AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580025548.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580025548.01.T01 MTR_5g430430 93.578 109 7 0 31 139 12 120 4.74e-72 211
MsG0580025548.01.T01 MTR_5g030290 93.578 109 7 0 31 139 12 120 4.74e-72 211
MsG0580025548.01.T01 MTR_5g030310 83.486 109 18 0 31 139 12 120 7.20e-63 188
MsG0580025548.01.T01 MTR_5g430440 82.569 109 19 0 31 139 12 120 1.02e-61 185
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 38 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATTAAATTGTACTAAATT+TGG 0.161551 5:-20321224 MsG0580025548.01.T01:three_prime_UTR
TCACTTCTCTAGGGACAATT+TGG 0.245955 5:+20321285 None:intergenic
CTTGCTCTTGAGTGCAGCTT+TGG 0.250449 5:+20322386 None:intergenic
TCACTGCTACTCTCAATTTC+AGG 0.257468 5:+20321447 None:intergenic
CTCATATCATTGTGAGAATA+TGG 0.263715 5:-20322413 MsG0580025548.01.T01:CDS
TTGCTCTTGAGTGCAGCTTT+GGG 0.361214 5:+20322387 None:intergenic
TGTGTGATCGGCTATGAATT+TGG 0.386297 5:-20321185 MsG0580025548.01.T01:three_prime_UTR
AGGGTATCTAATGGCATGTT+TGG 0.402958 5:-20323003 None:intergenic
TTCAAGTTAATAAAATATCA+AGG 0.452541 5:-20321248 MsG0580025548.01.T01:three_prime_UTR
TTTGTAATTAGTAATGTGTT+TGG 0.455176 5:-20321151 MsG0580025548.01.T01:three_prime_UTR
GGCAGTGTGTTGTGCAAGAC+TGG 0.455435 5:-20322138 MsG0580025548.01.T01:CDS
CCCTTGCCTGAGTCTGCATC+AGG 0.461443 5:+20321357 None:intergenic
GTAATGCAATAGTATGTGTT+TGG 0.464999 5:-20321113 MsG0580025548.01.T01:three_prime_UTR
CCTTAGTGAATCACTTCTCT+AGG 0.478513 5:+20321275 None:intergenic
TACTAAATTTGGTTTATCAT+CGG 0.484979 5:-20321213 MsG0580025548.01.T01:three_prime_UTR
TGTGTTATAACTAGGACTAC+TGG 0.487878 5:-20322102 MsG0580025548.01.T01:CDS
TAAACCAATAAGCTTTGTGC+AGG 0.493645 5:-20322200 MsG0580025548.01.T01:intron
CCTAGAGAAGTGATTCACTA+AGG 0.524929 5:-20321275 MsG0580025548.01.T01:three_prime_UTR
ATAAGCTTTGTGCAGGAAAG+TGG 0.528788 5:-20322193 MsG0580025548.01.T01:intron
AGGACTACTGGACCTGATGC+TGG 0.536873 5:-20322090 MsG0580025548.01.T01:CDS
CAAAGCTGCACTCAAGAGCA+AGG 0.561178 5:-20322385 MsG0580025548.01.T01:CDS
TCATCGGTATTATGTGTGAT+CGG 0.569387 5:-20321197 MsG0580025548.01.T01:three_prime_UTR
TCCTGATGCAGACTCAGGCA+AGG 0.574927 5:-20321358 MsG0580025548.01.T01:CDS
GAAGGTCCTGATGCAGACTC+AGG 0.576340 5:-20321363 MsG0580025548.01.T01:CDS
CTAGAGAAGTGATTCACTAA+GGG 0.578373 5:-20321274 MsG0580025548.01.T01:three_prime_UTR
GTTGGACATGTGTTATAACT+AGG 0.581816 5:-20322110 MsG0580025548.01.T01:CDS
TCTGCAAGTGTGGTGAAGGA+TGG 0.591787 5:-20321407 MsG0580025548.01.T01:CDS
ACATTCTGCAAGTGTGGTGA+AGG 0.593456 5:-20321411 MsG0580025548.01.T01:CDS
