AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280010728.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010728.01.T01 MTR_2g090330 96.894 161 3 1 1 161 5 163 1.73e-114 321
MsG0280010728.01.T01 MTR_2g090330 96.894 161 3 1 1 161 8 166 1.94e-114 321
MsG0280010728.01.T01 MTR_8g465910 58.475 118 45 1 18 131 34 151 1.29e-46 152
MsG0280010728.01.T01 MTR_2g090325 60.465 129 31 4 13 131 3 121 3.63e-45 147
MsG0280010728.01.T01 MTR_2g090295 64.545 110 29 2 25 124 37 146 9.09e-45 147
MsG0280010728.01.T01 MTR_2g013550 48.120 133 59 4 2 129 5 132 1.10e-32 116
MsG0280010728.01.T01 MTR_4g088870 42.446 139 71 5 2 133 7 143 3.49e-30 108
MsG0280010728.01.T01 MTR_4g088875 45.690 116 55 3 2 111 7 120 4.78e-29 107
MsG0280010728.01.T01 MTR_5g019300 48.889 90 41 2 26 115 33 117 2.94e-28 105
MsG0280010728.01.T01 MTR_1g021895 48.352 91 42 2 25 115 27 112 1.89e-27 102
MsG0280010728.01.T01 MTR_4g029440 46.667 90 44 1 26 115 124 209 4.93e-26 102
MsG0280010728.01.T01 MTR_4g029440 46.667 90 44 1 26 115 130 215 5.24e-26 102
MsG0280010728.01.T01 MTR_1g054260 41.176 102 52 3 22 115 78 179 1.70e-23 91.7
MsG0280010728.01.T01 MTR_1g054465 36.975 119 62 4 1 111 30 143 1.80e-23 91.7
MsG0280010728.01.T01 MTR_7g093390 43.182 88 43 2 28 115 170 250 2.28e-22 92.4
MsG0280010728.01.T01 MTR_1g054210 41.000 100 49 3 28 124 122 214 2.87e-22 89.4
MsG0280010728.01.T01 MTR_1g054280 42.857 84 44 2 28 111 76 155 2.34e-20 85.1
MsG0280010728.01.T01 MTR_2g061230 38.889 90 51 1 26 115 105 190 1.89e-19 82.0
MsG0280010728.01.T01 MTR_1g015390 44.068 59 26 1 29 87 365 416 2.80e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280010728.01.T01 AT4G03510 56.989 93 31 2 26 114 44 131 4.98e-33 117
MsG0280010728.01.T01 AT4G03510 56.989 93 31 2 26 114 44 131 4.98e-33 117
MsG0280010728.01.T01 AT4G03510 56.989 93 31 2 26 114 44 131 4.98e-33 117
MsG0280010728.01.T01 AT4G03510 56.989 93 31 2 26 114 44 131 4.98e-33 117
MsG0280010728.01.T01 AT4G28270 48.305 118 43 5 25 133 16 124 6.53e-32 113
MsG0280010728.01.T01 AT1G74990 46.667 105 47 3 25 129 14 109 4.59e-29 104
MsG0280010728.01.T01 AT2G23780 46.465 99 47 3 17 115 16 108 4.00e-27 102
MsG0280010728.01.T01 AT2G23780 46.465 99 47 3 17 115 16 108 4.00e-27 102
MsG0280010728.01.T01 AT2G23780 46.465 99 47 3 17 115 16 108 4.00e-27 102
MsG0280010728.01.T01 AT1G19310 51.136 88 38 2 28 115 21 103 1.06e-26 100
MsG0280010728.01.T01 AT4G27470 48.598 107 46 5 28 127 42 146 2.16e-26 100
MsG0280010728.01.T01 AT3G58030 46.341 82 34 2 9 90 123 194 4.37e-22 91.7
MsG0280010728.01.T01 AT3G58030 46.341 82 34 2 9 90 123 194 4.37e-22 91.7
MsG0280010728.01.T01 AT3G58030 46.341 82 34 2 9 90 123 194 4.37e-22 91.7
MsG0280010728.01.T01 AT3G58030 46.341 82 34 2 9 90 123 194 4.37e-22 91.7
MsG0280010728.01.T01 AT2G42030 46.067 89 40 2 28 116 139 219 7.64e-22 90.9
MsG0280010728.01.T01 AT2G44410 37.500 88 51 1 28 115 123 206 8.37e-18 79.7

