AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023300.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023300.01.T01 MTR_8g090055 83.284 335 21 4 1 335 1 300 0.0 530
MsG0480023300.01.T01 MTR_5g037310 38.489 278 139 8 5 267 6 266 1.78e-52 175
MsG0480023300.01.T01 MTR_5g037310 38.489 278 139 8 5 267 6 266 2.14e-52 175
MsG0480023300.01.T01 MTR_4g105550 29.286 280 157 12 13 267 12 275 5.30e-25 103
MsG0480023300.01.T01 MTR_4g123700 28.514 249 153 8 31 268 30 264 7.83e-23 97.1
MsG0480023300.01.T01 MTR_4g132450 28.358 268 146 11 33 274 36 283 3.38e-22 95.9
MsG0480023300.01.T01 MTR_2g023970 27.737 274 152 12 27 274 16 269 9.70e-20 88.6
MsG0480023300.01.T01 MTR_3g069050 27.843 255 142 14 39 282 31 254 7.41e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023300.01.T01 AT1G10380 40.964 249 126 6 34 267 28 270 2.56e-45 157
MsG0480023300.01.T01 AT1G10380 40.964 249 126 6 34 267 28 270 6.45e-45 155
MsG0480023300.01.T01 AT3G17350 29.670 273 161 10 16 270 12 271 8.82e-23 96.3
MsG0480023300.01.T01 AT1G11915 29.964 277 159 12 7 260 1 265 9.96e-22 94.4
MsG0480023300.01.T01 AT3G17350 29.545 264 156 9 16 262 12 262 1.90e-21 93.2
MsG0480023300.01.T01 AT5G50290 28.159 277 160 12 17 267 3 266 1.64e-20 90.5

Find 114 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTACTCATTGGGTTTA+TGG 0.115730 4:+83515838 MsG0480023300.01.T01:CDS
TGAAAGTGGATGAAGATATT+TGG 0.257191 4:-83515579 None:intergenic
TTGGCACAGGTCCTGGTTGC+GGG 0.266168 4:+83515356 MsG0480023300.01.T01:CDS
GTGTATGAAATGGAGGTTAT+AGG 0.283646 4:-83515463 None:intergenic
TGCGTCAAAGCTTCCACTTT+TGG 0.298303 4:+83516929 MsG0480023300.01.T01:three_prime_UTR
GGCAGTCAGTGCACAAAGTT+CGG 0.327170 4:+83516792 MsG0480023300.01.T01:three_prime_UTR
CTCACAACTAGTACATTTAG+TGG 0.331784 4:-83515905 None:intergenic
ATCAAAACCAAGATTTCCTT+TGG 0.338876 4:+83516025 MsG0480023300.01.T01:CDS
GTTCGGGCATACGCAGGGTC+TGG 0.362335 4:+83516809 MsG0480023300.01.T01:three_prime_UTR
TTTGATGTGCATGTTATGTA+TGG 0.363083 4:-83515394 None:intergenic
GTTAAATCAATGCCAAAGTT+TGG 0.363622 4:-83515547 None:intergenic
TACATAACATGCACATCAAA+TGG 0.388250 4:+83515397 MsG0480023300.01.T01:CDS
GCAGTCAGTGCACAAAGTTC+GGG 0.389796 4:+83516793 MsG0480023300.01.T01:three_prime_UTR
CGGGGAACCTAGACGGCAAA+GGG 0.391013 4:+83515746 MsG0480023300.01.T01:CDS
TTTGGCACAGGTCCTGGTTG+CGG 0.395015 4:+83515355 MsG0480023300.01.T01:CDS
ACACAAACAAATCCACTTCC+TGG 0.398611 4:-83515958 None:intergenic
TCAACTCCCCTTTGCCGTCT+AGG 0.402495 4:-83515753 None:intergenic
TAGAGTCCCAAAGGAAATCT+TGG 0.407259 4:-83516032 None:intergenic
TATCCCTTTGGCACAGGTCC+TGG 0.410285 4:+83515349 MsG0480023300.01.T01:CDS
GATCGTAATTAGAGCAATCA+AGG 0.418096 4:-83516005 None:intergenic
CCCTACTGATCCTACTCATT+GGG 0.420444 4:+83515831 MsG0480023300.01.T01:CDS
GTTATGTATGGGTTGAATAG+TGG 0.421768 4:-83515382 None:intergenic
AAACAATATGCCAAAGTAAT+GGG 0.422653 4:+83516358 MsG0480023300.01.T01:CDS
TGTATGAAATGGAGGTTATA+GGG 0.425846 4:-83515462 None:intergenic