CTTAGTGAATCACTTCTCTA+GGG 0.599847 5:+20321276 None:intergenic
ACAACACACTGCCCAAACCC+AGG 0.603905 5:+20322148 None:intergenic
ATTGAGAGTAGCAGTGACAA+TGG 0.620823 5:-20321441 MsG0580025548.01.T01:CDS
TTATATGCATGCAGTGATGG+TGG 0.622136 5:-20321480 MsG0580025548.01.T01:intron
GTGTGTGACATACAGTGTGC+AGG 0.624270 5:+20322035 None:intergenic
GTGTTGTGCAAGACTGGTAA+AGG 0.626364 5:-20322132 MsG0580025548.01.T01:CDS
CCTGATGCAGACTCAGGCAA+GGG 0.633802 5:-20321357 MsG0580025548.01.T01:CDS
AAAGCTGCACTCAAGAGCAA+GGG 0.647133 5:-20322384 MsG0580025548.01.T01:intron
TGTGCAAGACTGGTAAAGGT+TGG 0.662647 5:-20322128 MsG0580025548.01.T01:CDS
AGTGAAACATTCTGCAAGTG+TGG 0.671634 5:-20321417 MsG0580025548.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATTAAATTGTACTAAATT+TGG - Chr5:20322808-20322827 MsG0580025548.01.T01:intron 10.0%
!! AAAACTATATAGATGAAATA+AGG + Chr5:20321736-20321755 None:intergenic 15.0%
!! GATTTATTTATGTATGAAAA+TGG + Chr5:20321807-20321826 None:intergenic 15.0%
!! TATTCTAATAGCAAAAATTA+TGG + Chr5:20321355-20321374 None:intergenic 15.0%
!!! TTCAAGTTAATAAAATATCA+AGG - Chr5:20322784-20322803 MsG0580025548.01.T01:intron 15.0%
!!! TTCATTTTTTAGATTGAATT+GGG - Chr5:20322191-20322210 MsG0580025548.01.T01:CDS 15.0%
!!! TTTCATTTTTTAGATTGAAT+TGG - Chr5:20322190-20322209 MsG0580025548.01.T01:CDS 15.0%
!!! TTTTATACACTACTAATAAA+TGG + Chr5:20321421-20321440 None:intergenic 15.0%
!!! TTTTTAAAAATCGAATGAAT+TGG - Chr5:20322101-20322120 MsG0580025548.01.T01:CDS 15.0%
!!! TTTTTTTTTCTTCTCTTTAT+TGG - Chr5:20321208-20321227 MsG0580025548.01.T01:three_prime_UTR 15.0%
!! AATTGAACTCTAAATCAAAT+CGG + Chr5:20322323-20322342 None:intergenic 20.0%
!! ATGATATCATTGTTAATGTT+TGG - Chr5:20321291-20321310 MsG0580025548.01.T01:three_prime_UTR 20.0%
!! ATTAACTCTTGAGATAAAAT+CGG - Chr5:20322416-20322435 MsG0580025548.01.T01:CDS 20.0%
!! GATGCTAGTAAAATTAAAAT+AGG + Chr5:20322034-20322053 None:intergenic 20.0%
!! GTAAAATTAAAATAGGCAAA+AGG + Chr5:20322027-20322046 None:intergenic 20.0%
!! TTTGTAATTAGTAATGTGTT+TGG - Chr5:20322881-20322900 MsG0580025548.01.T01:intron 20.0%
!!! AATCAATTTTTTCTATCTCA+TGG - Chr5:20322484-20322503 MsG0580025548.01.T01:intron 20.0%
!!! TACTAAATTTGGTTTATCAT+CGG - Chr5:20322819-20322838 MsG0580025548.01.T01:intron 20.0%
! AACTGAATATTAGAGTAGTA+TGG + Chr5:20321702-20321721 None:intergenic 25.0%
! AATGAAACTGCAAAGTAATA+AGG + Chr5:20322177-20322196 None:intergenic 25.0%
! ACTGAATATTAGAGTAGTAT+GGG + Chr5:20321701-20321720 None:intergenic 25.0%
! TAAAAATCGAATGAATTGGT+TGG - Chr5:20322105-20322124 MsG0580025548.01.T01:CDS 25.0%
! TAATAAAATTGCGATGAGAA+CGG + Chr5:20321090-20321109 None:intergenic 25.0%
