Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007914.01.T01 | XP_003594838.3 | 97.452 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 6.73E-104 | 304 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007914.01.T01 | Q06931 | 84.713 | 157 | 24 | 0 | 1 | 157 | 1 | 157 | 2.32E-90 | 262 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007914.01.T01 | A0A396J759 | 97.452 | 157 | 4 | 0 | 1 | 157 | 1 | 157 | 3.21e-104 | 304 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001785.01 | MsG0280007914.01 | 0.833353 | 5.600507e-56 | 6.433676e-53 |
| MsG0180004154.01 | MsG0280007914.01 | 0.833248 | 5.948306e-56 | 6.811428e-53 |
| MsG0180004266.01 | MsG0280007914.01 | 0.840194 | 1.006783e-57 | 1.423365e-54 |
| MsG0180004777.01 | MsG0280007914.01 | 0.808076 | 3.698469e-50 | 2.094638e-47 |
| MsG0280007912.01 | MsG0280007914.01 | 0.821506 | 3.894208e-53 | 3.173974e-50 |
| MsG0280007914.01 | MsG0380015008.01 | 0.837223 | 5.899863e-57 | 7.612745e-54 |
| MsG0280007914.01 | MsG0380015009.01 | 0.841347 | 5.022373e-58 | 7.358285e-55 |
| MsG0280007914.01 | MsG0480022690.01 | -0.832253 | 1.050491e-55 | 1.167613e-52 |
| MsG0280007914.01 | MsG0480023819.01 | 0.811515 | 6.737369e-51 | 4.179074e-48 |
| MsG0280007914.01 | MsG0580025510.01 | 0.803148 | 4.002632e-49 | 1.996475e-46 |
| MsG0280007914.01 | MsG0580030135.01 | 0.802410 | 5.684849e-49 | 2.782822e-46 |
| MsG0280007914.01 | MsG0780036661.01 | 0.812857 | 3.432328e-51 | 2.206414e-48 |
| MsG0280007914.01 | MsG0780038525.01 | 0.800984 | 1.114927e-48 | 5.264230e-46 |
| MsG0280007914.01 | MsG0880047747.01 | 0.827962 | 1.170821e-54 | 1.147758e-51 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007914.01.T01 | MTR_2g035210 | 96.815 | 157 | 5 | 0 | 1 | 157 | 1 | 157 | 2.00e-107 | 303 |
| MsG0280007914.01.T01 | MTR_2g035220 | 93.631 | 157 | 10 | 0 | 1 | 157 | 1 | 157 | 2.74e-103 | 293 |
| MsG0280007914.01.T01 | MTR_2g035190 | 91.083 | 157 | 14 | 0 | 1 | 157 | 1 | 157 | 1.59e-100 | 285 |
| MsG0280007914.01.T01 | MTR_2g035320 | 88.535 | 157 | 18 | 0 | 1 | 157 | 1 | 157 | 7.00e-99 | 281 |
| MsG0280007914.01.T01 | MTR_6g033450 | 65.605 | 157 | 54 | 0 | 1 | 157 | 1 | 157 | 2.54e-68 | 204 |
| MsG0280007914.01.T01 | MTR_4g120970 | 65.605 | 157 | 54 | 0 | 1 | 157 | 1 | 157 | 2.54e-68 | 204 |
| MsG0280007914.01.T01 | MTR_2g035100 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 1.86e-64 | 194 |
| MsG0280007914.01.T01 | MTR_2g035105 | 61.146 | 157 | 61 | 0 | 1 | 157 | 1 | 157 | 1.47e-63 | 192 |
| MsG0280007914.01.T01 | MTR_2g035150 | 61.783 | 157 | 60 | 0 | 1 | 157 | 1 | 157 | 4.08e-63 | 191 |
| MsG0280007914.01.T01 | MTR_2g035130 | 59.236 | 157 | 64 | 0 | 1 | 157 | 1 | 157 | 3.74e-61 | 186 |
