AlfalfaGEDB | MsG0480023264.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023264.01

KEGG: -
GO: GO:0008152 GO:0016787
PFAM: PF13419

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023264.01.T01	QCD84683.1	60.708	565	143	12	1	545	1	506	0	604

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023264.01.T01	Q680K2	71.698	265	60	1	295	544	51	315	1.42E-136	401

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023264.01.T01	A0A4D6L849	60.708	565	143	12	1	545	1	506	0.0	604

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0480023264.01	-0.825261	5.161618e-54	4.681233e-51
MsG0080047910.01	MsG0480023264.01	0.818683	1.724689e-52	1.299705e-49
MsG0080047945.01	MsG0480023264.01	0.818886	1.551312e-52	1.175722e-49
MsG0080047956.01	MsG0480023264.01	0.800653	1.302601e-48	6.098617e-46
MsG0080048059.01	MsG0480023264.01	0.883539	4.118143e-71	2.622012e-67
MsG0080048256.01	MsG0480023264.01	-0.845735	3.372977e-59	5.675960e-56
MsG0080048528.01	MsG0480023264.01	0.817635	2.977066e-52	2.179741e-49
MsG0080048560.01	MsG0480023264.01	0.805245	1.464865e-49	7.711483e-47
MsG0080048595.01	MsG0480023264.01	-0.817031	4.072344e-52	2.932895e-49
MsG0080048597.01	MsG0480023264.01	-0.831278	1.827503e-55	1.973681e-52

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023264.01.T01	MTR_8g090025	92.015	263	4	2	300	545	56	318	4.40e-173	492
MsG0480023264.01.T01	MTR_8g090025	91.498	247	4	2	300	529	56	302	1.66e-161	462
MsG0480023264.01.T01	MTR_8g090020	78.694	291	47	5	1	288	1	279	2.82e-155	445
MsG0480023264.01.T01	MTR_1g060280	61.078	334	82	10	1	292	1	328	8.97e-119	354
MsG0480023264.01.T01	MTR_8g063930	42.319	345	147	9	1	309	1	329	2.04e-71	234
MsG0480023264.01.T01	MTR_3g075230	40.000	230	107	6	310	523	83	297	1.21e-43	158
MsG0480023264.01.T01	MTR_0937s0020	30.041	243	138	4	301	528	523	748	2.76e-24	107

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023264.01.T01	AT4G39970	71.698	265	60	1	295	544	51	315	1.45e-137	401
MsG0480023264.01.T01	AT3G48420	39.676	247	118	6	293	523	62	293	3.16e-46	165
MsG0480023264.01.T01	AT1G23840	25.698	358	170	11	10	294	4	338	7.99e-19	88.2
MsG0480023264.01.T01	AT1G23850	22.715	361	190	8	9	291	1	350	3.47e-18	86.7
MsG0480023264.01.T01	AT1G56500	28.755	233	140	8	309	534	76	289	1.49e-14	77.4
MsG0480023264.01.T01	AT1G56500	28.755	233	140	8	309	534	76	289	3.32e-14	76.3

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Find 104 sgRNAs with CRISPR-Local