!! ATCTAGAAGTGCAATATATA+TGG + Chr5:20322520-20322539 None:intergenic 25.0%
AAAATGGAACAAGAGTAGTA+TGG + Chr5:20321791-20321810 None:intergenic 30.0%
AAATGGAACAAGAGTAGTAT+GGG + Chr5:20321790-20321809 None:intergenic 30.0%
AATAAGGATCAAATCGATTG+AGG + Chr5:20322161-20322180 None:intergenic 30.0%
AATGTATCAACAAGTGAATG+AGG + Chr5:20321556-20321575 None:intergenic 30.0%
ACTTTGAATCAGTTTCATGT+CGG - Chr5:20322392-20322411 MsG0580025548.01.T01:CDS 30.0%
AGGTTCAATTATTGAGAAAG+AGG - Chr5:20321462-20321481 MsG0580025548.01.T01:CDS 30.0%
ATTATATAAGCCTAGACTTG+TGG + Chr5:20322467-20322486 None:intergenic 30.0%
CTCATATCATTGTGAGAATA+TGG - Chr5:20321619-20321638 MsG0580025548.01.T01:intron 30.0%
GCTGTTTATGACTTTAAACA+TGG - Chr5:20321484-20321503 MsG0580025548.01.T01:intron 30.0%
GTAATGCAATAGTATGTGTT+TGG - Chr5:20322919-20322938 MsG0580025548.01.T01:intron 30.0%
GTCCAATTATTCATGATAGA+GGG - Chr5:20321506-20321525 MsG0580025548.01.T01:intron 30.0%
TAGTTGTAATCCGATTGTTA+CGG - Chr5:20322286-20322305 MsG0580025548.01.T01:intron 30.0%
TTCCCTCTATCATGAATAAT+TGG + Chr5:20321511-20321530 None:intergenic 30.0%
! GGCAAAGTTTTTGTTAAATG+CGG - Chr5:20321963-20321982 MsG0580025548.01.T01:intron 30.0%
!! GAAATTTTAAGAGACAACAG+AGG + Chr5:20322971-20322990 None:intergenic 30.0%
!! GAAGCAGTATTTTTCAATCT+AGG - Chr5:20321442-20321461 MsG0580025548.01.T01:CDS 30.0%
AAGACTTCTACCGTAACAAT+CGG + Chr5:20322299-20322318 None:intergenic 35.0%
AGACTTGTGGCAAATTTGAA+TGG + Chr5:20322454-20322473 None:intergenic 35.0%
CACTGCATGCATATAAAAGT+TGG + Chr5:20322548-20322567 None:intergenic 35.0%
CTAGAGAAGTGATTCACTAA+GGG - Chr5:20322758-20322777 MsG0580025548.01.T01:intron 35.0%
CTTAGTGAATCACTTCTCTA+GGG + Chr5:20322759-20322778 None:intergenic 35.0%
GAATATTAGAGTAGTATGGG+TGG + Chr5:20321698-20321717 None:intergenic 35.0%
GGTCCAATTATTCATGATAG+AGG - Chr5:20321505-20321524 MsG0580025548.01.T01:intron 35.0%
GTAGTTCAAGACAAAAACTC+AGG - Chr5:20321861-20321880 MsG0580025548.01.T01:intron 35.0%
GTTGGACATGTGTTATAACT+AGG - Chr5:20321922-20321941 MsG0580025548.01.T01:intron 35.0%
TCATCGGTATTATGTGTGAT+CGG - Chr5:20322835-20322854 MsG0580025548.01.T01:intron 35.0%
TGTGTTATAACTAGGACTAC+TGG - Chr5:20321930-20321949 MsG0580025548.01.T01:intron 35.0%
TTCAAATTTGCCACAAGTCT+AGG - Chr5:20322454-20322473 MsG0580025548.01.T01:intron 35.0%
! AATGAATTGGTTGGTTCAAC+TGG - Chr5:20322114-20322133 MsG0580025548.01.T01:CDS 35.0%
! CTTTTATATGCATGCAGTGA+TGG - Chr5:20322549-20322568 MsG0580025548.01.T01:intron 35.0%
! TAAACCAATAAGCTTTGTGC+AGG - Chr5:20321832-20321851 MsG0580025548.01.T01:intron 35.0%
