AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048676.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048676.01.T01 MTR_4g006200 95.873 315 13 0 1 315 1 315 0.0 631
MsG0080048676.01.T01 MTR_3g006055 58.235 170 23 3 153 315 1 129 4.24e-55 176
MsG0080048676.01.T01 MTR_4g005800 88.158 76 9 0 190 265 48 123 1.55e-44 148
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048676.01.T01 AT3G21200 54.517 321 122 8 1 313 1 305 1.05e-118 344
MsG0080048676.01.T01 AT3G03890 28.295 258 153 12 53 292 75 318 2.20e-12 67.0
MsG0080048676.01.T01 AT3G03890 28.163 245 144 12 53 279 75 305 2.13e-11 63.9

Find 83 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTATTCACTCCTCCGTAAA+TGG 0.157465 contig399end:+63335 MsG0080048676.01.T01:five_prime_UTR
TGAAGTTTCAAGAACAATTA+TGG 0.206636 contig399end:+63695 MsG0080048676.01.T01:CDS
CCTTGCATATTTGATGAAAT+TGG 0.214626 contig399end:-66229 None:intergenic
AGTAATGTCTTCCATATTAT+TGG 0.225630 contig399end:-65591 None:intergenic
CGATTGTTGATTGTTCTAAT+TGG 0.226764 contig399end:-63644 None:intergenic
TGAAGGGTGTGTTGAGATTT+TGG 0.236408 contig399end:-63574 None:intergenic
CTATGTTGATTTGGATTTCC+AGG 0.294434 contig399end:+65628 MsG0080048676.01.T01:CDS
TGTTGAAATGAAAGGGAAAA+TGG 0.302674 contig399end:-63410 None:intergenic
TGAAATTAACACCAATAATA+TGG 0.307435 contig399end:+65580 MsG0080048676.01.T01:CDS
TCACAGAAGCAAAGATGATA+TGG 0.313832 contig399end:+65965 MsG0080048676.01.T01:CDS
TGTATATTATTGCTGTAGAT+AGG 0.315510 contig399end:+65306 MsG0080048676.01.T01:CDS
GCTGCACTTGGATGACTTCC+AGG 0.317388 contig399end:+65331 MsG0080048676.01.T01:CDS
TTCGTTATTGATTCTGAAGA+TGG 0.354749 contig399end:+63786 MsG0080048676.01.T01:CDS
ATGGGTAGACCGTTTGGGTT+TGG 0.369113 contig399end:+65984 MsG0080048676.01.T01:CDS
TTGTAATGTCTATGTTGATT+TGG 0.370501 contig399end:+65619 MsG0080048676.01.T01:CDS
ACTTTCAACGTTAACCCAAC+AGG 0.382511 contig399end:+63737 MsG0080048676.01.T01:CDS
ATTTAACTGTATGTCACAAT+TGG 0.386834 contig399end:+66098 MsG0080048676.01.T01:CDS
AGAGAAGTCACAGATGAAAA+AGG 0.391076 contig399end:+66063 MsG0080048676.01.T01:CDS
CACAGCGGCTTGTTTCTTTG+TGG 0.392329 contig399end:+65246 MsG0080048676.01.T01:intron
GATGATATGGGTAGACCGTT+TGG 0.397685 contig399end:+65978 MsG0080048676.01.T01:CDS
GATTGTTGATTGTTCTAATT+GGG 0.406772 contig399end:-63643 None:intergenic
ATGATATGGGTAGACCGTTT+GGG 0.415103 contig399end:+65979 MsG0080048676.01.T01:CDS
TCCTAACAACGGTTTATTTG+AGG 0.427212 contig399end:+66023 MsG0080048676.01.T01:CDS
GAACAATTATGGATTTAGCT+AGG 0.431943 contig399end:+63706 MsG0080048676.01.T01:CDS
TCTTTGTGGAAGAAGAGGTT+TGG 0.434962 contig399end:+65260 MsG0080048676.01.T01:CDS
ACTGTATGTCACAATTGGCT+TGG 0.444223 contig399end:+66103 MsG0080048676.01.T01:CDS
CGAAATGAGATTGAATTTGA+AGG 0.447078 contig399end:-63591 None:intergenic
ACCCAACAGGGTTATCCTCT+TGG 0.450020 contig399end:+63750 MsG0080048676.01.T01:CDS
CTGTATGTCACAATTGGCTT+GGG 0.450082 contig399end:+66104 MsG0080048676.01.T01:CDS
ATTCTGTGATGATTCATTTG+AGG 0.453038 contig399end:+66300 MsG0080048676.01.T01:three_prime_UTR
AGAAAGAAACTTCCATCCTC+TGG 0.456228 contig399end:+66131 MsG0080048676.01.T01:CDS
TGTTCAGTGTTGAAATGAAA+GGG 0.456955 contig399end:-63417 None:intergenic
AACAATTATGGATTTAGCTA+GGG 0.462844 contig399end:+63707 MsG0080048676.01.T01:CDS
CTTGAAACTTCAGATGGAAA+CGG 0.466933 contig399end:-63684 None:intergenic
ACTCCTCAGTGTACACTTCA+AGG 0.467071 contig399end:+64024 MsG0080048676.01.T01:CDS
