AlfalfaGEDB | MsG0580024143.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024143.01

KEGG: -
GO: GO:0005515
PFAM: PF01535 PF13041

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024143.01.T01	RHN52626.1	39.011	182	102	3	1	174	44	224	6.65E-37	142
MsG0580024143.01.T01	RHN52626.1	28.659	164	112	2	3	162	151	313	2.88E-16	87.4
MsG0580024143.01.T01	RHN52626.1	26.506	166	117	2	3	164	186	350	3.26E-11	73.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024143.01.T01	Q9C8T7	35.556	180	107	3	3	174	329	507	1.34E-22	97.8

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024143.01.T01	A0A396HH95	39.011	182	102	3	1	174	44	224	3.18e-37	142

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000470.01	MsG0580024143.01	0.810613	1.056499e-50	6.398300e-48
MsG0180003673.01	MsG0580024143.01	0.800136	1.660111e-48	7.670973e-46
MsG0180003686.01	MsG0580024143.01	0.801908	7.210325e-49	3.484697e-46
MsG0180004465.01	MsG0580024143.01	0.810461	1.139761e-50	6.874295e-48
MsG0180006134.01	MsG0580024143.01	0.835194	1.933927e-56	2.347736e-53
MsG0280007955.01	MsG0580024143.01	0.812753	3.618117e-51	2.319566e-48
MsG0280008112.01	MsG0580024143.01	0.810243	1.269616e-50	7.613967e-48
MsG0280008113.01	MsG0580024143.01	0.805063	1.599845e-49	8.382801e-47
MsG0280009421.01	MsG0580024143.01	0.844558	7.017796e-59	1.137362e-55
MsG0280010349.01	MsG0580024143.01	0.811678	6.209273e-51	3.867667e-48

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024143.01.T01	MTR_0237s0070	39.011	182	102	3	1	174	335	515	1.73e-39	143
MsG0580024143.01.T01	MTR_0237s0070	28.659	164	112	2	3	162	442	604	5.02e-20	87.8
MsG0580024143.01.T01	MTR_0237s0070	26.829	164	115	2	3	162	477	639	4.19e-15	73.6
MsG0580024143.01.T01	MTR_0237s0070	25.000	164	118	2	3	162	512	674	2.09e-12	65.5
MsG0580024143.01.T01	MTR_6g079180	35.321	218	117	5	1	197	44	258	2.57e-39	138
MsG0580024143.01.T01	MTR_6g079180	27.439	164	114	2	3	162	151	313	1.11e-17	80.1
MsG0580024143.01.T01	MTR_6g079180	27.778	162	112	2	5	162	188	348	1.60e-14	71.2
MsG0580024143.01.T01	MTR_6g079180	23.649	148	108	2	3	146	221	367	2.74e-11	62.0
MsG0580024143.01.T01	MTR_6g079210	35.321	218	117	5	1	197	44	258	2.70e-39	138
MsG0580024143.01.T01	MTR_6g079210	27.439	164	114	2	3	162	151	313	6.16e-18	80.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024143.01.T01	AT1G63330	35.556	180	107	3	3	174	329	507	1.37e-23	97.8
MsG0580024143.01.T01	AT1G63330	30.000	180	117	3	3	174	259	437	1.97e-21	91.7
MsG0580024143.01.T01	AT1G63330	29.121	182	120	3	1	174	187	367	3.13e-20	88.2
MsG0580024143.01.T01	AT1G63330	25.556	180	125	3	3	174	49	227	3.39e-14	70.9
MsG0580024143.01.T01	AT1G63330	25.455	165	118	2	2	162	83	246	2.21e-11	62.4
MsG0580024143.01.T01	AT1G63330	35.556	180	107	3	3	174	329	507	1.37e-23	97.8
MsG0580024143.01.T01	AT1G63330	30.000	180	117	3	3	174	259	437	1.97e-21	91.7
MsG0580024143.01.T01	AT1G63330	29.121	182	120	3	1	174	187	367	3.13e-20	88.2
MsG0580024143.01.T01	AT1G63330	25.556	180	125	3	3	174	49	227	3.39e-14	70.9
MsG0580024143.01.T01	AT1G63330	25.455	165	118	2	2	162	83	246	2.21e-11	62.4