!! GATGGAGTTGTTCCATTTTT+AGG - Chr5:20322643-20322662 MsG0580025548.01.T01:intron 35.0%
!!! TGTTCCATTTTTAGGACTGA+AGG - Chr5:20322651-20322670 MsG0580025548.01.T01:intron 35.0%
AAATCCACTTAGCCAGTGTT+TGG + Chr5:20321169-20321188 None:intergenic 40.0%
ACAAAAACTTTGCCAGCATC+AGG + Chr5:20321957-20321976 None:intergenic 40.0%
AGGACCTTCAGTCCTAAAAA+TGG + Chr5:20322658-20322677 None:intergenic 40.0%
AGTGAAACATTCTGCAAGTG+TGG - Chr5:20322615-20322634 MsG0580025548.01.T01:intron 40.0%
ATTGAGAGTAGCAGTGACAA+TGG - Chr5:20322591-20322610 MsG0580025548.01.T01:intron 40.0%
CCTAGAGAAGTGATTCACTA+AGG - Chr5:20322757-20322776 MsG0580025548.01.T01:intron 40.0%
CCTTAGTGAATCACTTCTCT+AGG + Chr5:20322760-20322779 None:intergenic 40.0%
CTTTCCTGCACAAAGCTTAT+TGG + Chr5:20321839-20321858 None:intergenic 40.0%
TCACTGCTACTCTCAATTTC+AGG + Chr5:20322588-20322607 None:intergenic 40.0%
TGTGTGATCGGCTATGAATT+TGG - Chr5:20322847-20322866 MsG0580025548.01.T01:intron 40.0%
TTATATGCATGCAGTGATGG+TGG - Chr5:20322552-20322571 MsG0580025548.01.T01:intron 40.0%
! ATAAGCTTTGTGCAGGAAAG+TGG - Chr5:20321839-20321858 MsG0580025548.01.T01:intron 40.0%
! TCACTTCTCTAGGGACAATT+TGG + Chr5:20322750-20322769 None:intergenic 40.0%
AAAAACTCAGGCCTGGGTTT+GGG - Chr5:20321873-20321892 MsG0580025548.01.T01:intron 45.0%
AAAGCTGCACTCAAGAGCAA+GGG - Chr5:20321648-20321667 MsG0580025548.01.T01:intron 45.0%
ACATTCTGCAAGTGTGGTGA+AGG - Chr5:20322621-20322640 MsG0580025548.01.T01:intron 45.0%
CAAGACAAAAACTCAGGCCT+GGG - Chr5:20321867-20321886 MsG0580025548.01.T01:intron 45.0%
GAGGTAGAGCCTTGAAAAGT+CGG + Chr5:20321537-20321556 None:intergenic 45.0%
GTGTTGTGCAAGACTGGTAA+AGG - Chr5:20321900-20321919 MsG0580025548.01.T01:intron 45.0%
TCAAGACAAAAACTCAGGCC+TGG - Chr5:20321866-20321885 MsG0580025548.01.T01:intron 45.0%
TGGAACAAGAGTAGTATGGG+TGG + Chr5:20321787-20321806 None:intergenic 45.0%
TGTGCAAGACTGGTAAAGGT+TGG - Chr5:20321904-20321923 MsG0580025548.01.T01:intron 45.0%
TTGCTCTTGAGTGCAGCTTT+GGG + Chr5:20321648-20321667 None:intergenic 45.0%
! AGGGAAGTTCCGACTTTTCA+AGG - Chr5:20321525-20321544 MsG0580025548.01.T01:intron 45.0%
!!! ATTTTTAATCTTAAATTTAT+CGG + Chr5:20322355-20322374 None:intergenic 5.0%
ATCACTGCTGCACCAAACAC+TGG - Chr5:20321154-20321173 MsG0580025548.01.T01:three_prime_UTR 50.0%
CAAAAACTCAGGCCTGGGTT+TGG - Chr5:20321872-20321891 MsG0580025548.01.T01:intron 50.0%
CAAAGCTGCACTCAAGAGCA+AGG - Chr5:20321647-20321666 MsG0580025548.01.T01:intron 50.0%
CTTGCTCTTGAGTGCAGCTT+TGG + Chr5:20321649-20321668 None:intergenic 50.0%
GTGTGTGACATACAGTGTGC+AGG + Chr5:20322000-20322019 None:intergenic 50.0%
TCTGCAAGTGTGGTGAAGGA+TGG - Chr5:20322625-20322644 MsG0580025548.01.T01:intron 50.0%