TAAATGGGCAAATCCAAAAT+CGG 0.468532 contig399end:+63351 MsG0080048676.01.T01:five_prime_UTR
TGTAGATAGGGTGCTGCACT+TGG 0.473744 contig399end:+65319 MsG0080048676.01.T01:CDS
TTATTCACTCCTCCGTAAAT+GGG 0.477846 contig399end:+63336 MsG0080048676.01.T01:five_prime_UTR
CAGGGTTATCCTCTTGGTGT+TGG 0.489252 contig399end:+63756 MsG0080048676.01.T01:CDS
ATTATGGATTTAGCTAGGGT+TGG 0.490791 contig399end:+63711 MsG0080048676.01.T01:CDS
ACCTCAAATAAACCGTTGTT+AGG 0.493427 contig399end:-66024 None:intergenic
ATTGTTCTTGAAACTTCAGA+TGG 0.496689 contig399end:-63690 None:intergenic
GAAATGAGATTGAATTTGAA+GGG 0.509653 contig399end:-63590 None:intergenic
TAACTCACCTTGATCAAAGC+TGG 0.516382 contig399end:-64069 None:intergenic
TTGTTCAGTGTTGAAATGAA+AGG 0.517988 contig399end:-63418 None:intergenic
GTATATTATTGCTGTAGATA+GGG 0.518730 contig399end:+65307 MsG0080048676.01.T01:CDS
GAAGATTGAATTGAAATTGA+AGG 0.519184 contig399end:-63477 None:intergenic
TTTCATCTGTGACTTCTCTA+GGG 0.522593 contig399end:-66059 None:intergenic
ACCAAGAGGATAACCCTGTT+GGG 0.525860 contig399end:-63751 None:intergenic
GTTGTTGGTGTAAAACATGA+TGG 0.527632 contig399end:-63540 None:intergenic
CATACAGTTAAATGTTGACT+TGG 0.529692 contig399end:-66088 None:intergenic
ACCTTGACATGAAGTGAAGA+GGG 0.536966 contig399end:-63870 None:intergenic
CACCAAGAGGATAACCCTGT+TGG 0.548682 contig399end:-63752 None:intergenic
CCTTCTCAAAATCCAGAGGA+TGG 0.550636 contig399end:-66143 None:intergenic
TTGTTTCTTTGTGGAAGAAG+AGG 0.550656 contig399end:+65255 MsG0080048676.01.T01:CDS
ATATTTGATGAAATTGGTAG+TGG 0.551633 contig399end:-66223 None:intergenic
CACAGAAGCAAAGATGATAT+GGG 0.552316 contig399end:+65966 MsG0080048676.01.T01:CDS
AACGCATATCCAAACCCAAA+CGG 0.555598 contig399end:-65993 None:intergenic
AATTCTCAGCAAACTCTCGA+AGG 0.558854 contig399end:-65550 None:intergenic
GCAGGATCCAGCTTTGATCA+AGG 0.570718 contig399end:+64062 MsG0080048676.01.T01:CDS
ATTCTCAGCAAACTCTCGAA+GGG 0.571271 contig399end:-65549 None:intergenic
CTTTCAACGTTAACCCAACA+GGG 0.573683 contig399end:+63738 MsG0080048676.01.T01:CDS
GTGAATGTTATTATCAGTGG+TGG 0.576165 contig399end:-63844 None:intergenic
TCTGTGACTTCTCTAGGGAA+AGG 0.588455 contig399end:-66054 None:intergenic
TTGATCAAAGCTGGATCCTG+CGG 0.589973 contig399end:-64060 None:intergenic
TACCTTGACATGAAGTGAAG+AGG 0.592658 contig399end:-63871 None:intergenic
CCCCTCTTCACTTCATGTCA+AGG 0.595937 contig399end:+63869 MsG0080048676.01.T01:CDS
TATGTCACAATTGGCTTGGG+AGG 0.603045 contig399end:+66107 MsG0080048676.01.T01:CDS
GATTGAATTGAAATTGAAGG+TGG 0.604584 contig399end:-63474 None:intergenic
GGTGTGAATGTTATTATCAG+TGG 0.609297 contig399end:-63847 None:intergenic
CCAATTTCATCAAATATGCA+AGG 0.612346 contig399end:+66229 MsG0080048676.01.T01:three_prime_UTR
TTCTCAGCAAACTCTCGAAG+GGG 0.632254 contig399end:-65548 None:intergenic
AGGAACGCTCACGAAACCGC+AGG 0.635837 contig399end:+64044 MsG0080048676.01.T01:CDS
AATCGAACACCAACACCAAG+AGG 0.641303 contig399end:-63765 None:intergenic
CTTCTCTAGGGAAAGGAATG+CGG 0.641717 contig399end:-66047 None:intergenic
TCTCAGCAAACTCTCGAAGG+GGG 0.641945 contig399end:-65547 None:intergenic
TTGGATTTGCCCATTTACGG+AGG 0.655579 contig399end:-63345 None:intergenic
TGAATGTTATTATCAGTGGT+GGG 0.656145 contig399end:-63843 None:intergenic
GTAACCTTCTCAAAATCCAG+AGG 0.672238 contig399end:-66147 None:intergenic
CTTGACATGAAGTGAAGAGG+GGG 0.679817 contig399end:-63868 None:intergenic
