMKKLLLILNSILLAIGTCGGPLVMRLYFIHGGKRVWLSSFLETSAFPIILIPLTISHIHNRYRYRYQNPNGNNNNNNFVSMKPPLFIASAVIGVLTGLDDYLYACGIKRLPVSTSSLIQSSHLAFTAVFAFFLVKHKFTAYSVNSIVLLTLGSVVLALNSDGGDRLVGESTKDYVIGFVMILASAALYGFVLPLLELVYKKSKQVITYSLVMEIQLVMCFFATLFCVVGMIIDNDFKVIPREARDFKLGETKYYVVLVWSAIMWQFFFLGAIGVIFCASSLLSGIIIAAFLPITEILAVIVYKEKFQAEKGVALVLSLWGFVSYFYDEIKEAKKMKNREMELPQSLHSIT*