Find 435 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGAAAGGGAGAGAAGTG+AGG	0.524585	4:-82875643	None:intergenic
AAACAATTGCTCAGAGTCCA+TGG	0.499769	4:-82876058	None:intergenic
AAAGTTGATGATGAGGAAAT+GGG	0.468831	4:+82876459	MsG0480023264.01.T01:CDS
AAAGTTTGAGGTTTATCACA+AGG	0.626874	4:+82883326	MsG0480023264.01.T01:three_prime_UTR
AAATAATCGTACAGCATCTC+TGG	0.438342	4:-82881429	None:intergenic
AAATAATCTTTGCTAAAACT+TGG	0.372913	4:-82875803	None:intergenic
AACGAAGTTGATTTCTTTCA+TGG	0.214823	4:-82875742	None:intergenic
AACTCCTCCTACTAGTGATG+AGG	0.626635	4:+82879519	MsG0480023264.01.T01:CDS
AAGCAGTCACATATATTGAC+GGG	0.575556	4:-82881930	None:intergenic
AATAATCTTTGCTAAAACTT+GGG	0.458252	4:-82875802	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTTAAGACTTAAATGGTG+GGG	-	Chr4:82882762-82882781	None:intergenic	30.0%
AAGCAATACCATTAAGTAGA+AGG	-	Chr4:82876869-82876888	None:intergenic	30.0%
ACAAGTGTTTGAACTTTCAT+AGG	-	Chr4:82881109-82881128	None:intergenic	30.0%
ACCAAACCAATCAAGTATAA+CGG	-	Chr4:82881526-82881545	None:intergenic	30.0%
ACTTAGATTGGAAATTATGC+AGG	+	Chr4:82879818-82879837	MsG0480023264.01.T01:intron	30.0%
ACTTTGAATGCTATAAAGGA+GGG	+	Chr4:82876331-82876350	MsG0480023264.01.T01:intron	30.0%
AGAGTATGGTGTAATTTCAT+TGG	+	Chr4:82881205-82881224	MsG0480023264.01.T01:intron	30.0%
AGATGAAACAACAAATACTG+AGG	+	Chr4:82882507-82882526	MsG0480023264.01.T01:intron	30.0%
AGATGGAGCTACATAATATT+TGG	+	Chr4:82876091-82876110	MsG0480023264.01.T01:CDS	30.0%
ATAACTGGATAAAGAGGTTT+AGG	+	Chr4:82878211-82878230	MsG0480023264.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	82875527	82883529	82875527	ID=MsG0480023264.01;Name=MsG0480023264.01
Chr4	mRNA	82875527	82883529	82875527	ID=MsG0480023264.01.T01;Parent=MsG0480023264.01;Name=MsG0480023264.01.T01;_AED=0.36;_eAED=0.36;_QI=0\|0.72\|0.75\|1\|1\|1\|12\|278\|545
Chr4	exon	82875527	82876256	82875527	ID=MsG0480023264.01.T01:exon:5384;Parent=MsG0480023264.01.T01
Chr4	exon	82876349	82876501	82876349	ID=MsG0480023264.01.T01:exon:5385;Parent=MsG0480023264.01.T01
Chr4	exon	82879127	82879355	82879127	ID=MsG0480023264.01.T01:exon:5386;Parent=MsG0480023264.01.T01
Chr4	exon	82879477	82879570	82879477	ID=MsG0480023264.01.T01:exon:5387;Parent=MsG0480023264.01.T01
Chr4	exon	82881389	82881439	82881389	ID=MsG0480023264.01.T01:exon:5388;Parent=MsG0480023264.01.T01
Chr4	exon	82881583	82881657	82881583	ID=MsG0480023264.01.T01:exon:5389;Parent=MsG0480023264.01.T01
Chr4	exon	82881748	82881781	82881748	ID=MsG0480023264.01.T01:exon:5390;Parent=MsG0480023264.01.T01
Chr4	exon	82881902	82881957	82881902	ID=MsG0480023264.01.T01:exon:5391;Parent=MsG0480023264.01.T01
Chr4	exon	82882060	82882119	82882060	ID=MsG0480023264.01.T01:exon:5392;Parent=MsG0480023264.01.T01
Chr4	exon	82882894	82882953	82882894	ID=MsG0480023264.01.T01:exon:5393;Parent=MsG0480023264.01.T01
Chr4	exon	82883067	82883113	82883067	ID=MsG0480023264.01.T01:exon:5394;Parent=MsG0480023264.01.T01
Chr4	exon	82883203	82883529	82883203	ID=MsG0480023264.01.T01:exon:5395;Parent=MsG0480023264.01.T01
Chr4	CDS	82875527	82876256	82875527	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82876349	82876501	82876349	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82879127	82879355	82879127	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82879477	82879570	82879477	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82881389	82881439	82881389	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82881583	82881657	82881583	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82881748	82881781	82881748	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82881902	82881957	82881902	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82882060	82882119	82882060	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82882894	82882953	82882894	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82883067	82883113	82883067	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	CDS	82883203	82883251	82883203	ID=MsG0480023264.01.T01:cds;Parent=MsG0480023264.01.T01
Chr4	three_prime_UTR	82883252	82883529	82883252	ID=MsG0480023264.01.T01:three_prime_utr;Parent=MsG0480023264.01.T01

