AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019285.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019285.01.T01 MTR_4g027800 88.496 113 13 0 1 113 1 113 2.62e-67 199
MsG0480019285.01.T01 MTR_4g027840 89.381 113 12 0 1 113 1 113 2.68e-67 199
MsG0480019285.01.T01 MTR_4g428370 84.071 113 18 0 1 113 58 170 1.91e-64 194
MsG0480019285.01.T01 MTR_4g029390 66.372 113 35 2 1 111 1 112 1.10e-45 144
MsG0480019285.01.T01 MTR_4g029350 66.372 113 35 2 1 111 1 112 7.44e-45 142
MsG0480019285.01.T01 MTR_7g072930 46.923 130 60 3 4 125 7 135 1.52e-36 122
MsG0480019285.01.T01 MTR_4g029210 50.000 112 56 0 1 112 1 112 5.38e-32 109
MsG0480019285.01.T01 MTR_4g028190 47.458 118 60 2 1 116 5 122 7.06e-32 109
MsG0480019285.01.T01 MTR_7g073150 47.368 114 56 3 12 122 14 126 9.24e-31 107
MsG0480019285.01.T01 MTR_7g072730 40.323 124 71 3 4 124 7 130 9.06e-29 103
MsG0480019285.01.T01 MTR_7g073170 43.636 110 58 3 4 111 7 114 2.64e-26 95.5
MsG0480019285.01.T01 MTR_7g072830 40.000 110 64 1 4 111 7 116 1.23e-25 93.6
MsG0480019285.01.T01 MTR_7g072960 42.149 121 48 4 10 122 12 118 6.96e-25 92.0
MsG0480019285.01.T01 MTR_7g073120 37.302 126 77 1 1 124 5 130 1.31e-24 92.0
MsG0480019285.01.T01 MTR_7g072993 41.935 124 69 1 4 124 7 130 1.83e-24 91.3
MsG0480019285.01.T01 MTR_4g028180 42.017 119 61 4 5 116 9 126 2.91e-24 90.5
MsG0480019285.01.T01 MTR_4g028310 40.000 115 66 1 1 112 1 115 3.00e-23 88.2
MsG0480019285.01.T01 MTR_2g062600 43.689 103 56 2 11 112 11 112 9.09e-23 86.3
MsG0480019285.01.T01 MTR_7g072980 37.398 123 75 1 4 124 7 129 9.87e-22 84.3
MsG0480019285.01.T01 MTR_7g072900 43.243 111 60 2 4 112 7 116 1.54e-21 83.2
MsG0480019285.01.T01 MTR_7g073130 40.517 116 65 4 4 117 7 120 5.15e-21 82.0
MsG0480019285.01.T01 MTR_4g028250 40.351 114 65 2 1 111 1 114 2.40e-19 77.8
MsG0480019285.01.T01 MTR_7g073100 37.600 125 71 2 4 124 7 128 4.10e-19 77.8
MsG0480019285.01.T01 MTR_3g467100 37.963 108 66 1 5 111 3 110 1.02e-18 75.9
MsG0480019285.01.T01 MTR_7g072810 37.903 124 69 3 4 125 7 124 6.23e-18 74.3
MsG0480019285.01.T01 MTR_1g040335 42.727 110 60 2 5 112 8 116 2.21e-17 72.8
MsG0480019285.01.T01 MTR_7g451500 38.202 89 54 1 24 111 7 95 4.73e-17 71.2
MsG0480019285.01.T01 MTR_7g073030 35.772 123 76 3 4 124 7 128 7.33e-17 72.4
MsG0480019285.01.T01 MTR_7g073060 37.838 111 66 3 4 112 7 116 7.94e-16 68.6
MsG0480019285.01.T01 MTR_4g028360 34.211 114 72 3 4 115 1 113 2.23e-15 67.4
MsG0480019285.01.T01 MTR_3g056640 34.545 110 66 3 9 113 12 120 1.90e-12 60.1
MsG0480019285.01.T01 MTR_3g046350 31.373 102 64 3 10 106 13 113 4.26e-12 58.9
