Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025549.01.T01 | XP_003612898.1 | 87.395 | 119 | 5 | 1 | 33 | 151 | 12 | 120 | 3.61E-66 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025549.01.T01 | G7KF21 | 87.395 | 119 | 5 | 1 | 33 | 151 | 12 | 120 | 1.72e-66 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280010586.01 | MsG0580025549.01 | 0.810082 | 1.375886e-50 | 8.216316e-48 |
| MsG0380013342.01 | MsG0580025549.01 | 0.808005 | 3.829262e-50 | 2.164678e-47 |
| MsG0480018995.01 | MsG0580025549.01 | 0.812405 | 4.310969e-51 | 2.738725e-48 |
| MsG0480020322.01 | MsG0580025549.01 | 0.853242 | 2.720683e-61 | 5.840249e-58 |
| MsG0480020323.01 | MsG0580025549.01 | 0.863571 | 2.264632e-64 | 6.896493e-61 |
| MsG0580025523.01 | MsG0580025549.01 | 0.874797 | 5.097913e-68 | 2.326005e-64 |
| MsG0580025548.01 | MsG0580025549.01 | 0.862068 | 6.582793e-64 | 1.902803e-60 |
| MsG0580025549.01 | MsG0880041999.01 | 0.841044 | 6.032289e-58 | 8.754859e-55 |
| MsG0580025549.01 | MsG0880042031.01 | 0.813140 | 2.975199e-51 | 1.927129e-48 |
| MsG0580025549.01 | MsG0880047267.01 | 0.820024 | 8.535228e-53 | 6.676927e-50 |
| MsG0380016285.01 | MsG0580025549.01 | 0.808488 | 3.021559e-50 | 1.729937e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0580025549.01.T01 | MTR_5g430430 | 87.395 | 119 | 5 | 1 | 33 | 151 | 12 | 120 | 4.37e-70 | 207 |
| MsG0580025549.01.T01 | MTR_5g030290 | 87.395 | 119 | 5 | 1 | 33 | 151 | 12 | 120 | 4.37e-70 | 207 |
| MsG0580025549.01.T01 | MTR_5g030310 | 77.311 | 119 | 17 | 1 | 33 | 151 | 12 | 120 | 1.15e-60 | 183 |
| MsG0580025549.01.T01 | MTR_5g430440 | 76.471 | 119 | 18 | 1 | 33 | 151 | 12 | 120 | 2.12e-59 | 180 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGCTCTTGAGTGCAGCTT+TGG | 0.250449 | 5:+20328840 | None:intergenic |
| CTCATATCATTGTGAGAATA+TGG | 0.263715 | 5:-20328867 | MsG0580025549.01.T01:CDS |
| TCACTGCTACTCTCAATTTC+AGG | 0.267344 | 5:+20327957 | None:intergenic |
| TTGCTCTTGAGTGCAGCTTT+GGG | 0.361214 | 5:+20328841 | None:intergenic |
| ATTGAGAGTAGCAGTGATAA+TGG | 0.416426 | 5:-20327951 | MsG0580025549.01.T01:CDS |
| CCCTTGCCTGAGTCTGCATC+AGG | 0.461443 | 5:+20327867 | None:intergenic |
| GGCAATGTGTTGTGCAAGAC+TGG | 0.472681 | 5:-20328634 | MsG0580025549.01.T01:CDS |
| TGTGTTATAACTAGGACTAC+TGG | 0.487878 | 5:-20328598 | MsG0580025549.01.T01:CDS |
| GCTCTACCTCATTCACTAGT+TGG | 0.491345 | 5:-20328935 | MsG0580025549.01.T01:five_prime_UTR |
| TAAACCAATAAGCTTTGTGC+AGG | 0.493645 | 5:-20328696 | MsG0580025549.01.T01:intron |
| ATCACTGCATGCATATAAGT+TGG | 0.527231 | 5:+20327995 | None:intergenic |
| AGGACTACTGGACCTGATGC+TGG | 0.536873 | 5:-20328586 | MsG0580025549.01.T01:CDS |
| AACTTATATGCATGCAGTGA+TGG | 0.542139 | 5:-20327993 | MsG0580025549.01.T01:CDS |