CCTTGACATGAAGTGAAGAG+GGG 0.702259 contig399end:-63869 None:intergenic
GCACTTGGATGACTTCCAGG+AGG 0.711976 contig399end:+65334 MsG0080048676.01.T01:CDS
GTTCCTTGAAGTGTACACTG+AGG 0.794003 contig399end:-64027 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTCTATGTAAATAAATC+AGG - contig399end:65746-65765 None:intergenic 15.0%
!! AACTAATGTGATATATTATA+TGG - contig399end:64744-64763 None:intergenic 15.0%
!! ACTAAATTAACTAATTCAAA+AGG + contig399end:64955-64974 MsG0080048676.01.T01:intron 15.0%
!! CTAAATTAACTAATTCAAAA+GGG + contig399end:64956-64975 MsG0080048676.01.T01:intron 15.0%
!! GGATAACAAATAAATAAAAA+TGG - contig399end:65705-65724 None:intergenic 15.0%
!! TATGATTGAAATAGAAAAAA+GGG - contig399end:63815-63834 None:intergenic 15.0%
!! TTATGATTGAAATAGAAAAA+AGG - contig399end:63816-63835 None:intergenic 15.0%
!! TTATTTACATAGAATTTAAC+TGG + contig399end:65749-65768 MsG0080048676.01.T01:intron 15.0%
!!! ATTCTAAAATTAAGTTCAAT+TGG + contig399end:64537-64556 MsG0080048676.01.T01:intron 15.0%
!!! TATTCAAATTTTGAGTTTTT+TGG + contig399end:63892-63911 MsG0080048676.01.T01:intron 15.0%
!!! TGATTGATGATTTTTAATTT+TGG + contig399end:64985-65004 MsG0080048676.01.T01:intron 15.0%
!! TGAAATTAACACCAATAATA+TGG + contig399end:65580-65599 MsG0080048676.01.T01:CDS 20.0%
!!! AGTTGTTTTGTTATATAAGT+TGG + contig399end:65458-65477 MsG0080048676.01.T01:intron 20.0%
!!! AGTTTTTTGGATGAATTAAT+GGG + contig399end:63905-63924 MsG0080048676.01.T01:intron 20.0%
!!! TGATCTTTTAAATGAACAAA+AGG + contig399end:64711-64730 MsG0080048676.01.T01:intron 20.0%
! AACAATTATGGATTTAGCTA+GGG + contig399end:63707-63726 MsG0080048676.01.T01:CDS 25.0%
! AATTTAGTCCCTTAAATTGT+AGG + contig399end:64202-64221 MsG0080048676.01.T01:intron 25.0%
! AATTTATCCCTAGAAATTCT+CGG - contig399end:65036-65055 None:intergenic 25.0%
! ACTAATTTGACTAACTCAAA+AGG + contig399end:64468-64487 MsG0080048676.01.T01:intron 25.0%
! AGCATTCTTATAAAAATCAC+TGG + contig399end:65837-65856 MsG0080048676.01.T01:intron 25.0%
! AGTAATGTCTTCCATATTAT+TGG - contig399end:65594-65613 None:intergenic 25.0%
! ATATAACAAAACAACTTGCA+AGG - contig399end:65454-65473 None:intergenic 25.0%
! ATATTTGATGAAATTGGTAG+TGG - contig399end:66226-66245 None:intergenic 25.0%
! ATTAATGGGATAATTGGAAT+TGG + contig399end:63919-63938 MsG0080048676.01.T01:intron 25.0%
! ATTTAACTGTATGTCACAAT+TGG + contig399end:66098-66117 MsG0080048676.01.T01:CDS 25.0%
! ATTTAGTCCCTTAAATTGTA+GGG + contig399end:64203-64222 MsG0080048676.01.T01:intron 25.0%
! CTAACTTCAAAACAATATTG+AGG + contig399end:65885-65904 MsG0080048676.01.T01:intron 25.0%
! GAAATGAGATTGAATTTGAA+GGG - contig399end:63593-63612 None:intergenic 25.0%
! GAAGATTGAATTGAAATTGA+AGG - contig399end:63480-63499 None:intergenic 25.0%
! GAAGTTAGATTTCTATTTAG+TGG - contig399end:65873-65892 None:intergenic 25.0%
! GAATACAAACACCATAAAAA+TGG - contig399end:66284-66303 None:intergenic 25.0%
! GTATATTATTGCTGTAGATA+GGG + contig399end:65307-65326 MsG0080048676.01.T01:CDS 25.0%
! TGAAGTTTCAAGAACAATTA+TGG + contig399end:63695-63714 MsG0080048676.01.T01:CDS 25.0%
! TGTATATTATTGCTGTAGAT+AGG + contig399end:65306-65325 MsG0080048676.01.T01:CDS 25.0%
! TTAATTACCGAGAATTTCTA+GGG + contig399end:65026-65045 MsG0080048676.01.T01:intron 25.0%
! TTAGTATAGATACATATGCA+TGG + contig399end:64148-64167 MsG0080048676.01.T01:intron 25.0%
! TTGCTGATACTAATTGTAAT+AGG + contig399end:65412-65431 MsG0080048676.01.T01:intron 25.0%