TGTTGTTGAGGCTGTGGGTG+TGG 0.432596 4:-83515284 None:intergenic
ATAATAGTTACCCATTACTT+TGG 0.439184 4:-83516368 None:intergenic
TATACACAACATGTGTTAGT+AGG 0.444066 4:-83515718 None:intergenic
TATGAACAGTTGGGAATGTC+TGG 0.453180 4:+83516502 MsG0480023300.01.T01:intron
ACCCTACTGATCCTACTCAT+TGG 0.456155 4:+83515830 MsG0480023300.01.T01:CDS
TTCGGGCATACGCAGGGTCT+GGG 0.458701 4:+83516810 MsG0480023300.01.T01:three_prime_UTR
TTTGTTTGTGTTTGTAAAGG+TGG 0.459550 4:+83515970 MsG0480023300.01.T01:CDS
AAAACAATATGCCAAAGTAA+TGG 0.463455 4:+83516357 MsG0480023300.01.T01:CDS
TTTGTGTACAATACATCAAA+TGG 0.464239 4:-83516844 None:intergenic
CTGGAGTTCCGCATCTTGGC+TGG 0.464598 4:+83516521 MsG0480023300.01.T01:CDS
AATGGAGGTTATAGGGTATG+AGG 0.466527 4:-83515455 None:intergenic
TCAAAACCAAGATTTCCTTT+GGG 0.473584 4:+83516026 MsG0480023300.01.T01:CDS
AGATAACAATACACCAAAAG+TGG 0.476574 4:-83516942 None:intergenic
TGTTAGTGTTGTTGAGGCTG+TGG 0.477766 4:-83515290 None:intergenic
ATGTCTGGAGTTCCGCATCT+TGG 0.489153 4:+83516517 MsG0480023300.01.T01:CDS
ATGCAAACGTCACCAAACTT+TGG 0.492190 4:+83515535 MsG0480023300.01.T01:CDS
ATACAAGTCAAGTATCCCTT+TGG 0.494214 4:+83515337 MsG0480023300.01.T01:CDS
CCGGGGAACCTAGACGGCAA+AGG 0.496288 4:+83515745 MsG0480023300.01.T01:CDS
CACATGTTGTGTATACTCAC+CGG 0.497181 4:+83515726 MsG0480023300.01.T01:CDS
ATCAAAAGAAGTGTCACAAA+TGG 0.498238 4:-83515632 None:intergenic
CCTTTGCCGTCTAGGTTCCC+CGG 0.498477 4:-83515745 None:intergenic
TATTCAACATCAATATGTAA+TGG 0.500190 4:-83516999 None:intergenic
TGTGAGTGTGAGAGTGGAAG+TGG 0.506008 4:-83515485 None:intergenic
CTAAGATGAGAATCATGAAA+AGG 0.513752 4:-83515258 None:intergenic
GGGTGGTGTGAGTGTGAGAG+TGG 0.515614 4:-83515491 None:intergenic
TAATCGGATGATAATGCTGC+TGG 0.516924 4:+83516563 MsG0480023300.01.T01:exon
TACACTAGACTAGACTTCAT+AGG 0.517749 4:-83516716 None:intergenic
GTTAGTGTTGTTGAGGCTGT+GGG 0.518223 4:-83515289 None:intergenic
TATACTTCGGTTGTGTCACT+TGG 0.519729 4:+83515802 MsG0480023300.01.T01:CDS
AGTGGAAGTGGTGTATGAAA+TGG 0.521135 4:-83515473 None:intergenic
CACAAAGTTCGGGCATACGC+AGG 0.521934 4:+83516803 MsG0480023300.01.T01:three_prime_UTR
GGATATGATCCTCCAGGAAG+TGG 0.524771 4:+83515946 MsG0480023300.01.T01:CDS
AATAGTGGGGACCCGCAACC+AGG 0.526866 4:-83515367 None:intergenic
AGTAGTGGTGGTAGTGCCAT+TGG 0.534426 4:-83515211 None:intergenic
GGATTTGTTTGTGTTTGTAA+AGG 0.540887 4:+83515967 MsG0480023300.01.T01:CDS
GCAACCAGGACCTGTGCCAA+AGG 0.541377 4:-83515353 None:intergenic
AAGTGGAAGCTTTGACGCAA+AGG 0.544197 4:-83516925 None:intergenic
GCAAGAGAGGAGGATGAAAG+TGG 0.545372 4:-83515593 None:intergenic
GTCAAGTATCCCTTTGGCAC+AGG 0.545996 4:+83515343 MsG0480023300.01.T01:CDS
GCATACGCAGGGTCTGGGAA+AGG 0.546643 4:+83516815 MsG0480023300.01.T01:three_prime_UTR
GTGGAAAGAGGTGGTAGTAG+TGG 0.547172 4:-83515226 None:intergenic
ACATGTTGTGTATACTCACC+GGG 0.550531 4:+83515727 MsG0480023300.01.T01:CDS
ACCCAATGAGTAGGATCAGT+AGG 0.552236 4:-83515832 None:intergenic
TGCCCTCAAGTAGTCTCACT+TGG 0.552797 4:+83515679 MsG0480023300.01.T01:CDS