MsG0480019285.01.T01 MTR_3g046515 33.333 111 68 4 4 110 11 119 7.85e-12 58.2
MsG0480019285.01.T01 MTR_7g072987 48.529 68 32 2 25 90 57 123 8.83e-12 59.3
MsG0480019285.01.T01 MTR_3g464130 36.667 90 52 3 25 110 18 106 2.68e-11 56.6
MsG0480019285.01.T01 MTR_3g046530 30.435 115 74 3 4 113 7 120 3.11e-11 57.0
MsG0480019285.01.T01 MTR_1g040330 36.937 111 65 4 5 112 8 116 5.02e-11 56.2
MsG0480019285.01.T01 MTR_3g046540 35.556 90 53 3 25 110 31 119 5.13e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480019285.01.T01 AT2G38540 63.158 114 36 6 4 113 5 116 2.65e-36 120
MsG0480019285.01.T01 AT5G59320 59.615 104 40 1 8 111 10 111 2.70e-36 120
MsG0480019285.01.T01 AT5G59310 59.434 106 38 2 8 113 10 110 6.14e-35 117
MsG0480019285.01.T01 AT2G38530 59.292 113 42 4 4 113 5 116 7.94e-32 109
MsG0480019285.01.T01 AT3G51600 52.212 113 50 4 4 113 5 116 3.68e-28 100
MsG0480019285.01.T01 AT2G15050 44.068 118 60 4 4 115 5 122 3.54e-27 97.8
MsG0480019285.01.T01 AT3G51590 45.794 107 55 3 6 111 8 112 6.25e-26 94.4
MsG0480019285.01.T01 AT2G15050 43.966 116 59 4 4 113 5 120 1.25e-25 94.0
MsG0480019285.01.T01 AT2G15050 44.037 109 57 3 4 108 5 113 7.22e-25 91.7
MsG0480019285.01.T01 AT4G33355 50.562 89 44 0 23 111 27 115 1.05e-24 91.3
MsG0480019285.01.T01 AT4G33355 50.562 89 44 0 23 111 27 115 1.05e-24 91.3
MsG0480019285.01.T01 AT5G01870 44.545 110 53 4 9 111 4 112 1.01e-23 89.0
MsG0480019285.01.T01 AT3G08770 45.263 95 48 3 22 113 18 111 5.92e-19 76.6
MsG0480019285.01.T01 AT3G08770 46.237 93 46 3 22 111 18 109 7.88e-19 76.3
MsG0480019285.01.T01 AT2G18370 41.489 94 53 2 21 113 22 114 1.14e-17 73.2
MsG0480019285.01.T01 AT2G15325 33.708 89 56 2 27 113 31 118 3.22e-11 56.6

Find 33 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGCTGCTGGTGCTATTTC+TGG 0.136175 4:+17750296 MsG0480019285.01.T01:CDS
CCATGCATTGGATATCTTAG+AGG 0.354250 4:+17750173 MsG0480019285.01.T01:CDS
GCTATTTCTGGTTTGAATAC+AGG 0.359485 4:+17750308 MsG0480019285.01.T01:CDS
TTGCAAGCAGCCTGGCGGTC+AGG 0.369068 4:-17750263 None:intergenic
GTCAGGCGTGGTGGTGGCGG+CGG 0.430334 4:-17750246 None:intergenic
GCGGTCAGGCGTGGTGGTGG+CGG 0.445777 4:-17750249 None:intergenic
CTGGCGGTCAGGCGTGGTGG+TGG 0.447269 4:-17750252 None:intergenic
CACCACCACGCCTGACCGCC+AGG 0.460254 4:+17750253 MsG0480019285.01.T01:CDS
GTGCATGATTGTTGCACCTA+TGG 0.467928 4:+17750106 MsG0480019285.01.T01:CDS
GGTGCTCTTTCTCCATGCAT+TGG 0.474474 4:+17750161 MsG0480019285.01.T01:CDS
AGCAGCCTGGCGGTCAGGCG+TGG 0.477818 4:-17750258 None:intergenic