! TTTAATAAAAATGAAGGGTC+AGG - contig399end:65367-65386 None:intergenic 25.0%
!! GATTGTTGATTGTTCTAATT+GGG - contig399end:63646-63665 None:intergenic 25.0%
!! TTGTAATGTCTATGTTGATT+TGG + contig399end:65619-65638 MsG0080048676.01.T01:CDS 25.0%
!! TTGTTATCCTTTGATTTAAC+TGG + contig399end:65716-65735 MsG0080048676.01.T01:intron 25.0%
!!! CAAGAATATGACCATTTTTA+TGG + contig399end:66270-66289 MsG0080048676.01.T01:three_prime_UTR 25.0%
!!! GAGTTTTTTGGATGAATTAA+TGG + contig399end:63904-63923 MsG0080048676.01.T01:intron 25.0%
!!! TTATTTTTGTATCAACCATC+CGG - contig399end:64902-64921 None:intergenic 25.0%
AAAATCACATTAGTTCGTCT+TGG + contig399end:64434-64453 MsG0080048676.01.T01:intron 30.0%
AAACTAATGTGGTCTATTGT+TGG - contig399end:64230-64249 None:intergenic 30.0%
AAAGTGATTGATGTTGACAA+AGG + contig399end:65107-65126 MsG0080048676.01.T01:intron 30.0%
AAATGCTGTGTAGATAAATC+AGG - contig399end:65779-65798 None:intergenic 30.0%
ACACAGCAAAACTAACTTAT+CGG - contig399end:65912-65931 None:intergenic 30.0%
ATTCTGTGATGATTCATTTG+AGG + contig399end:66300-66319 MsG0080048676.01.T01:three_prime_UTR 30.0%
ATTGTTCTTGAAACTTCAGA+TGG - contig399end:63693-63712 None:intergenic 30.0%
CCAATTTCATCAAATATGCA+AGG + contig399end:66229-66248 MsG0080048676.01.T01:three_prime_UTR 30.0%
CCTTGCATATTTGATGAAAT+TGG - contig399end:66232-66251 None:intergenic 30.0%
CGAAATGAGATTGAATTTGA+AGG - contig399end:63594-63613 None:intergenic 30.0%
CGCATTAATTCCAAGAAAAT+GGG - contig399end:64384-64403 None:intergenic 30.0%
GAACAATTATGGATTTAGCT+AGG + contig399end:63706-63725 MsG0080048676.01.T01:CDS 30.0%
GATTGAATTGAAATTGAAGG+TGG - contig399end:63477-63496 None:intergenic 30.0%
GCTATATGAAACTACAATCA+AGG - contig399end:65168-65187 None:intergenic 30.0%
GGATAATTGGAATTGGAATT+GGG + contig399end:63926-63945 MsG0080048676.01.T01:intron 30.0%
GGATGAATTAATGGGATAAT+TGG + contig399end:63913-63932 MsG0080048676.01.T01:intron 30.0%
GTTAATTACCGAGAATTTCT+AGG + contig399end:65025-65044 MsG0080048676.01.T01:intron 30.0%
TAAATGGGCAAATCCAAAAT+CGG + contig399end:63351-63370 MsG0080048676.01.T01:five_prime_UTR 30.0%
TAAGTTCAATTGGACAATGT+TGG + contig399end:64547-64566 MsG0080048676.01.T01:intron 30.0%
TAATCAGTTAGTAACAGTGA+AGG + contig399end:64609-64628 MsG0080048676.01.T01:intron 30.0%
TATCAGCAAACTAGTAGTAT+AGG - contig399end:65401-65420 None:intergenic 30.0%
TCACAGTTTGAAAAAACCTT+AGG - contig399end:64641-64660 None:intergenic 30.0%
TCGCATTAATTCCAAGAAAA+TGG - contig399end:64385-64404 None:intergenic 30.0%
TGAATGTTATTATCAGTGGT+GGG - contig399end:63846-63865 None:intergenic 30.0%
TGGTTAATGAGTTTCAGTTA+AGG + contig399end:64787-64806 MsG0080048676.01.T01:intron 30.0%
TGTTGAAATGAAAGGGAAAA+TGG - contig399end:63413-63432 None:intergenic 30.0%
! AGCATTTTTGTAGTCTAAAG+AGG - contig399end:65519-65538 None:intergenic 30.0%
! ATTGACTCTAAGAGTTAAGA+CGG + contig399end:64404-64423 MsG0080048676.01.T01:intron 30.0%
! ATTTTGGATTTGCCCATTTA+CGG - contig399end:63351-63370 None:intergenic 30.0%
! CATACAGTTAAATGTTGACT+TGG - contig399end:66091-66110 None:intergenic 30.0%
! CGATTGTTGATTGTTCTAAT+TGG - contig399end:63647-63666 None:intergenic 30.0%
! GAGAATTTCTAGGGATAAAT+TGG + contig399end:65035-65054 MsG0080048676.01.T01:intron 30.0%
! TGTATTTGATTTCCCATTGT+AGG + contig399end:65942-65961 MsG0080048676.01.T01:intron 30.0%
! TGTTCAGTGTTGAAATGAAA+GGG - contig399end:63420-63439 None:intergenic 30.0%
