AlfalfaGEDB | MsG0580025355.01

M. sativa cultivar ZhongmuNo.1 / MsG0580025355.01

KEGG: K01358
GO: GO:0004252 GO:0006508
PFAM: PF00574

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025355.01.T01	XP_003612602.1	83.18	327	18	4	1	320	1	297	0	518

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025355.01.T01	Q94B60	73.577	246	41	2	79	320	67	292	3.45E-117	342

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080047967.01	MsG0580025355.01	0.814622	1.402908e-51	9.460162e-49
MsG0080048036.01	MsG0580025355.01	-0.835360	1.755745e-56	2.142200e-53
MsG0080048059.01	MsG0580025355.01	0.897366	1.508236e-76	1.698259e-72
MsG0080048232.01	MsG0580025355.01	0.804736	1.871769e-49	9.726226e-47
MsG0080048676.01	MsG0580025355.01	0.815103	1.097395e-51	7.496287e-49
MsG0080048776.01	MsG0580025355.01	0.874131	8.580809e-68	3.818141e-64
MsG0080048800.01	MsG0580025355.01	0.828996	6.589274e-55	6.656181e-52
MsG0080048887.01	MsG0580025355.01	0.827886	1.220969e-54	1.194205e-51
MsG0080048896.01	MsG0580025355.01	0.825069	5.731803e-54	5.169997e-51
MsG0080048900.01	MsG0580025355.01	0.829655	4.559595e-55	4.696409e-52

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025355.01.T01	MTR_5g026840	83.180	327	18	4	1	320	1	297	0.0	518
MsG0580025355.01.T01	MTR_7g082980	46.502	243	98	4	41	283	41	251	8.94e-67	211
MsG0580025355.01.T01	MTR_7g109610	43.541	209	96	2	96	302	113	301	1.38e-51	172
MsG0580025355.01.T01	MTR_1g086940	44.019	209	95	2	96	302	116	304	3.64e-51	171
MsG0580025355.01.T01	MTR_4g075100	37.799	209	109	2	78	285	31	219	4.67e-42	146
MsG0580025355.01.T01	MTR_4g069800	37.019	208	111	1	78	285	86	273	7.76e-42	146
MsG0580025355.01.T01	MTR_4g069800	37.056	197	104	1	89	285	65	241	2.94e-40	141
MsG0580025355.01.T01	MTR_4g034690	34.112	214	114	5	69	281	6	193	5.87e-35	126
MsG0580025355.01.T01	MTR_4g102000	31.064	235	135	3	55	282	87	301	4.70e-32	122
MsG0580025355.01.T01	MTR_0002s0730	34.579	214	115	4	78	287	6	198	1.12e-30	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580025355.01.T01	AT5G45390	73.577	246	41	2	79	320	67	292	3.52e-118	342
MsG0580025355.01.T01	AT1G66670	46.635	208	91	1	76	283	70	257	1.58e-59	193
MsG0580025355.01.T01	AT1G02560	46.524	187	80	1	96	282	119	285	1.20e-52	175
MsG0580025355.01.T01	AT1G11750	37.500	208	110	1	78	285	101	288	7.63e-43	149
MsG0580025355.01.T01	AT1G11750	37.500	208	110	1	78	285	83	270	8.62e-43	149
MsG0580025355.01.T01	AT5G23140	37.198	207	109	2	78	283	34	220	3.08e-42	146
MsG0580025355.01.T01	ATCG00670	37.500	192	99	2	91	281	22	193	5.94e-36	129
MsG0580025355.01.T01	AT4G17040	37.949	195	109	2	91	285	105	287	1.04e-35	131
MsG0580025355.01.T01	AT1G12410	33.333	192	108	1	91	282	89	260	8.32e-34	125
MsG0580025355.01.T01	AT1G49970	35.323	201	118	2	86	285	166	355	1.16e-29	117

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Find 112 sgRNAs with CRISPR-Local

Find 418 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCTTGATATCTTTAGT+AGG	0.425124	5:-17381204	None:intergenic
AAAGACAAATAAAACTGCCA+CGG	0.646339	5:-17386144	None:intergenic
AACTTGTTCAAGTGAGCGGC+CGG	0.548501	5:-17385785	None:intergenic
AAGCACCACAACTCTCACAA+TGG	0.683599	5:+17380804	MsG0580025355.01.T01:exon
AAGTTCTGAAAGATATCGAT+AGG	0.559233	5:+17385803	MsG0580025355.01.T01:CDS
AATGTCTTCGCCACAGACTC+CGG	0.530994	5:+17381044	MsG0580025355.01.T01:CDS
ACAGCCTCAATTATCCTAGG+TGG	0.682148	5:+17385607	MsG0580025355.01.T01:CDS
ACGATGCGGTGCAGCTTGTT+AGG	0.301821	5:+17385545	MsG0580025355.01.T01:CDS
ACTCCGGAACGGGCTATGAG+AGG	0.646659	5:+17381060	MsG0580025355.01.T01:CDS
ACTTAAGATTCATCCCCTTG+CGG	0.632344	5:+17386038	MsG0580025355.01.T01:three_prime_UTR