| CAAAGCTGCACTCAAGAGCA+AGG | 0.561178 | 5:-20328839 | MsG0580025549.01.T01:CDS |
| AATGTACCAACTAGTGAATG+AGG | 0.566165 | 5:+20328929 | None:intergenic |
| TCCTGATGCAGACTCAGGCA+AGG | 0.574927 | 5:-20327868 | MsG0580025549.01.T01:CDS |
| GAAGGTCCTGATGCAGACTC+AGG | 0.576340 | 5:-20327873 | MsG0580025549.01.T01:CDS |
| GTTGGACATGTGTTATAACT+AGG | 0.581816 | 5:-20328606 | MsG0580025549.01.T01:CDS |
| TCTGCAAGTGTGGTGAAGGA+TGG | 0.591787 | 5:-20327917 | MsG0580025549.01.T01:CDS |
| ACATTCTGCAAGTGTGGTGA+AGG | 0.593456 | 5:-20327921 | MsG0580025549.01.T01:CDS |
| GTGTGTGACATACAGTGTGC+AGG | 0.603372 | 5:+20328531 | None:intergenic |
| GTGTTGTGCAAGACTGGTAA+AGG | 0.626364 | 5:-20328628 | MsG0580025549.01.T01:CDS |
| CCTGATGCAGACTCAGGCAA+GGG | 0.633802 | 5:-20327867 | MsG0580025549.01.T01:CDS |
| TTATATGCATGCAGTGATGG+TGG | 0.638654 | 5:-20327990 | MsG0580025549.01.T01:CDS |
| AAAGCTGCACTCAAGAGCAA+GGG | 0.647133 | 5:-20328838 | MsG0580025549.01.T01:intron |
| ACAACACATTGCCCAAACCC+AGG | 0.649057 | 5:+20328644 | None:intergenic |
| TGTGCAAGACTGGTAAAGGT+TGG | 0.662647 | 5:-20328624 | MsG0580025549.01.T01:CDS |
| AATGAAACATTCTGCAAGTG+TGG | 0.667983 | 5:-20327927 | MsG0580025549.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACTATATTGATGAAATA+AGG | + | Chr5:20327988-20328007 | None:intergenic | 15.0% |
| !!! | TTCATTTTTTAGATTGAATT+GGG | - | Chr5:20328436-20328455 | MsG0580025549.01.T01:intron | 15.0% |
| !!! | TTTCATTTTTTAGATTGAAT+TGG | - | Chr5:20328435-20328454 | MsG0580025549.01.T01:intron | 15.0% |
| !! | ATTAACTCTTGAGATAAAAT+CGG | - | Chr5:20328665-20328684 | MsG0580025549.01.T01:CDS | 20.0% |
| !! | GATGCTAGTAAAATTAAAAT+AGG | + | Chr5:20328279-20328298 | None:intergenic | 20.0% |
| !! | GATTTATTTATGCATGAAAA+TGG | + | Chr5:20328052-20328071 | None:intergenic | 20.0% |
| !! | GTAAAATTAAAATAGGCAAA+AGG | + | Chr5:20328272-20328291 | None:intergenic | 20.0% |
| !!! | AATCAATTTTTTCTATCTCA+TGG | - | Chr5:20328717-20328736 | MsG0580025549.01.T01:intron | 20.0% |
| !!! | TTTTTCAAAATCGAATGAAT+TGG | - | Chr5:20328346-20328365 | MsG0580025549.01.T01:intron | 20.0% |
| ! | AAATGAACTCTGAATCAAAT+CGG | + | Chr5:20328576-20328595 | None:intergenic | 25.0% |
| ! | AATGAAACTGCAAAGTAATA+AGG | + | Chr5:20328422-20328441 | None:intergenic | 25.0% |
| AATAAGGATCAAATCGATAG+AGG | + | Chr5:20328406-20328425 | None:intergenic | 30.0% | |
| ACTTTGAATCAGTTTCATGT+CGG | - | Chr5:20328641-20328660 | MsG0580025549.01.T01:CDS | 30.0% | |
| ATAGTTCAAGACAAAAACTC+AGG | - | Chr5:20328106-20328125 | MsG0580025549.01.T01:intron | 30.0% | |
| ATCCGATTCAACTATTGTTA+CGG | - | Chr5:20328539-20328558 | MsG0580025549.01.T01:intron | 30.0% | |