! TTAGTTTGAATCAAGTGGTT+TGG + contig399end:64243-64262 MsG0080048676.01.T01:intron 30.0%
! TTGTTCAGTGTTGAAATGAA+AGG - contig399end:63421-63440 None:intergenic 30.0%
!! TTCGTTATTGATTCTGAAGA+TGG + contig399end:63786-63805 MsG0080048676.01.T01:CDS 30.0%
!!! GTTGTGTTTTTTGTTCTTGT+TGG - contig399end:63455-63474 None:intergenic 30.0%
AAACTACAATCAAGGTCACA+AGG - contig399end:65160-65179 None:intergenic 35.0%
AACAATCTTTGGCCAAACTT+TGG + contig399end:64849-64868 MsG0080048676.01.T01:intron 35.0%
ACCTCAAATAAACCGTTGTT+AGG - contig399end:66027-66046 None:intergenic 35.0%
AGAGAAGTCACAGATGAAAA+AGG + contig399end:66063-66082 MsG0080048676.01.T01:CDS 35.0%
AGAGTTGAGGAGAGTAAAAT+TGG - contig399end:64349-64368 None:intergenic 35.0%
ATTATGGATTTAGCTAGGGT+TGG + contig399end:63711-63730 MsG0080048676.01.T01:CDS 35.0%
CAAAAGGACTAATGTGATTG+AGG + contig399end:64484-64503 MsG0080048676.01.T01:intron 35.0%
CACAGAAGCAAAGATGATAT+GGG + contig399end:65966-65985 MsG0080048676.01.T01:CDS 35.0%
CCACTTGATTCAAACTAATG+TGG - contig399end:64241-64260 None:intergenic 35.0%
CTTGAAACTTCAGATGGAAA+CGG - contig399end:63687-63706 None:intergenic 35.0%
GGGATAATTGGAATTGGAAT+TGG + contig399end:63925-63944 MsG0080048676.01.T01:intron 35.0%
GGTGTGAATGTTATTATCAG+TGG - contig399end:63850-63869 None:intergenic 35.0%
GTGAATGTTATTATCAGTGG+TGG - contig399end:63847-63866 None:intergenic 35.0%
GTTGTTGGTGTAAAACATGA+TGG - contig399end:63543-63562 None:intergenic 35.0%
TCACAGAAGCAAAGATGATA+TGG + contig399end:65965-65984 MsG0080048676.01.T01:CDS 35.0%
TCCTAACAACGGTTTATTTG+AGG + contig399end:66023-66042 MsG0080048676.01.T01:CDS 35.0%
TGTTGGACCCTACAATTTAA+GGG - contig399end:64213-64232 None:intergenic 35.0%
TTATTCACTCCTCCGTAAAT+GGG + contig399end:63336-63355 MsG0080048676.01.T01:five_prime_UTR 35.0%
TTCTTGGCTAGAACAATTTG+TGG + contig399end:64323-64342 MsG0080048676.01.T01:intron 35.0%
TTGTTGGACCCTACAATTTA+AGG - contig399end:64214-64233 None:intergenic 35.0%
TTTAGTCTCTACAATTGTCC+AGG - contig399end:64941-64960 None:intergenic 35.0%
TTTCATCTGTGACTTCTCTA+GGG - contig399end:66062-66081 None:intergenic 35.0%
! AATTCCAAGAAAATGGGTTC+TGG - contig399end:64378-64397 None:intergenic 35.0%
! AATTTTACTCTCCTCAACTC+TGG + contig399end:64348-64367 MsG0080048676.01.T01:intron 35.0%
! ACATGTGTCTTTTCCGATTT+TGG - contig399end:63367-63386 None:intergenic 35.0%
! ATTACCAGAACCCATTTTCT+TGG + contig399end:64371-64390 MsG0080048676.01.T01:intron 35.0%
! CCACATTAGTTTGAATCAAG+TGG + contig399end:64238-64257 MsG0080048676.01.T01:intron 35.0%
! TTGACTCTAAGAGTTAAGAC+GGG + contig399end:64405-64424 MsG0080048676.01.T01:intron 35.0%
! TTGTTTCTTTGTGGAAGAAG+AGG + contig399end:65255-65274 MsG0080048676.01.T01:CDS 35.0%
! TTTTCATCTGTGACTTCTCT+AGG - contig399end:66063-66082 None:intergenic 35.0%
!! CGTTTGTTTTCTCCTAACAA+CGG + contig399end:66012-66031 MsG0080048676.01.T01:CDS 35.0%
!! CTATGTTGATTTGGATTTCC+AGG + contig399end:65628-65647 MsG0080048676.01.T01:CDS 35.0%
!!! CTTGTTTTGCAAGGCATTTT+TGG + contig399end:66353-66372 MsG0080048676.01.T01:three_prime_UTR 35.0%
!!! TATGTCCTTGTTTTTCAGGA+CGG + contig399end:65482-65501 MsG0080048676.01.T01:intron 35.0%
!!! TGTGTTGAGATTTTGGTGTT+TGG - contig399end:63570-63589 None:intergenic 35.0%
!!! TTGGTGTTTGGTTTTGTTGT+TGG - contig399end:63558-63577 None:intergenic 35.0%
AACAAGAAGCTCAACTCAAG+TGG + contig399end:64767-64786 MsG0080048676.01.T01:intron 40.0%
