AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480023721.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023721.01.T01 MTR_8g099835 98.649 148 2 0 25 172 5 152 5.00e-103 292
MsG0480023721.01.T01 MTR_2g105210 75.610 123 21 1 47 169 3 116 1.81e-58 180
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480023721.01.T01 AT4G34412 72.093 172 48 0 1 172 1 172 2.18e-85 248

Find 44 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATTATGATGAGATATTA+TGG 0.272998 4:-89000239 MsG0480023721.01.T01:three_prime_UTR
TGTTTCTTGCAGGGAACTAT+TGG 0.280671 4:-89001210 MsG0480023721.01.T01:intron
TAAATACATAATTACATGTT+TGG 0.329858 4:+89000826 None:intergenic
GCTAGAACAGGGAAAATATC+TGG 0.361800 4:+89000938 None:intergenic
TCCAATGACGAGTTTCTCTA+TGG 0.365136 4:+89000566 None:intergenic
GAGAAAGACTAACTTTGAAT+TGG 0.376103 4:+89002609 None:intergenic
AAGATATCTGCCTTGGAACT+TGG 0.384569 4:-89000406 MsG0480023721.01.T01:CDS
GCGTTGAGGAATGCAACTTC+TGG 0.407224 4:+89001161 None:intergenic
GCTCCTAGCCTTGTTGTGTA+AGG 0.407519 4:-89000295 MsG0480023721.01.T01:three_prime_UTR
CTCTCTCTTTCAGCTCCTCC+AGG 0.411924 4:+89000532 None:intergenic
AAGCATGCAAGCTGGGACAT+TGG 0.416752 4:-89001186 MsG0480023721.01.T01:CDS
TTGTGTGCAGCTGCTAGAAC+AGG 0.422005 4:+89000926 None:intergenic
GCCATAGAGAAACTCGTCAT+TGG 0.436847 4:-89000567 MsG0480023721.01.T01:CDS
TAGAATCTTCTCACCTCATC+AGG 0.442813 4:+89000663 None:intergenic
AAGCATCACGAGCAGCTATT+CGG 0.464580 4:+89000353 None:intergenic
AAATCGAGCAGCAAGGATAT+AGG 0.479316 4:+89000695 None:intergenic
GCATTACAAGATATCTGCCT+TGG 0.484266 4:-89000413 MsG0480023721.01.T01:CDS
AAGCTCATCGACGACATCAA+TGG 0.499776 4:-89002667 MsG0480023721.01.T01:CDS
GATGTCGTCGATGAGCTTCA+TGG 0.510473 4:+89002672 None:intergenic
AAATATGCTTTGAGACAAGA+TGG 0.516697 4:+89000189 None:intergenic
CTTGGAATATCATCACTTGA+AGG 0.518933 4:-89000388 MsG0480023721.01.T01:CDS
TATTGGAAAGCATGCAAGCT+GGG 0.540353 4:-89001193 MsG0480023721.01.T01:CDS
CTATTGGAAAGCATGCAAGC+TGG 0.542148 4:-89001194 MsG0480023721.01.T01:CDS
GGTCAAAGAGTCCCGTGACT+TGG 0.561465 4:+89000892 None:intergenic
GAGCTCGTTTACAACTACTC+AGG 0.561880 4:-89000851 MsG0480023721.01.T01:CDS
GAATCTGCCCCAGATACAAA+AGG 0.565073 4:-89000508 MsG0480023721.01.T01:intron
CATCCTTACACAACAAGGCT+AGG 0.579066 4:+89000292 None:intergenic
GACACTTGTAACCAAGTCAC+GGG 0.585819 4:-89000903 MsG0480023721.01.T01:CDS
TGAGTAGTTGTAAACGAGCT+CGG 0.589663 4:+89000853 None:intergenic
GTCGAACAGAACAGAAGAGA+AGG 0.598168 4:-89002707 MsG0480023721.01.T01:five_prime_UTR
TGATGATATTCCAAGTTCCA+AGG 0.603203 4:+89000396 None:intergenic