GAGAGTAGTGGTGGTGTATG+TGG 0.555000 4:+83515925 MsG0480023300.01.T01:CDS
TACTCATTGGGTTTATGGAG+TGG 0.555908 4:+83515843 MsG0480023300.01.T01:CDS
CAACCAGGACCTGTGCCAAA+GGG 0.563262 4:-83515352 None:intergenic
TTGATGTGCATGTTATGTAT+GGG 0.566236 4:-83515393 None:intergenic
TAGACTAGACTTCATAGGAA+TGG 0.567381 4:-83516711 None:intergenic
TTATGTATGGGTTGAATAGT+GGG 0.567840 4:-83515381 None:intergenic
AGGTTGATGAGCGTGGAAAG+AGG 0.567988 4:-83515238 None:intergenic
CATTATCATCCGATTACACT+AGG 0.569260 4:-83516556 None:intergenic
GGAAGTGGTGTATGAAATGG+AGG 0.572115 4:-83515470 None:intergenic
AAAGTTTGGTGACGTTTGCA+TGG 0.574117 4:-83515533 None:intergenic
CCCAATGAGTAGGATCAGTA+GGG 0.577746 4:-83515831 None:intergenic
CATGAAAAGGTTGATGAGCG+TGG 0.579609 4:-83515245 None:intergenic
GAGGAGAGATAGAGTCCCAA+AGG 0.583741 4:-83516041 None:intergenic
TGATTGTAGTGGTTATACTT+CGG 0.584725 4:+83515789 MsG0480023300.01.T01:CDS
GGTGGTGCAAGTAGACATGT+AGG 0.584778 4:-83515512 None:intergenic
GAAAGAGGTGGTAGTAGTGG+TGG 0.586256 4:-83515223 None:intergenic
GCACACTGTTAGTGTTGTTG+AGG 0.594642 4:-83515296 None:intergenic
TACCAAGTGAGACTACTTGA+GGG 0.596440 4:-83515681 None:intergenic
ATACCAAGTGAGACTACTTG+AGG 0.598463 4:-83515682 None:intergenic
CTTAGAGAGAATGATTGTAG+TGG 0.610496 4:+83515778 MsG0480023300.01.T01:CDS
GTGGATGAAGATATTTGGAA+TGG 0.613981 4:-83515574 None:intergenic
TACTCACCGGGGAACCTAGA+CGG 0.615280 4:+83515739 MsG0480023300.01.T01:CDS
AACATACTGTTGTACATGGG+AGG 0.621855 4:-83516063 None:intergenic
ACTCCATAAACCCAATGAGT+AGG 0.626290 4:-83515841 None:intergenic
CTAACAGTTAAGTCATTCAG+AGG 0.631884 4:+83516604 MsG0480023300.01.T01:three_prime_UTR
ATACTGTTGTACATGGGAGG+AGG 0.638228 4:-83516060 None:intergenic
TTGATGAGCGTGGAAAGAGG+TGG 0.643765 4:-83515235 None:intergenic
GTATGTGGATATGATCCTCC+AGG 0.645044 4:+83515940 MsG0480023300.01.T01:CDS
AAATGATACCAGCCAAGATG+CGG 0.645878 4:-83516529 None:intergenic
ACAAAGTTCGGGCATACGCA+GGG 0.650912 4:+83516804 MsG0480023300.01.T01:three_prime_UTR
AAGAGTAAAGAGAAGCACAA+AGG 0.652408 4:-83515657 None:intergenic
TGGAACACAAGCAAAGCCAA+TGG 0.663304 4:+83515195 None:intergenic
GTTTGGTGACGTTTGCATGG+TGG 0.670403 4:-83515530 None:intergenic
GTGGCATTGAAATATACTCA+TGG 0.674830 4:+83515862 MsG0480023300.01.T01:CDS
GTATTGAGCCACATGTGTCA+TGG 0.676021 4:-83515318 None:intergenic
AATCGGATGATAATGCTGCT+GGG 0.679814 4:+83516564 MsG0480023300.01.T01:exon
ACAGTTAAGTCATTCAGAGG+AGG 0.686509 4:+83516607 MsG0480023300.01.T01:three_prime_UTR
GGGGAACCTAGACGGCAAAG+GGG 0.692978 4:+83515747 MsG0480023300.01.T01:CDS
GTGGTGCAAGTAGACATGTA+GGG 0.696710 4:-83515511 None:intergenic
ACTAGTTGTGAGAGTAGTGG+TGG 0.700797 4:+83515916 MsG0480023300.01.T01:CDS
TGTACTAGTTGTGAGAGTAG+TGG 0.707622 4:+83515913 MsG0480023300.01.T01:CDS
TATGTATGGGTTGAATAGTG+GGG 0.729462 4:-83515380 None:intergenic
GTGCAAGTAGACATGTAGGG+TGG 0.743146 4:-83515508 None:intergenic
CATGTTGTGTATACTCACCG+GGG 0.745636 4:+83515728 MsG0480023300.01.T01:CDS
ACAGTGTGCCATGACACATG+TGG 0.759996 4:+83515310 MsG0480023300.01.T01:CDS