| MsG0280007914.01.T01 | MTR_2g035120 | 57.325 | 157 | 67 | 0 | 1 | 157 | 1 | 157 | 9.53e-58 | 177 |
| MsG0280007914.01.T01 | MTR_2g035320 | 87.500 | 96 | 12 | 0 | 62 | 157 | 27 | 122 | 2.55e-56 | 172 |
| MsG0280007914.01.T01 | MTR_2g035170 | 47.134 | 157 | 83 | 0 | 1 | 157 | 1 | 157 | 1.33e-46 | 149 |
| MsG0280007914.01.T01 | MTR_4g120760 | 35.032 | 157 | 101 | 1 | 1 | 156 | 1 | 157 | 1.03e-31 | 111 |
| MsG0280007914.01.T01 | MTR_4g120760 | 35.032 | 157 | 101 | 1 | 1 | 156 | 1 | 157 | 1.17e-31 | 111 |
| MsG0280007914.01.T01 | MTR_4g120950 | 40.373 | 161 | 89 | 5 | 1 | 157 | 1 | 158 | 7.05e-31 | 109 |
| MsG0280007914.01.T01 | MTR_2g435310 | 32.051 | 156 | 105 | 1 | 1 | 155 | 1 | 156 | 1.56e-30 | 108 |
| MsG0280007914.01.T01 | MTR_4g120940 | 61.644 | 73 | 28 | 0 | 85 | 157 | 47 | 119 | 2.85e-22 | 86.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCAACAATTTCAACATTC+TGG | 0.148922 | 2:-22850486 | None:intergenic |
| TGTTTAATTATCTAGTAATT+AGG | 0.228873 | 2:-22851480 | None:intergenic |
| GGCCATTGAGGGTTATGTTT+TGG | 0.290931 | 2:+22851452 | MsG0280007914.01.T01:CDS |
| CTTGATAAGCCCAGTTCCTT+TGG | 0.331420 | 2:-22851430 | None:intergenic |
| TGGGCAGCTGGAATCACCTT+TGG | 0.335275 | 2:-22850466 | None:intergenic |
| GGGCAGCTGGAATCACCTTT+GGG | 0.356589 | 2:-22850465 | None:intergenic |
| TTCAACAATTTCAACATTCT+GGG | 0.389410 | 2:-22850485 | None:intergenic |
| GTTGAAATTGTTGAAGGAAA+TGG | 0.389622 | 2:+22850493 | MsG0280007914.01.T01:CDS |
| AGAGCTTTGTAGAGTTTAGC+TGG | 0.394931 | 2:-22850418 | None:intergenic |
| AGCAGTGGATGAGGCAAACT+TGG | 0.402684 | 2:+22851233 | MsG0280007914.01.T01:CDS |
| TACAGCATAGTAGGAGGAAC+AGG | 0.409311 | 2:+22851264 | MsG0280007914.01.T01:CDS |
| GAAACTAAGACCAAAGGAAC+TGG | 0.409349 | 2:+22851420 | MsG0280007914.01.T01:CDS |
| TTTGAGACAAGTATTGTTGC+TGG | 0.423241 | 2:+22851315 | MsG0280007914.01.T01:CDS |
| AGATAGAACTGCATCACCTT+TGG | 0.441724 | 2:-22851382 | None:intergenic |
| ATTTCAACATTCTGGGCAGC+TGG | 0.462825 | 2:-22850478 | None:intergenic |
| GTTGGATGAAAGTTTAGAGA+AGG | 0.466544 | 2:+22851287 | MsG0280007914.01.T01:CDS |
| GGATACAACTACAGCATAGT+AGG | 0.484797 | 2:+22851255 | MsG0280007914.01.T01:CDS |
| GGGCTTATCAAGGCCATTGA+GGG | 0.488480 | 2:+22851441 | MsG0280007914.01.T01:CDS |
| CAGAATGTTGAAATTGTTGA+AGG | 0.492785 | 2:+22850487 | MsG0280007914.01.T01:CDS |
| CAAATTAGAAGCAGTGGATG+AGG | 0.520372 | 2:+22851224 | MsG0280007914.01.T01:CDS |
| TGCCAAAACATAACCCTCAA+TGG | 0.521409 | 2:-22851454 | None:intergenic |
| CAAAGGAACTGGGCTTATCA+AGG | 0.531479 | 2:+22851431 | MsG0280007914.01.T01:CDS |
| CTTTGGTCTTAGTTTCATCA+CGG | 0.561343 | 2:-22851413 | None:intergenic |
| AGTATTGTTGCTGGATCTGA+TGG | 0.562676 | 2:+22851324 | MsG0280007914.01.T01:CDS |
