AlfalfaGEDB | MsG0580024260.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024260.01

KEGG: K00660
GO: GO:0003824 GO:0008152 GO:0009058 GO:0016746
PFAM: PF00195 PF02797

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024260.01.T01	XP_003610852.1	93.252	326	4	1	1	308	64	389	0	619

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024260.01.T01	P30075	92.638	326	6	1	1	308	64	389	0	613

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024260.01.T01	G7K6H2	93.252	326	4	1	1	308	64	389	0.0	619

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047831.01	MsG0580024260.01	0.816978	4.185345e-52	3.010037e-49
MsG0080048345.01	MsG0580024260.01	0.858562	7.565960e-63	1.938854e-59
MsG0080048347.01	MsG0580024260.01	0.867009	1.876578e-65	6.452369e-62
MsG0080048348.01	MsG0580024260.01	0.876423	1.412823e-68	6.846458e-65
MsG0080048349.01	MsG0580024260.01	0.869173	3.776678e-66	1.403844e-62
MsG0080048350.01	MsG0580024260.01	0.876679	1.152577e-68	5.638373e-65
MsG0080048352.01	MsG0580024260.01	0.874897	4.716522e-68	2.160123e-64
MsG0080048889.01	MsG0580024260.01	0.825600	4.290885e-54	3.929268e-51
MsG0080049066.01	MsG0580024260.01	-0.812055	5.138746e-51	3.233533e-48
MsG0180000337.01	MsG0580024260.01	0.823679	1.216307e-53	1.054570e-50

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	bit score
MsG0580024260.01.T01	MTR_5g007730	93.252	326	4	1	1	308	64	389	619
MsG0580024260.01.T01	MTR_5g007760	91.718	326	9	1	1	308	64	389	610
MsG0580024260.01.T01	MTR_1g098140	92.025	326	8	1	1	308	64	389	610
MsG0580024260.01.T01	MTR_1g097935	91.718	326	9	1	1	308	64	389	607
MsG0580024260.01.T01	MTR_7g016820	91.718	326	9	1	1	308	64	389	607
MsG0580024260.01.T01	MTR_1g097910	91.411	326	10	1	1	308	64	389	606
MsG0580024260.01.T01	MTR_7g016800	91.411	326	10	1	1	308	64	389	605
MsG0580024260.01.T01	MTR_7g016720	91.718	326	9	1	1	308	64	389	605
MsG0580024260.01.T01	MTR_7g016700	91.411	326	10	1	1	308	64	389	605
MsG0580024260.01.T01	MTR_7g016780	90.798	326	12	1	1	308	64	389	602

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024260.01.T01	AT5G13930	79.448	326	48	1	2	308	70	395	0.0	538
MsG0580024260.01.T01	AT4G00040	40.000	325	170	4	2	306	66	385	1.68e-77	241
MsG0580024260.01.T01	AT4G34850	37.615	327	183	3	2	308	67	392	2.04e-74	234
MsG0580024260.01.T01	AT4G34850	37.500	320	179	3	9	308	1	319	2.33e-72	226
MsG0580024260.01.T01	AT1G02050	39.385	325	172	4	2	306	73	392	8.68e-71	224