CATTGGAAAAGAGATTGACC+TGG 0.619360 4:-89000550 MsG0480023721.01.T01:CDS
AATTGGTGACGTCTGTGTAG+AGG 0.624172 4:+89002626 None:intergenic
AGACACTTGTAACCAAGTCA+CGG 0.624915 4:-89000904 MsG0480023721.01.T01:CDS
ATTATACAAGTGAAGCGTTG+AGG 0.626795 4:+89001147 None:intergenic
AGCTCGTTTACAACTACTCA+GGG 0.627963 4:-89000850 MsG0480023721.01.T01:CDS
ATTGGTGACGTCTGTGTAGA+GGG 0.634042 4:+89002627 None:intergenic
CTCGGAATGAAGAGTGCGTG+TGG 0.636758 4:+89000871 None:intergenic
TAAATCATCCTTACACAACA+AGG 0.647246 4:+89000287 None:intergenic
TGGAAAAGAGATTGACCTGG+AGG 0.655757 4:-89000547 MsG0480023721.01.T01:CDS
ATTTGATGCTACTCCTGATG+AGG 0.656826 4:-89000676 MsG0480023721.01.T01:intron
TGTGTGCAGCTGCTAGAACA+GGG 0.668610 4:+89000927 None:intergenic
TAGCATCAAATCGAGCAGCA+AGG 0.669820 4:+89000688 None:intergenic
GTAGAGGGAGAGACAGAGAG+TGG 0.695759 4:+89002642 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATAATTAACTTAAAAC+GGG + Chr4:89000457-89000476 None:intergenic 10.0%
!!! TATTTATATTTAACTTGTTA+TGG - Chr4:89002061-89002080 MsG0480023721.01.T01:intron 10.0%
!! AAAAAATAAAAAATTGGGTT+TGG + Chr4:89001027-89001046 None:intergenic 15.0%
!! TAAATACATAATTACATGTT+TGG + Chr4:89002047-89002066 None:intergenic 15.0%
!! TTAATTATGATGAGATATTA+TGG - Chr4:89002631-89002650 MsG0480023721.01.T01:CDS 15.0%
!!! GAGAAAATGATAATTTTTAT+AGG + Chr4:89000329-89000348 None:intergenic 15.0%
!! ATAAGAGCAATATATGAATT+TGG - Chr4:89000500-89000519 MsG0480023721.01.T01:intron 20.0%
!! CTATAAATAATAAGAGCTTA+TGG - Chr4:89000664-89000683 MsG0480023721.01.T01:intron 20.0%
!! GAAGATTCTACAATAAAATA+TGG - Chr4:89002220-89002239 MsG0480023721.01.T01:intron 20.0%
!! TGACAGTTTGAAAAATATTA+AGG + Chr4:89001148-89001167 None:intergenic 20.0%
!! TGTAATTGATCCATAAAATT+AGG - Chr4:89001604-89001623 MsG0480023721.01.T01:intron 20.0%
!! TTATGTAAGTGACAAAAATT+AGG + Chr4:89002248-89002267 None:intergenic 20.0%
!!! GTATATCTACTTTTATAGAT+TGG + Chr4:89001552-89001571 None:intergenic 20.0%
! AAATAATAAGAGCTTATGGA+TGG - Chr4:89000668-89000687 MsG0480023721.01.T01:intron 25.0%
! AAATTATTGAATACGCTAGT+TGG + Chr4:89002426-89002445 None:intergenic 25.0%
! AGCTCTTATTATTTATAGTG+TGG + Chr4:89000662-89000681 None:intergenic 25.0%
! ATGTTCTATTGTTATACTTG+TGG + Chr4:89000867-89000886 None:intergenic 25.0%
! CTGAAATTAGAAACTTTAAC+TGG + Chr4:89002096-89002115 None:intergenic 25.0%
! GTACTCTTACACTTTAAATT+TGG + Chr4:89000549-89000568 None:intergenic 25.0%
! TATATGTAACTATGCAACAT+AGG - Chr4:89001180-89001199 MsG0480023721.01.T01:CDS 25.0%
!! ACTGAATCTTTAAAAAGATG+TGG - Chr4:89002135-89002154 MsG0480023721.01.T01:intron 25.0%