| TTTCACTGAAATCTTAACAA+TGG | 0.567421 | 2:-22851352 | None:intergenic |
| TAATTTGTGTAGCACATAGT+CGG | 0.573457 | 2:-22851208 | None:intergenic |
| TGGGCTTATCAAGGCCATTG+AGG | 0.577689 | 2:+22851440 | MsG0280007914.01.T01:CDS |
| GTTGAAGGAAATGGAGGACC+TGG | 0.587550 | 2:+22850502 | MsG0280007914.01.T01:CDS |
| GTAGAGTTTAGCTGGAGCCA+CGG | 0.602676 | 2:-22850410 | None:intergenic |
| GCTACACAAATTAGAAGCAG+TGG | 0.603203 | 2:+22851218 | MsG0280007914.01.T01:CDS |
| AAACTAAGACCAAAGGAACT+GGG | 0.604644 | 2:+22851421 | MsG0280007914.01.T01:CDS |
| TAATGAATTACCTTCAGACA+TGG | 0.606422 | 2:-22850542 | None:intergenic |
| CATAGTAGGAGGAACAGGGT+TGG | 0.609437 | 2:+22851269 | MsG0280007914.01.T01:CDS |
| TGCTGATGAAATCATCCCAA+AGG | 0.616025 | 2:+22850450 | MsG0280007914.01.T01:CDS |
| GAAATTGTTGAAGGAAATGG+AGG | 0.626795 | 2:+22850496 | MsG0280007914.01.T01:CDS |
| GCAGTGGATGAGGCAAACTT+GGG | 0.627060 | 2:+22851234 | MsG0280007914.01.T01:CDS |
| TACAACTACAGCATAGTAGG+AGG | 0.648903 | 2:+22851258 | MsG0280007914.01.T01:CDS |
| CGTGATGAAACTAAGACCAA+AGG | 0.649662 | 2:+22851414 | MsG0280007914.01.T01:CDS |
| TCAGTGAAATACCACACCAA+AGG | 0.661287 | 2:+22851366 | MsG0280007914.01.T01:CDS |
| ATTGTTGCTGGATCTGATGG+TGG | 0.674505 | 2:+22851327 | MsG0280007914.01.T01:CDS |
| GAACTGCATCACCTTTGGTG+TGG | 0.674778 | 2:-22851377 | None:intergenic |
| TGATGAACATGTCTCAACCG+TGG | 0.717160 | 2:+22850393 | MsG0280007914.01.T01:CDS |
| ACAGCATAGTAGGAGGAACA+GGG | 0.726335 | 2:+22851265 | MsG0280007914.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCTTATATATTTTTTTTCCA+TGG | - | Chr2:22850744-22850763 | None:intergenic | 15.0% |
| !! | AATTATGATGTTTGTATTGT+AGG | + | Chr2:22851177-22851196 | MsG0280007914.01.T01:intron | 20.0% |
| !!! | AATAAAGCCTTTTTTTCTTA+GGG | - | Chr2:22850584-22850603 | None:intergenic | 20.0% |
| !!! | TAATAAAGCCTTTTTTTCTT+AGG | - | Chr2:22850585-22850604 | None:intergenic | 20.0% |
| ! | ACTATAACCCTAAGAAAAAA+AGG | + | Chr2:22850574-22850593 | MsG0280007914.01.T01:intron | 25.0% |
| ! | AGAACCACTATTTGTATAAA+AGG | + | Chr2:22850784-22850803 | MsG0280007914.01.T01:intron | 25.0% |
| ! | GATATCATTAATCACTTGAT+CGG | - | Chr2:22851087-22851106 | None:intergenic | 25.0% |
| ! | TTCAACAATTTCAACATTCT+GGG | - | Chr2:22850488-22850507 | None:intergenic | 25.0% |
| ! | TTTCACTGAAATCTTAACAA+TGG | - | Chr2:22851355-22851374 | None:intergenic | 25.0% |
| !! | AGTTCCTTTTATACAAATAG+TGG | - | Chr2:22850791-22850810 | None:intergenic | 25.0% |
| !!! | AAACTTTACTTCTGTTTTGA+GGG | + | Chr2:22851140-22851159 | MsG0280007914.01.T01:intron | 25.0% |
| !!! | AAATAGATTTTGAGTGTTGA+AGG | + | Chr2:22850657-22850676 | MsG0280007914.01.T01:intron | 25.0% |
| !!! | AGACATGGATAACTTTTTTA+TGG | - | Chr2:22850530-22850549 | None:intergenic | 25.0% |