AACATTGCATTAGTTCGTCC+TGG + contig399end:64920-64939 MsG0080048676.01.T01:intron 40.0%
AACGCATATCCAAACCCAAA+CGG - contig399end:65996-66015 None:intergenic 40.0%
AATTCTCAGCAAACTCTCGA+AGG - contig399end:65553-65572 None:intergenic 40.0%
ACAATCAAGGTCACAAGGTA+AGG - contig399end:65155-65174 None:intergenic 40.0%
ACCTTGACATGAAGTGAAGA+GGG - contig399end:63873-63892 None:intergenic 40.0%
ACTGTATGTCACAATTGGCT+TGG + contig399end:66103-66122 MsG0080048676.01.T01:CDS 40.0%
ACTTTCAACGTTAACCCAAC+AGG + contig399end:63737-63756 MsG0080048676.01.T01:CDS 40.0%
AGAAAGAAACTTCCATCCTC+TGG + contig399end:66131-66150 MsG0080048676.01.T01:CDS 40.0%
AGGTGCACCAGTTAAATCAA+AGG - contig399end:65726-65745 None:intergenic 40.0%
AGTTTAGCCGCTGAAATTGT+AGG + contig399end:64676-64695 MsG0080048676.01.T01:intron 40.0%
ATGATATGGGTAGACCGTTT+GGG + contig399end:65979-65998 MsG0080048676.01.T01:CDS 40.0%
ATTCTCAGCAAACTCTCGAA+GGG - contig399end:65552-65571 None:intergenic 40.0%
CAATCAAGGTCACAAGGTAA+GGG - contig399end:65154-65173 None:intergenic 40.0%
CATGCTATGTAGATACATGC+AGG - contig399end:65661-65680 None:intergenic 40.0%
CTTATTCACTCCTCCGTAAA+TGG + contig399end:63335-63354 MsG0080048676.01.T01:five_prime_UTR 40.0%
CTTTCAACGTTAACCCAACA+GGG + contig399end:63738-63757 MsG0080048676.01.T01:CDS 40.0%
CTTTGATCAAGGTGAGTTAG+TGG + contig399end:64073-64092 MsG0080048676.01.T01:intron 40.0%
GAGTACCTGAGTTTGATTCT+TGG + contig399end:64307-64326 MsG0080048676.01.T01:intron 40.0%
GTAACCTTCTCAAAATCCAG+AGG - contig399end:66150-66169 None:intergenic 40.0%
GTGATTGAGGATCTCAATGT+TGG + contig399end:64497-64516 MsG0080048676.01.T01:intron 40.0%
GTGATTGATGTTGACAAAGG+TGG + contig399end:65110-65129 MsG0080048676.01.T01:intron 40.0%
TAACTCACCTTGATCAAAGC+TGG - contig399end:64072-64091 None:intergenic 40.0%
TACCTTGACATGAAGTGAAG+AGG - contig399end:63874-63893 None:intergenic 40.0%
TCTAGCCAAGAATCAAACTC+AGG - contig399end:64315-64334 None:intergenic 40.0%
TCTTTGTGGAAGAAGAGGTT+TGG + contig399end:65260-65279 MsG0080048676.01.T01:CDS 40.0%
TGCATAAATACAGTCACAGC+AGG - contig399end:65818-65837 None:intergenic 40.0%
TTGCTTCTGTGACCTACAAT+GGG - contig399end:65957-65976 None:intergenic 40.0%
TTTCTATTGTCACTCCACAG+CGG + contig399end:65231-65250 MsG0080048676.01.T01:intron 40.0%
TTTGCTTCTGTGACCTACAA+TGG - contig399end:65958-65977 None:intergenic 40.0%
! ACAGTGAAGGTACATACCTA+AGG + contig399end:64622-64641 MsG0080048676.01.T01:intron 40.0%
! AGTTTTTGCAGTCTGGATTG+AGG + contig399end:64001-64020 MsG0080048676.01.T01:intron 40.0%
! ATCAAGTGGTTTGGCTTAAG+TGG + contig399end:64252-64271 MsG0080048676.01.T01:intron 40.0%
! CTGTATGTCACAATTGGCTT+GGG + contig399end:66104-66123 MsG0080048676.01.T01:CDS 40.0%
! GCCAAAGATTGTTCTAGCTA+GGG - contig399end:64842-64861 None:intergenic 40.0%
! GTCAATCAGTTTTTGCAGTC+TGG + contig399end:63994-64013 MsG0080048676.01.T01:intron 40.0%
!! ATACATGCAGGAAAAGTACC+TGG - contig399end:65649-65668 None:intergenic 40.0%
!! TGAAGGGTGTGTTGAGATTT+TGG - contig399end:63577-63596 None:intergenic 40.0%
!!! AAGACTGAGCTTGTTTTGCA+AGG + contig399end:66344-66363 MsG0080048676.01.T01:three_prime_UTR 40.0%
!!! TGGCTATGTCCTTGTTTTTC+AGG + contig399end:65478-65497 MsG0080048676.01.T01:intron 40.0%
AATCGAACACCAACACCAAG+AGG - contig399end:63768-63787 None:intergenic 45.0%
ACACACCGTCCTGAAAAACA+AGG - contig399end:65490-65509 None:intergenic 45.0%
ACAGAACACACAAGCGTAGA+TGG - contig399end:65200-65219 None:intergenic 45.0%
