AlfalfaGEDB | MsG0480023507.01

M. sativa cultivar ZhongmuNo.1 / MsG0480023507.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023507.01.T01	XP_003630381.1	92.523	107	5	2	1	104	1	107	7.27E-56	178

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023507.01.T01	Q9ZUZ3	37.363	91	49	1	3	93	24	106	9.61E-18	75.5

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023507.01.T01	G7LFR3	92.523	107	5	2	1	104	1	107	3.47e-56	178

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0280011036.01	MsG0480023507.01	0.815095	1.101643e-51	7.523844e-49
MsG0480020797.01	MsG0480023507.01	-0.802793	4.739720e-49	2.342937e-46
MsG0480023375.01	MsG0480023507.01	0.804972	1.671254e-49	8.736746e-47
MsG0480023507.01	MsG0580027854.01	-0.806896	6.584593e-50	3.616131e-47
MsG0480023507.01	MsG0680035665.01	0.803279	3.760414e-49	1.881946e-46
MsG0480023507.01	MsG0780037052.01	-0.808075	3.700754e-50	2.095868e-47
MsG0480023507.01	MsG0880043495.01	-0.811542	6.644605e-51	4.124451e-48

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023507.01.T01	MTR_8g094980	92.523	107	5	2	1	104	1	107	8.81e-60	178
MsG0480023507.01.T01	MTR_3g097120	74.038	104	23	2	1	104	7	106	1.11e-42	135
MsG0480023507.01.T01	MTR_7g118010	40.698	86	42	1	3	88	23	99	6.57e-19	75.1
MsG0480023507.01.T01	MTR_3g092220	41.176	85	41	1	3	87	33	108	9.22e-18	72.8
MsG0480023507.01.T01	MTR_8g026730	37.647	85	44	1	3	87	27	102	9.84e-17	69.7
MsG0480023507.01.T01	MTR_4g124750	43.023	86	36	2	3	88	69	141	4.51e-14	64.3
MsG0480023507.01.T01	MTR_2g043960	39.535	86	41	1	3	88	76	150	1.34e-13	63.2
MsG0480023507.01.T01	MTR_4g072910	51.389	72	23	2	3	73	35	95	1.05e-12	59.3
MsG0480023507.01.T01	MTR_4g072300	42.857	77	32	2	2	77	25	90	1.10e-12	58.9
MsG0480023507.01.T01	MTR_4g072940	40.000	80	36	2	1	79	55	123	1.15e-12	59.7
MsG0480023507.01.T01	MTR_4g072220	41.250	80	35	2	1	79	24	92	1.27e-12	58.9
MsG0480023507.01.T01	MTR_4g072330	42.857	77	32	2	2	77	22	87	1.78e-12	58.5
MsG0480023507.01.T01	MTR_4g072890	41.026	78	34	2	1	77	24	90	2.04e-12	58.2
MsG0480023507.01.T01	MTR_7g405740	39.474	76	42	1	28	99	81	156	2.84e-12	59.7
MsG0480023507.01.T01	MTR_1g061570	46.377	69	24	2	3	71	19	74	3.09e-12	58.5
MsG0480023507.01.T01	MTR_4g072820	43.590	78	32	2	2	78	19	85	3.77e-12	57.4
MsG0480023507.01.T01	MTR_4g072240	41.558	77	33	2	2	77	25	90	5.17e-12	57.4
MsG0480023507.01.T01	MTR_3g098970	41.429	70	30	1	1	70	51	109	5.85e-12	58.5
MsG0480023507.01.T01	MTR_4g072250	41.772	79	33	2	1	78	24	90	6.86e-12	57.0
MsG0480023507.01.T01	MTR_4g072260	41.026	78	34	2	1	77	24	90	7.17e-12	57.0
MsG0480023507.01.T01	MTR_4g072500	43.836	73	29	2	2	73	24	85	1.19e-11	56.2
MsG0480023507.01.T01	MTR_3g084250	41.026	78	34	2	1	77	23	89	1.38e-11	56.2
MsG0480023507.01.T01	MTR_1g059770	40.000	80	37	2	6	85	52	120	2.12e-11	56.6
MsG0480023507.01.T01	MTR_8g096500	45.205	73	29	1	6	78	52	113	2.21e-11	57.4
MsG0480023507.01.T01	MTR_4g072640	44.444	72	28	2	3	73	25	85	2.28e-11	55.8
MsG0480023507.01.T01	MTR_4g072560	38.961	77	36	2	3	78	32	98	2.54e-11	55.8
MsG0480023507.01.T01	MTR_4g072870	43.836	73	29	2	2	73	25	86	2.64e-11	55.5
MsG0480023507.01.T01	MTR_5g008370	44.444	72	28	2	3	73	25	85	3.05e-11	56.6
MsG0480023507.01.T01	MTR_8g096440	45.205	73	29	1	6	78	48	109	3.44e-11	56.2
MsG0480023507.01.T01	MTR_4g072230	43.836	73	27	2	5	76	28	87	3.62e-11	55.1
MsG0480023507.01.T01	MTR_4g072660	40.541	74	32	2	1	73	18	80	4.66e-11	54.7
MsG0480023507.01.T01	MTR_8g096470	40.541	74	33	1	6	79	49	111	6.60e-11	55.1
MsG0480023507.01.T01	MTR_4g072880	39.744	78	35	2	1	77	24	90	7.09e-11	54.3
MsG0480023507.01.T01	MTR_4g072480	41.892	74	31	2	1	73	23	85	7.55e-11	54.3
MsG0480023507.01.T01	MTR_8g461420	43.836	73	29	2	1	72	23	84	7.97e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480023507.01.T01	AT2G46690	37.363	91	49	1	3	93	24	106	9.80e-19	75.5
MsG0480023507.01.T01	AT4G00880	39.759	83	41	1	2	84	27	100	3.93e-16	68.6
MsG0480023507.01.T01	AT5G53590	41.772	79	40	2	3	81	46	118	7.09e-16	68.6
MsG0480023507.01.T01	AT3G61900	40.000	80	40	1	3	82	30	101	1.17e-14	65.5
MsG0480023507.01.T01	AT5G20810	46.479	71	27	1	3	73	79	138	1.77e-12	60.5
MsG0480023507.01.T01	AT3G43120	43.421	76	32	1	3	78	79	143	3.16e-12	59.7
MsG0480023507.01.T01	AT5G20810	46.479	71	27	1	3	73	79	138	4.45e-12	59.7
MsG0480023507.01.T01	AT4G34780	40.000	80	39	2	1	79	26	97	7.42e-12	57.4
MsG0480023507.01.T01	AT4G34800	43.836	73	32	2	2	73	20	84	9.24e-12	56.6
MsG0480023507.01.T01	AT4G22620	37.333	75	39	1	3	77	79	145	9.83e-12	58.2
MsG0480023507.01.T01	AT4G34790	43.056	72	31	2	3	73	39	101	4.44e-11	55.5
MsG0480023507.01.T01	AT4G12410	34.667	75	41	1	3	77	76	142	4.92e-11	56.2