| AAAAAGTTATCCATGTCTGA+AGG | + | Chr2:22850532-22850551 | MsG0280007914.01.T01:CDS | 30.0% | |
| AGACTCAGTTTATGTATCAT+TGG | - | Chr2:22850686-22850705 | None:intergenic | 30.0% | |
| CAAGAAACAACTAATTTCCA+TGG | + | Chr2:22850724-22850743 | MsG0280007914.01.T01:intron | 30.0% | |
| CAGAATGTTGAAATTGTTGA+AGG | + | Chr2:22850487-22850506 | MsG0280007914.01.T01:CDS | 30.0% | |
| CTTCAACAATTTCAACATTC+TGG | - | Chr2:22850489-22850508 | None:intergenic | 30.0% | |
| GTTGAAATTGTTGAAGGAAA+TGG | + | Chr2:22850493-22850512 | MsG0280007914.01.T01:CDS | 30.0% | |
| TAATGAATTACCTTCAGACA+TGG | - | Chr2:22850545-22850564 | None:intergenic | 30.0% | |
| TATGATGTTTGTATTGTAGG+TGG | + | Chr2:22851180-22851199 | MsG0280007914.01.T01:intron | 30.0% | |
| ! | CAAACTTTACTTCTGTTTTG+AGG | + | Chr2:22851139-22851158 | MsG0280007914.01.T01:intron | 30.0% |
| ! | TAATTTGTGTAGCACATAGT+CGG | - | Chr2:22851211-22851230 | None:intergenic | 30.0% |
| AAACTAAGACCAAAGGAACT+GGG | + | Chr2:22851421-22851440 | MsG0280007914.01.T01:CDS | 35.0% | |
| GAAATTGTTGAAGGAAATGG+AGG | + | Chr2:22850496-22850515 | MsG0280007914.01.T01:CDS | 35.0% | |
| GTTGGATGAAAGTTTAGAGA+AGG | + | Chr2:22851287-22851306 | MsG0280007914.01.T01:CDS | 35.0% | |
| TTGTAGACCACACAATTTGT+TGG | - | Chr2:22851026-22851045 | None:intergenic | 35.0% | |
| ! | TGTAGACCACACAATTTGTT+GGG | - | Chr2:22851025-22851044 | None:intergenic | 35.0% |
| ! | TTTGAGACAAGTATTGTTGC+TGG | + | Chr2:22851315-22851334 | MsG0280007914.01.T01:CDS | 35.0% |
| !! | CTTTGGTCTTAGTTTCATCA+CGG | - | Chr2:22851416-22851435 | None:intergenic | 35.0% |
| !!! | GATAACTTTTTTATGGTGCC+AGG | - | Chr2:22850523-22850542 | None:intergenic | 35.0% |
| AATCACCCCAACAAATTGTG+TGG | + | Chr2:22851016-22851035 | MsG0280007914.01.T01:intron | 40.0% | |
| AGATAGAACTGCATCACCTT+TGG | - | Chr2:22851385-22851404 | None:intergenic | 40.0% | |
| AGTATTGTTGCTGGATCTGA+TGG | + | Chr2:22851324-22851343 | MsG0280007914.01.T01:CDS | 40.0% | |
| CAAATTAGAAGCAGTGGATG+AGG | + | Chr2:22851224-22851243 | MsG0280007914.01.T01:CDS | 40.0% | |
| CGTGATGAAACTAAGACCAA+AGG | + | Chr2:22851414-22851433 | MsG0280007914.01.T01:CDS | 40.0% | |
| GAAACTAAGACCAAAGGAAC+TGG | + | Chr2:22851420-22851439 | MsG0280007914.01.T01:CDS | 40.0% | |
| GCTACACAAATTAGAAGCAG+TGG | + | Chr2:22851218-22851237 | MsG0280007914.01.T01:CDS | 40.0% | |
| GGATACAACTACAGCATAGT+AGG | + | Chr2:22851255-22851274 | MsG0280007914.01.T01:CDS | 40.0% | |
| TACAACTACAGCATAGTAGG+AGG | + | Chr2:22851258-22851277 | MsG0280007914.01.T01:CDS | 40.0% | |
| TCAGTGAAATACCACACCAA+AGG | + | Chr2:22851366-22851385 | MsG0280007914.01.T01:CDS | 40.0% | |
| TGCCAAAACATAACCCTCAA+TGG | - | Chr2:22851457-22851476 | None:intergenic | 40.0% | |