Find 37 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCGGAAATCAAGCTTATA+TGG 0.186494 2:+73980699 None:intergenic
GAAATTTCTGATTTGCATAT+TGG 0.222866 2:+73980939 None:intergenic
ATGATATTCAATCAAATTTC+CGG 0.230037 2:+73980682 None:intergenic
GATTTGCATATTGGACATTC+TGG 0.274453 2:+73980948 None:intergenic
GCTTCTGCTTGTTCTAGTTC+AGG 0.300850 2:+73980780 None:intergenic
TTGCACATTTGCTTACAATA+TGG 0.306524 2:-73980723 MsG0280010728.01.T01:CDS
GCCCATATAAGCTTGATTTC+CGG 0.309807 2:-73980701 MsG0280010728.01.T01:CDS
TCAACACTTGTTCCACTATA+TGG 0.312394 2:-73980912 MsG0280010728.01.T01:CDS
AGCAGAAGCACCACCAGCAC+AGG 0.376264 2:-73980754 MsG0280010728.01.T01:CDS
TTTGTGGTCATCTCTATTGT+TGG 0.402478 2:-73981022 MsG0280010728.01.T01:CDS
TAATGGACATTCTGTCGAAC+CGG 0.412747 2:-73981154 MsG0280010728.01.T01:CDS
GTTGGCCTTGTATTTACAAA+TGG 0.435974 2:-73981004 MsG0280010728.01.T01:CDS
TCCGGAAATCAAGCTTATAT+GGG 0.455288 2:+73980700 None:intergenic
GTTGCAGTATCAAGTGATCT+TGG 0.469495 2:+73980813 None:intergenic
GATCCAGTGGTAACACTTTG+TGG 0.471364 2:-73981038 MsG0280010728.01.T01:CDS
GATCTTGGACCAAGTGGTCT+TGG 0.481662 2:+73980828 None:intergenic
GTTTCACCACGACCATATAG+TGG 0.492063 2:+73980900 None:intergenic
GAAGGTGAGGCTCATCAAGA+TGG 0.515188 2:-73980864 MsG0280010728.01.T01:CDS
AATGGACATTCTGTCGAACC+GGG 0.527440 2:-73981153 MsG0280010728.01.T01:CDS
TTAATCCATTTGTAAATACA+AGG 0.530718 2:+73980999 None:intergenic
CTTGGACCAAGTGGTCTTGG+TGG 0.541843 2:+73980831 None:intergenic
CTCACCTTCAGAACTTGATG+TGG 0.546869 2:+73980878 None:intergenic
AATGGATTAATAGCACCTCA+TGG 0.547502 2:-73980986 MsG0280010728.01.T01:CDS
CATTAGCATCAGCTTTGCCC+CGG 0.566386 2:+73981135 None:intergenic
TGCAAACTACTCTCCTGTGC+TGG 0.567244 2:+73980741 None:intergenic
AAGGTGAGGCTCATCAAGAT+GGG 0.576426 2:-73980863 MsG0280010728.01.T01:CDS
CACATCAAGTTCTGAAGGTG+AGG 0.577593 2:-73980877 MsG0280010728.01.T01:CDS
AAACTACTCTCCTGTGCTGG+TGG 0.580631 2:+73980744 None:intergenic
GTTGTACCACCAAGACCACT+TGG 0.583553 2:-73980837 MsG0280010728.01.T01:CDS
ATGGATTAATAGCACCTCAT+GGG 0.600842 2:-73980985 MsG0280010728.01.T01:CDS
TGACCACAAAGTGTTACCAC+TGG 0.610639 2:+73981035 None:intergenic
GAAACCACATCAAGTTCTGA+AGG 0.614193 2:-73980882 MsG0280010728.01.T01:CDS
CTTGTTCCACTATATGGTCG+TGG 0.622216 2:-73980906 MsG0280010728.01.T01:CDS
TTTCTCATTATGTTCCCATG+AGG 0.630818 2:+73980971 None:intergenic
TCAAGTGATCTTGGACCAAG+TGG 0.644730 2:+73980822 None:intergenic
GGAGCATGTGAAAGATCCAG+TGG 0.680623 2:-73981051 MsG0280010728.01.T01:CDS
ATGGACATTCTGTCGAACCG+GGG 0.697290 2:-73981152 MsG0280010728.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATCCATTTGTAAATACA+AGG + Chr2:73980845-73980864 None:intergenic 20.0%
!! GAAATTTCTGATTTGCATAT+TGG + Chr2:73980905-73980924 None:intergenic 25.0%
!! TTTTGAATGCAACATATGTT+TGG - Chr2:73980769-73980788 MsG0280010728.01.T01:CDS 25.0%
TTGCACATTTGCTTACAATA+TGG - Chr2:73981118-73981137 MsG0280010728.01.T01:CDS 30.0%
GATTTGCATATTGGACATTC+TGG + Chr2:73980896-73980915 None:intergenic 35.0%
TCAACACTTGTTCCACTATA+TGG - Chr2:73980929-73980948 MsG0280010728.01.T01:CDS 35.0%
TCCGGAAATCAAGCTTATAT+GGG + Chr2:73981144-73981163 None:intergenic 35.0%
TTCCGGAAATCAAGCTTATA+TGG + Chr2:73981145-73981164 None:intergenic 35.0%
TTTCTCATTATGTTCCCATG+AGG + Chr2:73980873-73980892 None:intergenic 35.0%
TTTGTGGTCATCTCTATTGT+TGG - Chr2:73980819-73980838 MsG0280010728.01.T01:CDS 35.0%
! ATGGATTAATAGCACCTCAT+GGG - Chr2:73980856-73980875 MsG0280010728.01.T01:CDS 35.0%
!! AATGGATTAATAGCACCTCA+TGG - Chr2:73980855-73980874 MsG0280010728.01.T01:CDS 35.0%
!! GTTGGCCTTGTATTTACAAA+TGG - Chr2:73980837-73980856 MsG0280010728.01.T01:CDS 35.0%
GCCCATATAAGCTTGATTTC+CGG - Chr2:73981140-73981159 MsG0280010728.01.T01:CDS 40.0%
GTTGCAGTATCAAGTGATCT+TGG + Chr2:73981031-73981050 None:intergenic 40.0%
! GAAACCACATCAAGTTCTGA+AGG - Chr2:73980959-73980978 MsG0280010728.01.T01:CDS 40.0%
AAGGTGAGGCTCATCAAGAT+GGG - Chr2:73980978-73980997 MsG0280010728.01.T01:CDS 45.0%
CTCACCTTCAGAACTTGATG+TGG + Chr2:73980966-73980985 None:intergenic 45.0%
CTTGTTCCACTATATGGTCG+TGG - Chr2:73980935-73980954 MsG0280010728.01.T01:CDS 45.0%
GATCCAGTGGTAACACTTTG+TGG - Chr2:73980803-73980822 MsG0280010728.01.T01:CDS 45.0%
GTTTCACCACGACCATATAG+TGG + Chr2:73980944-73980963 None:intergenic 45.0%
TCAAGTGATCTTGGACCAAG+TGG + Chr2:73981022-73981041 None:intergenic 45.0%
! TGACCACAAAGTGTTACCAC+TGG + Chr2:73980809-73980828 None:intergenic 45.0%
!! CACATCAAGTTCTGAAGGTG+AGG - Chr2:73980964-73980983 MsG0280010728.01.T01:CDS 45.0%
!! GCTTCTGCTTGTTCTAGTTC+AGG + Chr2:73981064-73981083 None:intergenic 45.0%
AAACTACTCTCCTGTGCTGG+TGG + Chr2:73981100-73981119 None:intergenic 50.0%
ATGGACATTCTGTCGAACCG+GGG - Chr2:73980689-73980708 MsG0280010728.01.T01:CDS 50.0%
CATTAGCATCAGCTTTGCCC+CGG + Chr2:73980709-73980728 None:intergenic 50.0%
GAAGGTGAGGCTCATCAAGA+TGG - Chr2:73980977-73980996 MsG0280010728.01.T01:CDS 50.0%
GATCTTGGACCAAGTGGTCT+TGG + Chr2:73981016-73981035 None:intergenic 50.0%
GGAGCATGTGAAAGATCCAG+TGG - Chr2:73980790-73980809 MsG0280010728.01.T01:CDS 50.0%
GTTGTACCACCAAGACCACT+TGG - Chr2:73981004-73981023 MsG0280010728.01.T01:CDS 50.0%
TGCAAACTACTCTCCTGTGC+TGG + Chr2:73981103-73981122 None:intergenic 50.0%
! CTTGGACCAAGTGGTCTTGG+TGG + Chr2:73981013-73981032 None:intergenic 55.0%
!! AGCAGAAGCACCACCAGCAC+AGG - Chr2:73981087-73981106 MsG0280010728.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr2 gene 73980689 73981174 73980689 ID=MsG0280010728.01;Name=MsG0280010728.01
Chr2 mRNA 73980689 73981174 73980689 ID=MsG0280010728.01.T01;Parent=MsG0280010728.01;Name=MsG0280010728.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|161
Chr2 exon 73980689 73981174 73980689 ID=MsG0280010728.01.T01:exon:7427;Parent=MsG0280010728.01.T01
Chr2 CDS 73980689 73981174 73980689 ID=MsG0280010728.01.T01:cds;Parent=MsG0280010728.01.T01
Gene Sequence