AACTGCTTGAAAAGTGCTGC+TGG 0.486030 4:+17750284 MsG0480019285.01.T01:CDS
TCAAGCAGTTGCAAGCAGCC+TGG 0.486116 4:-17750271 None:intergenic
TCTTCTCTCCCTGGCAAATG+TGG 0.490642 4:+17750338 MsG0480019285.01.T01:CDS
TTCACTCCACCACAGCATGC+TGG 0.495886 4:-17750212 None:intergenic
GGATATCTTAGAGGTGGTGC+TGG 0.504129 4:+17750182 MsG0480019285.01.T01:CDS
GTGGAGGTACTGATCTTGTA+AGG 0.508470 4:-17750371 None:intergenic
ATGTTAACACCACATTTGCC+AGG 0.518576 4:-17750347 None:intergenic
GGCGCAGCTTCTTCTCTCCC+TGG 0.542091 4:+17750329 MsG0480019285.01.T01:CDS
CCACCACAGCATGCTGGAGA+TGG 0.543598 4:-17750206 None:intergenic
CCATCTCCAGCATGCTGTGG+TGG 0.550747 4:+17750206 MsG0480019285.01.T01:CDS
GGTCCATCTCCAGCATGCTG+TGG 0.560551 4:+17750203 MsG0480019285.01.T01:CDS
AGCCTGGCGGTCAGGCGTGG+TGG 0.562191 4:-17750255 None:intergenic
AACATACGTAGCACAGTTGG+TGG 0.566288 4:-17750390 None:intergenic
GAGATTGCAGCATCTGCCAT+AGG 0.577723 4:-17750122 None:intergenic
CCTCTAAGATATCCAATGCA+TGG 0.598915 4:-17750173 None:intergenic
ATGAACATACGTAGCACAGT+TGG 0.601791 4:-17750393 None:intergenic
TGCATTGGATATCTTAGAGG+TGG 0.623803 4:+17750176 MsG0480019285.01.T01:CDS
ATACGTAGCACAGTTGGTGG+AGG 0.639269 4:-17750387 None:intergenic
TGTTAACACCACATTTGCCA+GGG 0.642773 4:-17750346 None:intergenic
AGCAGTTGCAAGCAGCCTGG+CGG 0.651296 4:-17750268 None:intergenic
GCAGATGCTGCAATCTCATG+TGG 0.654132 4:+17750128 MsG0480019285.01.T01:CDS
ATCTCATGTGGACAAGTGAG+TGG 0.724614 4:+17750140 MsG0480019285.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTATTTCTGGTTTGAATAC+AGG + Chr4:17750308-17750327 MsG0480019285.01.T01:CDS 35.0%
ATGAACATACGTAGCACAGT+TGG - Chr4:17750396-17750415 None:intergenic 40.0%
ATGTTAACACCACATTTGCC+AGG - Chr4:17750350-17750369 None:intergenic 40.0%
CCATGCATTGGATATCTTAG+AGG + Chr4:17750173-17750192 MsG0480019285.01.T01:CDS 40.0%
TGCATTGGATATCTTAGAGG+TGG + Chr4:17750176-17750195 MsG0480019285.01.T01:CDS 40.0%
TGTTAACACCACATTTGCCA+GGG - Chr4:17750349-17750368 None:intergenic 40.0%
!! CCTCTAAGATATCCAATGCA+TGG - Chr4:17750176-17750195 None:intergenic 40.0%
AACATACGTAGCACAGTTGG+TGG - Chr4:17750393-17750412 None:intergenic 45.0%
ATCTCATGTGGACAAGTGAG+TGG + Chr4:17750140-17750159 MsG0480019285.01.T01:CDS 45.0%
GTGCATGATTGTTGCACCTA+TGG + Chr4:17750106-17750125 MsG0480019285.01.T01:CDS 45.0%
!! AACTGCTTGAAAAGTGCTGC+TGG + Chr4:17750284-17750303 MsG0480019285.01.T01:CDS 45.0%
!! GTGGAGGTACTGATCTTGTA+AGG - Chr4:17750374-17750393 None:intergenic 45.0%
GAGATTGCAGCATCTGCCAT+AGG - Chr4:17750125-17750144 None:intergenic 50.0%
GCAGATGCTGCAATCTCATG+TGG + Chr4:17750128-17750147 MsG0480019285.01.T01:CDS 50.0%