!!! GTAATATTTTCAAGTGTTAG+TGG - Chr4:89000593-89000612 MsG0480023721.01.T01:intron 25.0%
ACTATGGTAAATGGTTATCA+AGG - Chr4:89001284-89001303 MsG0480023721.01.T01:intron 30.0%
AGATCCTCTATAAATGAATG+TGG + Chr4:89001759-89001778 None:intergenic 30.0%
AGTGTTAGTGGAGAAATTAT+AGG - Chr4:89000605-89000624 MsG0480023721.01.T01:intron 30.0%
ATAACATGATCCTCTTATGT+TGG - Chr4:89001804-89001823 MsG0480023721.01.T01:intron 30.0%
ATGTAACTATGTTTCTTGCA+GGG - Chr4:89001651-89001670 MsG0480023721.01.T01:intron 30.0%
CAATTCACAAGAGAATATGT+AGG - Chr4:89000716-89000735 MsG0480023721.01.T01:CDS 30.0%
CACTCAATAGCAATATTTGA+TGG + Chr4:89001330-89001349 None:intergenic 30.0%
CCAAATGTTGTGAATAGTTA+TGG + Chr4:89001418-89001437 None:intergenic 30.0%
CCATAACTATTCACAACATT+TGG - Chr4:89001415-89001434 MsG0480023721.01.T01:intron 30.0%
CTACATATTCTCTTGTGAAT+TGG + Chr4:89000718-89000737 None:intergenic 30.0%
GAGAAAGACTAACTTTGAAT+TGG + Chr4:89000264-89000283 None:intergenic 30.0%
GATCCTCTATAAATGAATGT+GGG + Chr4:89001758-89001777 None:intergenic 30.0%
GATGAGAAACACTTAATTCT+CGG + Chr4:89000435-89000454 None:intergenic 30.0%
GGAGAAATTATAGGAACATT+TGG - Chr4:89000614-89000633 MsG0480023721.01.T01:intron 30.0%
TAAATCATCCTTACACAACA+AGG + Chr4:89002586-89002605 None:intergenic 30.0%
TATACTTGTGGTAAGTACAA+GGG + Chr4:89000855-89000874 None:intergenic 30.0%
TATGTAACTATGTTTCTTGC+AGG - Chr4:89001650-89001669 MsG0480023721.01.T01:intron 30.0%
TTATACTTGTGGTAAGTACA+AGG + Chr4:89000856-89000875 None:intergenic 30.0%
!! AAATATGCTTTGAGACAAGA+TGG + Chr4:89002684-89002703 None:intergenic 30.0%
!!! ACTTTTTCACTTCTTTGGTA+TGG - Chr4:89001260-89001279 MsG0480023721.01.T01:intron 30.0%
!!! TCAACACTTTTTCACTTCTT+TGG - Chr4:89001255-89001274 MsG0480023721.01.T01:intron 30.0%
ACATAGGATAACTATCAACG+AGG - Chr4:89001196-89001215 MsG0480023721.01.T01:CDS 35.0%
ACGATGTCAAGTAAGTTCTT+TGG + Chr4:89000575-89000594 None:intergenic 35.0%
AGAACACTTTGTAGTAGATG+AGG - Chr4:89002715-89002734 MsG0480023721.01.T01:five_prime_UTR 35.0%
ATACAAGTCCTAGCATCATT+CGG + Chr4:89001842-89001861 None:intergenic 35.0%
ATGTAGCTAATCCCATTTAG+TGG - Chr4:89000905-89000924 MsG0480023721.01.T01:CDS 35.0%
CAGATACAAAAGGTTTGTCA+GGG - Chr4:89002372-89002391 MsG0480023721.01.T01:intron 35.0%
CTTGGAATATCATCACTTGA+AGG - Chr4:89002482-89002501 MsG0480023721.01.T01:intron 35.0%
TCTCTCATTACCAACATAAG+AGG + Chr4:89001817-89001836 None:intergenic 35.0%
TGACCCACATTCATTTATAG+AGG - Chr4:89001752-89001771 MsG0480023721.01.T01:intron 35.0%
TGATGATATTCCAAGTTCCA+AGG + Chr4:89002477-89002496 None:intergenic 35.0%