| ATCTAGCAGTGCAATATATA+TGG | + | Chr5:20328753-20328772 | None:intergenic | 30.0% | |
| CTCATATCATTGTGAGAATA+TGG | - | Chr5:20327906-20327925 | MsG0580025549.01.T01:CDS | 30.0% | |
| TACCGTAACAATAGTTGAAT+CGG | + | Chr5:20328544-20328563 | None:intergenic | 30.0% | |
| TCAAAATCGAATGAATTGGT+TGG | - | Chr5:20328350-20328369 | MsG0580025549.01.T01:intron | 30.0% | |
| TTGATTGTATAAGCCTTGAA+TGG | + | Chr5:20328703-20328722 | None:intergenic | 30.0% | |
| ! | AAATGGAACATGAAGAGTAT+TGG | + | Chr5:20328035-20328054 | None:intergenic | 30.0% |
| ! | GGCAAAGTTTTTGTTAAATG+CGG | - | Chr5:20328208-20328227 | MsG0580025549.01.T01:intron | 30.0% |
| AACTTATATGCATGCAGTGA+TGG | - | Chr5:20328780-20328799 | MsG0580025549.01.T01:intron | 35.0% | |
| AATGAAACATTCTGCAAGTG+TGG | - | Chr5:20328846-20328865 | MsG0580025549.01.T01:CDS | 35.0% | |
| AATGTACCAACTAGTGAATG+AGG | + | Chr5:20327847-20327866 | None:intergenic | 35.0% | |
| ATCACTGCATGCATATAAGT+TGG | + | Chr5:20328781-20328800 | None:intergenic | 35.0% | |
| ATTGAGAGTAGCAGTGATAA+TGG | - | Chr5:20328822-20328841 | MsG0580025549.01.T01:intron | 35.0% | |
| GTTGGACATGTGTTATAACT+AGG | - | Chr5:20328167-20328186 | MsG0580025549.01.T01:intron | 35.0% | |
| TGTGTTATAACTAGGACTAC+TGG | - | Chr5:20328175-20328194 | MsG0580025549.01.T01:intron | 35.0% | |
| ! | AATGAATTGGTTGGTTCAAC+TGG | - | Chr5:20328359-20328378 | MsG0580025549.01.T01:intron | 35.0% |
| ! | TAAACCAATAAGCTTTGTGC+AGG | - | Chr5:20328077-20328096 | MsG0580025549.01.T01:intron | 35.0% |
| !! | GATGGAGTTGTTCCATTTTT+AGG | - | Chr5:20328874-20328893 | MsG0580025549.01.T01:CDS | 35.0% |
| !!! | TGTTCCATTTTTAGGACTGA+AGG | - | Chr5:20328882-20328901 | MsG0580025549.01.T01:CDS | 35.0% |
| ACAAAAACTTTGCCAGCATC+AGG | + | Chr5:20328202-20328221 | None:intergenic | 40.0% | |
| AGGACCTTCAGTCCTAAAAA+TGG | + | Chr5:20328889-20328908 | None:intergenic | 40.0% | |
| CTTTCCTGCACAAAGCTTAT+TGG | + | Chr5:20328084-20328103 | None:intergenic | 40.0% | |
| TCACTGCTACTCTCAATTTC+AGG | + | Chr5:20328819-20328838 | None:intergenic | 40.0% | |
| TTATATGCATGCAGTGATGG+TGG | - | Chr5:20328783-20328802 | MsG0580025549.01.T01:intron | 40.0% | |
| ! | GTTCGATTTGAGACCATTCA+AGG | - | Chr5:20328687-20328706 | MsG0580025549.01.T01:CDS | 40.0% |
| AAAAACTCAGGCCTGGGTTT+GGG | - | Chr5:20328118-20328137 | MsG0580025549.01.T01:intron | 45.0% | |
| AAAGCTGCACTCAAGAGCAA+GGG | - | Chr5:20327935-20327954 | MsG0580025549.01.T01:CDS | 45.0% | |
| ACATTCTGCAAGTGTGGTGA+AGG | - | Chr5:20328852-20328871 | MsG0580025549.01.T01:CDS | 45.0% | |
| CAAGACAAAAACTCAGGCCT+GGG | - | Chr5:20328112-20328131 | MsG0580025549.01.T01:intron | 45.0% | |
| GCTCTACCTCATTCACTAGT+TGG | - | Chr5:20327838-20327857 | MsG0580025549.01.T01:CDS | 45.0% | |