ACCAAGAGGATAACCCTGTT+GGG - contig399end:63754-63773 None:intergenic 45.0%
ACTCCTCAGTGTACACTTCA+AGG + contig399end:64024-64043 MsG0080048676.01.T01:CDS 45.0%
CCTTCTCAAAATCCAGAGGA+TGG - contig399end:66146-66165 None:intergenic 45.0%
CCTTGACATGAAGTGAAGAG+GGG - contig399end:63872-63891 None:intergenic 45.0%
CTTGACATGAAGTGAAGAGG+GGG - contig399end:63871-63890 None:intergenic 45.0%
GATGATATGGGTAGACCGTT+TGG + contig399end:65978-65997 MsG0080048676.01.T01:CDS 45.0%
GCCCTAGCTAGAACAATCTT+TGG + contig399end:64838-64857 MsG0080048676.01.T01:intron 45.0%
GTTCCTTGAAGTGTACACTG+AGG - contig399end:64030-64049 None:intergenic 45.0%
GTTCTGGTAATCCAGAGTTG+AGG - contig399end:64362-64381 None:intergenic 45.0%
TTCGACGCCTACAATTTCAG+CGG - contig399end:64686-64705 None:intergenic 45.0%
TTCTCAGCAAACTCTCGAAG+GGG - contig399end:65551-65570 None:intergenic 45.0%
TTGATCAAAGCTGGATCCTG+CGG - contig399end:64063-64082 None:intergenic 45.0%
TTGGATTTGCCCATTTACGG+AGG - contig399end:63348-63367 None:intergenic 45.0%
! CTTCTCTAGGGAAAGGAATG+CGG - contig399end:66050-66069 None:intergenic 45.0%
! GGCCAAAGATTGTTCTAGCT+AGG - contig399end:64843-64862 None:intergenic 45.0%
! TCTGTGACTTCTCTAGGGAA+AGG - contig399end:66057-66076 None:intergenic 45.0%
!! CCATCCTCTGGATTTTGAGA+AGG + contig399end:66143-66162 MsG0080048676.01.T01:CDS 45.0%
!! TATGTCACAATTGGCTTGGG+AGG + contig399end:66107-66126 MsG0080048676.01.T01:CDS 45.0%
!!! TTGTTTTTCAGGACGGTGTG+TGG + contig399end:65489-65508 MsG0080048676.01.T01:intron 45.0%
!! ATATAATTTAATAAAAATGA+AGG - contig399end:65373-65392 None:intergenic 5.0%
!! TATAATTTAATAAAAATGAA+GGG - contig399end:65372-65391 None:intergenic 5.0%
ACCCAACAGGGTTATCCTCT+TGG + contig399end:63750-63769 MsG0080048676.01.T01:CDS 50.0%
ATCAACCATCCGGTTTCCAG+AGG - contig399end:64892-64911 None:intergenic 50.0%
ATCCGGTTTCCAGAGGAAAG+GGG - contig399end:64885-64904 None:intergenic 50.0%
CACAAAGAAACAAGCCGCTG+TGG - contig399end:65248-65267 None:intergenic 50.0%
CACCAAGAGGATAACCCTGT+TGG - contig399end:63755-63774 None:intergenic 50.0%
CATCCGGTTTCCAGAGGAAA+GGG - contig399end:64886-64905 None:intergenic 50.0%
CCCCTCTTCACTTCATGTCA+AGG + contig399end:63869-63888 MsG0080048676.01.T01:CDS 50.0%
GGATGACTTCCAGGAGGTTA+GGG + contig399end:65340-65359 MsG0080048676.01.T01:intron 50.0%
TCTCAGCAAACTCTCGAAGG+GGG - contig399end:65550-65569 None:intergenic 50.0%
TGGATGACTTCCAGGAGGTT+AGG + contig399end:65339-65358 MsG0080048676.01.T01:intron 50.0%
! ATGGGTAGACCGTTTGGGTT+TGG + contig399end:65984-66003 MsG0080048676.01.T01:CDS 50.0%
! CACAGCGGCTTGTTTCTTTG+TGG + contig399end:65246-65265 MsG0080048676.01.T01:intron 50.0%
! CAGGGTTATCCTCTTGGTGT+TGG + contig399end:63756-63775 MsG0080048676.01.T01:CDS 50.0%
! GCAGGATCCAGCTTTGATCA+AGG + contig399end:64062-64081 MsG0080048676.01.T01:CDS 50.0%
! GGCTCTGGTAGTCCAAAGTT+TGG - contig399end:64864-64883 None:intergenic 50.0%
! TTCTAGCTAGGGCTCGAACT+CGG - contig399end:64831-64850 None:intergenic 50.0%
!! TGTAGATAGGGTGCTGCACT+TGG + contig399end:65319-65338 MsG0080048676.01.T01:CDS 50.0%
CCATCCGGTTTCCAGAGGAA+AGG - contig399end:64887-64906 None:intergenic 55.0%
GCACTTGGATGACTTCCAGG+AGG + contig399end:65334-65353 MsG0080048676.01.T01:CDS 55.0%
GCTGCACTTGGATGACTTCC+AGG + contig399end:65331-65350 MsG0080048676.01.T01:CDS 55.0%
TCTAGCTAGGGCTCGAACTC+GGG - contig399end:64830-64849 None:intergenic 55.0%