Gene Sequence

>MsG0480023264.01.T01

ATGGAGAGTGAAACCAAACATATTATTAAGCAGAAGAAGAACATTGATGTCTTAGATATCCTAGGTGAAACTCTAACCATCTATTTCAAAAACATAAACTTCATCATCTTCACTTTCCTCACTTCTCTCCCTTTCTTTTTTCTCATGGTTTACTTTGAAACATTGTTCCAACAAACTCTTGTTCAAACTCCAAAAATCATCTTATCTTTACCTTTCCATGAAAGAAATCAACTTCGTTTCTATTCAGTTATCGACCACAATTTCATTTATAATGGCCCAAGTTTTAGCAAAGATTATTTACCTCTTTTGATTCAACTTGGATTTATTTACACTATCCCTCTTCATTTCCTAGAATTCTGTTCTAAAATATTAACAATGGATTTAGCTTCAAAGCTTATCAATTCAGAAGAAAACCTTAAAATGAGTCTCAAACACATGTTTCAAAATTCAATTGGCGTGTCAACCATGAAAGGAACATTTGTGACTTCTTTGTATACACTTGCATTATCAAATTGTCTTCTTATTGCATTTCCATGGACTCTGAGCAATTGTTTCATTTTTACTAGATGGAGCTACATAATATTTGGAACAATTTGTTTTGTAGCTATTGGGAAATTGATGATGGTGTATTTGGAATGGAGTGCTATTTGGAATATGAGTATAGTGATATCAGTGTTGGAGGGTATTTATGGAATCGGAGCTATGAGAGTTTCATATTACTTCAGCAGTGGAGGGAGTGGAATTTTTATACAGATTGGGATTCTTAGTGTGGTGAATACACTGAAATGGGTGTCTTGTGTGATTTATTTCAATGATTGTAAGGAGAGGAAAATGGAGAAGAAAGTTGATGATGAGGAAATGGGCAAGGATAAGCTTGTAACTATCTCAGCTTCATCTTCATCTTCATCTTCATCTTCGTTTCAAGCTCTTATATTTGACTGTGACGGCGTCATCTTGGAATCTGAGCACTTACATCGTCAGGCTTATAATGATGCATTTCTTCACTTCAATGTTCGTTCTCCATCTTCTTCTCCTCAACCACTCAACTGGGATATAGAATTCTATGATCAACTTCAGAACCAAATTGGTGGTGGCAAACCCAAAATGCGATGGTACTTTAAGGAACATGGGTGGCCTTCATCAACGTTATTCGAAACTCCTCCTACTAGTGATGAGGAACGAGCCAAGTTGATTGACACTCTTCAGGTAAAACCTAGACCAGGAGTTTTGAAATTGATGGATGAAGCCAGAGATGCTGGGAAAAAGCTAGCTGTTTGCTCTGCTGCAACTAAAAGTTCAGTTATTCTTTGTCTTGAAAACCTTATTGGAATTGAGCGCTTTCAAAGCCTTGATTGCTTCCTTGCTGGTGATGATGTGAAGGAAAAGAAGCCCGACCCGTCAATATATGTGACTGCTTCCCAGAAGTTGGGTGTATCAGAGAAAAATTGCTTGGTGGTAGAGGATAGTGTTATTGGTTTACAGGCAGCAACACAAGCAGGGATGTCTTGTGTGGTTACCTACACATCTTCCACTGCTGAGCAGGATTTCAAAGAAGCCATAGCAATCTACCCTGACTTGAGTAACGTAAGCTTGAAAGATTTGGAGTTGCTACTTCAAGATGTTGTAGCTGCTAAATAG

Protein sequence

>MsG0480023264.01.T01

MESETKHIIKQKKNIDVLDILGETLTIYFKNINFIIFTFLTSLPFFFLMVYFETLFQQTLVQTPKIILSLPFHERNQLRFYSVIDHNFIYNGPSFSKDYLPLLIQLGFIYTIPLHFLEFCSKILTMDLASKLINSEENLKMSLKHMFQNSIGVSTMKGTFVTSLYTLALSNCLLIAFPWTLSNCFIFTRWSYIIFGTICFVAIGKLMMVYLEWSAIWNMSIVISVLEGIYGIGAMRVSYYFSSGGSGIFIQIGILSVVNTLKWVSCVIYFNDCKERKMEKKVDDEEMGKDKLVTISASSSSSSSSSFQALIFDCDGVILESEHLHRQAYNDAFLHFNVRSPSSSPQPLNWDIEFYDQLQNQIGGGKPKMRWYFKEHGWPSSTLFETPPTSDEERAKLIDTLQVKPRPGVLKLMDEARDAGKKLAVCSAATKSSVILCLENLIGIERFQSLDCFLAGDDVKEKKPDPSIYVTASQKLGVSEKNCLVVEDSVIGLQAATQAGMSCVVTYTSSTAEQDFKEAIAIYPDLSNVSLKDLELLLQDVVAAK*