CAAACAAATCCACTTCCTGG+AGG 0.782247 4:-83515955 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATAAGTAAGTAATTA+GGG + Chr4:83516196-83516215 MsG0480023300.01.T01:intron 10.0%
!! TTATATATAAGTAAGTAATT+AGG + Chr4:83516195-83516214 MsG0480023300.01.T01:intron 10.0%
!! AACAATTAAAACTATCTTTA+GGG + Chr4:83517021-83517040 MsG0480023300.01.T01:three_prime_UTR 15.0%
!! ATATATAAGTAAGTAATTAG+GGG + Chr4:83516197-83516216 MsG0480023300.01.T01:intron 15.0%
!! GTATTGTCAAAAAAATAAAA+GGG + Chr4:83516771-83516790 MsG0480023300.01.T01:three_prime_UTR 15.0%
!! TAACAATTAAAACTATCTTT+AGG + Chr4:83517020-83517039 MsG0480023300.01.T01:three_prime_UTR 15.0%
!! TGTATTGTCAAAAAAATAAA+AGG + Chr4:83516770-83516789 MsG0480023300.01.T01:three_prime_UTR 15.0%
!! AAAGTACATTCAAAGAAAAA+TGG + Chr4:83516670-83516689 MsG0480023300.01.T01:three_prime_UTR 20.0%
!! TATTATTCTATACACTTGAA+AGG + Chr4:83516385-83516404 MsG0480023300.01.T01:intron 20.0%
!! TATTCAACATCAATATGTAA+TGG - Chr4:83517002-83517021 None:intergenic 20.0%
!!! GTCTTTTTTAATTTTCATAC+AGG + Chr4:83516163-83516182 MsG0480023300.01.T01:intron 20.0%
!!! TTTTTTTTTTTGTACAAGTC+TGG + Chr4:83516747-83516766 MsG0480023300.01.T01:three_prime_UTR 20.0%
! AAAAACATACTGTTGTACAT+GGG - Chr4:83516069-83516088 None:intergenic 25.0%
! AAAAAGGAAAAATAGATGGT+TGG + Chr4:83516292-83516311 MsG0480023300.01.T01:CDS 25.0%
! AAAACAATATGCCAAAGTAA+TGG + Chr4:83516357-83516376 MsG0480023300.01.T01:CDS 25.0%
! AAACAATATGCCAAAGTAAT+GGG + Chr4:83516358-83516377 MsG0480023300.01.T01:CDS 25.0%
! AAAGAAACAACAAAAAGAGA+AGG - Chr4:83516092-83516111 None:intergenic 25.0%
! ATAATAGTTACCCATTACTT+TGG - Chr4:83516371-83516390 None:intergenic 25.0%
! CAAGAAAAAGGAAAAATAGA+TGG + Chr4:83516288-83516307 MsG0480023300.01.T01:intron 25.0%
! CTCTGTAAAAAAAAATTGCA+TGG + Chr4:83516963-83516982 MsG0480023300.01.T01:three_prime_UTR 25.0%
! TTTGTGTACAATACATCAAA+TGG - Chr4:83516847-83516866 None:intergenic 25.0%
!! ATTTTCTGAGTACAAGAAAA+AGG + Chr4:83516276-83516295 MsG0480023300.01.T01:intron 25.0%
!! TAACTTTTGTGCATTAAAGA+AGG - Chr4:83516643-83516662 None:intergenic 25.0%
!! TCTTTGAATGTACTTTTCTA+AGG - Chr4:83516666-83516685 None:intergenic 25.0%
!!! AAATGGTTGAACTTTTTTCT+TGG - Chr4:83515618-83515637 None:intergenic 25.0%
!!! CACAAATTTACCTTGTTTTT+AGG + Chr4:83516861-83516880 MsG0480023300.01.T01:three_prime_UTR 25.0%
!!! TTTATATTGATTCTCTCTCT+TGG + Chr4:83516431-83516450 MsG0480023300.01.T01:intron 25.0%
AAAAAAGACCCGAAAACTAT+GGG - Chr4:83516152-83516171 None:intergenic 30.0%
AGATAACAATACACCAAAAG+TGG - Chr4:83516945-83516964 None:intergenic 30.0%
ATCAAAACCAAGATTTCCTT+TGG + Chr4:83516025-83516044 MsG0480023300.01.T01:CDS 30.0%
CTAAGATGAGAATCATGAAA+AGG - Chr4:83515261-83515280 None:intergenic 30.0%
GAAAAACATACTGTTGTACA+TGG - Chr4:83516070-83516089 None:intergenic 30.0%
GAAATGAGTCAAATGACTAT+TGG + Chr4:83516219-83516238 MsG0480023300.01.T01:intron 30.0%
GTTAAATCAATGCCAAAGTT+TGG - Chr4:83515550-83515569 None:intergenic 30.0%