Find 23 sgRNAs with CRISPR-Local

Find 23 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATAAAGCTTATGAGATTTA+TGG	0.119621	4:-86239754	MsG0480023507.01.T01:CDS
GGATGATAAAGATATGAAAT+AGG	0.226231	4:+86239796	None:intergenic
ATGATGGTGGTGATGGTGAT+AGG	0.327003	4:+86239645	None:intergenic
CTAAAAGATGAAGGTGAAGA+AGG	0.393733	4:-86239899	None:intergenic
GATGATAAAGATATGAAATA+GGG	0.436771	4:+86239797	None:intergenic
GTGGTGATGGTGATAGGGTG+TGG	0.458211	4:+86239651	None:intergenic
TCAAGAAGACGCTTGAAAAG+AGG	0.477121	4:+86239775	None:intergenic
TAAAAGATGAAGGTGAAGAA+GGG	0.520859	4:-86239898	None:intergenic
AACGGAACAAGGAAGCTTGA+GGG	0.534951	4:+86239714	None:intergenic
ACAAAGCATGAGGAATCAAA+TGG	0.539588	4:+86239617	None:intergenic
GGTGATGATGATGGTGGTGA+TGG	0.544241	4:+86239638	None:intergenic
GAAGAACACTCTCAAGAAGA+TGG	0.549372	4:-86239847	MsG0480023507.01.T01:CDS
AGATGAAGGTGAAGAAGGGA+TGG	0.553458	4:-86239894	MsG0480023507.01.T01:CDS
TGATGGTGGTGATGGTGATA+GGG	0.561787	4:+86239646	None:intergenic
GAATCAAATGGTGATGATGA+TGG	0.574122	4:+86239629	None:intergenic
AGAAAATCATCAACGGAACA+AGG	0.574342	4:+86239703	None:intergenic
CAACGGAACAAGGAAGCTTG+AGG	0.591378	4:+86239713	None:intergenic
ATTTATGGCTACAACACTGA+TGG	0.615584	4:-86239739	MsG0480023507.01.T01:CDS
ACGGAACAAGGAAGCTTGAG+GGG	0.635480	4:+86239715	None:intergenic
GGATGGCTAGCAGTTCAAGT+AGG	0.650962	4:-86239877	MsG0480023507.01.T01:CDS
GAATTGAAATACAAAGCATG+AGG	0.674168	4:+86239607	None:intergenic
GAGGTGAAGAAAATCATCAA+CGG	0.680994	4:+86239696	None:intergenic
TCAAATGGTGATGATGATGG+TGG	0.690349	4:+86239632	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GATGATAAAGATATGAAATA+GGG	+	Chr4:86239698-86239717	None:intergenic	20.0%
!	GATAAAGCTTATGAGATTTA+TGG	-	Chr4:86239738-86239757	MsG0480023507.01.T01:CDS	25.0%
!	GGATGATAAAGATATGAAAT+AGG	+	Chr4:86239699-86239718	None:intergenic	25.0%
	GAATTGAAATACAAAGCATG+AGG	+	Chr4:86239888-86239907	None:intergenic	30.0%
	ACAAAGCATGAGGAATCAAA+TGG	+	Chr4:86239878-86239897	None:intergenic	35.0%
	AGAAAATCATCAACGGAACA+AGG	+	Chr4:86239792-86239811	None:intergenic	35.0%
	ATTTATGGCTACAACACTGA+TGG	-	Chr4:86239753-86239772	MsG0480023507.01.T01:CDS	35.0%
	GAGGTGAAGAAAATCATCAA+CGG	+	Chr4:86239799-86239818	None:intergenic	35.0%
!	GAATCAAATGGTGATGATGA+TGG	+	Chr4:86239866-86239885	None:intergenic	35.0%
!!!	ATTCTTTTTCAACTCGCCAA+CGG	+	Chr4:86239821-86239840	None:intergenic	35.0%
	GAAGAACACTCTCAAGAAGA+TGG	-	Chr4:86239645-86239664	MsG0480023507.01.T01:CDS	40.0%
