AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480021652.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021652.01.T01 MTR_4g077910 99.240 263 2 0 1 263 1 263 0.0 548
MsG0480021652.01.T01 MTR_1g039480 58.798 233 78 7 29 258 27 244 2.88e-83 270
MsG0480021652.01.T01 MTR_1g039445 61.712 222 62 6 29 247 20 221 3.32e-83 270
MsG0480021652.01.T01 MTR_5g029190 59.535 215 73 5 43 249 16 224 1.83e-75 249
MsG0480021652.01.T01 MTR_7g116230 56.962 158 29 4 106 263 1 119 4.83e-44 159
MsG0480021652.01.T01 MTR_7g109200 39.749 239 114 8 35 257 61 285 2.46e-37 140
MsG0480021652.01.T01 MTR_4g131780 46.988 166 56 5 16 180 11 145 2.53e-37 129
MsG0480021652.01.T01 MTR_2g438220 72.340 47 11 1 202 246 88 134 1.03e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0480021652.01.T01 AT3G03050 73.359 259 65 3 4 259 5 262 4.02e-131 399
MsG0480021652.01.T01 AT5G16910 75.104 241 59 1 9 248 10 250 7.69e-125 382
MsG0480021652.01.T01 AT4G38190 61.207 232 73 7 30 257 21 239 1.04e-86 280
MsG0480021652.01.T01 AT1G02730 39.147 258 122 8 10 249 36 276 1.38e-50 178
MsG0480021652.01.T01 AT2G33100 61.644 73 23 3 198 268 95 164 1.32e-18 85.9
MsG0480021652.01.T01 AT2G33100 51.429 70 26 3 14 78 9 75 3.41e-11 63.9
MsG0480021652.01.T01 AT2G33100 61.644 73 23 3 198 268 95 164 1.32e-18 85.9
MsG0480021652.01.T01 AT2G33100 51.429 70 26 3 14 78 9 75 3.41e-11 63.9

Find 75 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGTTCTGTGTTCTTATA+TGG 0.231354 4:-61288070 None:intergenic
TATATAGATGCAGTGAAAAT+AGG 0.334238 4:+61288019 MsG0480021652.01.T01:CDS
CCACAGATGGCTGGTGCAAA+AGG 0.351106 4:+61287893 MsG0480021652.01.T01:CDS
TGGCCAAAGGAAGGGGATTT+CGG 0.355911 4:+61288280 MsG0480021652.01.T01:CDS
TATTCCTGGCTGTGATTCTA+AGG 0.372247 4:+61287928 MsG0480021652.01.T01:CDS
TTCCACTTCCTCCACCAAAC+GGG 0.373965 4:+61288122 MsG0480021652.01.T01:CDS
CTGCTCATTTGTAATGAGTT+CGG 0.379209 4:-61287551 None:intergenic
TTATATGGCTCTTTGCATCC+TGG 0.379237 4:-61288055 None:intergenic
TATTACCCGTGCACATCTAA+TGG 0.387787 4:+61287841 MsG0480021652.01.T01:CDS
TTTGAAACAAAGGGGACATA+TGG 0.392709 4:+61288241 MsG0480021652.01.T01:CDS
GGGTCGAAATGTGCTATTCC+TGG 0.393677 4:+61287914 MsG0480021652.01.T01:CDS
TATATGGCTCTTTGCATCCT+GGG 0.396880 4:-61288054 None:intergenic
TTTCCGAAATCCCCTTCCTT+TGG 0.396984 4:-61288283 None:intergenic
AAATGTTACGGTTGGATGTA+AGG 0.415264 4:-61287621 None:intergenic
AAGGAAGGGGATTTCGGAAA+TGG 0.422255 4:+61288286 MsG0480021652.01.T01:CDS