TGTAGCTAATCCCATTTAGT+GGG - Chr4:89000906-89000925 MsG0480023721.01.T01:CDS 35.0%
TTATAGTGTGGAGAGTTAGT+GGG + Chr4:89000650-89000669 None:intergenic 35.0%
TTTATAGTGTGGAGAGTTAG+TGG + Chr4:89000651-89000670 None:intergenic 35.0%
! CAAGTCTTATCCCACTAAAT+GGG + Chr4:89000919-89000938 None:intergenic 35.0%
! CTGACAAACCTTTTGTATCT+GGG + Chr4:89002373-89002392 None:intergenic 35.0%
! TGACAAACCTTTTGTATCTG+GGG + Chr4:89002372-89002391 None:intergenic 35.0%
! TTATCATTTTCTCTTACCGC+CGG - Chr4:89000336-89000355 MsG0480023721.01.T01:exon 35.0%
!! AGAGCTGGTTCCTAATTTTA+TGG + Chr4:89001617-89001636 None:intergenic 35.0%
!! ATTATACAAGTGAAGCGTTG+AGG + Chr4:89001726-89001745 None:intergenic 35.0%
!!! GTTGCTTTGCATTTTTAGTC+CGG - Chr4:89000390-89000409 MsG0480023721.01.T01:CDS 35.0%
AAATCGAGCAGCAAGGATAT+AGG + Chr4:89002178-89002197 None:intergenic 40.0%
AAGATATCTGCCTTGGAACT+TGG - Chr4:89002464-89002483 MsG0480023721.01.T01:intron 40.0%
ACAAGAGAATATGTAGGACG+AGG - Chr4:89000722-89000741 MsG0480023721.01.T01:CDS 40.0%
ACTGAAGCAATACAGTAAGC+CGG + Chr4:89000358-89000377 None:intergenic 40.0%
ACTTAATTCTCGGTCTGGAT+TGG + Chr4:89000425-89000444 None:intergenic 40.0%
ACTTCTTTGGTATGGCACTA+TGG - Chr4:89001268-89001287 MsG0480023721.01.T01:intron 40.0%
AGACACTTGTAACCAAGTCA+CGG - Chr4:89001966-89001985 MsG0480023721.01.T01:intron 40.0%
AGCTCGTTTACAACTACTCA+GGG - Chr4:89002020-89002039 MsG0480023721.01.T01:intron 40.0%
ATTTGATGCTACTCCTGATG+AGG - Chr4:89002194-89002213 MsG0480023721.01.T01:intron 40.0%
CAAGAGAATATGTAGGACGA+GGG - Chr4:89000723-89000742 MsG0480023721.01.T01:CDS 40.0%
CATTGGAAAAGAGATTGACC+TGG - Chr4:89002320-89002339 MsG0480023721.01.T01:intron 40.0%
CCAGATACAAAAGGTTTGTC+AGG - Chr4:89002371-89002390 MsG0480023721.01.T01:intron 40.0%
CCATCTTGATTAGGAGGAAT+CGG + Chr4:89001493-89001512 None:intergenic 40.0%
CCATTTAGTGGGATAAGACT+TGG - Chr4:89000917-89000936 MsG0480023721.01.T01:CDS 40.0%
GAAACACTTAATTCTCGGTC+TGG + Chr4:89000430-89000449 None:intergenic 40.0%
GCATTACAAGATATCTGCCT+TGG - Chr4:89002457-89002476 MsG0480023721.01.T01:intron 40.0%
GCTAGAACAGGGAAAATATC+TGG + Chr4:89001935-89001954 None:intergenic 40.0%
TAAACACTTCAGCTAAGAGC+TGG + Chr4:89001632-89001651 None:intergenic 40.0%
TAGAATCTTCTCACCTCATC+AGG + Chr4:89002210-89002229 None:intergenic 40.0%
TCCAATGACGAGTTTCTCTA+TGG + Chr4:89002307-89002326 None:intergenic 40.0%
TGAGTAGTTGTAAACGAGCT+CGG + Chr4:89002020-89002039 None:intergenic 40.0%
TGGTATGGCACTATGGTAAA+TGG - Chr4:89001275-89001294 MsG0480023721.01.T01:intron 40.0%