>MsG0280010728.01.T01

ATGGACATTCTGTCGAACCGGGGCAAAGCTGATGCTAATGCTAATGCTAATGCAGATTCTAATAAAATCTGTTCTAATGATTTTGAATGCAACATATGTTTGGAGCATGTGAAAGATCCAGTGGTAACACTTTGTGGTCATCTCTATTGTTGGCCTTGTATTTACAAATGGATTAATAGCACCTCATGGGAACATAATGAGAAACCAGAATGTCCAATATGCAAATCAGAAATTTCAGAATCAACACTTGTTCCACTATATGGTCGTGGTGAAACCACATCAAGTTCTGAAGGTGAGGCTCATCAAGATGGGAATGTTGTACCACCAAGACCACTTGGTCCAAGATCACTTGATACTGCAACTGTTTCACAACCTGAACTAGAACAAGCAGAAGCAGAAGCAGAAGCACCACCAGCACAGGAGAGTAGTTTGCACATTTGCTTACAATATGGCCCATATAAGCTTGATTTCCGGAAATTTGATTGA

Protein sequence

>MsG0280010728.01.T01

MDILSNRGKADANANANADSNKICSNDFECNICLEHVKDPVVTLCGHLYCWPCIYKWINSTSWEHNEKPECPICKSEISESTLVPLYGRGETTSSSEGEAHQDGNVVPPRPLGPRSLDTATVSQPELEQAEAEAEAPPAQESSLHICLQYGPYKLDFRKFD*