CGATGGTTGTTTGAAACAAA+GGG 0.424075 4:+61288232 MsG0480021652.01.T01:CDS
CTCGCTATCAAGATCATCTC+TGG 0.432065 4:-61287681 None:intergenic
TTACATCCAACCGTAACATT+TGG 0.435589 4:+61287623 MsG0480021652.01.T01:CDS
GTTTCAAGTTCTCTATTCAC+TGG 0.442653 4:+61287806 MsG0480021652.01.T01:CDS
GATGAAGTGGCTGTGAATAA+TGG 0.446503 4:+61288091 MsG0480021652.01.T01:CDS
GTATCCATAGGTTGGTTATC+CGG 0.451756 4:-61287752 None:intergenic
ATGGTTATGGCAATGCAATA+TGG 0.453385 4:+61288260 MsG0480021652.01.T01:CDS
CTCAACTTTCTGCGAGATTG+AGG 0.454526 4:-61287774 None:intergenic
CTTCCACTTCCTCCACCAAA+CGG 0.457767 4:+61288121 MsG0480021652.01.T01:CDS
TGCAATATGGCCAAAGGAAG+GGG 0.469151 4:+61288273 MsG0480021652.01.T01:CDS
GCGCTGATGAGAAGTCAAAC+TGG 0.475372 4:+61288193 MsG0480021652.01.T01:CDS
AGTTCTTCGGCCAAATGTTA+CGG 0.477640 4:-61287633 None:intergenic
ACAAAGGGGACATATGGTTA+TGG 0.484592 4:+61288247 MsG0480021652.01.T01:CDS
GATGATCTTGATAGCGAGCT+AGG 0.485096 4:+61287686 MsG0480021652.01.T01:CDS
TATCTTATAATCACACTCAC+AGG 0.494707 4:-61287984 None:intergenic
TTTGGTGGAGGAAGTGGAAG+TGG 0.501359 4:-61288118 None:intergenic
TCGATGGTTGTTTGAAACAA+AGG 0.518208 4:+61288231 MsG0480021652.01.T01:CDS
CTTCGGCCAAATGTTACGGT+TGG 0.525516 4:-61287629 None:intergenic
GAACACAGAACTTGATGAAG+TGG 0.527152 4:+61288078 MsG0480021652.01.T01:CDS
ATCGACCGGATGAAGTTCTT+CGG 0.533914 4:-61287646 None:intergenic
CCACTGATTGATGAGCCGCC+AGG 0.539087 4:+61288331 MsG0480021652.01.T01:CDS
CACAGATGGCTGGTGCAAAA+GGG 0.540028 4:+61287894 MsG0480021652.01.T01:CDS
AGACTTTGTCGATTTCATCA+AGG 0.542739 4:-61288170 None:intergenic
ATAGGAGATGGGATGTGCCC+AGG 0.542804 4:+61288037 MsG0480021652.01.T01:CDS
ATGCAGTGAAAATAGGAGAT+GGG 0.544148 4:+61288026 MsG0480021652.01.T01:CDS
GATGCAGTGAAAATAGGAGA+TGG 0.544653 4:+61288025 MsG0480021652.01.T01:CDS
CTTTATCCATTAGATGTGCA+CGG 0.545565 4:-61287847 None:intergenic
TTTGGCCGAAGAACTTCATC+CGG 0.545571 4:+61287641 MsG0480021652.01.T01:CDS
GTAAATCATCCACAGATGGC+TGG 0.551375 4:+61287884 MsG0480021652.01.T01:CDS
GATTTCGGAAATGGAAAAGA+TGG 0.553684 4:+61288295 MsG0480021652.01.T01:CDS
TTTATCCATTAGATGTGCAC+GGG 0.557966 4:-61287846 None:intergenic
CACTCCGGATAACCAACCTA+TGG 0.561400 4:+61287748 MsG0480021652.01.T01:CDS
TCCACTTCCTCCACCAAACG+GGG 0.562983 4:+61288123 MsG0480021652.01.T01:CDS
TCAAGTTCTCTATTCACTGG+AGG 0.565971 4:+61287809 MsG0480021652.01.T01:CDS
TGCGAGATTGAGGTATCCAT+AGG 0.569033 4:-61287764 None:intergenic