	TCAAGAAGACGCTTGAAAAG+AGG	+	Chr4:86239720-86239739	None:intergenic	40.0%
!	ATGATTTTCTTCACCTCCGT+TGG	-	Chr4:86239802-86239821	MsG0480023507.01.T01:CDS	40.0%
!!	TCAAATGGTGATGATGATGG+TGG	+	Chr4:86239863-86239882	None:intergenic	40.0%
	AACGGAACAAGGAAGCTTGA+GGG	+	Chr4:86239781-86239800	None:intergenic	45.0%
!!	ATGATGGTGGTGATGGTGAT+AGG	+	Chr4:86239850-86239869	None:intergenic	45.0%
!!	TGATGGTGGTGATGGTGATA+GGG	+	Chr4:86239849-86239868	None:intergenic	45.0%
	ACGGAACAAGGAAGCTTGAG+GGG	+	Chr4:86239780-86239799	None:intergenic	50.0%
	CAACGGAACAAGGAAGCTTG+AGG	+	Chr4:86239782-86239801	None:intergenic	50.0%
	GGATGGCTAGCAGTTCAAGT+AGG	-	Chr4:86239615-86239634	MsG0480023507.01.T01:CDS	50.0%
!!	GGTGATGATGATGGTGGTGA+TGG	+	Chr4:86239857-86239876	None:intergenic	50.0%
!!!	CTTTTTCAACTCGCCAACGG+AGG	+	Chr4:86239818-86239837	None:intergenic	50.0%
!!	GTGGTGATGGTGATAGGGTG+TGG	+	Chr4:86239844-86239863	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	86239600	86239914	86239600	ID=MsG0480023507.01;Name=MsG0480023507.01
Chr4	mRNA	86239600	86239914	86239600	ID=MsG0480023507.01.T01;Parent=MsG0480023507.01;Name=MsG0480023507.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|104
Chr4	exon	86239600	86239914	86239600	ID=MsG0480023507.01.T01:exon:2972;Parent=MsG0480023507.01.T01
Chr4	CDS	86239600	86239914	86239600	ID=MsG0480023507.01.T01:cds;Parent=MsG0480023507.01.T01
Chr4	mRNA	86239632	86239766	86239632	ID=MsG0480023507.01.T02;Parent=MsG0480023507.01;Name=MsG0480023507.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|44
Chr4	exon	86239632	86239766	86239632	ID=MsG0480023507.01.T02:exon:2973;Parent=MsG0480023507.01.T02
Chr4	CDS	86239632	86239766	86239632	ID=MsG0480023507.01.T02:cds;Parent=MsG0480023507.01.T02

Gene Sequence

>MsG0480023507.01.T01

ATGAAGGTGAAGAAGGGATGGCTAGCAGTTCAAGTAGGTTCAGAAGAAGAACACTCTCAAGAAGATGGTGTAAGTGATTCTCAAAGATTTGTCATCCCTATTTCATATCTTTATCATCCTCTTTTCAAGCGTCTTCTTGATAAAGCTTATGAGATTTATGGCTACAACACTGATGGTCCCCTCAAGCTTCCTTGTTCCGTTGATGATTTTCTTCACCTCCGTTGGCGAGTTGAAAAAGAATCCACACCCTATCACCATCACCACCATCATCATCACCATTTGATTCCTCATGCTTTGTATTTCAATTCTTGTTGA

>MsG0480023507.01.T02

ATGAGATTTATGGCTACAACACTGATGGTCCCCTCAAGCTTCCTTGTTCCGTTGATGATTTTCTTCACCTCCGTTGGCGAGTTGAAAAAGAATCCACACCCTATCACCATCACCACCATCATCATCACCATTTGA

Protein sequence

>MsG0480023507.01.T01

MKVKKGWLAVQVGSEEEHSQEDGVSDSQRFVIPISYLYHPLFKRLLDKAYEIYGYNTDGPLKLPCSVDDFLHLRWRVEKESTPYHHHHHHHHHLIPHALYFNSC*

>MsG0480023507.01.T02

MRFMATTLMVPSSFLVPLMIFFTSVGELKKNPHPITITTIIITI*