TAAAAAAGACCCGAAAACTA+TGG - Chr4:83516153-83516172 None:intergenic 30.0%
TACATAACATGCACATCAAA+TGG + Chr4:83515397-83515416 MsG0480023300.01.T01:CDS 30.0%
TATACACAACATGTGTTAGT+AGG - Chr4:83515721-83515740 None:intergenic 30.0%
TCAAAACCAAGATTTCCTTT+GGG + Chr4:83516026-83516045 MsG0480023300.01.T01:CDS 30.0%
TGAAAGTGGATGAAGATATT+TGG - Chr4:83515582-83515601 None:intergenic 30.0%
TGATTGTAGTGGTTATACTT+CGG + Chr4:83515789-83515808 MsG0480023300.01.T01:CDS 30.0%
TGTATGAAATGGAGGTTATA+GGG - Chr4:83515465-83515484 None:intergenic 30.0%
TTGATGTGCATGTTATGTAT+GGG - Chr4:83515396-83515415 None:intergenic 30.0%
TTTGATGTGCATGTTATGTA+TGG - Chr4:83515397-83515416 None:intergenic 30.0%
TTTGTTTGTGTTTGTAAAGG+TGG + Chr4:83515970-83515989 MsG0480023300.01.T01:CDS 30.0%
! GGATTTGTTTGTGTTTGTAA+AGG + Chr4:83515967-83515986 MsG0480023300.01.T01:CDS 30.0%
! TTATGTATGGGTTGAATAGT+GGG - Chr4:83515384-83515403 None:intergenic 30.0%
! TTTACATTCCCCATAGTTTT+CGG + Chr4:83516140-83516159 MsG0480023300.01.T01:intron 30.0%
!! ATCAAAAGAAGTGTCACAAA+TGG - Chr4:83515635-83515654 None:intergenic 30.0%
AAAAAGACCCGAAAACTATG+GGG - Chr4:83516151-83516170 None:intergenic 35.0%
AAAAATAGATGGTTGGACGA+TGG + Chr4:83516299-83516318 MsG0480023300.01.T01:CDS 35.0%
ATACAAGTCAAGTATCCCTT+TGG + Chr4:83515337-83515356 MsG0480023300.01.T01:CDS 35.0%
CAGTCTCTTACCTAAAAACA+AGG - Chr4:83516874-83516893 None:intergenic 35.0%
CATTATCATCCGATTACACT+AGG - Chr4:83516559-83516578 None:intergenic 35.0%
CTAACAGTTAAGTCATTCAG+AGG + Chr4:83516604-83516623 MsG0480023300.01.T01:three_prime_UTR 35.0%
CTCACAACTAGTACATTTAG+TGG - Chr4:83515908-83515927 None:intergenic 35.0%
CTGTTCTGTTATGAACAGTT+GGG + Chr4:83516493-83516512 MsG0480023300.01.T01:intron 35.0%
CTTAGAGAGAATGATTGTAG+TGG + Chr4:83515778-83515797 MsG0480023300.01.T01:CDS 35.0%
GATCGTAATTAGAGCAATCA+AGG - Chr4:83516008-83516027 None:intergenic 35.0%
GTGGATGAAGATATTTGGAA+TGG - Chr4:83515577-83515596 None:intergenic 35.0%
GTGTATGAAATGGAGGTTAT+AGG - Chr4:83515466-83515485 None:intergenic 35.0%
GTGTTAGTAGGAGAAAAAAG+AGG - Chr4:83515709-83515728 None:intergenic 35.0%
TACACTAGACTAGACTTCAT+AGG - Chr4:83516719-83516738 None:intergenic 35.0%
TAGACTAGACTTCATAGGAA+TGG - Chr4:83516714-83516733 None:intergenic 35.0%
! AAGAGTAAAGAGAAGCACAA+AGG - Chr4:83515660-83515679 None:intergenic 35.0%
! CATTTTCAGCCTAGTGTAAT+CGG + Chr4:83516547-83516566 MsG0480023300.01.T01:exon 35.0%
! GTGGCATTGAAATATACTCA+TGG + Chr4:83515862-83515881 MsG0480023300.01.T01:CDS 35.0%
! GTTATGTATGGGTTGAATAG+TGG - Chr4:83515385-83515404 None:intergenic 35.0%
! TATGTATGGGTTGAATAGTG+GGG - Chr4:83515383-83515402 None:intergenic 35.0%
! TTACATTCCCCATAGTTTTC+GGG + Chr4:83516141-83516160 MsG0480023300.01.T01:intron 35.0%
!!! AACTTTTTTCTTGGCAAGAG+AGG - Chr4:83515609-83515628 None:intergenic 35.0%
AAATGATACCAGCCAAGATG+CGG - Chr4:83516532-83516551 None:intergenic 40.0%
AACATACTGTTGTACATGGG+AGG - Chr4:83516066-83516085 None:intergenic 40.0%