AGATTGAGGTATCCATAGGT+TGG 0.571127 4:-61287760 None:intergenic
TCGGCTCATCAATTCAGTCC+TGG 0.574846 4:-61288349 None:intergenic
AATGTTACGGTTGGATGTAA+GGG 0.578477 4:-61287620 None:intergenic
ATCACCTTAGAATCACAGCC+AGG 0.581701 4:-61287932 None:intergenic
CATAGGTTGGTTATCCGGAG+TGG 0.589102 4:-61287747 None:intergenic
ATAGGTTGGTTATCCGGAGT+GGG 0.590129 4:-61287746 None:intergenic
GGAGTAACTGATGTATCGAC+CGG 0.593019 4:-61287660 None:intergenic
AATGCAATATGGCCAAAGGA+AGG 0.593345 4:+61288271 MsG0480021652.01.T01:CDS
ATGCAATATGGCCAAAGGAA+GGG 0.595431 4:+61288272 MsG0480021652.01.T01:CDS
CTCAATCTCGCAGAAAGTTG+AGG 0.597849 4:+61287775 MsG0480021652.01.T01:CDS
TGAAGTAAATCATCCACAGA+TGG 0.601911 4:+61287880 MsG0480021652.01.T01:CDS
CACTTCCTCCACCAAACGGG+GGG 0.610575 4:+61288125 MsG0480021652.01.T01:CDS
TGGCAATGCAATATGGCCAA+AGG 0.629077 4:+61288267 MsG0480021652.01.T01:CDS
GTTACGGTTGGATGTAAGGG+AGG 0.629747 4:-61287617 None:intergenic
CCACTTCCTCCACCAAACGG+GGG 0.633334 4:+61288124 MsG0480021652.01.T01:CDS
AGTGCACTTACCACCCACTC+CGG 0.636922 4:+61287733 MsG0480021652.01.T01:CDS
GAGACTTTGATCACAATCGA+TGG 0.649293 4:+61288215 MsG0480021652.01.T01:CDS
CCTGGCGGCTCATCAATCAG+TGG 0.652858 4:-61288331 None:intergenic
GGTTGGTTATCCGGAGTGGG+TGG 0.659629 4:-61287743 None:intergenic
GAAGTGGCTGTGAATAATGG+AGG 0.663961 4:+61288094 MsG0480021652.01.T01:CDS
ATCTTATAATCACACTCACA+GGG 0.682477 4:-61287983 None:intergenic
GATGGTTGTTTGAAACAAAG+GGG 0.686620 4:+61288233 MsG0480021652.01.T01:CDS
AAGTGGCTGTGAATAATGGA+GGG 0.736494 4:+61288095 MsG0480021652.01.T01:CDS
GCTCATCAATTCAGTCCTGG+CGG 0.742065 4:-61288346 None:intergenic
AAGGTGATGAGTGATGAACG+TGG 0.906383 4:+61287947 MsG0480021652.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! TATATAGATGCAGTGAAAAT+AGG + Chr4:61288019-61288038 MsG0480021652.01.T01:CDS 25.0%
ATCTTATAATCACACTCACA+GGG - Chr4:61287986-61288005 None:intergenic 30.0%
TATCTTATAATCACACTCAC+AGG - Chr4:61287987-61288006 None:intergenic 30.0%
TCAAGTTCTGTGTTCTTATA+TGG - Chr4:61288073-61288092 None:intergenic 30.0%
AAATGTTACGGTTGGATGTA+AGG - Chr4:61287624-61287643 None:intergenic 35.0%
AATGTTACGGTTGGATGTAA+GGG - Chr4:61287623-61287642 None:intergenic 35.0%
ATGCAGTGAAAATAGGAGAT+GGG + Chr4:61288026-61288045 MsG0480021652.01.T01:CDS 35.0%
ATGGTTATGGCAATGCAATA+TGG + Chr4:61288260-61288279 MsG0480021652.01.T01:CDS 35.0%
CTGCTCATTTGTAATGAGTT+CGG - Chr4:61287554-61287573 None:intergenic 35.0%