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Find 36 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATGTTAAATGGTTTGTGCA+AGG	0.636305	5:+1305364	MsG0580024143.01.T01:CDS
AATTGATGGGTTCTCGATAG+AGG	0.464591	5:+1305157	MsG0580024143.01.T01:CDS
ACTGATGGATGTTGCAGGAA+TGG	0.543797	5:-1305498	None:intergenic
AGTGAAAAGCATCTTATTCA+TGG	0.353660	5:+1305017	MsG0580024143.01.T01:CDS
ATATTGCCTAATTCATAAGA+TGG	0.521295	5:+1305073	MsG0580024143.01.T01:CDS
ATCAACATGATGCTCAAAGA+GGG	0.537955	5:+1305206	MsG0580024143.01.T01:CDS
ATGCTGAATACTCTTGTTGA+TGG	0.401537	5:+1304942	MsG0580024143.01.T01:CDS
ATGGGGAAATTGTGTTTGCT+AGG	0.495735	5:-1305479	None:intergenic
ATTGATGGGTTCTCGATAGA+GGG	0.577796	5:+1305158	MsG0580024143.01.T01:CDS
CAAGGCCAATTTGATTGGTG+AGG	0.634270	5:+1305382	MsG0580024143.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATGAATTAGGCAATATCCA+TGG	-	Chr5:1305070-1305089	None:intergenic	30.0%
TTACTTCACTGTTAGTAATG+TGG	+	Chr5:1305271-1305290	MsG0580024143.01.T01:CDS	30.0%
TTGTGTCCATCTTATGAATT+AGG	-	Chr5:1305082-1305101	None:intergenic	30.0%
ATCAACATGATGCTCAAAGA+GGG	+	Chr5:1305206-1305225	MsG0580024143.01.T01:CDS	35.0%
ATGCTGAATACTCTTGTTGA+TGG	+	Chr5:1304942-1304961	MsG0580024143.01.T01:CDS	35.0%
GTACCAAAAGTGAATATGTC+AGG	-	Chr5:1305134-1305153	None:intergenic	35.0%
TATCGAGAACCCATCAATTA+GGG	-	Chr5:1305156-1305175	None:intergenic	35.0%
ATGGGGAAATTGTGTTTGCT+AGG	-	Chr5:1305482-1305501	None:intergenic	40.0%
CATCAACATGATGCTCAAAG+AGG	+	Chr5:1305205-1305224	MsG0580024143.01.T01:CDS	40.0%
CTATCGAGAACCCATCAATT+AGG	-	Chr5:1305157-1305176	None:intergenic	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	1304942	1305541	1304942	ID=MsG0580024143.01;Name=MsG0580024143.01
Chr5	mRNA	1304942	1305541	1304942	ID=MsG0580024143.01.T01;Parent=MsG0580024143.01;Name=MsG0580024143.01.T01;_AED=0.47;_eAED=0.47;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|199
Chr5	exon	1304942	1305541	1304942	ID=MsG0580024143.01.T01:exon:363;Parent=MsG0580024143.01.T01
Chr5	CDS	1304942	1305541	1304942	ID=MsG0580024143.01.T01:cds;Parent=MsG0580024143.01.T01

Gene Sequence

>MsG0580024143.01.T01

ATGCTGAATACTCTTGTTGATGGATTATGCAAGGCTGGTAGTGTTAATGATGCATATGTATTGTATGTTACCATGAGTGAAAAGCATCTTATTCATGGAGATGCATACACTTTTAACTCTCTAATCCATGGATATTGCCTAATTCATAAGATGGACACAACAATTGAAGTGTTCAAGATGATGTCATCGCCTGACATATTCACTTTTGGTACCCTAATTGATGGGTTCTCGATAGAGGGCAGAATCATGGAGAATAAAATTGTCATCAACATGATGCTCAAAGAGGGGTTTAAGTTGGATTTACTTGGCTATACCACTTTGATCAATGCTTACTTCACTGTTAGTAATGTGGATAAAGCAATGAGAATATTTCAGTCGTTGAGACAAAGAAAGATATATTGTGATACTCAGCTATATAATGTAATGTTAAATGGTTTGTGCAAGGCCAATTTGATTGGTGAGGCTCTGCAGCTTTTGGAAGAAGTGCCTCGTGCTGACTTAGTATACTATAGCACTGTAATTGTGTTTGCTAGGATTCCTAGCAAACACAATTTCCCCATTCCTGCAACATCCATCAGTTACAGTGCTATAGGATACTAA

Protein sequence

>MsG0580024143.01.T01

MLNTLVDGLCKAGSVNDAYVLYVTMSEKHLIHGDAYTFNSLIHGYCLIHKMDTTIEVFKMMSSPDIFTFGTLIDGFSIEGRIMENKIVINMMLKEGFKLDLLGYTTLINAYFTVSNVDKAMRIFQSLRQRKIYCDTQLYNVMLNGLCKANLIGEALQLLEEVPRADLVYYSTVIVFARIPSKHNFPIPATSISYSAIGY*