TGTTTCTTGCAGGGAACTAT+TGG - Chr4:89001660-89001679 MsG0480023721.01.T01:intron 40.0%
TTGCAATCGCCATCTTGATT+AGG + Chr4:89001502-89001521 None:intergenic 40.0%
! CAGGGAAAATATCTGGAATC+TGG + Chr4:89001928-89001947 None:intergenic 40.0%
! CCAAGTCTTATCCCACTAAA+TGG + Chr4:89000920-89000939 None:intergenic 40.0%
! CCTGACAAACCTTTTGTATC+TGG + Chr4:89002374-89002393 None:intergenic 40.0%
! TATTGGAAAGCATGCAAGCT+GGG - Chr4:89001677-89001696 MsG0480023721.01.T01:intron 40.0%
!! AGGAGTGTGCTTGATTTTAG+TGG - Chr4:89001304-89001323 MsG0480023721.01.T01:intron 40.0%
!! CTATTCACAACATTTGGTGC+TGG - Chr4:89001421-89001440 MsG0480023721.01.T01:intron 40.0%
!! CTCTTGTGAATTGGTGATGT+GGG + Chr4:89000709-89000728 None:intergenic 40.0%
!! TCTCTTGTGAATTGGTGATG+TGG + Chr4:89000710-89000729 None:intergenic 40.0%
!! TCTTGTGAATTGGTGATGTG+GGG + Chr4:89000708-89000727 None:intergenic 40.0%
!!! ATTTTGATTGTAGCGCGACA+GGG + Chr4:89000753-89000772 None:intergenic 40.0%
!!! TATTTTGATTGTAGCGCGAC+AGG + Chr4:89000754-89000773 None:intergenic 40.0%
!!! TTTGCATTTTTAGTCCGGAC+TGG - Chr4:89000395-89000414 MsG0480023721.01.T01:CDS 40.0%
AAGCATCACGAGCAGCTATT+CGG + Chr4:89002520-89002539 None:intergenic 45.0%
AAGCTCATCGACGACATCAA+TGG - Chr4:89000203-89000222 MsG0480023721.01.T01:three_prime_UTR 45.0%
CAATCGCCATCTTGATTAGG+AGG + Chr4:89001499-89001518 None:intergenic 45.0%
CATCCTTACACAACAAGGCT+AGG + Chr4:89002581-89002600 None:intergenic 45.0%
CCGATTCCTCCTAATCAAGA+TGG - Chr4:89001490-89001509 MsG0480023721.01.T01:intron 45.0%
GAATCTGCCCCAGATACAAA+AGG - Chr4:89002362-89002381 MsG0480023721.01.T01:intron 45.0%
GACACTTGTAACCAAGTCAC+GGG - Chr4:89001967-89001986 MsG0480023721.01.T01:intron 45.0%
GAGAGAATCCGAATGATGCT+AGG - Chr4:89001831-89001850 MsG0480023721.01.T01:intron 45.0%
GAGCTCGTTTACAACTACTC+AGG - Chr4:89002019-89002038 MsG0480023721.01.T01:intron 45.0%
GCCATAGAGAAACTCGTCAT+TGG - Chr4:89002303-89002322 MsG0480023721.01.T01:intron 45.0%
GTCGAACAGAACAGAAGAGA+AGG - Chr4:89000163-89000182 MsG0480023721.01.T01:three_prime_UTR 45.0%
TAGCATCAAATCGAGCAGCA+AGG + Chr4:89002185-89002204 None:intergenic 45.0%
TGGAAAAGAGATTGACCTGG+AGG - Chr4:89002323-89002342 MsG0480023721.01.T01:intron 45.0%
! CTATTGGAAAGCATGCAAGC+TGG - Chr4:89001676-89001695 MsG0480023721.01.T01:intron 45.0%
! GCTTTGAGCTAAAGGGATCT+TGG + Chr4:89000800-89000819 None:intergenic 45.0%
!! AATTGGTGACGTCTGTGTAG+AGG + Chr4:89000247-89000266 None:intergenic 45.0%
!! ATTGGTGACGTCTGTGTAGA+GGG + Chr4:89000246-89000265 None:intergenic 45.0%
!! GAGAGGAGCTTTGAGCTAAA+GGG + Chr4:89000807-89000826 None:intergenic 45.0%