CTTTATCCATTAGATGTGCA+CGG - Chr4:61287850-61287869 None:intergenic 35.0%
GATTTCGGAAATGGAAAAGA+TGG + Chr4:61288295-61288314 MsG0480021652.01.T01:CDS 35.0%
TGAAGTAAATCATCCACAGA+TGG + Chr4:61287880-61287899 MsG0480021652.01.T01:CDS 35.0%
TTACATCCAACCGTAACATT+TGG + Chr4:61287623-61287642 MsG0480021652.01.T01:CDS 35.0%
TTTATCCATTAGATGTGCAC+GGG - Chr4:61287849-61287868 None:intergenic 35.0%
! CGATGGTTGTTTGAAACAAA+GGG + Chr4:61288232-61288251 MsG0480021652.01.T01:CDS 35.0%
! GATGGTTGTTTGAAACAAAG+GGG + Chr4:61288233-61288252 MsG0480021652.01.T01:CDS 35.0%
! TCGATGGTTGTTTGAAACAA+AGG + Chr4:61288231-61288250 MsG0480021652.01.T01:CDS 35.0%
! TTTGAAACAAAGGGGACATA+TGG + Chr4:61288241-61288260 MsG0480021652.01.T01:CDS 35.0%
!! AGACTTTGTCGATTTCATCA+AGG - Chr4:61288173-61288192 None:intergenic 35.0%
!! GTTTCAAGTTCTCTATTCAC+TGG + Chr4:61287806-61287825 MsG0480021652.01.T01:CDS 35.0%
AATGCAATATGGCCAAAGGA+AGG + Chr4:61288271-61288290 MsG0480021652.01.T01:CDS 40.0%
ACAAAGGGGACATATGGTTA+TGG + Chr4:61288247-61288266 MsG0480021652.01.T01:CDS 40.0%
AGTTCTTCGGCCAAATGTTA+CGG - Chr4:61287636-61287655 None:intergenic 40.0%
ATGCAATATGGCCAAAGGAA+GGG + Chr4:61288272-61288291 MsG0480021652.01.T01:CDS 40.0%
GAACACAGAACTTGATGAAG+TGG + Chr4:61288078-61288097 MsG0480021652.01.T01:CDS 40.0%
GAGACTTTGATCACAATCGA+TGG + Chr4:61288215-61288234 MsG0480021652.01.T01:CDS 40.0%
GATGAAGTGGCTGTGAATAA+TGG + Chr4:61288091-61288110 MsG0480021652.01.T01:CDS 40.0%
GATGCAGTGAAAATAGGAGA+TGG + Chr4:61288025-61288044 MsG0480021652.01.T01:CDS 40.0%
TATATGGCTCTTTGCATCCT+GGG - Chr4:61288057-61288076 None:intergenic 40.0%
TATTACCCGTGCACATCTAA+TGG + Chr4:61287841-61287860 MsG0480021652.01.T01:CDS 40.0%
TATTCCTGGCTGTGATTCTA+AGG + Chr4:61287928-61287947 MsG0480021652.01.T01:CDS 40.0%
TCAAGTTCTCTATTCACTGG+AGG + Chr4:61287809-61287828 MsG0480021652.01.T01:CDS 40.0%
TTATATGGCTCTTTGCATCC+TGG - Chr4:61288058-61288077 None:intergenic 40.0%
! AAGTGGCTGTGAATAATGGA+GGG + Chr4:61288095-61288114 MsG0480021652.01.T01:CDS 40.0%
! AGATTGAGGTATCCATAGGT+TGG - Chr4:61287763-61287782 None:intergenic 40.0%
!! GTATCCATAGGTTGGTTATC+CGG - Chr4:61287755-61287774 None:intergenic 40.0%
AAGGAAGGGGATTTCGGAAA+TGG + Chr4:61288286-61288305 MsG0480021652.01.T01:CDS 45.0%
AAGGTGATGAGTGATGAACG+TGG + Chr4:61287947-61287966 MsG0480021652.01.T01:CDS 45.0%
ATCACCTTAGAATCACAGCC+AGG - Chr4:61287935-61287954 None:intergenic 45.0%