AATGGAGGTTATAGGGTATG+AGG - Chr4:83515458-83515477 None:intergenic 40.0%
ACACAAACAAATCCACTTCC+TGG - Chr4:83515961-83515980 None:intergenic 40.0%
ACAGTTAAGTCATTCAGAGG+AGG + Chr4:83516607-83516626 MsG0480023300.01.T01:three_prime_UTR 40.0%
ACATGTTGTGTATACTCACC+GGG + Chr4:83515727-83515746 MsG0480023300.01.T01:CDS 40.0%
ACTCCATAAACCCAATGAGT+AGG - Chr4:83515844-83515863 None:intergenic 40.0%
AGTGGAAGTGGTGTATGAAA+TGG - Chr4:83515476-83515495 None:intergenic 40.0%
ATACCAAGTGAGACTACTTG+AGG - Chr4:83515685-83515704 None:intergenic 40.0%
ATGCAAACGTCACCAAACTT+TGG + Chr4:83515535-83515554 MsG0480023300.01.T01:CDS 40.0%
CACATGTTGTGTATACTCAC+CGG + Chr4:83515726-83515745 MsG0480023300.01.T01:CDS 40.0%
CCAACTGTTCATAACAGAAC+AGG - Chr4:83516495-83516514 None:intergenic 40.0%
CCTGTTCTGTTATGAACAGT+TGG + Chr4:83516492-83516511 MsG0480023300.01.T01:intron 40.0%
GATCCTACTCATTGGGTTTA+TGG + Chr4:83515838-83515857 MsG0480023300.01.T01:CDS 40.0%
TACCAAGTGAGACTACTTGA+GGG - Chr4:83515684-83515703 None:intergenic 40.0%
TACTCATTGGGTTTATGGAG+TGG + Chr4:83515843-83515862 MsG0480023300.01.T01:CDS 40.0%
TAGAGTCCCAAAGGAAATCT+TGG - Chr4:83516035-83516054 None:intergenic 40.0%
TATGAACAGTTGGGAATGTC+TGG + Chr4:83516502-83516521 MsG0480023300.01.T01:intron 40.0%
TGTACTAGTTGTGAGAGTAG+TGG + Chr4:83515913-83515932 MsG0480023300.01.T01:CDS 40.0%
! AATCGGATGATAATGCTGCT+GGG + Chr4:83516564-83516583 MsG0480023300.01.T01:exon 40.0%
! ATGAGGATGTGTGAGTTTTG+AGG - Chr4:83515441-83515460 None:intergenic 40.0%
! TAATCGGATGATAATGCTGC+TGG + Chr4:83516563-83516582 MsG0480023300.01.T01:exon 40.0%
! TATACTTCGGTTGTGTCACT+TGG + Chr4:83515802-83515821 MsG0480023300.01.T01:CDS 40.0%
!! AAAGTTTGGTGACGTTTGCA+TGG - Chr4:83515536-83515555 None:intergenic 40.0%
!!! TGTGAGTTTTGAGGATGAGT+TGG - Chr4:83515432-83515451 None:intergenic 40.0%
!!! TTTTTTCTTGGCAAGAGAGG+AGG - Chr4:83515606-83515625 None:intergenic 40.0%
ACCCAATGAGTAGGATCAGT+AGG - Chr4:83515835-83515854 None:intergenic 45.0%
ACCCTACTGATCCTACTCAT+TGG + Chr4:83515830-83515849 MsG0480023300.01.T01:CDS 45.0%
ACTAGTTGTGAGAGTAGTGG+TGG + Chr4:83515916-83515935 MsG0480023300.01.T01:CDS 45.0%
ATACTGTTGTACATGGGAGG+AGG - Chr4:83516063-83516082 None:intergenic 45.0%
CAAACAAATCCACTTCCTGG+AGG - Chr4:83515958-83515977 None:intergenic 45.0%
CATGTTGTGTATACTCACCG+GGG + Chr4:83515728-83515747 MsG0480023300.01.T01:CDS 45.0%
CCCAATGAGTAGGATCAGTA+GGG - Chr4:83515834-83515853 None:intergenic 45.0%
CCCTACTGATCCTACTCATT+GGG + Chr4:83515831-83515850 MsG0480023300.01.T01:CDS 45.0%
GGAAGTGGTGTATGAAATGG+AGG - Chr4:83515473-83515492 None:intergenic 45.0%
GTATGTGGATATGATCCTCC+AGG + Chr4:83515940-83515959 MsG0480023300.01.T01:CDS 45.0%
GTATTGAGCCACATGTGTCA+TGG - Chr4:83515321-83515340 None:intergenic 45.0%
GTGGTGCAAGTAGACATGTA+GGG - Chr4:83515514-83515533 None:intergenic 45.0%
! AAGTGGAAGCTTTGACGCAA+AGG - Chr4:83516928-83516947 None:intergenic 45.0%
! GCACACTGTTAGTGTTGTTG+AGG - Chr4:83515299-83515318 None:intergenic 45.0%