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Find 76 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAATGCTCCTGATAGTGA+AGG	0.635736	5:+2695928	MsG0580024260.01.T01:CDS
AACAGGAGAAGGACATGAAT+GGG	0.444037	5:+2696275	MsG0580024260.01.T01:CDS
AAGCGGTGTAGATATGCCTG+GGG	0.707764	5:+2695592	MsG0580024260.01.T01:CDS
AAGTGAGTAATCTTTGACTT+TGG	0.381814	5:-2695554	None:intergenic
AATCAGATATTCCCAATGGT+TGG	0.520494	5:-2696056	None:intergenic
ACAAGCACTATTTGGAGATG+GGG	0.614657	5:+2695829	MsG0580024260.01.T01:CDS
ACAAGCGGTGTAGATATGCC+TGG	0.526709	5:+2695590	MsG0580024260.01.T01:CDS
ACAGGAGAAGGACATGAATG+GGG	0.714424	5:+2696276	MsG0580024260.01.T01:CDS
ACAGTCTCAATGGTAAGTCC+TGG	0.565547	5:-2696318	None:intergenic
ACCTTCTTAAAGATGTTCCT+GGG	0.484500	5:+2695992	MsG0580024260.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AGAAAGAAATCAACTCAAGA+TGG	+	Chr5:2696243-2696262	MsG0580024260.01.T01:CDS	30.0%
CAGTGAAAGCTATAAAAGAA+TGG	+	Chr5:2695525-2695544	MsG0580024260.01.T01:CDS	30.0%
CCATGTATTCACAAACATTA+GGG	-	Chr5:2695439-2695458	None:intergenic	30.0%
GTCCAAATCATCTCAAATAT+AGG	-	Chr5:2695902-2695921	None:intergenic	30.0%
GTGAAAGCTATAAAAGAATG+GGG	+	Chr5:2695527-2695546	MsG0580024260.01.T01:CDS	30.0%
TATCAACTCACTAAACTCTT+AGG	+	Chr5:2695620-2695639	MsG0580024260.01.T01:CDS	30.0%
TGCATGTGTATTGTTTATCT+TGG	+	Chr5:2696212-2696231	MsG0580024260.01.T01:CDS	30.0%
TTGTAATCAGATATTCCCAA+TGG	-	Chr5:2696063-2696082	None:intergenic	30.0%
AATCAGATATTCCCAATGGT+TGG	-	Chr5:2696059-2696078	None:intergenic	35.0%
ACCTTCTTAAAGATGTTCCT+GGG	+	Chr5:2695992-2696011	MsG0580024260.01.T01:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	2695386	2696365	2695386	ID=MsG0580024260.01;Name=MsG0580024260.01
Chr5	mRNA	2695386	2696365	2695386	ID=MsG0580024260.01.T01;Parent=MsG0580024260.01;Name=MsG0580024260.01.T01;_AED=0.48;_eAED=0.49;_QI=0\|0\|0\|1\|1\|1\|2\|0\|308
Chr5	exon	2695386	2695870	2695386	ID=MsG0580024260.01.T01:exon:1811;Parent=MsG0580024260.01.T01
Chr5	exon	2695924	2696365	2695924	ID=MsG0580024260.01.T01:exon:1812;Parent=MsG0580024260.01.T01
Chr5	CDS	2695386	2695870	2695386	ID=MsG0580024260.01.T01:cds;Parent=MsG0580024260.01.T01
Chr5	CDS	2695924	2696365	2695924	ID=MsG0580024260.01.T01:cds;Parent=MsG0580024260.01.T01

Gene Sequence

>MsG0580024260.01.T01

ATGATCAAGAGGAGATACATGTATCTAACAGAGGAGATTTTAAAAGAGAACCCTAATGTTTGTGAATACATGGCACCTTCATTGGATGCTAGACAAGACATGGTGGTGGTAGAAGTACCTAGACTAGGGAAGGAGGCTGCAGTGAAAGCTATAAAAGAATGGGGTCAACCAAAGTCAAAGATTACTCACTTAATCGTTTGCACCACAAGCGGTGTAGATATGCCTGGGGCTGATTATCAACTCACTAAACTCTTAGGTCTTCGTCCATATGTAAAAAGATACATGATGTACCAACAAGGTTGTTTTGCAGGTGGCACGGTACTTCGTTTGGCTAAAGATTTGGCTGAGAACAATAAAGGAGCTCGTGTGCTAGTTGTTTGTTCTGAAGTCACTGCAGTCACATTTCGTGGTCCTAGTGATACTCACTTGGACAGTCTTGTTGGACAAGCACTATTTGGAGATGGGGCAGCTGCACTTATTGTTGGCACAAACAATGCTCCTGATAGTGAAGGTGCCATTGATGGTCACCTTCGTGAAGCTGGATTAACATTTCACCTTCTTAAAGATGTTCCTGGGATTGTGTCAAAAAATATAAATAAAGCATTGGTTGAGGCTTTCCAACCATTGGGAATATCTGATTACAACTCAATCTTTTGGATTGCACACCCTGGTGGTCCTGCAATTCTAGACCAAGTAGAGCAAAAGTTAGCCTTGAAGCCCGAAAAGATGAGAGCCACGAGGGAAGTGCTAAGTGAATATGGAAATATGTCAAGTGCATGTGTATTGTTTATCTTGGATGAAATGAGAAAGAAATCAACTCAAGATGGTTTGAAGACAACAGGAGAAGGACATGAATGGGGTGTGCTATTCGGTTTTGGACCAGGACTTACCATTGAGACTGTTGTCTTGCGCAGTGTGGCTATATGA

Protein sequence

>MsG0580024260.01.T01

MIKRRYMYLTEEILKENPNVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIVCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQALFGDGAAALIVGTNNAPDSEGAIDGHLREAGLTFHLLKDVPGIVSKNINKALVEAFQPLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMRATREVLSEYGNMSSACVLFILDEMRKKSTQDGLKTTGEGHEWGVLFGFGPGLTIETVVLRSVAI*