| TGCTGATGAAATCATCCCAA+AGG | + | Chr2:22850450-22850469 | MsG0280007914.01.T01:CDS | 40.0% | |
| ! | AGAGCTTTGTAGAGTTTAGC+TGG | - | Chr2:22850421-22850440 | None:intergenic | 40.0% |
| !! | GTAGACCACACAATTTGTTG+GGG | - | Chr2:22851024-22851043 | None:intergenic | 40.0% |
| ACAGCATAGTAGGAGGAACA+GGG | + | Chr2:22851265-22851284 | MsG0280007914.01.T01:CDS | 45.0% | |
| ATTGTTGCTGGATCTGATGG+TGG | + | Chr2:22851327-22851346 | MsG0280007914.01.T01:CDS | 45.0% | |
| ATTTCAACATTCTGGGCAGC+TGG | - | Chr2:22850481-22850500 | None:intergenic | 45.0% | |
| CAAAGGAACTGGGCTTATCA+AGG | + | Chr2:22851431-22851450 | MsG0280007914.01.T01:CDS | 45.0% | |
| CTTGATAAGCCCAGTTCCTT+TGG | - | Chr2:22851433-22851452 | None:intergenic | 45.0% | |
| TACAGCATAGTAGGAGGAAC+AGG | + | Chr2:22851264-22851283 | MsG0280007914.01.T01:CDS | 45.0% | |
| TGATGAACATGTCTCAACCG+TGG | + | Chr2:22850393-22850412 | MsG0280007914.01.T01:CDS | 45.0% | |
| ! | GGCCATTGAGGGTTATGTTT+TGG | + | Chr2:22851452-22851471 | MsG0280007914.01.T01:CDS | 45.0% |
| AGCAGTGGATGAGGCAAACT+TGG | + | Chr2:22851233-22851252 | MsG0280007914.01.T01:CDS | 50.0% | |
| CATAGTAGGAGGAACAGGGT+TGG | + | Chr2:22851269-22851288 | MsG0280007914.01.T01:CDS | 50.0% | |
| GAACTGCATCACCTTTGGTG+TGG | - | Chr2:22851380-22851399 | None:intergenic | 50.0% | |
| GCAGTGGATGAGGCAAACTT+GGG | + | Chr2:22851234-22851253 | MsG0280007914.01.T01:CDS | 50.0% | |
| GGGCTTATCAAGGCCATTGA+GGG | + | Chr2:22851441-22851460 | MsG0280007914.01.T01:CDS | 50.0% | |
| GTAGAGTTTAGCTGGAGCCA+CGG | - | Chr2:22850413-22850432 | None:intergenic | 50.0% | |
| GTTGAAGGAAATGGAGGACC+TGG | + | Chr2:22850502-22850521 | MsG0280007914.01.T01:CDS | 50.0% | |
| TGGGCTTATCAAGGCCATTG+AGG | + | Chr2:22851440-22851459 | MsG0280007914.01.T01:CDS | 50.0% | |
| GGGCAGCTGGAATCACCTTT+GGG | - | Chr2:22850468-22850487 | None:intergenic | 55.0% | |
| TGGGCAGCTGGAATCACCTT+TGG | - | Chr2:22850469-22850488 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 22850373 | 22851491 | 22850373 | ID=MsG0280007914.01;Name=MsG0280007914.01 |
| Chr2 | mRNA | 22850373 | 22851491 | 22850373 | ID=MsG0280007914.01.T01;Parent=MsG0280007914.01;Name=MsG0280007914.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|157 |
| Chr2 | exon | 22850373 | 22850553 | 22850373 | ID=MsG0280007914.01.T01:exon:13882;Parent=MsG0280007914.01.T01 |
| Chr2 | exon | 22851199 | 22851491 | 22851199 | ID=MsG0280007914.01.T01:exon:13883;Parent=MsG0280007914.01.T01 |
| Chr2 | CDS | 22850373 | 22850553 | 22850373 | ID=MsG0280007914.01.T01:cds;Parent=MsG0280007914.01.T01 |
| Chr2 | CDS | 22851199 | 22851491 | 22851199 | ID=MsG0280007914.01.T01:cds;Parent=MsG0280007914.01.T01 |
| Gene Sequence |
| Protein sequence |