!! TGAGAGGAGCTTTGAGCTAA+AGG + Chr4:89000808-89000827 None:intergenic 45.0%
!! AAGTTTAAAAAATAAAAAAT+TGG + Chr4:89001033-89001052 None:intergenic 5.0%
!! AGTTTAAAAAATAAAAAATT+GGG + Chr4:89001032-89001051 None:intergenic 5.0%
!! TAATAATAATTAACTTAAAA+CGG + Chr4:89000458-89000477 None:intergenic 5.0%
AAAGCTCCTCTCACCACCAA+GGG - Chr4:89000815-89000834 MsG0480023721.01.T01:intron 50.0%
AAGCATGCAAGCTGGGACAT+TGG - Chr4:89001684-89001703 MsG0480023721.01.T01:intron 50.0%
GAAGCAATACAGTAAGCCGG+CGG + Chr4:89000355-89000374 None:intergenic 50.0%
GATGTCGTCGATGAGCTTCA+TGG + Chr4:89000201-89000220 None:intergenic 50.0%
GCGTTGAGGAATGCAACTTC+TGG + Chr4:89001712-89001731 None:intergenic 50.0%
TCTGGTGCAGTTGAGAACAG+TGG + Chr4:89001910-89001929 None:intergenic 50.0%
TGTGTGCAGCTGCTAGAACA+GGG + Chr4:89001946-89001965 None:intergenic 50.0%
TTGTGTGCAGCTGCTAGAAC+AGG + Chr4:89001947-89001966 None:intergenic 50.0%
!! AATTCTCGGTCTGGATTGGC+CGG + Chr4:89000421-89000440 None:intergenic 50.0%
!! GCTCCTAGCCTTGTTGTGTA+AGG - Chr4:89002575-89002594 MsG0480023721.01.T01:intron 50.0%
!!! CATTTTTAGTCCGGACTGGC+CGG - Chr4:89000399-89000418 MsG0480023721.01.T01:CDS 50.0%
AAGCTCCTCTCACCACCAAG+GGG - Chr4:89000816-89000835 MsG0480023721.01.T01:intron 55.0%
CAAAGCTCCTCTCACCACCA+AGG - Chr4:89000814-89000833 MsG0480023721.01.T01:intron 55.0%
CAGCAGTGAGCAGTAAGGTC+AGG + Chr4:89000777-89000796 None:intergenic 55.0%
CTCTCTCTTTCAGCTCCTCC+AGG + Chr4:89002341-89002360 None:intergenic 55.0%
CTTGGCAGCAGTGAGCAGTA+AGG + Chr4:89000782-89000801 None:intergenic 55.0%
GAGAGTTAGTGGGAGTGGTG+AGG + Chr4:89000640-89000659 None:intergenic 55.0%
GGTCAAAGAGTCCCGTGACT+TGG + Chr4:89001981-89002000 None:intergenic 55.0%
GTAGAGGGAGAGACAGAGAG+TGG + Chr4:89000231-89000250 None:intergenic 55.0%
GTGTGGAGAGTTAGTGGGAG+TGG + Chr4:89000645-89000664 None:intergenic 55.0%
TTGATTGTAGCGCGACAGGG+AGG + Chr4:89000750-89000769 None:intergenic 55.0%
! CTCGGAATGAAGAGTGCGTG+TGG + Chr4:89002002-89002021 None:intergenic 55.0%
AGCTCCTCTCACCACCAAGG+GGG - Chr4:89000817-89000836 MsG0480023721.01.T01:intron 60.0%
CCTCTCACCACCAAGGGGGA+GGG - Chr4:89000821-89000840 MsG0480023721.01.T01:intron 65.0%
CTCTCACCACCAAGGGGGAG+GGG - Chr4:89000822-89000841 MsG0480023721.01.T01:intron 65.0%
TCCTCTCACCACCAAGGGGG+AGG - Chr4:89000820-89000839 MsG0480023721.01.T01:intron 65.0%
TCTCACCACCAAGGGGGAGG+GGG - Chr4:89000823-89000842 MsG0480023721.01.T01:intron 65.0%
!! TCTGGATTGGCCGGCCAGTC+CGG + Chr4:89000412-89000431 None:intergenic 65.0%
ATCGACCCCCTCCCCCTTGG+TGG + Chr4:89000831-89000850 None:intergenic 70.0%