GGATATCTTAGAGGTGGTGC+TGG + Chr4:17750182-17750201 MsG0480019285.01.T01:CDS 50.0%
GGTGCTCTTTCTCCATGCAT+TGG + Chr4:17750161-17750180 MsG0480019285.01.T01:CDS 50.0%
TCTTCTCTCCCTGGCAAATG+TGG + Chr4:17750338-17750357 MsG0480019285.01.T01:CDS 50.0%
!! AGTGCTGCTGGTGCTATTTC+TGG + Chr4:17750296-17750315 MsG0480019285.01.T01:CDS 50.0%
!! ATACGTAGCACAGTTGGTGG+AGG - Chr4:17750390-17750409 None:intergenic 50.0%
TCAAGCAGTTGCAAGCAGCC+TGG - Chr4:17750274-17750293 None:intergenic 55.0%
TTCACTCCACCACAGCATGC+TGG - Chr4:17750215-17750234 None:intergenic 55.0%
AGCAGTTGCAAGCAGCCTGG+CGG - Chr4:17750271-17750290 None:intergenic 60.0%
CCACCACAGCATGCTGGAGA+TGG - Chr4:17750209-17750228 None:intergenic 60.0%
CCATCTCCAGCATGCTGTGG+TGG + Chr4:17750206-17750225 MsG0480019285.01.T01:CDS 60.0%
GGTCCATCTCCAGCATGCTG+TGG + Chr4:17750203-17750222 MsG0480019285.01.T01:CDS 60.0%
GGCGCAGCTTCTTCTCTCCC+TGG + Chr4:17750329-17750348 MsG0480019285.01.T01:CDS 65.0%
TTGCAAGCAGCCTGGCGGTC+AGG - Chr4:17750266-17750285 None:intergenic 65.0%
AGCAGCCTGGCGGTCAGGCG+TGG - Chr4:17750261-17750280 None:intergenic 75.0%
AGCCTGGCGGTCAGGCGTGG+TGG - Chr4:17750258-17750277 None:intergenic 75.0%
CACCACCACGCCTGACCGCC+AGG + Chr4:17750253-17750272 MsG0480019285.01.T01:CDS 75.0%
CTGGCGGTCAGGCGTGGTGG+TGG - Chr4:17750255-17750274 None:intergenic 75.0%
!! GCGGTCAGGCGTGGTGGTGG+CGG - Chr4:17750252-17750271 None:intergenic 75.0%
!! GTCAGGCGTGGTGGTGGCGG+CGG - Chr4:17750249-17750268 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 17750068 17750448 17750068 ID=MsG0480019285.01;Name=MsG0480019285.01
Chr4 mRNA 17750068 17750448 17750068 ID=MsG0480019285.01.T01;Parent=MsG0480019285.01;Name=MsG0480019285.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|126
Chr4 exon 17750068 17750448 17750068 ID=MsG0480019285.01.T01:exon:4619;Parent=MsG0480019285.01.T01
Chr4 CDS 17750068 17750448 17750068 ID=MsG0480019285.01.T01:cds;Parent=MsG0480019285.01.T01
Gene Sequence

>MsG0480019285.01.T01

ATGGCAAAAATGAAGGTAGCATGTGTTGTTTTGATGATGTGCATGATTGTTGCACCTATGGCAGATGCTGCAATCTCATGTGGACAAGTGAGTGGTGCTCTTTCTCCATGCATTGGATATCTTAGAGGTGGTGCTGGTCCATCTCCAGCATGCTGTGGTGGAGTGAAGAGACTTAACGCCGCCGCCACCACCACGCCTGACCGCCAGGCTGCTTGCAACTGCTTGAAAAGTGCTGCTGGTGCTATTTCTGGTTTGAATACAGGCGCAGCTTCTTCTCTCCCTGGCAAATGTGGTGTTAACATTCCTTACAAGATCAGTACCTCCACCAACTGTGCTACGTATGTTCATCTATTTTTCACTTCATTTTCAATCTTTAATTAA

Protein sequence

>MsG0480019285.01.T01

MAKMKVACVVLMMCMIVAPMADAAISCGQVSGALSPCIGYLRGGAGPSPACCGGVKRLNAAATTTPDRQAACNCLKSAAGAISGLNTGAASSLPGKCGVNIPYKISTSTNCATYVHLFFTSFSIFN*