! GTTAGTGTTGTTGAGGCTGT+GGG - Chr4:83515292-83515311 None:intergenic 45.0%
! TGCGTCAAAGCTTCCACTTT+TGG + Chr4:83516929-83516948 MsG0480023300.01.T01:three_prime_UTR 45.0%
! TGTTAGTGTTGTTGAGGCTG+TGG - Chr4:83515293-83515312 None:intergenic 45.0%
!! CATGAAAAGGTTGATGAGCG+TGG - Chr4:83515248-83515267 None:intergenic 45.0%
ACAAAGTTCGGGCATACGCA+GGG + Chr4:83516804-83516823 MsG0480023300.01.T01:three_prime_UTR 50.0%
ACAGTGTGCCATGACACATG+TGG + Chr4:83515310-83515329 MsG0480023300.01.T01:CDS 50.0%
GAAAGAGGTGGTAGTAGTGG+TGG - Chr4:83515226-83515245 None:intergenic 50.0%
GAGGAGAGATAGAGTCCCAA+AGG - Chr4:83516044-83516063 None:intergenic 50.0%
GCAAGAGAGGAGGATGAAAG+TGG - Chr4:83515596-83515615 None:intergenic 50.0%
GCAGTCAGTGCACAAAGTTC+GGG + Chr4:83516793-83516812 MsG0480023300.01.T01:three_prime_UTR 50.0%
GGATATGATCCTCCAGGAAG+TGG + Chr4:83515946-83515965 MsG0480023300.01.T01:CDS 50.0%
GGCAGTCAGTGCACAAAGTT+CGG + Chr4:83516792-83516811 MsG0480023300.01.T01:three_prime_UTR 50.0%
GGTGGTGCAAGTAGACATGT+AGG - Chr4:83515515-83515534 None:intergenic 50.0%
GTCAAGTATCCCTTTGGCAC+AGG + Chr4:83515343-83515362 MsG0480023300.01.T01:CDS 50.0%
GTGCAAGTAGACATGTAGGG+TGG - Chr4:83515511-83515530 None:intergenic 50.0%
GTGGAAAGAGGTGGTAGTAG+TGG - Chr4:83515229-83515248 None:intergenic 50.0%
TGCCCTCAAGTAGTCTCACT+TGG + Chr4:83515679-83515698 MsG0480023300.01.T01:CDS 50.0%
TGTGAGTGTGAGAGTGGAAG+TGG - Chr4:83515488-83515507 None:intergenic 50.0%
TTGATGAGCGTGGAAAGAGG+TGG - Chr4:83515238-83515257 None:intergenic 50.0%
! AGTAGTGGTGGTAGTGCCAT+TGG - Chr4:83515214-83515233 None:intergenic 50.0%
! ATGTCTGGAGTTCCGCATCT+TGG + Chr4:83516517-83516536 MsG0480023300.01.T01:CDS 50.0%
! GAGAGTAGTGGTGGTGTATG+TGG + Chr4:83515925-83515944 MsG0480023300.01.T01:CDS 50.0%
!! AGGTTGATGAGCGTGGAAAG+AGG - Chr4:83515241-83515260 None:intergenic 50.0%
!! GTTTGGTGACGTTTGCATGG+TGG - Chr4:83515533-83515552 None:intergenic 50.0%
CAACCAGGACCTGTGCCAAA+GGG - Chr4:83515355-83515374 None:intergenic 55.0%
CACAAAGTTCGGGCATACGC+AGG + Chr4:83516803-83516822 MsG0480023300.01.T01:three_prime_UTR 55.0%
TACTCACCGGGGAACCTAGA+CGG + Chr4:83515739-83515758 MsG0480023300.01.T01:CDS 55.0%
TATCCCTTTGGCACAGGTCC+TGG + Chr4:83515349-83515368 MsG0480023300.01.T01:CDS 55.0%
TCAACTCCCCTTTGCCGTCT+AGG - Chr4:83515756-83515775 None:intergenic 55.0%
TGTTGTTGAGGCTGTGGGTG+TGG - Chr4:83515287-83515306 None:intergenic 55.0%
TTTGGCACAGGTCCTGGTTG+CGG + Chr4:83515355-83515374 MsG0480023300.01.T01:CDS 55.0%
CCTTTGCCGTCTAGGTTCCC+CGG - Chr4:83515748-83515767 None:intergenic 60.0%
CGGGGAACCTAGACGGCAAA+GGG + Chr4:83515746-83515765 MsG0480023300.01.T01:CDS 60.0%
CTGGAGTTCCGCATCTTGGC+TGG + Chr4:83516521-83516540 MsG0480023300.01.T01:CDS 60.0%
GCAACCAGGACCTGTGCCAA+AGG - Chr4:83515356-83515375 None:intergenic 60.0%
GCATACGCAGGGTCTGGGAA+AGG + Chr4:83516815-83516834 MsG0480023300.01.T01:three_prime_UTR 60.0%
GGGGAACCTAGACGGCAAAG+GGG + Chr4:83515747-83515766 MsG0480023300.01.T01:CDS 60.0%
GGGTGGTGTGAGTGTGAGAG+TGG - Chr4:83515494-83515513 None:intergenic 60.0%