!! CCCTCCCCCTTGGTGGTGAG+AGG + Chr4:89000824-89000843 None:intergenic 70.0%
!! GGCATCGACCCCCTCCCCCT+TGG + Chr4:89000834-89000853 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
Chr4 gene 89000158 89002734 89000158 ID=MsG0480023721.01;Name=MsG0480023721.01
Chr4 mRNA 89000158 89002734 89000158 ID=MsG0480023721.01.T01;Parent=MsG0480023721.01;Name=MsG0480023721.01.T01;_AED=0.49;_eAED=0.49;_QI=42|1|1|1|0.8|0.66|6|190|172
Chr4 exon 89000158 89000434 89000158 ID=MsG0480023721.01.T01:exon:25214;Parent=MsG0480023721.01.T01
Chr4 exon 89000509 89000595 89000509 ID=MsG0480023721.01.T01:exon:25213;Parent=MsG0480023721.01.T01
Chr4 exon 89000677 89000760 89000677 ID=MsG0480023721.01.T01:exon:25212;Parent=MsG0480023721.01.T01
Chr4 exon 89000841 89000963 89000841 ID=MsG0480023721.01.T01:exon:25211;Parent=MsG0480023721.01.T01
Chr4 exon 89001154 89001220 89001154 ID=MsG0480023721.01.T01:exon:25210;Parent=MsG0480023721.01.T01
Chr4 exon 89002622 89002734 89002622 ID=MsG0480023721.01.T01:exon:25209;Parent=MsG0480023721.01.T01
Chr4 five_prime_UTR 89002693 89002734 89002693 ID=MsG0480023721.01.T01:five_prime_utr;Parent=MsG0480023721.01.T01
Chr4 CDS 89002622 89002692 89002622 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 CDS 89001154 89001220 89001154 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 CDS 89000841 89000963 89000841 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 CDS 89000677 89000760 89000677 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 CDS 89000509 89000595 89000509 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 CDS 89000348 89000434 89000348 ID=MsG0480023721.01.T01:cds;Parent=MsG0480023721.01.T01
Chr4 three_prime_UTR 89000158 89000347 89000158 ID=MsG0480023721.01.T01:three_prime_utr;Parent=MsG0480023721.01.T01
Gene Sequence

>MsG0480023721.01.T01

ATGAAGCTCATCGACGACATCAATGGAACCACTCTCTGTCTCTCCCTCTACACAGACGTCACCAATTCAAAGGAACTATTGGAAAGCATGCAAGCTGGGACATTGGAGCCAGAAGTTGCATTCCTCAACGCTTCACTTATTCCAGATATTTTCCCTGTTCTAGCAGCTGCACACAAGACACTTGTAACCAAGTCACGGGACTCTTTGACCACACGCACTCTTCATTCCGAGCTCGTTTACAACTACTCAGGGTCCAAACATATTACTGAATCTTTAAAAAGATGTGGCATCTCTGAAAGCACAACCTATATCCTTGCTGCTCGATTTGATGCTACTCCTGATGAGATAAAAGCCATAGAGAAACTCGTCATTGGAAAAGAGATTGACCTGGAGGAGCTGAAAGAGAGAGCGAATCTGCCCCAGATACAAAAGCATTACAAGATATCTGCCTTGGAACTTGGAATATCATCACTTGAAGGTGCAATAACTTGCCGAATAGCTGCTCGTGATGCTTTGTAG
Protein sequence

>MsG0480023721.01.T01

MKLIDDINGTTLCLSLYTDVTNSKELLESMQAGTLEPEVAFLNASLIPDIFPVLAAAHKTLVTKSRDSLTTRTLHSELVYNYSGSKHITESLKRCGISESTTYILAARFDATPDEIKAIEKLVIGKEIDLEELKERANLPQIQKHYKISALELGISSLEGAITCRIAARDAL*