ATCGACCGGATGAAGTTCTT+CGG - Chr4:61287649-61287668 None:intergenic 45.0%
CTCAACTTTCTGCGAGATTG+AGG - Chr4:61287777-61287796 None:intergenic 45.0%
CTCAATCTCGCAGAAAGTTG+AGG + Chr4:61287775-61287794 MsG0480021652.01.T01:CDS 45.0%
CTCGCTATCAAGATCATCTC+TGG - Chr4:61287684-61287703 None:intergenic 45.0%
GAAGTGGCTGTGAATAATGG+AGG + Chr4:61288094-61288113 MsG0480021652.01.T01:CDS 45.0%
GTAAATCATCCACAGATGGC+TGG + Chr4:61287884-61287903 MsG0480021652.01.T01:CDS 45.0%
TGCAATATGGCCAAAGGAAG+GGG + Chr4:61288273-61288292 MsG0480021652.01.T01:CDS 45.0%
TGGCAATGCAATATGGCCAA+AGG + Chr4:61288267-61288286 MsG0480021652.01.T01:CDS 45.0%
TTTCCGAAATCCCCTTCCTT+TGG - Chr4:61288286-61288305 None:intergenic 45.0%
TTTGGCCGAAGAACTTCATC+CGG + Chr4:61287641-61287660 MsG0480021652.01.T01:CDS 45.0%
! GGAGTAACTGATGTATCGAC+CGG - Chr4:61287663-61287682 None:intergenic 45.0%
! TGCGAGATTGAGGTATCCAT+AGG - Chr4:61287767-61287786 None:intergenic 45.0%
!! ATAGGTTGGTTATCCGGAGT+GGG - Chr4:61287749-61287768 None:intergenic 45.0%
!! GATGATCTTGATAGCGAGCT+AGG + Chr4:61287686-61287705 MsG0480021652.01.T01:CDS 45.0%
ACCAAACGGGGGGTCTAAAA+TGG + Chr4:61288135-61288154 MsG0480021652.01.T01:CDS 50.0%
CACAGATGGCTGGTGCAAAA+GGG + Chr4:61287894-61287913 MsG0480021652.01.T01:CDS 50.0%
CACTCCGGATAACCAACCTA+TGG + Chr4:61287748-61287767 MsG0480021652.01.T01:CDS 50.0%
CTTCCACTTCCTCCACCAAA+CGG + Chr4:61288121-61288140 MsG0480021652.01.T01:CDS 50.0%
CTTCGGCCAAATGTTACGGT+TGG - Chr4:61287632-61287651 None:intergenic 50.0%
GTTACGGTTGGATGTAAGGG+AGG - Chr4:61287620-61287639 None:intergenic 50.0%
TGGCCAAAGGAAGGGGATTT+CGG + Chr4:61288280-61288299 MsG0480021652.01.T01:CDS 50.0%
TTCCACTTCCTCCACCAAAC+GGG + Chr4:61288122-61288141 MsG0480021652.01.T01:CDS 50.0%
TTTGGTGGAGGAAGTGGAAG+TGG - Chr4:61288121-61288140 None:intergenic 50.0%
! ATTTTAGACCCCCCGTTTGG+TGG - Chr4:61288136-61288155 None:intergenic 50.0%
! GCGCTGATGAGAAGTCAAAC+TGG + Chr4:61288193-61288212 MsG0480021652.01.T01:CDS 50.0%
! GGGTCGAAATGTGCTATTCC+TGG + Chr4:61287914-61287933 MsG0480021652.01.T01:CDS 50.0%
! TCCATTTTAGACCCCCCGTT+TGG - Chr4:61288139-61288158 None:intergenic 50.0%
!! CATAGGTTGGTTATCCGGAG+TGG - Chr4:61287750-61287769 None:intergenic 50.0%
AGTGCACTTACCACCCACTC+CGG + Chr4:61287733-61287752 MsG0480021652.01.T01:CDS 55.0%
ATAGGAGATGGGATGTGCCC+AGG + Chr4:61288037-61288056 MsG0480021652.01.T01:CDS 55.0%
CCACAGATGGCTGGTGCAAA+AGG + Chr4:61287893-61287912 MsG0480021652.01.T01:CDS 55.0%