! TGCACCAAACACTGGCTAAG+TGG - Chr5:20321162-20321181 MsG0580025548.01.T01:three_prime_UTR 50.0%
ACAACACACTGCCCAAACCC+AGG + Chr5:20321887-20321906 None:intergenic 55.0%
ACGGCATCGTAACGCGAGAT+TGG + Chr5:20321071-20321090 None:intergenic 55.0%
AGGACTACTGGACCTGATGC+TGG - Chr5:20321942-20321961 MsG0580025548.01.T01:intron 55.0%
CCTGATGCAGACTCAGGCAA+GGG - Chr5:20322675-20322694 MsG0580025548.01.T01:intron 55.0%
GAAGGTCCTGATGCAGACTC+AGG - Chr5:20322669-20322688 MsG0580025548.01.T01:intron 55.0%
GGCAGTGTGTTGTGCAAGAC+TGG - Chr5:20321894-20321913 MsG0580025548.01.T01:intron 55.0%
TCCTGATGCAGACTCAGGCA+AGG - Chr5:20322674-20322693 MsG0580025548.01.T01:intron 55.0%
CCCTTGCCTGAGTCTGCATC+AGG + Chr5:20322678-20322697 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr5 gene 20321044 20323010 20321044 ID=MsG0580025548.01;Name=MsG0580025548.01
Chr5 mRNA 20321044 20323010 20321044 ID=MsG0580025548.01.T01;Parent=MsG0580025548.01;Name=MsG0580025548.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0.33|0.25|1|0.33|0.25|4|267|139
Chr5 exon 20321044 20321488 20321044 ID=MsG0580025548.01.T01:exon:8896;Parent=MsG0580025548.01.T01
Chr5 exon 20322048 20322210 20322048 ID=MsG0580025548.01.T01:exon:8895;Parent=MsG0580025548.01.T01
Chr5 exon 20322385 20322456 20322385 ID=MsG0580025548.01.T01:exon:8894;Parent=MsG0580025548.01.T01
Chr5 exon 20323004 20323010 20323004 ID=MsG0580025548.01.T01:exon:8893;Parent=MsG0580025548.01.T01
Chr5 CDS 20323004 20323010 20323004 ID=MsG0580025548.01.T01:cds;Parent=MsG0580025548.01.T01
Chr5 CDS 20322385 20322456 20322385 ID=MsG0580025548.01.T01:cds;Parent=MsG0580025548.01.T01
Chr5 CDS 20322048 20322210 20322048 ID=MsG0580025548.01.T01:cds;Parent=MsG0580025548.01.T01
Chr5 CDS 20321311 20321488 20321311 ID=MsG0580025548.01.T01:cds;Parent=MsG0580025548.01.T01
Chr5 three_prime_UTR 20321044 20321310 20321044 ID=MsG0580025548.01.T01:three_prime_utr;Parent=MsG0580025548.01.T01
Gene Sequence

>MsG0580025548.01.T01

ATGTTTGCAAAAGAAATCAATCATACTACTCATATCATTGTGAGAATATGGAGTCCCAAAGCTGCACTCAAGAGCAAGGCTTTGTGCAGGAAAGTGGTAGTTCAAGACAAAAACTCAGGCCTGGGTTTGGGCAGTGTGTTGTGCAAGACTGGTAAAGGTTGGACATGTGTTATAACTAGGACTACTGGACCTGATGCTGGCAAAGTTTTTGTTAAATGCGGTGAAAATTGCACCTGCACACTTGATGGTGGAGCTGTGACACCTGAAATTGAGAGTAGCAGTGACAATGGCAGTGAAACATTCTGCAAGTGTGGTGAAGGATGGAGTTGTTCCATTTTTAGGACTGAAGGTCCTGATGCAGACTCAGGCAAGGGATTTGCTGATTGCACTCAAGAGTATAACTGTGCTTGTAAATGTTGA

Protein sequence

>MsG0580025548.01.T01

MFAKEINHTTHIIVRIWSPKAALKSKALCRKVVVQDKNSGLGLGSVLCKTGKGWTCVITRTTGPDAGKVFVKCGENCTCTLDGGAVTPEIESSSDNGSETFCKCGEGWSCSIFRTEGPDADSGKGFADCTQEYNCACKC*