| GTGTTGTGCAAGACTGGTAA+AGG | - | Chr5:20328145-20328164 | MsG0580025549.01.T01:intron | 45.0% | |
| TCAAGACAAAAACTCAGGCC+TGG | - | Chr5:20328111-20328130 | MsG0580025549.01.T01:intron | 45.0% | |
| TGTGCAAGACTGGTAAAGGT+TGG | - | Chr5:20328149-20328168 | MsG0580025549.01.T01:intron | 45.0% | |
| TTGCTCTTGAGTGCAGCTTT+GGG | + | Chr5:20327935-20327954 | None:intergenic | 45.0% | |
| !!! | ATTTTTAATCTTAAATTTAT+CGG | + | Chr5:20328608-20328627 | None:intergenic | 5.0% |
| ACAACACATTGCCCAAACCC+AGG | + | Chr5:20328132-20328151 | None:intergenic | 50.0% | |
| CAAAAACTCAGGCCTGGGTT+TGG | - | Chr5:20328117-20328136 | MsG0580025549.01.T01:intron | 50.0% | |
| CAAAGCTGCACTCAAGAGCA+AGG | - | Chr5:20327934-20327953 | MsG0580025549.01.T01:CDS | 50.0% | |
| CTTGCTCTTGAGTGCAGCTT+TGG | + | Chr5:20327936-20327955 | None:intergenic | 50.0% | |
| GGCAATGTGTTGTGCAAGAC+TGG | - | Chr5:20328139-20328158 | MsG0580025549.01.T01:intron | 50.0% | |
| GTGTGTGACATACAGTGTGC+AGG | + | Chr5:20328245-20328264 | None:intergenic | 50.0% | |
| TCTGCAAGTGTGGTGAAGGA+TGG | - | Chr5:20328856-20328875 | MsG0580025549.01.T01:CDS | 50.0% | |
| AGGACTACTGGACCTGATGC+TGG | - | Chr5:20328187-20328206 | MsG0580025549.01.T01:intron | 55.0% | |
| CCTGATGCAGACTCAGGCAA+GGG | - | Chr5:20328906-20328925 | MsG0580025549.01.T01:exon | 55.0% | |
| GAAGGTCCTGATGCAGACTC+AGG | - | Chr5:20328900-20328919 | MsG0580025549.01.T01:CDS | 55.0% | |
| TCCTGATGCAGACTCAGGCA+AGG | - | Chr5:20328905-20328924 | MsG0580025549.01.T01:exon | 55.0% | |
| CCCTTGCCTGAGTCTGCATC+AGG | + | Chr5:20328909-20328928 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr5 | gene | 20327821 | 20328974 | 20327821 | ID=MsG0580025549.01;Name=MsG0580025549.01 |
| Chr5 | mRNA | 20327821 | 20328974 | 20327821 | ID=MsG0580025549.01.T01;Parent=MsG0580025549.01;Name=MsG0580025549.01.T01;_AED=0.49;_eAED=0.53;_QI=51|0|0.33|1|0|0.33|3|0|151 |
| Chr5 | exon | 20327821 | 20328024 | 20327821 | ID=MsG0580025549.01.T01:exon:8899;Parent=MsG0580025549.01.T01 |
| Chr5 | exon | 20328540 | 20328706 | 20328540 | ID=MsG0580025549.01.T01:exon:8898;Parent=MsG0580025549.01.T01 |
| Chr5 | exon | 20328839 | 20328974 | 20328839 | ID=MsG0580025549.01.T01:exon:8897;Parent=MsG0580025549.01.T01 |
| Chr5 | five_prime_UTR | 20328924 | 20328974 | 20328924 | ID=MsG0580025549.01.T01:five_prime_utr;Parent=MsG0580025549.01.T01 |
| Chr5 | CDS | 20328839 | 20328923 | 20328839 | ID=MsG0580025549.01.T01:cds;Parent=MsG0580025549.01.T01 |
| Chr5 | CDS | 20328540 | 20328706 | 20328540 | ID=MsG0580025549.01.T01:cds;Parent=MsG0580025549.01.T01 |
| Chr5 | CDS | 20327821 | 20328024 | 20327821 | ID=MsG0580025549.01.T01:cds;Parent=MsG0580025549.01.T01 |
| Gene Sequence |
| Protein sequence |