TTTCCAGAGGAAAGGGGCTC+TGG - contig399end:64879-64898 None:intergenic 55.0%
! AGCCCCTTTCCTCTGGAAAC+CGG + contig399end:64880-64899 MsG0080048676.01.T01:intron 55.0%
! CCTTTCCTCTGGAAACCGGA+TGG + contig399end:64884-64903 MsG0080048676.01.T01:intron 55.0%
AGGAACGCTCACGAAACCGC+AGG + contig399end:64044-64063 MsG0080048676.01.T01:CDS 60.0%
CTACCAGAGCCCCTTTCCTC+TGG + contig399end:64873-64892 MsG0080048676.01.T01:intron 60.0%
GCAAACTCTCGAAGGGGGTC+AGG - contig399end:65545-65564 None:intergenic 60.0%
GGGTCAGGTCCCTAACCTCC+TGG - contig399end:65352-65371 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
contig399end gene 63335 66387 63335 ID=MsG0080048676.01;Name=MsG0080048676.01
contig399end mRNA 63335 66387 63335 ID=MsG0080048676.01.T01;Parent=MsG0080048676.01;Name=MsG0080048676.01.T01;_AED=0.39;_eAED=0.39;_QI=175|1|1|1|1|1|5|193|315
contig399end exon 65964 66387 65964 ID=MsG0080048676.01.T01:exon:626;Parent=MsG0080048676.01.T01
contig399end exon 65500 65649 65500 ID=MsG0080048676.01.T01:exon:625;Parent=MsG0080048676.01.T01
contig399end exon 65251 65355 65251 ID=MsG0080048676.01.T01:exon:624;Parent=MsG0080048676.01.T01
contig399end exon 64003 64083 64003 ID=MsG0080048676.01.T01:exon:623;Parent=MsG0080048676.01.T01
contig399end exon 63335 63890 63335 ID=MsG0080048676.01.T01:exon:622;Parent=MsG0080048676.01.T01
contig399end five_prime_UTR 63335 63509 63335 ID=MsG0080048676.01.T01:five_prime_utr;Parent=MsG0080048676.01.T01
contig399end CDS 63510 63890 63510 ID=MsG0080048676.01.T01:cds;Parent=MsG0080048676.01.T01
contig399end CDS 64003 64083 64003 ID=MsG0080048676.01.T01:cds;Parent=MsG0080048676.01.T01
contig399end CDS 65251 65355 65251 ID=MsG0080048676.01.T01:cds;Parent=MsG0080048676.01.T01
contig399end CDS 65500 65649 65500 ID=MsG0080048676.01.T01:cds;Parent=MsG0080048676.01.T01
contig399end CDS 65964 66194 65964 ID=MsG0080048676.01.T01:cds;Parent=MsG0080048676.01.T01
contig399end three_prime_UTR 66195 66387 66195 ID=MsG0080048676.01.T01:three_prime_utr;Parent=MsG0080048676.01.T01
Gene Sequence

>MsG0080048676.01.T01

ATGCTTCTTCAAACAAGAAACTTCACTGCACCATCATGTTTTACACCAACAACAAAACCAAACACCAAAATCTCAACACACCCTTCAAATTCAATCTCATTTCGTAACACTAAATGTTCTGTCACTGTTTCAACCCAATTAGAACAATCAACAATCGATAACAAAATCAAGAAACCGTTTCCATCTGAAGTTTCAAGAACAATTATGGATTTAGCTAGGGTTGGAACACTTTCAACGTTAACCCAACAGGGTTATCCTCTTGGTGTTGGTGTTCGATTCGTTATTGATTCTGAAGATGGAAACCCTTTTTTCTATTTCAATCATAATGCTATTCCCACCACTGATAATAACATTCACACCCCCTCTTCACTTCATGTCAAGTTTTTGCAGTCTGGATTGAGGACTCCTCAGTGTACACTTCAAGGAACGCTCACGAAACCGCAGGATCCAGCTTTGATCAAGCGGCTTGTTTCTTTGTGGAAGAAGAGGTTTGGAGAAGAAGTTAATCAAGATTTTATGTATATTATTGCTGTAGATAGGGTGCTGCACTTGGATGACTTCCAGGAGGACGGTGTGTGGTTCACCTCTTTAGACTACAAAAATGCTCAACCTGACCCCCTTCGAGAGTTTGCTGAGAATTTAGTCGCTGAAATTAACACCAATAATATGGAAGACATTACTCGCTTTTGTAATGTCTATGTTGATTTGGATTTCCAGGTCACAGAAGCAAAGATGATATGGGTAGACCGTTTGGGTTTGGATATGCGTTTGTTTTCTCCTAACAACGGTTTATTTGAGGTCCGCATTCCTTTCCCTAGAGAAGTCACAGATGAAAAAGGTGCCAAGTCAACATTTAACTGTATGTCACAATTGGCTTGGGAGGTAGAAAGAAACTTCCATCCTCTGGATTTTGAGAAGGTTACAGAATTGAAGCACATAAAGTCATGA
Protein sequence

>MsG0080048676.01.T01

MLLQTRNFTAPSCFTPTTKPNTKISTHPSNSISFRNTKCSVTVSTQLEQSTIDNKIKKPFPSEVSRTIMDLARVGTLSTLTQQGYPLGVGVRFVIDSEDGNPFFYFNHNAIPTTDNNIHTPSSLHVKFLQSGLRTPQCTLQGTLTKPQDPALIKRLVSLWKKRFGEEVNQDFMYIIAVDRVLHLDDFQEDGVWFTSLDYKNAQPDPLREFAENLVAEINTNNMEDITRFCNVYVDLDFQVTEAKMIWVDRLGLDMRLFSPNNGLFEVRIPFPREVTDEKGAKSTFNCMSQLAWEVERNFHPLDFEKVTELKHIKS*