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCAAGGTCATATTGAA+AGG	+	Chr5:17384451-17384470	MsG0580025355.01.T01:intron	30.0%
AAACCAAGGTCATATTGAAA+GGG	+	Chr5:17384452-17384471	MsG0580025355.01.T01:intron	30.0%
AAAGACAAATAAAACTGCCA+CGG	-	Chr5:17386147-17386166	None:intergenic	30.0%
AAAGGATTATGCATTTAGTC+TGG	+	Chr5:17382991-17383010	MsG0580025355.01.T01:intron	30.0%
AACTAAACAGATGACTGTTT+AGG	+	Chr5:17385344-17385363	MsG0580025355.01.T01:intron	30.0%
AATAATTACTAGCAGTAGCA+TGG	-	Chr5:17383601-17383620	None:intergenic	30.0%
AATGTGTAAAATATGTGCCT+TGG	+	Chr5:17381299-17381318	MsG0580025355.01.T01:intron	30.0%
AATTCGCCAAAAAAGATTCA+TGG	+	Chr5:17384991-17385010	MsG0580025355.01.T01:intron	30.0%
ACAAAAAGACAGAACGAAAA+TGG	-	Chr5:17383674-17383693	None:intergenic	30.0%
ACCATCCTTAACACTATTAT+TGG	-	Chr5:17383122-17383141	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	17380755	17386232	17380755	ID=MsG0580025355.01;Name=MsG0580025355.01
Chr5	mRNA	17380755	17386232	17380755	ID=MsG0580025355.01.T01;Parent=MsG0580025355.01;Name=MsG0580025355.01.T01;_AED=0.44;_eAED=0.46;_QI=68\|0\|0.66\|1\|1\|1\|3\|233\|320
Chr5	exon	17380755	17381253	17380755	ID=MsG0580025355.01.T01:exon:30289;Parent=MsG0580025355.01.T01
Chr5	exon	17381334	17381393	17381334	ID=MsG0580025355.01.T01:exon:30290;Parent=MsG0580025355.01.T01
Chr5	exon	17385528	17386232	17385528	ID=MsG0580025355.01.T01:exon:30291;Parent=MsG0580025355.01.T01
Chr5	five_prime_UTR	17380755	17380822	17380755	ID=MsG0580025355.01.T01:five_prime_utr;Parent=MsG0580025355.01.T01
Chr5	CDS	17380823	17381253	17380823	ID=MsG0580025355.01.T01:cds;Parent=MsG0580025355.01.T01
Chr5	CDS	17381334	17381393	17381334	ID=MsG0580025355.01.T01:cds;Parent=MsG0580025355.01.T01
Chr5	CDS	17385528	17385999	17385528	ID=MsG0580025355.01.T01:cds;Parent=MsG0580025355.01.T01
Chr5	three_prime_UTR	17386000	17386232	17386000	ID=MsG0580025355.01.T01:three_prime_utr;Parent=MsG0580025355.01.T01

Gene Sequence

>MsG0580025355.01.T01

ATGGATTTACTCACAACCTCTCTTTCTCATCCTTCAACTTCCCTTCCATCTCTCTCACAACGCATTCCCACCCTCTTTTCAAAACCACCCATTTCCTCTTTCAACCCTAAACCACAATTTCGCACAACTCCAATCAACTGCGTCCTCACAACTCCATCAACAAAACCCTCCAAAGATTCAAACTTTAACCCCCAACTTCAAAAACCCACTATCACCTTTCAAATGTCTTCGCCACAGACTCCGGAACGGGCTATGAGAGGTGCTGAATCTGATACTATGGGGTTGTTGTTGAGGGAGAGGATTGTGTTCTTGGGTAGTGAAATTGATGATTTTGTTGCTGATGCTATTATGAGTCAGTTGTTGCTTTTGGATGCTCAAGATCCTACTAAAGATATCAAGCTTTTTATCAATTCTCCCGGTGGTTCTCTCAGGTTTATGAGAATTATTGGAATTGTATGTGATAAGGTAGATTGGGAAGATAAGACTAAAGGTGCTACCATGGCTATCTACGATGCGGTGCAGCTTGTTAGGGCGGATGTATCCACAATTGCAATGGGCATTGCAGCATCAACAGCCTCAATTATCCTAGGTGGTGGCACCAAAGGTAAGCGGCTAGCTATGCCCAATACGCGAATCATGATTGCTCAACCACCTGGAGGTGCTAGTGGACAAGCTATAGATGTAGAAATTCAAGCTAAAGAACTCATGCACAACAAGAATAATGTGGTCAGAATCATATCTTCCTTCACCGGCCGCTCACTTGAACAAGTTCTGAAAGATATCGATAGGGATAGATATCTGTCTCCAATTGAAGCGGTGGAATATGGAATTATTGACGGGGTAATCGATAGAGAAATGATTATTCCTCTCCTGCCAGTGCCAGAAAAGGTGGAAGAAGCAACACTAAACACCGATGATCCGAATTTCCTAACCCCACATATCCCAGATGATGAGATATACTAG

Protein sequence

>MsG0580025355.01.T01

MDLLTTSLSHPSTSLPSLSQRIPTLFSKPPISSFNPKPQFRTTPINCVLTTPSTKPSKDSNFNPQLQKPTITFQMSSPQTPERAMRGAESDTMGLLLRERIVFLGSEIDDFVADAIMSQLLLLDAQDPTKDIKLFINSPGGSLRFMRIIGIVCDKVDWEDKTKGATMAIYDAVQLVRADVSTIAMGIAASTASIILGGGTKGKRLAMPNTRIMIAQPPGGASGQAIDVEIQAKELMHNKNNVVRIISSFTGRSLEQVLKDIDRDRYLSPIEAVEYGIIDGVIDREMIIPLLPVPEKVEEATLNTDDPNFLTPHIPDDEIY*