TCCACTTCCTCCACCAAACG+GGG + Chr4:61288123-61288142 MsG0480021652.01.T01:CDS 55.0%
! ACGGGGGGTCTAAAATGGAG+AGG + Chr4:61288140-61288159 MsG0480021652.01.T01:CDS 55.0%
! CCTTTTGCACCAGCCATCTG+TGG - Chr4:61287896-61287915 None:intergenic 55.0%
CACTTCCTCCACCAAACGGG+GGG + Chr4:61288125-61288144 MsG0480021652.01.T01:CDS 60.0%
CCACTTCCTCCACCAAACGG+GGG + Chr4:61288124-61288143 MsG0480021652.01.T01:CDS 60.0%
CCTGGCGGCTCATCAATCAG+TGG - Chr4:61288334-61288353 None:intergenic 60.0%
! CCACTGATTGATGAGCCGCC+AGG + Chr4:61288331-61288350 MsG0480021652.01.T01:CDS 60.0%
! GGTTGGTTATCCGGAGTGGG+TGG - Chr4:61287746-61287765 None:intergenic 60.0%
! TTAGACCCCCCGTTTGGTGG+AGG - Chr4:61288133-61288152 None:intergenic 60.0%
! CCCCCGTTTGGTGGAGGAAG+TGG - Chr4:61288127-61288146 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr4 gene 61287545 61288363 61287545 ID=MsG0480021652.01;Name=MsG0480021652.01
Chr4 mRNA 61287545 61288363 61287545 ID=MsG0480021652.01.T01;Parent=MsG0480021652.01;Name=MsG0480021652.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|272
Chr4 exon 61287545 61288363 61287545 ID=MsG0480021652.01.T01:exon:9553;Parent=MsG0480021652.01.T01
Chr4 CDS 61287545 61288363 61287545 ID=MsG0480021652.01.T01:cds;Parent=MsG0480021652.01.T01
Gene Sequence

>MsG0480021652.01.T01

ATGAAGCCGAACTCATTACAAATGAGCAGATCATCTTTGTCAACATCTTCTGATGTATCTGAAGCACAGAAGCCTCCCTTACATCCAACCGTAACATTTGGCCGAAGAACTTCATCCGGTCGATACATCAGTTACTCCAGAGATGATCTTGATAGCGAGCTAGGAAGTAATGACTTCATGAACTATACAGTGCACTTACCACCCACTCCGGATAACCAACCTATGGATACCTCAATCTCGCAGAAAGTTGAGGAGCAGTATGTTTCAAGTTCTCTATTCACTGGAGGATTTAACAGTATTACCCGTGCACATCTAATGGATAAAGTTACAGAATCTGAAGTAAATCATCCACAGATGGCTGGTGCAAAAGGGTCGAAATGTGCTATTCCTGGCTGTGATTCTAAGGTGATGAGTGATGAACGTGGTGACGACATTCTTCCCTGTGAGTGTGATTATAAGATATGTAGAGATTGCTATATAGATGCAGTGAAAATAGGAGATGGGATGTGCCCAGGATGCAAAGAGCCATATAAGAACACAGAACTTGATGAAGTGGCTGTGAATAATGGAGGGCCACTTCCACTTCCTCCACCAAACGGGGGGTCTAAAATGGAGAGGAGATTGTCCTTGATGAAATCGACAAAGTCTGCGCTGATGAGAAGTCAAACTGGAGACTTTGATCACAATCGATGGTTGTTTGAAACAAAGGGGACATATGGTTATGGCAATGCAATATGGCCAAAGGAAGGGGATTTCGGAAATGGAAAAGATGGTGATGTTTCTGAGCCACTGATTGATGAGCCGCCAGGACTGAATTGA

Protein sequence

>MsG0480021652.01.T01

MKPNSLQMSRSSLSTSSDVSEAQKPPLHPTVTFGRRTSSGRYISYSRDDLDSELGSNDFMNYTVHLPPTPDNQPMDTSISQKVEEQYVSSSLFTGGFNSITRAHLMDKVTESEVNHPQMAGAKGSKCAIPGCDSKVMSDERGDDILPCECDYKICRDCYIDAVKIGDGMCPGCKEPYKNTELDEVAVNNGGPLPLPPPNGGSKMERRLSLMKSTKSALMRSQTGDFDHNRWLFETKGTYGYGNAIWPKEGDFGNGKDGDVSEPLIDEPPGLN*