TTCGGGCATACGCAGGGTCT+GGG + Chr4:83516810-83516829 MsG0480023300.01.T01:three_prime_UTR 60.0%
TTGGCACAGGTCCTGGTTGC+GGG + Chr4:83515356-83515375 MsG0480023300.01.T01:CDS 60.0%
! AATAGTGGGGACCCGCAACC+AGG - Chr4:83515370-83515389 None:intergenic 60.0%
CCGGGGAACCTAGACGGCAA+AGG + Chr4:83515745-83515764 MsG0480023300.01.T01:CDS 65.0%
GTTCGGGCATACGCAGGGTC+TGG + Chr4:83516809-83516828 MsG0480023300.01.T01:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 83515196 83517041 83515196 ID=MsG0480023300.01;Name=MsG0480023300.01
Chr4 mRNA 83515196 83517041 83515196 ID=MsG0480023300.01.T01;Parent=MsG0480023300.01;Name=MsG0480023300.01.T01;_AED=0.10;_eAED=0.15;_QI=18|0|0.66|1|0|0.33|3|476|335
Chr4 exon 83515196 83516078 83515196 ID=MsG0480023300.01.T01:exon:5907;Parent=MsG0480023300.01.T01
Chr4 exon 83516292 83516379 83516292 ID=MsG0480023300.01.T01:exon:5908;Parent=MsG0480023300.01.T01
Chr4 exon 83516511 83517041 83516511 ID=MsG0480023300.01.T01:exon:5909;Parent=MsG0480023300.01.T01
Chr4 five_prime_UTR 83515196 83515213 83515196 ID=MsG0480023300.01.T01:five_prime_utr;Parent=MsG0480023300.01.T01
Chr4 CDS 83515214 83516078 83515214 ID=MsG0480023300.01.T01:cds;Parent=MsG0480023300.01.T01
Chr4 CDS 83516292 83516379 83516292 ID=MsG0480023300.01.T01:cds;Parent=MsG0480023300.01.T01
Chr4 CDS 83516511 83516565 83516511 ID=MsG0480023300.01.T01:cds;Parent=MsG0480023300.01.T01
Chr4 three_prime_UTR 83516566 83517041 83516566 ID=MsG0480023300.01.T01:three_prime_utr;Parent=MsG0480023300.01.T01
Gene Sequence

>MsG0480023300.01.T01

ATGGCACTACCACCACTACTACCACCTCTTTCCACGCTCATCAACCTTTTCATGATTCTCATCTTAGCATCCACACCCACAGCCTCAACAACACTAACAGTGTGCCATGACACATGTGGCTCAATACAAGTCAAGTATCCCTTTGGCACAGGTCCTGGTTGCGGGTCCCCACTATTCAACCCATACATAACATGCACATCAAATGGAACAATAGACCAACTCATCCTCAAAACTCACACATCCTCATACCCTATAACCTCCATTTCATACACCACTTCCACTCTCACACTCACACCACCCTACATGTCTACTTGCACCACCATGCAAACGTCACCAAACTTTGGCATTGATTTAACTGCACCATTCCAAATATCTTCATCCACTTTCATCCTCCTCTCTTGCCAAGAAAAAAGTTCAACCATTTGTGACACTTCTTTTGATTACCTTTGTGCTTCTCTTTACTCTTGCCCTCAAGTAGTCTCACTTGGTATGCCTCTTTTTTCTCCTACTAACACATGTTGTGTATACTCACCGGGGAACCTAGACGGCAAAGGGGAGTTGAATCTTAGAGAGAATGATTGTAGTGGTTATACTTCGGTTGTGTCACTTGGTGATAACCCTACTGATCCTACTCATTGGGTTTATGGAGTGGCATTGAAATATACTCATGGTGCTTTTGATAACTTTGCAACCACTAAATGTACTAGTTGTGAGAGTAGTGGTGGTGTATGTGGATATGATCCTCCAGGAAGTGGATTTGTTTGTGTTTGTAAAGGTGGATATAATACTAGCCTTGATTGCTCTAATTACGATCAAAACCAAGATTTCCTTTGGGACTCTATCTCTCCTCCTCCCATGTACAACAAAAAAGGAAAAATAGATGGTTGGACGATGGAGATGTGTTTTTCTTATATAGCTTTAGAATATGTAAAAACAATATGCCAAAGTAATGGTTGGGAATGTCTGGAGTTCCGCATCTTGGCTGGTATCATTTTCAGCCTAGTGTAA
Protein sequence

>MsG0480023300.01.T01

MALPPLLPPLSTLINLFMILILASTPTASTTLTVCHDTCGSIQVKYPFGTGPGCGSPLFNPYITCTSNGTIDQLILKTHTSSYPITSISYTTSTLTLTPPYMSTCTTMQTSPNFGIDLTAPFQISSSTFILLSCQEKSSTICDTSFDYLCASLYSCPQVVSLGMPLFSPTNTCCVYSPGNLDGKGELNLRENDCSGYTSVVSLGDNPTDPTHWVYGVALKYTHGAFDNFATTKCTSCESSGGVCGYDPPGSGFVCVCKGGYNTSLDCSNYDQNQDFLWDSISPPPMYNKKGKIDGWTMEMCFSYIALEYVKTICQSNGWECLEFRILAGIIFSLV*